Facial cleanser with double cleaning effects and preparation method thereof

Abstract

The invention relates to the technical field of biology, in particular to a facial cleanser with double cleaning effects and a preparation method of the facial cleanser. According to the invention, the soap base and the amino acid surfactant are compounded to form a dual cleaning system, so that the problems that the skin is dry and sensitive due to the fact that a soap-base powerful cleaning agent can damage the skin barrier after being used for a long time, and the cleaning power is insufficient when the amino acid surfactant is independently used are solved; citric acid is specifically selected as the pH regulator and can be matched with potassium cocoyl glycinate, so that the cleaning capability of the facial cleanser is further enhanced; according to the invention, glycerin, hydrolyzed sodium hyaluronate, aloe extract and other components are added at the same time, so that good moisturizing and moisturizing effects are provided for the facial cleanser; the rose extract and the aloe extract which are fermented and modified by the lactobacillus and the lactobacillus plantarum are used, so that the micro-ecological balance of the skin can be adjusted, the reproduction of harmful bacteria is inhibited, and a good microbial environment is provided for the skin.

Description

Technical Field

[0001] The invention belongs to the technical field of daily chemical production, and in particular relates to a facial cleanser with double cleansing effects and a preparation method thereof. Background Art

[0002] Facial cleanser is also called facial cleanser, which is a high-grade facial cleanser. It can clean facial dirt and remove makeup. A high-quality facial cleanser should have the functions of cleaning, nourishing, and protecting the skin. Generally speaking, ordinary facial cleansers do not have a whitening effect. If you add an appropriate amount of skin whitening agents (such as arbutin, V derivatives, placenta extracts, arbutin, kojic acid and its derivatives, etc.) to a high-quality facial cleanser, it will have a whitening effect after long-term use.

[0003] Generally speaking, facial cleansers are liquid products composed of oil phase, water phase, surfactants, moisturizers, nutrients and other ingredients. Creamy facial cleansers contain oil phase ingredients and are suitable for dry skin; crystal-like transparent products do not contain oil phase ingredients and can meet the needs of most consumers if the formula is properly adjusted. The surfactants in facial cleansers have five major functions: wetting, dispersing, foaming, cleaning and emulsifying, and are the main active ingredients in facial cleansers. In addition, according to the principle of like dissolves like, during the face washing process, the oil phase can dissolve the oil-soluble fat and excess oil on the face; the water phase can dissolve the water-soluble sweat stains on the face.

[0004] With the accelerated pace of modern life and the increasing severity of environmental pollution, people's demand for skin cleansing is no longer limited to surface cleaning, but more focused on the dual effects of deep cleansing and gentle skin care. Traditional cleansing products often fail to strike a balance between cleansing power and mildness. Either the cleansing power is too strong, causing the skin to become dry and tight, or the mildness is too high, resulting in incomplete cleansing, making it difficult to meet consumers' growing skin care needs.

[0005] Traditional facial cleansing products mostly rely on strong cleansers such as soap bases. Although they have strong cleaning power, long-term use will damage the skin barrier and cause dry and sensitive skin. Amino acid surfactants are gradually favored by the market for their mild and low-irritation properties, but they are often insufficient in cleaning power when used alone. While cleaning, the skin also needs to be fully moisturized and repaired. Summary of the invention

[0006] A facial cleanser with double cleansing effects and a preparation method thereof, characterized in that: a soap base is compounded with an amino acid surfactant to form a double cleansing system, so as to solve the problem that long-term use of strong soap-based cleansers will damage the skin barrier, causing dry and sensitive skin, while amino acid-based surfactants alone have insufficient cleaning power; a natural extract rose extract and aloe extract are fermented and modified using biological fermentation technology, so that they have a strong synergistic antibacterial effect and more antibacterial species;

[0007] At the same time, the pH regulator is specifically selected as citric acid, which can cooperate with potassium cocoyl glycinate to produce a self-crystallization effect, further enhancing the cleansing ability of the cleanser; at the same time, glycerin, hydrolyzed sodium hyaluronate and other ingredients are added to provide the cleanser with good hydration and moisturizing effects;

[0008] As a preferred embodiment, the specific cleaning formula is:

[0009] Component A soap base mixture: 6-8g lauric acid, 8-10g myristic acid, 2-5g stearic acid, 3-5g glyceryl stearate, 3-5mL glycerin, 10-15mL isopropyl myristate;

[0010] Component B amino acid surfactant: 8-12g tyrosine, 6-8mL potassium cocoyl glycinate, 4-6g sodium lauroyl glutamate, 7-12g glycine, 2-4g hydrolyzed sodium hyaluronate, 4-6g potassium hydroxide, 50-75mL deionized water;

[0011] Component C: 2-4 mL natural extract fermentation liquid, 1.3-1.8 g nano bamboo charcoal powder, 0.2-0.32 g flavor, 0.4-0.6 g preservative, 1-1.5 g citric acid;

[0012] The specific steps are:

[0013] S1. Add 6-8g of lauric acid, 8-10g of myristic acid, 2-5g of stearic acid, 3-5g of glyceryl stearate, 3-5mL of glycerol, and 10-15mL of isopropyl myristate in component A into a sterilized emulsifying pot, and stir and heat component A to 75-85°C until component A forms a uniform and stable emulsion, and then store the mixed component of phase A for standby use;

[0014] S2, put 8-12g tyrosine, 6-8mL potassium cocoyl glycinate, 4-6g sodium lauroyl glutamate, 7-12g glycine, 2-4g hydrolyzed sodium hyaluronate, 4-6g potassium hydroxide, and 50-75mL deionized water in component B into a sterilized water phase pot, and stir and heat the temperature in component B to 75-85°C until the materials therein are completely dissolved, and then store the mixed components of phase B for standby use;

[0015] S3. Preparation method of rose extract: After washing roses, add water to squeeze the juice, add 6 to 9 times the weight of the water used in the ethanol solution to the juice, and extract at 70 to 80°C for 20 to 30 minutes; then filter, centrifuge and concentrate to a concentration of 1.08 to 1.18 g / mL, and the obtained is the rose extract;

[0016] S4. Aloe extract preparation method: cut the cleaned aloe into small pieces and crush them, then extract the aloe for 30 to 50 minutes using a supercritical fluid extraction method to obtain the aloe extract; wherein the extraction solution is carbon dioxide, the extraction temperature is 55 to 60° C., and the extraction pressure is 16 to 18 MPa;

[0017] S5. Preparation of natural extract fermentation broth: ① Lactobacillus and Lactobacillus plantarum were inoculated on beef extract peptone solid culture medium, and cultured in an oven at 30-33°C until milky white colonies were formed and at 33-37°C until slightly yellowish colonies were formed; ② The activated Lactobacillus and Lactobacillus plantarum were transferred to a shake flask containing 500-1000 mL beef extract peptone liquid culture medium and fermented for 6-10 hours to obtain a fermentation broth with an inoculum size of 1.5×10 8 ~3.5×10 9 cfu·mL ～1 ③ Take a composition of rose extract and aloe extract in a mass ratio of 1: (1-2), with a total mass of 2.1-2.5 g, dissolve the composition in 20-30 mL of deionized water, ultrasonicate for 5-10 min until completely dissolved, take 10-20 mL of the obtained lactobacillus fermentation solution and inoculate it into the composition solution, and perform aerobic fermentation at 30-35 ° C for 12-24 h to obtain a first product; add 3% H2O2 solution to the first product to inactivate the lactobacillus, then add 10-20 mL of the plant lactobacillus fermentation solution, and ferment at 30-35 ° C for 12-24 h to obtain a first product; 35 ℃ for 12 to 24 hours of anaerobic fermentation to complete the fermentation; after the fermentation is completed, add 3% H2O2 solution to inactivate plant lactobacillus; ④ add CaCl2 solution to the fermentation liquid, let it stand for 15 to 30 minutes, centrifuge to obtain the supernatant, add 50% to 75% ethanol, centrifuge again, collect the supernatant, filter it, and then reflux extract it through a condensation reflux device for 30 to 60 minutes. After the extraction, keep the temperature at 55 ℃, adjust the pH to 9.0, add alkaline protease to stir and hydrolyze, concentrate after hydrolysis, and concentrate to 70 to 80% of the original volume to obtain a high-purity natural extract fermentation liquid;

[0018] S6. Prepare component C: dissolve 2-4 mL of natural extract fermentation liquid, 1.3-1.8 g of nano bamboo charcoal powder, 0.2-0.32 g of flavor, 0.4-0.6 g of preservative and 1-1.5 g of citric acid in deionized water, store and set aside;

[0019] S7, rapidly stirring the mixed components of phase A at a stirring rate of 1000-1300 r / min, and at the same time pumping the mixed components of phase B into the mixed components of phase A in the emulsifying pot, and keeping the mixed phase in the emulsifying pot warm and stirring at a temperature of 75-85° C. for 40-60 minutes to cause a saponification reaction;

[0020] S8. After the saponification reaction is completed, the mixture obtained in S3 is cooled to 40-50°C, and then the C component prepared by mixing according to its proportion is added thereto while stirring. After the addition is completed, it is continuously stirred at a rate of 300-500r / min until the mixture in the emulsification pot naturally cools down to 35-38°C, the stirring is stopped, and after sampling and testing are qualified, the material can be discharged.

[0021] Preferably: in step S1, 7 g of lauric acid, 9 g of myristic acid, 3.5 g of stearic acid, 4 g of glyceryl stearate, 4 mL of glycerol, and 12.5 mL of isopropyl myristate are put into a sterilized emulsifying pot, and the temperature in component A is stirred and heated to 80°C.

[0022] Preferably: in step S2, 10 g of tyrosine, 7 mL of potassium cocoyl glycinate, 5 g of sodium lauroyl glutamate, 9 g of glycine, 3 g of hydrolyzed sodium hyaluronate, 5 g of potassium hydroxide, and 60 mL of deionized water are put into a sterilized water phase pot, and the temperature in component B is stirred and heated to 80°C.

[0023] Preferably: the preparation method of the rose extract in step S3 is as follows: after the washed roses are added with water to squeeze out the juice, an ethanol solution of 8 times the weight of the water used is added to the juice, and the juice is extracted at 75°C for 25 minutes; and then filtered, centrifuged and concentrated to a concentration of 1.10 g / mL.

[0024] Preferably: the method for preparing the aloe extract in step S4 is as follows: the cleaned aloe is cut into small pieces and then crushed, and then the aloe is extracted by supercritical fluid extraction for 40 minutes to obtain the aloe extract; wherein the extraction solution in the extraction process is carbon dioxide, the extraction temperature is 58°C, and the extraction pressure is 17MPa.

[0025] Preferably: the preparation method of the solid culture medium in step S5 is: beef extract: 3.0g, peptone: 10.0g, NaCl: 5.0g, agar: 20g, water: 1000mL, and the pH is adjusted to 7.0 at the same time; the preparation method of the liquid culture medium is: if 1000mL of liquid culture medium is configured, 3g of beef extract, 10g of peptone, 5g of sodium chloride are added to distilled water, and the pH value is adjusted to 7.2 with 5mol / L sodium hydroxide solution. Lactobacillus was purchased from Beijing Biological Collection Center, and the plant lactobacillus used was purchased from Guangdong Microbiological Culture Collection Center.

[0026] Preferably: in step S6, component C is prepared, 3 mL of natural extract fermentation liquid, 1.55 g of nano bamboo charcoal powder, 0.26 g of flavor, 0.5 g of preservative and 1.25 g of citric acid are dissolved in 5 mL of deionized water, and stored for later use.

[0027] Preferably, in step S7, the phase A mixed component is rapidly stirred at a stirring rate of 1200 r / min, and the phase B mixed component is pumped into the phase A mixed component in the emulsifying pot, and the mixed phase in the emulsifying pot is kept warm and stirred at a temperature of 80° C. for 50 minutes to cause a saponification reaction.

[0028] Preferably: in step S8, after the saponification reaction is completed, the mixture obtained in S3 is cooled to 45°C, and then the C component prepared by mixing according to its proportion is added thereto while stirring. After the addition is completed, it is continuously stirred at a rate of 400 r / min until the mixture in the emulsification pot is naturally cooled to 36°C.

[0029] Due to the adoption of the above technical solution, the technical progress achieved by the present invention is:

[0030] 1. The method of combining soap base and amino acid active ingredients used in the present invention solves the problem that long-term use of soap-based facial cleansers will damage the skin barrier and make the facial skin dry and sensitive. At the same time, the soap base and amino acid active ingredients interact with each other to promote double cleansing and enhance the cleaning ability of the facial cleanser.

[0031] 2. The rose extract used in the present invention can effectively act on the pores. While improving the cleaning ability of the facial cleanser, the flavonoids and other chemical components in the rose have a preventive and therapeutic effect on cardiovascular and cerebrovascular diseases, and the MIC of the 4'-hydroxy-7-(3-hydroxypropionyl)-6-methoxy-isoflavone structure to Staphylococcus aureus (MRSA) strain is 90 46±4mg / L, close to the MIC of levofloxacin 90 (53±5mg / L), has antibacterial activity; at the same time, the scent of roses can also enhance the user experience.

[0032] 3. The present invention uses citric acid as a pH regulator. Citric acid has the function of regulating the pH value of the facial cleanser, which helps to maintain the natural acid-base balance of the skin and reduce irritation to the skin. An appropriate amount of citric acid can help remove aged keratin on the skin surface, promote skin metabolism, and make the skin smoother and more delicate. Citric acid also has the effect of whitening and brightening the skin tone, can reduce dull skin, and make the skin look brighter. Citric acid and potassium cocoyl glycinate interact with each other, and self-crystallization can improve the cleaning ability. In addition, citric acid can soften the stratum corneum, and potassium cocoyl glycinate can effectively remove dirt. The combination of the two makes the cleaning effect more significant. At the same time, citric acid helps to maintain the acid-base balance of the skin, while potassium cocoyl glycinate can maintain skin moisture. The two work together to make the skin feel more comfortable after washing with the facial cleanser.

[0033] 4. The present invention uses lactobacillus, which can regulate the microecological balance of the skin, inhibit the reproduction of harmful bacteria, and provide a good microbial environment for the skin; the lactobacillus fermentation product is rich in amino acids and microorganisms, which can reduce water loss.

[0034] 5. The present invention uses aloe extract, which is rich in moisturizing factors, can replenish moisture to the skin and improve the symptoms of dry skin. At the same time, it can also form a water-locking film on the skin surface, which helps to reduce the loss of skin moisture and make the skin tender and shiny. Aloe extract contains anthraquinone compounds, such as aloesin and aloe-emodin glycosides, which have bactericidal, antibacterial and anti-inflammatory effects, can improve skin inflammatory lesions and promote wound healing; in addition, aloe extract can also prevent and improve skin problems, such as acne, blackheads, etc.; certain ingredients in aloe can dilute the dullness of the skin and make the skin look brighter; the mucus in aloe contains a unique protein, which helps prevent cell aging and also has a certain effect on the treatment of chronic allergies.

[0035] 6. The present invention uses biological fermentation technology to ferment and modify the natural extracts of rose extract and aloe extract. The obtained natural extract fermentation liquid (rose extract, aloe extract, Lactobacillus CICC 6075 and Lactobacillus plantarum ATCC 14917) has a synergistic effect with the amino acid surfactant, which enhances the effect of cleaning and protecting the skin. At the same time, under the fermentation conditions, the fermentation product of the two extracts (natural extract fermentation liquid) can enhance the cleaning effect of the facial cleanser and has a strong synergistic antibacterial effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0037] Figure 1 It is a comparison of the oil cleaning rate and dirt cleaning rate of Example 1 and Comparative Example 1 and Comparative Example 2 of the present invention;

[0038] Figure 2 It is a comparison of tyrosinase inhibition rate and DPPH free radical scavenging rate of Example 2, Comparative Example 3, Comparative Example 4 and Comparative Example 5.

[0039] Figure 3 It is a comparison of the skin moisture content of Example 1, Comparative Example 1 and Comparative Example 2 three hours after use.

[0040] Figure 4 Comparison of skin R2 of Example 1, Comparative Example 1 and Comparative Example 2 three hours after use. DETAILED DESCRIPTION

[0041] In order to make the technical problems, technical solutions and beneficial effects to be solved by the present invention more clearly understood, the present invention is further described in detail in combination with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the content of the present invention and are not used to limit the present invention.

[0042] Example 1

[0043] This example illustrates that the synergistic use of soap base and amino acid active ingredients not only enhances the cleansing ability of the facial cleanser, but also provides good protection and moisturizing effects on the skin;

[0044] A facial cleanser with double cleansing effects, the specific production steps are:

[0045] Component A soap base mixture: 6g lauric acid, 8g myristic acid, 2g stearic acid, 3g glyceryl stearate, 3mL glycerin, 10mL isopropyl myristate;

[0046] Component B amino acid surfactant: 8g tyrosine, 6mL potassium cocoyl glycinate, 4g sodium lauroyl glutamate, 7g glycine, 2g hydrolyzed sodium hyaluronate, 4g potassium hydroxide, 50mL deionized water;

[0047] Component C: 2 mL natural extract fermentation liquid, 1.3 g nano bamboo charcoal powder, 0.2 g flavor, 0.4 g preservative, 1 g citric acid;

[0048] The specific steps are:

[0049] S1. Add 6g of lauric acid, 8g of myristic acid, 2g of stearic acid, 3g of glyceryl stearate, 3mL of glycerol, and 10mL of isopropyl myristate in component A into a sterilized emulsifying pot, and stir and heat component A to 75°C until component A forms a uniform and stable emulsion, and then store the mixed component A for standby use;

[0050] S2, put 8g tyrosine, 6mL potassium cocoyl glycinate, 4g sodium lauroyl glutamate, 7g glycine, 2g hydrolyzed sodium hyaluronate, 4g potassium hydroxide, and 50mL deionized water in component B into a sterilized water phase pot, and stir and heat the temperature in component B to 75°C until the materials therein are completely dissolved, and then store the mixed components of phase B for standby use;

[0051] S3. Preparation method of rose extract: After washing roses, add water to squeeze juice, add ethanol solution 6 times the weight of water to the juice, and extract at 70°C for 20 minutes; then filter, centrifuge and concentrate to a concentration of 1.08g / mL, and the obtained product is rose extract;

[0052] S4. Aloe extract preparation method: cut the cleaned aloe into small pieces and crush them, then extract the aloe for 30 minutes using supercritical fluid extraction to obtain the aloe extract; wherein the extraction solution is carbon dioxide, the extraction temperature is 55° C., and the extraction pressure is 16 MPa;

[0053] S5. Preparation of natural extract fermentation broth: ① Lactobacillus and Lactobacillus plantarum were inoculated on beef extract peptone solid culture medium, and cultured in an oven at 30°C until milky white colonies were formed and at 33°C until slightly yellowish colonies were formed; ② The activated Lactobacillus and Lactobacillus plantarum were transferred to a shake flask containing 500 mL beef extract peptone liquid culture medium and fermented for 6 h to obtain a fermentation broth with an inoculum size of 1.5×10 8 ~3.5×10 9 cfu·mL ～1③ Take a composition of rose extract and aloe extract in a mass ratio of 1: (1-2), the total mass of the composition is 2.1g, dissolve the composition in 20mL deionized water, ultrasonicate for 5min until completely dissolved, take 10mL of the obtained lactobacillus fermentation solution and inoculate it into the composition solution, and perform aerobic fermentation at 30°C for 12h to obtain the first product; add 3% H2O2 solution to the first product to inactivate the lactobacillus, then add 10mL of the plant lactobacillus fermentation solution, and perform aerobic fermentation at 30°C for 12h to obtain the first product; 12h of anaerobic fermentation to complete the fermentation; after the fermentation is completed, 3% H2O2 solution is added to inactivate Lactobacillus plantarum; ④ CaCl2 solution is added to the fermentation liquid, and the supernatant is centrifuged after standing for 15min, 50% ethanol is added, and centrifugation is performed again, the supernatant is collected, and suction filtration is performed, and then reflux extraction is performed through a condensation reflux device for 30min. After the extraction, the temperature is maintained at 55°C, the pH is adjusted to 9.0, and alkaline protease is added for stirring and hydrolysis. After the hydrolysis, it is concentrated, and a high-purity natural extract fermentation liquid can be obtained after being concentrated to 70% of the original volume;

[0054] S6. Prepare component C: dissolve 2 mL of natural extract fermentation liquid, 1.3 g of nano bamboo charcoal powder, 0.2 g of flavor, 0.4 g of preservative and 1 g of citric acid in deionized water, and store for later use;

[0055] S7, rapidly stirring the mixed components of phase A at a stirring rate of 1000 r / min, and at the same time pumping the mixed components of phase B into the mixed components of phase A in the emulsifying pot, and keeping the mixed phase in the emulsifying pot warm and stirring at a temperature of 75° C. for 40 minutes to cause a saponification reaction;

[0056] S8. After the saponification reaction is completed, the mixture obtained in S3 is cooled to 40°C, and then the C component prepared by mixing according to its proportion is added thereto while stirring. After the addition is completed, it is continuously stirred at a rate of 300r / min until the mixture in the emulsification pot naturally cools down to 35°C, the stirring is stopped, and after sampling and testing are qualified, the material can be discharged.

[0057] Comparative Example 1: Step S7 is omitted, and phase B and phase C are directly mixed, and the other steps are the same as those of Example 1;

[0058] Comparative Example 2: Step S7 is omitted, and phase A and phase C are directly mixed. The other steps are the same as those in Example 1.

[0059] (1) Cleaning ability

[0060] Artificial dirt test method

[0061] S1: Use standard dirt to apply on the simulated skin material, and then use the facial cleansing milk prepared in Example 1 and Comparative Examples 1-2 to clean. The standard dirt is composed of 65% calcium carbonate, 5% urea, 5% magnesium carbonate, 0.8% calcium bicarbonate, 0.5% egg white liquid (or other animal ingredients), and 23.7% other organic matter (glucose, sodium chloride, animal fat, plant fiber, etc.);

[0062] S2: Use the HD-HC500 surface residual oil detector produced by Shandong Holder Electronic Technology Co., Ltd. to detect the oil content of the simulated skin before and after cleaning, and calculate the oil cleaning rate {oil removal rate = (skin oil content before use - skin oil content after use) / skin oil content before use × 100%};

[0063] S3: Use the online surface residual oil measuring instrument produced by SITA of Germany to detect the dirt content of the simulated skin before and after cleaning, and calculate the dirt cleaning rate {oil removal rate = (skin dirt content before use - skin dirt content after use) / skin dirt content before use × 100%}; the obtained data are recorded in Table 1;

[0064] Protective and moisturizing properties

[0065] Skin test method

[0066] S1: 120 female subjects aged 20 to 30 with dry skin were selected and randomly divided into 3 groups, 40 people in each group, and marked as Example 1 and Comparative Examples 1 to 2 respectively;

[0067] S2: Before the test, 140 subjects were placed in an environment with a room temperature of (23±1)℃ and a humidity of (50±5)% for 30 minutes, and the skin moisture content was detected using a corneometer CM825 test probe.

[0068] S3: The three groups of subjects were respectively treated with the facial cleansers prepared in Example 1 and Comparative Examples 1-2 that matched the groups. After 1 hour, 2 hours, and 3 hours of use, the water content of the stratum corneum on the faces of the subjects was measured once using a corneometer CM825 test probe. The elasticity index R2 of a fixed area was detected using a skin elasticity tester MPA580 (CK, Germany). The average values ​​of the obtained test data are recorded in Table 1:

[0069] Table 1 Comparison of water-locking and moisturizing effects within 3 hours, and comparison of oil and dirt cleaning rates

[0070]

[0071] The test results show that mixing soap-based cleansers and amino acid surfactants not only makes the cleanser more powerful, but also has good moisturizing and water-locking effects on the skin.

[0072] Example 2

[0073] This example proves that the facial cleanser uses citric acid and has the effect of whitening and brightening the skin tone, which can reduce dull skin and make the skin look brighter.

[0074] A facial cleanser with double cleansing effects, the specific production steps are:

[0075] Component A soap base mixture: 7g lauric acid, 9g myristic acid, 4g stearic acid, 4g glyceryl stearate, 4mL glycerol, 13mL isopropyl myristate;

[0076] Component B amino acid surfactant: 10g tyrosine, 7mL potassium cocoyl glycinate, 5g sodium lauroyl glutamate, 9g glycine, 3g hydrolyzed sodium hyaluronate, 5g potassium hydroxide, 60mL deionized water;

[0077] Component C: 3 mL natural extract fermentation liquid, 1.6 g nano bamboo charcoal powder, 0.26 g flavor, 0.5 g preservative, 1.3 g citric acid;

[0078] The specific steps are:

[0079] S1. Add 7g of lauric acid, 9g of myristic acid, 4g of stearic acid, 4g of glyceryl stearate, 4mL of glycerol, and 13mL of isopropyl myristate in component A into a sterilized emulsifying pot, and stir and heat component A to 80°C until component A forms a uniform and stable emulsion, and then store the mixed component A for standby use;

[0080] S2, put 10g tyrosine, 7mL potassium cocoyl glycinate, 5g sodium lauroyl glutamate, 9g glycine, 3g hydrolyzed sodium hyaluronate, 5g potassium hydroxide, and 60mL deionized water in component B into a sterilized water phase pot, and stir and heat the temperature in component B to 80°C until the materials therein are completely dissolved, and then store the mixed components of phase B for standby use;

[0081] S3. Preparation method of rose extract: After washing roses, add water to squeeze juice, add ethanol solution of 8 times the weight of water to the juice, and extract at 75°C for 25 minutes; then filter, centrifuge and concentrate to a concentration of 1.13g / mL in sequence, and the obtained product is rose extract;

[0082] S4. Aloe extract preparation method: cut the cleaned aloe into small pieces and crush them, then extract the aloe for 40 minutes using supercritical fluid extraction to obtain the aloe extract; wherein the extraction solution is carbon dioxide, the extraction temperature is 57° C., and the extraction pressure is 17 MPa;

[0083] S5. Preparation of natural extract fermentation broth: ① Lactobacillus and Lactobacillus plantarum were inoculated on beef extract peptone solid culture medium, and cultured in an oven at 32°C until milky white colonies were formed and at 35°C until slightly yellowish colonies were formed; ② The activated Lactobacillus and Lactobacillus plantarum were transferred to a shake flask containing 800 mL beef extract peptone liquid culture medium and fermented for 8 h to obtain a fermentation broth with an inoculum size of 1.5×10 8 ~3.5×10 9 cfu·mL ～1 ③ Take a composition of rose extract and aloe extract in a mass ratio of 1:1.5, with a total mass of 2.3g, dissolve the composition in 25mL deionized water, ultrasonicate for 8min until completely dissolved, take 15mL of the obtained lactobacillus fermentation solution and inoculate it into the composition solution, and perform aerobic fermentation at 33°C for 18h to obtain the first product; add 3% H2O2 solution to the first product to inactivate the lactobacillus, then add 15mL of plant lactobacillus fermentation solution, and perform aerobic fermentation at 33°C for 18h to obtain the first product; 8h of anaerobic fermentation to complete the fermentation; after the fermentation is completed, 3% H2O2 solution is added to inactivate Lactobacillus plantarum; ④ CaCl2 solution is added to the fermentation liquid, and the supernatant is centrifuged after standing for 25 minutes, 60% ethanol is added, and centrifugation is performed again, the supernatant is collected, and suction filtration is performed, and then reflux extraction is performed through a condensation reflux device for 45 minutes. After the extraction, the temperature is maintained at 55°C, the pH is adjusted to 9.0, and alkaline protease is added for stirring and hydrolysis. After the hydrolysis, it is concentrated, and when it is concentrated to 75% of the original volume, a high-purity natural extract fermentation liquid can be obtained;

[0084] S6. Prepare component C: dissolve 3 mL of natural extract fermentation liquid, 1.6 g of nano bamboo charcoal powder, 0.26 g of flavor, 0.5 g of preservative and 1.3 g of citric acid in deionized water, store and set aside;

[0085] S7, rapidly stirring the mixed components of phase A at a stirring rate of 1150 r / min, and at the same time pumping the mixed components of phase B into the mixed components of phase A in the emulsifying pot, and stirring the mixed phase in the emulsifying pot at a temperature of 80° C. for 50 minutes to cause a saponification reaction;

[0086] S8. After the saponification reaction is completed, the mixture obtained in S3 is cooled to 45°C, and then the C component prepared by mixing according to its proportion is added thereto while stirring. After the addition is completed, it is continuously stirred at a rate of 400r / min until the mixture in the emulsification pot naturally cools down to 36°C, the stirring is stopped, and after sampling and testing are qualified, the material can be discharged.

[0087] Comparative Example 3: In step S6, citric acid is replaced by acetic acid, and the other steps are the same as those in Example 2;

[0088] Comparative Example 4: In step S6, citric acid is replaced by malic acid, and the other steps are the same as those in Example 2;

[0089] Comparative Example 5: In step S6, citric acid is replaced by lactic acid, and the other steps are the same as those in Example 2;

[0090] (1) Whitening effect

[0091] This test uses the tyrosinase inhibition method to detect:

[0092] Experimental reagents and instruments: tyrosinase solution (prepared from fresh potatoes), L-tyrosine, arbutin, disodium hydrogen phosphate, sodium dihydrogen phosphate, thermostat, ultraviolet spectrophotometer, centrifuge.

[0093] Reagent preparation

[0094] Sodium phosphate buffer (pH=6.8): Weigh 1 g of sodium dihydrogen phosphate and 1.186 g of disodium hydrogen phosphate, add a small amount of deionized water to dissolve, make up to 500 mL, and store at 4°C for later use.

[0095] Test solution: Weigh 0.1 g of the facial cleansing milk obtained in Example 2 and Comparative Examples 3 to 5 as a sample, and dissolve them in 20 mL of deionized water to obtain a 5 mg / mL test solution.

[0096] Tyrosinase solution: Wash the potatoes and pre-cool them at 4℃ for about 4 hours. Peel them and cut them into pieces about 1.0 cm ～3 Dice, freeze at -20℃ overnight, weigh, add 4℃ precooled sodium phosphate buffer at a ratio of 1:1 (W:V), make a homogenate with a tissue grinder, filter through 3 layers of gauze, centrifuge the filtrate at 4000r / min for 10min, and the supernatant is the obtained tyrosinase solution, which should be stored at 4℃.

[0097] Negative control: Weigh 0.1 g of arbutin and dissolve it in 20 mL of deionized water to obtain a 5 mg / mL negative control stock solution.

[0098] Detection Methods

[0099] During the experiment, take 8 test tubes, add 2.9mL sodium phosphate buffer (pH=6.8), 1.0mL of different test solutions, 1.0mL of negative control mother solution, and 0.1mL of tyrosinase solution into the test tubes respectively, and place in a 30℃ water bath for 10min. Then add 2.0mL of L-tyrosine solution with a concentration of 7.5mmol / L, and start timing immediately. Measure the absorbance at a wavelength of 475nm after 20min of reaction. After the measurement, use the following formula to calculate the inhibition rate of the test solution on tyrosinase, inhibition rate = [(AB) / A]×100%, where A is the absorbance value of the negative control group, and B is the absorbance value of the test solution. The experimental results are shown in Table 2;

[0100] Table 2 Comparison of tyrosinase inhibition rate and DPPH free radical scavenging rate

[0101] project Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Tyrosinase inhibition rate (%) 80.92±2.19 64.25±2.58 52.32±1.58 65.96±2.63 DPPH free radical scavenging rate (%) 78.56±5.25 51.73±5.19 36.80±5.64 50.58±4.75

[0102] The above results show that using citric acid as a regulator has a significant effect on skin whitening.

[0103] Example 3

[0104] The two examples prove that the facial cleanser uses the fermented product of two plant extracts (natural extract fermentation liquid) to enhance the cleansing effect of the facial cleanser, and has a strong synergistic antibacterial effect, and the number of antibacterial species is increased.

[0105] A facial cleanser with double cleansing effects, the specific production steps are:

[0106] Component A soap base mixture: 6-8g lauric acid, 8-10g myristic acid, 2-5g stearic acid, 3-5g glyceryl stearate, 3-5mL glycerin, 10-15mL isopropyl myristate;

[0107] Component B amino acid surfactant: 8-12g tyrosine, 6-8mL potassium cocoyl glycinate, 4-6g sodium lauroyl glutamate, 7-12g glycine, 2-4g hydrolyzed sodium hyaluronate, 4-6g potassium hydroxide, 50-75mL deionized water;

[0108] Component C: 2-4 mL natural extract fermentation liquid, 1.3-1.8 g nano bamboo charcoal powder, 0.2-0.32 g flavor, 0.4-0.6 g preservative, 1-1.5 g citric acid;

[0109] The specific steps are:

[0110] S1. Add 8g of lauric acid, 10g of myristic acid, 5g of stearic acid, 5g of glyceryl stearate, 5mL of glycerol, and 15mL of isopropyl myristate in component A into a sterilized emulsifying pot, and heat component A to 85°C with stirring until component A forms a uniform and stable emulsion, and then store the mixed component A for standby use;

[0111] S2, put 12g tyrosine, 8mL potassium cocoyl glycinate, 6g sodium lauroyl glutamate, 12g glycine, 4g hydrolyzed sodium hyaluronate, 6g potassium hydroxide, and 75mL deionized water in component B into a sterilized water phase pot, and stir and heat the temperature in component B to 85°C until the materials therein are completely dissolved, and then store the mixed components of phase B for standby use;

[0112] S3. Preparation method of rose extract: After washing roses, add water to squeeze juice, add ethanol solution of 9 times the weight of water to the juice, and extract at 80°C for 30 minutes; then filter, centrifuge and concentrate to a concentration of 1.18g / mL in sequence, and the obtained product is rose extract;

[0113] S4. Aloe extract preparation method: cut the cleaned aloe into small pieces and crush them, then extract the aloe for 50 minutes using supercritical fluid extraction to obtain the aloe extract; wherein the extraction solution is carbon dioxide, the extraction temperature is 60° C., and the extraction pressure is 18 MPa;

[0114] S5. Preparation of natural extract fermentation broth: ① Lactobacillus and Lactobacillus plantarum were inoculated on beef extract peptone solid culture medium, and cultured in an oven at 33°C until milky white colonies were formed and at 37°C until slightly yellowish colonies were formed; ② The activated Lactobacillus and Lactobacillus plantarum were transferred to a shake flask containing 1000 mL beef extract peptone liquid culture medium and fermented for 10 h to obtain a fermentation broth with an inoculum size of 1.5×10 8 ~3.5×10 9 cfu·mL ～1③ Take a composition of rose extract and aloe extract in a mass ratio of 1:2, with a total mass of 2.5g, dissolve the composition in 30mL deionized water, ultrasonicate for 10min until completely dissolved, take 20mL of the obtained lactobacillus fermentation solution and inoculate it into the composition solution, and perform aerobic fermentation at 35°C for 24h to obtain the first product; add 3% H2O2 solution to the first product to inactivate the lactobacillus, then add 20mL of the plant lactobacillus fermentation solution, and perform aerobic fermentation at 35°C for 24h to obtain the first product; 4h of anaerobic fermentation to complete the fermentation; after the fermentation is completed, 3% H2O2 solution is added to inactivate Lactobacillus plantarum; ④ CaCl2 solution is added to the fermentation liquid, and the supernatant is centrifuged after standing for 30min, 75% ethanol is added, and centrifugation is performed again, the supernatant is collected, and suction filtration is performed, and then reflux extraction is performed for 60min through a condensation reflux device. After the extraction, the temperature is maintained at 55°C, the pH is adjusted to 9.0, and alkaline protease is added for stirring and hydrolysis. After the hydrolysis, it is concentrated, and when it is concentrated to 80% of the original volume, a high-purity natural extract fermentation liquid can be obtained;

[0115] S6. Prepare component C: dissolve 4 mL of natural extract fermentation liquid, 1.8 g of nano bamboo charcoal powder, 0.32 g of flavor, 0.6 g of preservative and 1.5 g of citric acid in deionized water, and store for later use;

[0116] S7, rapidly stirring the mixed components of phase A at a stirring rate of 1300 r / min, and at the same time pumping the mixed components of phase B into the mixed components of phase A in the emulsifying pot, and keeping the mixed phase in the emulsifying pot warm and stirring at a temperature of 85° C. for 60 minutes to cause a saponification reaction;

[0117] S8. After the saponification reaction is completed, the mixture obtained in S3 is cooled to 50°C, and then the C component prepared by mixing according to its proportion is added thereto while stirring. After the addition is completed, it is continuously stirred at a rate of 500r / min until the mixture in the emulsification pot naturally cools down to 38°C, the stirring is stopped, and after sampling and testing are qualified, the material can be discharged.

[0118] Comparative Example 6: In step S5, no rose extract was added, and the other steps were the same as those in Example 3;

[0119] Comparative Example 7: In step S5, no aloe extract was added, and the other steps were the same as those in Example 3;

[0120] Comparative Example 8: In step S5, Lactobacillus plantarum was used alone for fermentation, and the other steps were the same as those in Example 3;

[0121] Comparative Example 9: In step S5, lactobacillus was used alone for fermentation, and the other steps were the same as those in Example 3.

[0122] (1) Cleaning effect test

[0123] Artificial dirt test method

[0124] Standard dirt was applied to the simulated skin material, and then cleaned with the facial cleansers prepared in the use examples and comparative examples 4 to 7. The standard dirt consisted of 65% calcium carbonate, 5% urea, 5% magnesium carbonate, 0.8% calcium bicarbonate, 0.5% egg white solution (or other animal ingredients), and 23.7% other organic matter (glucose, sodium chloride, animal fat, plant fiber, etc.);

[0125] The HD-HC500 surface residual oil detector produced by Shandong Holder Electronic Technology Co., Ltd. was used to detect the oil content of the simulated skin before and after cleaning, and the oil cleaning rate was calculated {oil removal rate = (skin oil content before use - skin oil content after use) / skin oil content before use × 100%};

[0126] The dirt content of the simulated skin before and after cleaning was detected using an online surface residual oil measuring instrument produced by SITA of Germany, and the dirt cleaning rate {oil removal rate = (skin dirt content before use - skin dirt content after use) / skin dirt content before use × 100%} was calculated; the obtained data are recorded in Table 3;

[0127] Table 3 Grease cleaning rate and dirt cleaning rate of Example 3 and Comparative Examples 6 to 9

[0128] Test items Grease cleaning rate / % Dirt cleaning rate / % Example 3 65.23±0.07％ 81.56±0.05％ Comparative Example 6 58.25±0.05％ 74.53±0.06％ Comparative Example 7 61.23±0.06％ 76.23±0.07％ Comparative Example 8 60.54±0.06％ 75.56±0.06％ Comparative Example 9 59.25±0.07％ 74.28±0.07％

[0129] (2) Antibacterial effect

[0130] Inhibition zone method ~ punching method

[0131] The plate culture medium is used: peptone 10g / L, yeast powder 5g / L, NaCl110 g / L, agar powder 20g / L, pH 7.8; before the test, the test sample should be rinsed with sterile water and dried naturally, and the control sample should be sterilized by high pressure wet heat and then dried. Other instruments used in the test can be sterilized by high temperature wet heat or dry heat method; the hole diameter of the culture medium is 6mm; Propionibacterium acnes (ATCC 11827) is spread on the culture medium with a coating rod; the facial cleansing milk of each embodiment 3 and comparative examples 6 to 9 is diluted 10 times and kept at 35°C to improve the fluidity of the facial cleansing milk, and then the facial cleansing milk is injected into the hole of the culture medium, filled and not overflowing outside the hole, and a group of physiological saline equal to the amount of facial cleansing milk is set as a blank control group; under the conditions of (37±1)°C and relative humidity greater than 90%, it is placed upright for 16h to 18h, and the diameter of the antibacterial zone is measured and recorded with a vernier caliper. There should be no antibacterial zone in the blank control group. The experiment was repeated three times and the average inhibition zone data are recorded in Table 4;

[0132] Table 4 Inhibition zone diameter

[0133] Diameter of inhibition zone (mm) Example 3 31.21±1.2 Comparative Example 6 15.65±1.4 Comparative Example 7 16.58±0.8 Comparative Example 8 14.56±1.5 Comparative Example 9 15.15±1.2

[0134] The experimental results show that the rose extract and aloe extract modified by lactobacillus and plantarum fermentation have a synergistic effect, which can make the cleanser have a better cleaning effect and have a strong synergistic antibacterial effect.

[0135] The embodiments described above are merely descriptions of the preparation process of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solution of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A facial cleanser with double cleansing effects and a preparation method thereof, Features: The specific preparation steps are: S1. Add 6-8g of lauric acid, 8-10g of myristic acid, 2-5g of stearic acid, 3-5g of glyceryl stearate, 3-5mL of glycerol, and 10-15mL of isopropyl myristate in component A into a sterilized emulsifying pot, and stir and heat component A to 75-85°C until component A forms a uniform and stable emulsion, and then store the mixed component of phase A for standby use; S2, put 8-12g tyrosine, 6-8mL potassium cocoyl glycinate, 4-6g sodium lauroyl glutamate, 7-12g glycine, 2-4g hydrolyzed sodium hyaluronate, 4-6g potassium hydroxide, and 50-75mL deionized water in component B into a sterilized water phase pot, and stir and heat component B to 75-85°C until the materials therein are completely dissolved, and then store the mixed components of phase B for standby use; S3. Preparation method of rose extract: After washing roses, add water to squeeze the juice, add 6 to 9 times the weight of the water used in the ethanol solution to the juice, and extract at 70 to 80°C for 20 to 30 minutes; then filter, centrifuge and concentrate to a concentration of 1.08 to 1.18 g / mL, and the obtained is the rose extract; S4. Aloe extract preparation method: cut the cleaned aloe into small pieces and crush them, then extract the aloe for 30 to 50 minutes using a supercritical fluid extraction method to obtain the aloe extract; wherein the extraction agent solution is carbon dioxide, the extraction temperature is 55 to 60° C., and the extraction pressure is 16 to 18 MPa; S5. Preparing natural extract fermentation broth: Lactobacillus CICC 6075 and Lactobacillus plantarum ATCC 14917 were inoculated on beef extract peptone solid culture medium, and cultured in an oven at 30-33° C. and 33-37° C., respectively, until milky white colonies and slightly yellowish colonies were formed; the activated Lactobacillus and Lactobacillus plantarum were transferred to a shake flask containing 500-1000 mL beef extract peptone liquid culture medium and fermented for 6-10 h to obtain a fermentation broth with an inoculum size of 1.5×10 8 ~3.5×10 9 cfu·mL ～1 Lactobacillus and plantarum fermented bacterial liquid; taking a composition of rose extract and aloe extract in a mass ratio of 1: (1-2), the total mass of the composition is 2.1-2.5g, dissolving the composition in 20-30mL deionized water, ultrasonicating for 5-10min until completely dissolved, taking 10-20mL of the obtained lactobacillus fermented bacterial liquid and inoculating it into the composition solution, and aerobic fermentation is carried out at 30-35°C for 12-24h to obtain a first product; adding 3% H 2 O 2 The solution was used to inactivate lactobacillus, and then 10-20 mL of plant lactobacillus fermentation liquid was added, and the fermentation was completed by anaerobic fermentation at 30-35°C for 12-24 hours. After the fermentation was completed, 3% H 2 O 2 solution to inactivate Lactobacillus plantarum; CaCl was added to the fermentation broth. 2 The solution is allowed to stand for 15 to 30 minutes, and then centrifuged to obtain the supernatant, 50% to 75% ethanol is added, and the solution is centrifuged again to collect the supernatant, and then filtered, and then refluxed through a condensation reflux device for 30 to 60 minutes for extraction. After the extraction, the temperature is maintained at 55° C., and after the pH is adjusted to 9.0, alkaline protease is added for stirring and hydrolysis, and after the hydrolysis, the solution is concentrated. When the solution is concentrated to 70 to 80% of the original volume, a high-purity natural extract fermentation liquid can be obtained; S6. Prepare component C: dissolve 2-4 mL of natural extract fermentation liquid, 1.3-1.8 g of nano bamboo charcoal powder, 0.2-0.32 g of flavor, 0.4-0.6 g of preservative and 1-1.5 g of citric acid in deionized water, store and set aside; S7, rapidly stirring the mixed components of phase A at a stirring rate of 1000-1300 r / min, and at the same time pumping the mixed components of phase B into the mixed components of phase A in the emulsifying pot, and keeping the mixed phase in the emulsifying pot warm and stirring at a temperature of 75-85° C. for 40-60 minutes to cause a saponification reaction; S8. After the saponification reaction is completed, the mixture obtained in S3 is cooled to 40-50°C, and then the C component prepared by mixing according to its proportion is added thereto while stirring. After the addition is completed, it is continuously stirred at a rate of 300-500r / min until the mixture in the emulsification pot naturally cools down to 35-38°C, the stirring is stopped, and after sampling and testing are qualified, the material can be discharged.

2. A method for preparing a facial cleanser with double cleansing effects according to claim 1, Features: In step S1, 7 g of lauric acid, 9 g of myristic acid, 3.5 g of stearic acid, 4 g of glyceryl stearate, 4 mL of glycerol, and 12.5 mL of isopropyl myristate are placed in a sterilized emulsifying pot, and the temperature in component A is stirred and heated to 80°C.

3. A method for preparing a facial cleanser with double cleansing effects according to claim 1, Features: In step S2, 10 g of tyrosine, 7 mL of potassium cocoyl glycinate, 5 g of sodium lauroyl glutamate, 9 g of glycine, 3 g of hydrolyzed sodium hyaluronate, 5 g of potassium hydroxide, and 60 mL of deionized water are added into a sterilized water phase pot, and component B is stirred and heated to 80°C.

4. A method for preparing a facial cleanser with double cleansing effects according to claim 1, Features: The preparation method of the rose extract in step S3 is as follows: after washing the roses, add water to squeeze the juice, add an ethanol solution of 8 times the weight of the water used to the juice, and extract at 75°C for 25 minutes; then filter, centrifuge and concentrate in sequence to a concentration of 1.10 g / mL.

5. A method for preparing a facial cleanser with double cleansing effects according to claim 1, Features: The method for preparing the aloe extract in step S4 is as follows: the cleaned aloe is cut into small pieces and then crushed, and then the aloe is extracted by supercritical fluid extraction for 40 minutes to obtain the aloe extract; wherein the extraction solvent in the extraction process is carbon dioxide, the extraction temperature is 58° C., and the extraction pressure is 17 MPa.

6. A method for preparing a facial cleanser with double cleansing effects according to claim 1, Features: The preparation method of the solid culture medium in step S5 is: beef extract: 3.0g, peptone: 10.0g, NaCl: 5.0g, agar: 20g, water: 1000mL, and the pH is adjusted to 7.0 at the same time; the preparation method of the liquid culture medium is: if 1000mL of liquid culture medium is prepared, 3g of beef extract, 10g of peptone, 5g of sodium chloride are added to distilled water, and the pH value is adjusted to 7.2 with 5mol / L sodium hydroxide solution. Lactobacillus CICC 6075 was purchased from Beijing Biological Collection Center, and the plant lactobacillus ATCC 14917 used was purchased from Guangdong Microbiological Culture Collection Center. According to the method for preparing a facial cleanser with dual cleansing effects as described in claim 1, it is characterized in that: in the step S6, component C is prepared, 3 mL of natural extract fermentation liquid, 1.55 g of nano bamboo charcoal powder, 0.26 g of essence, 0.5 g of preservative and 1.25 g of citric acid are dissolved in 5 mL of deionized water, and stored for standby use; in the step S7, the A phase mixed component is quickly stirred at a stirring rate of 1200 r / min, and the B phase mixed component is pumped into the A phase mixed component in the emulsifying pot, and the mixed phase in the emulsifying pot is kept warm and stirred for 50 minutes at a temperature of 80°C to cause a saponification reaction.

7. A method for preparing a facial cleanser with double cleansing effects according to claim 1, Features: After the saponification reaction is completed in step S8, the mixture obtained in S3 is cooled to 45° C., and then the C component prepared by mixing according to its proportion is added thereto while stirring. After the addition is completed, it is continuously stirred at a rate of 400 r / min until the mixture in the emulsifier is naturally cooled to 36° C.

8. A facial cleanser with double cleansing effects prepared according to the preparation method according to any one of claims 1 to 9.

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