6 results about "Phoma" patented technology

The invention discloses a primer group, a kit and a method for identifying the specialized form of poplar brown disc dispora, and relates to the technical field of biological detection, strain genome DNA is used as a template, the primer group is used for multiple PCR amplification reaction, and the primer group can specifically amplify different alleles of EF-1a genes linked with the specialized form of poplar brown disc dispora, so that the specialized form of poplar brown disc dispora can be identified, and the specialized form of poplar brown disc dispora can be identified. Carrying out electrophoresis detection on the PCR amplification product, and judging the strain specialized type according to the fragment size of the amplification product: if a 500bp DNA band appears, judging the strain specialized type as a single bud tube specialized type; and if a 300bp DNA strip appears, determining that the specific type of the multi-bud tube is the specific type of the multi-bud tube. The primer group comprises a single bud tube specialized type specific upstream primer MOE-F1, a multi-bud tube specialized type specific upstream primer MUE-F2 and a universal downstream primer MBE-R. The primer group is characterized in that the primer group comprises a single bud tube specialized type specific upstream primer MOE-F1 and a multi-bud tube specialized type specific upstream primer MUE-F2; the invention provides the first molecular identification method for the specialized type of the poplar brown tray dispora, and the method has the advantages of high specificity, high sensitivity and simplicity and convenience in operation.

The morphological characteristics of Bacillus velezensis CYFSD120 are as follows: after the Bacillus velezensis CYFSD120 is cultured on an NA solid culture medium for 12 hours, a bacterial colony is round, milky white and opaque, the surface of the bacterial colony is wrinkled and raised, the bacterial colony is matte, the edge of the bacterial colony is irregular, and cells of the bacterial colony are rod-shaped. The compound has excellent bacteriostatic activity on nigrospora sphaerica and phoma lingam, the hypha growth inhibition rate on a nigrospora sphaerica HB strain reaches 68.3%, the inhibition rate on a phoma lingam ZY13 strain reaches 70.8%, and the compound is environmentally friendly, has no drug resistance risk, can effectively prevent and treat plant diseases caused by the two pathogenic bacteria, provides efficient microbial resources for agricultural green prevention and control, and has broad application prospects. The method is suitable for being popularized and applied to crops such as tobacco, tomatoes and oilseed rapes.

The present application relates to the technical field of biocontrol bacteria, and particularly relates to a bacillus siamensis (Bacillus siamensis) ARF-SR1, a biocontrol bacterium and application thereof. Bacillus siamensis The present application provides the preservation number of the bacillus siamensis ARF-SR1 as CGMCC No.33715. The bacillus siamensis ARF-SR1 provided by the present application is a biocontrol strain isolated from a healthy alfalfa field in Ningxia, has a significant inhibitory effect on alfalfa root rot pathogenic bacteria (especially Fusarium solani, Fusarium oxysporum and Phoma sp.), an antagonistic rate of 57.71-68.86%, can reduce the incidence of alfalfa root rot, enriches the biocontrol bacteria resource library, and is suitable for green control of alfalfa root rot.

The invention discloses a 1-arylmethyl-3-aryl tetrahydropyrimidine-2 (1H)-ketone compound as well as a preparation method and application thereof, the compound has a structure as shown in a general formula (VI), and R1 and R2 are substituent groups such as hydrogen, alkyl, alkoxy, halogen, cyano or tert-butyl. The compounds take CO2 as a carbonyl source, the preparation method is simple, the raw materials are easy to obtain, and the series of tetrahydropyrimidone compounds have a good inhibition effect on the activity of crop germs, particularly have a remarkable inhibition effect on the activity of germs such as phytophthora germs, rhizoctonia solani, valsa ceratosperma and sclerotinia sclerotiorum, and can be used for preparing agricultural antibacterial drugs.

The invention discloses a microbial agent composition beneficial to foliage spraying of dendrobium nobile, and relates to the technical field of agricultural microbial application. Dendrobium nobile endophytic phyllosticta is adopted as a core bacterium, bacillus amyloliquefaciens and streptomyces jingyangensis are compounded, and dendrobium nobile stem extract and jasmonic acid methyl ester are matched to serve as dual inducers; the preparation method comprises the following steps: constructing an integrated auxiliary agent system consisting of a chitosan-nano-selenium compound, erythritol and vitamin C; designing a special nutritional auxiliary material containing chelated calcium and magnesium, dendrobium nobile leaf superfine powder and betaine; the finished product is prepared by the processes of independently propagating strains, mixing the strains in sequence, compounding additives in stages and lagging in addition of nutritional auxiliary materials. The composition provided by the invention can improve the colonization rate of the strain on the lignified leaves of the dendrobium nobile, enhance the nutrient penetration and absorption capability, and directionally promote the accumulation of dendrobine, also gives consideration to the effects of growth promotion and disease resistance, and meets the medicinal cultivation requirements of the dendrobium nobile.

This application provides a specific primer pair, rapid detection method, and application for detecting or identifying *Heterophyllum heterophyllum*. The primer pair is F3 / R3, with the nucleotide sequence of the upstream primer F3 shown in SEQ ID No:1 and the nucleotide sequence of the downstream primer R3 shown in SEQ ID No:2. This application designed the primer pair F3 / R3 by comparing the ITS sequences of *Heterophyllum heterophyllum* with those of other species in the genus *Heterophyllum*, establishing a conventional PCR and qPCR detection system. Identification can be completed within 1-2 days, exhibiting high specificity and sensitivity. It can effectively distinguish *Heterophyllum heterophyllum* from closely related species and other alfalfa root rot pathogens, and is suitable for rapid detection of diseased plants, soil, and seed samples in the field.