The invention discloses a reverse osmosis membrane cleaning method, comprising the steps of cleaning the membrane by using alkaline cleaner, acidic cleaner, and sterilizing agent orderly, simultaneously, observing the decrease value of water yield under constant pressure and temperature, the increase value of net operation pressure at constant temperature and constant water yield, or the decrease value of water quality and desalinization ratio of modular system, to determine the occasion for cleaning reverse osmosis membrane. By using the method provided in the invention, the water yield and pressure difference of reverse osmosis membrane can be repaired to a great degree, the desalinization ratio after cleaning can be improved remarkably, and the removing ratios for TOC, CODMn, and UV254 can also be increased, thereby the method in the invention is simple, convenient, and efficient, and suitable for cleaning reverse osmosis membrane in water treatment process and other application field.