Breeding method of maize haploid inducing line and special primer thereof

A haploid induction system and haploid induction technology, applied in the field of molecular genetics breeding, can solve the time-consuming and labor-intensive problems of haploid induction rate statistics, and achieve the effects of increasing the breeding probability, accelerating the process, and improving efficiency

Active Publication Date: 2015-08-19
CHINA AGRI UNIV
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Problems solved by technology

Each single plant needs 3-5 test fruit ears to objectively reflect the induction rate level of the tested single plant. After obtaining the induced fruit ears, each seed needs to be screened, and

Method used

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  • Breeding method of maize haploid inducing line and special primer thereof
  • Breeding method of maize haploid inducing line and special primer thereof
  • Breeding method of maize haploid inducing line and special primer thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0031] Example 1. Acquisition and application of molecular markers

[0032] 1. Discovery and fine mapping of haploid-induced traits (HIR) qHI-8

[0033] Use four Fs 2 The population performed a genome-wide scan for the trait of induction rate and found 8 QTL loci that control HIR. The QTL located on chromosome 9 is named qHI-8, which is in addition to qHI-1 located on chromosome 1. The most effective QTL.

[0034] 2. Acquisition of molecular markers

[0035] 1. F 2 Group development

[0036] With CAUHOI and UH400 as parents, they were sown at the Beijing Shangzhuang Experimental Station at the same time. During the pollination period, the two materials were pollinated to obtain F 1 seed. In the winter of the same year, F 1 The seeds were planted in the base of Hainan Nanfan Company, and obtained F after selfing 2 Group, will F 2 Seeds were planted in Beijing and used for molecular marker screening. Single plants carrying qHI-8 sites were selected and F was obtained after selfing. 3 gr...

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Abstract

The invention discloses a molecular-marker-assisted breeding method of a maize haploid inducing line and a special primer thereof. The primer is composed of a DNA (deoxyribonucleic acid) molecule disclosed as SEQ ID NO.1 and a DNA molecule disclosed as SEQ ID NO.2. The test proves that the molecular-marker-assisted breeding method of a maize haploid inducing line can directly identify a single plant containing two main effect QTLs (quantitative trait loci) qHI-1 and qHI-8, eliminate the other genotypes and quickly obtain the two-locus homozygosis plant line, thereby saving abundant unnecessary examination and haploid selection work, obviously enhancing the haploid inducing line breeding efficiency, accelerating the haploid inducing line breeding progress and increasing the breeding probability of favorable inducing lines.

Description

technical field [0001] The invention relates to a breeding method of a maize haploid induction line and special primers thereof, belonging to the field of molecular genetics breeding. Background technique [0002] Corn is the largest food crop in my country (Zhang Chunlei, 2012), and it is also one of the most important energy crops in the world. The increase of corn production is of great significance to meet the supply of edible feed and ensure national food security. Under the condition of limited land resources today, increasing the yield per unit area of ​​corn is of great significance to ensure the growth of total corn production. [0003] Improved seed is one of the important elements to increase maize yield. Excellent varieties need the support of excellent inbred lines. In the current breeding technology of new varieties, the breeding of excellent inbred lines is the core content. However, in traditional breeding, it takes 6-8 generations for the selection of in...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 陈绍江刘晨旭钟裕
Owner CHINA AGRI UNIV
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