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Cultivation method of anammox granular sludge

A technology of anaerobic ammonium oxidation and aerobic granular sludge, which is applied in the field of water treatment, can solve the problems of high-efficiency anaerobic ammonium oxidation granular sludge cultivation difficulties and long cultivation time, and achieve accelerated enrichment growth, enrichment cultivation and The effect of increasing the removal rate of nitrogen

Active Publication Date: 2013-04-17
BEIJING UNIV OF CIVIL ENG & ARCHITECTURE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention solves the technical problems of difficult cultivation of high-efficiency anaerobic ammonium oxidation granular sludge and long cultivation time; and provides a cultivation method of anaerobic ammonium oxidation granular sludge

Method used

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  • Cultivation method of anammox granular sludge
  • Cultivation method of anammox granular sludge
  • Cultivation method of anammox granular sludge

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specific Embodiment approach 1

[0014] Specific embodiment one: the cultivation method of anammox granular sludge in the present embodiment is carried out according to the following steps:

[0015] Step 1. Inoculate aerobic granular sludge and anammox seed sludge into the EGSB reactor at the same time. The sum of the volume of aerobic granular sludge and anammox seed sludge accounts for 28%~35% of the total volume of the reactor , the volume ratio of aerobic granular sludge and anammox seed sludge is 1: (2.0-2.3);

[0016] Step 2. Pass nitrogen-containing simulated wastewater with a dissolved oxygen concentration of 0-0.8mg / L and a pH value of 7.5-8.3 into the EGSB reactor at an ascending flow rate of 3.0m / h, operate at 30-35°C, and control HRT is 12h; detect the concentration of ammonia nitrogen in the influent and effluent of the EGSB reactor, keep the hydraulic retention time at 12h, and gradually increase the ammonia nitrogen and nitrite in the nitrogen-containing simulated wastewater when the simultaneo...

specific Embodiment approach 2

[0019] Specific embodiment two: the difference between this embodiment and specific embodiment one is: KHCO in the nitrogen-containing simulated wastewater 3 The concentration is 1000mg / L, KH 2 PO 4 The concentration is 50mg / L, MgSO 4 ·7H 2 The concentration of O is 200mg / L, CaCl 2 2H 2 The concentration of O is 151mg / L, ZnSO 4 ·7H 2 The concentration of O is 430mg / L, CoCl 2 ·6H 2 The concentration of O is 240mg / L, CuSO 4 ·5H 2 O concentration is 250mg / L, H 3 BO 4 The concentration is 14mg / L, MnCl 2 4H 2 The concentration of O is 990mg / L, NaSeO 4 10H 2 The concentration of O is 210mg / L, NiCl·6H 2 The concentration of O is 190mg / L, NaMoO 4 2H 2 The concentration of O is 220 mg / L, the concentration of vitamin B6 hydrochloride is 10 mg / L, the concentration of vitamin B1 hydrochloride is 5 mg / L, the concentration of vitamin B2 is 5 mg / L, and the concentration of niacin is 5 mg / L , the concentration of pantothenic acid is 5mg / L, the concentration of p-aminobenzoi...

specific Embodiment approach 3

[0020] Specific embodiment three: the difference between this embodiment and specific embodiment two is: NH in the nitrogen-containing simulated wastewater described in step one 4 The initial concentration of Cl is 19.5~23.6mg / L, NaNO 2 The initial concentration is 31.3~32.4mg / L. Other steps and parameters are the same as in the second embodiment.

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Abstract

The invention discloses a cultivation method of anammox granular sludge, and belongs to the field of water treatment. The cultivation method solves the technical problems that the existing high-efficiency anammox granular sludge is difficult to cultivate, and the cultivation time is long. The method comprises the following steps that 1, aerobic granular sludge and anammox seed sludge are simultaneously inoculated into an EGSB (expanded granular sludge bed) reactor; 2, nitrogenous simulated waste water is supplied to the EGSB reactor, the hydraulic retention time is kept for 12h, and concentrations of ammonia nitrogen and nitrite nitrogen in intake water are gradually increased until successful start when the synchronous removal rate of ammonia nitrogen and nitrite nitrogen is above 80%; and 3, intake water concentrations of ammonia nitrogen and nitrite nitrogen are kept stable, the hydraulic retention time is gradually shortened up to 2.6h when the synchronous removal rate of ammonia nitrogen and nitrite nitrogen is above 80%, and then the cultivation of the anammox granular sludge is completed. With the method, the reactor can be started successfully within about 20 days, and the high-efficiency anammox granular sludge can be obtained within about 89 days.

Description

technical field [0001] The invention belongs to the field of water treatment; in particular, it relates to a method for cultivating anaerobic ammonium oxidation granular sludge. Background technique [0002] Anammox bacteria ANAMMOX bacteria use nitrite as electron acceptor to oxidize ammonia nitrogen, use inorganic carbon as carbon source, and do not need organic matter as carbon source, so as to achieve the purpose of autotrophic biological denitrification. It can save 60% of the aeration rate and 100% of the organic carbon source, so that the organic matter in the water can be fully utilized to produce energy substances—methane. [0003] At the same time, the growth of anammox bacteria is slow, the generation time is about 11 days, and they are extremely sensitive to the environment. Therefore, the existing anammox reactors generally have long start-up time, difficult control of operating conditions, poor treatment efficiency, and low nitrogen load. question. Granular s...

Claims

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Application Information

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IPC IPC(8): C02F3/28C02F3/34
Inventor 高大文丛岩袁青黄晓丽陶彧任南琪
Owner BEIJING UNIV OF CIVIL ENG & ARCHITECTURE
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