257 results about "Glucosinolate" patented technology

The invention describes a method of producing extracts from the seeds of meadowfoam, brassicas and crambe plants. A number of subsidiary processes and steps are shown in order to extract differing fractions of oil. Products produced from the above method are also described including uses and methods of these products which include a variety of skin conditions including eczema, facial eczema, dermatitis, external ulcers, welts, rashes, insect bites, allergic reactions and other irritations, burns, wounds, psoriasis, acneiform eruptions, dryness, dry skin, irritation, skin atrophy, secondary infections and the like. The extracts are also described as being a useful compound for treatment of the symptoms of such skin conditions as described above. In particular the use and extraction of glucosinolate (GSL), thiocyanates (TCL) and isothiocyanates (ITCL) is described.

The present invention relates to methods of preventing or inhibiting the growth of Helicobacter through the use of a composition that comprises a glucosinolate, an isothiocyanate or a derivative or metabolite thereof. The present invention also relates to methods of preventing or treating persistent chronic gastritis, ulcers and / or stomach cancer in subjects at risk for, or in need of treatment thereof.

The present invention aims at extracting, separating and purifying the active components of Maka as one natural plant to obtain extractive with several medicinal and health care values. Fresh or dried Maka root material is extracted and separated to obtain active components and extracted residue. The active components includes glucosinolate and its decomposed product isothiocynate in 10-90 wt%,Maka amide and Maka olefin 5-70 wt% and sterol 2-30 wt%. The active components may be further prepared into capsule, tablet, and delayed releasing forms, and the prepared medicine and health food have the functions of treating climacteric syndrome, strengthening male's and female's sexuality, improving sexual function, etc.

Canola plants with high oleic acid and low linolenic acid content are disclosed. In addition, the canola plants of the invention are resistant to imidazolinone herbicides and are resistant to blackleg. The canola plants of the invention also have low total glucosinolate content. There is also provided a method for controlling weeds in a field of canola plants wherein the canola plants are herbicide resistant.

The invention discloses a method for extracting multifunctional sulforaphane from broccoli sprouting vegetable. The method comprises: fresh broccoli sprouting vegetable which grows for 6-10 days is taken as raw material, and is then crushed to be 100 meshes of sieve after being frozen and dried; after that, 100 parts by weight of the processed material is taken to be added with de-ionized water, methylene dichloride, 0.001-0.003 parts by weight of Vc and 0.1-0.3 parts by weight of Na2S, the pH value is adjusted to be 4-6, and the mixed solution is hydrolyzed for 6-10h at 15-35 DEG C; finally, the sulforaphane is obtained after being filtered, washed and purified. As the broccoli sprouting vegetable which grows for 6-10 days is taken as raw material, the method can effectively increase the production rate of the sulforaphane and plays the role of activation for using myrosase to hydrolyze sulpho-glucoside by adding the Vc; meanwhile, sodium sulfide is added for overcoming the interference of Fe in the material, thus improving the production rate of the sulforaphane. The obtained sulforaphane has multiple functions such as cancer resistance, oxidation resistance and the like as well as good application prospect.

The invention provides a preparation method of a maca extractive. The method comprises the steps that maca powder is placed into a microwave dryer, heated to 50-60 DEG C for 15-25min, and cooled to a room temperature; enzyme deactivation treatment is conducted; enzyme activity is destroyed; glucosinolate hydrolysis is prevented; backflow extraction is conducted; water is added to an extractive; the extractive is stirred, stands and is precipitated; supernatant liquid is separated and concentrated; beta-cyclodextrin is added; stirring is conducted for 50-70min; the extractive is embedded; volatilization of glucosinolate is prevented; vacuum drying is conducted; and the maca extractive is obtained. The method can effectively prevent hydrolysis of an available component, namely glucosinolate, in maca, and in particular can effectively prevent volatilization of glucosinolate as the extractive is embedded at normal temperature and pressure; the component is reserved to the greatest extent for improving an effect; a stimulation feel during oral administration is reduced; and the dosage is reduced.

The invention describes a method of producing extracts from the seeds of meadowfoam, brassicas and crambe plants. A number of subsidiary processes and steps are shown in order to extract differing fractions of oil. Products produced from the above method are also described including uses and methods of these products which include a variety of skin conditions including eczema, facial eczema, dermatitis, external ulcers, welts, rashes, insect bites, allergic reactions and other irritations, burns, wounds, psoriasis, acneiform eruptions, dryness, dry skin, irritation, skin atrophy, secondary infections and the like. The extracts are also described as being a useful compound for treatment of the symptoms of such skin conditions as described above. In particular the use and extraction of glucosinolate (GSL), thiocyanates (TCL) and isothiocyanates (ITCL) is described.

The invention discloses a method for removing cotton rapeseed meal toxicant by fermentation method and enhancing nutrient value thereof, a feeding fermented cotton rapeseed meal protein feedstock and applications thereof. The method comprises the steps that: cottonseed meal and rapeseed meal are mixed, the mixture is first added with feedstock water and complex enzyme preparation, and then inoculated with microorganism strain capable of producing protease bacteria or / and lactic acid bacteria as well as decomposing glucosinolate or / and gossypol, then fermentation is performed to obtain treated cotton rapeseed meal so as to obtain nontoxic, highly-nutrient feeding fermented cotton rapeseed meal protein feedstock, and the feeding fermented cotton rapeseed meal protein feedstock is used as protein feedstock component to form cotton rapeseed meal animal feed according to 15% of the addition proportion. Using modern biological technology, the method solves the problems of low utilization rate of cotton rapeseed meal, small amount of addition in the feed, the presence of toxicant and the like, and eases the strain on feed protein in our country.

The invention discloses a preparation method of a maca extract. The method comprises the steps of: (1) maca extract liquid preparation; (2) maca extract liquid pretreatment; (3) ceramic membrane filtration; (4) vacuum concentration; and (5) spray drying. The content of an effective component glucosinolate in the extract provided by the invention can be improved by more than 3 times that of existing methods. Also, with the ceramic membrane filtration step, the physical and chemical characteristics of the maca effective component can be well preserved. With the method, product quality can be improved, production cost can be reduced, and environment can be protected. The method is beneficial for large-scale productions.

The invention discloses a method for preparing aromatic rapeseed oil. The method comprises the following steps: (1) wetting and tempering, namely feeding rapeseeds into a wetting device, spraying water for wetting, and conveying the rapeseeds into a tempering device for tempering; (2) frying and smoking, and removing the glucosinolate decomposition products; (3) pressing to prepare crude rapeseed oil; and (4) crystallizing and filtering the crude rapeseed oil, thereby obtaining the aromatic rapeseed oil. The preparation method disclosed by the invention is simple and easy to operate, any chemical additive does not need to be added in the preparation process, the wastewater and waste gas pollution is avoided, the prepared aromatic rapeseed oil is strong in flavor, the content of total glucosinolate is extremely low, the residual oil content of the rape seed cake is low, and the oil extraction efficiency of the rapeseeds is high.

The invention relates to a deodorized radish red pigment and a deodorization method. The deodorization method comprises the steps as follows: a radish is preheated, crushed and squeezed; pectinase is then respectively added into residues and juices to carry out enzymolysis; filter liquor is combined to obtain radish raw juices; under acid environment, chitosan with certain deacetylation degree isadded into the raw juices, odor of precursor substance glucosinolate is removed, and the purpose of deodorization is finally achieved; red pigment concentrated solution is obtained through separationand concentration, and the radish red pigment powder is obtained through carrying out spray dying for the red pigment concentrated solution. Compared with the traditional deodorization method with ion exchange, macroporous resin adsorption, acid treatment, membrane separation, and the like, the invention has simple technology and high deodorization efficiency, is green and safe, and is suitable for medium and large scale production. The prepared radish red pigment is tasteless, has natural and deep colors as well as favorable water solubility, and can be widely applied to the food and medicine field such as beverages, ciders, candies, and the like.

The present invention discloses a rapeseed meal enzyme-linked micro-ecological preparation and a solid fermentation method of rapeseed meal. The preparation comprises ingredients: xylanase, beta-glucanase, galactosidase, beta-mannanase, cellulase, phytase, acid protease, neutral protease, papain, alkaline protease, amylase, lactic acid bacteria, bacillus, yeasts, starch and polysaccharides. The preparation is prepared by mixing the various enzyme preparations with the fermentation micro-organisms, reduces the use of an acidifier, reduces production costs, and at the same time enhances decomposition and detoxification effects of the preparation. A produced fermented rapeseed meal feed product is rich in 3-15% of lactic acid; glucosinolate is reduced by 80% or more and toxic effects on livestock and poultry are significantly reduced; crude proteins are increased by 1-2% and a consumption of non-protein materials is ensured; a small peptide content is increased to 15-25% and utilization of animals for feed proteins is effectively improved; and bitter substances in the rapeseed meal are effectively removed and a tannin degradation rate is 60% or more.

The invention discloses a breeding method for a new cabbage type rape line, and belongs to the field of a plant new variety breeding method. According to the breeding method disclosed by the invention, a cabbage type rape line is synthesized by interspecific hybridization of cabbages and Chinese cabbages, and the synthesized cabbage type rape line is subjected to quality genetic improvement of economical characters, erucic acid and glucosinolate so as to breed one new ornamental rape line with a bran-new petal color and a bran-new stalk color; the new cabbage type rape line meets both requirements for ornamental value and oil use, and brings greater economic benefits and social benefits for rape production and the tour and sightseeing industry.

The invention is concerned with a kind of rapeseed cakes detoxification reaction mixing filter pot. It relates to electromotor, griddle net and mixing framework inside of the upper pot driven by electromotor. The griddle net set on the inner bottom of upper pot through supporting plate. The upper pot is a column, the down pot is a taper, and the both connect into a whole and each of them has an in-fall, while the down pot has a material exit and a liquid exit. This solves the problem that long filtration time, awfully effect and lots lost of materiel, and so on. Through owning reaction, mixing and filtration, it commendably completes the core technology to prepare concentrated albumen of deep process to rapeseed cakes. Meanwhile, it removes some kinds of poisonous of anti-nutrition matters, such as glucosinolate, polyphenol and phytic acid to get rapeseed concentrated albumen with the nutrition value like fishmeal. It can be added into assorted feed to breed valuable fish.

A manufacturing process of detoxicating concentrating rapeseed protein powder for feed use relates to a detoxication method of rapeseed dregs. The invention takes the defatted rapeseed dregs as raw materials, and then obtains the completed products through middle pressure steam detoxicating and dilute hydrochloric acid detoxicating, separation, ethyl hydrate detoxicating, drying, comminuting and centrifugal separation in sequence. Because the invention has simple process, low water use and alcohol amount and can realize waste water purification and ethanol recovery, so it has low fabricating cost, effective detoxification effect and effective removal of glucosinase, phytic acid and tannin (three toxic harmful substance) in the rapeseed dregs. The products produced by the inventive process has high product quality and comprehensive utilization. So that the invention can be widely used as detoxicating feed raw material and raw materials of phytic acid and tannin.

The invention provides a protein feed prepared by adopting rapeseed dregs, which can effectively reduce the glucosinolate content in the rapeseed dregs by more than 65%. The preparation method for preparing protein feed by the rapeseed dregs comprises the following steps: (1) crushing the rapeseed dregs into rapeseed dreg powder; (2) preparing strains; (3) uniformly mixing and stirring 94-96% of rapeseed dreg powder, 1-3% of modifier, 3-4% of wheat bran, and the strains with clean water, wherein the modifier is formed by mixing 0.1-0.5% of KH2PO4, 0.2-1.5% of urea, 0.05-0.2% of MgSO4, 0.1-0.6% of CuSO4*5H2O, corn flour and / or washing rice bran; (4) filling the uniformly stirred leavening in a fermentation koji tray and arranging the fermentation koji tray on a fermentation koji tray rack; and (5) controlling fermentation temperature to be 28-35 DEG C and fermentation time to be 32-48 hours. The prepared protein feed reduces the glucosinolate content in the original rapeseed dregs by more than 65%, reaches the protein content to 44%, obviously improves the palatability, further rationalizes the amino acid structure, is more beneficial to the digestive absorption of beasts and birds, and has higher feeding value.

The invention discloses a method for detoxicating rapeseed cakes or rapeseed dregs.In the method, the rapeseed cakes or rapeseed dregs containing glucosinolate are ground and detoxicated in a high energy grinder under the action of grinding aid. The method comprises the following steps: 1) weighing rapeseed cakes or rapeseed dregs containing glucosinolate and then weighing grinding aid with a weight being 1-10% of the weight of the rapeseed cakes or rapeseed dregs and then evenly mixing the materials; 2) adding the mixture into the high energy grinder and grinding the mixture for 50-60min to obtain the detoxicated rapeseed cakes or rapeseed dregs.The method of the invention overcomes the weaknesses of the traditional detoxication method such as high cost, complicated processing equipment, long processing cycle, large labor intensity, large energy consumption and low detoxication rate; in addition, the detoxication method features simple process, small equipment investment, convenient operation, high detoxication efficiency, no generation of three wastes in the process of detoxication and adaptability to large-scale industrialized application; moreover the detoxicated rapeseed cakes and rapeseed dregs features good palatability, high digestibility and low content of anti-nutritional factors.

The present invention discloses a breeding method for pod dehiscence-resistant cytoplasmic sterile line and pod dehiscence-resistant cytoplasmic maintainer line of brassica napus, and belongs to a cultivation method for a new crop variety. With the method provided by the present invention, the detection method for pod dehiscence resistance of the rape is improved; the pod dehiscence-resistant germplasm resource is screened; the screened pod dehiscence-resistant line H155 and the cytoplasmic sterile line 077A are subjected to hybridization, and continuous 8 backcrosses to breed the cytoplasmic sterile line H155A, wherein the sterility rate of the H155A is 100%. According to the present invention, the H155A has characteristics of micro pollen at the low temperature, complete sterility at the high temperature, good agronomic characteristics, cold resistance, viral disease resistance and sclerotium disease resistance. The erucic acid content and the glucosinolate content in the H155A is consistent with the erucic acid content and the glucosinolate content in the cytoplasmic maintainer line of the H155A. The pod dehiscence-resistant cytoplasmic sterile line and the pod dehiscence-resistant cytoplasmic maintainer line of the brassica napus establish the foundation for breeding the domestic pod dehiscence-resistant cytoplasmic sterile line hybrids.

The invention discloses a gentle type yellow mustard sauce easy to smear and a preparation method thereof, and belongs to the fruit and vegetable food processing technical field. The product is prepared through activation, preliminary crushing, superfine grinding, heating dissolving, stirring, fermentation, compounding, ultrasonic emulsification and sterilization. Yellow mustard seeds are subjected to low-temperature crushing, so that myrosase activity is protected, enzyme inactivation is avoided from being caused by high temperature, allyl isothiocyanate is fully generated from glucosinolate in the mustard seeds, and thus the production of other bitter substances is reduced. With addition of a right amount of soybean protein, the stability of allyl isothiocyanate hydrolyzed from the mustard powder is improved, and main flavor substance isothiocyanates are not easy to volatilize. With addition of sorbitol, the water activity of the yellow mustard sauce is reduced, hydrolysis of allyl isothiocyanate is inhibited, and the flavor of the yellow mustard sauce is extended. Beta-cyclodextrin is added for embedding allyl isothiocyanate, so that the product is mellower in taste, tastes gentle and does not produce stimulation of nose choking, and the acceptable degree of the product is increased. Intermittent ultrasonic emulsification homogenization makes the yellow mustard sauce be more homogenous and have better smearing property, and flexible sterilization is adopted at a later period so as to better retain the flavor of the product.

The invention provides a new method for detoxifying a rape-seed meal by fermenting saccharomycetes and lactic acid bacteria together. The rape-seed meal has been taken as a feed additive for a long time, but the rape-seed meal cannot be used before being detoxified because the rape-seed meal contains constituents, such as glucosinolate and the like, which are harmful to livestock. Microbial fermentation is a novel detoxification method in conventional rape-seed meal detoxification technology and can overcome the defects of small detoxification range, low detoxification rate, poor palatability of detoxified rape-seed meal and the like in physical and chemical detoxification methods. In the invention, glucosinolate in the rape-seed meal is removed by fermenting saccharomycetes and lactic acid bacteria together; after saccharomycetes and lactic acid bacteria are fermented together in a ratio of 1:1 for 3 to 7 days, the detoxification rate can reach about 60 percent, glucosinolate content can be lowered to about 0.15 percent and protein content of the rape-seed meal is remarkably increased due to microbial fermentation.

The invention discloses a maca extract as well as a preparation method and an application thereof. The maca extract mainly consists of the following materials in percentage by mass: 28-50% of total maca amide, 25-40% of aromatic glucosinolate, 4-12% of total saponins and 1-8% of adenosine, wherein the content of N-benzyl linoleamide in the total maca amide is 35-60% of the content of the total maca amide. The maca extract disclosed by the invention is high in stability, low in content of impurities and rich in various bio-active ingredients; the maca extract has a good effect on improving exercise-induced fatigue resistance and central fatigue resistance; and the maca extract is capable of significantly improving an anti-fatigue effect.

The invention relates to the technical field of microbes, in particular to lactobacillus acidophilus and a screening method and application thereof and a microbial inoculum. The collection number of the lactobacillus acidophilus is CGMCC NO.14437; the lactobacillus acidophilus has a strong glucosinolate degrading function, and can be applied to detoxification of rapeseed meal by independently serving as a fermenting microbial inoculum; when the lactobacillus acidophilus is applied to a rapeseed meal fermenting process, the lactobacillus acidophilus can promote production of polypeptide and lactic acid and has strong abilities of producing acid and inhibiting harmful bacteria; as a broiler feed additive, the lactobacillus acidophilus can promote nutrient digestion and absorption of animals,inhibit proliferation of pathogenic bacteria in gastrointestinal tracts of the animals and promote the balance of intestinal microflora. The lactobacillus acidophilus is a facultative anaerobic microorganism, and is strong in environmental applicability, high in growth speed, tolerable to high-concentration glucosinolate and high in safety.

The present invention relates to a quick determination method of rape-glucosinolate and its test plate, and its technical scheme includes the following steps: using 1-5g or rapeseed sample, pulverizing, using 0.20-0.80g of rapeseed powder and placing it into test tube with plug, adding 1.0-5.0 ml of distilled water, uniformly shaking to produce glucosinolate degradation reaction, after a few minuts filtering, taking 20-80 microlitres of filtrate and placing it on the test plate, adding 60-240 microlitres of potassium dihydrogen phosphate aqueous solution to make reaction, after a few minuts forming colour product, its colour is related to glucosinolate content in sample, adopting spectrophotography to measure glucosinolate content by using measurement instrument according to standard curve. Said invented method is simple and convenient, quick in test speed and low in cost.

The invention provides a rape seed protein feed, which can be solid powder or particles. The feed comprises the following compositions in percentage by weight: 35 to 50 percent of protein (N*6.25, butt), 25 to 33 percent of soluble nitrogen, and 10 to 20 percent of small peptide; and content of glucosinolate is 15 to 25 micromole / gram rape seed protein feed. The invention provides a method for preparing the rape seed protein feed, which comprises the following steps: fermenting crushed rape seed dregs in solid state by a food grade single strain with prolease producing capability; and sterilizing, drying and crushing a fermented culture medium substrate to obtain the rape seed protein feed. The method is friendly to environment in mild reaction condition, and can sufficiently play roles in hydrolyzing the prolease of microorganism and degrading the glucosinolate, so the method is applicable to industrial mass preparation of the rape seed protein feed with low cost.

The invention relates to physiological and biochemical technologies of vegetables, and concretely relates to a method for extracting glucosinolates from wastes comprising stems and leaves of cruciferous vegetables comprising broccoli and the like. The method is characterized in that the wastes of the stems and leaves of the vegetables which are rich in the glucosinolates and comprise broccoli, cauliflower and the like are used as raw materials, and the glucosinolates are extracted through utilizing an anion exchange column. The method has the advantages of changing of wastes into valuables, energy consumption saving, increase of the economic value added of farmlands, and agricultural income increase.

The invention relates to a high-efficiency enrichment technology for isothiocyanate in brassica bud seedlings. The technology is characterized by comprising the following steps: in 5 to 10 d after germination of brassica seeds, subjecting the seeds to heat shock at 40 to 70 DEG C for 10 to 20 min once each day; after harvesting of bud seedlings, grinding and homogenizing the harvested bud seedlings with distilled water according to a material-liquid ratio of 1: 5-10 (w / v), carrying out incubation at 37 DEG C for 3 h and allowing endogenous glucosinolate (thioglycoside) to be thoroughly hydrolyzed so as to produce isothiocyanate with an anticancer effect; and extracting isothiocyanate in a hydrolysate with dichloromethane and carrying out centrifugation so as to prepare an extract rich in isothiocyanate. The technology has the advantages of a wide application scope, simple operation and low production cost. After the brassica bud seedlings have undergone heat shock and homogenization, the content of produced isothiocyanate is 5 to 10 times of the content of isothiocyanate before treatment, and high-efficiency enrichment of isothiocyanate is realized.

A method for preparing GRE by separating from radish seed is carried out by leaching thio-glucoside by boiling methanol, separating and purifying GRE from radish seed coarse extract with countercurrent chromatography as separator. The solvent system consists of n-butyl alcohol, methyl cyanide and 10% ammonia sulfate solution in proportion of 10:3-6:5. It's economical and cheap and has purity above 87%.

Processes for producing a nutritional supplement that contains sulforaphane, and supplements formed thereby. Such a process includes combining a cruciferous vegetable, for example, broccoli sprouts, with at least one strain of Lactobacillus to form a mixture, causing the mixture to undergo lactic fermentation, transform a glucosinolate within the cruciferous vegetable to sulforaphane, and yield a fermented mixture that contains sulforaphane, and then producing from the fermented mixture a supplement that can be ingested by an individual.

Canola plants with high oleic acid and low linolenic acid content are disclosed. In addition, the canola plants of the invention are resistant to imidazolinone herbicides and are resistant to blackleg. The canola plants of the invention also have low total glucosinolate content. There is also provided a method for controlling weeds in a field of canola plants wherein the canola plants are herbicide resistant.

The invention relates to the technical field of food processing and discloses a production process for improving oil yield of healthy pressed rapeseed oil. The production process for improving the oil yield of the healthy pressed rapeseed oil comprises the following steps: (1) selecting rapeseeds, and respectively removing sundries on the rapeseeds by virtue of thick and thin sieves; (2) soaking the screened rapeseeds for 1.5-4 hours with solution; (3) putting the soaked rapeseeds into an ultrasonic instrument to be subjected to ultrasonic treatment for 30-60min for three times; (4) draining the treated rapeseeds, smashing, and carrying out microwave irradiation heating on the crushed rapeseeds, so as to promote oil containing cells to be fully fractured; and (5) after microwave irradiation is finished, steaming and frying for reducing moisture content of the rapeseeds to 5-15%, wherein the rapeseeds are fired with a big fire, after about half hour, the rapeseeds are fired with a small fire and right amount of coarse bran is added and fried together; and when temperature of the rapeseeds reaches 100-150 DEG C, taking out the rapeseeds from a pot, and pressing. The production process provided by the invention can eliminate glucosinolate in the rapeseeds, so that 'green smell' of rapeseed oil is eliminated, and the rapeseed oil is green and healthy; meanwhile, oil yield of the pressed rapeseed oil is improved by 14%, and cost of the rapeseed oil is reduced.