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STON1 gene molecular marker primer related to chicken double-yolk egg traits and application of STON1 gene molecular marker primer
ActiveCN119320833AEfficient identificationThe detection method is simpleMicrobiological testing/measurementFood processingYolkAnimal science
The invention relates to an STON1 gene molecular marker primer related to chicken double-yolk egg traits and application of the STON1 gene molecular marker primer, and belongs to the technical field of biology. The STON1 gene molecular marker is a 7867409th basic group of a chicken reference genome GRCg7b version chromosome 3, the basic group is mutated into A or G, and the genotypes are A / A, A / G and G / G. The double-yolk egg ratio of the G / G genotype chicken is higher than that of the A / G and A / A genotype individuals, and the double-yolk egg ratio of the A / G genotype chicken is higher than that of the A / A genotype individuals. A forward and reverse primer pair is used for amplifying a fragment containing the molecular marker site, Sanger sequencing is carried out on an amplification product, the genotype of an individual is rapidly and accurately identified to serve as a genetic marker for chicken breeding, and the chicken with the higher proportion of double-yolk eggs is bred; the double-yolk egg traits can be efficiently and accurately detected, the breeding accuracy and breeding efficiency of the chicken double-yolk egg proportion are improved, and the method has important value for chicken breeding and production.
Owner:NANJING AGRICULTURAL UNIVERSITY
SNP molecular marker related to IMF character of Ningxiang pig and application of SNP molecular marker
ActiveCN119265310AThe detection method is simpleQuick checkMicrobiological testing/measurementFood processingPork meatZoology
The invention discloses an SNP molecular marker related to an IMF character of a Ningxiang pig and application of the SNP molecular marker. The SNP molecular marker comprises at least one of an SNP1 site, an SNP2 site, an SNP3 site, an SNP4 site, an SNP5 site and an SNP6 site. According to the scheme, the SNP molecular marker related to the IMF character is provided, Ningxiang pig breeds with different IMF contents can be efficiently and accurately identified and screened out, and the SNP molecular marker has the advantages of being rapid, accurate and low in detection cost, can be used for detection and application of different Ningxiang pig breeds and has wide application prospects. And an indispensable important tool is provided for breeding Ningxiang pig breeds with high IMF content and excellent meat quality and improving the quality of Ningxiang pork.
Owner:INSTITUTE OF SUBTROPICAL AGRICULTURE CHINESE ACADEMY OF SCIENCES
Pipe size detection mechanism for plastic pipe discharging
ActiveCN222353099UThe detection method is simpleImprove detection efficiencyPlastic recyclingWork holdersPipe waterStructural engineering
The utility model discloses a pipe size detection mechanism for plastic pipe discharge, which relates to the technical field of pipe detection equipment and comprises a pipeline clamping mechanism, a pipe placing frame, a pipeline outer diameter detection mechanism and a pipeline inner diameter detection mechanism, and the pipeline outer diameter detection mechanism and the pipeline inner diameter detection mechanism are fixed at two ends of the pipe placing frame. The pipeline clamping mechanism is fixed to a power trolley of the track power trolley, a linear track of the track power trolley is located in front of the pipe placing frame, the pipeline clamping mechanism clamps the end of a pipeline, and the pipeline is horizontally placed on the pipe placing frame. According to the utility model, the pipeline clamping mechanism, the pipe placing frame and the track power trolley are combined with the pipeline outer diameter detection mechanism and the pipeline inner diameter detection mechanism, so that the wall thickness and the outer diameter of the whole pipeline can be automatically detected, the detection mode is simple, the detection efficiency is high, and the uniformity of the pipeline size can be detected; the positioning accuracy ensures that the to-be-detected pipeline does not deform and bend, the measurement precision is improved, and the detection effect is good.
Owner:HUBEI KINGBULL PIPE IND
Self-adaptive alternating-current series arc fault detection system based on cloud-edge collaboration
PendingCN119518584AHigh precisionImprove generalization abilityTesting dielectric strengthBiological modelsFrequency couplingSelf adaptive
The invention relates to a self-adaptive alternating-current series arc fault detection system based on cloud edge cooperation, and belongs to the field of arc fault detection. The system comprises an arc fault protector which carries a lightweight arc fault detection model based on depth separable convolution and a reverse residual block, collects a high-frequency coupling signal of a line in real time, and transmits the high-frequency coupling signal to a cloud server; the cloud server receives and screens misjudgment data and unknown load combination fault data uploaded by the arc fault protector in the operation process, and updates an arc fault detection model on the arc fault protector through data enhancement and transfer learning. According to the method, the problems of misjudgment and missed judgment easily occurring when the arc fault protector faces an unknown load combination can be solved.
Owner:FUZHOU UNIV
Method for simultaneously detecting residual quantity of 19 plant growth regulators in traditional Chinese medicinal materials
PendingCN119125358AGood linear relationshipimprove accuracyComponent separationBiotechnologyMedicinal herbs
The invention discloses a method for simultaneously detecting residual quantity of 19 plant growth regulators in traditional Chinese medicinal materials, and belongs to the technical field of traditional Chinese medicinal material detection. The 19 plant growth regulators in the detected traditional Chinese medicinal materials are gibberellins, 4-chlorophenoxyacetic acid, 6-benzyladenine, paclobutrazol, 3-indolebutyric acid, thidiazuron, 2, 4-dihydroxyacetophenone and 2, 4-dihydroxyacetophenone respectively. The pesticide is prepared from the following raw materials: sodium 4-dichlorphenoxyacetate, forchlorfenuron, 3-indoleacetic acid, 4-fluorophenoxyacetic acid, isopentenyl adenine, imazalil, N-N '-diphenylurea, mepiquat chloride, diethyl aminoethyl hexanoate, 1-naphthylacetic acid amine, brassin, abscisic acid and 3-indolepropionic acid. According to the method, the QUECHERS traditional Chinese medicinal material sample on-machine pretreatment technology is applied, the ultra-high performance liquid chromatography-tandem mass spectrometry is established to measure the residual quantity of the 19 plant growth regulators in the medicinal materials, and the detection method is simple, clear, few in operation steps, extremely easy to operate, good in linear relation, good in sensitivity and high in accuracy.
Owner:玉林市食品药品检验检测中心(玉林市药品不良反应监测中心)
Method for detecting porosity of battery pole piece
ActiveCN119290709AThe detection method is simplelow costPermeability/surface area analysisElectrical batteryPole piece
The invention discloses a method for detecting the porosity of a battery pole piece, and belongs to the technical field of batteries. The detection method comprises the following steps: S1, obtaining solid powder I of a coating material on a coated pole piece, obtaining a numerical value D50 in particle size distribution of the solid powder I, and recording the numerical value D50 as D1; s2, solid powder II of a coating material on the rolled pole piece is obtained, and the numerical value D50 in particle size distribution of the solid powder II is obtained and recorded as D2; s3, the porosity of the rolled pole piece is # imgabs 0 # # imgabs 1 #, wherein m + n = 1; m is the weight ratio of the active material in the solid powder I in the solid powder I; n is the weight ratio of the total weight of other components except the active material in the solid powder I in the solid powder I. The porosity detection method is simple and easy to implement, low in cost, short in time and capable of being popularized and used, and the accuracy rate can meet the use requirement.
Owner:JINGMEN YIWEI CHUANGNENG LITHIUM BATTERY CO LTD
CCDC171 gene molecular marker primer related to egg weight character and application of CCDC171 gene molecular marker primer
ActiveCN119287043AEfficient identificationImprove uniformityMicrobiological testing/measurementFood processingZ chromosomeGenotype
The invention relates to a CCDC171 gene molecular marker primer related to egg weight character and application of the CCDC171 gene molecular marker primer, and belongs to the technical field of biology. The CCDC171 gene molecular marker is located at the 32551904 base of a Z chromosome of a chicken reference genome GRCg7b version, the base is mutated into C or T, and the genotypes are C / C and T / T. And the egg weight of the C / C genotype chicken is higher than that of the T / T genotype individual. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for chicken breeding to breed chicken with proper egg weight; the egg weight character can be efficiently and accurately detected, the uniformity of the egg weight of chicken flocks is improved, and the method has important value for chicken breeding and production.
Owner:NANJING AGRICULTURAL UNIVERSITY +1
PPP3R1 gene molecular marker primer related to size character of cockscomb and application of PPP3R1 gene molecular marker primer
ActiveCN119307627AEfficient identificationImprove uniformityMicrobiological testing/measurementDNA/RNA fragmentationGenotypeSanger sequencing
The invention relates to a PPP3R1 gene molecular marker primer related to the size character of a cockscomb and application of the PPP3R1 gene molecular marker primer, and belongs to the technical field of biology. The molecular marker of the PPP3R1 gene is located at the 11347456 base of chromosome 3 of a chicken reference genome GRCg7b version, the base is mutated into T or G, and the genotypes are G / G, T / G and T / T. The cockscombs of the G / G genotype chickens are larger than those of the T / G and T / T genotype individuals, and the cockscombs of the T / G genotype chickens are larger than those of the T / T genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for chicken breeding to breed chicken with proper cockscomb size; the method can efficiently and accurately detect the size of the cockscomb of the chicken, improves the uniformity of the cockscomb of a group, and has important value for breeding and production of the chicken.
Owner:NANJING AGRICULTURAL UNIVERSITY
Clutch assembly detection device and detection method
ActiveCN119147251Aavoid measurement errorsSimplify the measurement stepsMachine part testingLaser rangingCoupling
The invention discloses a clutch assembly detection device and a detection method, and particularly relates to the technical field of detection equipment, the clutch assembly detection device comprises an equipment main body, the equipment main body comprises a fixed seat, a display screen, a torque measurement part, a distance measurement part and a pressure measurement part, the torque measurement part comprises a torque sensor, a coupler is fixedly connected to the torque sensor, and the distance measurement part is connected with the pressure measurement part. The distance measuring part comprises an annular plate, a plurality of connecting rods are arranged in the annular plate, the distance measuring part comprises a pushing frame, a baffle is arranged on the pushing frame, a supporting plate is arranged on the right side of the annular plate, a laser range finder is arranged at the left end of the supporting plate, the pressure measuring part comprises a release bearing, and a pressure sensor is arranged at the left end of the release bearing. According to the invention, the free stroke and separation stroke data of the clutch assembly can be visually measured, the measurement method is simplified, the detection precision and the detection efficiency are improved, and the output torque of the motor under different loads can be measured according to the displacement data variation.
Owner:平阳博翔汽车新能源科技有限公司
Kit for rapidly detecting respiratory pathogens by combining multiple PCR (Polymerase Chain Reaction) with CRISPR-Cas
PendingCN119082275AHigh detection throughputAchieve single-tube amplificationHydrolasesMicrobiological testing/measurementReverse transcriptaseDeoxyribose
The invention discloses a kit for rapidly detecting respiratory pathogens by combining multiple PCR (Polymerase Chain Reaction) with CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (Clustered Regularly Interspaced Short Palindromic Repeats). The kit comprises a multiple amplification primer combination, an sgRNA detection plate, DNA (Deoxyribose Nucleic Acid) polymerase, RNA reverse transcriptase, CRISPR / Cas The sequence of the multiple amplification primer combination is as shown in SEQ NO: 1-374; the sgRNA detection plate is an array type microporous plate, and each detection hole is filled with one sgRNA as shown in SEQ NO 383-569. The kit disclosed by the invention can be used for nucleic acid detection of respiratory pathogens and can be used for detecting viruses and bacteria at the same time, and the detection flux reaches up to 178 pathogens; the CRISPR-Cas is used for carrying out specific recognition on nucleic acid fragment targets in an amplification product, fluorescence detection is carried out, and the method has the advantages of being ultra-multiple, low in equipment requirement, low in cost, short in time, high in specificity, high in sensitivity and the like, and can provide molecular level judgment basis and help for clinical diagnosis of pathogen infection.
Owner:HANGZHOU MATRIDX BIOTECH CO LTD
Preparation method and application of ultra-sensitive electrochemical immunosensor for detecting MX1 protein based on nano composite material
InactiveCN119199113Aincrease the areaImprove conductivityMaterial electrochemical variablesEngineeringBovine serum albumin
The invention relates to the technical field of electrochemical immunosensors, in particular to a preparation method and application of an ultra-sensitive electrochemical immunosensor for detecting MX1 protein based on a nano composite material, and the preparation method comprises the following steps: S1, pretreating a glassy carbon electrode; s2, modifying with thiolated graphene oxide; s3, gold nanoparticle modification; s4, carrying out antibody immobilization; and S5, carrying out bovine serum albumin sealing treatment. The kit has ultrahigh sensitivity and high specificity, the detection method is simple, convenient and rapid, multiple signal amplification mechanisms and wide clinical application prospects are achieved, and early diagnosis and clinical monitoring of virus infection are facilitated.
Owner:NANJING TECH UNIV
Defect detection method and device for ceramic capacitor
ActiveCN119086596AThe detection method is simpleHigh precisionImage analysisCharacter and pattern recognitionCeramic capacitorComputer vision
The embodiment of the invention discloses a defect detection method and system for a ceramic capacitor. The method comprises the following steps: acquiring a target to-be-detected ceramic capacitor appearance image corresponding to a target to-be-detected ceramic capacitor; obtaining a target normal ceramic capacitor appearance image corresponding to the target normal ceramic capacitor with the normal appearance; performing same detection area division on the appearance image of the target normal ceramic capacitor and the appearance image of the target to-be-detected ceramic capacitor; judging whether a gray scale abnormal area of the target to-be-detected ceramic capacitor can be detected in the appearance image of the target to-be-detected ceramic capacitor or not; if yes, whether the similarity between the gray scale abnormal area of the target to-be-detected ceramic capacitor and the gray scale corresponding area of the target normal ceramic capacitor is smaller than or equal to the similarity threshold value of the appearance subareas of the target to-be-detected ceramic capacitor or not is judged; and if yes, determining that the appearance of the target to-be-detected ceramic capacitor has defects. The appearance defects of the ceramic capacitor can be detected, and the detection method is simple and high in precision.
Owner:SHENZHEN EYANG TECH DEV
Primer probe for rapid detection of hepatitis A virus RT-RPA and application and rapid detection method thereof
PendingCN119144769AWide coverageStrong specificityMicrobiological testing/measurementMicroorganism based processesHepatitis A virusesViral test
The invention belongs to the technical field of biology, and particularly relates to a primer probe for rapid detection of hepatitis A virus RT-RPA as well as application and a rapid detection method of the primer probe. The primer probe for the hepatitis A virus RT-RPA rapid detection is shown as SEQ ID NO: 1-3, RT-RPA-LFD rapid detection of the hepatitis A virus is established, the primer probe can be used for preparing a reagent for hepatitis A virus detection, rapid, high-sensitivity, high-specificity and macroscopic high-accuracy detection of the hepatitis A virus is achieved, and an efficient and convenient solution is provided for hepatitis A virus detection.
Owner:SHANGHAI OCEAN UNIV
Method, nucleic acid composition and kit for detecting toxoplasma gondii
PendingCN119530418ASpecific satisfactionmeet the accuracyMicrobiological testing/measurementMicroorganism based processesGondii toxoplasmaNucleotide
The invention discloses a method, a nucleic acid composition and a kit for detecting toxoplasma gondii, and relates to the technical field of toxoplasma gondii detection. The primer comprises an upstream primer and a downstream primer, the nucleotide sequence of the upstream primer and the sequence of SEQ ID NO: 1 have at least 90% identity, and the nucleotide sequence of the downstream primer and the sequence of SEQ ID NO: 2 have at least 90% identity. The nucleic acid composition for detecting the toxoplasma gondii provided by the invention aims at an APICO gene sequence of the toxoplasma gondii, and the sequence is a specific target region of the toxoplasma gondii. A fluorescent quantitative PCR or common PCR detection method established by using the target region can meet the requirements on specificity, accuracy and the like in a method verification process, and the method has important significance on in vitro diagnosis of toxoplasma gondii and in the veterinary field.
Owner:SANGON BIOTECH (SHANGHAI) CO LTD
A primer and probe set, kit, and application for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology.
ActiveCN121204310BOptimizing real-time fluorescence MIRA detection methodThe detection method is simpleMicrobiological testing/measurementMicroorganism based processesStaphylococcus aureusSingle strand
This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.
Owner:FRUIT TREE INST OF CHINESE ACAD OF AGRI SCI
Pseudo-ginseng component rapid detection method based on Proofman-LMTIA and application
PendingCN119287056AQuick checkAccurate detectionMicrobiological testing/measurementDNA/RNA fragmentationBiotechnologyPANAX NOTOGINSENG ROOT
The invention belongs to the technical field of molecular biology, and particularly relates to a pseudo-ginseng component rapid detection method based on a Proofman-LMTIA technology. According to the method, a pseudo-ginseng ITS2 sequence is taken as a specific target, and high-specificity and high-sensitivity detection of pseudo-ginseng components is realized through a designed LMTIA primer and a Proofman probe. Compared with the traditional detection technology, the invention has the advantages of simplified primer design, low-cost equipment requirement, rapid detection (only about 30 minutes) and wide application range. The method is not only suitable for detection of raw materials such as pseudo-ginseng seeds, seedlings, roots and rhizomes, decoction pieces and the like, but also can be used for authenticity identification of terminal products such as food, medicines, health care products and the like containing pseudo-ginseng components. Through innovative application of the Proofman-LMTIA technology, an effective solution is provided for rapid and accurate identification of pseudo-ginseng and derivative products thereof, and the method has important market application prospects and social benefits.
Owner:XUCHANG UNIV
Auxiliary device for bovine tuberculosis kit detection and use method thereof
PendingCN119552743AImprove timelinessimprove accuracyBioreactor/fermenter combinationsBiological substance pretreatmentsDiseaseCattle tuberculosis
The invention belongs to the technical field of biological detection, and particularly relates to an auxiliary device for bovine tuberculosis kit detection and a use method of the auxiliary device. The problems that existing bovine tuberculosis detection equipment is large in size, inconvenient to carry to the site, complex in operation, low in detection efficiency and high in detection cost are solved. According to the scheme, a plurality of vibration assemblies are arranged on a box body, the vibration assemblies are connected with a supporting assembly, the vibration assemblies are used for driving the supporting assembly to move up and down, a storage box is placed on the supporting assembly, a pore plate is arranged on the storage box, and a sample tube is placed on the pore plate; the auxiliary device for bovine tuberculosis kit detection has the advantages of being small in size, light in weight, easy and convenient to operate, convenient to carry, low in cost and the like so as to meet the requirement for on-site rapid detection, especially in areas with limited resources, the timeliness and accuracy of bovine tuberculosis diagnosis are improved, prevention and control measures can be taken in time, and the detection efficiency is improved. Disease transmission risks are reduced, and healthy development of animal husbandry is guaranteed.
Owner:大理市畜牧兽医中心
SH3GL3 gene molecular marker primer related to egg weight character and application of SH3GL3 gene molecular marker primer
ActiveCN119265323AEfficient identificationImprove uniformityMicrobiological testing/measurementFood processingAnimal scienceMedicine
The invention relates to an SH3GL3 gene molecular marker primer related to egg weight character and application of the SH3GL3 gene molecular marker primer, and belongs to the technical field of biology. According to the present invention, the genome DNA of the chicken to be detected is adopted as the template, the specific primer is adopted to perform PCR amplification on the template, the PCR amplification product is subjected to Sanger sequencing and SNP molecular marker gene typing, and the 52-week-old egg weight character selection of the chicken can be achieved based on the genotype of the SNP molecular marker; by eliminating A / G and A / A genotype individuals and retaining G / G genotype individuals, the molecular marker has the beneficial effects that the molecular marker can be used as a genetic marker for chicken breeding to breed 52-week-old chickens which are relatively small and uniform in egg weight; the method can be used for efficiently and quickly identifying the egg weight character of the chickens at the age of 52 weeks, improves the uniformity of the egg weight of chicken flocks, provides a scientific basis for early breeding of the chickens, and has an important value for chicken breeding. In addition, the detection method disclosed by the invention is simple and easy to operate, and can be carried out in a laboratory.
Owner:NANJING AGRICULTURAL UNIVERSITY
Clutch assembly detection device and detection method
ActiveCN119147251BSimplify the measurement stepsavoid measurement errorsMachine part testingLaser rangingCoupling
The invention discloses a clutch assembly detection device and a detection method, which specifically relate to the technical field of detection equipment, including an equipment main body, the equipment main body including a fixed seat and a display screen, a torque measuring part, a distance measuring part and a pressure measuring part, the torque measuring part including a torque sensor, a coupling is fixedly connected to the torque sensor, a rotating shaft is fixedly connected to the fixed seat, the distance measuring part includes an annular plate, a plurality of connecting rods are arranged in the annular plate, the distance measuring part includes a pushing frame, a baffle is arranged on the pushing frame, a support plate is arranged on the right side of the annular plate, a laser rangefinder is arranged at the left end of the support plate, the pressure measuring part includes a release bearing, and a pressure sensor is arranged at the left end of the release bearing. The invention can directly measure the free stroke and release stroke data of the clutch assembly, simplify the measurement method, improve the detection accuracy and detection efficiency, and can measure the output torque of the motor under different loads according to the displacement data change.
Owner:平阳博翔汽车新能源科技有限公司
Vehicle-mounted display screen lens falling detection device
ActiveCN222528912UAchieve all-round detection of anti-fallno risk of sheddingInternal combustion piston enginesApparatus for force/torque/work measurementIn vehicleMechanical engineering
Owner:WEIHAI JITONG OPTICAL TECHNOLOGY CO LTD
Linear guide rail pitch detection device
ActiveCN222481354UGuaranteed accuracyImprove detection accuracyMechanical clearance measurementsControl engineeringControl theory
The utility model relates to the technical field of pitch detection, and particularly discloses a linear guide rail pitch detection device which comprises a base and a positioning mechanism, a first sliding rail is arranged at the upper end of the base, stand columns are arranged at the two ends of the first sliding rail, and a graduated scale is arranged between the two stand columns. The positioning mechanism comprises a positioning rod and a first sliding block matched with the first sliding rail, the upper end of the first sliding block is connected with a supporting block, the positioning rod is in vertical sliding fit with the supporting block, the lower end of the positioning rod is provided with a positioning cone matched with a linear guide rail installation hole, and the upper end of the first sliding block is connected with an indicating rod. The indicating rod is tightly attached to the graduated scale, the side wall of the indicating rod is provided with indicating lines used for indicating scales, and the indicating lines correspond to the front and back positions of the center line of the positioning cone. According to the utility model, the automatic centering of the mounting hole during detection is realized, the accuracy of the detection position is ensured, the detection precision is improved, the detection mode is simple, the operation is convenient, and the detection efficiency is improved.
Owner:山东台稳精密机械有限公司
Vehicle-mounted camera attachment detection method, system and terminal
PendingCN119251164Aimprove accuracyStrong real-timeImage analysisCharacter and pattern recognitionComputer graphics (images)In vehicle
The invention provides a vehicle-mounted camera attachment detection method, system and terminal, and the method comprises the steps: obtaining continuous video frames collected by a vehicle-mounted camera, and storing the continuous video frames in a frame list; preprocessing each video frame in the frame list; performing difference detection on all the preprocessed adjacent video frames, and identifying a pixel invariant region between the adjacent video frames based on a difference detection result; and carrying out multi-frame similarity comparison on the pixel invariant region to detect the existence of the attachment. The vehicle-mounted camera attachment detection speed and accuracy can be effectively improved, meanwhile, the requirement for computing resources is reduced, and the vehicle-mounted camera attachment detection method has the advantages of being high in detection precision, high in real-time performance, high in robustness, low in implementation cost, high in reliability and the like.
Owner:SHANGHAI BAOLONG AUTOMOTIVE CORP (WUHAN) CO LTD
Porphyromonas gingivalis detection method based on portable glucometer
PendingCN119290985AGuaranteed recognition effectGuaranteed detection effectMaterial electrochemical variablesBiotechnologyBlood glucose meters
The invention relates to the field of pathogen detection, in particular to a porphyromonas gingivalis detection method based on a portable glucometer. The method provided by the invention comprises the following steps: S101, adding a sample into a first reaction system and incubating to obtain a first mixture; s102, a modified matrix electrode is in contact with the first mixture and then washed; s103, inserting the washed modified substrate electrode into a blood glucose meter; s104, obtaining a glucometer reading, and further obtaining the concentration of the porphyromonas gingivalis in the sample; wherein the modified matrix electrode is modified by an Au (at) V4C3MXene material and is combined with an antibody for specifically recognizing porphyromonas gingivalis and a sealing agent. The detection method provided by the invention is sensitive, rapid, stable, accurate and simple, and accurate detection of the single-digit porphyromonas gingivalis in an actual sample can be realized in a relatively short time (such as 20 minutes).
Owner:STOMATOLOGICAL HOSPITAL OF CHONGQING MEDICAL UNIV
Microelement water-soluble fertilizer measuring instrument
ActiveCN119290785AThe detection method is simpleeasy to useColor/spectral properties measurementsAgricultural scienceTrace element
The invention discloses a trace element water-soluble fertilizer measuring instrument, and relates to the technical field of fertilizer detection. According to the trace element water-soluble fertilizer measuring instrument, different raw materials can be put into corresponding conical discharging grooves through the cooperation of a discharging hopper and a bearing disc, then the different raw materials can be discharged through discharging pipes in sequence through the cooperation of a communicating air pipe and a telescopic corrugated pipe, and through the cooperation of the bearing disc, the trace element water-soluble fertilizer can be measured. According to the detection device, the multiple limiting frames and the detection test tubes can be driven to operate when raw materials sequentially fall until the raw materials enter the corresponding detection test tubes, compared with a traditional detection mode, the detection device is more convenient and faster to use, detection operation of the multiple raw materials can be completed, and the use effect and the operation efficiency of equipment are effectively improved.
Owner:SHANDONG HUANGJIA DIGUO BIOTECHNOLOGY CO LTD
LMX1B gene molecular marker primer related to egg weight character and application of LMX1B gene molecular marker primer
ActiveCN119287042AImprove uniformityEfficient identificationMicrobiological testing/measurementFood processingLMX1B geneEggs per gram
The invention relates to an LMX1B gene molecular marker primer related to egg weight character and application of the LMX1B gene molecular marker primer, and belongs to the technical field of biology. The molecular marker of the LMX1B gene is located at the 10705046th base of chromosome 17 of a chicken reference genome GRCg7b version, the base is mutated into T or G, and the genotypes are G / G, G / T and T / T. The egg weight of the G / G genotype chicken is higher than that of the G / T and T / T genotype individuals, and the egg weight of the G / T genotype chicken is higher than that of the T / T genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing so as to rapidly and accurately identify the genotype of the individual; the molecular marker can be used as a genetic marker for chicken breeding to breed chicken with proper egg weight; the egg weight character can be efficiently and accurately detected, the uniformity of the egg weight of chicken flocks is improved, and the method has important value for chicken breeding and production.
Owner:NANJING AGRICULTURAL UNIVERSITY
Application of neutrophil number level in prediction of tumor treatment effect
InactiveCN119144688Agood treatment effectThe detection method is simpleCompound screeningApoptosis detectionDiseaseHead and neck tumors
The invention discloses application of neutrophile granulocyte number level in prediction of tumor treatment effect. Through verification of the embodiment of the invention, the number level of neutrophils in blood of a tumor patient before treatment is closely associated with the curative effect of tumor treatment, especially OH2 treatment, and the applicable tumor adaptation diseases are common; comprising at least one of melanoma, esophageal cancer, right breast invasive ductal cancer, intestinal cancer, head and neck tumor, gastric cancer, ovarian cancer, biliary system tumor, left foot back squamous cell carcinoma, sarcoma and osteosarcoma, lung cancer, thymosquamous cell carcinoma and brain glioma. In the application disclosed by the invention, the threshold value of the number of neutrophils for predicting the tumor treatment effect is 3.5 * 10 < 9 > / L; the treatment is predicted to be effective when the number of sexual granulocytes in a tumor patient is below 3.5 * 109 / L before the tumor patient is treated, and the treatment is predicted to be ineffective or invalid when the number of sexual granulocytes in the tumor patient is higher than 3.5 * 109 / L. The application method is simple, low in cost and good in stability, and can become a potential prediction means for clinicians.
Owner:WUHAN BINHUI BIOTECH CO LTD
CDC34 gene molecular marker primer related to egg weight character and application of CDC34 gene molecular marker primer
ActiveCN119265325AImprove uniformityrapid identificationMicrobiological testing/measurementFood processingPhysiologyGenotype
The invention relates to CDC34 gene molecular marker primers related to egg weight characters and application of the CDC34 gene molecular marker primers, and belongs to the technical field of biology. The molecular marker is located at the 3100727th basic group of a chicken reference genome GRCg7b version 28 # chromosome, the basic group is mutated into A and G, and the genotypes are A / A, A / G and G / G. The 31-week-old egg weight of the A / A genotype chicken is higher than that of A / G and G / G genotype individuals, and the 31-week-old egg weight of the A / G genotype chicken is remarkably higher than that of the G / G genotype individuals. According to the present invention, the forward and reverse primer pair is used to amplify the fragment containing the molecular marker site, and the amplification product is subjected to Sanger sequencing, such that the individual genotype can be rapidly and accurately identified. The molecular marker can be used as a genetic marker for chicken breeding to breed 31-week-old chicken with proper egg weight; the 31-week-old egg weight character of the chicken can be efficiently and accurately detected, the uniformity of the egg weight of chicken flocks is improved, and the method has important value for breeding and production of the chicken.
Owner:NANJING AGRICULTURAL UNIVERSITY
Method for rapidly detecting odor active substances in fish meat
PendingCN119086781ATo achieve the purpose of quantifying volatile componentsEasy to detectComponent separationBiotechnologyAnimal science
The invention provides a method for rapidly detecting odor active substances in fish meat, and belongs to the technical field of detection of the odor active substances in the fish meat. The method for rapidly detecting the odor active substances in the fish meat comprises the following steps: mixing the fish meat, a saturated sodium chloride solution and 2, 4, 6-trimethylpyridine, heating at 85 DEG C for 40 minutes, and extracting by a headspace solid-phase microextraction method; and performing gas chromatography-mass spectrometry, identifying the compounds, calculating to obtain the content of each volatile compound component relative to the internal standard, calculating to obtain OAV, and screening to obtain the odor active substances in the fish meat according to the condition that OAV is greater than or equal to 1. According to the method, the odor active substances in the sample are screened by combining the quantitative value of each volatile component by the internal standard with the odor threshold value of each substance, and the compound which really contributes to the overall odor of the fish meat can be determined. In addition, by designing different extraction temperatures and extraction time, the odor active substances in the fish meat can be simply, conveniently and quickly detected and obtained.
Owner:TONGWEI AGRI DEV CO LTD
Ratio fluorescence sensor based on fluorescein isothiocyanate labeled metal organic framework and application of ratio fluorescence sensor in escherichia coli detection
InactiveCN119464441ADecreased fluorescence intensityRealize quantitative detectionMicrobiological testing/measurementMicroorganism based processesMetal-organic frameworkUltraviolet lights
The invention discloses application of a ratiometric fluorescence sensor based on a fluorescein isothiocyanate labeled metal organic framework in escherichia coli detection. The method comprises the following steps: synthesizing ZTMs by taking TPPS as a ligand, and then fully incubating with FITC to prepare ZTMs (at) FITC; according to the present invention, Escherichia coli is used to reduce Cu < 2 + > to Cu < + >, such that the fluorescence intensity of ZTMs (at) FITC is changed, the fluorescence intensity of the emission peak at the 515 nm position is reduced, the fluorescence intensity of the emission peak at the 683 nm position is increased, and the color of the solution is observed to gradually change from yellow to orange and finally to red under the 365 nm ultraviolet light; and rapid and sensitive detection of the escherichia coli is realized through the ratio of the fluorescence intensities of the two emission peaks. According to the detection method, the accuracy of a detection result is improved, errors caused by environment, instruments and manual operation can be avoided, professional operators are not needed, the method has the advantages of being rapid, simple and independent of expensive instruments and equipment, and the detection cost is reduced.
Owner:CHANGZHOU UNIV
Steel pipeline defect detection method and device
ActiveCN119510553AThe detection method is simpleHigh precisionMaterial magnetic variablesSteel pipelineMechanical engineering
The invention provides a steel pipeline defect detection method and device. The steel pipeline defect detection method comprises the following steps: S1, detecting by using a transient electromagnetic method: transmitting a transient electromagnetic excitation signal to a steel pipeline, generating a transient electromagnetic attenuation signal after the surface of the steel pipeline is excited by the transient electromagnetic excitation signal, and receiving the transient electromagnetic attenuation signal generated on the surface of the steel pipeline; s2, weak magnetic detection signals generated on the surface of the steel pipeline are detected through a weak magnetic method; and S3, performing fusion enhancement processing on the transient electromagnetic attenuation signal and the weak magnetic detection signal. The steel pipeline defect detection device comprises a weak magnetic detection device, a transient electromagnetic detection device and a data fusion upper computer for fusing weak magnetic detection signals and transient electromagnetic signals. According to the invention, nondestructive testing is realized by measuring electrical variables or magnetic variables.
Owner:INNER MONGOLIA RESEARCH INSTITUTE CHINA UNIVERSITY OF MINING AND TECHNOLOGY (BEIJING) +2