The invention discloses a fingered citron yam
tissue culture detoxification method, and relates to the technical field of yam detoxification. The method comprises the following specific steps: step 1, taking fingered citron yam vines as experimental materials, taking young and tender stem segments, and cultivating aseptic
seedling materials; step 2, performing
alcohol disinfection on the stem sections with the nodes; step 3, disinfecting the stem segments with the nodes by using a
disinfectant; 4, the stem segments with the nodes are put into a culture medium to be subjected to dark culture and light culture; 5, performing high-
temperature treatment on the rooted aseptic seedlings; and step 6,
cutting the stem tips of the aseptic seedlings after heat treatment, and transferring the stem tips into a culture medium to obtain the
tissue culture virus-free seedlings of the bergamot yams. According to the method,
tissue culture detoxification and cultivation condition optimization are carried out on the bergamot yam
plant tissue, the problem that the bergamot yam tissue culture is prone to
browning is solved, a sterile tissue culture
system of the bergamot yam is perfected, and toxins inside and outside the bergamot yam are effectively reduced. Meanwhile, the aseptic seedlings are subjected to heat treatment, so that stable
virus-free seedlings of the bergamot yams are obtained.