33 results about "Glucosinolate" patented technology

The application discloses a multi-strain combined solid-state fermentation rapeseed meal probiotic agent and a fermentation method thereof, adopts Bacillus amyloliquefaciens, Saccharomyces cerevisiae and Bacillus subtilis to combine solid-state fermentation rapeseed meal to reduce the content of anti-nutritional factors such as glucosinolates, and comprises the following steps: pretreatment and sterilization of rapeseed meal raw materials, preparation of fermentation seed liquid, solid-state fermentation of probiotics, and preparation of fermented rapeseed meal products. The application firstly uses Bacillus amyloliquefaciens to solid-state ferment rapeseed meal, and combines with Saccharomyces cerevisiae and Bacillus subtilis to add into rapeseed meal to carry out combined fermentation; compared with other technical solutions, the application can greatly reduce the content of anti-nutritional factor glucosinolate in rapeseed meal, and can reduce the content of anti-nutritional factors tannin, phytic acid and crude fiber in rapeseed meal and improve the content of peptides in the rapeseed meal product, various bioactive substances can be produced in the probiotic fermentation process, and the method has the advantages of low cost, simple operation, short time consumption and high efficiency.

Pennycress (Thlaspi arvense) seed, seed lots, seed meal, and compositions with reduced glucosinolate content as well as plants that yield such seed, seed lots, seed meal, and compositions are provided. Methods of making and using the pennycress plants and / or seed that provide such seed, seed lots, seed meal, and compositions are also provided.

The invention belongs to the technical field of plant molecular breeding, and discloses an SNP molecular marker related to the glucoraphanin content of leaf mustard, a primer, a kit and application, the SNP molecular marker is located at the B01 chromosome 29599150bp position of the leaf mustard, the basic group of the site is G or A, the flanking sequence of the site is shown as SEQ ID NO: 1, the primer is shown as SEQ ID NO: 2-SEQ ID NO: 4, and the kit comprises the primer; the application comprises the application in identifying leaf mustard germplasm with the glucoraphanin content or the application in molecular assisted breeding of the glucoraphanin content of the leaf mustard. The invention also discloses a method for screening leaf mustard germplasm with high glucoraphanin content. The molecular marker, the primer and the kit developed by the invention can stably and accurately distinguish germplasm with high and low glucoraphanin content.

The invention provides a glycosyl transferase VcUGT1 mutant and application of the glycosyl transferase VcUGT1 mutant in preparation of quercetin-3 '-O-glucoside, and belongs to the technical field of biological enzymes. The invention provides a mutant I132E of glycosyl transferase VcUGT1, and the mutant I132E of the glycosyl transferase VcUGT1 is obtained by screening from blueberries (Vaccinium corybosum). According to the mutant, the catalytic activity and regioselectivity of a glycosyl transfer reaction can be remarkably improved, and a substrate quercetin can be efficiently and specifically catalyzed to generate a corresponding 3 '-O-glucoside compound. According to the enzyme catalytic reaction of the mutant disclosed by the invention, quercetin-3 '-O-glucoside with high medical value can be industrially produced in a large scale, and the catalytic reaction is mild in condition, green and environment-friendly, and has industrial development and application prospects.

The invention belongs to the technical field of molecular breeding, and particularly relates to a rape SNP molecular marker combination, a 4K liquid phase breeding chip and application of the rape SNP molecular marker combination. The rape SNP molecular marker combination comprises 4387 significantly associated SNP loci aiming at seven key agronomic traits, namely the oil content, the oleic acid content, the protein content, the thousand seed weight, the thioglycoside content, the seed coat color and the single plant yield of the cabbage type rape, and specifically comprises 596 effective foreground loci and 3791 background loci. The rape SNP molecular marker combination can realize accurate detection and genotype identification aiming at seven key agronomic traits. A sample test verifies that the 4K liquid phase breeding chip has the advantages of high site pertinence, high detection accuracy and cost adaptation to breeding practice, can be used for molecular marker-assisted selection of rape varieties, remarkably shortens the cultivation period of high-quality and high-yield rape varieties, and is suitable for a large-scale rape breeding scene.

The application belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a site significantly related to Brassica napus seed glucosinolate and application qSGC.A8-1 The site significantly related to the content of Brassica napus seed glucosinolate qSGC.A8-1 and a peak SNP marker Bn-A08-p2660414 thereof, which is located at the 2,088,727th base of the A08 chromosome of the Brassica napus DarmorV4.1 reference genome, can explain 8.8% of the average phenotypic variance and has an average additive effect of-12.0 μmol / g. The detection of the PARMS marker designed by using the SNP on Brassica napus germplasm resources shows that the operation is simple and the typing is clear, and the average glucosinolate content of the seed of the A genotype is lower than that of the G genotype, so the Brassica napus seed glucosinolate has a good selection effect.

The invention belongs to the technical field of biology, and particularly relates to recombinant glucoside hydrolase and application thereof in preparation of rare ginsenoside F2. The recombinant glucoside hydrolase provided by the invention is a further modified product on the basis of specific fusion protein. The recombinant glucoside hydrolase provided by the invention can efficiently catalyze a reaction under a relatively mild condition, and has the characteristics of strong substrate specificity and high conversion rate. In the aspect of reaction efficiency, the enzyme shows excellent catalytic performance: a large amount of ginsenoside Rb1 substrate can be converted into the rare ginsenoside F2 in a short time, the performance is excellent, and the efficient and high-purity preparation of the rare ginsenoside F2 is facilitated.

The invention discloses a method for rapidly detecting broccoli glucosinolate content based on a multispectral image, which comprises the following steps: constructing a training set which comprises the multispectral image of broccoli powder and the corresponding total glucosinolate content; constructing a total thioglycoside quantitative prediction model; training the total thioglycoside quantitative prediction model by using the training set; detecting the content of broccoli glucosinolate by using the trained total glucosinolate quantitative prediction model; according to the method, a total thioglycoside quantitative prediction model can be constructed based on the multispectral image, and high-throughput quick detection of the broccoli freeze-dried sample is realized.

The application discloses a nitrilase mutant PgNIT2-A190I, which is obtained by point mutation of alanine at the 190th position of nitrilase PgNIT2 of Paraburkholderia graminis C4D1M into isoleucine. Accordingly, the inventors also design a recombinant plasmid of a nitrilase mutant PgNIT2-A190I coding gene, and construct a corresponding recombinant genetically engineered bacterium. Researches show that, with 3-butenenitrile and 4-pentenenitrile as substrates, the enzyme activity of the nitrilase and the mutant PgNIT2-A190I of the application is 5.94 U / mg and 6.66 U / mg, 9.95 U / mg and 11.90 U / mg at pH 7.0 and 45 DEG C respectively, and the nitrilase mutant of the application is significantly improved. Under various substrate concentration conditions, the hydrolysis capacity of the mutant PgNIT2-A190I of the application to 3-butenenitrile and 4-pentenenitrile is also higher than that of the nitrilase PgNIT2. Moreover, the mutant xylanase has good pH and temperature tolerance, and has production application potential in efficient hydrolysis of rapeseed meal glucosinolate derived nitrile compounds.

Disclosed is a method for preparing a low erucic acid fragrant rapeseed oil. The method includes subjecting a double low rapeseed to aroma-generating treatment, to obtain an aroma-generated rapeseed, the double low rapeseed including lower than 3 wt % of erucic acid, lower than 45 μmol / g of total glucosinolates, 6-15 μmol / g of 3-butenyl glucosinolate, and 0.4-2.0 μmol / g of 4-methylthiobutyl glucosinolate; mechanically squeezing the aroma-generated rapeseed, to obtain a crude oil; and degumming the crude oil, to obtain the low erucic acid fragrant rapeseed oil.

A process of reducing the glucosinolate content in oilseed meal is disclosed. The oilseed meal is derived from Camelina sativa. The oilseeds are conditioned to degrade some of the glucosinolates. The conditioned oilseeds are pressed to expel oil from the seeds. The press cake fraction of the oilseed is extracted with hexane, desolventized and toasted to reduce the glucosinolate content. Defatted meals with low glucosinolate content made by the process are also described.

The application discloses a KASP molecular marker related to quality traits of Brassica napus and application thereof, and belongs to the technical field of molecular genetics.The application discloses the KASP molecular marker related to quality traits of Brassica napus and a primer thereof, and application of the KASP molecular marker and the primer in genotyping or quality trait identification of Brassica napus.The results show that the application can accurately identify genotypes, has high sensitivity and specificity, and effectively reduces the error rate.The application further discloses a Brassica napus genotyping / quality identification method.The results show that a material with a genotyping result of AA has the characteristics of low erucic acid, low glucosinolate, high oleic acid, high linoleic acid, low arachidic acid and low protein content; a material with a GG type shows high erucic acid, high glucosinolate, low oleic acid, low linoleic acid, high arachidic acid and high protein content.The application can quickly screen out individuals with excellent quality traits in early generations, and avoids the long process of identifying traits after plants mature in traditional breeding.

The invention discloses a purple cabbage-lycium ruthenicum compound fruit and vegetable juice and a preparation method thereof, and is characterized in that the compound fruit and vegetable juice comprises the following components in percentage by mass: 20%-40% of purple cabbage juice, 0.1%-0.5% of lyophilized lycium ruthenicum aqueous extract, 1%-5% of concentrated apple juice, 1%-5% of white granulated sugar, 0.2%-1.0% of D-sodium erythorbate, 0.02%-0.10% of citric acid, 0.02%-0.10% of vitamin C and the balance of water. The purple cabbage juice, lyophilized lycium ruthenicum aqueous extract and apple juice are completely compounded, additives such as D-sodium ascorbate, citric acid and vitamin C are added into the compounded juice in proportion to be dissolved, and water is added for complementation. And homogenizing, degassing, canning, sterilizing and cooling to prepare the purple cabbage-lycium ruthenicum composite fruit and vegetable juice. The compound fruit and vegetable juice is rich in natural active ingredients such as anthocyanin and thioglycoside, and also has a unique purple cabbage flavor, and a proper amount of apple juice is added, so that the compound fruit and vegetable juice has richer and more delicious taste. In addition, the composite fruit and vegetable juice is bright in color, uniform and stable, does not fade after being placed for a long time, and has a good visual effect and taste.

The application discloses a kind of low-pressure steam explosion coupling bacterial enzyme synergistic fermentation of rape straw feed and its preparation method, it is related to agricultural waste resource utilization and feed processing technical field, including the following steps: after cutting, natural sun-dried rape straw is loaded into steam explosion equipment, under the condition of setting, steam explosion is carried out, then instantaneously decompression is obtained Steam explosion rape straw, collect steam explosion liquid, and wait for it to restore normal temperature for standby;Compound bacterial enzyme preparation is dissolved in the collected normal temperature steam explosion liquid, is fully stirred, and forms uniform bacterial enzyme-steam explosion liquid mixed preparation;Bacterial enzyme-steam explosion liquid mixed preparation is evenly sprayed to the surface of steam explosion rape straw, and it is ensured that mixed preparation and straw are fully contacted, evenly distributed while spraying while turning over;After mixing, the material is rapidly loaded into fermentation bag, compacts and seals, fermentation, and is obtained immediately.The application can effectively reduce the crude fiber and glucosinolate content of rape straw, reduce the loss of nutritional ingredients, improve the nutritional value, and promote the low-cost feed utilization of rape straw.

Provided are plants, cells, tissues, and germplasm thereof comprising one or more targeted alterations of the genomic sequences of the MYB28 gene which encodes a transcription factor and regulates the biosynthesis of glucosinolates, as well as meal made from such plant materials. Also provided are methods and compositions to make altered MYB28 gene variants; breeding methods and methods of identifying and selecting plant materials having the disclosed MYB28 variants.

The present invention relates to an organic fertilizer composition for controlling soil pests, containing, as an active ingredient, glucosinolate extracted from rapeseed meal and horseradish, and a preparation method therefor. The present invention comprises: a pulverizing step of pulverizing rapeseed and leaf mustard; an extraction step of extracting an active ingredient from the pulverized material by using a solvent; and an adsorption step of adsorbing the extract onto zeolite.

This invention provides a microbial fertilizer composition for buffering soil acidity and its preparation method, relating to the field of microbial fertilizer technology. The microbial fertilizer composition comprises modified straw biochar, oyster shell powder, fermented straw material, microbial composite microspheres, and organic fertilizer. The modified straw biochar is obtained by treating straw biochar with acid and then mixing it with a carboxymethyl cellulose-glucosinolate solution. The microbial composite microspheres are obtained by mixing pectin-starch microspheres with fermented Lactobacillus mucilage fermentation broth and a fully gelatinized starch solution. This invention overcomes the shortcomings of existing technologies, effectively buffering soil acidity over a long period while further improving soil fertility and promoting high crop yields.

The invention discloses a method for efficiently preparing arachidic acid and behenic acid by adsorbing and decontaminating raw materials through bio-based calcium oxide, and relates to the field of grease chemical engineering, the method comprises the following steps: S1, high-erucic acid rapeseed oil raw materials are subjected to decontaminating treatment through an adsorption column filled with bio-based calcium oxide, and the bio-based calcium oxide is prepared by calcining aquatic organism shells; s2, carrying out acidic hydrolysis on the impurity-removed raw material to obtain mixed fatty acid; according to the method for efficiently preparing the arachidic acid and the behenic acid by adsorbing and removing impurities from the raw materials through the bio-based calcium oxide, the bio-based calcium oxide is prepared from waste shells, high-value utilization of waste is achieved, and the cost is low. The material has a high specific surface area and abundant mesopores, and shows a selective adsorption capacity far higher than that of common calcium oxide on polar impurities such as thioglycoside and colloid, the removal rate can reach 88% or above, and the purity of the raw materials is guaranteed from the source.

The invention provides the effect of riboflavin for delaying the reduction of the content of antioxidant substances in broccoli after harvesting and the application of riboflavin, aiming at the broccoli treated by riboflavin, the content change of ascorbic acid, glutathione, glucosinolate and vitamin B2 in the storage period of the broccoli is measured, and the content of the antioxidant substances in the broccoli is determined. And a real-time fluorescent quantitative PCR method is utilized to research the expression difference of the metabolism genes related to the antioxidant substances between a riboflavin treatment group and a control group, so that a theoretical basis is provided for delaying the reduction of the content of the antioxidant substances after the broccoli is picked by riboflavin treatment.

The present invention relates to a method for producing oil and defatted meal with a glucosinolate content of less than 30 micromoles / g from seeds of the Brassicaceae family, comprising: (a) grinding and hydrating the seeds in a seed:water ratio of 1:2 to 1:7; (b) heating the seed slurry to between 15°C and 50°C; (c) drying the fatty slurry by centrifuging and heating, or only heating, to a fatty slurry temperature of 70°C to 90°C; and (d) subjecting the resulting fatty meal to solvent extraction, producing a meal that is defatted with solvent and a miscella. The miscella is subjected to a separation of the solvent and crude oil, which is the first product of the method, and the solvent meal is subjected to a separation of the solvent and the dry meal, which is the second product of the method.

This invention discloses cyanidin-3- O The use of a complex (C3GVP–MP) consisting of glucoside-4-vinylphenol (C3GVP) and mannose protein (MP) in the preparation of a medicament for treating and / or preventing melanin synthesis-related diseases. This invention provides a medicament for treating and / or preventing melanin synthesis-related diseases, the medicament containing cyanidin-3- O γ-Glucoside-4-vinylphenol-mannose protein complex. Experiments of this invention show that cyanidin-3- O The 4-glucosinolate-4-vinylphenol-mannose protein complex (C3GVP-MP) can provide nutritional intervention for melanin synthesis and is therefore used to prepare drugs for the treatment and / or prevention of melanin synthesis-related diseases.

The invention discloses a feed rape fermentation compound, a preparation method and a fermentation application method. The feed rape fermentation compound comprises the following components: feed rape, modified beta-cyclodextrin, modified starch, a complex microbial inoculant, potassium iodide and selenomethionine, wherein the complex microbial inoculant comprises aspergillus niger, lactobacillus plantarum and bacillus subtilis. When the glucosinolate of the feed rape is converted into sulforaphene by the aspergillus niger, the modified beta-cyclodextrin and the modified starch have a synergistic effect through electrostatic attraction in an acidic fermentation environment, the sulforaphene is embedded in the modified beta-cyclodextrin, and the modified beta-cyclodextrin-sulforaphene inclusion compound is wrapped by a gel network of the modified starch, so that the glucosinolate of the feed rape is converted into the sulforaphene. The sulforaphene is prevented from being exposed to sulfinyl outside the cavity to be attacked, oxygen is isolated, meanwhile, hydroxyl free radicals are blocked from approaching the sulforaphene, oxidation of the sulforaphene is prevented, and the retention rate of the sulforaphene is increased. Potassium iodide and selenomethionine are added, so that the high-retention-rate sulforaphene is prevented from competitively combining with a sodium-iodine co-transporter of a thyroid cell membrane.

Provided are methods and compositions to control growth of weed plants. The compositions include an herbicidally effective amount of an herbicidally active chemical compound and a thiocyanate, together with an herbicidally acceptable diluent, carrier or excipient. The thiocyanate preparation can be provided in the form of a glucosinolate hydrolysate obtained from Sinapis alba plant material. Methods of making and using the formulations are also provided.

The application belongs to the technical field of molecular biology and genetic breeding, and discloses a molecular marker of a site significantly related to Brassica napus seed glucosinolate and application qSGC.A8-3 The site significantly related to the content of Brassica napus seed glucosinolate qSGC.A8-3 , and a peak SNP marker Bn-A08-p12638473 thereof is located at the 10,557,348th base of the A08 chromosome of the Brassica napus DarmorV4.1 reference genome, has an average additive effect of 9.6 μmol / g, and can explain 7.5% of the phenotypic variance. The PARMS marker designed by using the SNP is used for detecting Brassica napus germplasm resources, and it is found that the operation is simple, the typing is clear, and the average glucosinolate content of the AA genotype is higher than that of the GG genotype, so the Brassica napus seed glucosinolate has a good selection effect.

The application relates to the technical field of feed additives, and particularly discloses a feed additive for laying hens, a feed for laying hens and a preparation method thereof. The feed additive for laying hens specifically comprises the following components in parts by weight: 120-140 parts of ligustrum lucidum, 120-140 parts of angelica sinensis, 100-120 parts of chuanxiong rhizome, 100-120 parts of astragalus root, 80-100 parts of curcuma longa, 80-100 parts of tagetes erecta, 60-80 parts of hippophae rhamnoides, 60-80 parts of hippophae rhamnoides leaf, 60-80 parts of cuscuta chinensis, 60-80 parts of rhodiola rosea, 20-40 parts of cordyceps militaris and 30-50 parts of a stabilizer; the stabilizer is composed of zeaxanthin, glucosinolate and flaxseed gum in a weight ratio of 20-40:10-20:5-10. The feed additive for laying hens and the feed for laying hens provided by the technical scheme can obviously improve the egg production rate of the laying hens and obviously improve the quality of the eggs.

The invention relates to a broccoli preservation method, which comprises: selecting fresh broccoli as a raw material, and sequentially carrying out cleaning, pre-cooling, fresh cutting, centrifugal dewatering, 10-20 s intermittent superheated steam treatment, vacuum packaging, intensive pulse light irradiation and refrigeration. Through intermittent superheated steam pretreatment, compact ball-flower structures of broccoli are properly destroyed, the penetrability of intensive pulse light is improved, and surface and deep double sterilization is realized; through cooperative treatment, the hardness, the content of vitamin C and chlorophyll, the content of glucosinolate and anthocyanin, the content of total phenols and the oxidation resistance of the fresh-cut broccoli can be remarkably improved, no chemical reagent is added in the whole process, energy consumption is low, and the treatment time is short. Compared with broccoli subjected to single superheated steam treatment, single intensive pulse light treatment or untreated broccoli, the method has the advantages that enzymatic browning and microbial contamination can be effectively inhibited, yellowing and wilting are delayed, the shelf life of the fresh-cut broccoli is remarkably prolonged, and the problems that an existing fresh-keeping technology is complex in process, high in cost and poor in quality retention are solved.

The present invention relates to a manufacturing process for glucosinolate, or a derivative, analog, stereoisomer or salt thereof, comprising flowing a stream comprising a thiolated compound from a first reactor into a solution comprising a halo oxime in a second reactor in order to form a thiohydroximate compound, and reacting the thiohydroximate compound with pyridine sulfur trioixde in the second reactor in order to form a sulfated thiohydroximate compound.

This invention relates to the field of rapeseed breeding technology, specifically to a method for selecting "double-low" Chinese rapeseed varieties based on open pollination. The method involves: hybridizing high-erucic acid, high-glucosinolate parent lines with low-erucic acid, low-glucosinolate parent lines to create F1 hybrid combinations; isolating the F1 generation for propagation and open pollination to obtain the F2 generation segregating population; isolating the F2 generation for propagation, selecting individual plants, and determining erucic acid and glucosinolate content; retaining "double-low" individual plants for open pollination based on erucic acid and glucosinolate results to obtain F3 generation seeds; subsequent generations are planted under isolated propagation conditions, and selection is performed within each generation using open pollination according to breeding objectives until genetic equilibrium is reached. The advantages of this invention are: (1) improved selection efficiency and accuracy of erucic acid and glucosinolate traits in Chinese rapeseed; (2) the selected low-erucic acid, low-glucosinolate breeding populations are not generated through self-pollination; (3) improved selection efficiency of other important agronomic traits in Chinese rapeseed.

The invention relates to the technical field of crosslinked polyethylene cables, in particular to a smoke-suppression flame-retardant crosslinked polyethylene cable and a preparation method thereof, and the cable sequentially comprises a cable core, an insulating layer, a shielding layer and a sheath layer from inside to outside; the sheath layer is composed of a base layer, a lug boss and a protective layer, and the sheath layer comprises a functional flame-retardant filler, a difunctional repellent, a cross-linking agent and cross-linked polyethylene in a mass ratio of 8: 1.5-2: 0.3: 10; the difunctional repellent comprises honeycomb modified glucosinolate and modified insect repelling microspheres. The preparation method comprises the following steps: S1, preparing the insulating layer and the shielding layer on the outer surface of the cable core; s2, preparing a base material A, a base material B and a base material C; s3, preparing a base layer; s4, preparing a convex part; and S5, preparing a protection layer so as to prepare a sheath layer, and preparing the cable. The difunctional repellent prepared by the invention enhances the insect repelling effect of the cable, improves the moisture resistance, and prolongs the service life of a sheath material.

The invention relates to the technical field of plant extracts, in particular to a glucoraphanin complex weight management composition which comprises the following components in parts by weight: 30-50 parts of a colon synergistic delivery system composed of a glucoraphanin extract and probiotics, 20-60 parts of collagen peptide, 10-30 parts of dietary fibers, 3-5 parts of cabbage powder and 10-40 parts of acceptable auxiliary materials. The glucoraphanin extract is obtained by adopting a low-temperature enzymolysis process. The glucoraphanin extract adopts an integrated process of low-temperature enzyme extraction, vacuum concentration and micro-capsule embedding, so that the activity loss of the glucoraphanin in the production process is reduced to the maximum extent, and the complex weight management composition relying on the glucoraphanin extract can retain the activity of each component to the maximum extent; the targeted release of the active ingredients in the intestinal tract is realized.