The application belongs to the technical field of biological detection, and particularly relates to a probe compound for CoA detection, a fluorescent probe and application, comprising a modified
rhodamine series fluorescent dye, a pantothenate
kinase inhibitor, a HaloTag, a Nanoluc
luciferase and a pantothenate
kinase fusion protein; through
protein labeling, a series of biosensors are obtained,
coenzyme A concentration in living cells can be rapidly detected, and rapid screening of
coenzyme A drugs is regulated; through
rational design and modification of the
chemical probe molecules, and
engineering modification of the sensor proteins, the chemical probes can
label the sensor proteins in living cells, and then the bioluminescent sensors working in living cells are obtained, the requirements of rapid and high-sensitivity detection of CoA can be met, and the drugs capable of regulating the change of
coenzyme A concentration can be high-
throughput screened.