54 results about "Glucosinolate" patented technology

The invention belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a locus qSGC.A8-3 remarkably associated with glucosinolate in brassica napus seeds and application of the molecular marker. A locus qSGC.A8-3 remarkably related to the glucosinolate content of the rape seeds and a peak SNP marker Bn-A08-p12638473 of the locus qSGC.A8-3 are located at 10th, 557th and 348th basic groups of a reference genome A08 chromosome of the rape DarmorV4.1, the average additive effect is 9.6 mu mol / g, and 7.5% of phenotypic variance can be explained. When the PARMS marker designed by the SNP is used for detecting rape germplasm resources, the PARMS marker is simple and convenient to operate and clear in typing, and the average thioglycoside content of AA genotype material seeds is higher than that of GG genotype material seeds, so that the PARMS marker has a good selection effect on the thioglycoside of the rape seeds.

The invention discloses a method for preparing live pig feed through mixed solid-state fermentation of feed rape and rapeseed meal. The method specifically comprises the following steps: harvesting feed rape from a full-bloom stage to a final bloom stage, adding rapeseed dregs, and uniformly mixing to obtain a fermentation substrate with the water content of 55-65%; spraying the compound enzyme bacterium preparation on the fermentation substrate, uniformly stirring, finally compacting by using a packer, sealing, packing and storing, and fermenting for 45 days; the compound enzyme bacterial preparation comprises a bacterial agent and an enzyme agent; the fungicide comprises lactobacillus plantarum, bacillus subtilis, saccharomyces cerevisiae and aspergillus niger; the enzyme agent is prepared from phytase, cellulase, hemicellulase, pectinase, tannase, xylanase and alpha-galactosidase. The feed rape and the rapeseed meal are subjected to mixed solid fermentation, so that the content of phytic acid, glucosinolate and crude fiber is greatly reduced, the content of crude protein is increased, and the nutritional quality of the feed is remarkably improved. The fermented feed is used for replacing part of corn, soybean meal and wheat bran, and the use amount of concentrated feed in live pig breeding is reduced.

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

Pennycress (Thlaspi arvense) seed, seed lots, seed meal, and compositions with reduced glucosinolate content as well as plants that yield such seed, seed lots, seed meal, and compositions are provided. Methods of making and using the pennycress plants and / or seed that provide such seed, seed lots, seed meal, and compositions are also provided.

The invention belongs to the technical field of plant molecular breeding, and discloses an SNP molecular marker related to the glucoraphanin content of leaf mustard, a primer, a kit and application, the SNP molecular marker is located at the B01 chromosome 29599150bp position of the leaf mustard, the basic group of the site is G or A, the flanking sequence of the site is shown as SEQ ID NO: 1, the primer is shown as SEQ ID NO: 2-SEQ ID NO: 4, and the kit comprises the primer; the application comprises the application in identifying leaf mustard germplasm with the glucoraphanin content or the application in molecular assisted breeding of the glucoraphanin content of the leaf mustard. The invention also discloses a method for screening leaf mustard germplasm with high glucoraphanin content. The molecular marker, the primer and the kit developed by the invention can stably and accurately distinguish germplasm with high and low glucoraphanin content.

The invention discloses an imidazole alkynyl sulfur reagent as well as a synthesis method and application thereof in selective construction of thioglycoside, and the method comprises the following steps: taking brominated imidazole sulfosalt shown in a formula (1) as a reaction raw material, and reacting with alkyne shown in a formula (2) in an organic solvent to obtain the imidazole alkynyl sulfur reagent shown in a formula (3); a carbohydrate compound as shown in a formula (4) and an imidazole alkynyl sulfur reagent as shown in a formula (3) are used as reaction raw materials and react in an organic solvent under the action of alkali to obtain the thioglycoside compound as shown in a formula (5). The method has the advantages of mild reaction conditions, cheap and easily available raw materials, simple reaction operation, and high yield and selectivity; reaction substrates are easy to prepare; the reaction disclosed by the invention can be used for constructing a novel imidazole alkynyl sulfur reagent and a thioglycoside compound, and has wide application prospect and practical value.

The invention provides a glycosyl transferase VcUGT1 mutant and application of the glycosyl transferase VcUGT1 mutant in preparation of quercetin-3 '-O-glucoside, and belongs to the technical field of biological enzymes. The invention provides a mutant I132E of glycosyl transferase VcUGT1, and the mutant I132E of the glycosyl transferase VcUGT1 is obtained by screening from blueberries (Vaccinium corybosum). According to the mutant, the catalytic activity and regioselectivity of a glycosyl transfer reaction can be remarkably improved, and a substrate quercetin can be efficiently and specifically catalyzed to generate a corresponding 3 '-O-glucoside compound. According to the enzyme catalytic reaction of the mutant disclosed by the invention, quercetin-3 '-O-glucoside with high medical value can be industrially produced in a large scale, and the catalytic reaction is mild in condition, green and environment-friendly, and has industrial development and application prospects.

Pennycress (Thlaspi arvense) seed, seed lots, seed meal, and compositions with reduced glucosinolate content as well as plants that yield such seed, seed lots, seed meal, and compositions are provided. Methods of making and using the pennycress plants and / or seed that provide such seed, seed lots, seed meal, and compositions are also provided.

The invention belongs to the technical field of molecular breeding, and particularly relates to a rape SNP molecular marker combination, a 4K liquid phase breeding chip and application of the rape SNP molecular marker combination. The rape SNP molecular marker combination comprises 4387 significantly associated SNP loci aiming at seven key agronomic traits, namely the oil content, the oleic acid content, the protein content, the thousand seed weight, the thioglycoside content, the seed coat color and the single plant yield of the cabbage type rape, and specifically comprises 596 effective foreground loci and 3791 background loci. The rape SNP molecular marker combination can realize accurate detection and genotype identification aiming at seven key agronomic traits. A sample test verifies that the 4K liquid phase breeding chip has the advantages of high site pertinence, high detection accuracy and cost adaptation to breeding practice, can be used for molecular marker-assisted selection of rape varieties, remarkably shortens the cultivation period of high-quality and high-yield rape varieties, and is suitable for a large-scale rape breeding scene.

The invention belongs to the technical field of animal feed processing and preparation, and particularly relates to an optimization method of a rapeseed meal treatment process capable of improving nutritional value, the treatment process and a rapeseed meal treatment product. The optimization method comprises the following steps: designing an orthogonal test of the puffed rapeseed meal and carrying out a corresponding orthogonal test according to orthogonal test factors to obtain an optimal parameter combination of the influence factors; processing the rapeseed dregs according to the optimal parameters, and exploring the change of each component of the rapeseed dregs before and after puffing; and processing the rapeseed dregs according to the optimal parameters, and exploring the change of the microstructure and the protein secondary structure of the rapeseed dregs before and after puffing. The glucosinolate, phytic acid, crude fiber content and crude protein in-vitro digestibility are used as evaluation indexes, the treatment effect of the puffing process on the rapeseed dregs is explored, the treatment process of the rapeseed dregs is further optimized through tests, treatment products related to the rapeseed dregs are enriched, and the nutritional value and comprehensive utilization degree of the rapeseed dregs are improved.

The application belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a site significantly related to Brassica napus seed glucosinolate and application qSGC.A8-1 The site significantly related to the content of Brassica napus seed glucosinolate qSGC.A8-1 and a peak SNP marker Bn-A08-p2660414 thereof, which is located at the 2,088,727th base of the A08 chromosome of the Brassica napus DarmorV4.1 reference genome, can explain 8.8% of the average phenotypic variance and has an average additive effect of-12.0 μmol / g. The detection of the PARMS marker designed by using the SNP on Brassica napus germplasm resources shows that the operation is simple and the typing is clear, and the average glucosinolate content of the seed of the A genotype is lower than that of the G genotype, so the Brassica napus seed glucosinolate has a good selection effect.

The invention belongs to the technical field of biology, and particularly relates to recombinant glucoside hydrolase and application thereof in preparation of rare ginsenoside F2. The recombinant glucoside hydrolase provided by the invention is a further modified product on the basis of specific fusion protein. The recombinant glucoside hydrolase provided by the invention can efficiently catalyze a reaction under a relatively mild condition, and has the characteristics of strong substrate specificity and high conversion rate. In the aspect of reaction efficiency, the enzyme shows excellent catalytic performance: a large amount of ginsenoside Rb1 substrate can be converted into the rare ginsenoside F2 in a short time, the performance is excellent, and the efficient and high-purity preparation of the rare ginsenoside F2 is facilitated.

The invention relates to application of a glucoside compound and a pharmaceutical composition, and relates to the technical field of hemorrhoid treatment drugs. The application comprises application of the glucoside compound in preparation of drugs for treating haemorrhoids. The pharmaceutical composition comprises a glucoside compound and pharmaceutically applicable auxiliary materials.

The invention discloses a method for rapidly detecting broccoli glucosinolate content based on a multispectral image, which comprises the following steps: constructing a training set which comprises the multispectral image of broccoli powder and the corresponding total glucosinolate content; constructing a total thioglycoside quantitative prediction model; training the total thioglycoside quantitative prediction model by using the training set; detecting the content of broccoli glucosinolate by using the trained total glucosinolate quantitative prediction model; according to the method, a total thioglycoside quantitative prediction model can be constructed based on the multispectral image, and high-throughput quick detection of the broccoli freeze-dried sample is realized.

The invention discloses a direct synthesis method of unprotected thioglycoside and a medicine for treating diabetes mellitus by using the unprotected thioglycoside. The invention provides a direct synthesis method of unprotected thioglycoside, which comprises the following steps: adding a sugar donor, disulfide and zinc powder into a Schlenk tube, adding a titanium catalyst, a chlorosilane additive and a molecular sieve into a glove box after 20 minutes by using an oil pump, injecting a solvent under N2 atmosphere, stirring at 50-60 DEG C, detecting the reaction process by using TLC (Thin Layer Chromatography), terminating the reaction when the sugar raw material completely disappears, and filtering to obtain the unprotected thioglycoside. Filtering to remove solid impurities, extracting and collecting an organic phase, carrying out reduced pressure distillation to remove a solvent to obtain a crude product, and then carrying out column chromatography by taking a petroleum ether / ethyl acetate / dichloromethane / methanol solution as a mobile phase to obtain the unprotected thioglycoside. The catalyst used in the invention is CpTiCl3, has high reducing ability and selectivity, and can effectively crack C-OH bonds and generate anomeric free radicals to ensure high efficiency and stereoselectivity of the reaction, so that the alpha-configuration unprotected thioglycoside can be obtained.

The application discloses a kind of based on nutrient supply and demand balance winter rape special fertilizer and fertilization method, belong to the technical field of crop planting, including the following steps: (1) obtain the N, P and K nutrient uptake of aboveground part of rape, calculate and determine the initial proportion of N, P and K nutrient;(2) according to the initial proportion of N, P and K nutrient provided by the fertilizer of the obtained rape growth period in step (1), obtain the correction fertilizer N, P and K nutrient proportion;(3) combined with the correction fertilizer N, P and K nutrient proportion obtained in step (2), determine the best proportion of rape N fertilizer base application, seedling stage topdressing and bud stage topdressing, P fertilizer and K fertilizer are all base application, obtain winter rape special fertilizer compound fertilizer.The fertilization method of the application reduces 20%~25% fertilizer, improves the nitrogen utilization efficiency.At the same time, the average yield of rape is increased by 5.5%~12.6%, the oil content and yield of rape are improved, the content of erucic acid, glucosinolate and other substances and soil bulk density are reduced.

The application discloses a nitrilase mutant PgNIT2-A190I, which is obtained by point mutation of alanine at the 190th position of nitrilase PgNIT2 of Paraburkholderia graminis C4D1M into isoleucine. Accordingly, the inventors also design a recombinant plasmid of a nitrilase mutant PgNIT2-A190I coding gene, and construct a corresponding recombinant genetically engineered bacterium. Researches show that, with 3-butenenitrile and 4-pentenenitrile as substrates, the enzyme activity of the nitrilase and the mutant PgNIT2-A190I of the application is 5.94 U / mg and 6.66 U / mg, 9.95 U / mg and 11.90 U / mg at pH 7.0 and 45 DEG C respectively, and the nitrilase mutant of the application is significantly improved. Under various substrate concentration conditions, the hydrolysis capacity of the mutant PgNIT2-A190I of the application to 3-butenenitrile and 4-pentenenitrile is also higher than that of the nitrilase PgNIT2. Moreover, the mutant xylanase has good pH and temperature tolerance, and has production application potential in efficient hydrolysis of rapeseed meal glucosinolate derived nitrile compounds.

Disclosed is a method for preparing a low erucic acid fragrant rapeseed oil. The method includes subjecting a double low rapeseed to aroma-generating treatment, to obtain an aroma-generated rapeseed, the double low rapeseed including lower than 3 wt % of erucic acid, lower than 45 μmol / g of total glucosinolates, 6-15 μmol / g of 3-butenyl glucosinolate, and 0.4-2.0 μmol / g of 4-methylthiobutyl glucosinolate; mechanically squeezing the aroma-generated rapeseed, to obtain a crude oil; and degumming the crude oil, to obtain the low erucic acid fragrant rapeseed oil.

A process of reducing the glucosinolate content in oilseed meal is disclosed. The oilseed meal is derived from Camelina sativa. The oilseeds are conditioned to degrade some of the glucosinolates. The conditioned oilseeds are pressed to expel oil from the seeds. The press cake fraction of the oilseed is extracted with hexane, desolventized and toasted to reduce the glucosinolate content. Defatted meals with low glucosinolate content made by the process are also described.

The invention belongs to the technical field of fermentation engineering, and particularly relates to a method for two-stage enzyme-bacterium synergistic fermentation of rapeseed meal, a protein feed prepared by the method and application of the protein feed. The method comprises the following steps: taking rapeseed meal and soybean meal as fermentation substrates, and performing two-stage fermentation by using a fermentation inoculant and alkaline protease to obtain the protein feed, the fermentation inoculants comprise bacillus subtilis, plant lactobacillus and saccharomyces cerevisiae; the mass ratio of the bacillus subtilis to the plant lactobacillus to the saccharomyces cerevisiae is (1-2): (1-2): (1-2); the two-stage fermentation comprises an aerobic fermentation stage and an anaerobic fermentation stage; the time of the aerobic fermentation stage is 20-28 hours, and the total time of the two-stage fermentation is 32-84 hours. The content of anti-nutritional factor glucosinolate in the protein feed prepared by the method is remarkably reduced, the content of small peptide and acid soluble protein is remarkably increased, and the nutritional value of the rapeseed meal as a protein feed resource is improved.

The application discloses a KASP molecular marker related to quality traits of Brassica napus and application thereof, and belongs to the technical field of molecular genetics.The application discloses the KASP molecular marker related to quality traits of Brassica napus and a primer thereof, and application of the KASP molecular marker and the primer in genotyping or quality trait identification of Brassica napus.The results show that the application can accurately identify genotypes, has high sensitivity and specificity, and effectively reduces the error rate.The application further discloses a Brassica napus genotyping / quality identification method.The results show that a material with a genotyping result of AA has the characteristics of low erucic acid, low glucosinolate, high oleic acid, high linoleic acid, low arachidic acid and low protein content; a material with a GG type shows high erucic acid, high glucosinolate, low oleic acid, low linoleic acid, high arachidic acid and high protein content.The application can quickly screen out individuals with excellent quality traits in early generations, and avoids the long process of identifying traits after plants mature in traditional breeding.

Provided are methods to control growth of weed plants. The methods involve the application of a liquid formulation comprising a herbicidally effective amount of a thiocyanate preparation or isothiocyanate preparation to the foliage of weed plants. The thiocyanate preparation or isothiocyanate preparation can be provided in the form of a glucosinolate hydrolysate.

The invention discloses a purple cabbage-lycium ruthenicum compound fruit and vegetable juice and a preparation method thereof, and is characterized in that the compound fruit and vegetable juice comprises the following components in percentage by mass: 20%-40% of purple cabbage juice, 0.1%-0.5% of lyophilized lycium ruthenicum aqueous extract, 1%-5% of concentrated apple juice, 1%-5% of white granulated sugar, 0.2%-1.0% of D-sodium erythorbate, 0.02%-0.10% of citric acid, 0.02%-0.10% of vitamin C and the balance of water. The purple cabbage juice, lyophilized lycium ruthenicum aqueous extract and apple juice are completely compounded, additives such as D-sodium ascorbate, citric acid and vitamin C are added into the compounded juice in proportion to be dissolved, and water is added for complementation. And homogenizing, degassing, canning, sterilizing and cooling to prepare the purple cabbage-lycium ruthenicum composite fruit and vegetable juice. The compound fruit and vegetable juice is rich in natural active ingredients such as anthocyanin and thioglycoside, and also has a unique purple cabbage flavor, and a proper amount of apple juice is added, so that the compound fruit and vegetable juice has richer and more delicious taste. In addition, the composite fruit and vegetable juice is bright in color, uniform and stable, does not fade after being placed for a long time, and has a good visual effect and taste.

The invention discloses a tetramethyl alkyl thiourea reagent, a synthesis method thereof and application of the tetramethyl alkyl thiourea reagent in selective construction of thioglycoside compounds, the method takes a compound shown in a formula (1) and an alkyl halide shown in a formula (2) as reaction raw materials, and the tetramethyl alkyl thiourea reagent shown in a formula (3) is obtained through reaction in an organic solvent; the thioglycoside compound is obtained by taking sugar as shown in a formula (4) and a tetramethyl alkyl thiourea reagent as shown in a formula (3) as reaction raw materials and reacting in an organic solvent under the action of alkali. The method has the advantages of mild reaction conditions, cheap and easily available raw materials, simple reaction operation, and high yield and selectivity; reaction substrates are easy to prepare; the reaction provided by the invention can be used for constructing a novel tetramethyl alkyl thiourea reagent and a thioglycoside compound, and has wide application prospect and practical value.

This invention discloses a method for cultivating high-lysine, high-quality protein maize. The method involves transferring an RNAi interference expression cassette that inhibits α-glucosinolate expression and a high-lysine fusion gene expression cassette into the maize genome to cultivate high-lysine, high-quality protein maize. The high-lysine fusion gene expression cassette includes a lysine-rich γ-glucosinolate fusion gene, the nucleotide sequence of which is shown in SEQ ID NO. 7. This invention, while inhibiting α-glucosinolate expression in maize, also specifically expresses a lysine-rich γ-glucosinolate fusion gene. The increased expression of γ-glucosinolate promotes the formation of the maize cuticle, resulting in high-quality, flint maize.

The application discloses a kind of low-pressure steam explosion coupling bacterial enzyme synergistic fermentation of rape straw feed and its preparation method, it is related to agricultural waste resource utilization and feed processing technical field, including the following steps: after cutting, natural sun-dried rape straw is loaded into steam explosion equipment, under the condition of setting, steam explosion is carried out, then instantaneously decompression is obtained Steam explosion rape straw, collect steam explosion liquid, and wait for it to restore normal temperature for standby;Compound bacterial enzyme preparation is dissolved in the collected normal temperature steam explosion liquid, is fully stirred, and forms uniform bacterial enzyme-steam explosion liquid mixed preparation;Bacterial enzyme-steam explosion liquid mixed preparation is evenly sprayed to the surface of steam explosion rape straw, and it is ensured that mixed preparation and straw are fully contacted, evenly distributed while spraying while turning over;After mixing, the material is rapidly loaded into fermentation bag, compacts and seals, fermentation, and is obtained immediately.The application can effectively reduce the crude fiber and glucosinolate content of rape straw, reduce the loss of nutritional ingredients, improve the nutritional value, and promote the low-cost feed utilization of rape straw.

The present invention discloses a carbon glycoside compound, a preparation method thereof and an application in anti-tumor drugs, and belongs to the field of carbohydrate drug synthesis. The structure of the carbon glycoside compound is shown in general formula (I) or (II). The present invention uses fully acetylated rhamnose, glucose, mannose and the like as raw materials, and can efficiently obtain unprotected rhamnose, glucose, mannose and other free radical precursors with excellent reactivity and stability by preparing them into benzyl glucosinolates and removing the ester group in two steps. Under the conditions of visible light, photocatalyst, phosphine reagent, alkali and solvent, they undergo Giese reaction with olefin receptors connected to different drugs, and can directly obtain α-carbon glycosides with novel structure and single configuration. The carbon glycoside compound showed good activity in the inhibitory activity assay on human ovarian teratoma cells (PA-1) and digestive tract tumor cells (KYSE-510), and is expected to be further developed into an anti-tumor drug.

The invention relates to the field of analytical chemistry, and discloses a glucosinolate determination method, which comprises: (1) mixing a sample to be determined with an extraction agent, and carrying out first extraction and first solid-liquid separation to obtain a supernatant; (2) mixing the supernate with a first solvent, and carrying out second extraction to obtain a lower-layer extracting solution; (3) measuring the content of glucosinolate in the lower-layer extracting solution; wherein the extracting agent comprises sodium chloride, sodium sulfate, HCl, methanol and water. The method can inhibit degradation of glucosinolate and further increase the content of glucosinolate in the extracting solution, the accuracy of the detection result is high, and the operation is simple, convenient and efficient.

The invention provides an application of a broccoli BoHXK2LF gene in improving the content of indole glucosinolate in broccoli and the resistance of broccoli to fusarium. The invention relates to an application of an overexpression BoHXK2LF gene. The application is any one of the following applications: A1) an application in improving the resistance of broccoli to fusarium; a2) application in cultivation of fusarium-resistant broccoli; a3) application in increasing the content of new glucose brassinolide in broccoli; a4) in increasing the content of total indole glucosinolate in broccoli; a5) application in increasing the content of total glucosinolate in broccoli; wherein the nucleotide sequence of the BoHXK2LF gene is as shown in SEQ ID NO. 1 (sequence identifier number 1). The resistance of broccoli plants to pathogenic fungi (such as fusarium layered) can be improved. The method has important theoretical significance and practical value for cultivating high-disease-resistance broccoli, reducing pesticide application and promoting agricultural sustainable development.

Provided are plants, cells, tissues, and germplasm thereof comprising one or more targeted alterations of the genomic sequences of the MYB28 gene which encodes a transcription factor and regulates the biosynthesis of glucosinolates, as well as meal made from such plant materials. Also provided are methods and compositions to make altered MYB28 gene variants; breeding methods and methods of identifying and selecting plant materials having the disclosed MYB28 variants.