367 results about "Gene silencing" patented technology

The invention relates to the technical field of biology, in particular to a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention discloses a verticillium dahliae virulence gene VdPHO23like. The verticillium dahliae virulence gene VdPHO23like comprises a polynucleotide sequence for coding an amino acid sequence of SEQ ID NO: 3. According to the invention, expression of the gene is inhibited in a targeted manner through an RNA interference mediated gene silencing technology so as to weaken virulence of pathogenic bacteria, and an efficient, specific and environment-friendly comprehensive prevention and control strategy is provided for verticillium wilt of crops such as cotton.

The invention discloses three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms, dsRNA and application thereof. Specifically, the nucleotide sequences of the three pattern recognition protein Hc beta GRP genes for preventing and treating fall webworms are respectively SEQ ID NO: 1, SEQ ID NO: 2 and SEQ ID NO: 3. The recombinant Hc beta GRP protein is successfully obtained through a prokaryotic expression system, an in-vitro bacteriostatic test proves that the recombinant Hc beta GRP protein, the Hc beta GRP protein and the dsRNA have direct bacteriostatic activity, dsRNA is designed based on a pattern recognition protein Hc beta GRP gene, a hyphantria cunea Hc beta GRP dsRNA interference system implemented by using an injection method is established, an obvious gene silencing effect is obtained by injecting the Hc beta GRP dsRNA interference system into hyphantria cunea, and the Hc beta GRP dsRNA interference system is applied to the hyphantria cunea. The technical support is provided for the application of the Hc beta GRP RNAi technology. Besides, the dsRNA of the hyphantria cunea Hc beta GRP is combined with the biocontrol bacteria by utilizing an established hyphantria cunea Hc beta GRP RNA interference system, so that the control effect of the biocontrol bacteria can be remarkably improved.

The invention discloses an RGD-click chemical crosslinking siRNA nano-carrier, a preparation method thereof and application of the nano-carrier in preparation of a medicine for treating secondary thyroidism, and belongs to the technical field of medicines.The nano-carrier is RGD-PEG-PLys (N), and the preparation method of the nano-carrier comprises the steps of synthesis of PLys (ss-DBCO), synthesis of RGD-PEG-PLys (N) and the like. According to the application, the nano-carrier is used for preparing siRNA composite nanoparticles; vascular endothelial targeting (RGD), dynamic stability (click crosslinking) and microenvironment responsiveness (disulfide bond) are organically combined for the first time to achieve mutual synergistic interaction, and the effect ceiling of an existing material is broken through; according to the siRNA composite nanoparticle, the silence effect of the PTH gene as long as 70 days can be achieved through single intravenous injection, the PTH inhibition rate is larger than 85%, the siRNA accumulation amount in parathyroid gland tissue is remarkably increased through RGD targeting (8.6 times that of a non-targeting group), the liver and kidney distribution amount is reduced by 60%, and the system toxicity is controllable.

The invention belongs to the technical field of cytotoxicity intervention, and particularly relates to application of an iNOS inhibitor in preparation of a medicine for relieving cellular injury caused by toxin combined exposure. KK-1 cells serve as a research model, it is shown for the first time that MC-LR and NaNOS combined contamination can remarkably up-regulate the expression level of iNOS protein, and it is found through tests that Nos2 gene silencing can remarkably relieve tight junction damage and cell apoptosis caused by toxin combined exposure in the KK-1 cells; it is revealed for the first time that iNOS is a core target of toxin combined exposure induced cell tight junction damage and apoptosis, and the blank of toxic mechanism research in toxin combined exposure is filled. The application provides a new direction for a detoxification strategy of environmental toxin combined exposure, provides a theoretical basis for developing an iNOS-targeted inhibitor, and also provides a new target for etiological recognition, early diagnosis and prevention of related diseases caused by environmental toxin combined exposure.

The invention relates to the technical field of biology, in particular to a vitis davidii VdYUCCA10 gene as well as an encoding protein and application thereof. The vitis davidii VdYUCCA10 gene is located on a chromosome 7 of vitis davidii and is distributed in a 2271185-2274940 region, and the nucleotide sequence of the vitis davidii VdYUCCA10 gene is shown as SEQ ID NO. 1. The amino acid sequence of the protein coded by the gene is as shown in SEQ ID No.2. The invention also discloses an application of the vitis davidii VdYUCCA10 gene in enhancing the resistance of grape fruits to pathogenic bacteria after silencing. After the VdYUCCA10 gene provided by the invention is silenced, the resistance of grapes to anthracnose can be improved, and a theoretical basis is provided for grape anthracnose resistance breeding.

The invention provides an hpRNA interference vector for laver and application of the hpRNA interference vector, and belongs to the technical field of genetic engineering and algae biology. According to the hpRNA interference vector, a binary vector pBI121 is used as a skeleton vector, a laver endogenous Actin promoter and a multiple cloning site segment are inserted, the multiple cloning sites allow target gene segments to be cloned in a forward and reverse repetition mode, the target gene segments are spaced by introns, and therefore an efficient hpRNA structure is formed after transcription, and the efficient hpRNA interference vector is obtained. The gene can be directly used for silencing important genes related to growth and development, metabolic regulation, stress response and the like in the laver. The invention further provides a construction method of the hpRNA interference vector and application of the hpRNA interference vector in silencing laver genes and provides a method for silencing laver gene expression, the gene silencing effect is remarkable, experimental results can be repeated, and a powerful tool is provided for molecular breeding and genetic improvement of laver.

The invention discloses a gene RhNADP-LIKE for regulating and controlling Chinese rose flower aging, and particularly relates to the technical field of molecular biology and horticultural plant physiology. According to the invention, bottle insertion life phenotype data of 358 parts of core germplasm of tetraploid Chinese rose is screened through genome-wide association analysis (GWAS), and the gene RhNADP-LIKE significantly associated with senescence is identified. The gene accelerates the aging process of petals by regulating the accumulation of reactive oxygen species (ROS). After RhNADP-LIKE expression is inhibited by using a virus-induced gene silencing (VIGS) technology, the blooming period of rosa chinensis flowers is prolonged by about 2 days, and the ROS level is remarkably reduced. The invention provides a molecular target for prolonging the vase life of the Chinese rose, can be applied to gene editing breeding or development of an environment-friendly preservative, reduces the dependence of chemical agents, and improves the economic benefit and sustainable development potential of the cut flower industry.

The invention discloses an upland cotton GhCRK29 gene as well as an encoding protein and application thereof, the GhCRK29 gene is separated from cotton, the nucleotide sequence of the GhCRK29 gene is as shown in SEQ ID No.1, and the amino acid sequence of the encoding protein of the GhCRK29 gene is as shown in SEQ ID No.2. According to the invention, a virus-induced gene silencing technology and a transgenic overexpression technology are further utilized to carry out preliminary research on the function of the cotton GhCRK29 gene in cotton verticillium wilt resistance, and a result shows that the verticillium wilt resistance of a cotton seedling plant is obviously reduced after the GhCRK29 gene in cotton is silenced; after the GhCRK29 gene is over-expressed in arabidopsis thaliana or cotton, the verticillium wilt resistance of arabidopsis thaliana or cotton is remarkably improved, and the resistance of a GhCRK29 gene forward regulation plant to verticillium dahliae is determined; the invention has application prospects in the aspects of improving the verticillium wilt resistance of plants or cultivating verticillium wilt resistant plant varieties and the like.

The invention provides application of a TaNAC42 gene in regulation and control of wheat stem rust resistance, and belongs to the technical field of gene functions. It is found for the first time that the TaNAC42 gene of wheat is related to the wheat stem rust resistance, the TaNAC42 gene is induced to be silenced through viruses, the wheat stem rust resistance can be enhanced, wheat plants are easier to resist diseases, the TaNAC42 gene is over-expressed through the agrobacterium tumefaciens-mediated transient over-expression technology, the wheat stem rust resistance can be weakened, the wheat plants are more susceptible to diseases, and the wheat plants are more susceptible to diseases. Namely, the TaNAC42 gene can negatively participate in regulating and controlling the resistance of the wheat to the Puccinia graminicola, a new target gene is provided for improved breeding of wheat Puccinia graminicola resistance molecules, and the TaNAC42 gene has important utilization value in wheat production and breeding.

The application discloses a kind of carrier, kit and application for long-acting gene knockdown of animal parasitic nematode, belong to the field of animal parasitic disease prevention and control.The lentivirus vector in the application integrates specific coding sequence to the genome of the infected nematode, stably and continuously expresses short hairpin RNA (shRNA) in the nematode, efficiently produces primary single-stranded small RNA (siRNA), these siRNAs target homologous target gene mRNA to cause its degradation, realize stable and continuous gene silencing effect.The application breaks through the bottleneck of traditional animal parasitic nematode RNA interference (RNAi) technology with low efficiency and instability, significantly improves the timeliness of conventional RNAi, can be used to establish long-acting RNAi technology system of animal parasitic nematode, has application value in the research of host in vivo process such as nematode infection, parasitism and pathogenicity and animal parasitic nematode disease prevention and control.

The invention relates to a wheat stem rot regulatory gene Tatrx-m, and an encoding protein, a recombinant vector and an application of the wheat stem rot regulatory gene Tatrx-m. The genomic sequence of the gene is SEQ ID NO.1, the CDS sequence of the gene is SEQ ID NO.2, and thioredoxin shown as SEQ ID NO.3 is encoded. The invention further provides a specific primer pair used for gene identification, a silent vector (Tatrx-m-VIGS) and an overexpression vector (LGY-OE3-Tatrx-m) are constructed, and the function of the primer pair is verified through a virus-induced gene silencing (VIGS) technology and agrobacterium-mediated transformation of wheat. Experiments show that silence of Tatrx-m results in significant reduction of wheat stem rot resistance, and overexpression of the gene can improve resistance. Based on hypha quantification, DAB staining, H2O2 content and cell death analysis, Tatrx-m is revealed to enhance wheat resistance by regulating active oxygen removal and cell wall strengthening pathways. The gene TaTrx-m has the positive regulation effect in wheat stem rot for the first time, can be applied to disease-resistant molecular marker development, gene editing and transgenic breeding, provides core genetic resources for disease-resistant variety breeding, and has theoretical and application values.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses a double-stranded RNA (Ribonucleic Acid) aiming at hemiptera insects as well as a pesticide composition and application of the double-stranded RNA. The dsRNA provided by the invention is used as a biopesticide, has the characteristics of high efficiency, specificity, fast degradation and no residue, realizes the prevention and control of brown planthopper through an RNAi mechanism, and is beneficial to reducing the use of chemical pesticides. The invention provides a brand new brown planthopper target gene as a target of RNA interference. The survival rate result shows that the genes play an important role in the survival process of the brown planthopper, are effective targets for preventing and treating the brown planthopper by using a gene silencing means, and have a wide application prospect. Correspondingly, the invention provides a plurality of dsRNA sequences aiming at the targets, and part of the sequences show unprecedented brown planthopper control efficiency.

The invention discloses application of a Chibby1 (CBY1) inhibitor in preparation of an anti-osteoporosis medicine for promoting bone formation, and belongs to the technical field of biological medicines. It is found for the first time that CBY1 can inhibit bone marrow stromal cells from differentiating into osteoblasts, and bone marrow stromal cells with CBY1 gene silencing are increased in osteoblast differentiation. According to the invention, it is proposed for the first time that osteoblast differentiation can be promoted by reducing CBY1 expression or inhibiting the CBY1 function, and the CBY1 inhibitor has the potential of increasing osteogenic activity and preventing and treating osteoporosis by inhibiting CBY1 expression or inhibiting the CBY1 function.

The invention belongs to the technical field of agricultural biology, and particularly discloses an RcPR5 gene for regulating and controlling gray mold resistance of Chinese roses and application of the RcPR5 gene, and the nucleotide sequence of the RcPR5 gene is shown as SEQ ID NO.1. The RcPR5 gene provided by the invention is remarkably associated with the gray mold resistance, plays an important role in plant immunity, regulates and controls immunity through an RNA processing complex, and has the advantages that the resistance of Chinese roses to gray mold is regulated and controlled; transcriptome difference analysis shows that the expression quantity of the RcPR5 is induced by grey mould to be remarkably up-regulated, after the RcPR5 gene is silenced, the petal scab is remarkably increased, it is proved that the RcPR5 gene really plays a role in the disease resistance process of the gray mold of the Chinese rose, breeding of the gray mold resistant Chinese rose can be conducted through the gene by adopting the modern gene engineering technology, and the breeding cost is lowered. And new germplasm is developed and introduced.

The invention discloses a verticillium dahliae virulence gene, a verticillium dahliae virulence protein and application. The invention relates to the technical field of biology, and provides a method for preventing and treating cotton verticillium wilt based on RNAi (RNA interference) as well as related components and application thereof. Specifically, the invention discloses application of the verticillium wilt prevention and treatment by a method for inhibiting or silencing the expression of verticillium dahliae pathogenic gene VdHDAC (coding protein of the verticillium dahliae pathogenic gene VdHDAC is shown as SEQ ID NO: 3), the core of the verticillium wihliae pathogenic gene VdHDAC is to provide dsRNA molecules capable of targeting the gene, and the positive-sense strand of the dsRNA molecules is preferably selected from SEQ ID NO: 5, SEQ ID NO: 8, SEQ ID NO: 9 or SEQ ID NO: 11. Based on the molecule, the invention further provides a recombinant vector containing the coding sequence, a recombinant microorganism, a transgenic disease-resistant plant and an RNAi pesticide preparation. The scheme can be realized through host-induced gene silencing, microorganism-mediated gene silencing or preparation treatment and the like, the virulence of pathogenic bacteria can be effectively reduced, and a new technical approach is provided for green prevention and control of verticillium wilt.

The invention discloses a Pennisetum purpureum PpPDS gene VIGS silencing system as well as a construction method and application thereof. The invention provides an application of the VIGS silencing system in at least one of the following applications: A1, silencing a target gene in pennisetum purpureum; a2, preparing target gene silent pennisetum purpureum; a3, verifying the function of the target gene; and A4, creating the germplasm of the pennisetum According to the invention, the VIGS silencing system of the pennisetum purpureum PpPDS gene is successfully constructed for the first time, and the pennisetum purpureum PpPDS gene is successfully silenced. By adopting the VIGS silencing system disclosed by the invention, the expression level of the PpPDS gene of the pennisetum purpureum can be effectively reduced, and whitening phenotype appears on leaves, so that the VIGS silencing system of the PpPDS gene of the pennisetum purpureum is successfully established.

The invention relates to a key gene CbLTP63 for regulating and controlling salt stress response of catalpa bungei and application of the key gene CbLTP63, and belongs to the technical field of plant genetic engineering and biology. The nucleotide sequence of the CbLTP63 gene is as shown in Seq 1, and the coded amino acid sequence is as shown in Seq 2. The invention relates to cloning of a catalpa bungei salt stress response key gene CbLTP63. Identifying that the CbLTP63-GFP subcell is positioned on a cell membrane through an agrobacterium injection method; through qRT-PCR analysis and identification, the gene responds to salt stress on the transcriptional level and is down-regulated by H2O2, ABA and SA; through transgenosis identification, the gene is a catalpa bungei salt stress response negative regulation gene, and the salt tolerance of catalpa bungei calluses after gene silencing is obviously enhanced; the invention also relates to utilization of the gene in regulating stress response of catalpa bungei. The invention discloses a catalpa bungei salt stress response key gene CbLTP63, which has important application value in the fields of catalpa bungei gene engineering and clonal forestry.

The invention discloses application of a Cslnc924 gene in diagnosis or prevention and treatment of tea tree anthracnose, and relates to the technical field of prevention and treatment of tea tree anthracnose. The Cslnc924 gene is used for promoting the expression of the Cslnc924 gene so as to enhance the resistance of tea trees to colletotrichum gloeosporioides, and the cDNA full-length sequence of the Cslnc924 gene is as shown in SEQ ID No.1. The method has the beneficial effects that on the basis of an agrobacterium tumefaciens-mediated transient overexpression technology and an antisense oligonucleotide-mediated gene silencing technology, the Cslnc924 gene is overexpressed and silenced on tea tree leaves respectively, and an anthrax inoculation test is carried out. Results show that when Cslnc924 is overexpressed, compared with a control group, CsmiR390 is remarkably up-regulated, downstream CsARFs (CsARF2.1, CsARF2.2, CsARF3 and CsARF4.1) of CsmiR390 are remarkably down-regulated, at the moment, resistance of tea trees to colletotrichum gloeosporioides is enhanced, and the area of disease spots is remarkably reduced.

The invention belongs to the field of biological agriculture, and particularly relates to application of a wheat stripe rust effect protein gene HASP 38 in improvement of stripe rust resistance of wheat. The amino acid sequence of the wheat stripe rust effect protein HASP 38 provided by the invention is as shown in SEQ ID NO: 1, the nucleotide sequence of the wheat stripe rust effect protein gene HASP 38 is as shown in SEQ ID NO: 2, and an RNA interference vector of the gene is provided. According to the invention, an RNA (Ribonucleic Acid) interference vector of the gene is transformed into a Fielder wild type material, so that the HASP 38 gene silencing wheat with resistance to stripe rust CYR32 is obtained. According to the invention, the wheat stripe rust effect protein gene HASP 38 is determined to play a negative regulation role in wheat stripe rust resistance, and a stripe rust resistant wheat material is created by using the negative regulation factor, so that a new gene and germplasm resource are provided for wheat stripe rust resistant breeding.

The invention belongs to the technical field of biology, and particularly relates to application of a GmNIC1 gene and a GmNIC2 gene to regulation and control of soybean cyst nematode resistance of plants, and the nucleotide sequence of the GmNIC1 gene is shown as SEQ ID NO.1; the nucleotide sequence of the GmNIC2 gene is as shown in SEQ ID NO. 2. The invention discloses the negative regulation effect of GmNIC1 and GmNIC2 genes in the resistance of soybean to cyst nematode (SCN) for the first time, and by constructing gene silencing mutants of GmNIC1 and GmNIC2, it is verified that the resistance of soybean to SCN can be remarkably enhanced due to function deficiency of the gene family. The application provides a new target for deep understanding of a soybean-nematode interaction mechanism, and provides a brand new gene resource and technical path for molecular design breeding of resistant soybeans.

The invention discloses a stropharia rugoso-annulata SRU6 gene promoter, a silent vector containing the promoter and application of silent stropharia rugoso-annulata endogenous genes, and relates to the field of molecular biology and gene engineering. According to the principle of shRNA mediated gene silencing, a silencing vector suitable for gene silencing of stropharia rugoso-annulata is constructed, and meanwhile, a stropharia rugoso-annulata efficient genetic transformation system is established; by utilizing the silent vector and the transformation system established by the invention, the expression quantity of the target gene can be reduced by about 80%, the purpose of gene function research is achieved, and the silent vector and the transformation system have very strong theoretical research and application values.

The present invention discloses the application of the NtIPMD gene in regulating the chlorophyll content of tobacco, and belongs to the field of plant genetic engineering technology. Based on the existing progress in tobacco genetic engineering and the observation and identification of related plant phenotypes, the present invention conducts in-depth research on the NtIPMD gene and finds that it is related to the development of tobacco chloroplasts. The nucleotide sequence of the NtIPMD gene is shown in SEQ ID NO.1. After silencing the gene, the chlorophyll content of the leaves decreases, the growth of the plants slows down, and transmission electron microscopy observation reveals abnormal chloroplast development. The present invention finds that the gene encoding tobacco isopropylmalate dehydrogenase, NtIPMD, plays a key role in regulating the chlorophyll content of tobacco leaves, laying the foundation for clarifying the molecular regulatory mechanism of chlorophyll biosynthesis in tobacco leaves and providing a new precise regulatory gene for regulating the chlorophyll content of tobacco leaves.

The invention discloses an application of a PnMYB8 transcription factor in regulation and control of biosynthesis of dencichine. According to the present invention, the first research results show that when the PnMYB8 gene is silenced, the dencichine content in the dencichine synthesis plant such as panax notoginseng is significantly increased, and when the PnMYB8 gene is overexpressed, the dencichine content in the dencichine synthesis plant such as panax notoginseng is significantly reduced, such that the PnMYB8 transcription factor can positively regulate the biological synthesis of the dencichine; regulation of the PnMYB8 transcription factor on dencichine biosynthesis comprises two aspects: application of a PnMYB8 expression promoter in promoting dencichine biosynthesis (that is, the PnMYB8 expression promoter is adopted to promote dencichine biosynthesis), and application of a PnMYB8 silencing agent in inhibiting dencichine biosynthesis (that is, the PnMYB8 silencing agent is adopted to inhibit dencichine biosynthesis).

The invention relates to the technical field of plant genetic engineering, and particularly provides cloning of a GbGELP25D gene of gossypium barbadense and application of the GbGELP25D gene of gossypium barbadense. According to the invention, the GbGELP25D gene with a full length of 1092 bp is cloned from the sea island cotton variety Xinhai No. 7 for the first time, and the gene encodes a secretory lipase protein with a signal peptide and is positioned in an extracellular gap. Through bioinformatics analysis, phylogenetic classification, protein structure modeling and signal peptide function verification, it is clear that the gene belongs to a plant GELP family. Furthermore, a virus-induced gene silencing technology is utilized to prove that the silent GbGELP25D can obviously enhance the resistance of cotton to verticillium wilt, and the mechanism of the silent GbGELP25D is closely related to activation of ethylene synthesis and signal channels and induction of expression of disease-resistant related genes. The resistance gene provided by the invention enriches gene resources of cotton verticillium wilt resistance breeding, and has important theoretical significance and application value.

The application provides a plant antiviral gene XIAN and application thereof in antiviral, and belongs to the field of functional gene technology. The application provides a plant antiviral gene, wherein the plant antiviral gene comprises a conservative domain, the conservative domain is a MAM33 motif, or a conservative domain with more than 80% homology with the MAM33 motif. The application discloses Atxian, NbXIAN, SlXIAN, GmXIAN and GhXIAN genes all containing the MAM33 motif, in corresponding plants, the virus resistance of the mutants after silencing of the genes is weakened, and the virus resistance is improved after overexpression, which proves that the plant antiviral gene can significantly regulate the antiviral phenotype of the plant, so that germplasm with different antiviral phenotypes is created.

The application discloses RhALEU protein and an application of an encoding gene thereof in regulation of old age of rose petals. RhALEU The application screens out a gene from a rose transcriptome database RhALEU , and finds that expression of the gene is induced in the whole petal development stage. RhALEU The application verifies that the gene has a function of promoting old age of rose petals by using VIGS technology. RhALEU Silencing of the gene slows down the speed of the petal from the 5th grade to losing ornamental value. RhALEU In the petal with the silenced gene, expression of the old age related gene SAG12 is significantly down-regulated. RhALEU The application discloses the function of the gene in natural old age of the petal, provides a new thought and technical means for regulating old age of a plant flower, especially postharvest preservation of fresh cut rose, opens up a new research field of flower regulation, and has great academic value and important production application prospect.

This invention belongs to the field of plant genetic engineering technology, specifically relating to the application of H3K27me3 in regulating the cotton response to potassium chloride stress. By integrating CUT & Tag chromatin analysis and RNA-seq, this invention demonstrates that potassium chloride stress induces a reduction in H3K27me3 deposition across the entire genome, accompanied by characteristic stress phenotypes in cotton seedlings. Inhibition of H3K27me3 using RDS 3434 significantly improved KCl-induced physiological damage, confirming the functional correlation between this epigenetic marker and stress tolerance. Furthermore, virus-induced gene silencing confirmed that genes associated with H3K27me3 are important components of the cotton ion stress response network. Therefore, this invention elucidates the epigenetic landscape regulating adaptation to potassium chloride stress.

The invention discloses an inflorescence gene silencing method which comprises the following steps: firstly, synthesizing a specific nucleotide fragment according to a target gene, and constructing the specific nucleotide fragment to a plant virus vector; introducing the constructed plant virus vector and the corresponding helper plasmid into the inflorescence; soaking the inflorescence in a metal chelating agent salt solution; and finally, screening to obtain plants with inflorescences subjected to target gene silencing. The chrysanthemum capitulum is selected as an infection object, the silencing method is optimized, the target gene silencing efficiency is remarkably improved, the phenotype detection period after infection is remarkably shortened, and the method is efficient, convenient and high in operability. Meanwhile, the method can effectively avoid the cross protection phenomenon of plants, overcomes the obstacle of limited transmission range of viral vectors, does not need to consider the problems of death and growth cycle of tissue culture seedlings or seedlings after infection, and provides a feasible means for rapid identification of chrysanthemum gene functions and large-scale development of chrysanthemum gene function research.