Bacillus methylotrophicus, microorganism bacterium agents and application of bacillus methylotrophicus and microorganism bacterium agents

A methylotrophic and microbial agent technology, applied in the field of agricultural biology, can solve problems such as poor salinity effect

Active Publication Date: 2014-05-21
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the defect that the existing microbial strains have poor salinity removal effect,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0021] Wherein, the preparation method of the microbial inoculum may include: inoculating Bacillus methylotrophic, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens in a culture medium respectively for cultivation. Wherein, Methylotrophic Bacillus, Bacillus subtilis, Bacillus licheniformis and Bacillus amyloliquefaciens can be cultured in independent culture systems, and the microorganisms obtained from the culture can be mixed in proportion. Preferably, by culturing in independent culture systems and mixing after culturing, the total viable count per gram of microbial inoculum is 2-20×10 7 CFU.

[0022] According to the present invention, the method for culturing Bacillus subtilis is not particularly limited to those known to those skilled in the art, for example, Bacillus subtilis can be first cultured in a seed medium (LB medium) to a bacterial density OD 600 The value is 0.6-0.8 to obtain bacterial liquid, and then inoculate 2-5 parts by weight of ...

Example Embodiment

[0035] Example 1

[0036] This example is used to illustrate the methylotrophic Bacillus of the present invention and its performance.

[0037] Inoculate Bacillus methylotrophicus (Bacillus methylotrophicus) with preservation number CCTCC NO.M2013500 into LB medium (containing tryptone 10g / L, yeast extract 5g / L, NaCl 10g / L), and cultivate to bacterial density OD 600 The value is 0.6-0.8 to obtain bacterial liquid.

[0038] NaCl was added to the LB medium to adjust the concentration of NaCl to 220 g / L, and NaOH was added to adjust the pH to 9.5 to obtain a saline-alkali stress medium.

[0039] 3mL of the above bacterial liquid was added to the saline-alkali stress medium to make the initial OD. 600 be 0.1, at 30 ° C, shaking culture at 180 rpm, sampling every 4h to measure the bacterial density (OD) in the fermentation broth 600 ). The results showed that the OD of the fermentation broth at 0, 4, 8, 12, 16, 20, and 24 hours 600 0.1, 0.13, 0.16, 0.23, 0.39, 0.55, respective...

Example Embodiment

[0048] Example 2

[0049] This example is used to illustrate the microbial inoculum of the present invention and its performance.

[0050] (1) Inoculate 5 parts by weight of Bacillus subtilis bacterial liquid (purchased from China Agricultural Microorganism Culture Collection Center) in 100 parts by weight of culture medium (5 g of glucose, 30 g of soybean meal, 2 g of peptone, 1000 ml of distilled water, pH 7.0-7.2) And the number is ACCC03221, cultivated to bacterial density OD in the same LB medium as above 600 The value is 0.6-0.8 to obtain bacterial liquid), cultivate at 37 °C, take samples during the cultivation process and observe by direct microscope counting method until the number of viable bacteria of Bacillus subtilis is 2 × 10 7 CFU / gram of medium.

[0051] (2) Inoculate 5 parts by weight of Bacillus licheniformis bacterial liquid (purchased) into 100 parts by weight of culture medium (0.45 g of corn steep liquor, 1.50 g of peptone, 1:15 for bean cake flour infu...

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PUM

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Abstract

The invention provides bacillus methylotrophicus with preservation number-CCTCC NO. M2013500. The invention also provides a microorganism bacterium agent. The microorganism bacterium agent contains a culture medium and thallus, wherein the thallus comprise the bacillus methylotrophicus with preservation number-CCTCC NO. M2013500, bacillus subtilis, bacillus lincheniformis and bacillus amyloliquefaciens. The invention also provides an application of the bacillus methylotrophicus and the above microorganism bacterium agents to improvement of saline lands, improvement of plant drought tolerance, prevention of bacterial wilt of pepper and prevention of Citrus anthracnose.

Description

technical field [0001] The invention relates to the field of agricultural biotechnology, in particular to a methylotrophic bacillus, a microbial agent and applications thereof. Background technique [0002] Land salinization is a serious problem affecting agricultural production and ecological environment. About 20% of the arable land and nearly 50% of the irrigated farmland in the world are seriously affected by the high concentration of salinity in the soil. In China, there are about 100 million mu of secondary salinized farmland, accounting for 10% of the total cultivated land. How to really improve and effectively utilize saline-alkali land and improve the economic, social and ecological benefits of agricultural and forestry production is a major concern of modern agriculture and forestry. [0003] In the field of saline-alkali land improvement research at home and abroad, the agricultural improvement measures of "planting rice to improve alkali", the engineering impro...

Claims

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Application Information

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IPC IPC(8): C12N1/20C09K17/00A01N63/00A01P3/00C12R1/07C12R1/125C12R1/10C12R1/01C09K101/00
Inventor 阮志勇孔德龙宋金龙赵秉强李燕婷王彦伟刘艳伟王慧敏陈小蓉刘小飞
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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