36results about How to "The detection method is simple" patented technology

This invention discloses a primer and probe set, a kit, and their applications for detecting Staphylococcus aureus L based on real-time fluorescence MIRA technology. The primer and probe set consists of single-stranded DNA sequences 1-3. Based on the aforementioned primer and probe set, this invention establishes and optimizes a real-time fluorescence MIRA detection method for Staphylococcus aureus L. Furthermore, the optimized real-time fluorescence MIRA method is innovatively combined with a simplified nucleic acid extraction method and a portable fluorescence detection device, significantly reducing extraction and time costs while enabling rapid on-site detection of the virus, making it particularly suitable for grassroots units and remote areas. In addition, the real-time fluorescence MIRA detection method for Staphylococcus aureus L established in this invention has advantages such as high specificity, high sensitivity, and simple operation. Moreover, the detection process does not require opening the lid, effectively avoiding aerosol contamination, providing an effective technical means for the differential diagnosis and rapid detection of Staphylococcus aureus L.

The utility model discloses a kind of sheet metal cabinet case size detection devices of laser ranging, it is related to cabinet case measurement technical field, including base, base is provided with arc hole;Support frame sliding limit is in arc hole, support frame bottom is connected with rotating member, detection component includes sliding part, overturning part and detection part;L-shaped positioning block is positioned with the corner of the cabinet case to cooperate sliding part, overturning part, detection part and rotating member to detect the size of cabinet case.The utility model solves the higher cost, the difficulty of debugging and data measurement inaccuracy caused by the need of multiple laser range finders to complete the detection device device of existing, by rotating member, the cooperation of sliding part, overturning part and L-shaped positioning plate position cabinet case, can realize only by one detection part completes the measurement of cabinet case size, and detection mode is simple and efficient.

The invention relates to the technical field of biology, in particular to an SNP (Single Nucleotide Polymorphism) molecular marker related to soybean mosaic virus resistance, an amplification primer and application of the SNP molecular marker in soybean breeding. The SNP molecular marker is located at the 910489bp site of a No.7 chromosome of a soybean genome, and the polymorphism of the SNP molecular marker is C / A. The invention provides application of the SNP molecular marker related to the soybean mosaic virus resistance in identification or auxiliary identification of the soybean mosaic virus resistance. By providing the accurate, simple, convenient and low-cost SNP molecular marker and the detection method thereof, early-stage, rapid and efficient molecular-assisted selection of soybean mosaic virus resistance characters is realized, so that the breeding process of a new variety of disease-resistant soybeans can be remarkably accelerated, and the SNP molecular marker has important breeding application value.

This invention relates to a digital radiographic inspection method and apparatus for wind turbine blades, belonging to the field of wind turbine blade inspection technology. The method includes: placing and fixing the turbine blade on a blade fixture; mounting the X-ray machine on the X-ray machine fixture; defining a detection grid in the inspection area of ​​the turbine blade; tightly attaching and fixing the X-ray flat panel detector to the turbine blade; and sequentially inspecting each grid of the turbine blade according to the detection grid. This invention, through the combination of the blade fixture, the X-ray machine fixture, the X-ray machine, and the X-ray flat panel detector, constitutes a digital radiographic inspection apparatus for turbine blades. This apparatus enables the detection of defects in wind turbine blades. The inspection method of this invention is simple and rapid; the inspection images obtained by this invention can clearly and accurately identify defects in wind turbine blades, with a defect detection rate of 100%.

The application aims to provide a dielectric property testing system and method of a wave-transparent material under high temperature and high pressure, and belongs to the technical field of microwave and millimeter wave dielectric material testing. The system innovatively adds a temperature device for heating and a pressure device for pressurizing in a conventional quasi-optical cavity method testing device, and sets a fused quartz considering the influence of temperature and pressure on the optical cavity, so that the system can test the dielectric property of the wave-transparent material under high temperature and high pressure by using the quasi-optical cavity method. Meanwhile, the system is simple to operate and has high testing precision.

The application discloses a SNP molecular marker related to the content of oleic acid in sesame seeds and application, and belongs to the technical field of molecular marker breeding. The SNP molecular marker related to the content of oleic acid in sesame seeds is located at 2754308 bp of the No. 3 chromosome, the genotype GG corresponds to the content of oleic acid which is significantly higher than that of AA (the difference reaches the extremely significant level of 0.01), and the accurate differentiation of high / low oleic acid germplasm can be realized. Early genotype identification can be carried out at the seedling stage of sesame by using the marker, and the breeding cycle is greatly shortened and the selection efficiency is improved without waiting for seed maturation. The marker is stable and reliable under different genetic backgrounds, is less affected by the environment, and is simple, high-flux and low-cost in detection, is suitable for large-scale automatic screening, can be directly used for molecular marker assisted selection of high-oleic-acid sesame varieties, accelerates the cultivation of high-quality new varieties, and has important application value for improving the sesame oil quality and market competitiveness.

This utility model relates to a novel high-voltage cable coupler, comprising a coupler plug, a coupler socket, a measurement module, and an energy harvesting module. Windows and / or aviation sockets are provided on both the coupler plug and the coupler socket. The measurement module and the energy harvesting module are encapsulated within the entire cable coupler. The high-voltage cable coupler in this design has windows for wireless communication, optical detection, and image recognition functions. This design detects the ambient temperature and humidity, arc light, contact temperature, electrical quantities, and internal temperature of the high-voltage cable coupler, providing support for intelligent monitoring and status judgment, fault detection, and location of the high-voltage cable coupler. The energy harvesting module in this design features adaptive energy management, adapting to various complex environments, eliminating the need for an external power source, freeing it from energy constraints, improving equipment autonomy, reliability, and sustainability, and reducing maintenance costs.

The present application provides a SNP molecular marker related to wet damage tolerance of Brassica napus and application thereof, wherein the sequence of the SNP molecular marker is shown as SEQ ID No. 1, and an A / G base mutation exists at 11872958 of the sequence shown as SEQ ID No. 1. The SNP molecular marker provided by the present application is based on a competitive allele-specific PCR marker detection method, and is used for detection and identification of the wet damage tolerance trait of Brassica napus. The detection method is simple in operation, low in cost, accurate in detection result, good in repeatability and stability, and different detection laboratories and different data results can be compared and verified with each other, and the data has universal comparability.

The application of the biomarker combination in the preparation of a drug for fulminant myocarditis belongs to the field of drugs. The application is characterized in that the biomarker combination comprises Siglec-5; the biomarker combination is further selected from the group consisting of sST2, PAI-1, Siglec-5, CD163, CD40, P-Cadherin, CD14, CTLA4. The application finds that the expression of the biomarker in the plasma of a patient with fulminant myocarditis is increased, and confirms the role of the biomarker as a drug target in relieving and / or improving fulminant myocarditis. The level of the biomarker can be controlled by a drug, the diagnosis and subsequent treatment of the disease can be more intensified, and the biomarker has great application value in clinical practice.

This invention discloses the application and method of metabolic biomarkers. It includes the application of metabolic biomarkers in the preparation of drugs for detecting the efficacy of immunotherapy for liver cancer, and a method for using metabolic biomarkers to detect the efficacy of immunotherapy for liver cancer. This invention uses metabolic biomarkers with high specificity, universality, and reliability as markers, and animal experiments have demonstrated that specific inhibition of the metabolic biomarkers of this invention can enhance the efficacy of immunotherapy for liver cancer, showing clinical translational potential.

The application discloses a fault detection method, device and equipment of a variable pitch system, and a readable storage medium. The variable pitch system can obtain a blade angle and a reference angle when a proximity switch is triggered, compare the blade angle with the reference angle, update forward counting data and / or negative counting data corresponding to the proximity switch according to a comparison result, and determine a fault type of the variable pitch system according to the updated forward counting data and negative counting data. According to the application, the blade angle and the reference angle when the proximity switch is triggered are used for fault detection, compared with fault detection according to a switch signal of the proximity switch, the problem that fault detection is not timely due to signal delay is avoided, and the application only needs to use two counting data to complete fault detection of the variable pitch system, so that the detection method is simple and reliable, and complex model operation is not needed.

The application provides a kit for detecting penicillin and amoxicillin resistance of streptococcus suis and application thereof, and belongs to the technical field of molecular biology detection. The kit comprises a primer pair and AlwNI restriction endonuclease; the primer pair comprises primers PBP1a-2083F and PBP1a-2083R, and the sequences are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The kit is used for detecting penicillin and amoxicillin resistance of streptococcus suis, has the advantages of high specificity, high sensitivity, low detection cost and low technical requirement, and provides reliable technology for monitoring penicillin and amoxicillin resistance of streptococcus suis in clinic.

The present application relates to attosecond strong field physics technical field, especially point to a kind of method based on photoelectron holographic interferometry detection intramolecular electron migration movement, including using mid-infrared or near-infrared femtosecond laser ionizes ground state electron wave packet in molecule, obtains first electron momentum distribution;Using far ultraviolet femtosecond laser acts on molecule ground state, obtains the superposition of ground state and excited state electron wave packet, using mid-infrared or near-infrared femtosecond laser ionizes intramolecular superposition state electron wave packet, obtains second electron momentum distribution;Obtain the momentum deviation of holographic interference in the first and second electron momentum distribution of both;Obtain the change of electron wave packet most probable density distribution with time at the exit of molecular tunneling ionization, and the proportion of excited state in superposition state electron wave packet.The present application realizes the detection of intramolecular electron migration movement, and the detection method is more efficient and direct, the result obtained is more accurate, the feasibility and applicability are stronger, and has practical value in the detection of ultrafast electron dynamics process.

The utility model discloses a reminding system of a vehicle-mounted charger, which comprises a detection module, a DC / DC conversion module, an energy storage module and an alarm module, a first input end of the reminding system is respectively connected with the detection module and the DC / DC conversion module, the DC / DC conversion module is connected with the energy storage module, and the energy storage module is connected with the alarm module. The detection module is used for detecting the level change of an input signal and an output signal and detecting the condition that the vehicle-mounted charger is connected with a load according to the level change, the detection module is connected with the alarm module, and the alarm module is used for giving an alarm when the vehicle-mounted charger is connected with the load. By adopting the detection module, the DC / DC conversion module, the energy storage module and the alarm module, when the mobile phone is in vehicle-mounted charging and an automobile flames out, a prompt alarm is given out to prevent the mobile phone from falling into the automobile, the detection mode is simple, and the cost is saved by replacing an NFC mode with the detection module and the alarm module.

The application discloses a kit for detecting cat feces related circular virus and application thereof, and belongs to the technical field of DNA virus detection. The detection kit can significantly improve the detection flux and timeliness of the cat feces related circular virus, has high detection sensitivity and strong specificity, can effectively solve the problem of low FeSCV virus load in healthy cats and easy missed detection, has clear components, low device dependency, and the color change can be directly observed by naked eyes under natural light to read the result, greatly simplifies the operation process, reduces the personnel operation error, and improves the reliability of the detection result, has low requirement on professional knowledge, is particularly suitable for use in primary pet clinics, cat houses / farms, isolation quarantine areas and field epidemiological investigation, can simultaneously detect multiple geographical strains of FeSCV such as Chinese epidemic strains and Japanese reference strains, can realize consistent detection of different geographical strains, and provides a reliable tool for the epidemiological monitoring of FeSCV.

The invention discloses a lead-acid battery electrolyte detection method based on sensor layout and light and shade signals, which comprises the following steps: acquiring the voltage of a lead-acid battery by using a voltage sensor, outputting a corresponding signal to a first high-low level conversion module, comparing the corresponding signal with a lower limit value of the voltage of the lead-acid battery, and outputting a first level signal; a liquid level height-density sensor is used for collecting height information of the liquid level of the lead-acid battery electrolyte, outputting a corresponding signal to a second high-low level conversion module to judge whether the liquid level height of the electrolyte is lower than the standard liquid level height of the electrolyte or not, and outputting a second level signal; collecting the density information of the electrolyte by using a liquid level height-density sensor, outputting a corresponding signal to a third high-low level conversion module to judge whether the density of the electrolyte is lower than the standard density of the electrolyte, and outputting a third level signal; and the light and shade signal logic judgment module is used for receiving the three level signals and outputting light and shade signals to determine the detection result of the lead-acid battery electrolyte. The sensor layout is simplified, and light and dark signal detection is simple and effective.

The invention discloses an on-line haze feedback production method for a nuclear track membrane. The method comprises the following steps: controlling the advancing speed of a membrane material through a winding and unwinding device; etching the membrane material through an etching device to obtain a nuclear track membrane; cleaning the etching liquid on the nuclear track membrane through a cleaning device; drying the cleaned nuclear track membrane through a drying device; detecting the nuclear track membrane through haze detection equipment to obtain a real-time haze detection result and a real-time light transmittance detection result; and according to the real-time haze detection result and the real-time light transmittance detection result, operating parameters of the winding and unwinding device and the etching device are adjusted in real time. The method can achieve the real-time detection of the thickness and pores of the nuclear track membrane on a production line through haze and light transmittance detection, facilitates the automatic feedback production, is simple in detection method, and is high in detection speed. Equipment cost can be greatly saved, production efficiency is improved, and production waste is reduced.

The utility model discloses a bearing axial clearance detection mechanism, which comprises a detection base, a lifting assembly, a pressure applying assembly and a detection part, a bearing table is arranged on the detection base, the bearing table is used for bearing an outer ring of a bearing to be detected, a notch is formed in the bearing table, and the lifting assembly extends into the notch and abuts against the bottom of an inner ring. The pressing assembly comprises a first driving piece and a pressing piece, a pressing protrusion is arranged at the bottom end of the pressing piece, the first driving piece is used for driving the pressing piece to rise to a high position or a low position, and the first driving piece drives the pressing piece to the low position, so that the pressing piece abuts against the upper end face of the outer ring and pushes the inner ring on the upper end face of the inner ring in cooperation with the pressing protrusion; and the detection part is used for performing axial clearance detection on the to-be-detected bearing when the pressing bulge pushes the inner ring. According to the bearing axial clearance detection mechanism, through cooperation of the lifting assembly, the pressure applying assembly and the detection part, bearing axial clearance detection is achieved, the detection mode is convenient and fast, the structure is simple, the detection efficiency is high, and the detection precision is high.

The invention discloses a liquid level alarm device for a fire-fighting tank, and relates to the technical field of liquid level alarm equipment, the liquid level alarm device comprises a power supply, a hollow shaft and an annular electrifying plate, the power supply is fixedly mounted on one side of the top of the tank, the top of the power supply is electrically connected with a display control panel, and a controller and an alarm are arranged in the display control panel; the three hollow shafts are rotationally installed in the tank body in an annular array mode, the outer sides of the hollow shafts are fixedly connected with oval electrifying feeler levers in a sleeving mode, and meshing transmission parts are connected between the top ends of the multiple hollow shafts and a control handle of the tank body; the annular electrifying plate is fixedly installed in the middle of the top of the tank body, one end of the annular electrifying plate is electrically connected with a power source, and the other end of the annular electrifying plate penetrates through the tank body and is electrically connected with the electrifying feeler levers. According to the invention, whether gas leakage or deflation exists in the tank body is alarmed and prompted, the detection mode is simple and timely, and the precision is high.

The present disclosure relates to a welding deviation defect detection method, device, system and program product, and relates to the technical field of welding defect detection. The welding deviation defect detection method comprises: determining a frequency domain feature of real-time welding parameters of a product according to the real-time welding parameters; determining a first target frequency and a second target frequency according to target welding parameters of the product, characteristic parameters of the product and a welding gun swing frequency; determining a first frequency domain parameter corresponding to the first target frequency and a second frequency domain parameter corresponding to the second target frequency according to the first target frequency, the second target frequency and the frequency domain feature; and detecting whether there is a welding deviation defect according to the first frequency domain parameter, the second frequency domain parameter and the frequency domain feature.

This application discloses an intelligent detection and analysis system for gear surface morphology, which includes an image acquisition unit for acquiring images of the gear surface inside a gearbox. The system acquires these images and extracts surface morphology feature values ​​to generate gear surface morphology feature recording data. It also acquires cumulative unit operating time, cumulative unit power generation, gearbox usage time, and gearbox failure operating time to generate gear operating history data. Combining the gear surface morphology feature recording data and the gear operating history data, a gearbox life analysis model is established using a deep learning algorithm to analyze and calculate the gearbox life. This intelligent detection and analysis system for gear surface morphology has the advantages of convenient and simple detection methods and higher accuracy compared to purely manual inspection.

A norfloxacin sensor and its preparation and detection methods are disclosed, relating to the field of electrochemical sensor technology. The preparation method includes: S1 preparing a CTS / Ag-NiWO4 composite material; S2 preparing a multi-walled carbon nanotube solution; S3 polishing and activating the surface of a base electrode; S4 preparing a first solid film on the surface of the base electrode using the multi-walled carbon nanotube solution; and S5 preparing a second solid film on the surface of the first solid film using the CTS / Ag-NiWO4 composite material. This invention utilizes multi-walled carbon nanotubes, Ag-NiWO4, and chitosan to construct the sensor interface, enabling quantitative detection of norfloxacin concentration. It not only exhibits strong adsorption, catalytic, and conductivity capabilities, but also boasts a simple and easy-to-implement preparation and detection method, high sensitivity, and a low detection limit.

The invention discloses a method for detecting food-borne pathogenic bacteria based on Argonaute protein in combination with loop-mediated isothermal amplification and electrochemical biosensing, and belongs to the technical field of microbiological detection and biosensing. The integrated detection method based on Argonaute protein, loop-mediated isothermal amplification and electrochemical biosensing, which is high in sensitivity, strong in specificity, simple and convenient to operate and suitable for on-site rapid detection, is provided aiming at the problems that an existing food-borne pathogenic bacterium detection method is insufficient in sensitivity, depends on complex instruments, is difficult to apply on site and the like. Target nucleic acid is subjected to exponential amplification through LAMP (loop-mediated isothermal amplification), then Argonaute-mediated two-stage specific recognition and signal transduction are performed, and finally reading is performed through a high-sensitivity electrochemical platform, so that the detection limit of salmonella typhimurium as high as 1 CFU / mL is realized, and the detection range can cover 100-108 CFU / mL.

The invention relates to rare earth doped down-conversion nanoparticles, a preparation method thereof and application of the rare earth doped down-conversion nanoparticles in detection of quinolone antibiotics, and belongs to the technical field of chemical materials and food detection. The rare earth doped down-conversion nano-particle comprises an inner core, a shell and a coating layer, wherein the shell is positioned between the inner core and the coating layer; the inner core contains NaGdF4: Ce / Eu / Ca, the shell contains NaTbF4, and the coating layer contains SiO2; the NaGdF4: Ce / Eu / Ca is a material which takes NaGdF4 as a matrix and is doped with Ce, Eu and Ca. According to the invention, the hydrophilicity and dispersity of the nanoparticles are improved, and the enrichment and detection limits of norfloxacin are improved. The fluorescent probe has the characteristics of narrow-band emission, long fluorescence lifetime, excellent light and chemical stability, low biotoxicity and the like, and the detection method is simple and convenient.

The invention discloses a method for specifically detecting poecilobdella glauca and application of the poecilobdella glauca. The primer probe group capable of specifically detecting the poecilobdella glauca is designed on the basis that the ND1 gene of the poecilobdella glauca serves as a target, the detection sensitivity reaches 2.88 * 100 copies / mu L, and meanwhile high repeatability and negative stability are achieved. The invention provides a new molecular target for detecting the poecilobdella glauca, and provides a simpler and more convenient detection means for disease detection and monitoring of the poecilobdella glauca in the aquaculture process.

The present application relates to the technical field of cable protection pipe on-site nondestructive testing, and discloses a kind of cable protection pipe air hole and crack fast nondestructive testing method and system.It includes the comprehensive detection method of air tightness detection and crack detection, and the air tightness detection device includes upper cover plate, lower cover plate, sealing strip, first sealing ring, second sealing ring, cable protection pipe, screw, nut, air pipe, rubber hose, U-shaped communicating vessel, hand-operated screw propeller, and the sealing property of cable protection pipe is determined by observing the scale rise and fall of liquid in U-shaped communicating vessel.Under the condition of detecting no air hole, the crack detection adopts coloring penetration flaw detection method to detect the crack of cable protection pipe, and the crack in cable protection pipe is developed by using penetrant, developer and cleaning agent, and the quality is determined whether it is qualified or not.The device structure is simple, convenient to operate, does not need power system, can save time, energy and cost.

This utility model relates to the field of testing platform technology, specifically to a testing platform for the perpendicularity of a cylinder. It includes a fixed base with two first rotating slots at its top. A first rotating shaft and a second rotating shaft are rotatably connected to the two first rotating slots, respectively. A storage tray is fixedly connected to one end of each first rotating shaft extending through the first rotating slots to the outside of the fixed base. A magnet is fixedly connected to the inner cavity of the upper end of the storage tray. A metal cylinder is placed in the middle of the storage tray. A threaded sleeve is fixedly connected to one end of the second rotating shaft extending through the first rotating slots to the outside of the fixed base. A threaded rod is threadedly connected to the inside of the threaded sleeve. Compared to existing technologies, this application offers a simpler testing method, is less affected by the end face area of ​​the metal cylinder, improves the accuracy of detecting the perpendicularity of the metal cylinder's end face, and allows the dial indicator to measure metal cylinders with various height and diameter values, thus enhancing the practicality of the testing platform.

The application discloses a nucleic acid aptamer specifically recognizing alpha-amatoxin and screening and application thereof, and belongs to the technical field of biology. The application discloses nucleic acid sequences of alpha-amatoxin (alpha-AMA) specific aptamers, namely, SEQ ID NO. 1 to SEQ ID NO. 18, and constructs an electrochemical and colorimetric multi-signal detection alpha-amatoxin platform based on the aptamer Apt-14 with the lowest Kd of 6.128nM, modifies the aptamer on a gold-plated glassy carbon electrode, releases the complementary strand of the aptamer by using the specific binding of the aptamer and the target, triggers HCR amplification to perform signal amplification, and the detection limit is 5ng / mL, and the effect is good. The method is simple in operation, and the enzyme-free amplification mode is more conducive to on-site detection.