10results about How to "The detection method is simple" patented technology

This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.

This invention relates to a digital radiographic inspection method and apparatus for wind turbine blades, belonging to the field of wind turbine blade inspection technology. The method includes: placing and fixing the turbine blade on a blade fixture; mounting the X-ray machine on the X-ray machine fixture; defining a detection grid in the inspection area of ​​the turbine blade; tightly attaching and fixing the X-ray flat panel detector to the turbine blade; and sequentially inspecting each grid of the turbine blade according to the detection grid. This invention, through the combination of the blade fixture, the X-ray machine fixture, the X-ray machine, and the X-ray flat panel detector, constitutes a digital radiographic inspection apparatus for turbine blades. This apparatus enables the detection of defects in wind turbine blades. The inspection method of this invention is simple and rapid; the inspection images obtained by this invention can clearly and accurately identify defects in wind turbine blades, with a defect detection rate of 100%.

This utility model relates to a novel high-voltage cable coupler, comprising a coupler plug, a coupler socket, a measurement module, and an energy harvesting module. Windows and / or aviation sockets are provided on both the coupler plug and the coupler socket. The measurement module and the energy harvesting module are encapsulated within the entire cable coupler. The high-voltage cable coupler in this design has windows for wireless communication, optical detection, and image recognition functions. This design detects the ambient temperature and humidity, arc light, contact temperature, electrical quantities, and internal temperature of the high-voltage cable coupler, providing support for intelligent monitoring and status judgment, fault detection, and location of the high-voltage cable coupler. The energy harvesting module in this design features adaptive energy management, adapting to various complex environments, eliminating the need for an external power source, freeing it from energy constraints, improving equipment autonomy, reliability, and sustainability, and reducing maintenance costs.

The present application provides a SNP molecular marker related to wet damage tolerance of Brassica napus and application thereof, wherein the sequence of the SNP molecular marker is shown as SEQ ID No. 1, and an A / G base mutation exists at 11872958 of the sequence shown as SEQ ID No. 1. The SNP molecular marker provided by the present application is based on a competitive allele-specific PCR marker detection method, and is used for detection and identification of the wet damage tolerance trait of Brassica napus. The detection method is simple in operation, low in cost, accurate in detection result, good in repeatability and stability, and different detection laboratories and different data results can be compared and verified with each other, and the data has universal comparability.

A norfloxacin sensor and its preparation and detection methods are disclosed, relating to the field of electrochemical sensor technology. The preparation method includes: S1 preparing a CTS / Ag-NiWO4 composite material; S2 preparing a multi-walled carbon nanotube solution; S3 polishing and activating the surface of a base electrode; S4 preparing a first solid film on the surface of the base electrode using the multi-walled carbon nanotube solution; and S5 preparing a second solid film on the surface of the first solid film using the CTS / Ag-NiWO4 composite material. This invention utilizes multi-walled carbon nanotubes, Ag-NiWO4, and chitosan to construct the sensor interface, enabling quantitative detection of norfloxacin concentration. It not only exhibits strong adsorption, catalytic, and conductivity capabilities, but also boasts a simple and easy-to-implement preparation and detection method, high sensitivity, and a low detection limit.

This utility model relates to the field of testing platform technology, specifically to a testing platform for the perpendicularity of a cylinder. It includes a fixed base with two first rotating slots at its top. A first rotating shaft and a second rotating shaft are rotatably connected to the two first rotating slots, respectively. A storage tray is fixedly connected to one end of each first rotating shaft extending through the first rotating slots to the outside of the fixed base. A magnet is fixedly connected to the inner cavity of the upper end of the storage tray. A metal cylinder is placed in the middle of the storage tray. A threaded sleeve is fixedly connected to one end of the second rotating shaft extending through the first rotating slots to the outside of the fixed base. A threaded rod is threadedly connected to the inside of the threaded sleeve. Compared to existing technologies, this application offers a simpler testing method, is less affected by the end face area of ​​the metal cylinder, improves the accuracy of detecting the perpendicularity of the metal cylinder's end face, and allows the dial indicator to measure metal cylinders with various height and diameter values, thus enhancing the practicality of the testing platform.

The present application relates to a kind of C-MYC and FGFR1 gene fluorescence in situ hybridization combined detection probe and its application, belong to biological medicine technical field.The present application provides a kind of probe and probe composition for tumor diagnosis and prognosis evaluation, including the first probe for detecting C-MYC gene, and the second probe for detecting FGFR1 gene, the first probe and the second probe are labeled with the first fluorescein and the second fluorescein that can produce different colors respectively;By the present application, a kind of fluorescence in situ hybridization combined detection method and its kit for simultaneously detecting C-MYC and FGFR1 gene variation are provided;Wide application range can be used in various tissue and cell samples needing simultaneously detecting C-MYC and FGFR1 gene state, one detection, two results, meet the goal of clinical test quality improvement and efficiency improvement;It has quite extensive application prospect in the prognosis of various cancers.

The application of the biomarker combination in the preparation of a drug for fulminant myocarditis belongs to the field of drugs. The application is characterized in that the biomarker combination comprises Siglec-5; the biomarker combination is further selected from the group consisting of sST2, PAI-1, Siglec-5, CD163, CD40, P-Cadherin, CD14, CTLA4. The application finds that the expression of the biomarker in the plasma of a patient with fulminant myocarditis is increased, and confirms the role of the biomarker as a drug target in relieving and / or improving fulminant myocarditis. The level of the biomarker can be controlled by a drug, the diagnosis and subsequent treatment of the disease can be more intensified, and the biomarker has great application value in clinical practice.

The application provides a detection method for water content in electronic fluorination liquid, which uses external extraction method to pretreat the sample, adopts Karl Fischer coulometric method to measure the water content in the electronic fluorination liquid, and the Karl Fischer reagent is composed of 50% ethanol, 10% diethanolamine, 10% imidazole, 10% methanol, 5% sulfur dioxide, 5% hydroiodic acid, 5% hydrogen bromide, 5% iodine in mass fraction, and the sample needs to be pretreated by the external extraction method before the water content is detected; the water content m1 of the measured sample, the water content m2 of the extraction solvent, the weight x1 of the solvent and the weight x2 of the sample are recorded respectively, correction is conducted by using a correction formula, and finally the water content in the electronic fluorination liquid is determined.