36 results about "Chicken fat" patented technology

The utility model relates to the technical field of chicken oil storage, and discloses an anti-precipitation chicken oil storage tank, which comprises a bottom plate, an oil storage tank body and an oil delivery pump which are mounted at the top of the bottom plate, and an oil pumping pipe communicated with the lower part of the surface of the oil storage tank body and communicated with an oil inlet of the oil delivery pump, an oil outlet of the oil delivery pump is communicated with an oil delivery pipe; chicken oil located on the lower portion in the oil storage tank body can be pumped out and input into the oil storage tank body from the upper portion in the oil storage tank body again so as to reduce the precipitation phenomenon, meanwhile, the oil storage tank can stir precipitates at the bottom of the inner wall of the oil storage tank body and the chicken oil, the chicken oil is continuously conveyed upwards, and the oil storage effect is improved. The problems that at present, part of chicken oil is stored in an oil storage tank for a long time, precipitation and layering are prone to occurring, and the quality of pet food made through follow-up processing is prone to being affected are solved.

The invention discloses a chicken myoblast and chicken fat precursor cell co-culture synchronous differentiation method and application thereof, and belongs to the technical field of animal cell co-culture and differentiation. The method comprises the following steps: respectively carrying out in-vitro multiplication culture on chicken myoblasts and chicken fat precursor cells, carrying out mixed co-culture, inducing for at most two days by adopting horse serum with the volume percent of 0.5-2%, carrying out pre-differentiation, and continuously carrying out induced differentiation culture for at most one day by using an induced adipogenesis culture medium. According to the synchronous differentiation method for co-culture of the chicken myoblasts and the chicken fat precursor cells, a cell co-culture system is constructed, time sequence induced differentiation is achieved, and a final product with fat droplets distributed in the gaps of muscle bundles (the average distance is 20-50 microns) and capable of recarving natural chicken intermuscular fat textures is obtained. According to the method, the differentiation period is shortened to be within 3 days from 21 days (the efficiency is improved by 600%); the induction cost is reduced by 42.7%; the final product breaks through the sensory acceptance barrier through bionic structure recarving.

The utility model discloses a refining device for chicken oil production. The refining device comprises a refining shell and a rapid stirring mechanism, a metal heating pipe is arranged in an inner cavity of the wall body of the refining shell, a conical hopper, a filtering plate and a discharging plate are sequentially arranged in the refining shell from top to bottom, and an air outlet pipe and a feeding pipe are arranged on the top wall of the refining shell in a penetrating mode; the rapid stirring mechanism is arranged in the refining shell; wherein the refining device further comprises a single-chip microcomputer, the single-chip microcomputer is arranged outside the refining shell, the input end of the single-chip microcomputer is electrically connected with an external power source, the output end of the single-chip microcomputer is electrically connected with the input end of the metal heating pipe, and the rapid stirring mechanism comprises a driving shaft, a rotating rod, an auxiliary stirring rod, a main stirring rod and a hollow shaft. The stirring part rotates forwards and backwards through the transmission element, so that the chicken fat in the stirring part is fully stirred, the heating area of the chicken fat is increased, and the chicken fat refining efficiency of the device is improved.

The utility model relates to the technical field of chicken fat feed production, in particular to a presser convenient for residue cleaning for chicken fat feed production, which comprises a rack, a pressing roller is rotatably connected onto the rack, and a pressing filter screen cylinder with a residue outlet groove penetrating through the pressing roller is fixedly mounted in the pressing roller. One side of the squeezing roller is rotationally connected with a feeding pipe communicated with the squeezing filter screen cylinder, the feeding pipe is connected with the rack, a feeding cover is fixedly installed at the top of the end, away from the squeezing roller, of the feeding pipe, a scattering and conveying screw rod is rotationally connected into the feeding pipe, and a rotating frame is rotationally connected into the squeezing filter screen cylinder; according to the presser for chicken fat feed production, continuous feeding and pressing are facilitated, residues are synchronously scraped in the pressing process, pressing is convenient, and meanwhile the residues are convenient to clean.

The invention relates to a large-particle dog and cat oral health-care functional food wrapped with instant freeze-dried particles and a preparation method thereof, and belongs to the technical field of pet food. According to the scheme, an external spraying system comprises grain base material particles, 0.1 to 0.15 part of lecithin, 0.002 to 0.003 part of monostearin, 0.001 to 0.003 part of vitamin E and 0.8 to 1.2 parts of chicken oil, and an instant freeze-dried particle layer comprises 0.1 to 0.2 part of fucoidan, 0.02 to 0.05 part of lysozyme, 0.04 to 0.08 part of tea polyphenol, 0.02 to 0.04 part of zinc gluconate, 0.05 to 0.1 part of Arabic gum, 0.03 to 0.1 part of glutamine, 0.01 to 0.02 part of vitamin B2, 0.01 to 0.04 part of vitamin B12 and 0.02 to 0.07 part of glycine. The toothpaste can effectively inhibit the formation of dental plaque and dental calculus and remove halitosis.

FIELD: veterinary medicine and dietetics.SUBSTANCE: development of complete feed compositions that can provide a therapeutic effect in cases of exocrine pancreatic insufficiency. The composition of a complete dietary feed for cats with exocrine pancreatic insufficiency has been developed in the following ratio, wt.%: grain part: mixture of rice, wheat, hulled barley and corn – 37.0; protein part of plant origin: isolated soy protein, beet pulp, wheat bran, flax seeds – 22.0; protein part of animal origin: feather meal, chicken liver, egg powder – 25.0; mineral supplement: feed chalk, monocalcium phosphate, table salt, plantain seeds Plantago psyllium, mannanoligasaccharides with beta-glucans and lutein – 2.0; vitamin premix: A, D3, E, K, B1, B2, B3, B5, B6, B12, Bc, H, taurine, inulin, beech lignocellulose, complex of natural fulvic and humic acids – 2.5; flavor enhancer in the form of enzymatic hydrolysate of pork and chicken by-products – 3–3.5; brewer's yeast – 2.5–3.0; a mixture of fish and chicken fat – 5.0–6.0.EFFECT: obtaining food for cats with exocrine pancreatic insufficiency, which is easily reproducible, clinically effective, and convenient in dosing and use.1 cl, 3 tbl, 1 ex

The utility model relates to the technical field of chicken oil processing, and discloses a condensing device for chicken oil processing, which comprises a mounting plate, a mounting piece mounted on the top end face of the mounting plate, a cooling mechanism fixedly mounted on the top end face of the mounting piece, and an extraction mechanism fixedly mounted on the top end face of the mounting plate. When chicken oil needs to be cooled, a first valve is opened, extracted chicken oil is conveyed into a condensation pipe through a connecting pipe, at the moment, a pump machine is opened, cooling water in a water tank is conveyed into a cooling water circulation assembly through a conveying pipe by the pump machine for cooling, and after cooling, the cooling water is pumped into a cooling coil through the pump machine; the condensation pipe is cooled through the cooling coil, then cooling operation is conducted through the condensation pipe, the temperature in the condensation pipe can be further observed through the thermometer, temperature control is facilitated, after condensation is completed, the second valve is opened, the condensed chicken oil can be discharged through the oil outlet pipe, and the cooling requirement during chicken oil production can be met; and the practicability of the device is improved.

The present application relates to a kind of chicken oil refining process, it is related to animal oil technology field, wherein the refining process includes pretreatment, low-temperature degumming, deacidification and decolorization, low-temperature deodorization, ceramic membrane filtration.The refining process of the present application is through the steps such as biological enzyme directional degumming, charged membrane separation deacidification and decolorization, nitrogen microbubble low-temperature deodorization, realizes the efficient refining of chicken oil at low temperature, so that the nutrient substance of chicken oil is not oxidized.

This invention relates to the field of feed additive technology, specifically to a feed additive that promotes digestion and absorption and its preparation process. The preparation process of a feed additive that promotes digestion and absorption includes: preparing antioxidant-modified chicken fat; preparing an extract; preparing a composite oil phase; and preparing the feed additive. In this invention, the extract of Shenqu (a type of fermented wheat) can directly compensate for insufficient endogenous enzyme secretion in laying hens, rapidly decomposing starch in feed and chicken fat in plant proteins, laying the foundation for subsequent absorption. The extract of Zhishi (a type of immature bitter orange) accelerates gastrointestinal motility and digesta emptying, while also promoting bile and pancreatic juice secretion, further improving the digestibility and utilization rate of fat-soluble components in laying hens. The extract of pine pollen can repair the intestinal mucosa, increase intestinal villus height, increase the nutrient absorption area, and regulate the intestinal microecology, reducing nutrient loss and intestinal stress. The combined use of these three ingredients synergistically promotes the digestive and absorptive capacity of laying hens.

The invention discloses a method for preparing chicken powder through lipase hydrolysis and self-emulsification, which comprises the following steps: putting chicken paste and chicken skeleton into a horizontal high-temperature cooking pot, and carrying out cooking heat treatment to obtain cooked meat solid and a soup oil-water mixture; then crushing, mixing and grinding into thick liquid, and adding into a high-shear emulsifying tank; adding lipase and glycerol, and carrying out enzymolysis at 50-60 DEG C for 1-3 hours at the rotating speed of 2000-3000r / min to generate a monoglyceride mixture emulsifier; carrying out self-emulsification on a product after enzymolysis, wherein the homogenizing pressure is 15-25MPa; and carrying out spray drying on the self-emulsified feed liquid to obtain a chicken powder product. According to the method, firstly, chicken skeletons and chicken paste are cured, then glycerol is added, high-shear emulsification is used for assisting lipase enzymolysis, the characteristic flavor of chicken fat is released, finally, self-emulsification is carried out, the particle size of oil drops is reduced from 30-60 microns to about 10 microns, then spray drying is carried out, the flowability of materials is improved, and the recovery rate reaches 95% or above.

The invention discloses a chicken body fat rate phenotype determination system and a chicken selection method. The chicken body fat rate phenotype determination system comprises an eight-electrode system, a multi-channel signal receiving and transmitting module, a processor module and a power supply module, the eight-electrode system is used for sending excitation current and receiving potential difference signals, and a local closed-loop measurement loop can be formed on the same limb; the multi-channel signal receiving and transmitting module is used for controlling excitation and measurement among the electrode groups and realizing biological impedance data acquisition in multiple directions; the processor module is used for constructing data models of the upper limbs, the trunk and the lower limbs of chicken body fat distribution according to the multi-channel impedance data; the power supply module is connected with the processor module and the eight-electrode system and provides power for the system. The method adopts the bioelectrical impedance value to measure and detect the body fat rate of the live chicken, and is high in detection precision, convenient to operate, high in adaptability and convenient to popularize and apply in the field of poultry breeding.

This invention discloses an enhancer that regulates chicken PGC-1α gene expression and its applications, belonging to the field of bioengineering. The nucleotide sequence of the enhancer is shown in SEQ ID NO: 1. This invention further discloses biomaterials containing this enhancer and a method for preparing cell lines with the enhancer knocked out. A dual-luciferase reporter gene system confirmed its significant activity in DF1 and ICP2 cells. Knocking out this enhancer downregulates PGC-1α gene expression in chicken preadipocytes, significantly promoting cell differentiation and lipid droplet formation. This enhancer can be used to prepare biological agents that regulate PGC-1α gene expression levels and chicken preadipocyte differentiation. It can also be applied to the genetic improvement of chicken fat traits and to the preparation of models for studying related gene function and cell differentiation mechanisms. This invention provides new targets and technical support for improving chicken fat traits.

The mushroom soup flavored hotpot condiment comprises the following components in parts by weight: 350 parts of beef tallow; 50 parts of dried mushroom; 40 parts of chicken oil; 30 parts of lard oil; 15 parts of onions; 9 parts of garlic; 15 parts of ginger; 42 parts of edible salt; 15 parts of monosodium glutamate; 2 parts of red dates; 1.5 parts of fructus lycii; 5 parts of dried shrimps; 12 parts of preserved szechuan pickles; 22 parts of chicken essence seasoning; and 10 parts of spices. The mushroom soup flavored hotpot condiment provided by the invention is wide in raw material source and stable in quality, the production cost can be effectively reduced, and the characteristics of delicious taste and rich nutrition are reserved.

The utility model relates to the technical field of residue scraping, in particular to a pool wall residue scraping device for a chicken oil sedimentation pool, which comprises a pool wall residue scraping machine, tightening assemblies are arranged on two sides of the pool wall residue scraping machine, and a height adjusting assembly is mounted below the pool wall residue scraping machine. And the tightening assembly comprises a lead screw, a guide block is fixedly installed below the lead screw, the outer wall of the lead screw is connected with a nut pair, and a rotating rudder is fixedly arranged on the outer wall of the nut pair. According to the pool wall slag scraping device, due to the arrangement of the tightening assembly, the toothed chain of the pool wall slag scraping machine can be tightened, operation is easy and fast, it can be guaranteed that the scraping plate of the pool wall slag scraping machine effectively scrapes oil residues, efficiency is improved, due to the arrangement of the height adjusting assembly, the height adjusting plate is located at the same height as chicken oil, and the oil residue scraping efficiency is improved. Bolts penetrate through holes of the fixing plate and the limiting plate to be tightened, the position of the height adjusting plate is fixed, the position of the height adjusting plate is conveniently adjusted, and then the chicken oil residues of different heights are cleaned.

The invention discloses application of a transcription factor E2F5 for regulating and controlling chicken fat formation, and relates to the field of animal molecular heredity and developmental biology. Differentiation of chicken preadipocytes is inhibited through overexpression of the transcription factor E2F5, differentiation of the chicken preadipocytes is promoted through interference of the E2F5, and the transcription factor KLF7 plays a regulating role by regulating the enhancer activity of the transcription factor E2F5.

The invention discloses an enhancer for regulating and controlling chicken PGC-1alpha gene expression and application of the enhancer, and belongs to the field of bioengineering. The nucleotide sequence of the enhancer is shown as SEQ ID NO: 1. The invention further discloses a biological material containing the enhancer and a preparation method of a cell strain with the enhancer knocked out. A dual-luciferase reporter gene system proves that the enhancer has remarkable activity in DF1 and ICP2 cells, the expression of the PGC-1alpha gene in chicken preadipocytes can be reduced by knocking out the enhancer, and cell differentiation and lipid droplet generation are remarkably promoted. The enhancer can be used for preparing a biological preparation for regulating and controlling the expression level of the PGC-1alpha gene and regulating and controlling chicken preadipocyte differentiation, can also be applied to genetic improvement of chicken fat traits and can be used for preparing a model for researching related gene functions and cell differentiation mechanisms. The invention provides a new target and technical support for chicken fat character improvement.

The invention provides a method for high-temperature pressurized extraction of scallion-flavored chicken oil, which comprises the following steps: mixing chicken fat and auxiliary materials, extracting under 0.15-0.18 MPa and 115-120 DEG C, and carrying out crude separation to obtain an oil-water mixture; the auxiliary materials comprise green Chinese onion, onion, ginger and spices; and centrifugally drying the oil-water mixture to obtain the scallion-flavored chicken oil. According to the present invention, the high temperature and high pressure conditions are adopted, the chicken fat raw material and the auxiliary materials (including green Chinese onion, onion, ginger and spice) are mixed, extraction is performed together, chicken oil extraction and seasoning are completed in one step, the nutritional value and the characteristic flavor of the chicken oil are maintained to the maximum extent, the physical and chemical indexes such as the acid value and the peroxide value of the finished product are effectively reduced, and the safety of the finished product is improved.

The invention discloses a chicken fatty liver syndrome grading method based on oil red O staining, belongs to the technical field of poultry genetic breeding, and is used for efficiently and quantitatively grading the severity of chicken fatty liver syndrome. The method comprises the following steps: firstly, performing paraffin section Hamp on liver tissue; and E, identifying whether the high-fat hens and the low-fat hens suffer from the fatty liver syndromes or not by a staining method, and grading the severity of the diseases. Then, Hamp is used; and respectively analyzing the ranges of oil red O staining quantitative liver fatty degeneration degrees corresponding to different fatty liver syndrome grades of the high-fat hens and the low-fat hens by taking the E staining chicken fatty liver syndrome grading result as a reference, and establishing an oil red O staining chicken fatty liver syndrome grading standard. According to the method, the severity of the chicken fatty liver syndrome can be rapidly and quantitatively graded, and reference is provided for related research.

The invention discloses a method for improving eating and safety quality of salt roasted chicken based on low-temperature plasma and EGCG (epigallocatechin gallate) treatment. The preparation method comprises the following steps: by taking fresh chicken as a raw material, cleaning, removing hair, removing fat and fascia, pickling with salt and cooking wine, soaking in prepared marinating soup for marinating, treating with dielectric barrier discharge type low-temperature plasma equipment and soaking with an EGCG (epigallocatechin gallate) solution, and carrying out vacuum packaging and cold storage. The electric field discharge frequency of the dielectric barrier discharge type low-temperature plasma equipment is controlled to be 25-35 kHz, and the discharge voltage is controlled to be 40-60 kV; an EGCG aqueous solution with the mass concentration of 0.05-0.15% is controlled to be adopted in the EGCG soaking treatment, and the soaking time is 10-15 min. According to the invention, the shelf life of the salt-roasted chicken can be prolonged by 14 days, fat oxidation and volatile basic nitrogen accumulation of the salt-roasted chicken are delayed, the color and texture of the salt-roasted chicken are maintained, and the comprehensive commodity value of the salt-roasted chicken is improved.

The invention relates to an animal feed and its manufacturing process. The raw material used contains the following components: meat and products of animal origin from 50 to 80% by weight; fish and fish products from 4 to 36% by weight; molluscs and crustaceans from 0 to 14% by weight; poultry eggs from 3 to 7% by weight; starch from 3 to 7% by weight; vegetables and / or fruit from 0 to 10% by weight; vegetable glycerin from 0 to 8% by weight; mineral ingredients from 0 to 2% by weight; oils from 0.1 to 2% by weight; powdered cellulose from 0.1 to 2% by weight; functional ingredients from 0.01 to 0.8% by weight; mineral-vitamin mixture from 0.1 to 0.2% by weight; herbs from 0.01 to 0.05% by weight. Fat for coating (advantageous addition of a natural antioxidant, preferably beef or chicken fat) of 1 to 6% by weight.

The invention relates to the technical field of poultry breeding, and particularly provides a novel application of miR-363-5p. A hepatocyte model is established through overexpression and knock-down of the miR-363-5p so as to detect key indexes related to lipid synthesis, oxidative stress and inflammatory factors, and research results show that inflammatory response in chicken hepatocytes can be inhibited, oxidative stress can be reduced and lipid degradation can be prevented by reducing expression of the miR-363-5p. In addition, the invention also finds that the development of chicken liver cells is controlled by targeting the miR-363-5p to the CPEB2, ERK signal transduction can be activated by knocking down the miR-363-5p or overexpressing the CPEB2, so that the miR-363-5p-CPEB2-MAPK / ERK axis is determined to be a key regulatory factor in the pathogenesis of the chicken fatty liver. Therefore, the miR-363-5p and / or CPEB2 gene can be used as a detection target to develop a reagent or a kit for diagnosing the chicken fatty liver syndrome.

The application discloses a chicken oil storage tank, and relates to the technical field of oil storage tanks, which comprises a tank body, and a supporting base is fixedly installed around the bottom of the tank body; the stirring paddle is continuously rotated to stir the chicken oil stored in the tank body, the rotation of the stirring paddle can fully mix the chicken oil in different parts of the tank, the rotation of the stirring paddle can fully mix the chicken oil in different parts of the tank, continuous stirring can fully contact the chicken oil with air, accelerate the consumption and update of oxygen in the chicken oil, reduce the oxidation reaction caused by the excessively high local oxygen concentration, in addition, the rotation of the stirring paddle can keep the chicken oil in a constant motion state, reduce the residence time and adhesion opportunity of the chicken oil on the tank wall, thereby preventing the chicken oil from forming a thick deposition layer on the tank wall and reducing the risk of tank wall corrosion caused by long-term adhesion.

This utility model discloses a high-efficiency pressing device for producing chicken oil as a feed additive, including a base on which a pressing device is mounted. A coupling device is also provided on the base. The coupling device includes a bushing, a shaft, a rotating sleeve, a slot, an inclined groove, an inclined plate, a linkage groove, a linkage sleeve, a linkage block, and a movable block. The slot is located on the outside of the shaft, the inclined groove is located on the inside of the bushing, the linkage groove is spirally located on the inside of the rotating sleeve, the linkage block is located on the outside of the linkage sleeve, and the movable block is connected to one side of the inclined plate. A locking mechanism is installed on the outside of the bushing, including a rotating plate, a rotating block, a movable spring, a fixed block, a rotating hole, a sliding rod, a sliding sleeve, a shifting spring, a shifting block, and a circular block. The movable spring is connected to the rotating block and the fixed block. The rotating hole is located on the rotating plate, the sliding rod is connected to one side of the sliding sleeve, the shifting block is located on one side of the rotating sleeve, and the circular block is installed on the outside of the bushing. This utility model solves the technical defects of traditional equipment in terms of operating efficiency, overload protection, and structural stability.

The invention relates to a construction method and application of an ANGPTL3 overexpression laying hen fatty liver hemorrhagic syndrome model, and the construction method comprises the following steps: (1) respectively integrating a transposon element carrying a chicken ANGPTL3 coding gene and a transposase gene into an adenovirus vector, and obtaining a recombinant adenovirus by using a virus packaging system; (2) punching the chick embryos developed for 4 days (E4) by adopting an equatorial plane windowing method; (3) introducing the recombinant adenovirus into an E4 chick embryo body in a microinjection manner; and (4) hatching to laying hens to prepare the laying hen fatty liver hemorrhagic syndrome model. The animal disease model is constructed on poultry through protein overexpression for the first time, and the laying hen fatty liver hemorrhagic syndrome model does not need to be fed with high-fat feed and has the advantages of being accurate in animal model construction, remarkable in fatty liver characterization and high in efficiency. The method has a great application value in research on the fatty liver hemorrhagic syndrome of laying hens and drug development and screening of poultry.

The invention relates to a chicken seasoning convenient for long-term preservation and a preparation method thereof. The chicken oil comprises refined chicken oil, dried chicken meat powder, Chinese yam powder, Chinese chestnut powder, Chinese wolfberry fruits, yeast extract powder, oyster sauce, chicken bone extract, disodium inosinate, disodium 5 '-ribonucleotide, glutamic acid, table salt, granulated sugar powder, turmeric powder, ginger juice, green onion juice, white pepper powder and maltodextrin. The chicken seasoning is in the form of a grease solid particle mixture, the whole chicken seasoning is in a pasty shape, and compared with existing powdery chicken seasoning, the pasty seasoning is easier to take quantitatively and does not have the problem of being affected with damp. And the grease has an effect of isolating air, so that even if the packaging bag is in an unsealed state, the packaging bag also has a relatively long shelf life.

This application relates to a large-particle dog and cat oral health functional food coated with quick-dissolving freeze-dried granules and its preparation method, belonging to the field of pet food technology. This formulation contains grain-based granules, an external spraying system consisting of 0.1-0.15 parts lecithin, 0.002-0.003 parts monoglycerides, 0.001-0.003 parts vitamin E, and 0.8-1.2 parts chicken fat, and an instant-dissolving freeze-dried microparticle layer consisting of 0.1-0.2 parts brown algae polysaccharide, 0.02-0.05 parts lysozyme, 0.04-0.08 parts tea polyphenols, 0.02-0.04 parts zinc gluconate, 0.05-0.1 parts gum arabic, 0.03-0.1 parts glutamine, 0.01-0.02 parts vitamin B2, 0.01-0.04 parts vitamin B12, and 0.02-0.07 parts glycine. Combined with large particles, it can stimulate saliva secretion, effectively inhibit the formation of dental plaque and tartar, and eliminate bad breath.

The invention discloses application of a dihydromyricetin and selenium composition in preparation of a medicine for treating chicken fatty liver hemorrhagic syndrome, and belongs to the technical field of medicine for animal husbandry. The application of the dihydromyricetin and selenium composition in the preparation of the medicine for treating the chicken fatty liver hemorrhagic syndrome is found in the invention; the mass ratio of the dihydromyricetin to the selenium is (1-5g): (50-100mu g); the selenium is derived from selenium-enriched spirulina powder; the composition of dihydromyricetin and selenium is added into a chicken basal feed for application. The addition amount of the dihydromyricetin in the chicken basal feed is 1-5g / kg, and the addition amount of the selenium in the chicken basal feed is 50-100mu g / kg. The combined action of dihydromyricetin and selenium can significantly relieve the chicken fatty liver hemorrhagic syndrome and laying rate reduction in the late laying period of the Xinghua chicken; a new medicine choice is provided for treating the chicken fatty liver hemorrhagic syndrome in the laying hen breeding process and prolonging the laying period of laying hens.

The invention discloses an adipogenesis induction culture medium suitable for natural immortal cells of chicken fat precursor cells, an adipogenesis induction method and application of the adipogenesis induction culture medium and the adipogenesis induction method, and belongs to the technical field of animal cell differentiation. The fat formation induction culture medium comprises a basic culture medium, fetal calf serum with the volume percentage being 0.1-2%, penicillin with the concentration being 100 U / mL, streptomycin with the concentration being 100 [mu] g / mL, OA with the concentration being 60-150 [mu] mol / L, EPA with the concentration being 40-80 [mu] mol / L, DHA with the concentration being 20-100 [mu] mol / L, insulin with the concentration being 5-10 [mu] g / mL, transferrin with the concentration being 10-20 [mu] g / mL and glutamine with the concentration being 2-4 mmol / L. The adipogenic differentiation effect of the adipogenic induction culture medium is good, the cell differentiation efficiency reaches 100%, the fat maturity is obviously improved, and the repeatability is good.

The application provides application of an IGFBP1 gene in regulating chicken fat deposition traits, and belongs to the technical fields of biotechnology and genetic breeding. The application constructs a tissue-specific IGFBP1 recombinant adeno-associated virus vector, and uses the tissue-specific IGFBP1 recombinant adeno-associated virus vector to promote expression of the IGFBP1 gene of a chicken, promotes expression of the IGFBP1 gene of the chicken by promoting expression of the IGFBP1 gene of the chicken, increases the content of IGFBP1 protein in serum of the chicken, and further increases the content of intramuscular fat of the chicken, reduces the weight of abdominal fat of the chicken and the abdominal fat rate of the chicken. Through long-term experimental research, it is found that the chicken IGFBP1 can significantly affect the differential deposition of chicken IMF and AbF, the expression level of serum IGFBP1 protein is significantly negatively correlated with the weight of abdominal fat and the abdominal fat rate of the chicken, and is significantly positively correlated with the content of intramuscular fat of the chicken, and can be used as a basis for a serum judgment index of chicken fat deposition traits, and can also be used for screening chicken breeds with high intramuscular fat and low abdominal fat rate.

The invention discloses liquid chicken oil and duck oil as well as a preparation method and application thereof in anti-liver cancer products, and relates to the technical field of oil fractionation, the liquid chicken oil or duck oil is heated to melt, cooled and crystallized, a surfactant and / or electrolyte aqueous solution is added when crystals appear, and the liquid chicken oil or the liquid duck oil is obtained through crystal growing, solid-liquid separation and oil-water separation; the surfactant and / or electrolyte are / is added in the oil fractionation process, so that the physicochemical properties of the chicken oil and the duck oil are improved, and the eating quality of the chicken oil and the duck oil is improved; pharmacological tests find that the prepared liquid chicken oil and liquid duck oil have certain inhibitory activity on liver cancer cells, and are expected to be applied to processing of anti-liver cancer foods or drugs, so that the economic values of the chicken oil and the duck oil are improved.