54 results about "Bacillus mojavensis" patented technology

This invention provides compositions of Bacillus strains and methods for using such compositions to inhibit the activity and / or growth of fungal pathogens of plants. In one embodiment, this invention provides a composition comprising Bacillus bacteria selected from the group consisting of Brevibacillus laterosporus strain CM-3, Brevibacillus laterosporus strain CM-33, Bacillus amyloliquefaciens BCM-CM5, Bacillus licheniformis ATCC-11946, Bacillus mojavensis BCM-01, Bacillus pumilus NRRL-1875, Bacillus subtilis 10 DSM-10, Bacillus subtilis NRRL-1650, Bacillus megaterium BCM-07, Paenibacillus polymyxa DSM-36, Paenibacillus chitinolyticus DSM-11030, and combinations thereof. In another embodiment, this invention provides a method for preparing a bacterial composition comprising one or more Bacillus strains by growing Bacillus strain bacteria until the bacteria form spores, collecting said spores, and formulating said composition.

The invention provides a microbial agent and a preparation method thereof as well as application of microbial agent to a wastewater treatment, relating to the technical field of environmental engineering. The microbial agent provided by the invention is prepared from eubacterium dolichum, bacillus coagulans, lactobacillus brevis, bacillus mojavensis, mucor, lactobacillus plantarum and cellvibrio. By means of the intercoordination and interaction of the eubacterium dolichum, the bacillus coagulans, the lactobacillus brevis, the bacillus mojavensis, the mucor, the lactobacillus plantarum and the cellvibrio, a microbe system that can treat domestic wastewater in high efficiency is formed, the microbial agent prepared from the method provided by the invention has properties of degrading grease, starch and cellulose, can effectively reduce the releasing of H2S in excrement, and has higher removal rate on removing COD, BOD5, SS, TP, NH3-N, TN from wastewater.

The invention discloses a method for preparing a biological compound demulsifying agent, which relates to a method for preparing a demulsifying agent. The method solves the problems that the harms to the environment caused by a chemical demulsifying agent still exist although the conventional biological demulsifying agent is compounded with the chemical demulsifying agent or auxiliary agents with other functions, and the biological demulsifying agent has low demulsifying efficiency, poor stability and high cost. The method comprises the following steps: firstly, preparing a bacillus mojavensis seed solution; secondly, preparing a bacillus subtillis seed solution; and finally, mixing the bacillus mojavensis seed solution and the bacillus subtillis seed solution, and inoculating the mixture into a culture medium for culture to obtain the biological compound demulsifying agent. The biological compound demulsifying agent has an oil expulsion rate up to 100 percent, reduces the addition and the cost, has high stability which is shown by good demulsifying effect on emulsions under the condition of the pH value of 3 to 9, and the change of the oil expulsion rate of not more than 10 percent when the temperature of the emulsions changes between 20 and 120 DEG C.

The invention belongs to the technical field of microorganisms, and provides a development and multi-stage strengthening method for a Shanxi mature vinegar composite bacterial agent based on bacteriastrain interaction. Shanxi mature vinegar native high-yield alcohol saccharomyces cerevisiae, high-yield ester wormwood candida, high-yield acetoin, bacillus mojavensis of high ester production capacity, high-yield non-volatile acid lactobacillus plantarum, high-yield acid, acetoin and acetobacter pasteurianus of high tolerance are subjected to an interaction experiment. The saccharomyces cerevisiae, the wormwood candida, the bacillus mojavensis and the lactobacillus plantarum are prepared into a compound direct vat set in the alcoholic fermentation stage, the bacillus mojavensis, the lactobacillus plantarum and the acetobacter pasteurianus are prepared into a compound direct vat set in the fermentation stage for strengthening production of Shanxi mature vinegar, according to new pour vinegar, the content of total acid is 6.23 g / 100mL, the content of involatile acid is 2.68 g / 100 mL, the content of total ester is 5.88 g / 100 mL, the content of ligustrazine is 335.6-5 micrograms / mL, andthe spectrogram and content of organic acid and volatile aroma are enriched.

The invention provides bacillus mojavensis with the preservation number of CCTCC NO. M2013499. The invention also provides a microbial inoculant. The microbial inoculants comprises a medium and bacteria, wherein the bacteria comprise bacillus mojavensis with the preservation number of CCTCC NO. M2013499, and also comprises bacillus subtilis, bacillus lincheniformis and bacillus amyloliquefaciens. The invention also provides application of bacillus mojavensis as described above and the microbial inoculants as described above to saline land improvement. The invention also provides application of bacillus mojavensis as described above and the microbial inoculants as described above to increase plant drought tolerance. By employing the above technical scheme, salts and alkalis can be relatively effectively removed, for example, the soil desalting rate is improved by 55% or more.

The invention discloses an organic garbage deodorant containing microbial strains and a preparation method thereof. The organic garbage deodorant comprises the following components in percentage by weight: 0.01 to 0.05% of ethylene diamine tetraacetic acid, 0.30 to 0.75% of diammonium hydrogen phosphate, 0.05 to 0.20% of biological surfactant, and 99 to 99.5% of fermentation broth, wherein in the active components of fermentation broth, active bacillus mojavensis accounts for 70 to 80% of total active bacteria, and bacillus pumilus accounts for 20 to 30% of total active bacteria. The preparation method comprises the following steps: activating bacterial strains, preparing seed liquid, fermenting the bacterial strains, preserving bacterial strains, and producing finished product of deodorant in sequence. The provided organic garbage deodorant comprises natural active components, can remove the source that gives off odors, and plays a long-lasting and effect deodorant effect.

This invention relates to a new nematicidal product for the biological control of plant pathogenic nematodes containing three new isolates of Bacillus thuringiensis strain N11, Bacillus mojavensis strain SR11 and Azospirillum brasilense strain ALo1, deposited in the Spanish Type Culture Collection (CECT) with the names CECT-7665, CECT-7666 and CECT-5856 respectively, which have a high antagonistic capacity against pathogens of this type, as well as the property of stimulating plant growth through different mechanisms. The invention also relates to liquid and solid formulations of the product which have good stability at room temperature and which are effective in the biological control of plant pathogenic nematodes and for stimulating the growth of plants.

The invention discloses a Bacillus mojavensis strain HMB20199 for controlling cucumber downy mildew, wherein the Bacillus mojavensis strain HMB20199 is preserved in China Microbial Culture Collection Management Center on December 20th, 2011, with the preservation number of CGMCC No.5617. The invention also discloses a microorganism bacterium agent produced by using the HMB20199 and a preparation method of the microorganism bacterium agent. Active components of the microorganism bacterium agent are the Bacillus mojavensis HMB20199 thallus and extracellular metabolites thereof. The HMB20199 strain disclosed by the invention is strong in specificity in controlling of the cucumber downy mildew and high in control efficiency, wherein the average control efficiency is over 85 percent; and moreover, the drug resistance is not easily generated by controlling the cucumber downy mildew through the Bacillus mojavensis strain HMB20199. In addition, the microorganism bacterium agent has no harm topeople or livestock and does not pollute the environment. The preparation method of the microorganism bacterium agent is simple, low in cost and convenient in use.

The invention belongs to the technical field of environment-friendly waste water treatment, and particularly relates to a microbial water treatment agent for heavy metal containing organic waste water. The microbial water treatment agent for the heavy metal containing organic waste water is prepared from dolomite powder, medical stone powder, anabaena azollae powder, water hyacinth powder, cladosporium sphaerospermum, Indian mustard powder, citrobacter amalonaticus, penicillium digitatum, hydroxyapatite powder, zeolite powder, citric acid, succinic acid, dicranopteris linearis powder, rhus lacquer powder, horse radish peroxidase, suillus bovines, laccaria amethystea, phanerochaete chrysosporium, bacillus subtilis, trichoderma koningii, trichoderma viride, bacillus mojavensis and klebsiella variicola. The microbial water treatment agent is high in treatment efficiency, low in running cost, wide in pH and temperature condition range, high in adsorbent reproducibility and free of secondary pollution.

The invention relates to a resourceful treatment method for waste bodies of dead livestock and poultry animals. The resourceful treatment method comprises the following steps: refrigerating, storing and cutting dead animals, and crushing the refrigerated dead animals to obtain animal fragments; cooking the animal fragments; mixing the oil-removed animal fragments with a compound microbial agent to carry out micro-biological degradation; and carrying out medium-temperature aerobic fermentation on a degraded product to obtain biogas, biogas slurry and biogas residues, wherein the compound microbial agent consists of anabaena, bacillus subtilis, lactobacillus acidophilus, bacillus megatherium, bacillus cereus, bacillus pumilus, slender rhodospirillum, stenotrophomonas maltophilia and bacillus mojavensis. The resourceful treatment method solves the problem of treating dead bodies of the livestock and poultry animals of a large-scale breeding plant, so that resources are reused.

The invention provides a Bacillus mojavensis QLY002 strain. The Bacillus mojavensis QLY002 strain has a preservation number of CGMCC No. 8905. The invention also provides a microbial inoculum of the Bacillus mojavensis QLY002 strain and its preparation method and use in potato pathogen inhibition and potato growth promotion. The Bacillus mojavensis QLY002 strain and the microbial inoculum can be used as microbe pesticides and fertilizer, can inhibit Phoma foveata, Alternaria solani, Fusarium oxysporum and Colletotrichum coccodes, have an antibacterial rate more than 61%, have potato growth promotion effects, can be used as potato special-purpose microbial pesticide and microbial fertilizer, solve the problem that the existing chemical pesticide has poor environmental friendliness, easily causes pesticide resistance and has poor safety, and have the advantages of good environmental friendliness, no pesticide resistance and good safety.

Relating to the technical field of surface degreasing of metallic materials, the invention provides a preparation method for a biological component of a biological compound degreaser. The method comprises the steps of: conducting screening, enriching, domestication and culturing respectively to zoogloea, Bacillus mojavensis, bacillus subtilis, Serratia marcescens, then taking each cultured bacterium in proportion so as to prepare the biological component of a biological compound degreaser. The biological component prepared by the selected 4 bacteria in the invention can degrade mixed oil stains of punching oil, cutting oil, lubricating oil, animal oil and a plurality of oil, and can realize rapid and comprehensive degradation as well as excellent degrading effects.

A nematicidal product for the biological control of plant pathogenic nematodes containing three new isolates of Bacillus thuringiensis strain N11, Bacillus mojavensis strain SR11 and Azospirillum brasilense strain ALo1, deposited in the Spanish Type Culture Collection (CECT) with the names CECT-7665, CECT-7666 and CECT-5856 respectively, which have a high antagonistic capacity against pathogens of this type, as well as the property of stimulating plant growth through different mechanisms. Liquid and solid formulations of the product which have good stability at room temperature and which are effective in the biological control of plant pathogenic nematodes and for stimulating the growth of plants.

The present invention relates to the field of bioengineering, in particular to alkaline protease BmP mutants with improved thermal stability and genes and application thereof. The mutants are obtainedfrom a mutant of an alkaline protease BmP by mutating amino acid S at the 193 site into L or K or mutating amino acid S at the 288 site into K or M, wherein the amino acid sequence of the alkaline protease BmP is shown as SEQ ID NO.6. The thermal stability of the alkaline protease BmP of bacillus mojavensis is improved. Under the premise of not destroying other excellent enzymatic properties of the alkaline protease, the thermal stability of the alkaline protease is significantly improved, and a foundation is laid for industrialized application.

The invention relates to bacillus mojavensis and application thereof and belongs to the field of environmental microorganism application. The bacillus mojavensis B1811 is preserved at the general microbiology center of China Committee for Culture Collection of Microorganisms(CGMCC), the preservation number is CGMCCNo.12805, the preservation date is on July 21, 2016. The bacillus mojavensis can be used for degrading dibutyl phthalate. The degradation rate of the dibutyl phthalate can be as high as 99.7%. A method for degrading the dibutyl phthalate by using the bacillus mojavensis B1811 is adopted. In the presence of a yeast extract, the bacillus mojavensis B1811 is in contact with the dibutyl phthalate. Compared with methods for degrading the dibutyl phthalate by using bacteria and fungi, the method for degrading the dibutyl phthalate has a new conception in strain source, has high dibutyl phthalate degradation rate and is short in degradation period and simple to operate.

A process for obtaining gold magnetic nanoparticles or of other metallic elements by fermenting a culture medium in the presence of producer microorganisms such as Brevibacterium halotolerans, Bacillus mojavensis, Bacillus subtilis subsp. Inaquosorum, Bacillus subtilis subsp. Spizizenii, Bacillus tequilensis, Bacillus amyloliquefaciens subsp. Amyloliquefaciens, Bacillus siamenensis, Bacillus amyloliquefaciens subsp. Plantarum, Bacillus subtilis, Bacillus subtilis subsp. Inaquosorum, Bacillus atrophaeus, or Bacillus vallismortis, inter alia. The process of the invention can be used to effectively control the size and shape of the nanostructure to be obtained, with production levels above those found at laboratory level.

The invention discloses a strain of Bacillus mohaevei for promoting the growth of Thoreau grass and the application thereof. The strain KKDD belongs to Bacillus mojavensis (Bacillus mojavensis), is deposited at the China General Microbiological Culture Collection Center on 11 April 2018, strain collection No. CGMCC NO. 15579(address: No. 3 Yuan, No. 1 Beichen West Road, Chaoyang District, Beijing)Bacillus rhizospheres KKDD isolated from saline-alkali vegetation in Hoh Xil, Qinghai province, has that characteristics of nitrogen fixation, antagonism to pathogen, IAA production, germination promotion and saline-alkali tolerance, and has certain research and application potential in grassland vegetation restoration and ecological agriculture and animal husbandry in plateau. The KKDD strain can not only obviously improve the growth of Thoreau grass, but also degrade straw lignocellulose and antagonize the fungal diseases of Thoreau grass.

A biological material is disclosed for exerting antagonism against vegetable pathogens, which contains Bacillus mojavensis R3B mutant strain deposited under # NCAIM (P) B 001389 according to the Budapest Treaty. The biological material according to the invention is an effective antagonist against the pathogens of vegetables, preferably tomato, pepper, lettuce and / or cabbage, in particular against the pathogens selected from the group of Xanthomonas vesicatoria, Pseudomonas syringae and Clavibacter michiganensis vegetable pathogen bacteria and Pythium debaryanum, Phytophthora infestans, Alternaria alternata and Fusarium oxysporum vegetable pathogen fungi. Furthermore, the invention relates to a composition comprising the biological material according to the invention and optionally a copper-containing pesticide and a process for controlling vegetable pathogens, furthermore use of the biological material or composition according to the invention for the protection of vegetables, preferably tomato, pepper, lettuce and / or cabbage.

The invention relates a method for controlling ammonia gas volatilization of sludge composting. The method comprises the following steps: mixing sludge with the humidity of 75 percent to 85 percent, fungus bran and molasses; then composting and fermenting, and forcedly ventilating; fermenting for 48+ / -2h and adding bacillus mojavensis P2 according to the bacterium inoculation amount of 0.8 percentto 1.2 percent; continually fermenting for 18 to 20 days to finish composting, maturation and fermentation. According to the method provided by the invention, the bacillus mojavensis P2 has an assimilation effect with ammonia and the volatilization amount of ammonia gas is reduced; the activity of glutamate dehydrogenase in compost is increased and the loss of nitrogen element in a sludge composting process can be effectively controlled; the effective utilization rate of the nitrogen element is increased. By adopting the method provided by the invention, the volatilization amount of the ammonia gas can be reduced, the problem of sludge composting odor is greatly alleviated and the quality of a composting product is remarkably improved.

The invention discloses a production method of an endophytic bacillus mojavensis solid inoculant and the application of the production method, belongs to the field of microorganism, and aims to solve the problems of difficulty in storage, uneasiness in transportation and secondary fermentation of the conventional liquid fermented bacteria solution. The method comprises the following steps: implanting bacillus mojavensis strain into a solid substrate, so as to be fermented, with the implanting amount of 4 to 12 percent, pH value of 7.0 to 8.0, the fermentation time of 30 to 42h, and the fermentation temperature of 34 to 42 DEG C. The microorganism inoculant fermented through agricultural waste solid is easy to store and transport and convenient to use, and has obvious advantages. The control effect of endophytic bacillus mojavensis to cucumber fusarium wilt and capsicum fusarium wilt is greater than 61 percent, so that the defects of poor environmental protection property, easiness in insecticide resistance production and poor safety, and the endophytic bacillus mojavensis has the advantages of good environmental protection property, uneasiness in insecticide resistance production, good safety and growth promotion (fertilizer) functions, meets the national requirements of pesticide reducing and chemical fertilizer reducing, and is also beneficial to environmental protection.

The invention discloses Bacillus mojavensis for preventing and treating root rot of cotton in a seedling stage and application of the Bacillus mojavensis, belonging to the technical field of agricultural biological prevention and treatment. The Bacillus mojavensis HWt34 disclosed by the invention provides a novel and efficient microorganism for preventing and treating root rot of cotton in the seedling stage; a Bacillus mojavensis wettable powder and a biological fertilizer disclosed by the invention are high in pesticide effect on cotton root rot, have an average control effect of 78.43% or above, and thus have broad-spectrum bactericidal effect; and the Bacillus mojavensis wettable powder and the biological fertilizer are long in lasting period during preventing and treating of the rootrot of the cotton in the seedling stage, do not easily generate drug resistance, and have an obvious promoting effect on cotton growth.

The invention relates to a method for preparing active ginsenoside. The method comprises the steps of treating ginseng with hot steam, and then conducting pressing and ginsenoside extraction with ethyl alcohol, so as to prepare active ginsenoside constituents. During fermentation, two strains (bacillus mojavensis KJS-3 and bacillus polyzyme KJS-2) are treated by means of two temperature steps (45 DEG C and 55 DEG C) in sequence. Saponin solution obtained through centrifugation is separated, and supernate is reserved. Sediment standing is conducted with ethyl alcohol again, and then filtration is conducted. Filtrate is concentrated, frozen and dried to obtain active ginsenoside. By the adoption of the method, high-yield active ginsenoside containing the compound CK can be obtained. Saponin prepared through fermentation can be used in food.

The invention discloses a microbial composition, microbial preparation and method for sandy soil improvement, and relates to the technical field of microbes. The microbial composition for sandy soil improvement contains bacillus subtilis, bacillus mojavensis and bacillus amyloliquefaciens. The microbial composition is applied to a sandy area, not only can make grains of sand agglomerated well andbe kept in a relatively stable state, but also can effectively improve the physicochemical properties of the soil, can be widely applied to sandy soil restoration, and is conductive to achieving the sustainable development of the sandy area.

The invention provides Trichoderma longibrachiatum with a preservation number of CGMCC No.9727. The invention further provides a compound microorganism microbial agent. The compound microorganism microbial agent comprises the following active ingredients: Trichoderma longibrachiatum, Bacillus cereus and Bacillus mojavensis. The invention further provides application of Trichoderma longibrachiatum or the compound microorganism microbial agent in prevention and treatment of cotton verticillium wilt. According to the technical scheme, the cotton verticillium wilt can be effectively prevented and controlled.

The invention discloses a pear blight antagonistic bacterium LKYHC-2 and belongs to bacillus mojavensis. The length of 16S rDNA nucleotide sequence of the antagonistic bacterium is 1390bp, the antagonistic bacterium is preserved in China General Microbiological Culture Collection Center on November 29th 2018, and a preservation number of the bacterium is CGMCC No.16838. A bacterial colony of the antagonistic bacterium LKYHC-2 is milk white on an LB solid culture medium, the middle of the bacterial colony is concave, the concave is not obvious, the bacterial colony is not transparent, the bacterial colony has wrinkles, the bacterial colony can generate red pigment of gram positive bacterium, and the gram positive bacterium is in a rod shape and has spores. The antagonistic bacterium LKYHC-2can be applied to preventing and treating pear blight, and a relative inhibition rate of the antagonistic bacterium to hypha of pathogenic bacteria of the pear blight is 88.84%; after the antagonistic bacterium LKYHC-2 is applied to fields to prevent and treat the pear blight, a scab cure rate is 66.67%, and a scab relapsing rate is 13.33%. The antagonistic bacterium is safe to environment, humanand livestock and solves the problem of drug resistance generated by using chemical agents to prevent and treat the pear blight for a long time.

A biological material is disclosed for exerting antagonism against vegetable pathogens, which contains Bacillus mojavensis R3B mutant strain deposited under # NCAIM (P) B 001389 according to the Budapest Treaty. The biological material according to the invention is an effective antagonist against the pathogens of vegetables, preferably tomato, pepper, lettuce and / or cabbage, in particular against the pathogens selected from the group of Xanthomonas vesicatoria, Pseudomonas syringae and Clavibacter michiganensis vegetable pathogen bacteria and Pythium debaryanum, Phytophthora infestans, Alternaria alternata and Fusarium oxysporum vegetable pathogen fungi. Furthermore, the invention relates to a composition comprising the biological material according to the invention and optionally a copper-containing pesticide and a process for controlling vegetable pathogens, furthermore use of the biological material or composition according to the invention for the protection of vegetables, preferably tomato, pepper, lettuce and / or cabbage.

The invention relates to the technical field of environmental microorganisms, in particular to an ecological toilet treatment strain and an application thereof. The invention provides bacillus mojavensis BM-Y3. A preservation number of the bacillus mojavensis BM-Y3 is CCTCC NO: M 2019815. The decomposition rate of organic matters in the excrement can be effectively increased by the bacillus mojavensis BM-Y3; the strain can be used for reduction and stabilization treatment of excrement in the ecological toilet treatment process, and has high application value.

The invention provides a lipase derived from Bacillus mojavensis WBRD1.10062302, CGMCC4961. The lipase provided by the invention has better tolerance on multiple organic solvents, such as methanol, ethanol, acetone, acetonitrile, dimethyl sulfoxide, normal hexane, and the like, thereby having application value in multiple industrial fields of biological diesel oil, medical industry, food, oil chemical industry, environmental protection, washing agents, spinning, papermaking, and the like.

The invention discloses a compound microorganism preparation for preventing and treating powdery mildew and a preparation method thereof, and particularly discloses a compound microorganism preparation for preventing and treating powdery mildew of cucumbers and a preparation method thereof. Raw materials of the compound microorganism preparation include Streptomyces plicatus fermentation liquor, Streptomyces phaeofaciensMaeda fermentation liquor, Bacillus mojavensis fermentation liquor and Lysinibacillus sphaericus fermentation liquor. The strains of the compound microorganism preparation can generate multiple antibiotics such as pheofacin, amicetin, amicecin B and plicacetin and perform a super-strong effect on pathogenic bacteria of powdery mildew of cucumbers. The four strains used in the compound microorganism preparation can be directly separated from soil, have a rhizosphere growth promoting effect, can reproduce and grow on the surfaces of crops or in plants or in soil, and also can directionally secrete some secondary metabolites towards the rhizosphere of plants, improve nutrient absorption of plants, simulate plant growth and perform a fertilizer effect. The compound microorganism preparation is safe to human and livestock and environmentally friendly.

The invention provides a lipase derived from Bacillus mojavensis WBRD1.10062302, CGMCC4961. The lipase provided by the invention has better tolerance on multiple organic solvents, such as methanol, ethanol, acetone, acetonitrile, dimethyl sulfoxide, normal hexane, and the like, thereby having application value in multiple industrial fields of biological diesel oil, medical industry, food, oil chemical industry, environmental protection, washing agents, spinning, papermaking, and the like.