Gene drug co-carrier system using cation liposome to establish gold nano-spherical shell and preparation method

A gold nanosphere shell and cation technology, applied in gene therapy, drug combination, pharmaceutical formulation, etc., can solve the problems of difficult removal of impurities, gene instability, gene decomposition, etc.
CN102805873AInactive Publication Date: 2012-12-05TIANJIN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TIANJIN UNIV
Publication Date
2012-12-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a gene drug co-carrier system using cation liposome to establish a gold nano-spherical shell and a preparation method. The method comprises: using phosphatides such as DPPC, MPPC and DPPE-PEG and the derivatives thereof to form the cation liposome via membrane extrusion; carrying the hydrophilic drug hydrochloric acid adriacin into the liposome in the forming process; forming the drug-carried cation liposome; adding chlorauric acid solution into cation liposome solution; adsorbing on the surface of the cation liposome; adding ascorbic acid solution for reduction; forming the gold nano-spherical shell structure; reducing sulfhydryl out of the single chain of green fluorescent protein silent gene with a disulfide bond, grafting onto the surface of the gold nano-spherical shell; and forming the gene drug co-carrier system. The gold nano-spherical shell has low toxicity and unique optical properties. The gene drug co-carrier system using cation liposome to establish the gold nano-spherical shell can provide carrier for the co-treatment of drugs and genes, can select light to illuminate the gold nano-spherical shell, and utilize light-heat conversion property to realize the co-release of the drugs and genes.
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Description

technical field

[0001] The invention relates to the formation of a gold nano-shell structure using a cationic liposome as a template, and using the gold nano-shell structure as a carrier to construct a drug and gene co-carrying system. Because siRNA etc. Background technique

[0002] Cationic liposomes are widely used in drug carriers and gene carriers, but liposomes are metastable structures, not stable enough in body fluids, and have a relatively short circulation time in the body. Cationic liposomes can be modified to improve their stability.

[0003] Gold nanomaterials have relatively unique physical and chemical properties. The gold nanosphere shell structure has an absorption peak in the near-infrared region due to the surface plasmon resonance effect. Researchers have used liposomes as templates to deposit gold on the surface to form hollow nanosphere structures, and used liposomes to carry anticancer drugs such as doxorubicin hydrochloride, and irradiated gold nano...

Claims

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