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Bacillus mojavensis QLY002 strain, microbial inoculum, preparation method of microbial inoculum and use of Bacillus mojavensis QLY002 strain and microbial inoculum

A microbial inoculum, the technology of Bacillus wei wei, applied in microorganism-based methods, biochemical equipment and methods, botanical equipment and methods, etc., can solve the problems of poor environmental protection, easy to produce drug resistance and safety, etc.

Inactive Publication Date: 2014-11-19
GANSU AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a Mojave bacillus Bacillus mojavensis QLY002 bacterial strain, utilize described endophytic bacterium Mojave spore Bacillus broad-spectrum bactericidal activity and growth-promoting ability to prepare endophytic bacteria Mojave Bacillus microbial inoculum with good environmental protection, not easy to produce drug resistance and good safety

Method used

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  • Bacillus mojavensis QLY002 strain, microbial inoculum, preparation method of microbial inoculum and use of Bacillus mojavensis QLY002 strain and microbial inoculum
  • Bacillus mojavensis QLY002 strain, microbial inoculum, preparation method of microbial inoculum and use of Bacillus mojavensis QLY002 strain and microbial inoculum
  • Bacillus mojavensis QLY002 strain, microbial inoculum, preparation method of microbial inoculum and use of Bacillus mojavensis QLY002 strain and microbial inoculum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1 Acquisition and identification of Bacillus mojavensis QLY002 (CGMCC No.8905)

[0052] 1. Acquisition of Bacillus mojavensis QLY002 (CGMCC No.8905)

[0053] The present invention extracts from the endophytic bacteria of Polygonum viviparum (Polygonum viviparum), Stipa purpurca Griseb (Stipa purpurca Griseb), Kobreasia Capillifolia (Kobreasia Capillifolia), Gentiana macrophy pall and Anaphalis Lactea Maxin, etc. , using the confrontation culture method, that is, the pathogenic bacteria are inserted into the center of the petri dish, and the endophytic bacteria are inoculated equidistantly around 2.5 cm. After 5-7 days of culture, the diameter of the colony is measured by the cross method, and the antibacterial rate is calculated. Based on the antibacterial rate, a screening is obtained. An endophytic bacterium with antibacterial and growth-promoting effects on potato pathogenic bacteria: Mojave bacillus strain, the Latin name of which is Bacillus mojavensis QLY0...

Embodiment 2

[0061] Example 2 Preparation of Bacillus mojavensis QLY002 (CGMCC No.8905) microbial agent

[0062] The bioactive components and weight percent content of the microbial agent of the present invention include: Mojave bacillus CGMCC No. 89055% and the balance of the culture medium.

[0063] The components and contents of the culture medium include: 10g of ammonium chloride, 14g of corn flour, 180g of potatoes, pH value 7.2, and 1000mL of distilled / deionized water.

[0064] The preparation method of the microbial agent is as follows: inoculate the Bacillus Mojave CGMCC No.8905 in the medium in proportion, and carry out liquid fermentation under the conditions of the temperature of 26°C, the rotation speed of 150r / min and the dissolved oxygen of 50%. After 32 hours, the obtained fermentation broth is the microbial agent.

[0065] It has been determined that the number of live bacteria of Bacillus Mojave CGMCC No.8905 in the above fermentation broth can reach 4.12×10 10 CFU / mL. ...

Embodiment 3

[0066] Example 3 Preparation of Bacillus mojavensis QLY002 (CGMCC No.8905) microbial agent

[0067] The bioactive components and weight percent content of the microbial agent of the present invention include: Mojave bacillus CGMCC No. 89058% and the balance of the culture medium.

[0068] The components and content of the medium include: 14.25g ammonium chloride, 19g corn flour, 230g potatoes, pH 7.7, 1000mL distilled / deionized water.

[0069] The preparation method of the microbial agent is as follows: inoculate the Bacillus Mojave CGMCC No.8905 in the medium in proportion, and carry out liquid fermentation under the conditions of the temperature of 28°C, the rotation speed of 180r / min and the dissolved oxygen amount of 60%. After 36 hours, the fermented liquid obtained is the microbial agent.

[0070] It has been determined that the number of live bacteria of Bacillus Mojave CGMCC No.8905 in the above fermentation broth can reach 1.59×10 11 CFU / mL.

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Abstract

The invention provides a Bacillus mojavensis QLY002 strain. The Bacillus mojavensis QLY002 strain has a preservation number of CGMCC No. 8905. The invention also provides a microbial inoculum of the Bacillus mojavensis QLY002 strain and its preparation method and use in potato pathogen inhibition and potato growth promotion. The Bacillus mojavensis QLY002 strain and the microbial inoculum can be used as microbe pesticides and fertilizer, can inhibit Phoma foveata, Alternaria solani, Fusarium oxysporum and Colletotrichum coccodes, have an antibacterial rate more than 61%, have potato growth promotion effects, can be used as potato special-purpose microbial pesticide and microbial fertilizer, solve the problem that the existing chemical pesticide has poor environmental friendliness, easily causes pesticide resistance and has poor safety, and have the advantages of good environmental friendliness, no pesticide resistance and good safety.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides and microbial fertilizers, and in particular relates to a strain of Bacillus mojavensis QLY002 and its microbial agent and application. Background technique [0002] With the large and frequent use of chemical pesticides, the problems of environmental pollution, pesticide residues and increased resistance are becoming more and more serious, and the biological control of plant diseases has become a research hotspot, especially the research on plant endophytes as antagonistic bacteria. attention of many scholars. Plant endophytic bacteria refer to bacteria that live in the roots, stems, leaves, flowers, fruits and seeds of plants, but do not cause any substantial damage to plants. They have a variety of biological functions, especially antibacterial functions. The strong support of bacterial resources. Studies have found that endophytic bacteria can promote the growth of plants, and t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01N63/00A01P3/00A01P21/00C05F11/08C12R1/07
Inventor 杨成德陈秀蓉薛莉畅涛李振东
Owner GANSU AGRI UNIV
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