79 results about "Silent gene" patented technology

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

This invention relates to the fields of molecular biology and biomedicine, and discloses siRNA for the expression of the *Schistosoma japonicum* Hsc20 gene and its applications. The siRNA contains a nucleotide sequence that hybridizes to a target sequence of the *Schistosoma japonicum* Hsc20 gene, wherein the target sequence is selected from the sequences shown in SEQ ID NO. 1-SEQ ID NO. 4. The siRNA specifically inhibiting the expression of the *Schistosoma japonicum* Hsc20 gene provided by this invention can be used to interfere with the transcription and expression of the *Schistosoma japonicum* Hsc20 gene and the growth and development of *Schistosoma japonicum*. In vitro experiments have confirmed that the siRNA provided by this invention can efficiently silence the *SjHsc20* gene; and in vivo RNA interference experiments in mice show that this siRNA can induce 50.62% (…) P The insect reduction rate was <0.05% and 44.29% ( P A liver oocyte reduction rate of <0.01% is suitable for preparing drugs to treat schistosomiasis.

Provided herein are compositions, systems, and methods for the creation, identification, and characterization of effector domains for activating and silencing gene expression. In particular, a high-throughput system for discovering and characterizing effector domains is provided.

The invention relates to the technical field of biology, in particular to application of an RhUNE10 gene to regulation and control of root cancer resistance of Chinese roses and a regulation and control method. The nucleotide sequence of the RhUNE10 gene is as shown in SEQ ID NO. 1. The application mode is that the RhUNE10 gene is silenced and knocked out, or the expression of the protein coded by the RhUNE10 gene is inhibited; and the amino acid sequence of the protein coded by the RhUNE10 gene is as shown in SEQ ID NO. 3. After the RhUNE10 gene is silenced, the resistance of the Chinese rose to agrobacterium tumefaciens is remarkably reduced, which indicates that the RhUNE10 gene has important biological functions, is beneficial to promoting Chinese rose functional genomics research, gene editing breeding and transgenic breeding research, excavates and cultivates new varieties of high-resistance Chinese roses, and has wide application prospects. And a certain theoretical basis and key gene resources are provided for comprehensive prevention and treatment of root cancer and cultivation of a new variety of Chinese rose.

The invention belongs to the field of biological medicine, and particularly relates to a YBX3 gene, a YBX3 gene inhibitor and application of the YBX3 gene inhibitor. The invention discloses application of the YBX3 gene as a biomarker in preparation of a product for detecting cardiac fibrosis. The nucleotide sequence of the YBX3 gene is as shown in SEQ ID NO. 1. The invention discloses application of a YBX3 gene inhibitor in preparation of drugs for preventing and / or treating heart diseases. The YBX3 gene inhibitor is shRNA (short hairpin ribonucleic acid) and has a nucleotide sequence as shown in SEQ ID NO. 3. When myocardial infarction occurs, mRNA and protein level expression activity of the YBX3 gene is remarkably improved and is related to fibroblast activation, and the silent YBX3 gene can remarkably inhibit fibroblast activation. The existing myocardial infarction injury medicine has a limited treatment effect, so that the YBX3 can improve the clinical prognosis of myocardial infarction injury by regulating and controlling the activation of cell fibrosis.

This invention discloses NtBRL2 The application of genes in regulating potassium content in plants belongs to the field of plant genetic engineering technology. This invention provides a gene that can effectively regulate potassium content in plant leaves, a feature of existing technologies. NtBRL2 This invention first discovered through Real-time PCR. NtBRL2 The gene was expressed at the highest level in tobacco roots; then, a silenced gene was constructed using virus-induced gene silencing (VIGS) technology. NtBRL2 The VIGS vector of the gene was transformed into Nicotiana benthamiana, and repression was successfully achieved. NtBRL2 Silent plants, obtained by silencing gene expression, exhibit a specific phenotype with significantly lower potassium ion content compared to control plants, indicating that silencing this gene can significantly reduce potassium ion content in plants. This invention lays the foundation for elucidating the biological regulation mechanism of potassium ions and provides precise regulatory sites for the regulation of potassium ion content in plants.

The invention discloses application of expression of a targeted silence TdSb-sbd gene in increasing the egg laying amount of female trichogramma dendrolimus, and belongs to the technical field of biological prevention and control. The invention discovers that the SPS protein (coding gene TdSb-sbd) of trichogramma dendrolimus can interact with the juvenile hormone degradation key enzyme JHEH1 protein. After the TdSb-sbd gene is silent in a targeted manner, the expression level of the gene can be reduced, and the expression of an interaction protein coding gene Tdjheh1 of the gene is reduced, so that the degradation pathway of juvenile hormone (JH) is inhibited, and the titer of juvenile hormone in trichogramma is improved. The high juvenile hormone level can promote ovarian development and ovum generation of trichogramma, and finally the egg laying amount of female bees is remarkably increased. The trichogramma reproductive capacity is directly regulated and controlled through a molecular biology means, and a new technical scheme is provided for improving the trichogramma dendrolimus reproductive capacity and the field pest control efficiency on a large scale.

This invention discloses the application of platelet-reactive protein-4 (THBS4) in the diagnosis and treatment of endometriosis. Through bioinformatics integration analysis, clinical sample validation, and in vitro and in vivo experiments, this invention confirms that THBS4 is significantly highly expressed in both ectopic lesions and peripheral blood of patients with endometriosis, and its expression level is positively correlated with disease severity. The area under the receiver operating characteristic (AUC) curve for THBS4 alone in diagnosing endometriosis is 0.930, significantly superior to cancer antigen 125 (CA125); the AUC for the combined diagnosis of THBS4 and CA125 reaches 0.968. Simultaneously, silencing the THBS4 gene effectively inhibits the proliferation, migration, and invasion of human endometrial stromal cells, and significantly reduces the volume and fibrosis area of ​​ectopic lesions in a mouse model of endometriosis. This invention provides a highly sensitive and specific new biomarker for the non-invasive or minimally invasive early diagnosis of endometriosis, and provides new targets and candidate drugs for non-hormone-dependent targeted therapy.

The application is suitable for the field of biological medicine technology, and provides a siRNA-PSMA conjugate targeting a LEPR gene, a preparation method and application thereof. The siRNA-PSMA conjugate realizes specific targeted delivery of CRPC cells through a PSMA inhibitor target head, effectively reduces off-target toxicity, combines with a high-interference-efficiency M3 modified LEPR siRNA screened, can efficiently silence LEPR gene expression, block a leptin-LEPR signal pathway, and then inhibit CRPC cell proliferation, invasion and drug resistance related biological functions; the preparation process is stable and controllable, the purity of the finished product after purification can reach more than 95%, meets the quality requirements of drug development, and the activity verification method has strong specificity and reliable results, fills the blank of CRPC targeted therapy drugs in the prior art, provides a new precise targeting strategy for CRPC treatment, and has important clinical application value and broad conversion prospect.

The invention discloses application of a GhGDSL102 gene in regulating and controlling the oil content of a plant, and belongs to the technical field of gene engineering. The GhGDSL102 gene is over-expressed in arabidopsis thaliana, the oil content of arabidopsis thaliana seeds is obviously reduced by 13.67%, and oleic acid C18: 1 is obviously reduced by 7.7%. After the GhGDSL102 gene is silenced, the oil content and oleic acid content of the cotton seeds are obviously increased by 9.99% and 7.57%. Meanwhile, three transgenic positive strains are obtained through overexpression of cotton, it is found through measurement of the oil content of cotton seeds that the oil content of the three overexpression strains is remarkably reduced by 11.70%, 9.14% and 19.25% respectively compared with that of a control group, and the oleic acid content is reduced by 5.54%, 7.09% and 6.29% respectively; the cottonseed oil content of the obtained homozygous edited strain is remarkably improved by 19.4%, and the GhGDSL102 is proved to be a gene for negatively regulating the cottonseed oil content.

The invention discloses an application of OS1 protein and a coding gene thereof in regulating tassel development and grain development of plants. Specifically disclosed is the use of down-regulating the content and / or activity of protein OS1 (SEQ ID NO: 1) (e.g., knocking out or silencing OS1 gene) in altering tassel morphology, reducing grain length, increasing grain width, reducing grain embryo size, and / or increasing floret number of tassel principal axis. The invention discloses the effect of the OS1 gene and the encoded protein OS1 thereof in regulation and control of tassel development and grain development of corn for the first time. According to the invention, the wide-grain corn with increased floret number of the tassel main shaft is successfully created, and a material accumulation, efficient and safe technical method is provided for corn germplasm resource improvement and breeding. The invention provides a good gene resource for molecular breeding of corn, opens up a new field for application of the OS1 gene, and has important significance for cultivating novel corn and promoting the commercial breeding process of corn.

This invention relates to the field of bioengineering technology, and more particularly to a gene for identifying salt tolerance in upland cotton, GhNRX16, and its molecular marker. The CDS sequence of the upland cotton gene GhNRX16 is shown in SEQ ID NO.1 of the sequence listing. This invention uses RNA-seq technology to screen and identify this salt tolerance-related gene in the transcriptome data of upland cotton under salt stress treatment. Furthermore, the GhNRX16 sequence was obtained through gene cloning, and a transient silencing vector based on VIGS (virus-induced gene silencing) technology was successfully constructed. Experiments using VIGS-mediated gene silencing combined with salt stress treatment showed that silencing the GhNRX16 gene significantly reduced the plant's tolerance to salt stress, demonstrating that normal expression of this gene directly contributes to ensuring the plant's salt stress resistance. These results confirm the crucial role of the GhNRX16 gene in regulating cotton salt tolerance, providing important gene resources and theoretical basis for molecular breeding of salt-tolerant cotton.

The invention relates to the technical field of biology, in particular to a brown planthopper NlABCG1 gene, encoding protein thereof and application of dsRNA of the brown planthopper NlABCG1 gene in prevention and treatment of brown planthopper. The invention discloses an NlABCG1 gene. The function of the gene is inhibited, so that the survival rate of brown planthoppers is reduced; after the dsRNA of the NlABCG1 synthesized in vitro is used for silencing the NlABCG1 gene in a microinjection mode, the survival rate of the brown planthopper is remarkably reduced, and the shape and appearance of the brown planthopper are remarkably changed. The invention has a good application prospect, and is of great significance to prevention and treatment of brown planthopper.

The present application relates to the technical field of biological genetic engineering, and particularly relates to a kind of identification of plant salt tolerance land cotton gene GhNRX71 And molecular marker, the CDS sequence of the land cotton gene GhNRX71 As shown in SEQ ID NO.1 In the sequence table.The present application is screened and identified in the transcriptome data of land cotton under salt stress treatment by transcriptome sequencing (RNA-seq) technology.The sequence of GhNRX71 Is obtained by further gene cloning means, and the transient silencing vector based on VIGS (virus-induced gene silencing) technology is successfully constructed.The results show that the tolerance of plant to salt stress significantly reduces after silencing GhNRX71 Gene by using VIGS-mediated gene silencing combined with salt stress treatment experiment, which proves that the normal expression of the gene has a direct effect on guaranteeing the salt stress resistance of plant.The above results confirm that GhNRX71 Gene plays a key role in regulating the salt tolerance of cotton, and provides important gene resources and theoretical basis for cotton salt-tolerant molecular breeding.

This invention belongs to the field of biological control technology for brown planthoppers, specifically relating to the application of the Tip60 gene and its encoded protein in the control of brown planthoppers. This invention is the first to discover that the Tip60 gene plays a crucial role in the control of brown planthoppers. Further experiments confirmed that reducing the expression level of the Tip60 gene significantly shortens the survival time of female brown planthoppers and reduces the number of offspring, indicating that inhibiting Tip60 gene expression can effectively control brown planthoppers, providing a theoretical basis for this strategy. Simultaneously, this invention uses RNAi technology to interfere with the expression of this gene during the adult stage of brown planthoppers, producing a significant inhibitory effect, thus providing a novel strategy and target for brown planthopper control, and demonstrating the application prospects of RNAi technology in silencing the Tip60 gene in brown planthopper control.

The invention belongs to the technical field of biological medicines, and discloses an oligonucleotide molecule for targeted inhibition of a human LINC00921 gene, a coding oligopeptide and application of the oligonucleotide molecule in treatment of lung adenocarcinoma. The invention also discloses application of related drugs or products taking the oligonucleotide molecule for targeted inhibition of the human LINC00921 gene as an active ingredient in inhibition or treatment of lung adenocarcinoma. A specific siRNA target sequence is designed aiming at a target gene, a retrovirus vector plasmid containing the target sequence is successfully constructed through genetic engineering, a recombinant retrovirus capable of efficiently silencing the LINC00921 gene is obtained, the mRNA expression level of the LINC00921 gene in lung adenocarcinoma cells can be remarkably reduced, the gene knock-down effect is outstanding, and the retrovirus can be applied to lung adenocarcinoma cells. The compound has a remarkable inhibition effect on proliferation, migration and invasion ability of lung adenocarcinoma cells. Therefore, the invention has important application value in treatment of lung adenocarcinoma, and has high-throughput operability and good repeatability.

The application belongs to the technical field of genetic engineering, and particularly relates to application of TRIM25 gene as a target point in inhibition of H9N2 influenza virus replication.(1) The application finds that inhibition or silencing of the TRIM25 gene can inhibit replication of the H9N2 influenza virus, and TRIM25 can be used as a target point for screening drugs for inhibiting replication of the H9N2 influenza virus;(2) The application provides an sgRNA specifically targeting the TRIM25 gene, and complete knockout of the TRIM25 gene is realized by combining CRISPR-Cas9 technology, and a monoclonal cell line obtained has a resistance phenotype to the H9N2 influenza virus, thereby providing a new idea for prevention and control of the H9N2 influenza virus;(3) The application finds that a TRIM25 cell line with overexpression can promote replication of the H9N2 influenza virus, and TRIM25 or an expression promoter thereof can be used as a replication or production enhancer of the H9N2 influenza virus.

The application provides a gene and an application thereof CaCA2 The application belongs to the technical field of gene breeding and relates to application of a gene in pepper resistance to low-temperature and weak-light stress. CaCA2 The application discloses a gene and an application thereof CaCA2 The transient overexpression of the gene can slow down wilting of pepper plants, improve net photosynthetic rate of pepper leaves, expression amount of antioxidant enzyme genes, soluble sugar, free proline and mineral element content, and simultaneously reduce intercellular CO2 concentration and malondialdehyde content. CaCA2 After the VIGS technology is used to transiently silence the gene, the plant height, stem diameter, biomass and root morphological parameters of the pepper are reduced in varying degrees, and the gene causes stoma to close, and the overall fluorescence intensity, photosynthetic performance index and trace element content are reduced. CaCA2 The transient overexpression of the gene in the pepper can relieve wilting of the pepper plants and improve the resistance to low-temperature and weak-light stress, and the resistance is weakened after the gene is silenced.

The invention discloses a tea tree MYB transcription factor CsMYB330 gene and application, the nucleotide sequence of the MYB transcription factor CsMYB330 gene is shown as SEQ ID NO: 1, the expression of the gene participates in regulating the flavonol content in plants, and the instantaneous silencing of the CsMYB330 gene in the tea tree can significantly reduce the flavonol content in the tea tree by 24.5% and reduce the bitter taste of the finished tea.

The invention discloses siRNA (small interfering Ribonucleic Acid) of targeted bone morphogenetic protein 2, modified siRNA and application of the siRNA. According to the invention, siRNA drug design is carried out by taking the BMP2 gene as a target gene to obtain a series of siRNAs, siRNAs capable of effectively silencing the mRNA expression level of the BMP2 gene are screened from the siRNAs, and part of siRNA sequences can even silence the mRNA level of the BMP2 gene to about 25%. The siRNA sequence is further modified, wherein phosphorylation or phosphorylation analogue modification, skeleton modification, sugar ring modification and basic group modification are carried out on the 5'terminal of the sequence, or cholesterol molecule modification is carried out on the end group of the siRNA. The siRNA sequence is further subjected to cholesterol modification to obtain a human-mouse homologous sequence of the targeted BMP2 gene, and the in-vivo silencing activity of the BMP2 target spot is further researched in a mouse experimental model subsequently.

This invention discloses the application of the PhZPT2-5 gene in regulating anthocyanin synthesis in petunias, belonging to the field of plant genetic engineering technology. This invention discovers that the PhZPT2-5 gene is significantly correlated with anthocyanin synthesis in petunias. Experiments show that silencing the PhZPT2-5 gene reduces anthocyanin synthesis, while overexpressing the PhZPT2-5 gene promotes anthocyanin synthesis. By regulating the expression of the PhZPT2-5 gene in petunias, different colored petunias can be obtained, laying the foundation for breeding new petunia varieties.

The invention provides an RcERF98 gene and application of the RcERF98 gene to enhancement of gray mold resistance of Chinese roses, and belongs to the technical field of biological prevention and control. The nucleotide sequence of the RcERF98 gene is as shown in SEQ ID NO: 1. The RcERF98 gene is silent and overexpressed in petals which are good in growth state and free of botrytis cinerea infection of Chinese roses, and research results show that the petals treated by the silent RcERF98 gene are larger in scab area, higher in ion permeability, lower in hydrogen peroxide content and higher in ascorbic acid content, and the resistance of the petals of the Chinese roses to the botrytis cinerea is reduced; the petals treated by the overexpressed RcERF98 gene are smaller in scab area, lower in ion permeability, higher in hydrogen peroxide content and lower in ascorbic acid content, and the resistance of the Chinese rose petals to gray mold is enhanced.

The invention discloses application of an MdVQ22 gene in salt-tolerant genetic improvement of apples, and relates to the technical field of plant genetic engineering. The key salt stress response gene MdVQ22 is identified, and the salt tolerance of apple plants can be improved by silencing the gene. The analysis of phenotypes and related physiological indexes of the MdVQ22 transgenic line before and after salt treatment shows that compared with a non-transgenic line, MdVQ22 overexpression transgenic apple callus has obvious salt sensitivity, and compared with a contrast, a silent line of a silent MdVQ22 gene has an obvious salt tolerance advantage; therefore, the MdVQ22 gene is a potential salt-tolerant breeding gene and can be used for salt-tolerant genetic improvement of plants. The invention provides a new gene resource and technical path for cultivating salt-tolerant apple varieties, can promote the development of the apple planting industry in saline-alkali soil, and breaks through the limitation of land salinization on the high-quality development of the apple industry.

This invention provides a method for regulating nitrogen absorption and utilization in catalpa trees. CbuLBD9 This invention relates to genes and their applications, belonging to the field of forest tree genetic engineering breeding technology. CbuLBD9 The nucleotide sequence of the gene is shown in SEQ ID No. 1, and the amino acid sequence it encodes is shown in SEQ ID No. 2. This invention utilizes silencing... CbuLBD9 This gene significantly promotes the elongation and growth of the Catalpa tree root system and enhances the absorption and utilization of nitrogen by the plant roots. This invention provides an important candidate gene for genetic improvement related to efficient nitrogen utilization in Catalpa trees, and has broad application prospects in forest tree genetic engineering breeding and the development of Catalpa tree clones.

The application provides a LiCOMT gene for regulating synthesis of lycoris kingianus glycosides and application thereof, belongs to the technical field of plant genetic engineering, and the nucleotide sequence of the LiCOMT gene is shown as SEQ ID NO. 7, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 8; the application screens out a candidate gene LiCOMT significantly related to lycoris kingianus glycoside accumulation from lily bulb development period transcriptome data through weighted gene co-expression network analysis (WGCNA), and confirms that silencing the LiCOMT gene can reduce the content of lycoris kingianus glycoside A, lycoris kingianus glycoside B and lycoris kingianus glycoside F in lily bulbs by about 60%, 42% and 43% respectively by using a virus-induced gene silencing (VIGS) technique; and the application provides important gene resources and theoretical basis for lily secondary metabolism regulation and molecular breeding.

The invention discloses a poplar TCTP gene and application of the poplar TCTP gene in regulation and control of plant insect resistance. The TCTP gene capable of regulating and controlling the insect resistance of the plant is separated from the poplar, the TCTP gene is overexpressed or silenced in the poplar to obtain a transgenic poplar plant, and tests prove that the transgenic poplar plant with the overexpressed TCTP gene shows the character of improving the insect resistance; the transgenic poplar plant of the silent TCTP gene shows the property of insect resistance reduction, which indicates that the TCTP gene has the property of positively regulating the insect resistance of the plant. The method has an application prospect in the aspect of improving the insect resistance of plants.

The application discloses application of a reagent for inhibiting Igsf1 gene expression in preparation of a medicine for treating depression, and the depression symptom can be effectively improved by silencing Igsf1 gene expression or blocking the function of the Igsf1 gene by using an antibody, and the normal physiological state is not affected. Therefore, the Igsf1 can be used as a target in preparation and screening of the medicine for treating depression, and has an important clinical application prospect.

The invention relates to a method for increasing the content of ascorbic acid in kiwi fruits based on an AGPase gene. According to the method, the content of the ascorbic acid in the kiwi fruits is increased by silencing the AGPase gene. The nucleotide sequence of the AGPase gene in the method is as shown in SEQ ID NO: 1. On the basis of a large number of early-stage researches, the non-classical function of the AGPase gene in synthesis of the AsA of the kiwi fruit is clarified for the first time, the'starch metabolism regulation 'role of traditional cognition of the AGPase gene is broken through, the key function of the AGPase gene in synthesis of the AsA of the kiwi fruit is disclosed, the AsA content of the kiwi fruit can be directionally improved by regulating expression (such as silencing) of the AGPase gene, and the application of the AGPase gene in the synthesis of the AsA of the kiwi fruit is promoted. A new gene resource and a technical path are provided for kiwifruit molecular breeding, and a theoretical basis and a technical support are provided for breeding and creating a kiwifruit breeding material with high ascorbic acid content.

This invention belongs to the fields of microbiology and molecular biology, specifically relating to a homologous recombinase combination in myxobacteria and its application in gene editing. Specifically, this invention provides four pairs of homologous recombinase systems capable of efficiently mediating in vivo recombination in myxobacteria. Each pair includes a protein YqaJ with exonuclease function and a protein RecT with single-strand annealing function. This invention provides a method for myxobacterial gene editing: under the action of homologous recombinase pairs, mutant strains are obtained through positive screening using resistance tags. The resistance tags are then successfully removed through the combined action of the Cre / loxP system and the reverse screening gene galK. This method achieves a gene editing efficiency of up to 100%, has a short cycle time, requires no purification, and can be used for iterative deletion of multiple genes to achieve genome simplification in myxobacteria, or for inserting functional promoters and other elements to activate silent gene clusters. This method is of great significance for the bioexploration and functional genomics research of myxobacteria.