34 results about "Phoma" patented technology

The invention discloses a lilium regale gene Lr14-3-3 with antifungal activity. The gene Lr14-3-3 has the nucleotide sequence as shown in SEQ ID NO:1 and encodes protein 14-3-3. A relevant technology of functional genomics proves that the gene Lr14-3-3 has a function of improving the plant antifungal activity. The antifungal gene Lr14-3-3 is constructed to a plant expression vector and is transferred into tobacco to perform overexpression; the transgenic tobacco plant has strong in vitro antifungal activity; and the transgenic tobacco expressing the Lr14-3-3 has obvious inhibition effects on the growth of botryosphaeria dothidea, phomopsis fungi, fusarium oxysporum and alternaria.

The invention provides a Jiang chuan mycin for preventing and controlling plant diseases and a pesticide compound of the Jiang chuan mycin, which is used for disinfecting and / or resisting crop diseases. The pesticide compound has excellent effects for resisting plant pathogens such as phoma asparagi, pellicularia gramineum, vanturia pirina, saccharomycete, botrytis cinerea, helmintosporium maydis, pyricuraria oryzae, colletotrichum lagenarium and / or alternaria mali Roberts.

The invention discloses a pathogen of Amomum villosum seedling blight, specifically Phoam sp.ZOLaiwu, which is preserved in China Center for Type Culture Collection (CCTCC), has a preservation numberof CCTCC NO: M2020287 and a preservation date of July 21, 2020, and is taxonomically named as named as Phoma. According to the invention, the pathogen Phoam sp.ZOLaiwu of Amomum villosum seedling blight is beneficial to the prevention and control of seedling withering in Amomum villosum seedling stage, improves the survival rate of the Amomum villosum seedlings, further studies on the prevention and control of Amomum villosum seedling blight, and then improves the yield of Amomum villosum.

The invention discloses a method for identifying pseudo lysogenic phages SNJ1 of halophilic archaea. The method comprises the following steps: at first, applying the halophilic archaea to be detected to a solid culture medium for culturing for 5-7 days to form single colony; then, inoculating the single colony to a dual-layer flat plate with indicator bacteria in an upper layer, and culturing at constant temperature for 2 days; finally, observing whether the phages are formed on the dual-layer flat plate. According to the method, the phages can be identified without enrichment culture of the single colony and separation of the phages from host cells, so that the identification cycle is shortened, the identification process is simplified, and the cost is reduced.

The invention discloses diphenyl ether phenol as well as a preparation method and application thereof. The diphenyl ether phenol is characterized in that the molecular formula of the diphenyl ether phenol is C15H16O5, and the structural formula of the diphenyl ether phenol is shown as a formula I, the preparation method of the diphenyl ether phenol comprises the following steps: (1) carrying out strain culture on phoma sp. L28 in a strain culture medium; and (2) carrying out fermentation culture on the strain obtained in the step (1) in a fermentation culture medium, then extracting with an organic solvent, and carrying out vacuum concentration to obtain the diphenyl ether phenol. According to the present invention, the obtained diphenyl ether phenol has strong inhibition effects on banana colletotrichum gloeosporioides, tomato fusarium oxysporum and O78 serotype Escherichia coli, and can be used as the related microbial agricultural bactericide or veterinary drug preparation.

The invention provides a Jiang chuan mycin for preventing and controlling plant diseases and a pesticide compound of the Jiang chuan mycin, which is used for disinfecting and / or resisting crop diseases. The pesticide compound has excellent effects for resisting plant pathogens such as phoma asparagi, pellicularia gramineum, vanturia pirina, saccharomycete, botrytis cinerea, helmintosporium maydis, pyricuraria oryzae, colletotrichum lagenarium and / or alternaria mali Roberts.

The invention discloses a method for preparing the anti-tumor compound Eupenifeldin and its special bacterial strain. The special bacterial strain for preparing the anti-tumor compound Eupenifeldin provided by the present invention is specifically Phoma sp. XZ068, and its preservation number in the General Microbiology Center of China Committee for the Collection of Microorganisms is CGMCC No. 10481. Experiments have proved that the present invention utilizes Phoma sp. (Phoma sp.) XZ068CGMCC No.10481 to prepare the method for the antitumor active compound Eupenifeldin, and the yield is high (the crude extract of 10.3g ethyl acetate obtains 400mg pure compound Eupenifeldin through separation and purification, The purity is more than 99%, and the yield is 3.9%), and the method of the invention is simple to operate, low in cost, time-saving and labor-saving.

The invention relates to a decahydronaphthalene compound and an application thereof. According to the invention, the decahydronaphthalene compound is separated and extracted from a fermentation liquidof a symbiotic and endophytic fungus Phoma sp. of Sinularia sandensis, wherein the decahydronaphthalene compound has T-cell subtype regulation activity, is low in cytotoxicity and can be used for preparing immunomodulatory drugs. The invention provides a lead compound for developing novel immunoregulatory drugs, opens up a new way for deep research and development of the novel immunoregulatory drugs, and facilitates development and utilization of marine medicinal resources.

The invention discloses a lilium regale germin-like protein gene LrGLP2 with antifungal activity. The gene LrGLP2 has the nucleotide sequence as shown in SEQ ID:1 and encodes germin-like protein. A relevant technology of functional genomics proves that the gene LrGLP2 has a function of improving the plant antifungal activity. The antifungal gene LrGLP2 is constructed to a plant expression vector and is transferred into tobacco to perform overexpression; and the transgenic tobacco plant has strong in vitro antifungal activity. The LrGLP2 transgenic tobacco protein has different degrees of inhibition effects on the growth of botryosphaeria dothidea, phomopsis fungi, fusarium oxysporum and botrytis cinerea.

The invention discloses a bacillus polymyxa strain and application thereof. The Bacillus polymyxa strain of the present invention, the Bacillus polymyxa strain is PBSZ123, the preserved name is Bacillus polymyxa (Paenibacillus polymyxa), and it is preserved in the General Microorganism Center (CGMCC) of China Microorganism Culture Preservation Management Committee, the depository unit The address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. Date of deposit: August 14, 2019; Collection number: CGMCC No.18386. The bacillus polymyxa of the present invention is isolated from the endophyte of the polyphylla, and has a good inhibitory effect on the main pathogenic bacteria of the polyphylla, Botrytis cinerea, Phoma leaf spot, and Polychaete spp.

The invention discloses a method for synthesizing dialkylhydrogenated noppylbenzyl quaternary ammonium salt compounds and the antibacterial application of such compounds. The invention is obtained by directly synthesizing dialkylhydrogenated noppylamine and benzyl halides in one step Dialkylhydrogenated noppyl benzyl quaternary ammonium salt, in an organic solvent, dialkylhydrogenated noppylamine and benzyl halide (chlorine, bromine, iodine) are fed into a stirring bar and equipped with a certain material ratio respectively Condensate in the Erlenmeyer flask of the reflux device, heat to react, the reaction temperature is controlled at 100-150°C, and the reaction time is controlled at 1-3d. After the reaction, the product is filtered, washed, recrystallized and vacuum-dried to obtain 7 kinds of dialkylhydrogenated notylbenzyl quaternary ammonium compounds respectively, and 7 kinds of dialkylhydrogenated notylbenzyl quaternary ammonium Both Phoma citri and Anthracnose citrus had good inhibitory effect. The invention has rich raw materials, simple operation, mild reaction conditions and simple post-purification treatment. Compared with traditional antibacterial agents, this type of antibacterial compound has the advantages of simple structure, safety and non-toxicity, less environmental pollution, etc., and has a good application prospect.

The invention belongs to marine medicines, and in particular relates to a marine fungus derived from Cordica gorgonianus and its application in the preparation of antibacterial medicines. The strain preservation information of the marine fungus Phoma sp. of the present invention: the name of the preservation unit: China Microbial Strain Preservation Management Committee General Microbiology Center; the preservation unit address: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing, Chinese Academy of Sciences Microbiology Institute; date of deposit: April 1, 2017; deposit number: CGMCC No.13881; classification name: Phoma sp.

The present invention provides a pesticide sterilization composite containing difenoconazole and tebuconazole as the main ingredients, wherein, the weight ratio of the difenoconazole and the tebuconazole is 1-50:1-45; the pesticide sterilization composite mainly has remarkable controlling effect on alternaria leaf spot, phoma citricarpa and anthracnose of apple trees.