275 results about "Pantothenic acid" patented technology

The present disclosure provides a recombinant yeast host cell having a plurality of native and / or heterologous enzymes that function in an engineered metabolic pathway to convert fructose-6-phosphate to acetyl-coenzyme A. The recombinant yeast host cell can be used, for example, in a process for making acetyl-coenzyme A, acetone and / or isopropanol. The plurality of native and / or heterologous enzymes is activated, upregulated, or overexpressed. The plurality of native and / or heterologous enzymes comprises a phosphoketolase; and optionally an acetate kinase; and / or a phosphotransacetylase. The recombinant yeast host cell comprises at least one of: at least two copies of a heterologous nucleic acid molecule encoding the phosphoketolase; a native and / or heterologous enzyme that functions in an engineered non-oxidative pentose phosphate pathway, wherein the native and / or heterologous enzyme is activated, upregulated, or overexpressed; and / or a native and / or heterologous protein that functions in an engineered metabolic pathway to convert pantothenate into acetyl-coenzyme A, wherein the native and / or heterologous protein is activated, upregulated or overexpressed and comprises at least one of: FEN2 or CAB1.

The invention provides a keto-alcohol acid reductoisomerase mutant and application of the keto-alcohol acid reductoisomerase mutant in construction of genetically engineered bacteria for high yield of D-pantothenic acid. In order to solve the technical problem that in the prior art, the yield of D-pantothenic acid synthesized through microbial fermentation is not high, semi-rational design of enzyme is adopted, and a keto-alcohol acid reductoisomerase mutant with substrate specificity is obtained and applied to construction of genetically engineered bacteria for high yield of D-pantothenic acid. According to the invention, a genetically engineered bacterium with high yield of D-pantothenic acid is constructed by utilizing the keto-alcohol acid reductoisomerase mutant through a CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04%, and compared with an original strain, the valine accumulation amount in obtained fermentation liquor is almost unchanged compared with that of a control, and the yield of D-pantothenic acid is greatly improved. However, the accumulation of branched chain amino acid isoleucine is less, so that the modification of the substrate specificity of the keto-alcohol acid reductoisomerase is achieved.

The invention discloses a serum-free adipogenic differentiation method for porcine adipose precursor cells, and relates to the technical field of mammalian animal cell differentiation.In the adipogenic differentiation process, an induced differentiation culture medium and a maintained differentiation culture medium are sequentially used, and the induced differentiation culture medium is composed of a basic culture medium and cell culture auxiliary factors; the cell culture auxiliary factor is composed of 66nmol / L insulin, 33 [mu] mol / L biotin, 17 [mu] mol / L pantothenic acid, 0.1 [mu] mol / L dexamethasone, 0.25 mmol / L IBMX, 1 [mu] mol / L rosiglitazone, 150 [mu] mol / L sodium oleate and 10 [mu] g / mL transferrin; the maintenance differential medium removes IBMX and rosiglitazone on the basis of inducing the differential medium. The serum-free adipogenic differentiation method for the porcine fat precursor cells, provided by the invention, is good in differentiation effect and high in repeatability.

The invention discloses a fermentation medium for regulating and controlling a clostridium aerovorans target product and a fermentation method of the clostridium aerovorans target product. The medium comprises a basic component and a regulating component, the basic components comprise ammonium chloride, potassium chloride, magnesium sulfate heptahydrate, sodium chloride, monopotassium phosphate, yeast powder, calcium chloride dihydrate, sodium selenite, nickel chloride hexahydrate, biotin, folic acid, pyridoxine hydrochloride, thiamine, riboflavin, nicotinic acid, D-calcium pantothenate, cobalamin, para aminobenzoic acid and lipoic acid; the adjusting component comprises sodium tungstate, ferrous sulfate and sodium molybdate, sodium tungstate and ferrous sulfate have a forward adjusting effect on increasing the yield of acetic acid, ethanol and butanol in the target product, and sodium molybdate has a reverse adjusting effect on the target product. By optimizing the proportion of trace elements in the culture medium, the clostridium metabolism efficiency is improved, synthesis of a target product is regulated and controlled, and the method is particularly suitable for industrial production of acetic acid, ethanol, butanol and the like.

The utility model discloses a calcium pantothenate production and processing crystallization kettle with a wall sticking prevention function, which relates to the technical field of crystallization kettles and comprises a kettle body, a feed port is mounted at the upper part of the kettle body, a discharge port is mounted at the bottom of the kettle body, a stirring piece is mounted in the kettle body, and a cleaning piece is mounted on the stirring piece. According to the utility model, airflow is transmitted by starting the control valve and the air pump, and then is transmitted through the transmission pipe, so that the airflow is transmitted into the exhaust pipe and is exhausted through the exhaust hole, and residual materials scraped by the scraper blade can be removed, so that the residual materials on the inner wall of the kettle body can be removed more effectively; the motor is started to drive the rotating shaft to rotate, then the cleaning assembly fixedly connected with the connecting rod is driven to rotate, and then the cleaning assembly is driven to move leftwards through centrifugal force, so that the scraping plate is driven to move leftwards, the scraping plate is attached to the inner wall of the kettle body, and residual materials on the inner wall of the kettle body can be cleaned.

The invention provides a keto-alcohol acid reductoisomerase mutant and application of the keto-alcohol acid reductoisomerase mutant in construction of genetically engineered bacteria for high yield of D-pantothenic acid. In order to solve the technical problem that in the prior art, the yield of D-pantothenic acid synthesized through microbial fermentation is not high, semi-rational design of enzyme is adopted, and a keto-alcohol acid reductoisomerase mutant with substrate specificity is obtained and applied to construction of genetically engineered bacteria for high yield of D-pantothenic acid. According to the invention, a genetically engineered bacterium with high yield of D-pantothenic acid is constructed by utilizing the keto-alcohol acid reductoisomerase mutant through a CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04%, and compared with an original strain, the valine accumulation amount in obtained fermentation liquor is almost unchanged compared with that of a control, and the yield of D-pantothenic acid is greatly improved. However, the accumulation of branched chain amino acid isoleucine is less, so that the modification of the substrate specificity of the keto-alcohol acid reductoisomerase is achieved.

The invention relates to the technical field of microbial metabolism engineering, in particular to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine as well as a construction method and application of the recombinant escherichia coli. The invention relates to recombinant escherichia coli for efficiently producing O-acetyl-L-homoserine, which is characterized in that a transport protein encoding gene rhtA is knocked out in a chassis strain Escherichia coli W3110, a homoserine O-acetyltransferase encoding gene metXcm from marine cyclobacter is over-expressed, an acetyl coenzyme A synthetase encoding gene acs is over-expressed, pantothenic acid kinase coXpp from pseudomonas putida is over-expressed, and the recombinant escherichia coli can be used for efficiently producing O-acetyl-L-homoserine. And knocking out the malate synthetase coding gene aceB to obtain the recombinant escherichia coli for efficiently producing the O-acetyl-L-homoserine. The strain provided by the invention does not carry plasmids, does not need to additionally add antibiotics and inducers, and is lower in production cost and more stable. And the yield of the O-acetyl-L-homoserine of the plasmid-free strain is increased to 16.85 g / L from 0 g / L. Therefore, the recombinant escherichia coli provided by the invention has important industrial application value.

The invention provides a keto-alcohol acid reductoisomerase mutant and application of the keto-alcohol acid reductoisomerase mutant in construction of genetically engineered bacteria for high yield of D-pantothenic acid. In order to solve the technical problem that in the prior art, the yield of D-pantothenic acid synthesized through microbial fermentation is not high, semi-rational design of enzyme is adopted, and a keto-alcohol acid reductoisomerase mutant with substrate specificity is obtained and applied to construction of genetically engineered bacteria for high yield of D-pantothenic acid. According to the invention, a genetically engineered bacterium with high yield of D-pantothenic acid is constructed by utilizing the keto-alcohol acid reductoisomerase mutant through a CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04%, and compared with an original strain, the valine accumulation amount in obtained fermentation liquor is almost unchanged compared with that of a control, and the yield of D-pantothenic acid is greatly improved. However, the accumulation of branched chain amino acid isoleucine is less, so that the modification of the substrate specificity of the keto-alcohol acid reductoisomerase is achieved.

The invention relates to the technical field of microbial metabolism engineering, in particular to a corynebacterium glutamicum producing strain for efficiently producing D-pantothenic acid as well as a construction method and application of the corynebacterium glutamicum producing strain. The construction method specifically comprises the following steps: (1) by taking wild corynebacterium glutamicum ATCC 13032 as a chassis, weakening ilvA, and constructing DPA1 through mutation expression; (2) knocking out the quinone pyruvate oxidoreductase and inserting a panBCE gene expression cassette into the quinone pyruvate oxidoreductase to construct DPA2; (3) introducing and using a pEC-xk99E overexpression plasmid to screen and express Escherichia coli W3110, Bacillus subtilis 168, and endogenous acetohydroxy acid synthase, acetolactate isomerized reductoisomerase and 2-hydroxy acid dehydratase, and carrying out a step (2) of carrying out a step (3) of screening and expressing the Escherichia coli W3110, Bacillus subtilis 168, and endogenous acetohydroxy acid synthase, acetolactate isomerized reductoisomerase and 2-hydroxy acid dehydratase in the DPA2; and (4) under the control of a strong promoter, carrying out tandem overexpression on the screened ilvBNCD gene by using pEC-xk99E, constructing DPA3, and enhancing a pantothenic acid main synthesis pathway and an upstream pathway pulling force, thereby obtaining the corynebacterium glutamicum for efficiently producing D-pantothenic acid. The strain provided by the invention can produce D-pantothenic acid with high yield and high conversion rate by utilizing a carbon source, and has important industrial application value.

The invention relates to the technical field of biological materials, in particular to a cyclic peptide derivative and a preparation method and application thereof. The cyclopeptide derivative provided by the invention simultaneously comprises a hydrophobic cyclopeptide structure and a hydrophilic pantothenic acid structure which are combined through a covalent ester bond; due to the structure, excellent stability is achieved, and the problems that pantothenic acid is unstable and irritant are effectively solved; after entering the skin, the derivative can be hydrolyzed into pantothenic acid under the action of esterase, and can play a role without oxidative conversion of panthenol. Secondly, the cyclic peptide derivative disclosed by the invention has good biological safety; in an inflammatory cell model, the derivative can more effectively reduce expression of inflammatory factors, and antioxidant activity research shows that the derivative has a strong free radical inhibition effect and can exert excellent anti-inflammatory and antioxidant effects.

The mixture as the micronutrient composition is a combination of alpha-ketoglutaric acid, nicotinamide or nicotinic acid, L-proline, L-glutamine, L-citrulline, sodium pyruvate, pantothenic acid, L-cysteine or N-acetylcysteine, magnesium malate, magnesium citrate, and methionine. The mixture is effective for the treatment of diseases due to oxidative stress. More specifically, the mixtures and cores as micronutrient compositions are effective in reversing saccharification in diabetes mellitus.

The invention provides a beta-alanine producing strain as well as a construction method and application thereof. According to the bacterial strain, an acetaldehyde dehydrogenase gene adhE, a lactic dehydrogenase gene ldhA, an acetokinase gene ackA, a pyruvate dehydrogenase gene poxB, a DNA binding transcription inhibition factor coding gene lacI, an aspartate kinase thrA, a pantothenic acid synthase gene panC, an alanine synthesis transaminase gene cycA and an alanine synthesis transaminase gene yfbQ are knocked out from an E.coli W3110 genome, and the bacterial strain is obtained. A phosphoenolpyruvate carboxylase gene ppc, a pyridine nucleotide transhydrogenase coding gene pntAB, an aspartic acid transaminase gene aspC, an aspartic acid decarboxylase gene panD derived from pseudomonas aeruginosa and a beta-alanine transporter gene NCgl0580 derived from corynebacterium glutamicum are integrated at the same time, and the bacillus subtilis is used for producing beta-alanine and has the advantage of high fermentation yield.

The present invention relates to a composition containing Citrus sinensis (L.) Osbeck fruit and lemon extracts and preferably a trace element, which is preferably a mineral such as Chromium, Calcium, Magnesium, Iron, Copper, Zinc, Iodine, Manganese, Potassium, Sodium, Selenium, Molybdenum, Fluoride, Chloride or Phosphorus or combinations thereof, and their salts and / or a vitamin such as A, D, E, K, B1, B2, C, Niacin, Pantothenic acid, B6, Folic acid, B12 or Biotin or combinations thereof.

The invention relates to the technical field of feed, and discloses a feed additive for laying hen breeding and a preparation method thereof.The feed additive for laying hen breeding is obtained by drying vitamins, nicotinamide, an antioxidant butylated hydroxytoluene, calcium pantothenate, modified chitosan and liquorice, putting the materials into a stirrer, conducting smashing and then conducting mixing. The feed additive obtained by compounding the raw materials can promote the growth of laying hens and promote the organ development of the laying hens; the oxidation resistance and the immunocompetence of the laying hens are improved; amino acid and chitosan groups contained in the modified chitosan also play a better role in helping the growth of the laying hens. The feed additive can shorten the laying day age of laying hens, improve the laying rate, improve the quality of eggs, reduce the yield of inferior eggs, improve the body quality of the laying hens and the like, is a non-toxic, drug-resistant and residue-free laying hen feed additive, and can be widely applied to laying hen feeding feeds.

The present invention relates to a composition for forming a protective barrier in the stomach, aimed at alleviating gastroesophageal reflux disease (GERD) and promoting oral and gastrointestinal health. The composition comprises an alginate complex including Sodium Bicarbonate, Sodium Alginate, Calcium Pantothenate, Ginger (root) Powder, Celery (root) Powder, and Aloe Vera (inner leaf) Powder. The composition forms a gel-like substance upon contact with saliva, creating a physical barrier that minimizes acid reflux. Additionally, the composition is incorporated into a chewing gum, which provides ease of use for the user and promotes overall oral hygiene. The gum includes natural sweeteners for preservation and improved taste, ensuring consumer acceptability.

A bifidobacterium enrichment agent and a use thereof in high-density fermentation. The components of the bifidobacterium enrichment agent include potassium carbonate, arginine, methionine, serine, vitamin C, vitamin B1, vitamin B5, thymine, and calcium pantothenate. The bifidobacterium enrichment agent is added to a basal medium for high-density fermentation, so that the proliferation performance of bifidobacteria can be significantly improved, and the production efficiency can be improved, providing the possibility of preparing a highly active bifidobacterium fermentation liquor, and also providing support for the subsequent preparation and application of bacterial agents.

To provide a composition for hair capable of improving the volume of hair while suppressing the entanglement of hair, and to provide a hair treatment method using the composition for hair.SOLUTION: This composition for the hair is obtained by formulating (A) an amino acid-based cationic surface active agent with (B) one or more kinds selected from pantothenic acids, vitamin C or its salts, vitamin B3 or its salts and vitamin B6 or its salts. The hair treatment method uses the composition for hair.SELECTED DRAWING: None

The invention relates to the technical field of genetic engineering, and discloses an engineering bacterium for high-yield production of D-pantothenic acid based on sugar uptake regulation and a preparation method of the engineering bacterium. The method comprises the following steps: firstly, stabilizing an RNAP promoter open complex formed on an rRNA (Ribonucleic Acid) gene by intensively expressing a necessary global regulation transcription factor CarD of mycobacterium neoaurum; secondly, performing enhanced expression on mycobacterium neoaurum Rpf / Sps family protein RpfB which serves as a resuscitation promoting factor to play a role and catalyze peptidoglycan synthesis to accelerate cell wall growth; meanwhile, the inactivated cell wall lipoprotein Lpp accumulates the outer membrane vesicles; in addition, lipoprotein NlpI responsible for adjusting the activity of endopeptidase MopS and PBP4 is deleted, and the super vesicles are generated; in addition, the mutant membrane lipid asymmetric pathway protein MlaE disturbs the homeostasis of the outer membrane and increases the formation of vesicles; in addition, the membrane anchoring protein TolA is destroyed to improve the membrane permeability; and finally, the escherichia coli gene engineering strain with greatly improved D-pantothenic acid production performance is obtained.

The invention discloses application of an exogenous regulating substance in relieving corn drought stress, and belongs to the technical field of biology. It is verified that external application of serine, pantothenic acid or raffinose can promote root morphology establishment of corn under drought stress, enhance antioxidant enzyme activity of corn roots, reduce MDA accumulation and increase soluble protein content, 0.6 mM of serine, 0.9 mM of pantothenic acid and 1.0 mM of raffinose can effectively relieve stress of the drought environment on corn, and the corn drought resistance can be effectively improved. The water retention capacity of corn is improved, the antioxidant enzyme activity of corn roots is enhanced, the content of osmotic regulation substances is regulated and controlled, damage of drought stress to corn cells is reduced, and meanwhile growth of corn plants and establishment of root morphology are promoted. The invention provides a new strategy for drought-resistant cultivation of corn.

The invention belongs to the technical field of functional food, and particularly relates to a nutritional supplement composition containing various vitamins and mineral substances. The nutritional supplement composition comprises the following components: black pure fat chocolate, vitamin A, vitamin D3, vitamin E, vitamin B1, vitamin B2, vitamin B6, vitamin B12, vitamin C, folic acid, nicotinamide, biotin, calcium pantothenate, heavy magnesium carbonate, ferrous fumarate, zinc gluconate, sodium selenite, copper sulfate, potassium iodide, manganese gluconate and calcium carbonate. The preparation method comprises the following steps: preparing a powder premix; then adding the powder premix into the molten black pure fat chocolate and stirring; the materials are prepared into an inner core containing vitamins and mineral substances through a chocolate forming machine, and then the inner core is coated. The multivitamin mineral sugared pill product provided by the invention is good in taste and stable in effective components, and can effectively relieve fatigue, promote physical and psychological health, improve sleep and improve memory.

The micronutrient composition is a combination of at least 6 ingredients, for example alpha-Ketoglutaric Acid, Niacinamide and / or Niacin, L-Proline, L-Glutamine, L-Citrulline and Sodium Pyruvate, preferably at least 11 ingredients, for example alpha-Ketoglutaric Acid, Niacinamide and / or Niacin, L-Proline, L-Glutamine, L-Citrulline, Sodium Pyruvate, Pantothenic Acid, L-Cysteine and / or N-acetyl Cysteine, Magnesium Malate, Magnesium Citrate and Methionine, more preferably all of the ingredients which are selected from the group consisting of: alpha-Ketoglutaric Acid, Niacinamide and / or Niacin, L-Proline, L-Glutamine, L-Citrulline, Sodium Pyruvate, Pantothenic Acid, L-Cysteine and / or N-acetyl Cysteine, Magnesium Malate and / or Magnesium Citrate, Methionine and / or S- Adenosyl-Methionine (SAMe), Methylsulfonylmethane (MSM), and Wormwood powder and / or extract. The composition is effectively used to treat diseases due to oxidative stress. More specifically, the micronutrient composition is effective in reversing glycation in diabetes and thus in treating diabetes.

The invention discloses construction and application of a recombinant escherichia coli engineering strain with high yield of D-pantothenic acid, and belongs to the technical field of genetic engineering and biological engineering. According to the invention, acetic acid branch pathway genes are knocked out, so that accumulation of precursor pyruvic acid is improved; carrying out free expression and integrated expression on panBC respectively; by weakening a cofactor 5, 10-CH2-THF degradation pathway and strengthening an de novo synthesis pathway of the cofactor 5, 10-CH2-THF, supply of 5, 10-CH2-THF is promoted, and the yield of the constructed engineering strain D-pantothenic acid can reach 4.83 g / L. The Escherichia coli recombinant strain with high yield of D-pantothenic acid, constructed by the invention, lays a foundation for subsequent biosynthesis of D-pantothenic acid and derivatives, and has potential value and significance for development of synthetic biology.

The invention discloses a method for preparing D-calcium pantothenate from fermentation broth, and relates to the technical field of D-calcium pantothenate production.The fermentation broth of D-calcium pantothenate is subjected to flocculation and filter pressing and then enters an ultrafiltration membrane to be filtered, obtained ultrafiltration membrane clear liquid is extracted, subnatant is taken and enters a nanofiltration membrane to be concentrated, obtained concentrated liquid enters a chromatographic column to be eluted, and D-calcium pantothenate is obtained. And collecting a target component containing D-pantothenic acid, then adding a calcium chloride solution for reaction, collecting a reaction solution, cooling and crystallizing, carrying out suction filtration, washing, carrying out suction filtration again, and drying to obtain a finished product of D-calcium pantothenate. According to the method, production links are simplified, the production period is shortened, and the production efficiency is effectively improved; and the purity and yield of the product are improved, the purity is up to 99.5% or above, and the yield is up to 95% or above.

To provide an external preparation that contains 3-10 w / v% of minoxidil, pantothenic acid or a derivative thereof or a salt thereof, and an alcohol, wherein the precipitation of the external preparation is suppressed.SOLUTION: An external composition has a container, and an external composition stored in the container. The external composition contains 3-10 w / v% of minoxidil, pantothenic acid or a derivative thereof or a salt thereof, and an alcohol. Of the container, the portion to be contact the external composition is at least partially composed of polyester.SELECTED DRAWING: None

The present invention discloses the use of pantothenic acid in the preparation of anticaries preparations related to Streptococcus mutans, and anticaries preparations containing pantothenic acid. The invention belongs to the field of biomedicine technology. The anticaries preparations related to Streptococcus mutans include an acid production inhibitor for Streptococcus mutans, a growth inhibitor for Streptococcus mutans, an inhibitor of cariogenic toxins of Streptococcus mutans, and an inhibitor of interspecies competition of Streptococcus mutans. Pantothenic acid can be used to influence the acid production, growth, production of cariogenic toxins, and interspecies competition of Streptococcus mutans, thereby maintaining the balance of the oral microecology.

This invention provides a ketolic acid reductase mutant and its application in constructing genetically engineered bacteria that produce high levels of D-pantothenic acid. To address the technical problem of low D-pantothenic acid yield in existing microbial fermentation, this invention utilizes semi-rational enzyme design to obtain a substrate-specific ketolic acid reductase mutant, which is then applied to construct genetically engineered bacteria that produce high levels of D-pantothenic acid. Furthermore, this invention employs CRISPR-Cas9 gene editing technology to construct genetically engineered bacteria that produce high levels of D-pantothenic acid using the aforementioned ketolic acid reductase mutant, increasing D-pantothenic acid production by approximately 12.04%. Compared to the starting strain, the accumulation of valine in the fermentation broth remained almost unchanged compared to the control, but the accumulation of the branched-chain amino acid isoleucine was lower, achieving substrate-specific modification of the ketolic acid reductase.

The present disclosure relates to multi-enzyme pathways for the in vitro cell-free biosynthesis of the cofactor, coenzyme A ("CoA") from lower-cost substrates, such as D-pantothenate, L-cysteine, and ATP, and the use of these pathways in CoA-dependent cell-free systems for the biobased production of chemicals, such as acetyl-CoA, hexanoyl-CoA, malonyl-CoA, and cannabigerolic acid (CBGA).

The present invention relates to the technical field of bioengineering, and particularly relates to a genetically-engineered bacterium highly-producing D-pantothenic acid independent of the addition of β-alanine, a construction method therefor and the use thereof. By means of gene editing technology, the expression level of a key enzyme in a biological generation pathway of D-pantothenic acid and the expression level of a key enzyme in a biological generation pathway of β-alanine are further enhanced on the basis of existing engineering bacteria, so as to obtain a high-yield bacterial strain which does not need adding β-alanine during a fermentation process. Among all bacterial strains, the maximum shake flask titer reaches 2.97 g / L. Compared with a parent strain, the present invention omits the addition of exogenous β-alanine during the fermentation process, thus greatly reducing the fermentation cost, simplifying the fermentation process, and facilitating large-scale industrial production and application. In addition, the pantothenic acid yield in shake flask fermentation of the genetically-engineered bacterium ZPA18 producing D-pantothenic acid increases to 3.34 g / L, and the yield after replenishment fermentation for 83 h in a 5 L fermentation tank reaches 46.15 g / L.

The invention discloses a production process of anti-hair loss baked grain, which relates to the technical field of baked grain production, comprising 65% chicken, 20% salmon meat, 3% chicken liver, 3% chicken heart, 2% deep-sea fish oil, 2% Antarctic krill oil, brewer's yeast powder, 1% chicken fat, mixed fruit and vegetable powder, cellulose, yucca powder; additive composition: 0.3% manno-oligosaccharide, 0.3% xylo-oligosaccharide, egg yolk lecithin, calcium hydrogen phosphate, serine, sodium hyaluronate for pets, taurine, lysine, methionine, tyrosine, glutamine, chloride Choline, vitamin A, vitamin D3, vitamin E, niacinamide, D-calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin B12, folic acid, biotin, protein zinc, protein iron, protein copper, protein manganese, rosemary extract, tea polyphenols, S1, raw and auxiliary materials collection: S2, meat paste enzymatic hydrolysis, add the minced material into the enzymatic hydrolysis tank, the enzymatic hydrolysis time is one hour, the temperature is 50°-60°, after reaching the specified time, the temperature is raised to inactivate the enzyme, and the material is discharged at the lower temperature. The present invention has the characteristics of high process integration.