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Rapeseed meal enzyme-linked micro-ecological preparation and solid fermentation method of rapeseed meal

A technology of microecological preparations and rapeseed enzymes, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems affecting feed safety, palatability and nutrient utilization, and enzyme-linked microecological enzymatic fermentation Few technical reports, affecting nutritional value and other issues, to achieve the effect of enhancing decomposition and detoxification, reducing toxicity, and eliminating bitter substances

Inactive Publication Date: 2017-08-18
湖北华扬科技发展有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Rapeseed meal, also known as rapeseed meal, is a by-product of oil extraction from rapeseed. The crude protein content of rapeseed meal is about 37%. It is an important protein feed resource, rich in lysine and trace elements, including calcium, The content of selenium, iron, magnesium, manganese and zinc is higher than that of soybean meal, and the content of phosphorus is twice that of soybean meal. At the same time, it is also rich in sulfur-containing amino acids, which are the nutrients that soybean meal lacks, but the crude fiber content of rapeseed meal is higher than that of soybean meal. High, some up to 18% or more; more than 60% of the total phosphorus content is phytate phosphorus, which is not conducive to absorption and utilization
In addition, rapeseed meal also contains toxic substances and anti-nutritional factors such as glucosinolates, tannins, sinapine, saponin, etc., which not only affect its nutritional value, but also endanger the health of animals and cause animal poisoning if they are directly eaten by animals in large quantities Even death, affecting the safety, palatability and nutrient utilization of feed, thus limiting its use in livestock and poultry diets
[0003] Microbial solid-state fermentation technology is widely used in the feed industry. Its main purpose is to improve product quality through solid-state fermentation. The use of microbial fermentation to detoxify rapeseed cakes can not only effectively degrade the sulfur in rapeseed cakes Glycosides can reduce the toxic effect of rapeseed meal on livestock and poultry. At the same time, many enzymes, vitamins, amino acids and some growth-promoting factors can be added to the fermentation substrate, which improves the utilization rate of rapeseed meal in feed. Among them, the microbial detoxification package The technology is very mature, but there are few reports on enzyme-linked microecological enzymatic fermentation technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Preparation of 1kg enzyme-linked microecology: xylanase 40g, β-glucanase 40g, galactosidase 40g, β-mannanase 40g, cellulase 40g, phytase 40g, acid Protease 100g, neutral protease 100g, papain 100g, alkaline protease 100g, amylase 40g, lactic acid bacteria 50g, bacillus 50g, yeast 50g, starch 150g, polysaccharide 20g.

[0027] Among them, the rapeseed meal enzyme-linked microecological preparation, the xylanase, the enzyme activity ≥ 2 million u / g; the β-glucanase, the enzyme activity ≥ 100,000 u / g; the cellulase enzyme activity ≥ 1 10,000 u / g; phytase activity ≥ 400,000 u / g; protease activity ≥ 100,000 u / g; amylase activity ≥ 5000u / g.

[0028] (2) The total weight of rapeseed meal, straw powder, bran, corncob powder, glucose, and rapeseed meal enzyme-linked probiotics is 100%.

[0029] Coarsely crush the rapeseed meal raw material, add 10% straw powder, 10% bran, 10% corncob powder, 5% glucose for use, and then take 5% to 10% (relative to the dry matter weight of t...

Embodiment 2

[0034] (1) Preparation of 1kg rapeseed meal enzyme-linked probiotics: xylanase 60g, β-glucanase 40g, galactosidase 40g, β-mannanase 40g, cellulase 60g, phytase 60g, acid protease 150g, neutral protease 150g, papain 50g, alkaline protease 50g, amylase 30g, lactic acid bacteria 50g, bacillus 50g, yeast 50g, starch 100g, polysaccharide 20g. The activities of various enzymes meet the requirements specified in the present invention.

[0035] Among them, the rapeseed meal enzyme-linked microecological preparation, the xylanase, the enzyme activity ≥ 2 million u / g; the β-glucanase, the enzyme activity ≥ 100,000 u / g; the cellulase enzyme activity ≥ 1 10,000 u / g; phytase activity ≥ 400,000 u / g; protease activity ≥ 100,000 u / g; amylase activity ≥ 5000u / g.

[0036] (2) The total weight of rapeseed meal, straw powder, bran, corncob powder, glucose, and rapeseed meal enzyme-linked probiotics is 100%.

[0037] Coarsely crush the rapeseed meal raw material, add 5% straw powder, 15% bran, 5...

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Abstract

The present invention discloses a rapeseed meal enzyme-linked micro-ecological preparation and a solid fermentation method of rapeseed meal. The preparation comprises ingredients: xylanase, beta-glucanase, galactosidase, beta-mannanase, cellulase, phytase, acid protease, neutral protease, papain, alkaline protease, amylase, lactic acid bacteria, bacillus, yeasts, starch and polysaccharides. The preparation is prepared by mixing the various enzyme preparations with the fermentation micro-organisms, reduces the use of an acidifier, reduces production costs, and at the same time enhances decomposition and detoxification effects of the preparation. A produced fermented rapeseed meal feed product is rich in 3-15% of lactic acid; glucosinolate is reduced by 80% or more and toxic effects on livestock and poultry are significantly reduced; crude proteins are increased by 1-2% and a consumption of non-protein materials is ensured; a small peptide content is increased to 15-25% and utilization of animals for feed proteins is effectively improved; and bitter substances in the rapeseed meal are effectively removed and a tannin degradation rate is 60% or more.

Description

technical field [0001] The invention relates to the technical field of microecological fermentation, in particular to a rapeseed meal enzyme-linked microecological preparation and a method for solid-state fermentation of rapeseed meal. Background technique [0002] Rapeseed meal, also known as rapeseed meal, is a by-product of oil extraction from rapeseed. The crude protein content of rapeseed meal is about 37%. It is an important protein feed resource, rich in lysine and trace elements, including calcium, The content of selenium, iron, magnesium, manganese and zinc is higher than that of soybean meal, and the content of phosphorus is twice that of soybean meal. At the same time, it is also rich in sulfur-containing amino acids, which are the nutrients that soybean meal lacks, but the crude fiber content of rapeseed meal is higher than that of soybean meal. High, some up to 18% or more; about 60% of the total phosphorus content is phytate phosphorus, which is not conducive t...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/18A23K10/14A23K10/30A23K10/37A23K20/163C12N1/20C12N1/16C12N9/16C12N9/24C12N9/26C12N9/38C12N9/40C12N9/42C12N9/50C12R1/125C12R1/10C12R1/225C12R1/01C12R1/72C12R1/86
CPCC12N1/16C12N1/20C12N9/16C12N9/2405C12N9/2411C12N9/2437C12N9/2465C12N9/2468C12N9/248C12N9/2491C12N9/50C12N9/63C12Y302/01025C12Y304/22002Y02P60/87
Inventor 陶敏毛玲周樱詹志春
Owner 湖北华扬科技发展有限公司
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