659 results about "Gene silencing" patented technology

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses an application of a GHERF114 gene in regulating and controlling salt stress tolerance and drought stress tolerance of a plant. The ID (Identity) of the gene is GHA05G2445. According to the invention, an arabidopsis thaliana homozygous line over-expressing the GHERF114 gene is obtained through a transgenic technology; through phenotypic change after salt stress treatment and drought stress treatment, leaf wilting degree, rosette leaf size and form comparison, in-vitro leaf water loss rate, leaf relative water content, chlorophyll content and the like verification, it is indicated that the tolerance of transgenic arabidopsis thaliana to salt stress and drought stress is remarkably reduced, and a TRV: GHERF114 gene silent plant is obtained through a VIGS technology; compared with a control group after salt stress and drought stress treatment, the salt tolerance and the drought resistance of silent plants are remarkably improved. Results show that the GHERF114 plays an important role in plant salt stress and drought stress response processes, has important significance in breeding and research for improving salt tolerance and drought tolerance of cotton, and can be applied to screening of stress-resistant cotton varieties through a gene knockout technology.

The invention discloses an upland cotton wild germline salt-tolerant gene GhSHOU4 and application thereof, the gene ID of the cotton gene GhSHOU4 is GHA05G1741, and the nucleotide sequence of the cotton gene GhSHOU4 is as shown in the sequence 1. According to the invention, the wild germline gene GhSHOU4 of the upland cotton is cloned from the wild germline of the upland cotton, and a subcellular localization result shows that the GhSHOU4 protein is localized on a cytoplasmic membrane. Gene silencing cotton is obtained by using a VIGS technology, phenotype identification and physiological and biochemical index determination after salt stress treatment show that the malondialdehyde content of a gene silencing plant is significantly increased, and it is proved that the tolerance of the cotton after silencing the gene under salt stress is significantly reduced. Good gene resources are provided for salt-tolerant molecular breeding and genetic improvement of cotton.

The invention discloses a method for improving citrus yellow vein disease resistance by using a ClPsaN gene. The method comprises the following steps: (1) cloning a VIGS fragment of the citrus ClPsaN gene; (2) constructing two VIGS expression vectors of TRV and CLBV; and (3) transforming the citrus by the VIGS expression vector to obtain a citrus ClPsaN gene silenced VIGS plant. According to the invention, the VIGS vector of the Citrus ClPsaN gene is constructed, and then the Citrus is transformed, so that the obtained Citrus plant can show obvious resistance to the Citrus yellow vein clearing virus, the titer of the Citrus yellow vein clearing virus can be reduced by 22-34%, the accumulation amount of the virus protein is reduced by 35-50%, and the attack degree of the Citrus yellow vein clearing virus is obviously reduced; the TRV silencing of the citrus ClPsaN gene does not influence the phenotype of a citrus plant, and the citrus ClPsaN gene can be used as a candidate gene for citrus yellow vein disease resistance breeding together with a plurality of citrus yellow vein disease-resistant and susceptible genes by utilizing the cooperation of VIGS silencing, RNA interference, gene editing and other technologies.

The invention discloses an application of RhALEU protein and a coding gene thereof in senescence regulation and control of Chinese rose petals. According to the invention, the RhALEU gene is screened from a Chinese rose transcriptome database, and research finds that the expression of RhALEU is induced in the whole petal development stage. A VIGS technology is utilized to verify that the RhALEU gene has a function of promoting the aging of Chinese rose petals. The silence of the RhALEU gene slows down the speed of petals from level 5 to loss of ornamental value. In petals with the silent RhALEU gene, the expression of the senescence-related gene SAG12 is remarkably reduced. Research results of the invention reveal the effect of the RhALEU gene in natural aging of petals, a new thought and technical means are provided for regulating and controlling plant flower aging, especially post-harvest preservation of fresh Chinese rose flowers, a new flower regulation and control research field is developed, and the RhALEU gene has great academic value and important production and application prospects.

The present invention provides methods, computer systems and computer-implemented products for making synthetic regulatory elements, and provides polynucleotide's, transgenic cells, and transgenic organisms (including viruses and viral vectors) produced by these methods. The invention thereby provides regulatory sequences to meet various gene expression objectives, including the ability to stack a plurality of heterologous genes for expression in a single cell, while avoiding gene silencing or reduced expression levels.

The invention discloses application of an Afa04Dg76000 gene in resistance of an oat herbicide. The Afa04Dg76000 gene is knocked down to reduce the resistance of the oat herbicide, and the Afa04Dg76000 gene is overexpressed to improve the resistance of the oat herbicide; the nucleotide sequence of the Afa04Dg76000 gene is as shown in SEQ ID NO. 1, and the amino acid sequence of the Afa04Dg76000 gene is as shown in SEQ ID NO. 2. According to the application of the Afa04Dg76000 gene in the herbicide resistance of the oats, a gene knock-down and overexpression strain of the Afa04Dg76000 is constructed through a TRV virus-mediated gene silencing (VIGS) system and a genetic transformation system of the oats, the herbicide resistance function of the Afa04Dg76000 is proved, and a new target is provided for germplasm improvement of cultivated oats.

The invention provides a protein for synthesizing an octadecene compound and a method for synthesizing the octadecene compound. Relates to the field of enzymology. The amino acid sequence of the protein has more than 95% of consistency with the sequence as shown in SEQ ID No.3. The protein, hexadecanoic acid specific thioesterase, Z11 desaturase, gene silencing suppressor and vegetable fat synthesis related transcription factors are jointly expressed in plants and can be used for synthesizing (Z)-13-octadecenoic acid.

The invention provides an application of a PavERF110 gene in regulating and controlling the ripening direction of sweet cherry fruits, and belongs to the technical field of molecular breeding. The nucleotide sequence of the PavERF110 gene is as shown in SEQ ID NO.1, and the amino acid sequence of the encoded protein of the PavERF110 gene is as shown in SEQ ID NO.2. The invention also provides an overexpression vector and a VIGS gene silencing vector constructed by the gene, a construction method of the overexpression vector and the VIGS gene silencing vector, and a recombinant microorganism containing the vectors. Engineering bacteria containing related carriers are injected and infected at the equator of the fruits 25 days after the sweet cherries blossom, and it is found that overexpression of the PavERF110 gene can accelerate fruit coloring and increase the expression level of the mature related genes. New technical support and theoretical basis are provided for sweet cherry fruit quality improvement and ripening regulation, market requirements can be met, and meanwhile understanding of a fruit ripening transcription regulation mechanism is deepened.

The invention discloses an RcWRKY29 gene and application of the RcWRKY29 gene in enhancing the resistance of Chinese roses to gray mold. The nucleotide sequence of the RcWRKY29 gene is shown as SEQ ID NO: 1. Researches find that the gene is specifically and highly expressed in Chinese rose petals, and the expression quantity is remarkably increased within 24-48 hours after botrytis cinerea infection. After RcWRKY29 expression is instantaneously inhibited through a virus-induced gene silencing technology, the scab area of Chinese rose petals is increased, and the disease resistance is remarkably reduced; otherwise, overexpression of the gene can significantly enhance disease resistance and reduce scab area and cell damage. The invention provides a recombinant vector containing RcWRKY29, an engineering bacterium and a detection kit, and develops a method for enhancing the disease resistance of Chinese rose based on an agrobacterium-mediated transformation technology. The invention provides a new target for Chinese rose gray mold resistance molecular breeding, can effectively reduce postharvest disease loss, and has important economic value and popularization and application prospects.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms, dsRNA and application thereof. Specifically, the nucleotide sequences of the three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms are respectively SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The recombinant Hc beta GRP protein is successfully obtained through a prokaryotic expression system, an in-vitro bacteriostatic test proves that the recombinant Hc beta GRP protein, the Hc beta GRP protein and the dsRNA have direct bacteriostatic activity, dsRNA is designed based on a pattern recognition protein Hc beta GRP gene, a hyphantria cunea Hc beta GRP dsRNA interference system implemented by using an injection method is established, an obvious gene silencing effect is obtained by injecting the Hc beta GRP dsRNA interference system into hyphantria cunea, and the Hc beta GRP dsRNA interference system is applied to the hyphantria cunea. The technical support is provided for the application of the Hc beta GRP RNAi technology. Besides, the dsRNA of the hyphantria cunea Hc beta GRP is combined with the biocontrol bacteria by utilizing an established hyphantria cunea Hc beta GRP RNA interference system, so that the control effect of the biocontrol bacteria can be remarkably improved.

The invention discloses a FaABI3 gene and application thereof in regulation and control of strawberry fruit maturity and strawberry flavonoid content, and relates to the field of gene engineering, the nucleotide sequence of the FaABI3 gene is shown as SEQ ID NO.1, and the amino acid sequence of protein encoded by the FaABI3 gene is shown as SEQ ID NO.2. A FaABI3 gene silencing vector is constructed and is used for infecting octaploid strawberry fruits in a small green period, so that the strawberry fruit maturity and strawberry flavonoid content can be regulated and controlled, and the strawberry fruit maturity and strawberry flavonoid content can be regulated and controlled. Strawberry fruits with the FaABI3 gene silenced are obtained through screening according to the gene expression quantity in the fruits, it is indicated that by changing the expression quantity of the FaABI3 gene in strawberries, the growth and development of the strawberries can be regulated and controlled, the FaABI3 gene can be applied to strawberry genetic breeding, an important foundation is laid for strawberry variety improvement, and the method has a wide application prospect. The application of the FaABI3 gene in increasing the content of strawberry flavonoid spinosin, sophoricoside, formononin and delphinidin is found for the first time, and further understanding of a strawberry flavonoid regulation mechanism is facilitated, so that the strawberry flavonoid content can be regulated by utilizing a biotechnological means, and an important foundation is laid for strawberry variety improvement.

The invention discloses an RGD-click chemical crosslinking siRNA nano-carrier, a preparation method thereof and application of the nano-carrier in preparation of a medicine for treating secondary thyroidism, and belongs to the technical field of medicines.The nano-carrier is RGD-PEG-PLys (N), and the preparation method of the nano-carrier comprises the steps of synthesis of PLys (ss-DBCO), synthesis of RGD-PEG-PLys (N) and the like. According to the application, the nano-carrier is used for preparing siRNA composite nanoparticles; vascular endothelial targeting (RGD), dynamic stability (click crosslinking) and microenvironment responsiveness (disulfide bond) are organically combined for the first time to achieve mutual synergistic interaction, and the effect ceiling of an existing material is broken through; according to the siRNA composite nanoparticle, the silence effect of the PTH gene as long as 70 days can be achieved through single intravenous injection, the PTH inhibition rate is larger than 85%, the siRNA accumulation amount in parathyroid gland tissue is remarkably increased through RGD targeting (8.6 times that of a non-targeting group), the liver and kidney distribution amount is reduced by 60%, and the system toxicity is controllable.

The invention belongs to the technical field of cytotoxicity intervention, and particularly relates to application of an iNOS inhibitor in preparation of a medicine for relieving cellular injury caused by toxin combined exposure. KK-1 cells serve as a research model, it is shown for the first time that MC-LR and NaNOS combined contamination can remarkably up-regulate the expression level of iNOS protein, and it is found through tests that Nos2 gene silencing can remarkably relieve tight junction damage and cell apoptosis caused by toxin combined exposure in the KK-1 cells; it is revealed for the first time that iNOS is a core target of toxin combined exposure induced cell tight junction damage and apoptosis, and the blank of toxic mechanism research in toxin combined exposure is filled. The application provides a new direction for a detoxification strategy of environmental toxin combined exposure, provides a theoretical basis for developing an iNOS-targeted inhibitor, and also provides a new target for etiological recognition, early diagnosis and prevention of related diseases caused by environmental toxin combined exposure.

The invention relates to the technical field of biology, in particular to a vitis davidii VdYUCCA10 gene as well as an encoding protein and application thereof. The vitis davidii VdYUCCA10 gene is located on a chromosome 7 of vitis davidii and is distributed in a 2271185-2274940 region, and the nucleotide sequence of the vitis davidii VdYUCCA10 gene is shown as SEQ ID NO. 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID No.2. The invention also discloses an application of the vitis davidii VdYUCCA10 gene in enhancing the resistance of grape fruits to pathogenic bacteria after silencing. After the VdYUCCA10 gene provided by the invention is silenced, the resistance of grapes to anthracnose can be improved, and a theoretical basis is provided for grape anthracnose resistance breeding.

The invention provides an hpRNA interference vector for laver and application of the hpRNA interference vector, and belongs to the technical field of genetic engineering and algae biology. According to the hpRNA interference vector, a binary vector pBI121 is used as a skeleton vector, a laver endogenous Actin promoter and a multiple cloning site segment are inserted, the multiple cloning sites allow target gene segments to be cloned in a forward and reverse repetition mode, the target gene segments are spaced by introns, and therefore an efficient hpRNA structure is formed after transcription, and the efficient hpRNA interference vector is obtained. The gene can be directly used for silencing important genes related to growth and development, metabolic regulation, stress response and the like in the laver. The invention further provides a construction method of the hpRNA interference vector and application of the hpRNA interference vector in silencing laver genes and provides a method for silencing laver gene expression, the gene silencing effect is remarkable, experimental results can be repeated, and a powerful tool is provided for molecular breeding and genetic improvement of laver.

The invention discloses a gene RhNADP-LIKE for regulating and controlling Chinese rose flower aging, and particularly relates to the technical field of molecular biology and horticultural plant physiology. According to the invention, bottle insertion life phenotype data of 358 parts of core germplasm of tetraploid Chinese rose is screened through genome-wide association analysis (GWAS), and the gene RhNADP-LIKE significantly associated with senescence is identified. The gene accelerates the aging process of petals by regulating the accumulation of reactive oxygen species (ROS). After RhNADP-LIKE expression is inhibited by using a virus-induced gene silencing (VIGS) technology, the blooming period of rosa chinensis flowers is prolonged by about 2 days, and the ROS level is remarkably reduced. The invention provides a molecular target for prolonging the vase life of the Chinese rose, can be applied to gene editing breeding or development of an environment-friendly preservative, reduces the dependence of chemical agents, and improves the economic benefit and sustainable development potential of the cut flower industry.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention provides application of a TaNAC42 gene in regulation and control of wheat stem rust resistance, and belongs to the technical field of gene functions. It is found for the first time that the TaNAC42 gene of wheat is related to the wheat stem rust resistance, the TaNAC42 gene is induced to be silenced through viruses, the wheat stem rust resistance can be enhanced, wheat plants are easier to resist diseases, the TaNAC42 gene is over-expressed through the agrobacterium tumefaciens-mediated transient over-expression technology, the wheat stem rust resistance can be weakened, the wheat plants are more susceptible to diseases, and the wheat plants are more susceptible to diseases. Namely, the TaNAC42 gene can negatively participate in regulating and controlling the resistance of the wheat to the Puccinia graminicola, a new target gene is provided for improved breeding of wheat Puccinia graminicola resistance molecules, and the TaNAC42 gene has important utilization value in wheat production and breeding.

The invention discloses a VIGS silencing system of walnut JrPDS gene as well as a construction method and application of the VIGS silencing system, and relates to the technical field of biology. The walnut JrPDS gene interference fragment as shown in SEQ ID NO.1 provided by the invention can effectively interfere the expression of the walnut endogenous JrPDS gene, so that the walnut seedling endogenous JrPDS gene is silenced, the silencing rate can reach 48%, and the walnut JrPDS gene interference fragment is used for identifying the function of the walnut endogenous gene.

The application discloses a kind of carrier, kit and application for long-acting gene knockdown of animal parasitic nematode, belong to the field of animal parasitic disease prevention and control.The lentivirus vector in the application integrates specific coding sequence to the genome of the infected nematode, stably and continuously expresses short hairpin RNA (shRNA) in the nematode, efficiently produces primary single-stranded small RNA (siRNA), these siRNAs target homologous target gene mRNA to cause its degradation, realize stable and continuous gene silencing effect.The application breaks through the bottleneck of traditional animal parasitic nematode RNA interference (RNAi) technology with low efficiency and instability, significantly improves the timeliness of conventional RNAi, can be used to establish long-acting RNAi technology system of animal parasitic nematode, has application value in the research of host in vivo process such as nematode infection, parasitism and pathogenicity and animal parasitic nematode disease prevention and control.

The invention discloses an application of a tomato Solyc04g064880 gene in regulation and control of ToBRFV resistance of a plant, a Solyc04g064880 gene silencing vector pTRV2 is constructed: S14880, agrobacterium tumefaciens is transformed, positive bacteria obtained by screening are inoculated into a target plant to obtain a Solyc04g064880 gene silencing strain, and compared with a contrast, the Solyc04g064880 gene silencing strain has the advantages that the ToBRFV accumulation amount in the plant is reduced, and the ToBRFV resistance of the plant can be regulated and controlled. Therefore, the silent Solyc04g064880 gene can improve the resistance of the plant to the ToBRFV, and a technical support is provided for the breeding of a ToBRFV resistant variety of the plant.

The present disclosure relates to recombinant transcriptional repressor polypeptides that are capable of being targeted to specific loci, as well as methods of using these recombinant polypeptides for reducing expression of a target nucleic acid in plants.

The invention belongs to the field of tobacco gene engineering, and particularly relates to application of a ferulic acid synthetic gene NtKAT2.2 in tobacco. The nucleotide sequence of the tobacco ferulic acid synthetic gene NtKAT2.2 is as shown in SEQ ID No. 1 (sequence identifier number 1). The expression quantity of the NtKAT2.2 gene is in negative correlation with the content of ferulic acid in tobacco. Preliminary research results show that the NtKAT2.2 gene is highly related to the content of the ferulic acid in tobacco, and after the gene is silenced, the content of the ferulic acid in a tobacco strain after gene silencing can be obviously increased. Based on the result, the inventor believes that on the basis of further deep research on the gene, a good technical foundation is expected to be laid for tobacco quality improvement and further new variety cultivation.

The invention discloses a brown planthopper gene NlCYP303A1 and a nano nucleic acid pesticide of the brown planthopper gene NlCYP303A1. The nucleotide sequence of the gene NlCYP303A1 disclosed by the invention is as shown in SEQ ID NO: 1; the nano nucleic acid pesticide is mainly prepared from zinc nitrate hexahydrate, dsN1CYP303A1 and 2-methylimidazole. The gene NlCYP303A1 closely related to the ecdysis process of the brown planthopper is identified, and silencing of the gene can cause ecdysis failure of the brown planthopper and reduce the survival rate of the brown planthopper; furthermore, a dsN1CYP303A1-coated ZIF-8 nano nucleic acid pesticide delivery system is constructed on the basis of a nano carrier ZIF-8, and the biological activity of the dsN1CYP303A1-coated ZIF-8 nano nucleic acid pesticide delivery system is measured; results show that the nano nucleic acid pesticide delivery system overcomes the defect that the service life of dsRNA is short, and the control effect of the nano nucleic acid pesticide delivery system on brown planthopper is remarkably improved. The potential application value of RNAi in pest control is improved, and a new strategy is provided for pest drug resistance treatment.

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention belongs to the technical field of plant breeding, and particularly relates to application of a GhPSKR1 gene in regulation and control of plant salt tolerance. The nucleotide sequence of the GhPSKR1 gene provided by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of the protein expressed by the GhPSKR1 gene is as shown in SEQ ID NO. 2. A research result proves that the growth inhibition of a GhPSKR1 gene silent plant under salt stress treatment is more obvious, the salt damage characteristic is more obvious, sodium ion accumulation in leaves and roots is increased, and the GhPSKR1 gene silent plant is more sensitive to salt stress; under salt stress treatment, the root length of a GhPSKR1 gene overexpression transgenic homozygous plant is remarkably elongated compared with that of wild arabidopsis thaliana, and the GhPSKR1 gene has good stress resistance, so that the GhPSKR1 gene plays a positive regulation role in regulating plant salt stress.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.