231 results about "Silent gene" patented technology

The invention discloses a wheat salt-tolerant gene and application thereof. Specifically, the invention discloses a protein TaSGR (SEQ ID NO: 3, 6 and / or 9) and an application of a coding gene of the protein TaSGR in regulation and control of plant salt tolerance and / or plant height. According to the invention, a homozygous gene editing plant of which the TaSGR gene is knocked out is successfully created by utilizing a CRISPR / Cas9 technology. The result shows that after the TaSGR gene is knocked out, the plant height of the wheat is obviously reduced, and the salt tolerance is obviously enhanced. The invention discloses the application of the protein TaSGR and the coding gene thereof for the first time. The salt tolerance of the plant is increased and / or the plant height of the plant is reduced by reducing the content and / or activity of the protein TaSGR (for example, the TaSGR gene is knocked out or silenced). According to the invention, a good gene resource is provided for salt-tolerant breeding of wheat, a new field is developed for application of the TaSGR gene, and the TaSGR gene has important significance for cultivation of novel salt-tolerant wheat.

The invention discloses an application of an RcWRKY31 gene in enhancing the resistance of Chinese rose to gray mold. The nucleotide sequence of the RcWRKY31 gene is as shown in SEQ ID NO. 1. The RcWRKY31 gene is silenced and overexpressed in petals which are good in Chinese rose growth state and free of botrytis cinerea infection, and research results show that the petals treated by the silent RcWRKY31 gene are larger in scab area and lower in resistance to botrytis cinerea; the resistance of petals treated by overexpression of the RcWRKY31 gene to gray mold is enhanced.

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention belongs to the technical field of gene engineering, and discloses a wheat susceptibility related gene, a protein and application. The wheat susceptibility related gene is a TaBRG10 gene and is derived from a wheat variety water source 11, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the TaBRG10 gene is as shown in SEQ ID NO: 2. The TaBRG10 gene is induced to express by stripe rust, and under the infection of stripe rust, the infection surface of a TaBRG10 gene silent plant infected by stripe rust is positively and obviously lower than that of a control plant, which indicates that the silent TaBRG10 gene improves the resistance of the plant to wheat stripe rust. The invention provides a novel germplasm material for the cultivation of the stripe rust resistant wheat variety.

The invention discloses application of an ESP1 gene in regulating the resistance of plants to rice blast, and belongs to the technical field of molecular biology. The invention provides the application of the ESP1 gene in regulating the resistance of the plant to the rice blast, and experiments prove that a mutant plant with a silent ESP1 gene is susceptible to the rice blast, and a mutant plant with an overexpressed ESP1 gene is resistant to the rice blast. The invention is expected to provide a good theoretical basis for cultivating disease-resistant plants.

The present disclosure provides Crispr / cas9-based repressors for silencing gene targets in vivo and methods of use.

The invention provides application of an Aars1 gene in regulation and control of expression of an exogenous recombinant protein, and belongs to the technical fields of recombinant protein expression, gene engineering and cell culture. According to the CHO cell line, shRNA fragments of targeted Aars1 genes are inserted and integrated into piggyBac transposon vectors, a recombinant vector for inhibiting / silencing the Aars1 genes and an Aars1 low-expression CHO cell line are constructed, the CHO cell line can remarkably reduce the mRNA expression level of the Aars1, meanwhile, the CHO cell line serves as a host cell for recombinant protein expression, and compared with a wild type CHO cell line, the CHO cell line has the advantages that the expression level of the CHO cell line is greatly improved; the recombinant adalimumab can improve the expression quantity of recombinant protein (the expression quantity of the recombinant adalimumab is increased by 3.1 + / -0.18 times), effectively overcomes the problem of low expression level of the existing CHO cell expression system, and can be used for further constructing a recombinant protein expression system or kit.

The invention discloses an application of a tomato Solyc04g064880 gene in regulation and control of ToBRFV resistance of a plant, a Solyc04g064880 gene silencing vector pTRV2 is constructed: S14880, agrobacterium tumefaciens is transformed, positive bacteria obtained by screening are inoculated into a target plant to obtain a Solyc04g064880 gene silencing strain, and compared with a contrast, the Solyc04g064880 gene silencing strain has the advantages that the ToBRFV accumulation amount in the plant is reduced, and the ToBRFV resistance of the plant can be regulated and controlled. Therefore, the silent Solyc04g064880 gene can improve the resistance of the plant to the ToBRFV, and a technical support is provided for the breeding of a ToBRFV resistant variety of the plant.

The invention discloses application of an RRM1-61 gene in regulation of rice blast resistance of plants, and belongs to the technical field of gene engineering. The invention provides the application of the RRM1-61 gene in regulating the rice blast resistance of plants, and tests prove that the plants with silent RRM1-61 gene are susceptible diseases, the leaf scab area is significantly different from that of a wild type, and the leaves except the scab are also yellowed. The plant over-expressed with the RRM1-61 gene is more disease-resistant, and compared with a wild type, the plant over-expressed with the RRM1-61 gene has significantly different disease spot areas on leaves. The invention provides a technical basis for cultivating a new variety of rice blast resistance of plants, and has a wide application prospect.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention provides a method for inducing synthesis of capsaicin by using an optical signal transcription factor CaBBX2, and particularly relates to an experimental method for the influence of a living plant silencing CaBBX2 gene on synthesis of capsaicin. Capsicum fruits after pollination for 16 days, 25 days, 33 days and 38 days are taken to observe and analyze the content of capsaicin, dihydrocapsaicin and total capsaicin and the change condition of capsaicin synthesis related gene expression, and experimental results show that after the CaBBX2 gene is silenced, the content of capsaicin is remarkably reduced relative to that of a no-load control group, which indicates that the CaBBX2 and the biosynthesis of capsaicin have a certain correlation, and along with the silencing of the CaBBX2, the content of capsaicin is remarkably reduced relative to that of a no-load control group. The expression quantity of the capsaicin synthesis related gene is also reduced to different degrees, and further verification shows that the CaBBX2 can influence the synthesis of capsaicin by regulating the expression of the capsaicin synthesis related gene.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a TRIM3 gene or protein serving as a target spot in screening of drugs for inhibiting foot and mouth disease virus replication. The method specifically comprises the following steps: firstly, constructing a TRIM3 knockout cell line by utilizing a CRISPR / Cas9 system, wherein the cell line obviously inhibits the replication of FMDV; secondly, according to the invention, a TRIM3 stable expression cell line is constructed by using a lentivirus packaging system, and the cell line promotes the replication of FMDV. Therefore, the inhibition or silencing of the TRIM3 gene / protein can inhibit the replication of the foot-and-mouth disease virus, can be used as a target spot for screening drugs for inhibiting the replication of the foot-and-mouth disease virus, provides some theoretical bases for preventing or inhibiting FMDV infection in the future, and has a wide application prospect.

The present invention relates to the field of genetic engineering technology, in particular to taro CePDS Gene and VIGS virus vector and silencing method thereof. The present invention provides a taro phytoene dehydrogenase, the amino acid sequence of which is shown in SEQ ID NO.1. The present invention discovered the amino acid sequence of taro phytoene dehydrogenase and its encoding gene CePDS sequence, on this basis, provides a kind of silencing CePDS The target sequence of the gene, the VIGS silencing system and the silencing method can effectively reduce the CePDS The expression level of the gene can enable the VIGS silencing system to spread systemically in taro plants, thereby effectively interfering with the CePDS The VIGS silencing system and method provided by the present invention will greatly improve the progress of taro gene function research and significantly save time and cost.

The invention discloses a specific saliva gene NlSP55 of brown planthopper and application of the specific saliva gene NlSP55 in biological control of brown planthopper. Wherein the nucleotide sequence of the nilaparvata lugens saliva gene NlSP55 is as shown in SEQ ID NO. 1, and the coded amino acid sequence is as shown in SEQ ID NO. 2. The dsRNA of the synthesized saliva gene NlSP55 is introduced into the brown planthopper body by using an RNA interference technology, so that the NlSP55 gene can be silent in a targeted manner, the feeding capacity of the brown planthopper is inhibited, the survival rate of the brown planthopper on sensitive rice and resistant rice is quickly reduced, and finally, the biological control effect of the brown planthopper is achieved. The invention specifically aims at the brown planthopper, is harmless to mammals, fishes and shrimps, natural enemy insects and pollination insects, and provides a new way for preventing and treating the brown planthopper.

The invention relates to the technical field of plant genetic engineering, and provides a receptor-like kinase gene for improving Orobanche coerulescens parasitism resistance of sunflower and application of the receptor-like kinase gene. The invention discovers an amino acid sequence of the sunflower receptor kinase and a nucleotide sequence of the coding gene HaCERK1, and provides a target sequence for silencing the HaCERK1 gene, a virus-mediated gene silencing system and a silencing method based on the amino acid sequence, so that the expression level of the sunflower receptor kinase HaCERK1 gene can be effectively inhibited, and the expression level of the sunflower receptor kinase HaCERK1 gene can be effectively inhibited. And a virus-mediated gene silencing system can be systematically diffused in sunflower and sunflower orobanche tissues, so that the expression of the HaCERK1 gene is effectively interfered. According to the virus-mediated gene silencing system and method provided by the invention, the sunflower gene function research efficiency is remarkably improved, and the time and the cost are greatly reduced.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a DcTH gene in prevention and control of diaphorina citri, and the nucleotide sequence of the DcTH gene is shown as SEQ ID NO.1. The tyrosine hydroxylase DcTH gene is accurately identified and screened by taking diaphorina citri as a research object. Further, dsRNA is successfully synthesized by designing a specific primer, and an RNA interference technology is applied to the expression of the DcTH gene. Researches find that after the DcTH gene is silenced, the key physiological process of ecdysis of the diaphorina citri is interfered, the malformation rate and the death rate are remarkably increased, and finally effective prevention and control of the diaphorina citri are achieved. The DcTH gene provided by the invention can further provide a brand-new molecular target and theoretical support for development of a diaphorina citri prevention and control technical means based on RNAi (Ribonucleic Acid Interfere).

The invention relates to a cotton leaflet character regulation gene and an encoded protein and application thereof, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2. According to the invention, a gene capable of regulating and controlling the leaf rolling character of cotton is obtained by cloning from the goblet oilless cotton and is named as a Ghcup gene, and silencing research is carried out on the Ghcup gene in upland cotton goblet oilless cotton by constructing a VIGS vector of the Ghcup gene of the cotton, so that the Ghcup gene can be used for regulating and controlling the leaf rolling character of the cotton. A cotton Ghcup gene overexpression vector is constructed, transgenic overexpression is carried out in normal broad-leaf upland cotton Xinluzhong 75, and results show that the curly phenotype of an oilless goblet-leaf cotton leaf of a silent Ghcup gene disappears, the overexpression of the gene causes the upward curly shape of the Xinluzhong 75 cotton leaf to be similar to a cup shape, and the cotton Ghcup gene overexpression vector is used for transgenosis overexpression in the normal broad-leaf upland cotton Xinluzhong 75. Therefore, the Ghcup gene has great application potential in the aspects of improving the leaf shape and the plant canopy structure of the cotton, improving the photosynthetic efficiency of the plant and the like.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The invention relates to the technical field of molecular breeding, and discloses an auxin amide hydrolase RhILL1 gene and application of the auxin amide hydrolase RhILL1 gene in regulation and control of China rose flower color, and the nucleotide sequence of the auxin amide hydrolase RhILL1 gene is shown as SEQ ID NO.1. By silence or overexpression of the RhILL1 gene, synthesis of anthocyanin is effectively regulated and controlled, and accurate control of petal color is realized. Researches find that silencing of the RhILL1 gene leads to color fading of petals and reduction of anthocyanin content; and overexpression of the RhILL1 gene prolongs the color holding time of the petals. In addition, the invention also provides a kit containing the RhILL1 gene regulatory sequence, a recombinant plasmid and a corresponding gene editing and transgenic breeding method. The achievements provide a new molecular tool for Chinese rose breeding and flower color improvement, and have important ornamental value and economic significance.

The invention discloses a silence system of a potato transcription factor StLRP1 gene and application of the silence system, and aims to regulate formation and development of potato tubers by silence of the StLRP1 gene so as to improve the yield and quality of the potato tubers. By silencing the StLRP1 gene, the tuberization efficiency of potatoes is remarkably improved, the tuberization time is advanced, early maturing is promoted, and the planting period is shortened. Meanwhile, the growth period is shortened due to gene silencing, the planting efficiency is enhanced, and the potatoes are more suitable for short growth seasons. In addition, the tubers of the plants are changed from an ovulate shape to a long cylinder shape, and the weight of a single potato plant is reduced, so that the storage and transportation efficiency can be improved, the market preference can be met, more choices are provided for breeding, and the cultivation of potato varieties adapting to different conditions is facilitated. Generally, the present invention enhances the production and adaptability of potatoes and brings advantages to the entire production chain of potatoes by improving the form and weight of tubers.

The invention provides a jasmine VIGS silencing system as well as a construction method and application thereof, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: firstly, providing a specific nucleotide fragment for silencing the arabian jasmine flower PDS gene, and inserting the specific nucleotide fragment into a pTRV2 vector through homologous recombination by taking a tobacco embrittlement virus (TRV) as the vector, so as to construct a silencing vector pTRV2-JsPDS; a vector pTRV1, a vector pTRV2 and a silence vector pTRV2-JsPDS are respectively transferred into agrobacterium to obtain a VIGS silence system of the jasmine flower PDS gene, the silence system is adopted to infect a jasmine flower plant, experiments prove that the silence system can induce silence of the jasmine flower JsPDS gene, the total chlorophyll content in jasmine flower leaves after gene silence is reduced by 34.98-63.79%, and the total chlorophyll content in the jasmine flower leaves after gene silence is reduced by 34.98-63.79%. And the relative expression quantity of the endogenous JsPDS gene is reduced by 56.38%-85.79%. The method has the advantages of being easy and convenient to operate, short in period, high in silencing efficiency, free of complete gene sequence information and genetic transformation systems and the like, and an efficient and reliable technical means is provided for arabian jasmine flower gene function research.

The invention relates to the technical field of gene engineering, in particular to a cynanchum thesioides CtPDS gene as well as a VIGS virus vector and a silencing method of the cynanchum thesioides CtPDS gene. The invention provides phytoene dehydrogenase of cynanchum thesioides. The phytoene dehydrogenase has an amino acid sequence as shown in SEQ ID NO. 1. The invention discloses an amino acid sequence of cynanchum thesioides phytoene dehydrogenase and a sequence of a coding gene CtPDS of the cynanchum thesioides phytoene dehydrogenase, and provides a target nucleic acid molecule for silencing the CtPDS gene, a VIGS silencing system and a silencing method on the basis of the amino acid sequence of the cynanchum thesioides phytoene dehydrogenase. The leaf CtPDS gene expression level of a CtPDS gene silent plant constructed by the method provided by the invention is remarkably reduced, the silence efficiency reaches 56-65%, the chlorophyll and carotenoid contents are remarkably reduced, the leaf has a whitening phenotype, and a technical support is provided for developing functional research of cynanchum thesioides key genes.

The invention discloses an application of a ClBZR1L gene in enhancing the cold resistance of watermelons by promoting proline accumulation. The invention belongs to the technical field of plant genetic engineering. The CDS sequence of the ClBZR1L gene is as shown in SEQ ID NO. 1. Experiments show that when a recombinant vector for constructing the silent ClBZR1L gene is transferred into a watermelon plant, the proline content of the silent plant under cold stress treatment is remarkably reduced compared with that of a control group, and the cold resistance is reduced. A recombinant vector for constructing the over-expression ClBZR1L gene is transferred into a watermelon plant, the proline accumulation amount of the over-expression plant under cold stress treatment is remarkably increased, the cold resistance is enhanced, and a gene resource is provided for watermelon cold-resistant molecular breeding. Besides, the invention also discloses direct combination of the ClBZR1L gene and a proline synthesis rate-limiting enzyme coding gene ClP5CS1 gene promoter, and the expression of the coding gene is up-regulated, so that synthesis of proline under cold stress is promoted, and a theoretical basis is provided for in-depth study on cold-resistant gene pathways.

The invention relates to an shRNA (short hairpin Ribonucleic Acid) interference sequence of a targeted silence PCSK9 gene and a construction method and lipid-lowering application of a recombinant adeno-associated virus vector of the shRNA interference sequence. Hyperlipidaemia is a metabolic disease characterized by abnormal rising of cholesterol and triglyceride levels in blood, and the design of lipid-lowering drugs is the focus of attention to improvement of hyperlipidaemia. Proprotein convertase subtilisin / kexin type 9 (PCSK9) can be combined with a low-density lipoprotein receptor (LDL-R) and degrade the LDL-R, so that accumulation of LDL-C in blood is further promoted, and hyperlipidemia is caused. Aiming at the key target PCSK9, a specific shRNA interference sequence is designed, and a recombinant adeno-associated virus vector (rAAV) carrying the sequence is constructed by an enzyme digestion-connection method. In-vitro experiments prove that the vector can remarkably reduce the expression level of PCSK9 protein, so that the cyclic utilization of a low-density lipoprotein receptor (LDL-R) is promoted, and the concentration of low-density lipoprotein cholesterol (LDL-C) in plasma is reduced. The rAAV vector provided by the invention has the characteristics of low production cost, high transfection efficiency, lasting action time and the like, and provides a new thought for gene therapy of hyperlipidemia.

The invention relates to the technical field of agricultural bioengineering, and particularly provides application of a rice CONSTANS-LIKE 5 gene and an interaction protein OsIDS1 thereof in regulating and controlling the heading stage of rice. The CONSTANS-LIKE 5 gene is cloned from rice Nipponbare NIP, is a transcription factor protein of 372 amino acids, and belongs to a CCT / B-box ginc finger protein family. Genetic transformation experiments prove that the overexpressed CONSTANS-LIKE 5 gene promotes the heading of the rice under a short-day condition, and the knockout or silencing CONSTANS-LIKE 5 gene inhibits the heading of the rice under the short-day condition; overexpression of the OsIDS1 gene inhibits rice heading under a short-day condition, and knockout or silencing of the OsIDS1 gene promotes rice heading under the short-day condition.

This invention relates to the fields of molecular biology and biomedicine, and discloses siRNA for the expression of the *Schistosoma japonicum* Hsc20 gene and its applications. The siRNA contains a nucleotide sequence that hybridizes to a target sequence of the *Schistosoma japonicum* Hsc20 gene, wherein the target sequence is selected from the sequences shown in SEQ ID NO. 1-SEQ ID NO. 4. The siRNA specifically inhibiting the expression of the *Schistosoma japonicum* Hsc20 gene provided by this invention can be used to interfere with the transcription and expression of the *Schistosoma japonicum* Hsc20 gene and the growth and development of *Schistosoma japonicum*. In vitro experiments have confirmed that the siRNA provided by this invention can efficiently silence the *SjHsc20* gene; and in vivo RNA interference experiments in mice show that this siRNA can induce 50.62% (…) P The insect reduction rate was <0.05% and 44.29% ( P A liver oocyte reduction rate of <0.01% is suitable for preparing drugs to treat schistosomiasis.

A method for modifying a gene encoding or processed to a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell is disclosed, with the proviso that the eukaryotic cell is not a plant cell. The method comprises introducing a DNA editor into the eukaryotic cell, the DNA editor conferring a silence specificity of the non-coding RNA molecule for a target RNA of interest. Also disclosed is a method of modifying a gene for encoding or being processed into an RNA silencing molecule for a target RNA in a eukaryotic cell. Also disclosed are methods of preventing and treating various diseases, methods of inducing apoptosis, and methods of producing an eukaryotic non-human organism.

The application discloses a kiwi bacterial canker disease susceptible gene AcADC1 and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the AcADC1 gene is shown in SEQ ID NO:1, and the arginine decarboxylase (ADC) synthesized by the gene is encoded, and the amino acid sequence is shown in SEQ ID NO:2, and the arginine (Arg) is responsible for catalyzing the formation of putrescine. The resistance of the kiwi plant with the silenced AcADC1 gene to the bacterial canker disease is improved, the resistance of the kiwi plant with the overexpressed AcADC1 gene to the bacterial canker disease is reduced, the AcADC1 gene plays a negative regulation role in the immune response of the kiwi to the bacterial canker disease, the gene editing technology can be used for editing the AcADC1 gene, and a theoretical basis and gene reserve are provided for kiwi disease-resistant breeding.

Provided herein are compositions, systems, and methods for the creation, identification, and characterization of effector domains for activating and silencing gene expression. In particular, a high-throughput system for discovering and characterizing effector domains is provided.

The invention relates to the technical field of biology, in particular to application of an OsY37 gene to regulation and control of stigma vitality of a rice sterile line. According to the present invention, the rice sterile line stigma vitality is improved by knocking out or silencing the OsY37 gene, or the rice sterile line stigma vitality is reduced by up-regulating the expression level of the OsY37 gene, the DNA sequence of the OsY37 gene is represented by SEQ ID NO.1, and the knockout or OsY37 gene can effectively improve the rice sterile line stigma vitality so as to effectively prolong the pollination period of the rice stigma and improve the hybrid rice seed yield.