Enteromorpha microbial agent and preparation method thereof

A technology for microbial inoculants and prolifera, applied in the field of microbial inoculants and fertilizers, can solve problems such as inability to comprehensively apply, and achieve the effects of improving physical and chemical properties, improving the environment, and enhancing disease prevention and disease resistance.

Active Publication Date: 2015-10-07
日照海韵环保生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Similarly, this foliar fertilizer is also made for the development of soil crops, and still cannot be widely and comprehensively applied to the needs of crop planting, livestock and poultry and aquaculture.

Method used

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  • Enteromorpha microbial agent and preparation method thereof
  • Enteromorpha microbial agent and preparation method thereof
  • Enteromorpha microbial agent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Enteromorpha microbial bacterial agent described in the present embodiment is prepared by the following steps from the following components:

[0045] S1, taking fresh Enteromorpha and subjecting it to high-temperature sterilization above 100°C, sterilizing, soaking, crushing, and grinding to make Enteromorpha slurry to obtain Enteromorpha slurry;

[0046] S2, respectively insert the preserved Streptomyces flavinus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranosus into their appropriate medium for aseptic culture, and follow the cell culture Streptomyces flavus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranosa in a weight ratio of 2:3:1:3:1 and mixed to obtain biological strains ;in,

[0047] The formula of the Streptomyces luteus culture solution is: soluble starch 2.0g, potassium nitrate 0.1g, dipotassium hydrogen phosphate 0.05g, sodium chloride 0.05g, magnesium sulfate 0.05g, ferrous su...

Embodiment 2

[0056] Enteromorpha microbial bacterial agent described in the present embodiment is prepared by the following steps from the following components:

[0057] S1, taking fresh Enteromorpha and subjecting it to high-temperature sterilization above 100°C, sterilizing, soaking, crushing, and grinding to make Enteromorpha slurry to obtain Enteromorpha slurry;

[0058] S2, respectively insert the preserved Streptomyces flavinus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranosus into their appropriate medium for aseptic culture, and follow the cell culture Streptomyces flavus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranosa in a weight ratio of 4:1:3:1:3 and mixed to obtain biological strains ; Wherein, the culture medium that each strain uses is all identical with embodiment 1;

[0059] S3, get the enteromorpha slurry 35kg that step S1 obtains, the biological strain 3kg that step S2 obtains, and sucrose...

Embodiment 3

[0063] Enteromorpha microbial bacterial agent described in the present embodiment is prepared by the following steps from the following components:

[0064] S1, taking fresh Enteromorpha and subjecting it to high-temperature sterilization above 100°C, sterilizing, soaking, crushing, and grinding to make Enteromorpha slurry to obtain Enteromorpha slurry;

[0065] S2, respectively insert the preserved Streptomyces flavinus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranosus into their appropriate medium for aseptic culture, and follow the cell culture Streptomyces flavus, Lactobacillus casei, Bacillus subtilis, Rhodopseudomonas palustris and Pichia membranosa in a weight ratio of 3:1.5:2:2:1.5 and mixed to obtain biological strains ; Wherein, the culture medium that each strain uses is all identical with embodiment 1;

[0066] S3, get the enteromorpha slurry 32kg that step S1 obtains, the biological strain 4kg that step S2 obtains, and suc...

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Abstract

Belonging to the field of microbial agents and fertilizers, the invention specifically relates to an Enteromorpha microbial agent suitable for the demands of planting, livestock and poultry, aquaculture, sewage treatment and other fields, and further discloses a preparation method of the microbial agent. The Enteromorpha microbial agent provided by the invention employs Enteromorpha to prepare a slurry, and is compounded with Streptomyces microflavus, Lactobacillus casei, bacillus subtilis, rhodopseudomonas palustris, Pichia membranaefaciens and other main beneficial microbial floras, and has the multiple functions of effectively improving agricultural product quality, effectively lowering the cost of animal and plant growth period, increasing agricultural product output and the like. The microbial agent also has the functions of purifying water, removing hydrogen sulfide and other odors from sewage, garbage and livestock raising housings, and improving the environment, etc.

Description

technical field [0001] The invention belongs to the field of microbial bacterial agents and fertilizers, and specifically relates to an enteromorpha microbial bacterial agent suitable for the needs of fields such as planting, livestock and poultry, aquaculture, and sewage treatment, and further discloses a preparation method thereof. Background technique [0002] In the current economic development, crop planting, livestock and poultry and aquaculture have formed a huge industrial scale in our country and occupy an important position in the national economy. However, in order to pursue high returns, people use a large amount of chemical fertilizers and pesticides in the planting process, and add antibiotics, hormones, and preservatives to livestock, poultry, and aquatic products during breeding, resulting in a large amount of nitrite and pesticides in grains and vegetables. Residues, the production of drug-resistant strains and antibiotics, hormones residues in livestock and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12R1/465C12R1/245C12R1/125C12R1/38C12R1/84
CPCC12N1/16C12N1/20
Inventor 刘丽君李作梅战培林李辉战意和桑俊玲成莹
Owner 日照海韵环保生物科技发展有限公司
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