14 results about "Phoma" patented technology

The invention relates to a microbial preparation containing arbuscular mycorrhizal fungi and application thereof, and belongs to the technical field of plant cultivation. According to the application disclosed by the invention, it is found that all of the glomus amarae, the rhizopus intro-rhizosporium, the glomus mosseae and the cyst macrosporium in the arbuscular mycorrhizal fungi can promote the growth of plants under the condition of double pollution of molybdenum and micro-plastics, and the plant height and the grain yield of the plants are effectively improved. Further experiments find that under the same condition, the effect is better than that of applying a single arbuscular mycorrhizal fungus inoculant when the near-phoma globosum, the rhizosporium intraradices and the glomus mosseae or the mixed inoculant of the near-phoma globosum, the glomus mosseae and the cyst macrosporium is applied. Furthermore, after beneficial bacteria and auxiliary materials are added into the mixed bacterial agent, the mixed bacterial agent has a better promoting effect on plant growth under the condition of double pollution of molybdenum and micro-plastics. The microbial preparation provided by the invention can reduce poison of microplastics and molybdenum to plants, so that the plants can normally grow under dual pollution.

The present invention relates to methods for controlling or preventing infestation of vegetable, tomato and potato plants by phytopathogenic microorganisms selected from Sphaerotheca fuliginea, Leveillula taurica, Sclerotinia sclerotiorum, Cercospora, Fusarium oxysporum, Fusarium solani, Helminthosporium solani, Phoma tuberosa, Rhizoctonia solani, Phytophthora infestans, Verticillium dahlia, Didymella bryoniae, Botrytis cinerea and Alternaria solani, comprising applying to a crop of plants, the locus thereof, or propagation material thereof, a compound according to formula (I) wherein R1, R2, R3, R4, R5, Y, A, B are as defined herein.

The present disclosure provides a method for reducing methane emissions from a ruminant comprising administering to the ruminant a composition comprising a fungal strain, biomass from the fungal strain, a culture supernatant from the fungal strain wherein the fungal strain comprises at least one vanadium-dependent haloperoxidase (VHPO) with an amino acid sequence having at least 80% identity with an amino acid sequence selected from the group consisting of SEQ ID NOs: 3-100 and a combination thereof. The present disclosure also provides a method for reducing methane emissions with a fungal strain belonging to a genus selected from the group consisting of Alternaria, Ascomycota, Bipolaris, Cadophora, Cladorrhinum, Clohesyomyces, Darksidea, Didymella, Dothideomycetes, Drechslera, Exserohilum, Epicoccum, Exophiala, Gaeumannomyces, Gliomastix, Helotiales, Keissleriella, Laburnicola, Lachnum, Leptodontidium, Magnaporthiopsis, Mollisia, Neocamarosporium, Oidiodendron, Ophiosphaerella, Penicillium, Phaeosphaeria, Phialophora, Phoma, Pleosporales, Poaceascoma, Septoriella, Slopeiomyces, Sordariales, Trametes, Trematosphaeria, Trichoderma, and Tricladium.

The application discloses a gonythecium specific molecular detection gene, primer, method and application, and belongs to the technical field of molecular detection. In view of the problems of long time consumption, low accuracy and lack of specific molecular markers in traditional detection methods of the gonythecium specific molecular detection gene, the nucleotide sequence shown in SEQ ID NO:1 is provided as a specific molecular detection target. The sequence lays a foundation for designing high-specificity primers, supports the establishment of a detection system based on conventional PCR and real-time fluorescent quantitative PCR, and the minimum detection concentration reaches 0.5 fg / μL. The technology is mainly used for rapid qualitative and quantitative detection of gonythecium in ginseng plants, seeds and soil, early pathogen monitoring is realized, disease early warning is realized, and technical support is provided for safe production of ginseng.

The application discloses a kind of hydrazine compounds with eugenol as lead and its preparation method and application, and the biological activity test result shows that all compounds are excellent, broad-spectrum antibacterial activity to the plant pathogenic fungi tested.The inhibition effect of most target compounds on plant pathogenic fungi is better than that of positive control rynaxapyr, and the antibacterial effect of compound 11 on rhizoctonia solani, fusarium graminearum, alternaria solani, phoma exigua var. horii and phoma sorghicola is the best, and the EC 50 Values are 0.258 μg / mL, 0.679 μg / mL, 0.424 μg / mL, 0.384 μg / mL and 0.975 μg / mL respectively; for the gossypium gossypium, the antibacterial effect of compound 12 is the best, and the EC 50 Value is 0.989 μg / mL; followed by compound 11, and the EC 50 Value is 1.248 μg / mL.The hydrazine compounds prepared with eugenol as lead have excellent, broad-spectrum and high-efficiency anti-plant pathogenic microorganism activity, and can be used for preventing and treating plant diseases caused by plant pathogenic microorganisms.

The invention discloses a primer group, a kit and a method for identifying the specialized form of poplar brown disc dispora, and relates to the technical field of biological detection, strain genome DNA is used as a template, the primer group is used for multiple PCR amplification reaction, and the primer group can specifically amplify different alleles of EF-1a genes linked with the specialized form of poplar brown disc dispora, so that the specialized form of poplar brown disc dispora can be identified, and the specialized form of poplar brown disc dispora can be identified. Carrying out electrophoresis detection on the PCR amplification product, and judging the strain specialized type according to the fragment size of the amplification product: if a 500bp DNA band appears, judging the strain specialized type as a single bud tube specialized type; and if a 300bp DNA strip appears, determining that the specific type of the multi-bud tube is the specific type of the multi-bud tube. The primer group comprises a single bud tube specialized type specific upstream primer MOE-F1, a multi-bud tube specialized type specific upstream primer MUE-F2 and a universal downstream primer MBE-R. The primer group is characterized in that the primer group comprises a single bud tube specialized type specific upstream primer MOE-F1 and a multi-bud tube specialized type specific upstream primer MUE-F2; the invention provides the first molecular identification method for the specialized type of the poplar brown tray dispora, and the method has the advantages of high specificity, high sensitivity and simplicity and convenience in operation.

The morphological characteristics of Bacillus velezensis CYFSD120 are as follows: after the Bacillus velezensis CYFSD120 is cultured on an NA solid culture medium for 12 hours, a bacterial colony is round, milky white and opaque, the surface of the bacterial colony is wrinkled and raised, the bacterial colony is matte, the edge of the bacterial colony is irregular, and cells of the bacterial colony are rod-shaped. The compound has excellent bacteriostatic activity on nigrospora sphaerica and phoma lingam, the hypha growth inhibition rate on a nigrospora sphaerica HB strain reaches 68.3%, the inhibition rate on a phoma lingam ZY13 strain reaches 70.8%, and the compound is environmentally friendly, has no drug resistance risk, can effectively prevent and treat plant diseases caused by the two pathogenic bacteria, provides efficient microbial resources for agricultural green prevention and control, and has broad application prospects. The method is suitable for being popularized and applied to crops such as tobacco, tomatoes and oilseed rapes.

The present disclosure provides a method for reducing methane emissions from a ruminant comprising administering to the ruminant a composition comprising a fungal strain, biomass from the fungal strain, a culture supernatant from the fungal strain wherein the fungal strain comprises at least one vanadium-dependent haloperoxidase (VHPO) with an amino acid sequence having at least 80% identity with an amino acid sequence selected from the group consisting of SEQ ID NOs: 3-100 and a combination thereof. The present disclosure also provides a method for reducing methane emissions with a fungal strain belonging to a genus selected from the group consisting of Alternaria, Ascomycota, Bipolaris, Cadophora, Cladorrhinum, Clohesyomyces, Darksidea, Didymella, Dothideomycetes, Drechslera, Exserohilum, Epicoccum, Exophiala, Gaeumannomyces, Gliomastix, Helotiales, Keissleriella, Laburnicola, Lachnum, Leptodontidium, Magnaporthiopsis, Mollisia, Neocamarosporium, Oidiodendron, Ophiosphaerella, Penicillium, Phaeosphaeria, Phialophora, Phoma, Pleosporales, Poaceascoma, Septoriella, Slopeiomyces, Sordariales, Trametes, Trematosphaeria, Trichoderma, and Tricladium.

The present application relates to the technical field of biocontrol bacteria, and particularly relates to a bacillus siamensis (Bacillus siamensis) ARF-SR1, a biocontrol bacterium and application thereof. Bacillus siamensis The present application provides the preservation number of the bacillus siamensis ARF-SR1 as CGMCC No.33715. The bacillus siamensis ARF-SR1 provided by the present application is a biocontrol strain isolated from a healthy alfalfa field in Ningxia, has a significant inhibitory effect on alfalfa root rot pathogenic bacteria (especially Fusarium solani, Fusarium oxysporum and Phoma sp.), an antagonistic rate of 57.71-68.86%, can reduce the incidence of alfalfa root rot, enriches the biocontrol bacteria resource library, and is suitable for green control of alfalfa root rot.

The invention discloses a 1-arylmethyl-3-aryl tetrahydropyrimidine-2 (1H)-ketone compound as well as a preparation method and application thereof, the compound has a structure as shown in a general formula (VI), and R1 and R2 are substituent groups such as hydrogen, alkyl, alkoxy, halogen, cyano or tert-butyl. The compounds take CO2 as a carbonyl source, the preparation method is simple, the raw materials are easy to obtain, and the series of tetrahydropyrimidone compounds have a good inhibition effect on the activity of crop germs, particularly have a remarkable inhibition effect on the activity of germs such as phytophthora germs, rhizoctonia solani, valsa ceratosperma and sclerotinia sclerotiorum, and can be used for preparing agricultural antibacterial drugs.

The invention relates to the technical field of biocontrol bacteria, in particular to bacillus siamensis ARF-SR1, a biocontrol bacterial agent and application of the bacillus siamensis ARF-SR1 and the biocontrol bacterial agent. The preservation number of the bacillus siamensis ARF-SR1 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No. 33715. The bacillus siamensis ARF-SR1 provided by the invention is a biocontrol strain separated from a healthy alfalfa field in Ningxia, has a remarkable inhibition effect on alfalfa root rot pathogenic bacteria (particularly fusarium solani, fusarium acuminatum and phomopsis sp.), has an antagonism rate of 57.71-68.86%, can reduce the incidence rate of alfalfa root rot, enriches a biocontrol bacterium resource library, and has a good application prospect. The composition is suitable for green control of alfalfa root rot.

The invention discloses a composite microbial inoculant for preventing and treating rice bakanae disease as well as a preparation method and application of the composite microbial inoculant, the composite microbial inoculant is composed of a fungal hypha suspension of plant endophytic fungi phomopsis sp. And a bacterial suspension formed by mixing pseudomonas otitis media BF72 and bacillus subtilis BF86 according to an equal proportion; the amount (dry weight) of fungal hyphae in the composite bacterial agent is 0.01 g / 5ml, and the effective viable count of the composite bacterial suspension is 1 * 10 < 8 >-10 < 9 > CFU. The composite microbial agent screened, combined and constructed by the invention has an obvious inhibition effect on the occurrence of the rice bakanae disease, improves the growth state of diseased rice, reduces the abundance of pathogenic bacteria in the rice, and improves the POD, SOD and PAL activities of the overground part and the underground part of the rice, so that the resistance of the rice to the pathogenic bacteria is enhanced, and the control effect of the composite microbial agent is superior to that of a chemical agent. The method is low in operation cost, has universality and is easy for large-scale production.

The invention discloses a microbial agent composition beneficial to foliage spraying of dendrobium nobile, and relates to the technical field of agricultural microbial application. Dendrobium nobile endophytic phyllosticta is adopted as a core bacterium, bacillus amyloliquefaciens and streptomyces jingyangensis are compounded, and dendrobium nobile stem extract and jasmonic acid methyl ester are matched to serve as dual inducers; the preparation method comprises the following steps: constructing an integrated auxiliary agent system consisting of a chitosan-nano-selenium compound, erythritol and vitamin C; designing a special nutritional auxiliary material containing chelated calcium and magnesium, dendrobium nobile leaf superfine powder and betaine; the finished product is prepared by the processes of independently propagating strains, mixing the strains in sequence, compounding additives in stages and lagging in addition of nutritional auxiliary materials. The composition provided by the invention can improve the colonization rate of the strain on the lignified leaves of the dendrobium nobile, enhance the nutrient penetration and absorption capability, and directionally promote the accumulation of dendrobine, also gives consideration to the effects of growth promotion and disease resistance, and meets the medicinal cultivation requirements of the dendrobium nobile.

This application provides a specific primer pair, rapid detection method, and application for detecting or identifying *Heterophyllum heterophyllum*. The primer pair is F3 / R3, with the nucleotide sequence of the upstream primer F3 shown in SEQ ID No:1 and the nucleotide sequence of the downstream primer R3 shown in SEQ ID No:2. This application designed the primer pair F3 / R3 by comparing the ITS sequences of *Heterophyllum heterophyllum* with those of other species in the genus *Heterophyllum*, establishing a conventional PCR and qPCR detection system. Identification can be completed within 1-2 days, exhibiting high specificity and sensitivity. It can effectively distinguish *Heterophyllum heterophyllum* from closely related species and other alfalfa root rot pathogens, and is suitable for rapid detection of diseased plants, soil, and seed samples in the field.