The invention discloses a cultivation method of anammox granular sludge, and belongs to the field of water treatment. The cultivation method solves the technical problems that the existing high-efficiency anammox granular sludge is difficult to cultivate, and the cultivation time is long. The method comprises the following steps that 1, aerobic granular sludge and anammox seed sludge are simultaneously inoculated into an EGSB (expanded granular sludge bed) reactor; 2, nitrogenous simulated waste water is supplied to the EGSB reactor, the hydraulic retention time is kept for 12h, and concentrations of ammonia nitrogen and nitrite nitrogen in intake water are gradually increased until successful start when the synchronous removal rate of ammonia nitrogen and nitrite nitrogen is above 80%; and 3, intake water concentrations of ammonia nitrogen and nitrite nitrogen are kept stable, the hydraulic retention time is gradually shortened up to 2.6h when the synchronous removal rate of ammonia nitrogen and nitrite nitrogen is above 80%, and then the cultivation of the anammox granular sludge is completed. With the method, the reactor can be started successfully within about 20 days, and the high-efficiency anammox granular sludge can be obtained within about 89 days.