19 results about "Silencing gene" patented technology

The present disclosure provides Crispr / cas9-based repressors for silencing gene targets in vivo and methods of use.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The application discloses RhALEU protein and an application of an encoding gene thereof in regulation of old age of rose petals. RhALEU The application screens out a gene from a rose transcriptome database RhALEU , and finds that expression of the gene is induced in the whole petal development stage. RhALEU The application verifies that the gene has a function of promoting old age of rose petals by using VIGS technology. RhALEU Silencing of the gene slows down the speed of the petal from the 5th grade to losing ornamental value. RhALEU In the petal with the silenced gene, expression of the old age related gene SAG12 is significantly down-regulated. RhALEU The application discloses the function of the gene in natural old age of the petal, provides a new thought and technical means for regulating old age of a plant flower, especially postharvest preservation of fresh cut rose, opens up a new research field of flower regulation, and has great academic value and important production application prospect.

Provided herein are compositions, systems, and methods for the creation, identification, and characterization of effector domains for activating and silencing gene expression. In particular, a high-throughput system for discovering and characterizing effector domains is provided.

This invention discloses NtBRL2 The application of genes in regulating potassium content in plants belongs to the field of plant genetic engineering technology. This invention provides a gene that can effectively regulate potassium content in plant leaves, a feature of existing technologies. NtBRL2 This invention first discovered through Real-time PCR. NtBRL2 The gene was expressed at the highest level in tobacco roots; then, a silenced gene was constructed using virus-induced gene silencing (VIGS) technology. NtBRL2 The VIGS vector of the gene was transformed into Nicotiana benthamiana, and repression was successfully achieved. NtBRL2 Silent plants, obtained by silencing gene expression, exhibit a specific phenotype with significantly lower potassium ion content compared to control plants, indicating that silencing this gene can significantly reduce potassium ion content in plants. This invention lays the foundation for elucidating the biological regulation mechanism of potassium ions and provides precise regulatory sites for the regulation of potassium ion content in plants.

A host cell comprising a first vector comprising a DNA comprising a nucleotide sequence encoding an adeno-associated virus (AAV) receptor (AAVR) or a variant thereof comprising AAVR biological activity; and a second vector comprising a DNA comprising a nucleotide sequence encoding a target gene tagged with a reporter gene is provided. A cell line comprising a plurality of the host cells comprising a first vector comprising a DNA comprising a nucleotide sequence encoding an adeno-associated virus (AAV) receptor (AAVR) or a variant thereof comprising AAVR biological activity; and a second vector comprising a DNA comprising a nucleotide sequence encoding a target gene tagged with a reporter gene is provided. A method for measuring the potency of a silencing gene therapy, the method comprising: transducing a host cell comprising a first vector comprising a DNA comprising a nucleotide sequence encoding an adeno-associated virus (AAV) receptor (AAVR) or a variant thereof comprising AAVR biological activity; and a second vector comprising a DNA comprising a nucleotide sequence encoding a target gene tagged with a reporter gene with an AAV comprising a DNA comprising a nucleotide sequence that inhibits expression of the target gene; measuring reporter gene expression in the host cell and a control; and determining a level of RNA inhibition based upon the difference in reporter gene expression in the host cell and the control is provided. In some aspects, the method is used as a potency assay to measure the potency of the AAV gene therapy product and / or measure the strength and stability of a therapeutic AAV product over time and under different storage conditions.

The application discloses an application of a leucine-rich repeat receptor-like kinase gene of Gossypium anomalum, which is composed of 3885 bases, wherein the first base from the 5' end is a transcription start site, and the 3885th base is a transcription termination site. Expression of the gene can cause a lethal phenotype of a single segment introgression line CSSL11-9 of the Gossypium anomalum. The lethal phenotype is inhibited by silencing the gene expression in the single segment introgression line CSSL11-9 of the Gossypium anomalum, and the single segment introgression line CSSL11-9 can survive normally. In addition, the gene can also endow cotton with resistance to verticillium wilt and fusarium wilt, and the resistance of the CSSL11-9 plant with the silenced gene to the cotton verticillium wilt and fusarium wilt is significantly reduced, thereby providing an important gene resource for cotton breeding against the verticillium wilt and the fusarium wilt.

This invention discloses an engineered bacterium producing tetracrine, its construction, and its application. The invention utilizes a strong promoter to drive the high expression of regulatory genes ovmZn and ovmWn in *Streptomyces neyagawa*, constructing a recombinant engineered strain Sne / pZWn; alternatively, it utilizes a strong promoter to drive the high expression of homologous genes of both, including the regulatory genes ovmZ and ovmW from *Streptomyces chromogenicus*, in *Streptomyces neyagawa*, constructing a recombinant engineered strain Sne / pZW. These engineered strains can express previously silenced gene clusters, activating tetracrine biosynthesis and laying the foundation for the development and application of tetracrine.

The invention relates to a strong promoter D8 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter D8 is as shown in SEQ ID No.1, and the strong promoter D8 comprises a plasmid vector of the strong promoter; the invention also discloses a host cell containing the plasmid vector, and application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, characterization of the promoter D8 provides a more effective tool element for streptomyces promoter engineering and high-efficiency gene expression, and the promoter D8 has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The present application belongs to the field of biotechnology, and specifically relates to a method for high-throughput screening of nucleic acid drug target genes, which comprises the following steps: treating cells with nucleic acid drug candidates or negative controls, and collecting measurement values for a plurality of cell characteristic parameters related to the treatment purpose of the nucleic acid drug; calculating the fold change of the characteristic parameter measurement values of the cells in each candidate hole relative to the negative control measurement values and normalizing them; for all measurement values, calculating the squared Mahalanobis distance of the plurality of cell characteristic parameters relative to the average value using the normalized values; screening out the candidates with squared Mahalanobis distance greater than a cutoff value, and unsupervised clustering the cells according to the plurality of cell characteristic parameters to divide the candidates into at least one cluster; obtaining the corresponding silenced genes of the candidates in each cluster, and performing gene ontology enrichment analysis on the genes in the cluster to obtain the biological processes associated with each cluster, and finally obtaining nucleic acid drug target genes for different biological processes.

Provided herein are methods for predicting tumor behavior and metastatic risk in carcinomas, the method comprising an analysis of RNA expression levels of a set of epigenetically activated genes and / or a set of epigenetically silenced genes. In some instances, the method further comprises administration of adjuvant therapy to a subject identified as having a high metastatic risk for a carcinoma.

The invention discloses a key gene for resisting paeonia lactiflora black spot by increasing lignin content and application of the key gene. The key gene is PlWAT1, the sequence of the key gene is as shown in SEQ ID NO.1, and a silent gene of the PlWAT1 gene is TRV-PlWAT1, and the sequence of the silent gene is as shown in SEQ ID NO.2. After the PlWAT1 gene is over-expressed, the plant height and the stem diameter of the tobacco are increased, and the xylem of the stem is widened; the silent gene can be used for regulating and controlling the growth of paeonia lactiflora, the plant height and stem diameter of the silenced paeonia lactiflora are reduced, the stem xylem width is narrowed, the scab area is larger after alternaria alternata is inoculated, and the enzyme activity is remarkably reduced. The plant height, the stem diameter, the lignin content and the black spot resistance of the Chinese herbaceous peony are regulated and controlled through overexpression or silencing genes, black spot resistant Chinese herbaceous peony varieties with stems different in height, thickness and hardness can be cultivated according to requirements, and therefore the method has important application value in the Chinese herbaceous peony molecular breeding process.

The application belongs to the grafting technical field, and particularly relates to application of a CmbHLH93 gene in melon and gourd grafting. The application provides a virus carrier for silencing a CmbHLH93 gene, and silences the CmbHLH93 gene of melon based on the carrier. The melon with the silenced gene is grafted with gourd, and graft compatibility is improved.

The present disclosure provides Crispr / cas9-based repressors for silencing gene targets in vivo and methods of use.

Provided herein are compositions, systems, and kits comprising effector domains for activating and silencing gene expression. In particular, synthetic transcription factors comprising the effector domains are provided.

A method of modifying a gene encoding or processed into a non-coding RNA molecule having no RNA silencing activity in a plant cell is disclosed. The method comprising introducing into the plant cell a DNA editing agent conferring a silencing specificity of the non-coding RNA molecule towards a target RNA of interest. A method of modifying a gene encoding or processed into a RNA silencing molecule in a plant cell is also disclosed. The method comprising introducing into the plant cell a DNA editing agent which redirects the silencing specificity of the non-coding RNA molecule towards a target RNA of interest. Plant cells, plant seeds, plants, and methods of generating plants are also disclosed.

The invention belongs to the technical field of biological agriculture, and particularly relates to an application of a gene StERF42 and an encoding protein thereof in improving potato late blight resistance. The invention finds that the gene StERF42 negatively regulates the resistance of potatoes to potato late blight pathogenic bacteria phytophthora infestans, and tests prove that the silent gene StERF42 down-regulates the expression of an SA signal channel marker gene StPR1, activates SA-dependent immune response, enhances active oxygen bursting, and further improves the resistance; and the overexpression gene StERF42 enables the susceptibility to be enhanced. A further research on a regulation mechanism shows that the gene StERF42 has transcriptional activation activity and can be combined with a GCC-box or GGCGGTG target motif to regulate and control downstream disease-resistant related genes. The invention provides a new target for potato disease resistance breeding, and has a wide application prospect in the aspect of potato late blight resistance improvement.

This invention belongs to the field of bio-agricultural technology, specifically relating to genes. StERF42 The invention relates to the application of its encoded protein in improving potato resistance to late blight. The gene discovered in this invention... StERF42 Negative regulation of potato resistance to Phytophthora in potato late blight pathogen was experimentally verified by silencing genes. StERF42 Downregulation of SA signaling pathway marker genes StPR1 The expression of this gene activates an SA-dependent immune response, enhances reactive oxygen species activity, and thus improves resistance; while overexpression of this gene... StERF42 This increases susceptibility to disease. Further research into the regulatory mechanisms revealed that genes... StERF42 StERF42 StERF42 StERF42 StERF42 StERF42 StERF42 St It possesses transcriptional activation activity and can bind to GCC-box or GGCGGTG target motifs to regulate downstream disease resistance-related genes. This invention provides a new target for potato disease resistance breeding and has broad application prospects in improving potato resistance to late blight.

The invention belongs to the technical field of biological agriculture, and discloses application of a gene TaToc34 and an encoding protein thereof in regulation and control of wheat stripe rust resistance. The nucleotide sequence of the gene TaToc34 is as shown in SEQ ID NO: 1, the amino acid sequence of the protein TaToc34 is as shown in SEQ ID NO: 2, tests verify that the gene TaToc34 is subjected to induced expression of stripe rust, an agrobacterium-mediated method is adopted to construct a gene silencing plant of the gene TaToc34, the gene TaToc34 plays a positive regulation role in immune response of wheat to stripe rust, the silencing gene TaToc34 reduces expression of wheat defensive genes, and the gene TaToc34 has a positive regulation role in immune response of wheat to stripe rust. The wheat stripe rust resistance is reduced, and a new gene resource is provided for improvement of the wheat stripe rust.