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Bacillus methylotrophicus, microorganism bacterium agents and application of bacillus methylotrophicus and microorganism bacterium agents

A methylotrophic and microbial agent technology, applied in the field of agricultural biology, can solve problems such as poor salinity effect

Active Publication Date: 2014-05-21
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that the existing microbial strains have poor salinity removal effect, the present invention provides a methylotrophic bacillus, the preservation number of which is CCTCC NO.M2013500

Method used

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Examples

Experimental program
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Effect test

preparation example Construction

[0021] Wherein, the preparation method of the microbial bacterial agent may include: inoculating methylotrophic Bacillus, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens respectively in the medium for culturing. Wherein, the methylotrophic Bacillus, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens can be cultured in separate culture systems, and the microorganisms obtained from the culture can be mixed in proportion. Preferably, by cultivating in separate culture systems and mixing after culturing, the total number of live bacteria per gram of microbial agent obtained is 2-20×10 7 CFU.

[0022] According to the present invention, the cultivation method of Bacillus subtilis is not particularly limited and known to those skilled in the art, for example, Bacillus subtilis can be cultivated in seed medium (LB medium) to a bacterial density OD 600 The value is 0.6-0.8, to obtain the bacterial liquid, and then inoculate 2-5 parts by...

Embodiment 1

[0036] This example is used to illustrate the methylotrophic bacillus and its properties of the present invention.

[0037] Inoculate the methylotrophic Bacillus (Bacillus methylotrophicus) with the preservation number CCTCC NO.M2013500 into LB medium (containing tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L), and cultivate to the bacterial density OD 600 The value is 0.6-0.8, and the bacterial liquid is obtained.

[0038] NaCl was added to the LB medium to adjust the concentration of NaCl to 220 g / L, and NaOH was added to adjust the pH value to 9.5 to obtain a saline-alkali stress medium.

[0039] Add 3mL of the above bacterial solution into the saline-alkali stress medium to make the initial OD 600 0.1, at 30°C, shake culture at 180 rpm, take samples every 4h to determine the bacterial density in the fermentation broth (OD 600 ). The results showed that the OD of the fermentation broth at 0, 4, 8, 12, 16, 20, and 24 hours 600 They are 0.1, 0.13, 0.16, 0.23, 0.39, 0.55 r...

Embodiment 2

[0049] This embodiment is used to illustrate the microbial inoculant of the present invention and its performance.

[0050] (1) Inoculate 5 parts by weight of Bacillus subtilis bacteria liquid (purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of culture medium (glucose 5g, soybean meal 30g, peptone 2g, distilled water 1000ml, pH7.0-7.2) And the number is ACCC03221, cultivated in the same LB medium as above to the bacterial density OD 600 The value is 0.6-0.8, to obtain the bacterial liquid), cultivated at 37°C, and samples were taken during the cultivation process and observed by direct counting under a microscope until the number of viable bacteria of Bacillus subtilis was 2×10 7 CFU / gram of medium.

[0051] (2) Inoculate 5 parts by weight of Bacillus licheniformis solution (purchased From the China Agricultural Microbiology Culture Collection Center and the number is ACCC02698, cultured in the same LB medium as above to a ba...

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PUM

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Abstract

The invention provides bacillus methylotrophicus with preservation number-CCTCC NO. M2013500. The invention also provides a microorganism bacterium agent. The microorganism bacterium agent contains a culture medium and thallus, wherein the thallus comprise the bacillus methylotrophicus with preservation number-CCTCC NO. M2013500, bacillus subtilis, bacillus lincheniformis and bacillus amyloliquefaciens. The invention also provides an application of the bacillus methylotrophicus and the above microorganism bacterium agents to improvement of saline lands, improvement of plant drought tolerance, prevention of bacterial wilt of pepper and prevention of Citrus anthracnose.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a methylotrophic bacillus, a microbial agent and applications thereof. Background technique [0002] Land salinization is a serious problem affecting agricultural production and ecological environment. About 20% of the arable land and nearly 50% of the irrigated farmland in the world are seriously affected by the high concentration of salinity in the soil. In China, there are about 100 million mu of secondary salinized farmland, accounting for 10% of the total cultivated land. How to really improve and effectively utilize saline-alkali land and improve the economic, social and ecological benefits of agricultural and forestry production is a major concern of modern agriculture and forestry. [0003] In the field of saline-alkali land improvement research at home and abroad, the agricultural improvement measures of "planting rice to improve alkali", the engineering impro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/00A01N63/00A01P3/00C12R1/07C12R1/125C12R1/10C12R1/01C09K101/00
Inventor 阮志勇孔德龙宋金龙赵秉强李燕婷王彦伟刘艳伟王慧敏陈小蓉刘小飞
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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