31 results about "Z chromosome" patented technology

The invention discloses primers, a kit and a detection method for detecting the genders of cotuenix coturnix, and belongs to the technical field of molecular biology. According to the invention, specific primers are designed by using length difference of CHD1 (Chromodomain-Helicase-DNA-binding protein 1) genes of the cotuenix coturnix on Z chromosome and W chromosome; genome DNAs of the cotuenix coturnix are taken as templates for performing PCR (Polymerase Chain Reaction) amplification and dissolution curve analysis; the karyotype of female cotuenix coturnix is ZW type, a PCR product contains two fragments with different sizes, and the sizes of strips are 470bp and 638bp respectively, so that the dissolution curve has two peaks; the karyotype of male cotuenix coturnix is ZZ type, a PCR product only contains one fragment, and the size of the strip is 638bp, so that the dissolution curve has one peak; the male cotuenix coturnix and the female cotuenix coturnix can be clearly distinguished by using the difference of the dissolution curve. The detection method for the genders of the cotuenix coturnix, disclosed by the invention, has the characteristics of quickness, accuracy, economy and practicality, and is suitable for high-throughput operation.

The invention belongs to the technical field of animal breeding, in particular to a method for identifying fast feather type and slow feather type of chickens by applying double PCR. The method comprises the following steps: (1) designing two pairs of primers by utilizing a correlation sequence of a K gene locus found on a Z chromosome of a chicken according to structures of alleles of the fast and slow feather type; (2) extracting DNAs of chicken blood samples of the known fast and slow feather type to carry out a primer specificity test, and identifying the chicken to be a fast feather type or a slow feather type according to the size and the existence of an amplified DNA segment. When only one 350bp band is amplified, the chicken is judged to be a fast feather type, and when two bands of 350bp and 909bp are amplified, the chicken is judged to be a slow feather type. The invention also discloses a reliable checking method of the identifying method and applies the checking method to a breeding process of a self identification male and female matched system of the fast and slow feathers. The invention can shorten the breeding time of the self identification male and female matched system of the fast and slow feathers and save the breeding cost.

The invention relates to a gender-specific microsatellite marker for Cynoglossus semilaevis and the application of same in the identification of superfemale Cynoglossus semilaevis. The Gender-specific microsatellite marker for the Cynoglossus semilaevis is characterized in that the nucleotide sequences of a microsatellite marker are respectively SEQ ID NO: 1 and SEQ ID NO: 2; and the upstream and downstream primer sequences of a primer used for amplifying the specific microsatellite marker are respectively SEQ ID NO: 3 and SEQ ID NO: 4. The primer is used for identifying individual female or male Cynoglossus semilaevis, the amplified product segment of the superfemale Cynoglossus semilaevis is 218 bp, and the amplified product of the female Cynoglossus semilaevis is two DNA segments, the sizes of which are respectively 216-220bp and 204-208 bp, and the amplified product of the male Cynoglossus semilaevis is one DNA segment, the size of which is 204-208 bp. The invention screens the specific microsatellite markers for W and Z chromosomes of the Cynoglossus semilaevis and designs the microsatellite primer. ZW female Cynoglossus semilaevis, WW superfemale Cynoglossus semilaevis and ZZ male Cynoglossus semilaevis can be fast identified by utilizing the microsatellite marker, thereby solving the problem of hard identification of the WW superfemale Cynoglossus semilaevis and the normal ZW male Cynoglossus semilaevis during the sex control and the unisexual seed cultivation of the Cynoglossus semilaevis.

The invention discloses a target sequence suitable for transgene fixed point insertion of silkworm W-chromosome and a locus and application thereof. The nucleotide sequence of the target sequence is shown in SEQ ID NO.19 and is a specific sequence in silkworm W-chromosome, and Z-chromosome and autosome do not comprise the sequence; the target sequence inserted by a foreign gene can be expressed normally; therefore, the target sequence can be used as the fixed point insertion locus of the foreign gene in the silkworm W-chromosome so as to prepare W-chromosome chain transgenic silkworms, and has significance to identification of male and female silkworms.

The invention first provides a Cynoglossus semilaevis gender specific microsatellite marker, and comprises a microsatellite marker located in the Z chromosome, and the nucleotide sequence is SEQ ID NO:1. The invention also provides primers designed based on the above microsatellite marker, and the sequences of the upstream primer and the downstream primer are SEQ ID NO:2 and SEQ ID NO:3 respectively. The invention screens a Cynoglossus semilaevis gender specific microsatellite marker, and the Cynoglossus semilaevis gender specific microsatellite marker is subjected to SCAR conversion. Primers marked by SCAR are designed for Cynoglossus semilaevis heredity gender identification. Female, male and superfemale individuals of Cynoglossus semilaevis can be distinguished rapidly, accurately and effectively. Because the marker has characteristics that objective straps can be amplified in females and males and the objective straps can be distinguished with agarose electrophoresis, the time for accurate identification of Cynoglossus semilaevis heredity genders is shortened obviously, the on-site batch identification of Cynoglossus semilaevis heredity genders in a culturing farm of Cynoglossus semilaevis can be carried out, the detection cost is saved, and the work efficiency and accuracy of on-site detection of Cynoglossus semilaevis heredity genders in a culturing farm are raised.

The present invention provides a screening method, a kit and applications of a cynoglossus semilaevis gunther sex conversion genetic control site. The screening method comprises: carrying out full-genome molecular marking on genetic female cynoglossus semilaevis gunther to obtain a plurality of SNP loci; and carrying out full-genome association analysis on the plurality of the SNP loci by adopting whether the transgenic female fish has sex conversion as the trait to obtain the cynoglossus semilaevis gunther sex conversion genetic control site. According to the present invention, from the perspective of the genetic control for controlling the sex conversion of the female fish, the full-genome analysis is performed by using the correlation between the unique SNP loci of the transgenic female fish and the sex conversion so as to find the molecular switch for controlling the sex conversion of the genetic female fish, wherein the molecular switch is the 6676874 site nucleotide on the Z chromosome; and by using the site to control the copulation method of the male cynoglossus semilaevis gunther and the female cynoglossus semilaevis gunther, the proportion of the female cynoglossus semilaevis gunther being not subjected to sex conversion in the artificial breeding cynoglossus semilaevis gunther progeny is increased so as to improve the breeding production benefits.

The invention provides a cynoglossus semilaevis sex chromosome-linked DNA segment and application thereof. A sequence of a DNA segment on a Z chromosome is SEQ ID NO:1; a sequence of a DNA segment on a W chromosome is SEQ ID NO:2. The DNA segment is used for designing a molecular marker for identifying the genetic sex of cynoglossus semilaevis. Two Z-W chromosome homologous and different DNA segments are screened from cynoglossus semilaevis whole genome information, a primer is designed for identifying the genetic sex of the cynoglossus semilaevis, and the genetic sex of the cynoglossus semilaevis can be distinguished quickly, accurately and effectively. By adopting a method, specific purpose stripes can be amplified in male and female individuals and can be distinguished by agarose electrophoresis, the time for accurately identifying the genetic sex of the cynoglossus semilaevis is shortened, the method is suitable for identifying the genetic sex of the cynoglossus semilaevis in a simple environment of a farm, and detection time and cost are reduced.

The invention discloses a return track way arrangement method for a subway vehicle segment. The method comprises following steps: firstly, collecting the required to-be-returned vehicle information and the required to-be-returned track way information and establishing parameter sets of practical problems; secondly, selecting a proper encoding mode to configure the chromosomes and making population initialization rules so as to generate Z chromosomes to form an initial population P; then, according to the practical problems, determining an optimal target function and a constraint; and finally, determining a selection rule of individuals and a specific operation mode of hybridization and variation, calculating an adaptive value of each individual and storing the individual with the biggest adaptive value according to a fitness function and repeating the operation until reaching to a maximum evolution algebra. By use of the return track way arrangement method, train tasks can be properly selected or rejected and an optimal track way arrangement scheme can be found under a resource constraint condition; a decision support is provided for subsequent daily inspection and shunting operation; and working efficiency of the vehicle segment is greatly increased.

The invention relates to a method for rapidly and accurately identifying sturgeon sex. The method includes the steps of (1) extracting DNA of a sturgeon sample to be detected; (2) carrying out PCR amplification by using a primer F and a primer R by taking a sturgeon DNA sample as a template; (3) carrying out agarose gel electrophoresis detection on an amplification product; and (4) judging the sex of sturgeon according to an electrophoresis result, the sturgeon is female sturgeon when two bands of 573bp and 123bp appear in amplification product bands, and the sturgeon is male sturgeon when only one band of 123bp appears in the amplification products. According to the method for rapidly and accurately identifying the sturgeon sex, gene sequences with same exon sequences and different intron sequences are found in a W chromosome and a Z chromosome of the sturgeon, a specific primer is designed by utilizing the difference to carry out PCR amplification, and the sex of the sturgeon is judged through a double-positive result, so that the accuracy and the stability of sex judgment of the sturgeon are improved.

The invention discloses a novel application for PRLR and a method thereof. In the novel application, copy number variation between a candidate gene of a feathering growth gene PRLR and a reference gene PCCA is authenticated by adopting a fluorescent quantitation PCR method according to a linkage relationship between a 176,324bp tandem repeat sequence existing in a Z chromosome and chicken fast andslow feather sites; the gene type can be judged simply and visually according to the difference between Ct values after finishing of the reaction, so that chicken fast and slow feather gene type, slow feather homozygosity / heterozygosity and male and female of chickens can be simultaneously distinguished; meanwhile the experiment is easy and convenient to operate, so that the breeding time and cost of the fast and slow feather pure chicken can be greatly shortened and lowered.

The invention discloses a method for establishing an oreochromisco aureus male pure line. Purified male oreochromisco aureus ZZ and normally-used female oreochromisco aureus zw are mated, generated male oreochromisco aureus Zz and generated female oreochromisco aureus Zw through mating each account for 50%, and the oreochromisco aureus are bred to sexual maturity. The female oreochromisco aureus Zw with the Z chromosome from the purified male oreochromisco aureus are mated with gynogenesis male oreochromisco aureus ZZ again, generated fry are bred to sexual maturity, and thus a large number of pure line male oreochromisco aureus ZZ individuals are generated. By means of the method of parent selecting, ripening accelerating, gynogenesis, mating selecting and backcross, the good oreochromisco aureus male pure line can be established, and the merits of oreochromisco aureus as the male breeding parent can be effectively maintained.

The invention relates to a sex chromosome specific molecular marker of Oreochromis aureus and a genetic sex identification and monosexual fish production method based on the same. According to the invention, specific molecular markers of the Z chromosome and the W chromosome of the Oreochromis aureus are simultaneously screened for the first time; the Oreochromis aureus genetic sex identificationmethod constructed based on the molecular marker is accurate, stable and efficient and can be universally applied to different populations of the Oreochromis aureus to distinguish normal female fish ZW, superfemale fish WW, transformed female fish ZZ, transformed male fish ZW, and transformed male fish WW economically, quickly and accurately to obtain lots of superfemale fish WW. The method has the important significance in studying the sex determination and differentiation mechanisms of Oreochromis aureus and realizing sex control of Oreochromis aureus. Therefore, the large-scale production of the all-male fry is realized; the breeding yield is substantially increased; the breeding cost is lowered; and the economic benefits are increased.

The invention relates to the technical field of biological information, and discloses a method for noninvasive identification of pseudo-female Chinese softshell turtle through fluorescent quantitativePCR. The method comprises the following specific steps of setting a pair of primer groups for sexual identification of the pseudo-female Chinese softshell turtle, wherein the primer groups comprise primer pairs designed by DNA sequences of target genes 18S rRNA and Gapdh; performing fluorescent quantitative PCR data processing by adopting a 2-[delta][delta]Ct method, using the Gapdh as a reference gene, and calculating the relative copy number of the 18S rRNA in a genome; and by utilizing the fact that the DNA sequence copy number of the 18S rRNA on the W chromosome of the Chinese softshell turtle is higher than that on the Z chromosome, screening out the pseudo-female Chinese softshell turtle with the ovarian gonad and the ZZ genotype. According to the identification method, the fluorescent quantitative PCR technology is used for sexual identification of the Chinese softshell turtle, detection can be achieved only through a trace amount of blood, non-invasive collection of tail bloodof the Chinese softshell turtle has no influence on health of the Chinese softshell turtle, the method has the characteristics of being efficient, high in sensitivity, good in stability and high in specificity, the blank of pseudo-female Chinese softshell turtle identification is filled, breeding of all male softshell turtle fries is facilitated, and the breeding benefit is improved.

The invention belongs to the technical field of animal breeding, in particular to a method for identifying fast feather type and slow feather type of chickens by applying double PCR. The method comprises the following steps: (1) designing two pairs of primers by utilizing a correlation sequence of a K gene locus found on a Z chromosome of a chicken according to structures of alleles of the fast and slow feather type; (2) extracting DNAs of chicken blood samples of the known fast and slow feather type to carry out a primer specificity test, and identifying the chicken to be a fast feather typeor a slow feather type according to the size and the existence of an amplified DNA segment. When only one 350bp band is amplified, the chicken is judged to be a fast feather type, and when two bands of 350bp and 909bp are amplified, the chicken is judged to be a slow feather type. The invention also discloses a reliable checking method of the identifying method and applies the checking method to a breeding process of a self identification male and female matched system of the fast and slow feathers. The invention can shorten the breeding time of the self identification male and female matchedsystem of the fast and slow feathers and save the breeding cost.

The invention discloses a method for identifying sex of schistosoma japonicum cercariae with a multiplex PCR method and belongs to the technical field of biological detection. A pair of primers is designed for bisexual Z chromosome of schistosoma japonicum, a pair of primers is designed from a characteristic amplification area of a female sequence in W chromosome, and a multiplex PCR system is constructed and can identify male and female cercariae simultaneously. The whole process of identifying the sex of cercariae takes about 4 h with the multiplex PCR method, and the cercaria sex identification time is substantially shortened as compared with existing reports. On one hand, time for establishing a schistosoma japonicum infected animal model is saved, and on the other hand, a required experiment animal model can be established more accurately according to the sex of the cercariae.

A method for using molecular genetic marker to culture ZW-type sex-linked balance lethal line of lepidopterous pests includes searching the molecular marker on chromosome Z of target pest, using it as translocation screening marker, gamma ray radiating to induce W-Z chromosomes' translocation, screening the translocated female individual, gamma ray radiating to male anjd female pests for inducing recessive lethal mutation or intercross to generate generation F1, using its male pest to mate with normal female pest and then with translocated female pest, detecting if there is living female pest in generation F2, synchronously screening two excessive lethanl genes, and culturing sex-linked balance lethal line.

A DNA editing agent is disclosed which comprises a first nucleic acid sequence for eliciting in an inducible manner a lethal phenotype of male chick embryos in an egg of a bird and a second nucleic acid sequence for directing said nucleic acid sequence for effecting said lethal phenotype to a Z chromosome of a cell of the bird. Use of the DNA editing agent is also disclosed.

A DNA editing agent is disclosed which comprises a first nucleic acid sequence for eliciting in an inducible manner a lethal phenotype of male chick embryos in an egg of a bird and a second nucleic acid sequence for directing said nucleic acid sequence for effecting said lethal phenotype to a Z chromosome of a cell of the bird. Use of the DNA editing agent is also disclosed.

We have developed a chicken (Gallus domesticus) Z-chromosome-specific DNA library in a phage vector, by means of chromosome microisolation and microcloning. The chromosomal origin, specificity and purity was evaluated by fluorescent in situ hybridization (FISH) on chicken metaphases. Heterologous chromosome painting, using this Z-chromosome-specific probe on turkey (Meleagris gallopavo) metaphases identified its homologous Z-chromosome, under the same stringent conditions as that used in the chicken, indicating a high degree of Z-chromosome sequence homology among these two species. This chicken Z-chromosome library will facilitate the development of Z-chromosome-specific DNA markers that will be useful for genetic mapping in the domestic chicken and related avian species. The Z-chromosome-specific DNA probe will also be useful for studies pertaining to the sex chromosome evolution in avian species.

The invention relates to an accurate and simple method for determining sex for Anthropoides virgo, belonging to molecular biology technology field. It comprises following steps: augmenting the special squence on W and Z chromosome on Anthropoides virgo genetic DNA by using two units of primer combination; gel electrophoresizing with 1.5% agarose, photographic recording with Gene Genius gelatin imager, determining sex according to band type: two bands are female, while one band is male. The invention is characterized in that it determines sex according to band type, needs no extra equipment, but normal operation of PCR and agar gel electrophoresis, which is simple with strong repeatability.

The invention discloses a target sequence suitable for transgene fixed point insertion of silkworm W-chromosome and a locus and application thereof. The nucleotide sequence of the target sequence is shown in SEQ ID NO.19 and is a specific sequence in silkworm W-chromosome, and Z-chromosome and autosome do not comprise the sequence; the target sequence inserted by a foreign gene can be expressed normally; therefore, the target sequence can be used as the fixed point insertion locus of the foreign gene in the silkworm W-chromosome so as to prepare W-chromosome chain transgenic silkworms, and has significance to identification of male and female silkworms.

The invention provides a cynoglossus semilaevis sex chromosome-linked DNA segment and application thereof. A sequence of a DNA segment on a Z chromosome is SEQ ID NO:1; a sequence of a DNA segment on a W chromosome is SEQ ID NO:2. The DNA segment is used for designing a molecular marker for identifying the genetic sex of cynoglossus semilaevis. Two Z-W chromosome homologous and different DNA segments are screened from cynoglossus semilaevis whole genome information, a primer is designed for identifying the genetic sex of the cynoglossus semilaevis, and the genetic sex of the cynoglossus semilaevis can be distinguished quickly, accurately and effectively. By adopting a method, specific purpose stripes can be amplified in male and female individuals and can be distinguished by agarose electrophoresis, the time for accurately identifying the genetic sex of the cynoglossus semilaevis is shortened, the method is suitable for identifying the genetic sex of the cynoglossus semilaevis in a simple environment of a farm, and detection time and cost are reduced.

The invention provides a cynoglossus semilaevis sex reversal genetic control locus, a kit containing the same and an application of the locus. The cynoglossus semilaevis sex reversal genetic control locus includes one or more following nucleotide loci on a Z chromosome: nucleotide loci at 8564889th, 8565011st, 8565016th, 8565018th, 8565031st, 8565187th, 8565217th and 8565235th and nucleotide locibetween 8565220th and 8565221st on the Z chromosome. From the perspective of genetic control of female fish sex reversal, a new molecular switch for controlling sex reversal of genetic female fishes is found out. Any one or more newly discovered genetic loci can control the mating mode of male and female cynoglossus semilaevis, and the proportion of female fishes that do not undergo sexual reversal in the offspring of artificially bred cynoglossus semilaevis is increased, and thus the production efficiency of breeding is improved.

The invention discloses a photoelectric detection method for poultry gender. The photoelectric detection method comprises the following steps: horizontally fixing a to-be-detected egg collected by mating of a female duck with W chromosome containing GFP or a female duck with Z chromosome containing GFP and a male duck without GFP, carrying out standing for a few seconds until the interior of a blastoderm is completely static, and allowing the blastoderm to be able to freely rotate at the top part; and selecting 20 to 50 sites on an eggshell in a longitudinal axis plane perpendicular to the egg, and carrying out fluorescence measurement, wherein a correlation between a fluorescence measurement position and fluorescence intensity comprises two unique forms. The photoelectric detection methodprovided by the invention has the following beneficial effects: identification of a female embryo egg can be carried out under the condition of no damage to a breeding egg, is fast and effective andhas an accuracy rate reaching 100%.

The invention discloses a method for arranging return lanes of subway car depots. This method includes the following steps: first, collect the vehicle information and stock track information required by the algorithm, and establish a parameter set for the actual problem; second, select an appropriate encoding method to construct the chromosome and formulate population initialization rules to generate Z The chromosomes constitute the initial population P; then the optimization objective function and constraints are determined according to the actual problem; finally the individual selection rules and specific operation methods of hybridization and mutation are determined, and the fitness value of each individual is calculated according to the fitness function and the individual with the largest fitness value is evaluated. Save and repeat the operation until the maximum number of evolutionary generations. This invention can make reasonable choices about train tasks and find the best track arrangement plan under resource constraints, provide decision support for subsequent daily inspections and shunting operations, and greatly improve the efficiency of depot operations.

The invention first provides a Cynoglossus semilaevis gender specific microsatellite marker, and comprises a microsatellite marker located in the Z chromosome, and the nucleotide sequence is SEQ ID NO:1. The invention also provides primers designed based on the above microsatellite marker, and the sequences of the upstream primer and the downstream primer are SEQ ID NO:2 and SEQ ID NO:3 respectively. The invention screens a Cynoglossus semilaevis gender specific microsatellite marker, and the Cynoglossus semilaevis gender specific microsatellite marker is subjected to SCAR conversion. Primers marked by SCAR are designed for Cynoglossus semilaevis heredity gender identification. Female, male and superfemale individuals of Cynoglossus semilaevis can be distinguished rapidly, accurately and effectively. Because the marker has characteristics that objective straps can be amplified in females and males and the objective straps can be distinguished with agarose electrophoresis, the time for accurate identification of Cynoglossus semilaevis heredity genders is shortened obviously, the on-site batch identification of Cynoglossus semilaevis heredity genders in a culturing farm of Cynoglossus semilaevis can be carried out, the detection cost is saved, and the work efficiency and accuracy of on-site detection of Cynoglossus semilaevis heredity genders in a culturing farm are raised.

The present invention provides a screening method, a kit and applications of a cynoglossus semilaevis gunther sex conversion genetic control site. The screening method comprises: carrying out full-genome molecular marking on genetic female cynoglossus semilaevis gunther to obtain a plurality of SNP loci; and carrying out full-genome association analysis on the plurality of the SNP loci by adopting whether the transgenic female fish has sex conversion as the trait to obtain the cynoglossus semilaevis gunther sex conversion genetic control site. According to the present invention, from the perspective of the genetic control for controlling the sex conversion of the female fish, the full-genome analysis is performed by using the correlation between the unique SNP loci of the transgenic female fish and the sex conversion so as to find the molecular switch for controlling the sex conversion of the genetic female fish, wherein the molecular switch is the 6676874 site nucleotide on the Z chromosome; and by using the site to control the copulation method of the male cynoglossus semilaevis gunther and the female cynoglossus semilaevis gunther, the proportion of the female cynoglossus semilaevis gunther being not subjected to sex conversion in the artificial breeding cynoglossus semilaevis gunther progeny is increased so as to improve the breeding production benefits.

The invention provides a practical silkworm variety cultivation method capable of controlling the male ratio. In the method, a sex-limited ant color system under the control of an sch mutant gene carried on Z chromosome of silkworm and a +sch gene translocated on a W chromosome is taken as a donor, and a Z<sch> gene and a W<+sch> gene of the donor can transferred onto a receptor through hybridization in which an ordinary ant color practical silkworm variety is taken as the receptor. In the method, the genetic regularity of key genes (namely, the Z<sch> gene and the W<+sch> gene) for controlling the male ratio are organically combined with a classical silkworm breeding method, thereby forming a practical silkworm variety method capable of controlling the male ratio in rapid breeding is constructed.