Molecular signing method for predicting and identifying chicken body fat character

A molecular marker and chicken body fat technology, applied in the field of animal molecular genetics, can solve the problems that the heart cannot effectively absorb long-chain fatty acids, the loss cannot be fully compensated, and the tolerance is reduced, achieving high accuracy and low cost , The effect of accelerating the breeding process

Inactive Publication Date: 2006-03-29
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Among them, the H-FABP gene knockout mice showed severe defects in the utilization of peripheral long-chain fatty acids, and the heart could not effectively uptake the long-chain fatty acids in plasma as the main fuel under normal conditions, and began to utilize glucose (Binas et al. , 1999)
Loss of H-FABP cannot be fully compensated and leads to reduced tolerance to strenuous exercise, leading to localized myocardial hypertrophy in the elderly

Method used

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  • Molecular signing method for predicting and identifying chicken body fat character

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] Embodiment 1, the polymorphism of chicken H-FABP gene and F 2 Correlation of Abdominal Fat Traits in Resource Groups

[0115] Utilize primers (HFF7 and HFR7) of the present invention to F 2 The genomic DNA of 564 individuals in the resource population was amplified by PCR, and then analyzed by polyacrylamide gel electrophoresis. in F 2 A total of 3 genotypes were detected in the resource population. For individuals with a PCR amplified fragment length of 181 bp, they were named AA genotype; for individuals with a PCR amplified fragment length of 195 bp, they were named BB genotype; The individuals whose amplified products are two bands (181bp and 195bp) are named AB genotype (figure).

[0116] The 3 genotypes and F 2 The least squares analysis was performed on the abdominal fat weight and abdominal fat rate of 564 individuals in the resource group, and the results showed that the genotype had a significant effect on F 2 Abdominal fat weight and abdominal fat rate o...

Embodiment 2

[0134] Example 2. Correlation between polymorphisms of chicken H-FABP gene and abdominal fat traits of the sixth generation of broiler high and low fat bidirectional selection lines

[0135] The primers (HFF7 and HFR7) of the present invention are used to carry out PCR amplification on the genomic DNA of the sixth generation of broiler high-low-fat bidirectional selection line, and then perform polyacrylamide gel electrophoresis analysis. A total of 3 genotypes were detected in the sixth generation. For individuals with a PCR amplification fragment length of 181bp, they were named AA genotype; for individuals with a PCR amplification fragment length of 195bp, they were named BB genotype; For individuals with two bands (181bp and 195bp) in the PCR amplification product, they were named as AB genotype.

[0136] The least squares analysis was performed on the three genotypes of the 14bp insertion / deletion variation site of the H-FABP gene and the abdominal fat weight and abdomina...

Embodiment 3

[0145] Example 3. Correlation between polymorphism of chicken H-FABP gene and abdominal fat traits of the seventh generation of broiler high and low fat bidirectional selection lines

[0146] The primers (HFF7 and HFR7) of the present invention are used to perform PCR amplification on the genomic DNA of the seventh generation of broiler high-low-fat bidirectional selection line, and then perform polyacrylamide gel electrophoresis analysis. A total of 3 genotypes were detected in the seventh generation. For individuals with a PCR amplification fragment length of 181bp, they were named AA genotype; for individuals with a PCR amplification fragment length of 195bp, they were named BB genotype; For individuals with two bands (181bp and 195bp) in the PCR amplification product, they were named as AB genotype.

[0147] The least squares analysis was performed on the three genotypes of the 14bp insertion / deletion variation site of the H-FABP gene and the abdominal fat weight and abdom...

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Abstract

A molecular marker method for predicting and determining the characteristics of chicken fat includes such steps as designing a pair of primers according to the H-FABP gene sequence of chicken, PCR amplifying of the chicken genomic DNA, electrophoretic separation of PCR product by polyacrylamide gel, detecting the variant sites of 14 bp insertion / deletion in the intron II of H-FABP gene, and naming. Its advantages are simple operation, low cost and high precision.

Description

[0001] (1) Fields [0002] The invention belongs to the field of animal molecular genetics, in particular to a method for detecting chicken body fat traits with molecular markers. (two), background technology [0003] Excessive accumulation of body fat (especially abdominal fat) in fast and large broilers has become a prominent problem. Excessive fat deposition in broilers has many disadvantages: (1) significantly reduces the feed conversion efficiency, because the deposition of adipose tissue per unit weight consumes three times more energy than the deposition of lean meat per unit weight; (2) reduces the amount of carcass lean meat. (3) Processors and consumers use a large portion of these fats deposited in broilers (abdominal fat pad, gizzard fat, crop fat and mesenteric fat, etc.) ) is discarded, which not only increases the burden on processors and consumers, but also increases the fat content in waste and treated water, thereby polluting the environment. From this poin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 李辉王启贵
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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