197results about How to "Good detox effect" patented technology

The invention discloses a special medium for tissue culture of strawberry stem tips, belonging to the technical field of tissue culture seedling, comprising a first generation medium, a successive medium and a rooting medium, wherein the first generation medium is prepared by adding 6-benzyladenine with the concentration of 0.2-0.5mg / L and naphthylacetic acid with the concentration of 0.1-0.2mg / L to an MS medium; the successive medium is prepared by adding 6-benzyladenine with the concentration of 0.2-0.5mg / L to an MS medium; and the rooting medium is a 1 / 2 MS medium. The invention also discloses a method for producing detoxification seedlings by using strawberry stem tip tissues cultured by the mediums, comprising the steps of heat treatment of strawberry seedlings, material drawing and sterilization, first generation culture, successive culture, rooting culture, transplanting and the like. The strawberry detoxification fast propagation system of the invention is convenient and practical, and has good detoxification effect, high propagation factor, good genetic stability and high regeneration rate, thus being massively used on commercial purpose production.

The present invention relates to a culture method for the detoxication and the tissue culture of the bulb of Fritillaria thunbergii, which belongs to the technical field of breeding the plant seedling and is specially used for breeding the detoxic seedling of Fritillaria thunbergii and the detection of the virus of Fritillaria thunbergii. The method comprises the steps such as the formulation of the culture medium, the culture of the detoxication tissue culture bulb, the detection of the virus ELISA, etc. The present invention has good detoxication effect, high sensitivity of the virus detection, and can guarantee that the detocication daughter bulb is free from virus, thereby effectively controlling the occurrence and the broadcasting of the virus disease; the multiplication of the bulb is fast, and the monthly multiplication rate can reach 4.5 times, thereby being applicable to the factory seedling raising, greatly saving the breeding land of the traditional method and obviously reducing the production cost of Fritillaria thunbergii; the present invention can be applied to the purification and rejuvenation and the development of the species of Fritillaria thunbergii.

A detoxicating fast reproduction method of strawberry by anther culture includes such steps as taking the flower bud of strong strawberry, storing at 4 deg.C in refrigerator for 72 hr, disinfecting with the mixture of alcohol, corrosive sublimate and tween, sucking surface water by aseptic suction paper, taking anther, and sequentially culturing in colli inducing culture medium, regrowing culture medium, secondary culture medium, and rooting culture medium.

The invention discloses a method for preparing fermented virus-free cottonseed meal feed protein. The invention relates to a production process which uses cottonseed meal as raw material and produces the virus-free cottonseed meal feed protein by microbial technology in a transformation way, in particular to a microbial fermenting process of using multi-strains mixed solid to ferment and prepare virus-free cottonseed meal. Fermentation cultures of one strain Saccharomyces cerevisiae which is cultured in an amplification way, one strain Candida tropicalis, one strain Bacillus ubtilis and one strain Lactobacillus are evenly mixed in the proportion of 2 to 1 to 1 to 1 and are inoculated in cottonseed meal (through 40 mesh sieve) with 5 percent of the inoculation amount, wherein, the water content of the cottonseed meal is 50 percent; simultaneously, substrate culture media (5 percent of molasses, 5 percent of bran, 0.5 percent of ammonium sulfate, and the initial pH value is 6.0) are added, evenly mixed, fermented for 72hours at 30 DEG C in aerobic way, dried and sampled at 60 DEG C; finally, the nutrient analysis is carried out on the cottonseed meal before and after fermentation; when weighed and packaged, finished products of the fermented cottonseed meal can be stored for 6 months at a cool and dry place. The fermented cottonseed meal can be used as feeds for beef cattle, chicken, tilapia, etc.

The present invention relates to a method for virus-free tissue culture and rapid propagation of sugarcane belonging to the technical field of plant propagation. The method comprises the following steps that the terminal buds or auxiliary buds of the sugar cane is taken as the explants and the virus-free tissue culture seedlings are propagated in mass after the callus tissue inducement and culture, plant differentiation culture, proliferation culture, radication culture and virus test. The present invention is characterized in that the explants adopted are large and the operation is easy; the inoculation survival rate is high; the detoxification is simple, convenient, fast and thorough; the propagation coefficient per month is 5 to 10 times and the speed is fast which is fit for breeding in factory and commercial production.

The invention discloses a flaxseed detoxification method, which comprises the steps of: taking flaxseeds, flatly spreading the flaxseeds into a layer with thickness of 0.1-10cm, continuously feeding steam for 5-180 minutes under normal pressure, then taking the flaxseeds out and drying the flaxseeds to an extent that the water content of the flaxseeds does not exceed 3 percent to obtain the detoxified flaxseeds. By using the flaxseed detoxification method, not only can the flaxseeds be effectively detoxified, but also the loss of nutritional components is very low. Therefore, the flaxseed detoxification method has a great significance to the comprehensive development and utilization of the flaxseeds, the improvement of product added value and the realization of large-scale industrial production.

A method of tissue culture and rapid propagation of healthy sugarcane seedlings comprises: detoxifying a small amount of cleaned sugarcane mature seed rhizomes with warm of 52 DEG C water for 30 minutes, accelerating germination at 38 DEG C, using auxiliary buds as explants for induction, differentiation and breeding to obtain first generation healthy seedlings, detecting ratoon stunting diseasesby means of PCR (polymerase chain reaction) to detect plantlets with negative results, obtaining a great amount of healthy sugarcane seedlings by means of multiplication culture, performing rooting cultivation, seedling adaptation and transplanting to a sand bed for temporary planting, sampling the seedlings for detecting the ratoon stunting diseases by means of PCR, proving qualification of thehealthy sugarcane seedlings, and transplanting the qualified seedlings to a large land or popularizing planting of the seedlings. The method can improve production efficiency of healthy sugarcane seedlings, twice PCR detection guarantees detoxification effect of the healthy sugarcane seedlings, the whole production process is short, propagation by multiple nursery is not needed, seedlings from tissue cultivation are directly used for production or application, the method has the advantages of high efficiency, superior quality, fastness and the like and meets large-scale factory production requirements of the healthy sugarcane seedlings.

The invention relates to a preparation method of a nontoxic cotton dreg animal feed, and aims to provide a preparation method of a nontoxic cotton dreg animal feed, which can be used for solving the problem of detoxication of cotton dregs and increasing nutritional substances in cotton dregs. According to technical key points, the method comprises the following steps of: (1) preparing a primary strain; (2) preparing a secondary strain; (3) preparing a fermentation substrate; and (4) inoculating the fermentation substrate, adjusting the water content to 45-50 percent, mixing uniformly, fermenting for 3-6 days, and drying. The nontoxic cotton dreg animal feed can be taken as a feed for livestock and aquatic livestock, and belongs to the technical field of preparation of feeds.

The invention relates to a chrome slag decontamination process. The process is characterized in that the barium chloride is dissolved and diluted into solution by water, barium carbonate is dissolved and diluted into suspension, which is put into a ball mill with the chrome slag for mixing grinding; when mixing grinding, the chrome slag is crushed; the hexavalent chromium in the chrome slag is continuously dissolved in the crushing process and is reacted with the barium ion to create the barium chromate not dissolved in the water. The invention has the advantages of short disposal process, simple process, good decontamination effect, large industrialization scale, high automation of production pipeline, trivalence chromium in the chrome slag after disposal not easy to be reverted into hexavalent chromium and the chrome slag after disposal mixed with the cement, lime and sand stone used for the stabilization soil of the earthwork.

The invention provides an oriental lily seed ball virus removing method, which specifically comprises the following steps of: A) stripping of explant growth points, B) heat treatment virus removal, C) cleaning and sterilization of explants, D) acquisition, induction and culture of growth points, E) virus-free culture and F) virus detection. The oriental lily seed ball virus removing method has the advantages that the defects existing in the prior art are overcome, the defects caused by a reason that a single method is used are overcome by comprehensively using a heat treatment virus removing method, a shoot tip culture virus removing method and a callus virus removing method, the probability of virus cross infection can be effectively reduced, the virus removal rate is high, the survival rate is high and the culture period of seed balls can be effectively shortened.

A manufacturing process of detoxicating concentrating rapeseed protein powder for feed use relates to a detoxication method of rapeseed dregs. The invention takes the defatted rapeseed dregs as raw materials, and then obtains the completed products through middle pressure steam detoxicating and dilute hydrochloric acid detoxicating, separation, ethyl hydrate detoxicating, drying, comminuting and centrifugal separation in sequence. Because the invention has simple process, low water use and alcohol amount and can realize waste water purification and ethanol recovery, so it has low fabricating cost, effective detoxification effect and effective removal of glucosinase, phytic acid and tannin (three toxic harmful substance) in the rapeseed dregs. The products produced by the inventive process has high product quality and comprehensive utilization. So that the invention can be widely used as detoxicating feed raw material and raw materials of phytic acid and tannin.

The invention relates to a method for rapid removing three sorts of virus in lily, belonging to biologic technical field, which is characterized in that: the meristematic tissue of stem tip is inoculated in the culture medium with ribavirin; wherein, the size of the stem tip is 0.5 to 0.8mm, and the concentration of the ribavirin is 4 mg / L to 6 mg / L. The invention has the advantages of high detoxication rate, high survival rate and rapid regeneration speed.

A detoxicating synergistic agent for the cotton seed dregs to be used for the feed is prepared from amino acid, ferrous sulfate, stone powder, enzyme preparation, potassium chloride and microbes through proportionally mixing. Its application includes breaking said cotton seed dregs, proportionally mixing it with said detoxicating synergistic agent, and laying aside for 48 hr.

The invention discloses a rapeseed dreg chemical detoxifying device. A first feeding hopper is loaded with a rapeseed dreg to be detoxified. A second feeding hopper is loaded with an acid solution. Adriving cylinder drives a lower sieve to move, so that upper sieve pores on an upper sieve are sealed by the lower sieve. A heating sheet can enter the rapeseed dreg in a filling body and is used fordrying, and a display screen is capable of displaying a temperature value in the filling body in real time. After drying, the acid solution is mixed with the rapeseed dreg, and a stirring motor drivesa stirring paddle to rotate, at the same time, the heating sheet is capable of heating a mixture of the acid solution and the rapeseed dreg. After stirring and soaking, the upper sieve pores are staggered with lower sieve pores and coincident through holes are formed. Liquid is filtered by the through holes, and the detoxified rapeseed dreg is heated and dried, finally through adjusting a size ofthe coincident through holes of the upper sieve pores and the lower sieve pores, the different sizes of the rapeseed dregs are screened. A whole technical process of rapeseed dreg chemical detoxification is completed by one device, the detoxifying effect is good, and processing efficiency is high. The device is used for detoxification processing of the rapeseed dreg.

The invention provides a new method for detoxifying a rape-seed meal by fermenting saccharomycetes and lactic acid bacteria together. The rape-seed meal has been taken as a feed additive for a long time, but the rape-seed meal cannot be used before being detoxified because the rape-seed meal contains constituents, such as glucosinolate and the like, which are harmful to livestock. Microbial fermentation is a novel detoxification method in conventional rape-seed meal detoxification technology and can overcome the defects of small detoxification range, low detoxification rate, poor palatability of detoxified rape-seed meal and the like in physical and chemical detoxification methods. In the invention, glucosinolate in the rape-seed meal is removed by fermenting saccharomycetes and lactic acid bacteria together; after saccharomycetes and lactic acid bacteria are fermented together in a ratio of 1:1 for 3 to 7 days, the detoxification rate can reach about 60 percent, glucosinolate content can be lowered to about 0.15 percent and protein content of the rape-seed meal is remarkably increased due to microbial fermentation.

A disclosed method for detoxifying lily based on embryogenic callus specific induction is characterized by comprising the following steps: 1) performing tissue-culture specific induction on lily scale free of insect diseases, so as to generate embryogenic callus; 2) inducing the embryogenic callus to form a seedling and performing heat processing; 3) peeling shoot tip and performing specific induction on the shoot tip to generate embryogenic callus; 4) again inducing the embryogenic callus to form a seedling and performing propagation; and 5) performing virus detection on the tissue culture seedling obtained in the step 4), so as to obtain the lily detoxified seedling when the detection result is qualified. Through two times of embryogenic callus induction, the lily detoxifying method solves the problems that many detoxifying materials are needed, the detoxified seedling is bad in resistance and detoxifiying is not thorough. The method effectively overcomes the genetic depression problem caused by a relatively high lily plantation frequency, has the characteristics of high detoxifying efficiency, simple operation and low production equipment requirements, and is capable of satisfying demands on large-scale production.

The invention discloses a cultivation method for radix ophiopogonis. The cultivation method comprises the following steps: 1) field selecting and preparing, namely, applying a base fertilizer after a field is selected, performing deep ploughing, airing, flattening, raking, ridging, mulching a black film, and opening holes; 2) seedling selecting; 3) field management; 4) pest preventing and controlling. According to the cultivation method, the radix ophiopogonis is planted by adopting the black film; the black film is good in heat insulating and humidity preserving effects, so that normal growth, even quick and exuberant growth, of the radix ophiopogonis when the air temperature is relatively low can be ensured; meanwhile, the growth of field weeds can also be inhibited by the black film; the scramble for water and fertilizer by the weeds is reduced; enough water and fertilizer supply for the radix ophiopogonis is ensured; after seedlings are selected, the seedlings are subjected to detoxification treatment, so that the disease resistance of the seedlings after the seedlings are planted is obviously enhanced; the survival rate is improved.

The invention relates to venin-derived cytotoxin CTXn for drug rehabilitation and a purification method and application thereof, which is characterized in that: 1. the molecular weight of the CTXn is 6697.429Da; 2. the amino acid sequence of the CTXn is LKCNQLIPPF YKTCAAGKNLCYKMFMVAAP KVPVKRGCID VCPKSSLLVK YVCCNTDRCN. A preparation method comprises the following steps of: collecting a Zhoushan cobratoxin specimen outside; carrying out CTXn separation and purification for the Zhoushan cobratoxin specimen, firstly carrying out gel filtration, secondly selecting a component III for the next step of ion exchange through primary screening, and thirdly carrying out ion exchange which adopts a rapid purification process development system, obtaining single toxic polypeptide CTXn, desalting with a G-50 prepacked column, and freezing to dryness for standby; and measuring the molecular weight of the CTXn, the amino acid sequence thereof and the amino acid molecular weight. The venin-derived cytotoxin CTXn can eliminate the drug thirsting behaviors and withdrawal symptoms of addictive rats for morphine, has the characteristics of remarkable drug effect and small toxic or side effect, is favorable for large-scale production, and has great social meaning and economic value for preventing drug harms.

The invention discloses a process for removing toxic material from acid hydrolysis solution of plant fiber raw material through electrodialysis, belonging to biological energy and green chemical industry field. It comprises following steps: cutting and dedusting plant fiber material at direct current field action in electrodialysis set, which comprises a plurality of anion exchange membrane and a plurality of cation exchange membrane; acid hydrolyzing plant fiber according to a certain solid and liquid ratio, the anion in acid hydrolysis solution moving into concentrated compartment passing through anion exchange membrane; while the cation moving into concentrated compartment passing through anion exchange membrane, so the acid in dilute compartments is removed. The invention can eliminate acid and other ionic foreigh matters from acid hydrolysis solution, greatly reduce the product loss rate and recover acid from hydrolysis solution, and it is characterized by simple device and process, good effect of removing toxic substance, easy for control, low cost and good industrial prospect.

The invention provides a processing method of fried dried beef, and belongs to the technical field of processing of meat products. The processing method comprises the following steps of (1) performing pretreatment: performing acid discharge treatment on beef; (2) performing tenderization and pickling: injecting tenderization fluid into raw beef blocks, performing tenderization, and then performing seasoning and pickling, wherein the tenderization fluid contains suberect spatholobus stem protease of which the mass concentration is 0.3-0.4%, rhizopus of which the mass concentration is 0.01-0.02%, and tea polyphenols of which the mass concentration is 0.03-0.05; (3) performing fermentation treatment: fermenting pickled beef and pickled vegetable juice together; (4) performing roasting treatment; and (5) performing air-drying treatment. According to the processing method disclosed by the invention, a technology is improved, so that a new fried dried beef product which is tender in mouth feel, brownish red in color, rich in fragrance and healthy to eat is obtained.

The invention discloses a detoxification treatment method of gibberellin fermentation bacteria residue, and solves the problem that the gibberellin fermentation bacteria residue is difficult to treat.The method includes detoxification treatment on gibberellin fermentation bacteria residue by using a hydrothermal method and comprises the following steps: 1) putting the gibberellin fermentation bacteria residue into a reaction kettle, then adding water, water and H2O2 or water and a surfactant into the reaction kettle, and finally, uniformly mixing the components; 2) sealing the reaction kettlefilled with the materials, and carrying out detoxification treatment on the gibberellin fermentation bacteria residues by utilizing a hydrothermal method under the conditions of heating and stirring;3) after the reaction is finished, cooling the reaction kettle, opening the valve of the reaction kettle to release air pressure after the reaction kettle is cooled, then opening the reaction kettle,carrying out solid-liquid separation on the reacted material, directly discharging one part of the liquid-phase hydrothermal treatment liquid, and recycling the other part of the liquid-phase hydrothermal treatment liquid, and recycling solid-phase filter residues for use. The method has the characteristics of high detoxification efficiency and environmental protection.

The invention discloses a high-efficient and environment-friendly method for quickly removing gossypol from cottonseed cake. The method comprises the steps of selecting cottonseed cake ingredients; adding the cottonseed cake ingredients at the ingredient-liquid ratio of 1: (10-1): 200g / ml into strong acidic ethanol-water solution in the concentration of 0.01-5.0mol / L or weak acidic ethanol-water solution in the mass percentage concentration of 0.01%-80% which serve as detoxifying liquid; extracting for 1min- 7hours at the temperature of 1-100 DEG C and at the stirring speed of 0-1000r / m; taking out for filtering and separating the cottonseed cake; and drying the cottonseed cake to obtain non-toxic cottonseed cake with gossypol removing rate being 98% or above. The method for removing the gossypol has the advantages of simple process, low cost, quickness and high efficiency. Toxin removing agents are easy to get and prepare, non-toxic, pollution-free, and low in consumption. The method for removing the gossypol has high gossypol removing rate so as to meet the feed standard and greatly improve the value added to the processing and utilization of the cottonseed cake, thereby having a good industrial application prospects.

The invention relates to a rapid propagation method of cassava virus-free seedlings. The method comprises the following steps: step one, collecting explants and carrying out a detoxification pretreatment; step two, disinfecting the surfaces of explants and taking off the stem tips; step three, inducing the stem tips to obtain seedlings; step four, subjecting the seedlings to subculture multiplication culture; step five, carrying out virus detection through a semi-quantitative RT-PCR method; step six, domesticating and transplanting the virus-free tube seedlings. The provided method can effectively remove various viruses and bacteria taking cassava stems as the nutrient source, and obtains virus-free cassava seedlings. The healthy planting, purification and rejuvenation of cassava seedling can be achieved. The occupied space for propagation is small, the propagation speed is quick, and the survival rate of transplanting is high. The provided method can be applied to safe species exchange among different countries and areas, can provide healthy cassava seedlings, reduces the happening and spreading of diseases, and provides technical guarantees for sustainable development of cassava.

The invention relates to application of Lactobacillus casei fermentation liquor to feeds, wherein the collection name of the Lactobacillus casei is Lactobacillus casei lyT-7, the collection unit is China Center for Type Culture Collection (CCTCC), and the collection number is CCTCC M2010197. 10 tons of Lactobacillus casei fermentation liquor obtained through fermentation is dried via spray and then added into detoxified rapeseed meal to prepare the feed additive with microbial content of the Lactobacillus casei being 109 living bacteria / g. After candida and the Lactobacillus casei for fermentation are utilized, not only is the role of detoxification played, but also the content of rapeseed meal protein and the nutritional ingredient of the rapeseed meal are improved; and when the Lactobacillus casei is applied to conventional feeds, the piglet feeding experiments prove that the survival rate, the disease resistance and the weight increment effect are effectively improved.

The invention discloses a method for obtaining virus-free sweet potato seedlings through high-temperature-variable temperature processing, and belongs to the technical field of virus-free sweet potatoseedling culturing. According to the method, high-temperature processing of 40-45 DEG C is conducted on rooted sweet potato seedlings for five hours every day, and the processing is conducted continuously for 30-40 days. The method has the advantages that the passivating effect on viruses is high, a stripped stem tip has a long length, the stripping is easy, the efficiency is high, the growth ofan adventitious bud is fast, the rooting quality of regenerating seedlings is good, the seedlings are strong, and the survival rate is high.

The invention discloses application of cytotoxin (CTX1) from snake venom to preparation of a medicament for rehabilitating. Proved by the experiment, the CTX1 can better eliminate the behavioral sensitization of addict rats by morphine, eliminate the medicament thirst behavior and the abstinence syndrome of rats and has favorable rehabilitating effect. In addition, the invention can improve the function state, the diet and the activities of the addict rats and remarkably increase the weight, thereby recovering normal physiological activity.

A chemical method for detoxicating the tissue-cultured seedlings of lily features that its culture medium for tissue culture contains ribavirin (200-505 mu mol / L). Its advantages are high survival rate and high detoxicating effect.

The invention discloses low-yanide semen armeniacae amarum and a preparation method thereof and belongs to the technical field of primary processing of semen armeniacae amarum. The preparation method comprises the following steps: semen armeniacae amarum is adopted as the raw material and subjected to peeling, debittering and detoxification to obtain almond carpel; the almond carpel is subjected to microwave drying to obtain dry almond carpel; semen armeniacae amarum skin is collected, frozen and crushed, and the crushed semen armeniacae amarum skin is uniformly mixed with a debittering liquid; the mixture is subjected to enzymolysis and twice distillation to obtain a semen armeniacae amarum skin extract; the semen armeniacae amarum skin extract is decolored and then mixed with the dry almond carpel at a certain proportion; the obtained mixture is stir-fried to obtain the semen armeniacae amarum which has the natural flavor of semen armeniacae amarum, is smooth and tidy, high in debittering ratio (98.5-99.8 percent), remarkable in detoxification effect (the cyanide residual quantity is 0.04-0.06 mg / kg) and long in guarantee period (24-36 months). The preparation method is simple in process, short in period, high in efficiency and energy-saving, can realize mass production, achieves the purpose of zero emission of toxic substances in the technical field of primary processing of semen armeniacae amarum, is environmental-friendly and explores a novel shortcut for deep processing of semen armeniacae amarum.

The invention discloses an acne-removing gel capable of inhibiting bacteria, diminishing inflammation, refreshing and benefiting skin, and a preparation method thereof. The acne-removing gel comprises the following components: pine needle oil, grape seed oil, linseed oil, rosemary tea seed oil, rose wheat germ oil, borage oil, corn oil, a cucumber extract, a cortex phellodendri extract, a radix paeoniae alba extract, a bletilla striata extract, a folium artemisiae argyi extract, a kiwi fruit extract, a folium cortex eucommiae extract, a lavender extract, a herba violae extract, aloe vera gel, an ice crystal forming agent AVC, carbomer, sodium alginate, ethylparaben, a 30% triethanolamine solution, distilled water, essence and 1, 2-propylene glycol. Compared with the prior art, the product is fragrant in smell and rapid in absorption, deeply nourishes the skin, regulates skin grease secretion, supplements skin moisture, tightens the skin, improves acne pores, and has various skin care effects of inhibiting bacteria and diminishing inflammation, cleaning acnes and relieving, promoting wound healing, smoothing acne pits, fading acne marks and the like. After long-term use, the skin is smoother, finer, moisturized, tender and glossy.

A heat treating method for detoxicating the tissue-cultured seedlings of lily features that the lily bulb in germination state is treated at 35-50 deg.C and 100% air humidity for 20-40 days. Its advantages are high survival rate and high detoxicating effect.