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Breeding method of maize haploid inducing line and special primer thereof

A haploid induction system and haploid induction technology, applied in the field of molecular genetics breeding, can solve the time-consuming and labor-intensive problems of haploid induction rate statistics, and achieve the effects of increasing the breeding probability, accelerating the process, and improving efficiency

Active Publication Date: 2015-08-19
CHINA AGRI UNIV
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Problems solved by technology

Each single plant needs 3-5 test fruit ears to objectively reflect the induction rate level of the tested single plant. After obtaining the induced fruit ears, each seed needs to be screened, and haploid grains and heterozygous grains are separated, and the Counting is used to calculate the induction rate, and the statistics of the haploid induction rate are time-consuming and laborious

Method used

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  • Breeding method of maize haploid inducing line and special primer thereof
  • Breeding method of maize haploid inducing line and special primer thereof
  • Breeding method of maize haploid inducing line and special primer thereof

Examples

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Embodiment 1

[0031] Example 1. Obtaining and Application of Molecular Markers

[0032] 1. Discovery and fine mapping of qHI-8 for haploid-induced traits (HIR)

[0033] Use the four F's 2 The population conducted a genome-wide scan for the trait of induction rate, and found 8 QTL loci controlling HIR, among which the QTL located on chromosome 9 was named qHI-8, which was in addition to qHI-1 located on chromosome 1 QTL with the largest effect.

[0034] 2. Acquisition of Molecular Markers

[0035] 1. F 2 group development

[0036] Taking CAUHOI and UH400 as parents, they were sown at the Shangzhuang Experimental Station in Beijing at the same time, and the two materials were pollinated with each other during the loose pollination period to obtain F 1 seed. In the winter of the same year, the F 1 The seeds were planted in the base of Hainan Nanfan Company, and F 2 groups, the F 2 Seeds were planted in Beijing and used for molecular marker screening. Individual plants carrying the qHI-8...

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Abstract

The invention discloses a molecular-marker-assisted breeding method of a maize haploid inducing line and a special primer thereof. The primer is composed of a DNA (deoxyribonucleic acid) molecule disclosed as SEQ ID NO.1 and a DNA molecule disclosed as SEQ ID NO.2. The test proves that the molecular-marker-assisted breeding method of a maize haploid inducing line can directly identify a single plant containing two main effect QTLs (quantitative trait loci) qHI-1 and qHI-8, eliminate the other genotypes and quickly obtain the two-locus homozygosis plant line, thereby saving abundant unnecessary examination and haploid selection work, obviously enhancing the haploid inducing line breeding efficiency, accelerating the haploid inducing line breeding progress and increasing the breeding probability of favorable inducing lines.

Description

technical field [0001] The invention relates to a breeding method of a maize haploid induction line and special primers thereof, belonging to the field of molecular genetics breeding. Background technique [0002] Corn is the largest food crop in my country (Zhang Chunlei, 2012), and it is also one of the most important energy crops in the world. The increase of corn production is of great significance to meet the supply of edible feed and ensure national food security. Under the condition of limited land resources today, increasing the yield per unit area of ​​corn is of great significance to ensure the growth of total corn production. [0003] Improved seed is one of the important elements to increase maize yield. Excellent varieties need the support of excellent inbred lines. In the current breeding technology of new varieties, the breeding of excellent inbred lines is the core content. However, in traditional breeding, it takes 6-8 generations for the selection of in...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6895C12Q2600/13
Inventor 陈绍江刘晨旭钟裕
Owner CHINA AGRI UNIV
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