23 results about "Chicken fat" patented technology

The invention discloses a chicken myoblast and chicken fat precursor cell co-culture synchronous differentiation method and application thereof, and belongs to the technical field of animal cell co-culture and differentiation. The method comprises the following steps: respectively carrying out in-vitro multiplication culture on chicken myoblasts and chicken fat precursor cells, carrying out mixed co-culture, inducing for at most two days by adopting horse serum with the volume percent of 0.5-2%, carrying out pre-differentiation, and continuously carrying out induced differentiation culture for at most one day by using an induced adipogenesis culture medium. According to the synchronous differentiation method for co-culture of the chicken myoblasts and the chicken fat precursor cells, a cell co-culture system is constructed, time sequence induced differentiation is achieved, and a final product with fat droplets distributed in the gaps of muscle bundles (the average distance is 20-50 microns) and capable of recarving natural chicken intermuscular fat textures is obtained. According to the method, the differentiation period is shortened to be within 3 days from 21 days (the efficiency is improved by 600%); the induction cost is reduced by 42.7%; the final product breaks through the sensory acceptance barrier through bionic structure recarving.

The utility model relates to the technical field of chicken fat feed production, in particular to a presser convenient for residue cleaning for chicken fat feed production, which comprises a rack, a pressing roller is rotatably connected onto the rack, and a pressing filter screen cylinder with a residue outlet groove penetrating through the pressing roller is fixedly mounted in the pressing roller. One side of the squeezing roller is rotationally connected with a feeding pipe communicated with the squeezing filter screen cylinder, the feeding pipe is connected with the rack, a feeding cover is fixedly installed at the top of the end, away from the squeezing roller, of the feeding pipe, a scattering and conveying screw rod is rotationally connected into the feeding pipe, and a rotating frame is rotationally connected into the squeezing filter screen cylinder; according to the presser for chicken fat feed production, continuous feeding and pressing are facilitated, residues are synchronously scraped in the pressing process, pressing is convenient, and meanwhile the residues are convenient to clean.

The invention relates to a large-particle dog and cat oral health-care functional food wrapped with instant freeze-dried particles and a preparation method thereof, and belongs to the technical field of pet food. According to the scheme, an external spraying system comprises grain base material particles, 0.1 to 0.15 part of lecithin, 0.002 to 0.003 part of monostearin, 0.001 to 0.003 part of vitamin E and 0.8 to 1.2 parts of chicken oil, and an instant freeze-dried particle layer comprises 0.1 to 0.2 part of fucoidan, 0.02 to 0.05 part of lysozyme, 0.04 to 0.08 part of tea polyphenol, 0.02 to 0.04 part of zinc gluconate, 0.05 to 0.1 part of Arabic gum, 0.03 to 0.1 part of glutamine, 0.01 to 0.02 part of vitamin B2, 0.01 to 0.04 part of vitamin B12 and 0.02 to 0.07 part of glycine. The toothpaste can effectively inhibit the formation of dental plaque and dental calculus and remove halitosis.

FIELD: veterinary medicine and dietetics.SUBSTANCE: development of complete feed compositions that can provide a therapeutic effect in cases of exocrine pancreatic insufficiency. The composition of a complete dietary feed for cats with exocrine pancreatic insufficiency has been developed in the following ratio, wt.%: grain part: mixture of rice, wheat, hulled barley and corn – 37.0; protein part of plant origin: isolated soy protein, beet pulp, wheat bran, flax seeds – 22.0; protein part of animal origin: feather meal, chicken liver, egg powder – 25.0; mineral supplement: feed chalk, monocalcium phosphate, table salt, plantain seeds Plantago psyllium, mannanoligasaccharides with beta-glucans and lutein – 2.0; vitamin premix: A, D3, E, K, B1, B2, B3, B5, B6, B12, Bc, H, taurine, inulin, beech lignocellulose, complex of natural fulvic and humic acids – 2.5; flavor enhancer in the form of enzymatic hydrolysate of pork and chicken by-products – 3–3.5; brewer's yeast – 2.5–3.0; a mixture of fish and chicken fat – 5.0–6.0.EFFECT: obtaining food for cats with exocrine pancreatic insufficiency, which is easily reproducible, clinically effective, and convenient in dosing and use.1 cl, 3 tbl, 1 ex

The present application relates to a kind of chicken oil refining process, it is related to animal oil technology field, wherein the refining process includes pretreatment, low-temperature degumming, deacidification and decolorization, low-temperature deodorization, ceramic membrane filtration.The refining process of the present application is through the steps such as biological enzyme directional degumming, charged membrane separation deacidification and decolorization, nitrogen microbubble low-temperature deodorization, realizes the efficient refining of chicken oil at low temperature, so that the nutrient substance of chicken oil is not oxidized.

This invention relates to the field of feed additive technology, specifically to a feed additive that promotes digestion and absorption and its preparation process. The preparation process of a feed additive that promotes digestion and absorption includes: preparing antioxidant-modified chicken fat; preparing an extract; preparing a composite oil phase; and preparing the feed additive. In this invention, the extract of Shenqu (a type of fermented wheat) can directly compensate for insufficient endogenous enzyme secretion in laying hens, rapidly decomposing starch in feed and chicken fat in plant proteins, laying the foundation for subsequent absorption. The extract of Zhishi (a type of immature bitter orange) accelerates gastrointestinal motility and digesta emptying, while also promoting bile and pancreatic juice secretion, further improving the digestibility and utilization rate of fat-soluble components in laying hens. The extract of pine pollen can repair the intestinal mucosa, increase intestinal villus height, increase the nutrient absorption area, and regulate the intestinal microecology, reducing nutrient loss and intestinal stress. The combined use of these three ingredients synergistically promotes the digestive and absorptive capacity of laying hens.

The invention discloses a chicken body fat rate phenotype determination system and a chicken selection method. The chicken body fat rate phenotype determination system comprises an eight-electrode system, a multi-channel signal receiving and transmitting module, a processor module and a power supply module, the eight-electrode system is used for sending excitation current and receiving potential difference signals, and a local closed-loop measurement loop can be formed on the same limb; the multi-channel signal receiving and transmitting module is used for controlling excitation and measurement among the electrode groups and realizing biological impedance data acquisition in multiple directions; the processor module is used for constructing data models of the upper limbs, the trunk and the lower limbs of chicken body fat distribution according to the multi-channel impedance data; the power supply module is connected with the processor module and the eight-electrode system and provides power for the system. The method adopts the bioelectrical impedance value to measure and detect the body fat rate of the live chicken, and is high in detection precision, convenient to operate, high in adaptability and convenient to popularize and apply in the field of poultry breeding.

This invention discloses an enhancer that regulates chicken PGC-1α gene expression and its applications, belonging to the field of bioengineering. The nucleotide sequence of the enhancer is shown in SEQ ID NO: 1. This invention further discloses biomaterials containing this enhancer and a method for preparing cell lines with the enhancer knocked out. A dual-luciferase reporter gene system confirmed its significant activity in DF1 and ICP2 cells. Knocking out this enhancer downregulates PGC-1α gene expression in chicken preadipocytes, significantly promoting cell differentiation and lipid droplet formation. This enhancer can be used to prepare biological agents that regulate PGC-1α gene expression levels and chicken preadipocyte differentiation. It can also be applied to the genetic improvement of chicken fat traits and to the preparation of models for studying related gene function and cell differentiation mechanisms. This invention provides new targets and technical support for improving chicken fat traits.

The utility model relates to the technical field of residue scraping, in particular to a pool wall residue scraping device for a chicken oil sedimentation pool, which comprises a pool wall residue scraping machine, tightening assemblies are arranged on two sides of the pool wall residue scraping machine, and a height adjusting assembly is mounted below the pool wall residue scraping machine. And the tightening assembly comprises a lead screw, a guide block is fixedly installed below the lead screw, the outer wall of the lead screw is connected with a nut pair, and a rotating rudder is fixedly arranged on the outer wall of the nut pair. According to the pool wall slag scraping device, due to the arrangement of the tightening assembly, the toothed chain of the pool wall slag scraping machine can be tightened, operation is easy and fast, it can be guaranteed that the scraping plate of the pool wall slag scraping machine effectively scrapes oil residues, efficiency is improved, due to the arrangement of the height adjusting assembly, the height adjusting plate is located at the same height as chicken oil, and the oil residue scraping efficiency is improved. Bolts penetrate through holes of the fixing plate and the limiting plate to be tightened, the position of the height adjusting plate is fixed, the position of the height adjusting plate is conveniently adjusted, and then the chicken oil residues of different heights are cleaned.

The invention discloses an enhancer for regulating and controlling chicken PGC-1alpha gene expression and application of the enhancer, and belongs to the field of bioengineering. The nucleotide sequence of the enhancer is shown as SEQ ID NO: 1. The invention further discloses a biological material containing the enhancer and a preparation method of a cell strain with the enhancer knocked out. A dual-luciferase reporter gene system proves that the enhancer has remarkable activity in DF1 and ICP2 cells, the expression of the PGC-1alpha gene in chicken preadipocytes can be reduced by knocking out the enhancer, and cell differentiation and lipid droplet generation are remarkably promoted. The enhancer can be used for preparing a biological preparation for regulating and controlling the expression level of the PGC-1alpha gene and regulating and controlling chicken preadipocyte differentiation, can also be applied to genetic improvement of chicken fat traits and can be used for preparing a model for researching related gene functions and cell differentiation mechanisms. The invention provides a new target and technical support for chicken fat character improvement.

The invention discloses a method for improving eating and safety quality of salt roasted chicken based on low-temperature plasma and EGCG (epigallocatechin gallate) treatment. The preparation method comprises the following steps: by taking fresh chicken as a raw material, cleaning, removing hair, removing fat and fascia, pickling with salt and cooking wine, soaking in prepared marinating soup for marinating, treating with dielectric barrier discharge type low-temperature plasma equipment and soaking with an EGCG (epigallocatechin gallate) solution, and carrying out vacuum packaging and cold storage. The electric field discharge frequency of the dielectric barrier discharge type low-temperature plasma equipment is controlled to be 25-35 kHz, and the discharge voltage is controlled to be 40-60 kV; an EGCG aqueous solution with the mass concentration of 0.05-0.15% is controlled to be adopted in the EGCG soaking treatment, and the soaking time is 10-15 min. According to the invention, the shelf life of the salt-roasted chicken can be prolonged by 14 days, fat oxidation and volatile basic nitrogen accumulation of the salt-roasted chicken are delayed, the color and texture of the salt-roasted chicken are maintained, and the comprehensive commodity value of the salt-roasted chicken is improved.

The invention relates to the technical field of poultry breeding, and particularly provides a novel application of miR-363-5p. A hepatocyte model is established through overexpression and knock-down of the miR-363-5p so as to detect key indexes related to lipid synthesis, oxidative stress and inflammatory factors, and research results show that inflammatory response in chicken hepatocytes can be inhibited, oxidative stress can be reduced and lipid degradation can be prevented by reducing expression of the miR-363-5p. In addition, the invention also finds that the development of chicken liver cells is controlled by targeting the miR-363-5p to the CPEB2, ERK signal transduction can be activated by knocking down the miR-363-5p or overexpressing the CPEB2, so that the miR-363-5p-CPEB2-MAPK / ERK axis is determined to be a key regulatory factor in the pathogenesis of the chicken fatty liver. Therefore, the miR-363-5p and / or CPEB2 gene can be used as a detection target to develop a reagent or a kit for diagnosing the chicken fatty liver syndrome.

The application discloses a chicken oil storage tank, and relates to the technical field of oil storage tanks, which comprises a tank body, and a supporting base is fixedly installed around the bottom of the tank body; the stirring paddle is continuously rotated to stir the chicken oil stored in the tank body, the rotation of the stirring paddle can fully mix the chicken oil in different parts of the tank, the rotation of the stirring paddle can fully mix the chicken oil in different parts of the tank, continuous stirring can fully contact the chicken oil with air, accelerate the consumption and update of oxygen in the chicken oil, reduce the oxidation reaction caused by the excessively high local oxygen concentration, in addition, the rotation of the stirring paddle can keep the chicken oil in a constant motion state, reduce the residence time and adhesion opportunity of the chicken oil on the tank wall, thereby preventing the chicken oil from forming a thick deposition layer on the tank wall and reducing the risk of tank wall corrosion caused by long-term adhesion.

This utility model discloses a high-efficiency pressing device for producing chicken oil as a feed additive, including a base on which a pressing device is mounted. A coupling device is also provided on the base. The coupling device includes a bushing, a shaft, a rotating sleeve, a slot, an inclined groove, an inclined plate, a linkage groove, a linkage sleeve, a linkage block, and a movable block. The slot is located on the outside of the shaft, the inclined groove is located on the inside of the bushing, the linkage groove is spirally located on the inside of the rotating sleeve, the linkage block is located on the outside of the linkage sleeve, and the movable block is connected to one side of the inclined plate. A locking mechanism is installed on the outside of the bushing, including a rotating plate, a rotating block, a movable spring, a fixed block, a rotating hole, a sliding rod, a sliding sleeve, a shifting spring, a shifting block, and a circular block. The movable spring is connected to the rotating block and the fixed block. The rotating hole is located on the rotating plate, the sliding rod is connected to one side of the sliding sleeve, the shifting block is located on one side of the rotating sleeve, and the circular block is installed on the outside of the bushing. This utility model solves the technical defects of traditional equipment in terms of operating efficiency, overload protection, and structural stability.

This application relates to a large-particle dog and cat oral health functional food coated with quick-dissolving freeze-dried granules and its preparation method, belonging to the field of pet food technology. This formulation contains grain-based granules, an external spraying system consisting of 0.1-0.15 parts lecithin, 0.002-0.003 parts monoglycerides, 0.001-0.003 parts vitamin E, and 0.8-1.2 parts chicken fat, and an instant-dissolving freeze-dried microparticle layer consisting of 0.1-0.2 parts brown algae polysaccharide, 0.02-0.05 parts lysozyme, 0.04-0.08 parts tea polyphenols, 0.02-0.04 parts zinc gluconate, 0.05-0.1 parts gum arabic, 0.03-0.1 parts glutamine, 0.01-0.02 parts vitamin B2, 0.01-0.04 parts vitamin B12, and 0.02-0.07 parts glycine. Combined with large particles, it can stimulate saliva secretion, effectively inhibit the formation of dental plaque and tartar, and eliminate bad breath.

The invention discloses an adipogenesis induction culture medium suitable for natural immortal cells of chicken fat precursor cells, an adipogenesis induction method and application of the adipogenesis induction culture medium and the adipogenesis induction method, and belongs to the technical field of animal cell differentiation. The fat formation induction culture medium comprises a basic culture medium, fetal calf serum with the volume percentage being 0.1-2%, penicillin with the concentration being 100 U / mL, streptomycin with the concentration being 100 [mu] g / mL, OA with the concentration being 60-150 [mu] mol / L, EPA with the concentration being 40-80 [mu] mol / L, DHA with the concentration being 20-100 [mu] mol / L, insulin with the concentration being 5-10 [mu] g / mL, transferrin with the concentration being 10-20 [mu] g / mL and glutamine with the concentration being 2-4 mmol / L. The adipogenic differentiation effect of the adipogenic induction culture medium is good, the cell differentiation efficiency reaches 100%, the fat maturity is obviously improved, and the repeatability is good.

The application provides application of an IGFBP1 gene in regulating chicken fat deposition traits, and belongs to the technical fields of biotechnology and genetic breeding. The application constructs a tissue-specific IGFBP1 recombinant adeno-associated virus vector, and uses the tissue-specific IGFBP1 recombinant adeno-associated virus vector to promote expression of the IGFBP1 gene of a chicken, promotes expression of the IGFBP1 gene of the chicken by promoting expression of the IGFBP1 gene of the chicken, increases the content of IGFBP1 protein in serum of the chicken, and further increases the content of intramuscular fat of the chicken, reduces the weight of abdominal fat of the chicken and the abdominal fat rate of the chicken. Through long-term experimental research, it is found that the chicken IGFBP1 can significantly affect the differential deposition of chicken IMF and AbF, the expression level of serum IGFBP1 protein is significantly negatively correlated with the weight of abdominal fat and the abdominal fat rate of the chicken, and is significantly positively correlated with the content of intramuscular fat of the chicken, and can be used as a basis for a serum judgment index of chicken fat deposition traits, and can also be used for screening chicken breeds with high intramuscular fat and low abdominal fat rate.

The invention belongs to the technical field of cell culture, and particularly relates to a method for establishing chicken primary hepatocyte lipid droplet induction and application, and the method comprises the following steps: S1, separating and culturing chicken primary hepatocytes; s2, carrying out induced culture on the chicken primary hepatocytes obtained in the step S1 by using an induced differentiation culture medium containing phosphatidyl ethanolamine so as to promote the formation of lipid droplets in the cells; s3, detecting the lipid droplet induction effect of the chicken liver cells; the invention provides a scheme for replacing traditional oleic acid or palmitic acid with phosphatidyl ethanolamine as a chicken primary hepatocyte lipid droplet induction reagent, provides an in-vitro research model for simulating the induction of the chicken fatty liver syndrome by phosphatidyl ethanolamine, and has important significance on the research of the comprehensive pathogenesis of the chicken fatty liver.

The application relates to the field of poultry breeding technology, and specifically provides a new application of miR-363-5p. A liver cell model is established by overexpressing and knocking down miR-363-5p to detect key indexes related to lipid synthesis, oxidative stress and inflammatory factors, and the research results show that the inflammatory reaction in chicken liver cells can be inhibited, oxidative stress can be reduced, and lipid degradation can be prevented by reducing the expression of miR-363-5p. In addition, it is found that miR-363-5p targets CPEB2 to control the development of chicken liver cells, and the ERK signal transduction can be activated by knocking down miR-363-5p or overexpressing CPEB2, and it is established that the miR-363-5p-CPEB2-MAPK / ERK axis is a key regulatory factor in the pathogenesis of chicken fatty liver. Therefore, the miR-363-5p and / or CPEB2 gene can be used as a detection target to develop a reagent or kit for diagnosing chicken fatty liver syndrome.

The present application relates to the technical field of animal feed, and particularly relates to a weight control prescription pet food for dogs and a preparation method, which is prepared from the following components in parts by weight: chicken meal 30-40 parts, cassava 10-15 parts, sweet potato 4-8 parts, chicken fat 1-5 parts, fish oil 1-5 parts, pea fiber 20-40 parts, flaxseed 1-4 parts, lactic acid 0.1-0.5 parts, chicken liver powder 1-5 parts, chicory 2-4 parts, coconut oil 0.3-0.7 parts, carrot 0.5-1.5 parts, potassium chloride 0.1-0.3 parts, DL-methionine 0.3-0.7 parts, salt 0.3-0.7 parts, fructooligosaccharide 0.3-0.7 parts, L-carnitine 0.1-0.5 parts, and premix 1-2 parts. The present application can improve the metabolic health of obese dogs, relieve and prevent chronic low-grade inflammation, insulin resistance, oxidative damage and other problems by precisely controlling the nutritional components such as protein level, fatty acid composition, dietary fiber and functional additives in the pet dog food, and effectively intervening the fat metabolism and inflammatory state by using L-carnitine, prebiotics and antioxidants.

The invention discloses a production process of chicken essence without addition of starch. The preparation method comprises the following steps: 1, pretreating raw materials, namely performing enzymolysis on minced chicken to generate small molecular peptides and amino acids, concentrating into paste, and then refining chicken oil to remove impurities and retain fragrance; 2, homogenizing and mixing the mixed solution of the enzymatic hydrolysate and the chicken oil with the solid raw materials in a stirring tank by adopting a wet mixing method to avoid starch dependence; 3, granulation and drying are carried out on the basis of the step 2, specifically, the mixed slurry is subjected to spray drying through a low-temperature spray drying method to form fine particles, starch adhesion is not needed, in addition, fluidized bed granulation is utilized, powder is suspended through airflow, an adhesive is sprayed, uniform particles are formed, and 0.1-0.5% of microcrystalline cellulose is added as a flow promoter; and step 4, after drying, rapidly cooling the particles to room temperature. Compared with the prior art, the preparation method has the advantages that the dissolution rate of the product is increased by 40%, the content of umami substances is increased by 25%, and thus the shelf life is prolonged.

The invention relates to freshness-enhancing microcapsule chicken powder and a preparation method thereof.The freshness-enhancing microcapsule chicken powder comprises a core material and a wall material wrapping the outer layer of the core material, and the core material comprises chicken powder, chicken oil, sodium glutamate, edible salt, white granulated sugar, radix angelicae / dried ginger spice oil, disodium 5 '-ribonucleotide, soybean protein isolate, a yeast extract, disodium succinate, carotene and Omega-3 fatty acid; and the wall material comprises the following raw materials: starch sodium octenylsuccinate, maltodextrin and beewax. Chicken powder is prepared into a capsule shape, fragrant substances are slowly released through embedding of a wall material, and the flavor is lasting. Raw materials of a wall material are improved, the starch sodium alkenyl succinate, maltodextrin and beeswax are adopted as raw materials to form the wall material, the porosity of the surface of the wall material is smaller than or equal to 1.5%, oxidative deterioration of active substances is reduced, the hardness of the wall material can be improved by adding the beeswax, and the stability of the microcapsule chicken powder is improved; omega-3 fatty acid which is easy to oxidize is added into the chicken powder, so that the Omega-3 fatty acid can be provided in daily life, and the nutritional value of the chicken powder is improved; omega-3 fatty acid is subjected to nano emulsification pretreatment, so that oxidation of the Omega-3 fatty acid can be avoided.

The invention discloses an lncRNA gene molecular marker related to fat traits of Huainan yellow chickens and application of the lncRNA gene molecular marker, and belongs to the technical field of molecular marker-assisted breeding. The lncRNA gene molecular marker related to the Huainan yellow chicken fat traits is obtained through screening and located in an intron region of a chicken chromosome 1 lncRNA gene, the nucleotide sequence of the lncRNA gene molecular marker is shown as SEQ ID NO.1, and a C / T polymorphic site exists at the 284bp position of the sequence. The invention also develops a primer group for detecting the molecular marker and prepares a detection product containing the primer, and the primer group can accurately identify the relationship between the SNP marker and the local Huainan yellow chicken fat related traits, so as to provide a basis for local Huainan yellow chicken variety breeding and marker-assisted selection.