145 results about "Pantothenic acid" patented technology

The invention provides a keto-alcohol acid reductoisomerase mutant and application of the keto-alcohol acid reductoisomerase mutant in construction of genetically engineered bacteria for high yield of D-pantothenic acid. In order to solve the technical problem that in the prior art, the yield of D-pantothenic acid synthesized through microbial fermentation is not high, semi-rational design of enzyme is adopted, and a keto-alcohol acid reductoisomerase mutant with substrate specificity is obtained and applied to construction of genetically engineered bacteria for high yield of D-pantothenic acid. According to the invention, a genetically engineered bacterium with high yield of D-pantothenic acid is constructed by utilizing the keto-alcohol acid reductoisomerase mutant through a CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04%, and compared with an original strain, the valine accumulation amount in obtained fermentation liquor is almost unchanged compared with that of a control, and the yield of D-pantothenic acid is greatly improved. However, the accumulation of branched chain amino acid isoleucine is less, so that the modification of the substrate specificity of the keto-alcohol acid reductoisomerase is achieved.

The invention discloses a fermentation medium for regulating and controlling a clostridium aerovorans target product and a fermentation method of the clostridium aerovorans target product. The medium comprises a basic component and a regulating component, the basic components comprise ammonium chloride, potassium chloride, magnesium sulfate heptahydrate, sodium chloride, monopotassium phosphate, yeast powder, calcium chloride dihydrate, sodium selenite, nickel chloride hexahydrate, biotin, folic acid, pyridoxine hydrochloride, thiamine, riboflavin, nicotinic acid, D-calcium pantothenate, cobalamin, para aminobenzoic acid and lipoic acid; the adjusting component comprises sodium tungstate, ferrous sulfate and sodium molybdate, sodium tungstate and ferrous sulfate have a forward adjusting effect on increasing the yield of acetic acid, ethanol and butanol in the target product, and sodium molybdate has a reverse adjusting effect on the target product. By optimizing the proportion of trace elements in the culture medium, the clostridium metabolism efficiency is improved, synthesis of a target product is regulated and controlled, and the method is particularly suitable for industrial production of acetic acid, ethanol, butanol and the like.

The invention provides a keto-alcohol acid reductoisomerase mutant and application of the keto-alcohol acid reductoisomerase mutant in construction of genetically engineered bacteria for high yield of D-pantothenic acid. In order to solve the technical problem that in the prior art, the yield of D-pantothenic acid synthesized through microbial fermentation is not high, semi-rational design of enzyme is adopted, and a keto-alcohol acid reductoisomerase mutant with substrate specificity is obtained and applied to construction of genetically engineered bacteria for high yield of D-pantothenic acid. According to the invention, a genetically engineered bacterium with high yield of D-pantothenic acid is constructed by utilizing the keto-alcohol acid reductoisomerase mutant through a CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04%, and compared with an original strain, the valine accumulation amount in obtained fermentation liquor is almost unchanged compared with that of a control, and the yield of D-pantothenic acid is greatly improved. However, the accumulation of branched chain amino acid isoleucine is less, so that the modification of the substrate specificity of the keto-alcohol acid reductoisomerase is achieved.

The invention provides a beta-alanine producing strain as well as a construction method and application thereof. According to the bacterial strain, an acetaldehyde dehydrogenase gene adhE, a lactic dehydrogenase gene ldhA, an acetokinase gene ackA, a pyruvate dehydrogenase gene poxB, a DNA binding transcription inhibition factor coding gene lacI, an aspartate kinase thrA, a pantothenic acid synthase gene panC, an alanine synthesis transaminase gene cycA and an alanine synthesis transaminase gene yfbQ are knocked out from an E.coli W3110 genome, and the bacterial strain is obtained. A phosphoenolpyruvate carboxylase gene ppc, a pyridine nucleotide transhydrogenase coding gene pntAB, an aspartic acid transaminase gene aspC, an aspartic acid decarboxylase gene panD derived from pseudomonas aeruginosa and a beta-alanine transporter gene NCgl0580 derived from corynebacterium glutamicum are integrated at the same time, and the bacillus subtilis is used for producing beta-alanine and has the advantage of high fermentation yield.

To provide a composition for hair capable of improving the volume of hair while suppressing the entanglement of hair, and to provide a hair treatment method using the composition for hair.SOLUTION: This composition for the hair is obtained by formulating (A) an amino acid-based cationic surface active agent with (B) one or more kinds selected from pantothenic acids, vitamin C or its salts, vitamin B3 or its salts and vitamin B6 or its salts. The hair treatment method uses the composition for hair.SELECTED DRAWING: None

The invention discloses application of an exogenous regulating substance in relieving corn drought stress, and belongs to the technical field of biology. It is verified that external application of serine, pantothenic acid or raffinose can promote root morphology establishment of corn under drought stress, enhance antioxidant enzyme activity of corn roots, reduce MDA accumulation and increase soluble protein content, 0.6 mM of serine, 0.9 mM of pantothenic acid and 1.0 mM of raffinose can effectively relieve stress of the drought environment on corn, and the corn drought resistance can be effectively improved. The water retention capacity of corn is improved, the antioxidant enzyme activity of corn roots is enhanced, the content of osmotic regulation substances is regulated and controlled, damage of drought stress to corn cells is reduced, and meanwhile growth of corn plants and establishment of root morphology are promoted. The invention provides a new strategy for drought-resistant cultivation of corn.

To provide an external preparation that contains 3-10 w / v% of minoxidil, pantothenic acid or a derivative thereof or a salt thereof, and an alcohol, wherein the precipitation of the external preparation is suppressed.SOLUTION: An external composition has a container, and an external composition stored in the container. The external composition contains 3-10 w / v% of minoxidil, pantothenic acid or a derivative thereof or a salt thereof, and an alcohol. Of the container, the portion to be contact the external composition is at least partially composed of polyester.SELECTED DRAWING: None

The present invention discloses the use of pantothenic acid in the preparation of anticaries preparations related to Streptococcus mutans, and anticaries preparations containing pantothenic acid. The invention belongs to the field of biomedicine technology. The anticaries preparations related to Streptococcus mutans include an acid production inhibitor for Streptococcus mutans, a growth inhibitor for Streptococcus mutans, an inhibitor of cariogenic toxins of Streptococcus mutans, and an inhibitor of interspecies competition of Streptococcus mutans. Pantothenic acid can be used to influence the acid production, growth, production of cariogenic toxins, and interspecies competition of Streptococcus mutans, thereby maintaining the balance of the oral microecology.

This invention provides a ketolic acid reductase mutant and its application in constructing genetically engineered bacteria that produce high levels of D-pantothenic acid. To address the technical problem of low D-pantothenic acid yield in existing microbial fermentation, this invention utilizes semi-rational enzyme design to obtain a substrate-specific ketolic acid reductase mutant, which is then applied to construct genetically engineered bacteria that produce high levels of D-pantothenic acid. Furthermore, this invention employs CRISPR-Cas9 gene editing technology to construct genetically engineered bacteria that produce high levels of D-pantothenic acid using the aforementioned ketolic acid reductase mutant, increasing D-pantothenic acid production by approximately 12.04%. Compared to the starting strain, the accumulation of valine in the fermentation broth remained almost unchanged compared to the control, but the accumulation of the branched-chain amino acid isoleucine was lower, achieving substrate-specific modification of the ketolic acid reductase.

The present disclosure relates to multi-enzyme pathways for the in vitro cell-free biosynthesis of the cofactor, coenzyme A ("CoA") from lower-cost substrates, such as D-pantothenate, L-cysteine, and ATP, and the use of these pathways in CoA-dependent cell-free systems for the biobased production of chemicals, such as acetyl-CoA, hexanoyl-CoA, malonyl-CoA, and cannabigerolic acid (CBGA).

The present invention relates to the technical field of bioengineering, and particularly relates to a genetically-engineered bacterium highly-producing D-pantothenic acid independent of the addition of β-alanine, a construction method therefor and the use thereof. By means of gene editing technology, the expression level of a key enzyme in a biological generation pathway of D-pantothenic acid and the expression level of a key enzyme in a biological generation pathway of β-alanine are further enhanced on the basis of existing engineering bacteria, so as to obtain a high-yield bacterial strain which does not need adding β-alanine during a fermentation process. Among all bacterial strains, the maximum shake flask titer reaches 2.97 g / L. Compared with a parent strain, the present invention omits the addition of exogenous β-alanine during the fermentation process, thus greatly reducing the fermentation cost, simplifying the fermentation process, and facilitating large-scale industrial production and application. In addition, the pantothenic acid yield in shake flask fermentation of the genetically-engineered bacterium ZPA18 producing D-pantothenic acid increases to 3.34 g / L, and the yield after replenishment fermentation for 83 h in a 5 L fermentation tank reaches 46.15 g / L.

The invention discloses application of pantothenic acid in regulating activation of dendritic cells and preparing an immunologic adjuvant. Experiments prove that the pantothenic acid has a remarkable promoting effect on related innate immunity and adaptive immunity of the dendritic cells; various vaccine models find that pantothenic acid can remarkably promote vaccine reactivity and is more efficient than traditional adjuvants, new application of pantothenic acid is provided, the application range of pantothenic acid is expanded, and the pantothenic acid has important economic benefits and social benefits.

The invention relates to a microbial growth activation accelerant based on rare earth elements as well as a preparation method and application thereof, and belongs to the field of resources and environments. The microbial growth activation accelerant based on the rare earth elements is prepared from the following components: 1 to 150g of a rare earth compound, 0.2 to 2g of pantothenic acid, 0.2 to 2g of nicotinic acid, 0.02 to 0.1 g of nicotinamide and 1000g of deionized water, the rare earth compound is one or combination of more of chlorides of lanthanum, cerium, praseodymium, neodymium, promethium, samarium, europium, gadolinium, terbium, dysprosium, holmium, erbium, thulium, ytterbium, lutetium, scandium and yttrium, nitric acid compounds and sulfuric acid compounds. A large number of cheap light rare earth elements are used as raw materials of the microbial accelerator, a new utilization approach is provided for the light rare earth elements, the light rare earth elements are converted into high-added-value products, pollution of the light rare earth elements to the environment is reduced, and meanwhile new elements are introduced into the field of microbial accelerators. More importantly, the preparation method of the microbial growth activation accelerant based on the rare earth elements provided by the invention is simple in production process and convenient for large-scale production.

The invention discloses a genetically engineered bacterium for efficiently producing D-pantothenic acid as well as a construction method and application of the genetically engineered bacterium, the construction method comprises the following steps: (a) taking corynebacterium glutamicum DPA2 as a chassis bacterium, enhancing the expression of at least one pantothenic acid main pathway gene in a chassis bacterium genome, and introducing plasmid pEC-XK99E-panBCilvC to obtain an engineered strain for modifying the pantothenic acid main pathway gene; (b) knocking out at least one heteroacid pathway gene in the engineering strain obtained in the step (a), carrying out in-situ integration on a panBCC gene at an alaT gene locus, and introducing a plasmid pEC-XK99E-panBCilvC to obtain a heteroacid pathway modified engineering strain; and (c) knocking out the pyruvic acid shunt gene pyc in the heteroacid pathway modified strain obtained in the step (b), enhancing the expression of an odx gene, and introducing a plasmid pEC-XK99E-panBCilvC to obtain the engineering strain for regulating and controlling the pyruvic acid shunt. The genetically engineered bacterium has the beneficial effects that the genetically engineered bacterium for efficiently producing D-pantothenic acid is obtained, and the yield and the conversion rate are higher; the level of the modified strain in the aspect of fermentation production of D-pantothenic acid is obviously improved.

The invention relates to an adipose-derived stem cell culture medium containing wormwood extract and a preparation method of the adipose-derived stem cell culture medium, and belongs to the technical field of biology. The adipose-derived stem cell culture medium contains a DMEM (Dulbecco Modified Eagle Medium) basal culture medium, and also contains the following components: 5 to 7 [mu] g / mL of wormwood extract, 1 to 1.5 [mu] g / mL of phosphatidylserine, 0.02 to 0.03 [mu] g / mL of D-calcium pantothenate, 1.2 to 1.5 [mu] g / mL of sialic acid, 0.06 to 0.08 [mu] g / mL of eucommia male flower peptide, 0.3 to 0.35 [mu] g / mL of transferrin and 0.3 to 0.4 mg / mL of bovine serum albumin. The prepared adipose-derived stem cell culture medium has a good promoting effect on proliferation of adipose-derived stem cells from rabbits and mice, the added wormwood polysaccharide extract has a good bacteriostatic effect, and the safety and stability of the culture medium can be effectively maintained.

The invention provides a skin repair liquid and a preparation method thereof, the skin repair liquid comprises a phase A, a phase B, a phase C, a phase D and a phase E. The skin repair liquid comprises the following components in parts by weight: 53-63 parts of the phase A, 36-44 parts of the phase B, 0.5-1.5 parts of the phase C, 0.3-0.7 part of the phase D and 0.4-0.6 part of the phase E. The phase A is panthenol, the phase B is a lactobacillus / soybean milk fermentation product filtrate soothing agent, and the phase C is a transdermal polypeptide stock solution. The panthenol can permeate the stratum corneum to be converted into pantothenic acid, participates in cell energy metabolism and promotes epidermal regeneration and barrier repair; the lactobacillus / soybean milk fermentation product filtrate relieving agent is used for regulating the diversity of skin microorganisms and relieving inflammation; the penetration efficiency of active ingredients of the transdermal polypeptide stock solution is enhanced through transdermal oligopeptide, so that macromolecular protein substances break through a skin barrier; the phase D is p-hydroxyacetophenone, and the phase E is 1, 2-hexanediol; the comprehensive effects of moisturizing, relieving, repairing and transdermal absorption are achieved through the synergistic effect of multiple components, and the problem that the repairing effect of a traditional skin repairing liquid is limited is solved.

The present invention provides a novel process for producing panthenol from pantothenic acid or salts and / or esters thereof. The process makes a “hybrid process” possible to produce panthenol, which is generally more eco-friendly and sustainable and reduces carbon print compared to a pure chemical process.

The present invention provides a recombinant microorganism for simultaneously producing sesquiterpenes and primary metabolites, wherein the primary metabolites include at least one of pantothenic acid, squalene, fatty acids and vitamin B2; the strain contains the mevalonate pathway, the β-farnesene synthesis gene and genes related to the synthesis of primary metabolites; the genes related to the mevalonate pathway include ERG10, ERG13, tHMG1, ERG12, ERG8, MVD1, IDI1 and ERG20 genes, and the copy numbers are ERG10:ERG13:tHMG1:ERG12:ERG8:MVD1:IDI1:ERG20 = 2:2:X:2:2:2:2:2, where X is an integer greater than or equal to 1; the β-farnesene synthesis gene includes bFS, and the sequence is as shown in SEQ ID NO.1. The present invention realizes high yield of primary metabolites while producing sesquiterpene compounds.

The invention discloses a method for extracting and preparing D-calcium pantothenate from fermentation broth, and relates to the technical field of D-calcium pantothenate production.The method comprises the steps that a chitosan flocculant is added into the fermentation broth, standing and flocculation are conducted, then calcium oxide is added, kieselguhr is added, plate frame clear liquid is filtered through an ultrafiltration membrane, ultrafiltration membrane clear liquid is concentrated through a nanofiltration membrane, purified water is added for dialysis, and the D-calcium pantothenate is obtained; desalting the obtained dialysate in cation exchange resin, adding calcium hydroxide into the desalted solution to adjust the pH value, concentrating the obtained neutralized solution, adding ethanol into the concentrated solution, cooling and crystallizing, filtering by a centrifugal machine after cooling to obtain a crude product, and drying to obtain a finished product. And anion exchange resin with higher price is not used, so that the method is environment-friendly, and meanwhile, the production cost is saved.

The invention discloses a pantothenic acid synthase mutant with heat resistance and long half-life period, and relates to the field of biochemistry, the nucleotide sequence of the pantothenic acid synthase mutant N54R / K115R / Q148R is SEQ ID NO.1, and the amino acid sequence of the pantothenic acid synthase mutant N54R / K115R / Q148R is SEQ ID NO.2. The invention further discloses a preparation method of the pantothenic acid synthase mutant. The relative enzyme activity is improved by 43% compared with the original plasmid, and the catalytic efficiency is higher. And at 35 DEG C, 40 DEG C and 45 DEG C, the enzyme activity residual rate and the half-life period of the pantothenic acid synthetase mutant are obviously higher than those of an original plasmid, and the yield of D-pantothenic acid and the substrate conversion rate are obviously increased.

The invention belongs to the technical field of aquaculture, and particularly relates to a decapterus maruadsi breeding feed and application thereof in improving the quality of decapterus maruadsi fertilized eggs. The invention provides a decapterus maruadsi breeding feed. The feed comprises a first feed and a second feed which are mutually independent, the first feed comprises a basal feed, fish cream and a nutrition enhancer; the second feed comprises the first feed and oyster meat; the nutrition enhancer contains calcium pantothenate, vitamin B1, vitamin B2, vitamin B6, vitamin C and vitamin F. When the culture feed for the decapterus maruadsi is used for performing nutrient enrichment on parent fishes of the decapterus maruadsi, synchronous development of sexual glands of male and female parent fishes of the decapterus maruadsi can be promoted, the quality of fertilized eggs of the decapterus maruadsi is improved, the good egg rate of the fertilized eggs of the decapterus maruadsi reaches 88.50% and is 39.15% higher than that of a control group, and the fertilization rate of the fertilized eggs reaches 79.58% and is 19.46% higher than that of the control group.

The present application relates to the technical field of genetic engineering, and discloses a genetically engineered bacterium for high-yield D-pantothenic acid, a construction method and application.The genetically engineered bacterium for high-yield D-pantothenic acid is obtained by knocking out an ilvA gene in Corynebacterium glutamicum ATCC 13032 to reduce the consumption of pyruvic acid for isoleucine generation, overexpressing a 3-methyl-2-oxobutyric acid hydroxymethyltransferase gene panB and a pantothenate synthetase gene panC in a pantothenic acid synthesis pathway, and heterologously overexpressing a pantothenate reductase panE from Escherichia coli to further improve the yield of D-pantothenic acid in the Corynebacterium glutamicum ATCC 13032.

The invention discloses a genetically engineered bacterium for producing D-pantothenic acid as well as a construction method and application of the genetically engineered bacterium. The genetically engineered bacterium takes DPA11A * 01 as an original strain, the copy number of ilvD, serA and glyA genes regulated by a promoter pTrc is increased on a genome of the original strain, an ldhA gene, an mgsA gene, a poxB gene and an adhE gene are knocked out, and an alsS gene from bacillus subtilis is over-expressed. After the strain for producing D-pantothenic acid is fermented in a 250 mL shake flask for 48 hours, the DPA yield reaches 7.84 g / L and is increased by 26% compared with an original strain. When the strain is fermented for 100 hours by using a 5L-fermentation tank, the highest yield is 72.4 g / L, the total sugar consumption is 500g when the fermentation is finished, the final fermentation volume is 3L, the sugar-acid conversion rate is 43.4% and is improved by 17.5% compared with that of an original strain DPA11A * 01, and the sugar-acid conversion rate is obviously improved.

The application discloses a production process of a feed-grade high-protein D-calcium pantothenate particle, and the process comprises the following steps: (1) after inactivation of D-calcium pantothenate mash, ceramic filtrate and ceramic membrane residue liquid are obtained through membrane filtration; (2) after heating of the ceramic filtrate, wood active carbon is added for decolorization; decolorized liquid is obtained through suction filtration; (3) the decolorized liquid is mixed with part of the ceramic membrane residue liquid to prepare a mixed liquid with a dry basis purity of 52-58%; (4) after concentration of the mixed liquid to a dry solid content of 52-58%, auxiliary materials are added to prepare a dry basis purity of 42-48%, and a 42-48% D-calcium pantothenate particle product is obtained through granulation bed drying. The production process effectively reduces the production cost and environmental protection pressure, and meanwhile, the bacterial protein and auxiliary materials are used to prepare an animal-friendly feed D-calcium pantothenate product which is better in flowability and richer in nutrition.

The present invention relates to a nutritional vitamin complex for pets intended to improve the fur and skin quality of pets such as dogs and cats. The composition of the complex contains omega-3, curcumin, piperine, biotin (vitamin H or vitamin B7), vitamin B2 (riboflavin), vitamin B3 (nicotinic acid or niacin), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine), vitamin B12 (cyanocobalamin), folic acid (vitamin B9), vitamin E (alpha-tocopherol), vitamin C, zinc (zinc glycine chelate), coenzyme Q10, hydrolysed type II collagen, astaxanthin, DL-methionine, selenium, L-glycine, L-proline, L-phenylalanine, L-leucine, L-isoleucine, L-histidine, L-arginine, L-lysine, L-tyrosine, resveratrol, and a flavouring agent.

To provide a skin external composition which is an O / W emulsion having selective antibacterial and bactericidal properties, suppressed oil-water separation, suppressed skin irritation and an excellent moisturizing sensation.SOLUTION: There is provided a skin external composition which is an O / W emulsion and comprises (A) one or more selected from pantothenic acid or the like, (B) one or more surfactants which are selected from a polyethylene glycol fatty acid ester, a polyoxyethylene sorbitol fatty acid ester, a sorbitan fatty acid ester, a polyglycerol fatty acid ester, a polyoxyethylene glycerol fatty acid ester and a polyoxyethylene alkyl ether and have an HLB of 8 to 16 and (C) one or more selected from N-myristoyl-L-glutamate, N-stearoyl-L-glutamate, myristoyl methyl taurate, N-stearoyl-N-methyl taurate and stearoyl lactate.SELECTED DRAWING: None

The utility model relates to the technical field of D-calcium pantothenate production, in particular to a production system for recycling D-calcium pantothenate mother liquor, which comprises a first concentration kettle communicated with a mother liquor conveying pipeline, a concentrated liquor outlet of the first concentration kettle is communicated with a decolorizing kettle, and a decolorizing liquor outlet of the decolorizing kettle is communicated with a cationic resin column. An effluent outlet of the cationic resin column is communicated with an anion resin column, a feed port of the anion resin column is communicated with an eluent conveying pipeline, an eluent outlet of the anion resin column is communicated with a second concentration kettle, a concentrated solution outlet of the second concentration kettle is communicated with a crystallization kettle, and a solvent inlet of the crystallization kettle is communicated with an ethanol conveying pipeline; a crystal outlet of the crystallization kettle is communicated with the drying device; a discharge hole of the drying device is communicated with the product storage tank. According to the production system with the structure, effective recovery treatment of mother liquor calcium pantothenate is realized, resource waste is avoided, the yield, the yield and the purity of D-calcium pantothenate are greatly improved, and economic benefits are increased for enterprises.

The application provides a ketol-acid reductoisomerase mutant and application thereof in construction of a genetically engineered bacterium with high D-pantothenic acid yield. In order to solve the technical problem of low yield of D-pantothenic acid synthesized by microbial fermentation in the prior art, the application uses semi-rational design of enzymes to obtain a ketol-acid reductoisomerase mutant with substrate specificity, and applies the mutant to construction of a genetically engineered bacterium with high D-pantothenic acid yield. The application also uses the ketol-acid reductoisomerase mutant to construct a genetically engineered bacterium with high D-pantothenic acid yield by CRISPR-Cas9 gene editing technology, so that the yield of D-pantothenic acid is increased by about 12.04 %, and compared with a starting strain, the accumulation amount of valine in the obtained fermentation liquor is almost unchanged compared with a control, but the accumulation of branched-chain amino acid isoleucine is less, achieving modification of the substrate specificity of the ketol-acid reductoisomerase.

The present invention relates to a nutritional vitamin complex for pets intended to improve the fur and skin quality of pets such as dogs and cats. The composition of the complex contains omega-3, curcumin, piperine, biotin (vitamin H or vitamin B7), vitamin B2 (riboflavin), vitamin B3 (nicotinic acid or niacin), vitamin B5 (pantothenic acid), vitamin B6 (pyridoxine), vitamin B12 (cyanocobalamin), folic acid (vitamin B9), vitamin E (alpha-tocopherol), vitamin C, zinc (zinc glycine chelate), coenzyme Q10, hydrolysed type II collagen, astaxanthin, DL-methionine, selenium, L-glycine, L-proline, L-phenylalanine, L-leucine, L-isoleucine, L-histidine, L-arginine, L-lysine, L-tyrosine, resveratrol, and a flavouring agent.