Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for enriching and extracting sulforaphane from brassica vegetable seeds

A technology of sulforaphane and extraction method, which is applied in the field of food biology, can solve the problems of low product yield, high toxicity, and high production cost, and achieve the effects of increasing extraction rate, accelerating hydrolysis, and simplifying the production process

Inactive Publication Date: 2012-07-18
NANJING AGRICULTURAL UNIVERSITY
View PDF8 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a technology for the enrichment and extraction of sulforaphane in Brassica vegetable seeds, to solve the technical problems of high production cost, low product yield, and high toxicity in the previous extraction methods of sulforaphane

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] After crushing broccoli seeds with germination ability, add 0.2mol / L Tris-HCl buffer solution according to the ratio of material to liquid (w / v) 1:5, adjust the pH to 6.0, and make an incubation solution; add 0.2mmol / L vitamin C and 0.6mmol / L Zn 2+ Afterwards, incubate at 50°C for 2 hours; add 2.0mmol / L sodium chloride to the incubation solution, extract once with an equal volume of ethyl acetate, and combine the extracts. The extract was filtered and concentrated in vacuo at 45°C to a buttery liquid (or evaporated to dryness). Add ethyl acetate solution to the liquid concentrated in vacuum, and ultrasonically extract at 20°C for 10 minutes (ultrasonic frequency: 90kHz, power: 140W) to obtain a crude extract of sulforaphane, pass through a 0.45μm filter membrane, and vacuum at 30°C Concentrated, obtained.

Embodiment 2

[0028] After crushing the cabbage seeds with germination ability, add 0.2mol / L Tri-HCl buffer solution according to the ratio of material to liquid (w / v) 1:4, adjust the pH to 6.5, and make an incubation solution; add 0.4mmol / L Vitamin C and 1.5mmol / L Zn 2+ Afterwards, incubate at 40°C for 4 hours, add 2mmol / L sodium chloride to the incubation solution, extract twice with equal volume of ethyl acetate, and combine the extracts. The extract was filtered and concentrated in vacuo at 40°C to a buttery liquid (or evaporated to dryness). Add ethyl acetate solution to the liquid concentrated in vacuum, and ultrasonically extract at 40°C for 5 minutes (ultrasonic frequency is 90khz, power is 190W) to obtain a crude extract of sulforaphane, pass through a 0.45μm filter membrane, and vacuum at 40°C Concentrated, obtained.

Embodiment 3

[0030] After crushing cauliflower seeds with germination ability, add 0.2mol / L Tris-HCl buffer solution according to the ratio of material to liquid (w / v) 1:3, adjust the pH to 7.0 to make an incubation solution; add 0.6mmol / L vitamin C and 0.4mmol / L Zn 2+ After incubation at 45°C for 6 h, 1.5 mmol / L sodium chloride was added to the incubation solution, extracted three times with an equal volume of ethyl acetate, and the extracts were combined. The extract was filtered and concentrated in vacuo at 30°C to a buttery liquid (or evaporated to dryness). Add ethyl acetate solution to the liquid concentrated in vacuum, and ultrasonically extract at 30°C for 8 minutes (ultrasonic frequency: 90kHz, power: 170W) to obtain a crude extract of sulforaphane, pass through a 0.45μm filter membrane, and vacuum at 35°C Concentrated, obtained.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for enriching and extracting sulforaphane from brassica vegetable seeds and belongs to the technical field of food biological technology. The method is characterized by comprising the following steps of: taking the sulforaphane brassica vegetable seeds with germinating ability as raw materials; crushing the raw materials and adding a Tris-HCl buffer solution according to the ratio of the materials to liquid being (w / v) 1:(3-5); adjusting the pH value to 6.0-8.0 to form an incubation buffer; adding vitamin C and Zn<2+>, activating endogenous myrosinase in the vegetable seeds and hydrolyzing glucosinolate to form the sulforaphane with an anti-cancer effect; and extracting the sulforaphane from the incubation buffer with ethyl acetate and carrying out filtering, vacuum concentration, ultrasonic extraction and vacuum condensation to obtain a sulforaphane product. The method disclosed by the invention has the characteristics of wide application range of the raw material, low production cost, high enrichment quantity of the sulforaphane and the like. The method is suitable for health-care food sulforaphane products.

Description

1. Technical field [0001] The invention relates to a method for enriching and extracting sulforaphane in Brassica vegetable seeds, in particular to a method for enriching and extracting sulforaphane in vegetable seeds such as broccoli and cabbage, and belongs to the field of food biotechnology. 2. Background technology [0002] Epidemiological studies have shown that eating cruciferous Brassica vegetables, such as broccoli, collard greens, Brussels sprouts, cauliflower, and cabbage, can prevent pancreatic cancer, lung cancer, rectal cancer, breast cancer, and prostate cancer. This is mainly related to sulforaphane, the hydrolysis product of glucosinolates (glucosinolates) in Brassica vegetables. Sulforaphane is an isothiocyanate formed by the hydrolysis of 4-methylsulfinylbutyl glucosinolate (Glucoraphanin, GR, belonging to aliphatic glucosinolates) catalyzed by myrosinase. Plants rich in glucosinolates produce biologically active sulforaphane after digestion in the human b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P13/00C07C331/20
Inventor 顾振新顾颖娟郭强晖尹永褀韩永斌
Owner NANJING AGRICULTURAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com