The invention discloses an
oligonucleotide probe
pool for detecting a
Z chromosome and a W
chromosome of a chicken and an application of the
oligonucleotide probe
pool. The
oligonucleotide probe
pool comprises a
Z chromosome DMRT1
gene oligonucleotide probe pool: at least 500 kinds of
Z chromosome DMRT1
gene oligonucleotide probes which are reversely complementary with coding and non-coding regions in a Z
chromosome DMRT1
gene; the W
chromosome Wpkci gene oligonucleotide probe pool comprises at least 500 kinds of W chromosome Wpkci gene oligonucleotide probes which are reversely complementary with coding and non-coding regions in the W chromosome Wpkci gene, and the
fluorescence mark of the oligonucleotide probe targeting the Z chromosome DMRT1 gene is different from the
fluorescence mark of the oligonucleotide probe targeting the W chromosome Wpkci gene. The method has the remarkable advantages of
high resolution, high specificity, low cost, simplicity and convenience in preparation, high flexibility, high
throughput detection capability and the like; the practical application effect of the method is fully embodied in chicken sex identification,
chromosome abnormality detection,
genome research and poultry breeding, powerful
technical support can be provided for related fields, and the method has wide market prospects and application value.