44results about How to "Stable survival" patented technology

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu/mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu/mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu/mL(or cfu/g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil/land and especially can be used for effectively improving soil polluted by heavy metals.

The invention discloses a fermented low-salt semi-dried tilapia mossambica, a processing method thereof and application thereof. The processing method comprises the steps of: removing the internal organs and the scales of the tilapia mossambica; cutting along backside or abdomen; overturning; flatly pressing to form a symmetrical tilapia mossambica piece except the tail of the tilapia mossambica;washing with cleaning water; washing with salt water with 1%-4% of concentration to remove fishy smell and obtain the tilapia mossambica piece without the fishy smell; paving salt on the surface of the tilapia mossambica piece without the fishy smell, wherein the weight of the salt is equal to 3-8% of that of the tilapia mossambica; sousing for 8-24 hours; drying; or soaking for 8-24 hours with 5-10% of salt water to obtain the low-salt tilapia mossambica piece; spraying zymophyte on the surface of the tilapia mossambica piece, wherein the inoculum size of the zymophyte is 105-107 CFU/g fish meat; fermenting for 10-48 hours in a sealing way under the temperature of 12-20 DEG C; and drying the fermented tilapia mossambica piece to obtain the fermented low-salt semi-dried tilapia mossambicawith 30-60% of water content. The product has lubricating meat quality and typical flavor of dried meat. The processing method can be adapted to processing freshwater fishes.

The invention discloses a blueberry SOD (Superoxide Dismutase) red wine and a preparation method thereof. The blueberry SOD red wine comprises 75ml of blueberry primary extraction solution, 100,000-200,000 units of blueberry SOD crude enzyme and 675ml of red wine. The preparation method of the blueberry SOD red wine comprises the following steps of: (1) cleaning blueberry; (2) draining water on the surface of the blueberry; (3) crushing and pulping by using a crushing and pulping machine, adding one time of volume of 4DEG C de-ionized water and carrying out ultrasound crushing at the constant temperature for 2 hours; (4) centrifuging to obtain supernate as the primary extraction solution; (5) intercepting the primary extraction solution to obtain a concentrated solution with the molecular weight of 20,000-60,000; (6) adding fucose as an SOD protecting agent and carrying out vacuum freezing and drying to obtain the blueberry SOD crude enzyme; and (7) mixing the blueberry SOD crude enzyme with the primary extraction solution, then adding the mixture into the red wine, sterilizing and bottling. The blueberry SOD wine obtained by the preparation method disclosed by the invention has the advantages of abundant nutrient components, favorable mouth feeling and high SOD survival rate.

The invention provides a liquid state composite microecological preparation for pigling and a preparation method thereof. Per milliliter of the liquid state composite microecological preparation contains lactic acid bacteria, whose live bacteria number is equal to or more than 2x107CFU, 30-60mg of Chitosan oligosaccharide, 0-60mg of vitamin C, 0-45mg of sodium butyrate and 0-60mg of L-glutamine. The preparation method comprises the followings steps: a MRS medium is used for cultivating lactic acid bacteria till the content of the target live bacteria is higher than or equal to 2x109CFU/ml, vitamin C, sodium butyrate, L-glutamine and Chitosan oligosaccharide are dissolved in a the microecological preparation liquid of the medium according to formula amount, a uniform mixing is carried out, and the composite microecological preparation liquid is obtained. According to characteristics of pigling intestinal flora growth and sucking pig breeding management, Chitosan oligosaccharide is used for enhancing stability of the microecological preparation, especially the stability of lactic acid bacteria, and a high efficiency liquid state composite microecological preparation is developed.

The invention belongs to the technical field of research and development of Vibrio alginolyticus phages and particularly relates to a wide-lytic-spectrum Vibrio alginolyticus phage, composition thereof, and a kit. The wide-lytic-spectrum Vibrio alginolyticus phage is Vibrio alginolyticus phage VAP7 collected under CCTCC NO: M 2018767. The inventio also discloses application of the Vibrio alginolyticus phage, a Vibrio alginolyticus phage composition, or a reagent or a kit containing the Vibrio alginolyticus phage or the Vibrio alginolyticus phage composition in the killing and/or prevention ofVibrio microbes. The Vibrio alginolyticus phage herein has a wide host range, has high toxicity to host bacteria under low concentration, can well propagate on nonpathogenic bacterial hosts, and is suitable for large-scale industrial production. Good strain resources can be provided for the application of phagotherapy.

The invention relates to a bacterial strain for preventing and treating soil-borne fungal diseases of peanuts in continuous cropping and a fungicide of the bacterial strain, wherein the bacterial strain is Pseudomonas protegens, and is assigned with the accession number of CGMCC No.11539. The 16s rRNA base sequence of the bacterial strain is shown as SEQ ID No:1. The bacterial strain has high activity in generating chitinase (degrading cell walls of pathogenic bacteria hyphae), and has the obvious degrading capacity for various soil-borne fungous pathogenic bacteria mycelia, such as fusarium, rhizoctonia and verticillium chlamydosporium. For the fungicide provided by the invention, the seed dressing use amount is only 20-30 kg/mu normally, the fungicide can be used for effectively preventing and treating the soil-borne fungous diseases, such as root rot, damping off and southern blight frequently occurring in peanuts continuously planted in dry fields, the control effect achieves 55% or above, in addition, the bacterial strain has the advantages of small use amount, wide antimicrobial spectrum, obvious yield-increasing effect and the like, so that the influence of the soil-borne diseases on the yield loss of the peanuts in continuous cropping is effectively alleviated.

The invention relates to the field of liquid milk, concretely, the invention relates to a colloid fluid suspension used for adding probiotics in acidic milk beverage and a method for producing probiotics-containing acidic milk beverage. According to the invention, the colloid fluid suspension used for adding aseptic probiotics in acidic milk beverage comprises the following components by weight, 1-5 parts of sugar and 0.1-0.6 parts of suspending agent; wherein the suspending agent comprises gellan gum, xanthan gum and acid resistant CMC according to a ratio of 1: 2 :3; 0.3-0.5 part of lactic acid and balance of water, wherein the pH value is 4.3-4.5. The method of the invention for producing probiotics-containing acidic milk beverage comprises the following steps: suspending aseptic embedded probiotics particles on the colloid fluid suspension, then ensuring asepsis and adding. According to the invention, probiotics stably survived in aseptic acidic milk beverage at normal temperaturecan be realized by the colloid fluid suspension, and probiotics is not easy to breed and grow in product shelf period, wherein the probiotics quantity in product is more than 107 cf umL, simultaneously the quality of the product can not be influenced, so that the active probiotics existed in the milk beverage product at normal temperature can be realized.

The invention discloses a compound inducing culture medium. The compound inducing culture medium contains three differentiation culture solutions AD, DB and DC. A method for inducing umbilical cord mesenchymal stem cells into neuron-like cells by virtue of the compound inducing culture medium comprises the following steps: pre-inducing the umbilical cord mesenchymal stem cells for 2 days by virtue of the differentiation culture solution DA; carrying out differentiation culture for 1 day by virtue of the differentiation culture solution DB; finally, carrying out maintenance culture for 1 day by virtue of the differentiation culture solution DC; and observing the morphological characteristics of the induced neuron-like cells, and detecting the mRNA level of induced neural differentiation relevant genes and the expression conditions of neuron migration proteins DCX and neuron specific enolase NSE which induce the neuron-like cells, wherein positive subjects of DCX expression are neural precursor cells, and positive subjects of NSE expression are the neuron-like cells. According to the method, the induction process is divided into three stages, and different culture mediums are adopted in each stage, so that the induction time is short, the induction efficiency is high, and the induced differentiated neuron-like cells can stably live and have no repellence after being transplanted.

The invention relates to a Paracoccus sp. With denitrification and phosphorus removal functions, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.19475. The screened Paracoccus sp. Has anaerobic denitrification and aerobic denitrification effects, and can be used for preparing a phosphorus removal agent. According to the present invention, the strain can efficiently remove the total nitrogen in the water body under the aerobic condition, can reduce the total phosphorus content in the wastewater, and can achieve the total phosphorus removal rate of 63.4% within 72 h when the bacterial liquid inoculation amount is 0.2 vol% and the initial phosphorus content is 13.4 mg/L.

The invention discloses an amino acid water-soluble fertilizer. The water-soluble fertilizer is prepared from paecilomyces lilacinus fermentation liquor, polyglutamic acid fermentation liquor, micromolecular amino acid, chitosan oligosaccharide, potassium alginate, chelated medium elements and a thickening agent. The product has the advantages that the fermentation liquor is directly used, so thatthe centrifuging or filtering process is avoided, and the cost is reduced; paecilomyces lilacinus is relatively good in nematode control effect, safe and free of pollution. The potash fertilizer increases the toughness of crop roots and can effectively prevent nematode infection; the micromolecular amino acid can be directly absorbed and utilized by the root system to promote rapid growth of thecrop root system or rapid recovery of the damaged root system; chitosan oligosaccharide is matched with organic matters, so that the influence of root-knot nematodes on crops can be reduced, and combined infection of bacteria or fungi on plant root systems is reduced. The microbial agent and the water-soluble fertilizer raw material can be mutually dissolved after being mixed, the solution is in astable colloid state, and conidia of the microbial agent can stably survive and keep biological activity for a long time.

The invention relates to an extraction method of an active factor for improving a survival rate of transplanted autologous fat such as autologous fat living cells, which comprises the following steps:1) putting extracted adipose tissue mixed liquid into a test tube, centrifuging, sucking supernatant plasma on the uppermost layer, adding a hydroxyethyl starch solution, and sufficiently and uniformly mixing, 2) adding into a nucleic acid type fermentation liquid, adding normal saline, stirring, standing, extracting fermentation liquid supernatant by using a sterile injector, adding a cryopreservation liquid into the fermentation liquid supernatant, and freezing by using semiconductor refrigeration equipment, (3) carrying out centrifugal operation by using a freezing centrifugal machine, after the fermentation liquor is liquefied again, adding the liquid after standing into a centrifugal tube, and carrying out secondary centrifugal treatment on the centrifugal tube in the centrifugal machine, 4) after centrifugal filtration, putting into a refrigerated centrifuge for centrifugal operation, and adding a new plasma sample to enable precipitated platelets to suspend again to obtain theactive factor. By means of the method, original adipose cells can be promoted to continue to grow and finally become adipose tissue stably, and the survival rate is high.

The invention discloses bacillus pumilus having functions of improving acid soil and decomposing potassium, and preparation and application of microbial inoculum of the bacillus pumilus. According tothe thallus shape, the colony characteristics and the physiological and biochemical properties of an HG-8 strain, through combination of the analysis result of 16S rDNA and gyrB sequences, the HG-8 strain is identified as the bacillus pumilus, the bacillus pumilus is preserved in China General Microbiological Culture Collection Center on 26 September 2019, and the preservation number is CGMCC NO.18620. The HG-8 strain can be stably colonized in the acid soil, the microbial inoculum prepared from the strain can be applied to improvement of growth of crops of wheat, corn, chilies and the like inthe acid soil, the pH value and the effective potassium content of the acid soil can be notably increased, and the yield of the crops can be increased. The strain has salt tolerance, besides, the fermentation properties of the strain are excellent, the microbial inoculum is simple in preparation technology, short in fermentation period and low in cost, and industrialized production and transportation are facilitated.

The invention belongs to the technical field of organoid preparation, and particularly relates to a preparation method of an organoid structure based on micro-fluidic technology high-throughput culture, which comprises a cell culture substrate, a micro-fluidic chip, a micro-fluidic control system, a high-throughput trace drug library modeling module and a micro-fluidic high-throughput drug screening module, the method specifically comprises the following steps: step 1, obtaining an organoid tissue sample, and cutting the organoid tissue sample into tissue blocks suitable for mechanical grinding; 2, the tissue blocks are ground through a mechanical grinding method, first-stage filtering is conducted through a first-stage filter screen with the pore diameter being 80-200 micrometers, and first-stage filtrate is formed; and step 3, carrying out second-stage filtration on the first-stage filtrate through a second-stage filter screen with the aperture of 30-60 [mu] m. The proliferation capacity of the organoid treated by the method is obviously higher than that of the organoid treated by an enzymolysis method, and the mechanical method can remarkably enrich tumor cells and remove normal cells, so that the tumor organoid with higher purity is obtained.

The invention discloses a method for improving fat survival rate and regeneration of adipose tissue, which includes extracting pre-adipocytes, transplanting fat cells, extracting nutrient components and backfilling and transplanting. The fat cells obtained by the method of the present invention can continuously differentiate into fat cells after transplantation, which solves the problems of traditional fat transplantation absorption activity and fat cell transplantation absorption or necrosis; the transplanted fat can survive in a long-term, lasting and stable manner. The material extracted by the present invention is transformed into fat cells after transplantation, which can promote the continuous growth of the original fat cells, and eventually become stable fat tissue, with a survival rate as high as 90%. Activated adipocytes transform into adipocytes, form adipose tissue at the site to be filled, promote the continued growth of original adipocytes, help form blood vessels, accelerate vascularization and resurvival of grafted fat, and release factors that promote angiogenesis, which can The rapid survival of transplanted fat into normal and viable adipose tissue can significantly improve the patient's physical beauty.