509 results about "Gene silencing" patented technology

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses a method for improving citrus yellow vein disease resistance by using a ClPsaN gene. The method comprises the following steps: (1) cloning a VIGS fragment of the citrus ClPsaN gene; (2) constructing two VIGS expression vectors of TRV and CLBV; and (3) transforming the citrus by the VIGS expression vector to obtain a citrus ClPsaN gene silenced VIGS plant. According to the invention, the VIGS vector of the Citrus ClPsaN gene is constructed, and then the Citrus is transformed, so that the obtained Citrus plant can show obvious resistance to the Citrus yellow vein clearing virus, the titer of the Citrus yellow vein clearing virus can be reduced by 22-34%, the accumulation amount of the virus protein is reduced by 35-50%, and the attack degree of the Citrus yellow vein clearing virus is obviously reduced; the TRV silencing of the citrus ClPsaN gene does not influence the phenotype of a citrus plant, and the citrus ClPsaN gene can be used as a candidate gene for citrus yellow vein disease resistance breeding together with a plurality of citrus yellow vein disease-resistant and susceptible genes by utilizing the cooperation of VIGS silencing, RNA interference, gene editing and other technologies.

The invention discloses an RcWRKY29 gene and application of the RcWRKY29 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY29 gene is shown as SEQ ID NO: 1. Researches find that the gene is specifically and highly expressed in Chinese rose petals, and the expression quantity is remarkably increased within 24-48 hours after botrytis cinerea infection. After RcWRKY29 expression is instantaneously inhibited through a virus-induced gene silencing technology, the scab area of Chinese rose petals is increased, and the disease resistance is remarkably reduced; otherwise, overexpression of the gene can significantly enhance disease resistance and reduce scab area and cell damage. The invention provides a recombinant vector containing RcWRKY29, an engineering bacterium and a detection kit, and develops a method for enhancing the disease resistance of Chinese rose based on an agrobacterium-mediated transformation technology. The invention provides a new target for Chinese rose gray mold resistance molecular breeding, can effectively reduce postharvest disease loss, and has important economic value and popularization and application prospects.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms, dsRNA and application thereof. Specifically, the nucleotide sequences of the three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms are respectively SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The recombinant Hc beta GRP protein is successfully obtained through a prokaryotic expression system, an in-vitro bacteriostatic test proves that the recombinant Hc beta GRP protein, the Hc beta GRP protein and the dsRNA have direct bacteriostatic activity, dsRNA is designed based on a pattern recognition protein Hc beta GRP gene, a hyphantria cunea Hc beta GRP dsRNA interference system implemented by using an injection method is established, an obvious gene silencing effect is obtained by injecting the Hc beta GRP dsRNA interference system into hyphantria cunea, and the Hc beta GRP dsRNA interference system is applied to the hyphantria cunea. The technical support is provided for the application of the Hc beta GRP RNAi technology. Besides, the dsRNA of the hyphantria cunea Hc beta GRP is combined with the biocontrol bacteria by utilizing an established hyphantria cunea Hc beta GRP RNA interference system, so that the control effect of the biocontrol bacteria can be remarkably improved.

The invention discloses an RGD-click chemical crosslinking siRNA nano-carrier, a preparation method thereof and application of the nano-carrier in preparation of a medicine for treating secondary thyroidism, and belongs to the technical field of medicines.The nano-carrier is RGD-PEG-PLys (N), and the preparation method of the nano-carrier comprises the steps of synthesis of PLys (ss-DBCO), synthesis of RGD-PEG-PLys (N) and the like. According to the application, the nano-carrier is used for preparing siRNA composite nanoparticles; vascular endothelial targeting (RGD), dynamic stability (click crosslinking) and microenvironment responsiveness (disulfide bond) are organically combined for the first time to achieve mutual synergistic interaction, and the effect ceiling of an existing material is broken through; according to the siRNA composite nanoparticle, the silence effect of the PTH gene as long as 70 days can be achieved through single intravenous injection, the PTH inhibition rate is larger than 85%, the siRNA accumulation amount in parathyroid gland tissue is remarkably increased through RGD targeting (8.6 times that of a non-targeting group), the liver and kidney distribution amount is reduced by 60%, and the system toxicity is controllable.

The invention belongs to the technical field of cytotoxicity intervention, and particularly relates to application of an iNOS inhibitor in preparation of a medicine for relieving cellular injury caused by toxin combined exposure. KK-1 cells serve as a research model, it is shown for the first time that MC-LR and NaNOS combined contamination can remarkably up-regulate the expression level of iNOS protein, and it is found through tests that Nos2 gene silencing can remarkably relieve tight junction damage and cell apoptosis caused by toxin combined exposure in the KK-1 cells; it is revealed for the first time that iNOS is a core target of toxin combined exposure induced cell tight junction damage and apoptosis, and the blank of toxic mechanism research in toxin combined exposure is filled. The application provides a new direction for a detoxification strategy of environmental toxin combined exposure, provides a theoretical basis for developing an iNOS-targeted inhibitor, and also provides a new target for etiological recognition, early diagnosis and prevention of related diseases caused by environmental toxin combined exposure.

The invention relates to the technical field of biology, in particular to a vitis davidii VdYUCCA10 gene as well as an encoding protein and application thereof. The vitis davidii VdYUCCA10 gene is located on a chromosome 7 of vitis davidii and is distributed in a 2271185-2274940 region, and the nucleotide sequence of the vitis davidii VdYUCCA10 gene is shown as SEQ ID NO. 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID No.2. The invention also discloses an application of the vitis davidii VdYUCCA10 gene in enhancing the resistance of grape fruits to pathogenic bacteria after silencing. After the VdYUCCA10 gene provided by the invention is silenced, the resistance of grapes to anthracnose can be improved, and a theoretical basis is provided for grape anthracnose resistance breeding.

The invention provides an hpRNA interference vector for laver and application of the hpRNA interference vector, and belongs to the technical field of genetic engineering and algae biology. According to the hpRNA interference vector, a binary vector pBI121 is used as a skeleton vector, a laver endogenous Actin promoter and a multiple cloning site segment are inserted, the multiple cloning sites allow target gene segments to be cloned in a forward and reverse repetition mode, the target gene segments are spaced by introns, and therefore an efficient hpRNA structure is formed after transcription, and the efficient hpRNA interference vector is obtained. The gene can be directly used for silencing important genes related to growth and development, metabolic regulation, stress response and the like in the laver. The invention further provides a construction method of the hpRNA interference vector and application of the hpRNA interference vector in silencing laver genes and provides a method for silencing laver gene expression, the gene silencing effect is remarkable, experimental results can be repeated, and a powerful tool is provided for molecular breeding and genetic improvement of laver.

The invention discloses a gene RhNADP-LIKE for regulating and controlling Chinese rose flower aging, and particularly relates to the technical field of molecular biology and horticultural plant physiology. According to the invention, bottle insertion life phenotype data of 358 parts of core germplasm of tetraploid Chinese rose is screened through genome-wide association analysis (GWAS), and the gene RhNADP-LIKE significantly associated with senescence is identified. The gene accelerates the aging process of petals by regulating the accumulation of reactive oxygen species (ROS). After RhNADP-LIKE expression is inhibited by using a virus-induced gene silencing (VIGS) technology, the blooming period of rosa chinensis flowers is prolonged by about 2 days, and the ROS level is remarkably reduced. The invention provides a molecular target for prolonging the vase life of the Chinese rose, can be applied to gene editing breeding or development of an environment-friendly preservative, reduces the dependence of chemical agents, and improves the economic benefit and sustainable development potential of the cut flower industry.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention provides application of a TaNAC42 gene in regulation and control of wheat stem rust resistance, and belongs to the technical field of gene functions. It is found for the first time that the TaNAC42 gene of wheat is related to the wheat stem rust resistance, the TaNAC42 gene is induced to be silenced through viruses, the wheat stem rust resistance can be enhanced, wheat plants are easier to resist diseases, the TaNAC42 gene is over-expressed through the agrobacterium tumefaciens-mediated transient over-expression technology, the wheat stem rust resistance can be weakened, the wheat plants are more susceptible to diseases, and the wheat plants are more susceptible to diseases. Namely, the TaNAC42 gene can negatively participate in regulating and controlling the resistance of the wheat to the Puccinia graminicola, a new target gene is provided for improved breeding of wheat Puccinia graminicola resistance molecules, and the TaNAC42 gene has important utilization value in wheat production and breeding.

The invention discloses a VIGS silencing system of walnut JrPDS gene as well as a construction method and application of the VIGS silencing system, and relates to the technical field of biology. The walnut JrPDS gene interference fragment as shown in SEQ ID NO.1 provided by the invention can effectively interfere the expression of the walnut endogenous JrPDS gene, so that the walnut seedling endogenous JrPDS gene is silenced, the silencing rate can reach 48%, and the walnut JrPDS gene interference fragment is used for identifying the function of the walnut endogenous gene.

The application discloses a kind of carrier, kit and application for long-acting gene knockdown of animal parasitic nematode, belong to the field of animal parasitic disease prevention and control.The lentivirus vector in the application integrates specific coding sequence to the genome of the infected nematode, stably and continuously expresses short hairpin RNA (shRNA) in the nematode, efficiently produces primary single-stranded small RNA (siRNA), these siRNAs target homologous target gene mRNA to cause its degradation, realize stable and continuous gene silencing effect.The application breaks through the bottleneck of traditional animal parasitic nematode RNA interference (RNAi) technology with low efficiency and instability, significantly improves the timeliness of conventional RNAi, can be used to establish long-acting RNAi technology system of animal parasitic nematode, has application value in the research of host in vivo process such as nematode infection, parasitism and pathogenicity and animal parasitic nematode disease prevention and control.

The invention discloses an application of a tomato Solyc04g064880 gene in regulation and control of ToBRFV resistance of a plant, a Solyc04g064880 gene silencing vector pTRV2 is constructed: S14880, agrobacterium tumefaciens is transformed, positive bacteria obtained by screening are inoculated into a target plant to obtain a Solyc04g064880 gene silencing strain, and compared with a contrast, the Solyc04g064880 gene silencing strain has the advantages that the ToBRFV accumulation amount in the plant is reduced, and the ToBRFV resistance of the plant can be regulated and controlled. Therefore, the silent Solyc04g064880 gene can improve the resistance of the plant to the ToBRFV, and a technical support is provided for the breeding of a ToBRFV resistant variety of the plant.

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The embodiment of the invention provides nano-enzyme hydrogel for promoting scar-free wound repair through inflammation regulation and c-Jun gene silencing and a preparation method of the nano-enzyme hydrogel. The nano-enzyme hydrogel is prepared from the following components in parts by mass: synthesized epsilon-polylysine modified CeO2 nano-enzyme particles and aldehyde hyaluronic acid, an instant Schiff base reaction between epsilon PL-CeO2 surface amino groups and HA-CHO molecule aldehyde groups is utilized, in-situ mixing is performed to form a hydrogel network, in addition, bFGF and siRNA are loaded into PLGA microspheres, and the hydrogel network is formed through in-situ mixing. And further embedding into the hydrogel to obtain the nano-enzyme hydrogel. The obtained nano-enzyme hydrogel has the functions of inflammation regulation, cell proliferation promotion, vascular regeneration promotion, c-Jun gene silencing and the like, and scar-free healing of skin wounds is remarkably promoted.

The invention discloses application of a Chibby1 (CBY1) inhibitor in preparation of an anti-osteoporosis medicine for promoting bone formation, and belongs to the technical field of biological medicines. It is found for the first time that CBY1 can inhibit bone marrow stromal cells from differentiating into osteoblasts, and bone marrow stromal cells with CBY1 gene silencing are increased in osteoblast differentiation. According to the invention, it is proposed for the first time that osteoblast differentiation can be promoted by reducing CBY1 expression or inhibiting the CBY1 function, and the CBY1 inhibitor has the potential of increasing osteogenic activity and preventing and treating osteoporosis by inhibiting CBY1 expression or inhibiting the CBY1 function.

The invention relates to the technical field of plant genetic engineering, and provides a receptor-like kinase gene for improving Orobanche coerulescens parasitism resistance of sunflower and application of the receptor-like kinase gene. The invention discovers an amino acid sequence of the sunflower receptor kinase and a nucleotide sequence of the coding gene HaCERK1, and provides a target sequence for silencing the HaCERK1 gene, a virus-mediated gene silencing system and a silencing method based on the amino acid sequence, so that the expression level of the sunflower receptor kinase HaCERK1 gene can be effectively inhibited, and the expression level of the sunflower receptor kinase HaCERK1 gene can be effectively inhibited. And a virus-mediated gene silencing system can be systematically diffused in sunflower and sunflower orobanche tissues, so that the expression of the HaCERK1 gene is effectively interfered. According to the virus-mediated gene silencing system and method provided by the invention, the sunflower gene function research efficiency is remarkably improved, and the time and the cost are greatly reduced.

The invention belongs to the technical field of agricultural biology, and particularly discloses an RcPR5 gene for regulating and controlling gray mold resistance of Chinese roses and application of the RcPR5 gene, and the nucleotide sequence of the RcPR5 gene is shown as SEQ ID NO.1. The RcPR5 gene provided by the invention is remarkably associated with the gray mold resistance, plays an important role in plant immunity, regulates and controls immunity through an RNA processing complex, and has the advantages that the resistance of Chinese roses to gray mold is regulated and controlled; transcriptome difference analysis shows that the expression quantity of the RcPR5 is induced by grey mould to be remarkably up-regulated, after the RcPR5 gene is silenced, the petal scab is remarkably increased, it is proved that the RcPR5 gene really plays a role in the disease resistance process of the gray mold of the Chinese rose, breeding of the gray mold resistant Chinese rose can be conducted through the gene by adopting the modern gene engineering technology, and the breeding cost is lowered. And new germplasm is developed and introduced.

The invention belongs to the technical field of gene cotton resistance breeding, and particularly relates to application of cotton GhGSTF8 and GbGSTF8 genes in cotton verticillium wilt resistance. According to the application, the expression level of the GhGSTF8 gene in leaves and receptacles of cotton is relatively high through qRT-PCR (Quantitative Reverse Transcription-Polymerase Chain Reaction) detection; the expression level of the GbGSTF8 gene in leaves and stems of cotton is high. VIGS experiments show that after the GhGSTF8 gene is silenced, a new plant body shows an obvious susceptible character characteristic to verticillium wilt infection. After the GbGSTF8 gene is silenced, the silenced plant shows the character characteristic of resistance. Based on the research result, a certain application foundation can be laid for the molecular mechanism research of verticillium wilt resistance and the cultivation of new varieties of novel verticillium wilt-resistant plants.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses a Pennisetum purpureum PpPDS gene VIGS silencing system as well as a construction method and application thereof. The invention provides an application of the VIGS silencing system in at least one of the following applications: A1, silencing a target gene in pennisetum purpureum; a2, preparing target gene silent pennisetum purpureum; a3, verifying the function of the target gene; and A4, creating the germplasm of the pennisetum According to the invention, the VIGS silencing system of the pennisetum purpureum PpPDS gene is successfully constructed for the first time, and the pennisetum purpureum PpPDS gene is successfully silenced. By adopting the VIGS silencing system disclosed by the invention, the expression level of the PpPDS gene of the pennisetum purpureum can be effectively reduced, and whitening phenotype appears on leaves, so that the VIGS silencing system of the PpPDS gene of the pennisetum purpureum is successfully established.

The present disclosure provides in one aspect a method of enhancing an immune response in a subject in need thereof. In certain embodiments, the method comprises administering to the subject an effective amount of a gene silencing system comprising a plurality of gRNAs, wherein the gene silencing system decreases expression of at least one endogenous immunosuppressive gene in a target cell, thereby enhancing the immune response. Also included are methods and compositions for enhancing anti-tumor immune responses in subject in need thereof.

The invention relates to a key gene CbLTP63 for regulating and controlling salt stress response of catalpa bungei and application of the key gene CbLTP63, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the CbLTP63 gene is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a catalpa bungei salt stress response key gene CbLTP63. Identifying that the CbLTP63-GFP subcell is positioned on a cell membrane through an agrobacterium injection method; through qRT-PCR analysis and identification, the gene responds to salt stress on the transcriptional level and is down-regulated by H2O2, ABA and SA; through transgenosis identification, the gene is a catalpa bungei salt stress response negative regulation gene, and the salt tolerance of catalpa bungei calluses after gene silencing is obviously enhanced; the invention also relates to utilization of the gene in regulating stress response of catalpa bungei. The invention discloses a catalpa bungei salt stress response key gene CbLTP63, which has important application value in the fields of catalpa bungei gene engineering and clonal forestry.

The invention discloses application of a Cslnc924 gene in diagnosis or prevention and treatment of tea tree anthracnose, and relates to the technical field of prevention and treatment of tea tree anthracnose. The Cslnc924 gene is used for promoting the expression of the Cslnc924 gene so as to enhance the resistance of tea trees to colletotrichum gloeosporioides, and the cDNA full-length sequence of the Cslnc924 gene is as shown in SEQ ID No.1. The method has the beneficial effects that on the basis of an agrobacterium tumefaciens-mediated transient overexpression technology and an antisense oligonucleotide-mediated gene silencing technology, the Cslnc924 gene is overexpressed and silenced on tea tree leaves respectively, and an anthrax inoculation test is carried out. Results show that when Cslnc924 is overexpressed, compared with a control group, CsmiR390 is remarkably up-regulated, downstream CsARFs (CsARF2.1, CsARF2.2, CsARF3 and CsARF4.1) of CsmiR390 are remarkably down-regulated, at the moment, resistance of tea trees to colletotrichum gloeosporioides is enhanced, and the area of disease spots is remarkably reduced.

The invention belongs to the technical field of plant biology, and particularly relates to an establishment method and application of a TRV virus induced canna endogenous gene silencing expression system. The method comprises the following steps: constructing a recombinant pTRV2-PDS vector through a CiPDS specific fragment, preparing virus juice by using an agrobacterium-mediated virus infection system and taking nicotiana benthamiana as a transition host, and realizing effective infection of the TRV virus on canna through friction inoculation. According to the method, a VIGS technical system is established in canna for the first time, the limitation of a traditional heterologous transformation technology is broken through, and the problem that research on canna flower organ development is lagged due to lack of an efficient gene function verification tool is solved. Meanwhile, the silence stability and phenotype observability are remarkably improved by optimizing the temperature and selecting inoculation materials.