426 results about "Pseudomonas tolaasii" patented technology

The present invention mainly provides a fusion antigen specific for a target cell comprising a ligand moiety which is capable of reacting, recognizing or binding to the receptors on the target cell, a Pseudomonas exotoxin A translocation domain II, an antigenic moiety, and a carboxyl terminal moiety which permits combination of the fusion antigen to the endoplasmic reticulum (ER) membrane of the target cell. A method of immunizing an animal using the fusion antigen is also provided.

Cosmetic or dermopharmaceutical compositions containing Pseudoalteromonas ferment extract for the treatment and / or care of the skin, scalp and / or nails, preferably to improve the barrier function of the skin, scalp and / or nails, and specifically for the treatment and / or care of those conditions, disorders and / or pathologies of the skin, scalp and / or nails associated with an alteration in the keratinization process.

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu / mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu / mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu / mL(or cfu / g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

Provided herein are systems for treating a subject with a pulmonary infection, for example, a nontuberculous mycobacterial pulmonary infection, a Burkholderia pulmonary infection, a pulmonary infection associated with bronchiectasis, or a Pseudomonas pulmonary infection. The system includes a pharmaceutical formulation comprising a liposomal aminoglycoside dispersion, and the lipid component of the liposomes consist essentially of electrically neutral lipids. The system also includes a nebulizer which generates an aerosol of the pharmaceutical formulation at a rate greater than about 0.53 gram per minute. The aerosol is delivered to the subject via inhalation for the treatment of the pulmonary infection.

The invention relates to an agricultural fertilizer, in particular to a microbial compound fertilizer and application thereof. The microbial compound fertilizer specifically consists of a zymophyte agent and a compound fertilizer, wherein each gram of the compound fertilizer comprises not less than 0.2*108 bacterial cells; the compound fertilizer comprises the following components in percentage by weight: 5 to 15 percent of farmyard manure, 10 to 30 percent of humic acid, 5 to 8 percent of grass carbonic acid, 15 to 30 percent of nutrient and the balance of boric sludge; and the zymophyte agent is obtained by fermenting silicate bacteria, azotobacter chroococcum, actinomycetes, saccharomyces cerevisiae and rhodopseudomonas sphaeroides, wherein the silicate bacteria, the azotobacter chroococcum, the actinomycetes, the saccharomyces cerevisiae and the rhodopseudomonas sphaeroides are mixed together in a weight fraction of 1:1:1:1:1. The microbial compound fertilizer acting on a big field can increase the yield of crops by 20 to 30 percent, and simultaneously can obviously increase the percentage of fertile fruits, reduce fruit dropping and overgrow the fruits; besides, the maturation stage is shortened by 5 to 7 days, the yield-increasing effect is obvious, and simultaneously, the microbial compound fertilizer can obviously improve the appearance, the mouthfeel, the color and luster and the like of products, improve the quality by 1 to 2 grades, and prolong the product freshness date.

The invention provides a special bacterial manure for peanut planting, which comprises the following components in parts by weight: 5-25 parts of a compound microbial agent, 30-65 parts of dreg composite powder, 5-20 parts of starch and 10-25 parts of bentonite, wherein the compound microbial agent is composed of bacillus subtilis agent, a rhodopseudomonas palustris agent, a trichoderma harzianum agent and a paecilomyces lilacinus agent; the mass ratio of the bacillus subtilis agent to the rhodopseudomonas palustris agent to the trichoderma harzianum agent to the paecilomyces lilacinus agent is 5:2:1:2, and the total effective viable count of the compound microbial agent is10 billion cfu / g. The bacterial manure is mainly used as a base fertilizer, and can change the microenvironment around the roots of peanuts to prevent various plant diseases and insect pests while promoting nutrient absorption of the peanuts, so that the yield and quality of the peanuts are improved.

The invention discloses a microorganism preparation for improving cultivation water. The microorganism preparation is characterized in that raw materials include 20-40% of bacillus subtilis, 20-40% of bacillus amyloliquefaciens, 20-50% of saccharomyces boulardii and 5-10% of rhodopseudom onas palustrisand. A preparation method comprises the steps of: respectively inoculating the bacillus subtilis, the bacillus amyloliquefaciens, the saccharomyces boulardii and the rhodopseudom onas palustrisand into corresponding culture media to obtain seed culture solutions; inoculating the seed culture solutions into the culture media according to proportion in sequence for fermentation, and performing high-speed centrifugation to obtain high-concentration bacilli liquid; transferring the bacilli liquid into a matrix, mixing, granulating, drying and crushing. After the microorganism preparation disclosed by the invention is used, the cultivation water becomes clear, water change amount is reduced remarkably, water resources are saved, and pollution caused by outward emission is reduced. Due to improvement of the quality of a water environment, drugs used for aquaculture products are reduced, antibiotic pollution is reduced, the food safety requirement is met, and product quality is ensured.

The invention relates to an agricultural compound biofertilizer, in particular to a compound biofertilizer capable of improving the physical and chemical properties of soil and improving the yield and quality of agricultural products and a preparation method thereof. The fertilizer contains the following materials in percentage by weight: 30-40% of organic substance, 15-25% of plant straw, 14-15% of mixed biological fermentation bacterium liquid and 30-31% of inorganic nutrient, wherein the biological bacteria used in the invention comprise Rhodopseudomonas palustris, microzyme, azotobacter, phosphorus-solubilizing Bacillus megaterium and silicate bacteria; the organic substance components are humic acid, worm excretion and bean cakes; and the inorganic nutrient comprises N, P and K nutrient. The compound biofertilizer can be applied as basal fertilizer or additional fertilizer, and is applicable to all crops. By using the compound biofertilizer, the yield increase amplitude can be 8-10%.

The invention provides mutated Pseudomonas exotoxins (PE) that have reduced immunogenicity compared to PEs containing the native sequence. The PEs of the invention have one or more individual mutations of positions of the native sequence of PE that reduce antibody binding to one or more PE epitopes. Nucleic acids encoding the mutated PEs, chimeric molecules comprising them, compositions comprising the chimeric molecules and methods of using them, are also provided.

A method of amplification culture of microorganism for waste water treatment. The waste water comprises heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and / or lignin. The method comprises the following steps: adding waste water containing heterocyclic compounds, polycyclic aromatic hydrocarbons, cellulose and / or lignin into an acclimation reactor; adding a nutritional agent, acomplex bacterial agent, an enzyme preparation, a citric acid and riboflavin on a basis of concentrations in the waste water; performing aeration of the waste water, adjusting the liquid level and temperature of the acclimation reactor, holding for a period of time to allow the microorganism in the complex bacterial agent to reach a logarithmic growth phase, wherein on a basis of concentrations in the waste water, the complex bacterial agent comprises pseudomonas spp. microorganism, bacillus sp. microorganism, and white rot fungi, and the enzyme preparation comprises protease, amylase, cellulose and lipase. The invention also provides a method of waste water treatment by using the microorganism to be amplified and cultured.

The invention discloses a composite microbial agent and a preparation method and application thereof. The composite microbial agent includes a pseudomonas preparation, an acinetobacter preparation, a bacillus amyloliquefaciens preparation, a bacillus subtillis preparation, a bacillus licheniformis preparation, a pediococcus acidilactici preparation, a rhodobacter sphaeroides preparation, an alkali-lake dietzia preparation and a rhodopseudomonas palustris preparation. The preparation method includes the following steps that 1, composite microbial agent components are prepared; 2, the composite microbial agent components are mixed in proportion to obtain the composite microbial agent. The composite microbial agent can simultaneously decompose different organic matter, degrade amino nitrogen and nitro nitrogen in water, reduce phosphorus concentration in the water body and inhibit the growth of algae. The composite microbial agent is used for urban-rural sewage in-situ remediation, has the advantages of being high in efficiency, quick to take effect, free of no secondary pollution, simple in operation and the like, and large-scale application and popularization are facilitated.

The invention relates to a microorganism composite bacterial agent applicable to the restoration of saline alkali soil polluted by petroleum, and a preparation method thereof, and belongs to the technical field of environment protection. The bacterial agent mainly comprises the following 3 components: composite microorganism bacterial liquid, nutrients, and a surfactant. The composite microorganism bacterial liquid is obtained by performing liquid culture of 3 petroleum hydrocarbon degrading bacteria of bacillus megaterium P9 strains (with a preservation number of CGMCC No. 4270), pseudomonas sp. P4 strains (with a preservation number of CGMCC No. 4269), and achromobacter xylosoxidans P2 strains (with a preservation number of CGMCC No. 4268) to obtain microorganism bacterial strains, and mixing the strains with the nutrients. The bacterial strains are preserved at China microorganism strain preservation management committee general microbiological center. The composite microorganism bacterial liquid, the nutrients and the surfactant are mixed according to a ratio of 30-200:1-2:0.2-0.5 so as to obtain the microorganism composite bacterial agent. The microorganism composite bacterial agent prepared by the invention has high removing efficiency for petroleum pollution in saline alkali soil, can both reduce the pH value of the saline alkali soil, and improve soil physical and chemical properties.

According to the present invention a new isolate of a Pseudomonas spp, DSM 21663, has been shown to possess unique properties. This Pseudomonas is a plant growth-promoting rhizobacterium (PGPR). Among its modes of action involved in plant growth-promotion are anti-biotic production (2,4-diacetylphloroglucinol, (DAPG); pyrrolnitrin, PRN and others), indole-3-acetic acid (IAA) production and phosphate solubilization, and production of unique volatiles. The strain is fluorescent, oxidase-positive, and has the ability to suppress soil-borne root and foliar pathogens of both fungal and bacterial origin.

The invention relates to a kitchen garbage degrading composite microbial inoculum and a preparation method and application thereof. The kitchen garbage degrading composite microbial inoculum is prepared by mixing a mixed inoculum of bacillus amyloliquefaciens, radiation-resistant methylobacterium, dispersed pantoea, pseudomonas oryzihabitans, citrobacter freundii and staphylococcus cohnii and a carrier, wherein the mass of the mixed inoculum accounts for 15-25% of the total mass of the kitchen garbage-degrading composite microbial inoculum; the mass ratio of the bacillus amyloliquefaciens, the radiation-resistant methylobacterium, the dispersed pantoea, the pseudomonas oryzihabitans, the citrobacter freundii and the staphylococcus cohnii is (1.5-3):(1-1.5):(1-1.5): (1-1.5):(1-1.5):(1-1.5); the kitchen garbage-degrading composite microbial inoculum is used for degrading kitchen garbage. Compared with the prior art, the kitchen garbage degrading composite microbial inoculum has the advantages as follows: the kitchen garbage degrading composite microbial inoculum can effectively degrade common vegetables, grains, fish, poultry meat and other kitchen garbage, has the degradation rate being above 80%, is high in degradation speed rate and small in odor, does not produce pollutants or poisonous substances, and is low in cost and stable in performance.

The invention belongs to the technical field of biology and relates to a method for producing a microecological foliar fertilizer by utilizing probiotics and microalgae. The method comprises the following steps of: firstly, culturing to obtain liquid culture prepared by bacillus, lactic acid bacteria and rhodopseudomonas palustris with the cell concentration being 10 billion / ml and saccharomycetes and chlorella with the cell concentration being 2 billion / ml respectively, then preparing liquid culture with four types of probiotics and one type of chlorella, and mixing the liquid cultures according to respective volume ratio to obtain the liquid microecological foliar fertilizer with the total cell concentration being more than 5 billion / ml. The method has the advantages that the used strains and chlorella are all probiotics which can be eaten by human, and are safe and non-toxic; the microecological foliar fertilizer integrates the advantages of promoting the plant growth of the probiotics, the chlorella and metabolites thereof; and the microecological foliar fertilizer is diluted with water by 100 times every 10 days and is sprayed on plant leaves, and the plant yield is increasedby more than 30% in 60 days. The method has an important application value in aspect of improving the yield and the quality of crops such as grain and the like.

The invention relates to Pseudomonas chlororaphis with wide-spectrum antimicrobial activity and application of Pseudomonas chlororaphis to prevention and treatment of diseases attacking fruit trees and field crops, belonging to the field of biotechnology. The Pseudomonas chlororaphis with wide-spectrum antimicrobial activity is classified and named as Pseudomonas chlororaphis YTBTa14, with an accession number of CCTCC No. M2016099, and the 16SrDNA sequence of the Pseudomonas chlororaphis is as shown in a sequence table in the specification. The thallus and fermentation broth of the Pseudomonas chlororaphis with wide-spectrum antimicrobial activity in the invention both have good wide-spectrum antimicrobial activity to fungal diseases attacking fruit trees, have long-lasting and stable antimicrobial effect, show great potential in development into novel high-efficiency wide-spectrum bio-control fungicides, and provide a novel approach for application of microbes to biological control.

The invention discloses a probiotics microbial agent for regulating livestock intestinal flora and a preparation method thereof. The probiotics microbial agent is prepared by using candida utilis, streptococcis faecium, bacillus subtilis, bacillus natto, lactobacillus plantarum and rhodopseudomonas palustris as raw materials, performing the three-stage liquid fermentation, then uniformly mixing the enlarged cultivated fermenting bacteria with a feed wheat bran, performing the solid fermentation, drying at low temperature to obtain the probiotics agent for regulating the livestock intestinal flora structure. The agent can be used for regulating the livestock intestinal flora structure, promoting the growth of the probiotics, inhibiting the growth of the harmful bacteria, substituting the antibiotics, improving the feed utilization rate, obviously reducing the odor of the excrement of the livestock and improving the ecological environment of aquiculture and round environment, and is efficient, non-toxic and no residue. The probiotics microbial agent can be widely applied to a livestock feed additive so as to prevent the livestock intestinal disease and respiratory disease.

The invention discloses a microbial preparation for improving an aquatic product culture environment and a preparation method thereof. A strain with optimal nitrite degradation effects is screened and separated from a mud sample in Tongan and Haicang ports of Xiamen city of the Fujian province, another strain with optimal nitrite degradation effects is screened and separated from a mud sample in the intertidal zone of Ningde city of the Fujian province, and the two strains respectively are Rhodopseudomonas palustris PSB20 and Bacillus subtilis ND04. The microbial preparation prepared from the two strains can degrade ammonia nitrogen and nitrous acid in an aquatic product culture environment, has good decomposition effects on residual bait organic matters, has a certain effect of inhibiting pathogenic vibrio bacterium growth in culture water, and has a good production application prospect. The microbial preparation has the characteristic of simple preparation processes.

The present invention discloses a biological deodorant. The biological deodorant is characterized in that the biological deodorant comprises molasses, water and a composite strain, wherein the composite strain comprises Bacillus sp, Actinomyces sp, Saccharomyces sp, Lactobacillus sp and Rhodobacter sp, a weight ratio of the bacteria is 2:2:1:3:2, the bacteria are inoculated into a medium prepared by dissolving molasses in water, and the sugar degree of the molasses is more than or equal to 5%. Compared to the biological deodorant in the prior art, the biological deodorant of the present invention has the following advantages that: various foul gases generated by a large amount of livestock and poultry manures due to breeding can be completely removed so as to improve environmental conditions of farms and farmers, reduce surrounding environmental pollution, and ensure lives and productions of local residents; a good foundation is established for comprehensive recovery and utilization, and harmless treatment of livestock and poultry manures, and strong technical supports are provided for improvement of circulation of agricultural economy.

The invention relates to in-situ restoration of pesticide contaminated soil, in particular to a preparation method of microbe immobilized particles for restoring pesticide contaminated soil. Porous carbon is used as a main carrier material, and cellulose, chitosan and sodium alginate are used as auxiliary carriers to realize the purpose of immobilizing the microbes; and the obtained microbe immobilized particles are used for in-situ restoration of the pesticide contaminated soil. The carrier material used in the invention has cheap price, low cost, wide sources, good air permeability and no toxicity to organisms, and can not cause secondary pollution to the soil. The carrier and the immobilizing process thereof can be used for immobilizing multi-bacterium microbes, such as pseudomonas, bacillus, zoogloea, micrococcus and the like, and have certain broad-spectrum applicability; and the bacillus can reach more than 10 billions / gram. Compared with the prior immobilizing method, the invention is characterized by directly sowing the immobilized cell particles into the soil without destroying the original soil structure.

The invention discloses a plant microecological preparation for enhancing the immunity of an animal and a preparation method of the plant microecological preparation, and belongs to the field of feed additives. The plant microecological preparation is prepared by the following steps: mixing crushed materials or extracted materials of radix pseudostellariae, liquorice, rhizoma atractylodis macrocephalae, honeysuckle, astragalus membranaceus, eucommia ulmoides, great burdock achene, dandelion and herba epimedii, adding a culture solution to prepare a culture medium, adding a total bacterium liquid consisting of two or more than two of lactobacillus plantarum, enterococcus faecalis, enterococcus faecium, enterococcus faecium, pediococcus acidilactici, pediococcus pentosaceus, rhodopseudomonas palustris, bacillus subtilis and lactobacillus fermenti, fermenting and drying. The plant microecological preparation disclosed by the invention has the advantages that the immunity of the animal can be obviously improved, the production performance can be improved and the diarrhea rate can be reduced.

A process for removing the anti-nutrition factor from soybean dregs by fermenting method includes providing raw soybean dregs, adding water, inoculating yeast, lactobacillus, Bacillus subtilis, etc, fermenting at 30-55 deg.C, and treating the finished product to obtain the feed of young animals.

The invention discloses a microbial agent for purifying sanitary sewage and a preparation method of the microbial agent. Active ingredients of the microbial agent is formed by mixing the following raw material bacteria including thiobacillus denitrificans, bacillus subtillis, bacillus licheniformis, bacillus polymyxa, lactic acid bacteria, nocardia amarae, denitrifying bacteria, rhodopseudomonas palustris, thiophilic red pseudomonas, rhodospirillum, saccharomycetes and propionibacterium. By means of the microbial agent, cellulose, saccharides, fat protein, pathogenic bacteria, viruses and parasitic ovum in sanitary sewage and pollutants, such as inorganic salt which is high in the contents of nitrogen, sulphur and phosphorus, the BOD and COD removal effect is guaranteed, meanwhile good bactericidal ability is achieved, high inhibiting ability is achieved especially for pathogenic bacteria, and the effect of treating sanitary sewage is remarkable.

The invention discloses a preparation method of charcoal loaded PSB passivator, belongs to the technical field of soil restoration and further provides a method for restoring Pb polluted soil through the charcoal loaded PSB passivator. Green needle pseudomonades are adopted and immobilized; with charcoal as a carrier, the green needle pseudomonades cultured for the logarithmic phase are inoculated into a fermentation tank, charcoal is added for fermentation culture, then, immobilization is conducted, and the charcoal loaded PSB passivator is obtained. The preparation method is simple, easy to operation and suitable for large-scale production, the restoration capability of the charcoal loaded PSB passivator to soil is high, and the removing rate of lead is high.

The invention belongs to preparation for microorganism-decomposing agents, and in particular discloses a method for preparing a microorganism-decomposing agent used for producing a biological organic fertilizer by livestock and poultry manure fermentation. The method comprises the following steps of: respectively inoculating Saccharomyces cerevisiae AS1.2414 and Saccharomyces cerevisiae AS1.1854 into a culture medium which contains a carbon source, a nitrogen source and nutrient substances for fermentation; inoculating Rhodopseudomonas palustris AS 1.2349 into a culture medium which contains nutrient substances for fermentation; and mixing saccharomyces cerevisiae fermentation liquor, rhodopseudomonas palustris fermentation liquor and rhodopseudomonas palustris fermentation liquor according to a volume ratio of 50:25:25, and taking a mixture of rice bran and bran as a carrier to adsorb the mixed fermentation liquor so as to prepare a solid decomposing agent. The method solves the problems of the quality and the speed of composting influenced by the relatively low composting speed in the composting process of the prior art, and has the advantages of effectively reducing the composting time, improving the compost maturity and the production efficiency, and the like.

The invention discloses a preparation method of a composite microbial inoculant for degrading aflatoxin B1 and a composite microbial inoculant prepared by the method. The method comprises the following steps: respectively culturing pseudomonad, Flavobacterium, Rhodococcus, Stenotrophomonas and Bacillus to obtain a pseudomonad solution, a Flavobacterium solution, a Rhodococcus solution, a Stenotrophomonas solution and a Bacillus solution; mixing the pseudomonad solution, Flavobacterium solution, Rhodococcus solution, Stenotrophomonas solution and Bacillus solution to obtain a composite bacterium solution; and putting the composite bacterium solution into a composite bacterium culture medium, and carrying out mixed culture fermentation to obtain the composite microbial inoculant for degrading aflatoxin B1. The composite microbial inoculant can efficiently degrade aflatoxin B1, and has the advantages of low cost and no secondary pollution.

Belonging to the field of microbial agents and fertilizers, the invention specifically relates to an Enteromorpha microbial agent suitable for the demands of planting, livestock and poultry, aquaculture, sewage treatment and other fields, and further discloses a preparation method of the microbial agent. The Enteromorpha microbial agent provided by the invention employs Enteromorpha to prepare a slurry, and is compounded with Streptomyces microflavus, Lactobacillus casei, bacillus subtilis, rhodopseudomonas palustris, Pichia membranaefaciens and other main beneficial microbial floras, and has the multiple functions of effectively improving agricultural product quality, effectively lowering the cost of animal and plant growth period, increasing agricultural product output and the like. The microbial agent also has the functions of purifying water, removing hydrogen sulfide and other odors from sewage, garbage and livestock raising housings, and improving the environment, etc.

The invention relates to compound microbial fertilizer, which is prepared from a fermented mushroom residue, humic acid, well rotten chicken manure and a compound microbial agent. The compound microbial agent is prepared from rhodospirillum rubrum and 3 to 6 kinds of bacillus megaterium, bacillus subtilis, bacillus licheniformis, bacillus cereus, bacillus latersprorus and rhodopseudomonas palustris. The compound microbial fertilizer provided by the invention can be used for promoting the photosynthesis of a plant and optimizing the composition of nutrient substances in a product; a microbe can be used for dissolving phosphorus and dissolving potassium, improving the fertility of soil, improving the microbial activity of the soil, promoting the nutrient accumulation of a crop and ameliorating product quality. The fermented mushroom residue, the humic acid and the well rotten chicken manure are added, and not only can be used for providing fertilizer for the growth of the microbe, but also can be used for ameliorating the physical and chemical properties of the soil, promoting the formation of the granular structure of the soil, enhancing the air-permeation, water-holding and fertility-accumulation capacity of the soil, improving the utilization rate of the fertilizer and protecting an environment.

A method of preventing or treating infection by a range of fungal pathogens in an animal or plant or stored plant product comprising administering an effective amount of a sugar acid, in particular mannonic acid, gluconic acid or galacturonic. The sugar acid can be delivered by a biocontrol that can manufacture the sugar acid. An exemplified biocontrol is Pseudomonas strain AN5, which is characterized by the capacity to convert aldose to sugar acid. Also disclosed are nucleotide sequences from enzymes in the Pseudomonas PQQ enzyme pathway which may be used to transform microorganisms and plants so that they produce sugar acids at levels sufficient to control the growth or viability of pathogens.