188 results about "Silent gene" patented technology

The invention discloses an application of an RcWRKY31 gene in enhancing the resistance of Chinese rose to gray mold. The nucleotide sequence of the RcWRKY31 gene is as shown in SEQ ID NO. 1. The RcWRKY31 gene is silenced and overexpressed in petals which are good in Chinese rose growth state and free of botrytis cinerea infection, and research results show that the petals treated by the silent RcWRKY31 gene are larger in scab area and lower in resistance to botrytis cinerea; the resistance of petals treated by overexpression of the RcWRKY31 gene to gray mold is enhanced.

The invention discloses application of a Chinese rose gene RhMYB1 in regulation of Chinese rose axillary bud germination. Relates to the technical field of molecular biology, and provides application of a product for inhibiting expression of an RhMYB1 gene or transcriptional translation protein of the RhMYB1 gene in regulation of axillary bud germination of Chinese roses, and a nucleotide sequence of the RhMYB1 gene is shown as SEQ ID NO.1. According to expression results of the RhMYB1 gene in buds at different sites of Chinese roses, it is found that the expression quantity of the RhMYB1 gene in upper buds (namely active buds) is high. The RhMYB1 gene in the axillary bud is instantaneously silenced, the silent RhMYB1 gene is found to significantly inhibit the germination and growth of the axillary bud, and the overexpression RhMYB1 can promote the growth of the axillary bud.

The invention belongs to the technical field of gene engineering, and discloses a wheat susceptibility related gene, a protein and application. The wheat susceptibility related gene is a TaBRG10 gene and is derived from a wheat variety water source 11, the nucleotide sequence of the gene is as shown in SEQ ID NO: 1, and the amino acid sequence of protein encoded by the TaBRG10 gene is as shown in SEQ ID NO: 2. The TaBRG10 gene is induced to express by stripe rust, and under the infection of stripe rust, the infection surface of a TaBRG10 gene silent plant infected by stripe rust is positively and obviously lower than that of a control plant, which indicates that the silent TaBRG10 gene improves the resistance of the plant to wheat stripe rust. The invention provides a novel germplasm material for the cultivation of the stripe rust resistant wheat variety.

The present disclosure provides Crispr / cas9-based repressors for silencing gene targets in vivo and methods of use.

The invention discloses an application of a tomato Solyc04g064880 gene in regulation and control of ToBRFV resistance of a plant, a Solyc04g064880 gene silencing vector pTRV2 is constructed: S14880, agrobacterium tumefaciens is transformed, positive bacteria obtained by screening are inoculated into a target plant to obtain a Solyc04g064880 gene silencing strain, and compared with a contrast, the Solyc04g064880 gene silencing strain has the advantages that the ToBRFV accumulation amount in the plant is reduced, and the ToBRFV resistance of the plant can be regulated and controlled. Therefore, the silent Solyc04g064880 gene can improve the resistance of the plant to the ToBRFV, and a technical support is provided for the breeding of a ToBRFV resistant variety of the plant.

The invention discloses an effect protein E56, a coding gene and application of the effect protein E56 to improvement of southern rust resistance of corn, and belongs to the technical field of agricultural biology. The amino acid sequence of the effector protein E56 is as shown in SEQ ID NO. 1. A reverse genetics method is utilized to analyze the Puccinia polystachys effect protein E56 gene, and the E56 gene is up-regulated in expression in the Puccinia polystachys infection process. The E56 gene is silenced by adopting a host-mediated gene silencing technology, and the toxic function of the E56 gene in the infection process of the southern rust disease is determined. A specific fragment of the E56 gene is cloned to an RNAi interference vector, a corn immature embryo is transformed by using an agrobacterium tumefaciens-mediated transgenic technology, and an obtained transgenic corn plant shows resistance to the corn southern rust disease. The E56-RNAi transgenic plant shows resistance to puccinia polypoda, so that the effect protein E56 can be used for creating a new strain for resisting the southern rust disease.

The invention relates to the technical field of plant genetic engineering, and provides a receptor-like kinase gene for improving Orobanche coerulescens parasitism resistance of sunflower and application of the receptor-like kinase gene. The invention discovers an amino acid sequence of the sunflower receptor kinase and a nucleotide sequence of the coding gene HaCERK1, and provides a target sequence for silencing the HaCERK1 gene, a virus-mediated gene silencing system and a silencing method based on the amino acid sequence, so that the expression level of the sunflower receptor kinase HaCERK1 gene can be effectively inhibited, and the expression level of the sunflower receptor kinase HaCERK1 gene can be effectively inhibited. And a virus-mediated gene silencing system can be systematically diffused in sunflower and sunflower orobanche tissues, so that the expression of the HaCERK1 gene is effectively interfered. According to the virus-mediated gene silencing system and method provided by the invention, the sunflower gene function research efficiency is remarkably improved, and the time and the cost are greatly reduced.

The invention belongs to the technical field of gene engineering, and particularly relates to application of a DcTH gene in prevention and control of diaphorina citri, and the nucleotide sequence of the DcTH gene is shown as SEQ ID NO.1. The tyrosine hydroxylase DcTH gene is accurately identified and screened by taking diaphorina citri as a research object. Further, dsRNA is successfully synthesized by designing a specific primer, and an RNA interference technology is applied to the expression of the DcTH gene. Researches find that after the DcTH gene is silenced, the key physiological process of ecdysis of the diaphorina citri is interfered, the malformation rate and the death rate are remarkably increased, and finally effective prevention and control of the diaphorina citri are achieved. The DcTH gene provided by the invention can further provide a brand-new molecular target and theoretical support for development of a diaphorina citri prevention and control technical means based on RNAi (Ribonucleic Acid Interfere).

The invention relates to a cotton leaflet character regulation gene and an encoded protein and application thereof, and relates to the technical field of plant genetic engineering, the nucleotide sequence of the gene is shown as SEQ ID NO.1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO.2. According to the invention, a gene capable of regulating and controlling the leaf rolling character of cotton is obtained by cloning from the goblet oilless cotton and is named as a Ghcup gene, and silencing research is carried out on the Ghcup gene in upland cotton goblet oilless cotton by constructing a VIGS vector of the Ghcup gene of the cotton, so that the Ghcup gene can be used for regulating and controlling the leaf rolling character of the cotton. A cotton Ghcup gene overexpression vector is constructed, transgenic overexpression is carried out in normal broad-leaf upland cotton Xinluzhong 75, and results show that the curly phenotype of an oilless goblet-leaf cotton leaf of a silent Ghcup gene disappears, the overexpression of the gene causes the upward curly shape of the Xinluzhong 75 cotton leaf to be similar to a cup shape, and the cotton Ghcup gene overexpression vector is used for transgenosis overexpression in the normal broad-leaf upland cotton Xinluzhong 75. Therefore, the Ghcup gene has great application potential in the aspects of improving the leaf shape and the plant canopy structure of the cotton, improving the photosynthetic efficiency of the plant and the like.

The invention relates to a strong promoter P7 suitable for streptomyces and application thereof, and relates to the field of genetic engineering and microbial metabolism engineering. The nucleotide sequence of the strong promoter P7 is as shown in SEQ ID No.1, and the strong promoter comprises a plasmid vector of the strong promoter; a host cell comprising the plasmid vector; the invention also discloses application of the strong promoter, the plasmid vector and the host cell in starting expression of a target gene. Compared with the prior art, the characterization of the strong promoter P7 provides an effective tool element for streptomyces strong promoter engineering and high-efficiency gene expression, and has important significance on streptomyces silent gene characterization, high-efficiency gene expression, metabolite synthesis, metabolic pathway reconstruction and the like. The strong promoter can be applied to common streptomyces type strains, and has important significance on high yield of important proteins including enzymes and important metabolites from actinomycetes.

The invention provides a jasmine VIGS silencing system as well as a construction method and application thereof, and belongs to the technical field of plant genetic engineering. The method comprises the following steps: firstly, providing a specific nucleotide fragment for silencing the arabian jasmine flower PDS gene, and inserting the specific nucleotide fragment into a pTRV2 vector through homologous recombination by taking a tobacco embrittlement virus (TRV) as the vector, so as to construct a silencing vector pTRV2-JsPDS; a vector pTRV1, a vector pTRV2 and a silence vector pTRV2-JsPDS are respectively transferred into agrobacterium to obtain a VIGS silence system of the jasmine flower PDS gene, the silence system is adopted to infect a jasmine flower plant, experiments prove that the silence system can induce silence of the jasmine flower JsPDS gene, the total chlorophyll content in jasmine flower leaves after gene silence is reduced by 34.98-63.79%, and the total chlorophyll content in the jasmine flower leaves after gene silence is reduced by 34.98-63.79%. And the relative expression quantity of the endogenous JsPDS gene is reduced by 56.38%-85.79%. The method has the advantages of being easy and convenient to operate, short in period, high in silencing efficiency, free of complete gene sequence information and genetic transformation systems and the like, and an efficient and reliable technical means is provided for arabian jasmine flower gene function research.

The invention discloses an application of a ClBZR1L gene in enhancing the cold resistance of watermelons by promoting proline accumulation. The invention belongs to the technical field of plant genetic engineering. The CDS sequence of the ClBZR1L gene is as shown in SEQ ID NO. 1. Experiments show that when a recombinant vector for constructing the silent ClBZR1L gene is transferred into a watermelon plant, the proline content of the silent plant under cold stress treatment is remarkably reduced compared with that of a control group, and the cold resistance is reduced. A recombinant vector for constructing the over-expression ClBZR1L gene is transferred into a watermelon plant, the proline accumulation amount of the over-expression plant under cold stress treatment is remarkably increased, the cold resistance is enhanced, and a gene resource is provided for watermelon cold-resistant molecular breeding. Besides, the invention also discloses direct combination of the ClBZR1L gene and a proline synthesis rate-limiting enzyme coding gene ClP5CS1 gene promoter, and the expression of the coding gene is up-regulated, so that synthesis of proline under cold stress is promoted, and a theoretical basis is provided for in-depth study on cold-resistant gene pathways.

The invention relates to an shRNA (short hairpin Ribonucleic Acid) interference sequence of a targeted silence PCSK9 gene and a construction method and lipid-lowering application of a recombinant adeno-associated virus vector of the shRNA interference sequence. Hyperlipidaemia is a metabolic disease characterized by abnormal rising of cholesterol and triglyceride levels in blood, and the design of lipid-lowering drugs is the focus of attention to improvement of hyperlipidaemia. Proprotein convertase subtilisin / kexin type 9 (PCSK9) can be combined with a low-density lipoprotein receptor (LDL-R) and degrade the LDL-R, so that accumulation of LDL-C in blood is further promoted, and hyperlipidemia is caused. Aiming at the key target PCSK9, a specific shRNA interference sequence is designed, and a recombinant adeno-associated virus vector (rAAV) carrying the sequence is constructed by an enzyme digestion-connection method. In-vitro experiments prove that the vector can remarkably reduce the expression level of PCSK9 protein, so that the cyclic utilization of a low-density lipoprotein receptor (LDL-R) is promoted, and the concentration of low-density lipoprotein cholesterol (LDL-C) in plasma is reduced. The rAAV vector provided by the invention has the characteristics of low production cost, high transfection efficiency, lasting action time and the like, and provides a new thought for gene therapy of hyperlipidemia.

This invention relates to the fields of molecular biology and biomedicine, and discloses siRNA for the expression of the *Schistosoma japonicum* Hsc20 gene and its applications. The siRNA contains a nucleotide sequence that hybridizes to a target sequence of the *Schistosoma japonicum* Hsc20 gene, wherein the target sequence is selected from the sequences shown in SEQ ID NO. 1-SEQ ID NO. 4. The siRNA specifically inhibiting the expression of the *Schistosoma japonicum* Hsc20 gene provided by this invention can be used to interfere with the transcription and expression of the *Schistosoma japonicum* Hsc20 gene and the growth and development of *Schistosoma japonicum*. In vitro experiments have confirmed that the siRNA provided by this invention can efficiently silence the *SjHsc20* gene; and in vivo RNA interference experiments in mice show that this siRNA can induce 50.62% (…) P The insect reduction rate was <0.05% and 44.29% ( P A liver oocyte reduction rate of <0.01% is suitable for preparing drugs to treat schistosomiasis.

A method for modifying a gene encoding or processed to a non-coding RNA molecule having no RNA silencing activity in a eukaryotic cell is disclosed, with the proviso that the eukaryotic cell is not a plant cell. The method comprises introducing a DNA editor into the eukaryotic cell, the DNA editor conferring a silence specificity of the non-coding RNA molecule for a target RNA of interest. Also disclosed is a method of modifying a gene for encoding or being processed into an RNA silencing molecule for a target RNA in a eukaryotic cell. Also disclosed are methods of preventing and treating various diseases, methods of inducing apoptosis, and methods of producing an eukaryotic non-human organism.

The application discloses a kiwi bacterial canker disease susceptible gene AcADC1 and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the AcADC1 gene is shown in SEQ ID NO:1, and the arginine decarboxylase (ADC) synthesized by the gene is encoded, and the amino acid sequence is shown in SEQ ID NO:2, and the arginine (Arg) is responsible for catalyzing the formation of putrescine. The resistance of the kiwi plant with the silenced AcADC1 gene to the bacterial canker disease is improved, the resistance of the kiwi plant with the overexpressed AcADC1 gene to the bacterial canker disease is reduced, the AcADC1 gene plays a negative regulation role in the immune response of the kiwi to the bacterial canker disease, the gene editing technology can be used for editing the AcADC1 gene, and a theoretical basis and gene reserve are provided for kiwi disease-resistant breeding.

Provided herein are compositions, systems, and methods for the creation, identification, and characterization of effector domains for activating and silencing gene expression. In particular, a high-throughput system for discovering and characterizing effector domains is provided.

The invention relates to the technical field of biology, in particular to application of an OsY37 gene to regulation and control of stigma vitality of a rice sterile line. According to the present invention, the rice sterile line stigma vitality is improved by knocking out or silencing the OsY37 gene, or the rice sterile line stigma vitality is reduced by up-regulating the expression level of the OsY37 gene, the DNA sequence of the OsY37 gene is represented by SEQ ID NO.1, and the knockout or OsY37 gene can effectively improve the rice sterile line stigma vitality so as to effectively prolong the pollination period of the rice stigma and improve the hybrid rice seed yield.

The invention discloses a method for synergistically improving the lycopene content and the sugar-acid ratio in tomato fruits, belongs to the technical field of biology, and particularly relates to a method for knocking out or silencing an INVINH1 gene to synergistically improve the lycopene content and the sugar-acid ratio in the tomato fruits, and a nucleotide sequence of a protein coding region of the INVINH1 gene is shown as SEQ ID NO.1. The invention further discloses a method for synergistically improving the lycopene content and the sugar-acid ratio in the tomato fruits. A carrier for knocking out or silencing the INVINH1 gene is constructed by utilizing a CRISPR / Cas9 gene editing technology, the carrier is transferred into tomato cotyledons through agrobacterium tumefaciens mediation, INVINH1 gene knockout homozygous strains invinh1-1 and invinh1-2 are obtained through screening by utilizing a tissue culture technology, the lycopene content and saccharic acid in tomato fruits of a mutant plant are remarkably improved compared with those of wild plants, and the gene knockout or silencing method is suitable for industrial production. The invention provides an important gene resource for cultivating tomato varieties for synergistically improving lycopene and sugar-acid ratio.

The invention relates to the technical field of biology, in particular to application of an RhUNE10 gene to regulation and control of root cancer resistance of Chinese roses and a regulation and control method. The nucleotide sequence of the RhUNE10 gene is as shown in SEQ ID NO. 1. The application mode is that the RhUNE10 gene is silenced and knocked out, or the expression of the protein coded by the RhUNE10 gene is inhibited; and the amino acid sequence of the protein coded by the RhUNE10 gene is as shown in SEQ ID NO. 3. After the RhUNE10 gene is silenced, the resistance of the Chinese rose to agrobacterium tumefaciens is remarkably reduced, which indicates that the RhUNE10 gene has important biological functions, is beneficial to promoting Chinese rose functional genomics research, gene editing breeding and transgenic breeding research, excavates and cultivates new varieties of high-resistance Chinese roses, and has wide application prospects. And a certain theoretical basis and key gene resources are provided for comprehensive prevention and treatment of root cancer and cultivation of a new variety of Chinese rose.

The present application relates to a nucleic acid molecule inhibiting ANGPTL3 gene expression. The nucleic acid molecule contains or consists of substantially complementary sense and antisense sequences, wherein the sense sequence and / or antisense sequence has a nucleotide length of 14-30 nt. The nucleic acid molecule can better specifically silence an ANGPTL3 gene.

The invention belongs to the field of biological medicine, and particularly relates to a YBX3 gene, a YBX3 gene inhibitor and application of the YBX3 gene inhibitor. The invention discloses application of the YBX3 gene as a biomarker in preparation of a product for detecting cardiac fibrosis. The nucleotide sequence of the YBX3 gene is as shown in SEQ ID NO. 1. The invention discloses application of a YBX3 gene inhibitor in preparation of drugs for preventing and / or treating heart diseases. The YBX3 gene inhibitor is shRNA (short hairpin ribonucleic acid) and has a nucleotide sequence as shown in SEQ ID NO. 3. When myocardial infarction occurs, mRNA and protein level expression activity of the YBX3 gene is remarkably improved and is related to fibroblast activation, and the silent YBX3 gene can remarkably inhibit fibroblast activation. The existing myocardial infarction injury medicine has a limited treatment effect, so that the YBX3 can improve the clinical prognosis of myocardial infarction injury by regulating and controlling the activation of cell fibrosis.

This invention discloses NtBRL2 The application of genes in regulating potassium content in plants belongs to the field of plant genetic engineering technology. This invention provides a gene that can effectively regulate potassium content in plant leaves, a feature of existing technologies. NtBRL2 This invention first discovered through Real-time PCR. NtBRL2 The gene was expressed at the highest level in tobacco roots; then, a silenced gene was constructed using virus-induced gene silencing (VIGS) technology. NtBRL2 The VIGS vector of the gene was transformed into Nicotiana benthamiana, and repression was successfully achieved. NtBRL2 Silent plants, obtained by silencing gene expression, exhibit a specific phenotype with significantly lower potassium ion content compared to control plants, indicating that silencing this gene can significantly reduce potassium ion content in plants. This invention lays the foundation for elucidating the biological regulation mechanism of potassium ions and provides precise regulatory sites for the regulation of potassium ion content in plants.

The invention discloses application of expression of a targeted silence TdSb-sbd gene in increasing the egg laying amount of female trichogramma dendrolimus, and belongs to the technical field of biological prevention and control. The invention discovers that the SPS protein (coding gene TdSb-sbd) of trichogramma dendrolimus can interact with the juvenile hormone degradation key enzyme JHEH1 protein. After the TdSb-sbd gene is silent in a targeted manner, the expression level of the gene can be reduced, and the expression of an interaction protein coding gene Tdjheh1 of the gene is reduced, so that the degradation pathway of juvenile hormone (JH) is inhibited, and the titer of juvenile hormone in trichogramma is improved. The high juvenile hormone level can promote ovarian development and ovum generation of trichogramma, and finally the egg laying amount of female bees is remarkably increased. The trichogramma reproductive capacity is directly regulated and controlled through a molecular biology means, and a new technical scheme is provided for improving the trichogramma dendrolimus reproductive capacity and the field pest control efficiency on a large scale.

This invention discloses the application of platelet-reactive protein-4 (THBS4) in the diagnosis and treatment of endometriosis. Through bioinformatics integration analysis, clinical sample validation, and in vitro and in vivo experiments, this invention confirms that THBS4 is significantly highly expressed in both ectopic lesions and peripheral blood of patients with endometriosis, and its expression level is positively correlated with disease severity. The area under the receiver operating characteristic (AUC) curve for THBS4 alone in diagnosing endometriosis is 0.930, significantly superior to cancer antigen 125 (CA125); the AUC for the combined diagnosis of THBS4 and CA125 reaches 0.968. Simultaneously, silencing the THBS4 gene effectively inhibits the proliferation, migration, and invasion of human endometrial stromal cells, and significantly reduces the volume and fibrosis area of ​​ectopic lesions in a mouse model of endometriosis. This invention provides a highly sensitive and specific new biomarker for the non-invasive or minimally invasive early diagnosis of endometriosis, and provides new targets and candidate drugs for non-hormone-dependent targeted therapy.

The invention relates to male bee sperms in which the target region having the nucleotide sequence SEQ ID NO:1 on the JHAMT (Gene ID: 724216) gene is silenced by the forward primer having the nucleotide sequence SEQ ID NO:2 by means of CRISPR-Cas9 technology, and to the insemination of young queen bees with these sperms via artificial insemination. With the repetition of the same procedures, two generation queen bees were bred, and mutant bees were produced, silencing the Jhamt gene. By means of silencing this gene, bees resistant to Varroa destructor parasite are obtained.

The invention discloses a cotton verticillium wilt resistance related gene GhACA12 and application thereof in disease-resistant breeding, and belongs to the technical field of agricultural biology. Through whole genome association analysis (GWAS), an SNP site D11: 57218252 related to verticillium wilt resistance is found in a cotton D11 chromosome region, and a GhACA12 gene is screened out as a candidate disease-resistant gene. Researches show that high expression of GhACA12 is related to susceptible characters, and low expression of GhACA12 is related to disease resistance characters. The GhACA12 gene is silenced through a VIGS technology, and the disease resistance of the cotton is remarkably enhanced. Specific primers are designed for qRT-PCR detection, and disease-resistant materials are screened in an auxiliary mode. The invention provides effective technical support for cotton verticillium wilt resistant molecular breeding, and has important application value.

The application is suitable for the field of biological medicine technology, and provides a siRNA-PSMA conjugate targeting a LEPR gene, a preparation method and application thereof. The siRNA-PSMA conjugate realizes specific targeted delivery of CRPC cells through a PSMA inhibitor target head, effectively reduces off-target toxicity, combines with a high-interference-efficiency M3 modified LEPR siRNA screened, can efficiently silence LEPR gene expression, block a leptin-LEPR signal pathway, and then inhibit CRPC cell proliferation, invasion and drug resistance related biological functions; the preparation process is stable and controllable, the purity of the finished product after purification can reach more than 95%, meets the quality requirements of drug development, and the activity verification method has strong specificity and reliable results, fills the blank of CRPC targeted therapy drugs in the prior art, provides a new precise targeting strategy for CRPC treatment, and has important clinical application value and broad conversion prospect.

The invention discloses application of a GhGDSL102 gene in regulating and controlling the oil content of a plant, and belongs to the technical field of gene engineering. The GhGDSL102 gene is over-expressed in arabidopsis thaliana, the oil content of arabidopsis thaliana seeds is obviously reduced by 13.67%, and oleic acid C18: 1 is obviously reduced by 7.7%. After the GhGDSL102 gene is silenced, the oil content and oleic acid content of the cotton seeds are obviously increased by 9.99% and 7.57%. Meanwhile, three transgenic positive strains are obtained through overexpression of cotton, it is found through measurement of the oil content of cotton seeds that the oil content of the three overexpression strains is remarkably reduced by 11.70%, 9.14% and 19.25% respectively compared with that of a control group, and the oleic acid content is reduced by 5.54%, 7.09% and 6.29% respectively; the cottonseed oil content of the obtained homozygous edited strain is remarkably improved by 19.4%, and the GhGDSL102 is proved to be a gene for negatively regulating the cottonseed oil content.

The invention relates to application of a TM4SF1 gene and an inhibitor of the TM4SF1 gene in detection of PVR or preparation of a medicine for treating the PVR, and belongs to the field of biological medicine. PVRMs samples are sequenced, one fibroblast subtype is found to be derived from differentiation of RPE cells through cell subset clustering analysis and quasi-timing analysis, and the TM4SF1 gene participates in regulation and control of the process. The method comprises the following steps: constructing a short interference oligonucleotide capable of silencing a TM4SF1 gene to obtain a TM4SF1 silencing RNA (Ribonucleic Acid); through a Transwell migration experiment, it is found that after the TM4SF1 gene is silenced, fibrosis change caused by TGF-beta 1 can be inhibited; immunohistochemistry and immunocytochemistry show that after the TM4SF1 is silenced, the fibrosis change caused by the TGF-beta 1 can be inhibited through the (TM4SF1-siRNA). The method provided by the invention shows that the TM4SF1 gene expression level can be used as a basis for detecting the proliferative vitretinopathy; the method provided by the invention also shows that the effect of treating proliferative vitretinopathy can be achieved by inhibiting the expression of the TM4SF1 gene.