Method for cultivating ZW type lepidopteraus pest linkage balanced lethal system using molecule genetic label

A molecular genetic marker, balanced lethal technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. Cultivate problems such as difficulties, and achieve the effect of avoiding ineffective labor, involving a wide range of areas and high stability

Inactive Publication Date: 2005-01-12
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

Many pests cannot be reared artificially, and there is no distinction between populations and strains. For such species, it is very difficult to use phenotypic markers to cultivate sex-linked balance lethal strains, which is why the sex-linked balance lethal technology has not been used so far. The main reason for pest control

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  • Method for cultivating ZW type lepidopteraus pest linkage balanced lethal system using molecule genetic label
  • Method for cultivating ZW type lepidopteraus pest linkage balanced lethal system using molecule genetic label
  • Method for cultivating ZW type lepidopteraus pest linkage balanced lethal system using molecule genetic label

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Embodiment Construction

[0026] The invention mainly uses molecular genetic markers combined with comparative quantitative PCR technology to cultivate lethal lepidopteran pests in linkage equilibrium, and can be used for genetic control of lepidopteran pests. Specifically, using the gene (such as A gene) located on the autosome as a reference gene, using comparative quantitative PCR technology to find molecular markers (such as K gene) on the Z chromosome of the target pest, the molecular markers on the Z chromosome and the autosome The ratio of genes (K / A) differs between males and females, K / A=1 / 2 in females and K / A=2 / 2 in males; this difference can be used to determine the target pest Z Molecular markers on chromosomes. Translocation of the Z chromosome segment induced by γ-ray irradiation to the W chromosome, and using molecular markers on the Z chromosome (such as the K gene) as translocation screening markers, using comparative quantitative PCR technology and multicolor fluorescence in situ hybr...

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Abstract

A method for using molecular genetic marker to culture ZW-type sex-linked balance lethal line of lepidopterous pests includes searching the molecular marker on chromosome Z of target pest, using it as translocation screening marker, gamma ray radiating to induce W-Z chromosomes' translocation, screening the translocated female individual, gamma ray radiating to male anjd female pests for inducing recessive lethal mutation or intercross to generate generation F1, using its male pest to mate with normal female pest and then with translocated female pest, detecting if there is living female pest in generation F2, synchronously screening two excessive lethanl genes, and culturing sex-linked balance lethal line.

Description

Technical field [0001] The invention relates to the field of genetic breeding and genetic control of ZW-type lepidopteran pests, in particular to a method for cultivating ZW-type lepidopteran pest sex linkage equilibrium lethal lines with molecular genetic markers. Background technique [0002] For the prevention and control of Lepidoptera pests, chemical insecticides, radiation sterility or biological control are mostly used at present. Chemical pesticides have made great achievements in the field of crop pest control, but for a long time, due to the extensive use of highly toxic chemical pesticides, the three "R" problems (i.e. the re-emergence of secondary pests, the resistance of pests) sex and pesticide residues), and cause serious environmental pollution, destroying the ecological balance of nature. [0003] Pest radiation sterility control technology is a control method that artificially raises target pests, releases them in the wild after irradiation, and hybridizes...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033C12Q1/68
Inventor 孟智启牛宝龙翁宏飚沈卫锋顾伟平夏大荣
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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