3 results about "Protein translocation" patented technology

Systems and polypeptide complexes are provided that combine scaffold protein modules with a variety of effector modules, enabling comprehensive and versatile control at the protein, RNA, and DNA levels. In addition, uses of them in the modulation of gene expression and potential uses in the treatment of disease are provided.

Methods of translocating an analyte through a nanopore and corresponding systems are described. An example method comprises translocating an analyte through a nanopore in a structure defining an internal cavity and a barrier dividing the interior cavity into a first chamber and a second chamber. The barrier comprises a channel structure defining a nanopore therethrough fluidically coupling the first and second chambers. The first chamber comprises the analyte and the method comprises modifying side groups of the analyte. Said modifying changes at least one of an electrical a chemical property of the analyte. The method further comprises applying a voltage gradient between the first and second chambers via the nanopore. Applying the voltage causes a translocation of the analyte through the nanopore from the first chamber and the second chamber. Embodiments described herein can be helpful for enabling translocation or ensuring smooth translocation of an analyte through a nanopore.

The present invention relates to a composition for inhibiting protein translocation in cells harbouring a target nucleic acid (NA) molecule such as a ribonucleic acid (RNA) molecule, the composition comprising a peptide construct, comprising a protein secretion signal amino-acid sequence ("SP sequence"), and a nucleic acid mimic (NAM) sequence such as a peptide nucleic acid (PNA) sequence comprising 5 to 30 consecutive bases complementary to a sequence of the target NA molecule; and a delivery vehicle for delivering the peptide construct to cells. The present invention further relates to a method for inhibiting protein translocation in cells harbouring a target NA molecule, comprising the step of delivering to the cells a peptide construct, comprising an SP sequence, and a NAM sequence such as a PNA sequence comprising 5 to 30 consecutive bases complementary to a sequence of the target NA molecule. The present invention further relates to uses of such compositions and methods for therapy of the human or animal body, plant treatment and pest control, among other uses.