44results about How to "Stable survival" patented technology

The invention discloses a denitrifying strain with nitrifying function, a strain-containing water body improver of multiple active microorganisms and a preparation method of the water body improver, belonging to the technical fields of aquaculture and water body improvement. The strain disclosed by the invention is conserved in the China center for type culture collection (CCTCC), the CCTCC NO isM2011443; and the water body improver of the active microorganisms which is based on the strain comprises multiple strains such as Bacillus subtilis, Bacillus licheniformis, Bacillus pumilus, Bacillus cereus, Bacillus megaterium, Rhodopeudomonas palustris, Arthrobacter globiformis, Rhodotorula rubra and Lactobacillus plantarum. The strain is used to perform liquid fermentation and obtain fermentation liquor of which number of live bacteria is 0.5-3*10<10>cfu / mL, bacterial powder of which the number of live bacteria is 0.2-2*10<12>cfu / mL is further prepared; and the bacterial powder is used along with a diluent to prepare a liquid or powdery water body improver, wherein the effective live bacteria of the water body improver is 0.1-3*10<10>cfu / mL(or cfu / g). The water body improver has the function of reducing the contents of ammonia nitrogen, nitrites and nitrates in the culture water body; and the water body improver is suitable for different culture water bodies and has the function of improving the water quality.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

The invention discloses a fermented low-salt semi-dried tilapia mossambica, a processing method thereof and application thereof. The processing method comprises the steps of: removing the internal organs and the scales of the tilapia mossambica; cutting along backside or abdomen; overturning; flatly pressing to form a symmetrical tilapia mossambica piece except the tail of the tilapia mossambica;washing with cleaning water; washing with salt water with 1%-4% of concentration to remove fishy smell and obtain the tilapia mossambica piece without the fishy smell; paving salt on the surface of the tilapia mossambica piece without the fishy smell, wherein the weight of the salt is equal to 3-8% of that of the tilapia mossambica; sousing for 8-24 hours; drying; or soaking for 8-24 hours with 5-10% of salt water to obtain the low-salt tilapia mossambica piece; spraying zymophyte on the surface of the tilapia mossambica piece, wherein the inoculum size of the zymophyte is 105-107 CFU / g fish meat; fermenting for 10-48 hours in a sealing way under the temperature of 12-20 DEG C; and drying the fermented tilapia mossambica piece to obtain the fermented low-salt semi-dried tilapia mossambicawith 30-60% of water content. The product has lubricating meat quality and typical flavor of dried meat. The processing method can be adapted to processing freshwater fishes.

The invention relates to the field of microbes and provides a novel salmonella phage and a composition, a preparation method and application thereof. The novelsalmonella phage is Myoviridaesp. BP-66 with the preservation numberCCTCC NO: M2015146,Myoviridaesp. BP-63 with preservation number CCTCC NO: M2015145, or Chilikevirussp. BP-12 with the preservation number CCTCCNO: M2015141. The novel phage is a strict virulent phage, has high toxicity to host bacteria, has a wider host range and still has high toxicity to the host bacteria at low concentration. The DNA of the phage cannot encode protein possibly causing potential health risks. The novel phage stably survives in a culture solution at room temperature and survive for more than 6 months at the temperature below 4 DEG C. In addition, the phage can be proliferated on pathogenic bacterial hosts, and large-scale industrial production can be achieved. The salmonella phage can provide excellent strain resources for application of phage therapy.

The invention discloses a blueberry SOD (Superoxide Dismutase) red wine and a preparation method thereof. The blueberry SOD red wine comprises 75ml of blueberry primary extraction solution, 100,000-200,000 units of blueberry SOD crude enzyme and 675ml of red wine. The preparation method of the blueberry SOD red wine comprises the following steps of: (1) cleaning blueberry; (2) draining water on the surface of the blueberry; (3) crushing and pulping by using a crushing and pulping machine, adding one time of volume of 4DEG C de-ionized water and carrying out ultrasound crushing at the constant temperature for 2 hours; (4) centrifuging to obtain supernate as the primary extraction solution; (5) intercepting the primary extraction solution to obtain a concentrated solution with the molecular weight of 20,000-60,000; (6) adding fucose as an SOD protecting agent and carrying out vacuum freezing and drying to obtain the blueberry SOD crude enzyme; and (7) mixing the blueberry SOD crude enzyme with the primary extraction solution, then adding the mixture into the red wine, sterilizing and bottling. The blueberry SOD wine obtained by the preparation method disclosed by the invention has the advantages of abundant nutrient components, favorable mouth feeling and high SOD survival rate.

The invention discloses a lake eutrophication remediation method, which comprises the following steps of: (1) sequentially arranging a microbial remediation water tank, an absorption water tank and a cooling water tank on lakeshore in a half-burying way; (2) performing microbial remediation; (3) performing absorption; (4) performing cooling; and (5) performing ecological remediation in water. In the microbial remediation stage of the method, microorganisms grow stably in a certain controllable environment so as to achieve high microorganism survival rate; the microorganisms form a biomembrane system in a filler to achieve strain diversity; the microorganism system rapidly reduces the chemical oxygen demand (COD), biochemical oxygen demand (BOD) and ammonia nitrogen of organic pollutants without producing organic sludge and causing secondary pollutions; the method ensures high water purification dephosphorization efficiency; and exception handling for realizing a temperature reduction function is performed in the high temperature environment of a water bloom peak period.

The invention discloses a high-stability system enzymatic laundry soap composition and a production method thereof. The pH (Potential of Hydrogen) value of the high-stability system enzymatic laundry soap body disclosed by the invention is 7.0-9.5, and the soap body is obtained by compounding 50-98 parts of soap grain (sodium tallate soap), 0.01-5 parts of enzyme, 0.1-10 parts of a composite boron based stabilizer, 1-20 parts of a solvent, 0.01-8 parts of a pH regulating agent, 0.01-8 parts of a forming agent and 0.01-10 parts of other auxiliaries. The production method comprises the following steps of: completely dissolving the composite boron based stabilizer in the solvent, adding the enzyme into the obtained solution, sequentially mixing the soap grain, the forming agent, the pH regulating agent, enzyme mixture and other axillaries in a mixer, and producing the soap by means of laundry soap molding process. The enzyme activity of the enzymatic laundry soap provided by the invention can be kept above 30% within three-year quality guarantee period.

The invention belongs to the technical field of Escherichia coli, and particularly relates to a wide-lysis-spectrum Escherichia coli phage and a composition, a kit and application thereof. The phage is Escherichia coli phage CL1 which is collected under the number of CCTCC M 2018936. The phage is nonhazardous to normal microbial flora, resistant to high temperature and high in stability and has good application and development prospect, DNA of the phage cannot code virulence genes, and an excellent strain resource is provided for developing novel antibacterial preparations.

The invention provides a liquid state composite microecological preparation for pigling and a preparation method thereof. Per milliliter of the liquid state composite microecological preparation contains lactic acid bacteria, whose live bacteria number is equal to or more than 2x107CFU, 30-60mg of Chitosan oligosaccharide, 0-60mg of vitamin C, 0-45mg of sodium butyrate and 0-60mg of L-glutamine. The preparation method comprises the followings steps: a MRS medium is used for cultivating lactic acid bacteria till the content of the target live bacteria is higher than or equal to 2x109CFU / ml, vitamin C, sodium butyrate, L-glutamine and Chitosan oligosaccharide are dissolved in a the microecological preparation liquid of the medium according to formula amount, a uniform mixing is carried out, and the composite microecological preparation liquid is obtained. According to characteristics of pigling intestinal flora growth and sucking pig breeding management, Chitosan oligosaccharide is used for enhancing stability of the microecological preparation, especially the stability of lactic acid bacteria, and a high efficiency liquid state composite microecological preparation is developed.

The invention provides an aquatic-product compound amino acid bacterial fertilizer containing triacontanol and a preparation method thereof, and in particular provides an aquatic-product compound amino acid bacterial fertilizer containing triacontanol which can quickly cultivate algae at a low temperature, and can quickly form excellent water color within 2-3 days after sudden death of algae. Materials for preparing the compound amino acid bacterial fertilizer comprise triacontanol raw powder, alcohol, compound amino acid primary liquid, peregal, lauryl sodium sulfate, sodium silicate, zinc sulphate heptahydrate, ferrous sulfate, sodium molybdate, cobalt chloride, boric acid, manganese chloride and spore bacteria liquor. Free amino acid and each trace element in the aquatic-product compound amino acid bacterial fertilizer can be directly utilized by algae after being applied to a pond, so that conversion is quick and the algae photosynthesis is promoted; the low-concentration triacontanol can promote quick growth of beneficial algae such as diatom, green algae, and the like, at a low temperature, strengthen activity of the algae, so that fertilizer can quickly cultivate algae at a low temperature, and excellent water color can be quickly formed within 2-3 days after sudden death of algae.

The invention belongs to the technical field of research and development of Vibrio alginolyticus phages and particularly relates to a wide-lytic-spectrum Vibrio alginolyticus phage, composition thereof, and a kit. The wide-lytic-spectrum Vibrio alginolyticus phage is Vibrio alginolyticus phage VAP7 collected under CCTCC NO: M 2018767. The inventio also discloses application of the Vibrio alginolyticus phage, a Vibrio alginolyticus phage composition, or a reagent or a kit containing the Vibrio alginolyticus phage or the Vibrio alginolyticus phage composition in the killing and / or prevention ofVibrio microbes. The Vibrio alginolyticus phage herein has a wide host range, has high toxicity to host bacteria under low concentration, can well propagate on nonpathogenic bacterial hosts, and is suitable for large-scale industrial production. Good strain resources can be provided for the application of phagotherapy.

The invention relates to a bacterial strain for preventing and treating soil-borne fungal diseases of peanuts in continuous cropping and a fungicide of the bacterial strain, wherein the bacterial strain is Pseudomonas protegens, and is assigned with the accession number of CGMCC No.11539. The 16s rRNA base sequence of the bacterial strain is shown as SEQ ID No:1. The bacterial strain has high activity in generating chitinase (degrading cell walls of pathogenic bacteria hyphae), and has the obvious degrading capacity for various soil-borne fungous pathogenic bacteria mycelia, such as fusarium, rhizoctonia and verticillium chlamydosporium. For the fungicide provided by the invention, the seed dressing use amount is only 20-30 kg / mu normally, the fungicide can be used for effectively preventing and treating the soil-borne fungous diseases, such as root rot, damping off and southern blight frequently occurring in peanuts continuously planted in dry fields, the control effect achieves 55% or above, in addition, the bacterial strain has the advantages of small use amount, wide antimicrobial spectrum, obvious yield-increasing effect and the like, so that the influence of the soil-borne diseases on the yield loss of the peanuts in continuous cropping is effectively alleviated.

The invention relates to the field of liquid milk, concretely, the invention relates to a colloid fluid suspension used for adding probiotics in acidic milk beverage and a method for producing probiotics-containing acidic milk beverage. According to the invention, the colloid fluid suspension used for adding aseptic probiotics in acidic milk beverage comprises the following components by weight, 1-5 parts of sugar and 0.1-0.6 parts of suspending agent; wherein the suspending agent comprises gellan gum, xanthan gum and acid resistant CMC according to a ratio of 1: 2 :3; 0.3-0.5 part of lactic acid and balance of water, wherein the pH value is 4.3-4.5. The method of the invention for producing probiotics-containing acidic milk beverage comprises the following steps: suspending aseptic embedded probiotics particles on the colloid fluid suspension, then ensuring asepsis and adding. According to the invention, probiotics stably survived in aseptic acidic milk beverage at normal temperaturecan be realized by the colloid fluid suspension, and probiotics is not easy to breed and grow in product shelf period, wherein the probiotics quantity in product is more than 107 cf umL, simultaneously the quality of the product can not be influenced, so that the active probiotics existed in the milk beverage product at normal temperature can be realized.

The invention belongs to the technical field of microbial agents, and discloses a bacillus megaterium strain, and a preparation method and application thereof. The collection number of the bacillus megaterium strain is CCTCC (China Center for Type Culture Collection) M2018430. The preparation method for the bacillus megaterium strain comprises the following steps of: culture activation: carrying out activated culture on the bacillus megaterium preserved at a low temperature on an NA (Nutrient Agar) solid culture medium to obtain the activation seed of the bacillus megaterium; and culture seedsolution preparation: inoculating the bacillus megaterium obtained after activation is conducted into an NA liquid culture medium, and carrying out rejuvenation culture. The bacillus megaterium disclosed by the invention has the functions of stimulating crops to grow, regulating soil acility and alkalinity, accelerating soils to form a granular structure, inhibiting the growth of soil pathogenic bacteria and improving the micro-ecological environment of soils. By the application of the Bacillus megaterium strain YM11, the micro-ecological environment of a maize rhizosphere can be improved, rhizosphere nutrient element compositions are improved, the micro-ecological environment of the maize rhizosphere is changed, soil microbial diversity is increased, soil physicochemical properties are improved, and the yield of maize on a saline-alkali soil is increased.

The invention discloses a compound inducing culture medium. The compound inducing culture medium contains three differentiation culture solutions AD, DB and DC. A method for inducing umbilical cord mesenchymal stem cells into neuron-like cells by virtue of the compound inducing culture medium comprises the following steps: pre-inducing the umbilical cord mesenchymal stem cells for 2 days by virtue of the differentiation culture solution DA; carrying out differentiation culture for 1 day by virtue of the differentiation culture solution DB; finally, carrying out maintenance culture for 1 day by virtue of the differentiation culture solution DC; and observing the morphological characteristics of the induced neuron-like cells, and detecting the mRNA level of induced neural differentiation relevant genes and the expression conditions of neuron migration proteins DCX and neuron specific enolase NSE which induce the neuron-like cells, wherein positive subjects of DCX expression are neural precursor cells, and positive subjects of NSE expression are the neuron-like cells. According to the method, the induction process is divided into three stages, and different culture mediums are adopted in each stage, so that the induction time is short, the induction efficiency is high, and the induced differentiated neuron-like cells can stably live and have no repellence after being transplanted.

The invention relates to a Paracoccus sp. With denitrification and phosphorus removal functions, which is preserved in the China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC No.19475. The screened Paracoccus sp. Has anaerobic denitrification and aerobic denitrification effects, and can be used for preparing a phosphorus removal agent. According to the present invention, the strain can efficiently remove the total nitrogen in the water body under the aerobic condition, can reduce the total phosphorus content in the wastewater, and can achieve the total phosphorus removal rate of 63.4% within 72 h when the bacterial liquid inoculation amount is 0.2 vol% and the initial phosphorus content is 13.4 mg / L.

The invention discloses a bioreactor for treating high-salt organic wastewater based on a composite salt-tolerant bacterial strain. The bioreactor comprises a plurality of anaerobic reactors and aerobic sewage treatment devices which are sequentially communicated in series through communicating pipes, a supporting pore plate is fixedly arranged at the lower end in each anaerobic reactor, a fillerparticle bed layer is laid on each supporting pore plate, salt-tolerant anaerobic bacteria are added into each anaerobic reactor, and a first water distribution pipe is laid at the bottom in each anaerobic reactor; when every two adjacent anaerobic reactors are communicated in series through a communicating pipe fitting, a water filter screen is arranged at an inlet in the front end of the communicating pipe fitting and horizontally extends into the upper end of a previous anaerobic reactor, and an outlet in the rear end of the communicating pipe fitting is communicated with the first water distribution pipe laid at the bottom of the interior of a next anaerobic reactor; after anaerobic treatment, the wastewater flows into an aerobic sewage treatment device for aerobic treatment. The bioreactor disclosed by the invention is relatively high in high-salt organic wastewater treatment efficiency and low in wastewater treatment cost.

The invention discloses an amino acid water-soluble fertilizer. The water-soluble fertilizer is prepared from paecilomyces lilacinus fermentation liquor, polyglutamic acid fermentation liquor, micromolecular amino acid, chitosan oligosaccharide, potassium alginate, chelated medium elements and a thickening agent. The product has the advantages that the fermentation liquor is directly used, so thatthe centrifuging or filtering process is avoided, and the cost is reduced; paecilomyces lilacinus is relatively good in nematode control effect, safe and free of pollution. The potash fertilizer increases the toughness of crop roots and can effectively prevent nematode infection; the micromolecular amino acid can be directly absorbed and utilized by the root system to promote rapid growth of thecrop root system or rapid recovery of the damaged root system; chitosan oligosaccharide is matched with organic matters, so that the influence of root-knot nematodes on crops can be reduced, and combined infection of bacteria or fungi on plant root systems is reduced. The microbial agent and the water-soluble fertilizer raw material can be mutually dissolved after being mixed, the solution is in astable colloid state, and conidia of the microbial agent can stably survive and keep biological activity for a long time.

The invention relates to the field of microorganisms, and provides a novel salmonella phage, a composition thereof, and a preparation method and application thereof. The novel salmonella phage is a myoviridae BP-66, the preservation number is CCTCC NO: M2015146, the preservation number of the myoviridae BP-63 is CCTCC NO: M2015145, the preservation number of chilikevirus BP-12 is CCTCC NO: M2015141. The novel bacteriophage is a strict virulent bacteriophage, has high toxicity to host bacteria, has a wide host range, and still has high toxicity to the host bacteria at low concentration; the DNAof the phage cannot encode protein that can pose a potential health risk; the bacteriophage stably survive in a culture solution at room temperature, and can live for more than 6 months below 4 DEG C; the bacteriophage can be well proliferated on a non-pathogenic bacterial host; large-scale industrial production can be achieved. The salmonella phage can provide excellent strain resources for theapplication of phage therapy.

The invention discloses a blueberry SOD (Superoxide Dismutase) red wine and a preparation method thereof. The blueberry SOD red wine comprises 75ml of blueberry primary extraction solution, 100,000-200,000 units of blueberry SOD crude enzyme and 675ml of red wine. The preparation method of the blueberry SOD red wine comprises the following steps of: (1) cleaning blueberry; (2) draining water on the surface of the blueberry; (3) crushing and pulping by using a crushing and pulping machine, adding one time of volume of 4DEG C de-ionized water and carrying out ultrasound crushing at the constanttemperature for 2 hours; (4) centrifuging to obtain supernate as the primary extraction solution; (5) intercepting the primary extraction solution to obtain a concentrated solution with the molecularweight of 20,000-60,000; (6) adding fucose as an SOD protecting agent and carrying out vacuum freezing and drying to obtain the blueberry SOD crude enzyme; and (7) mixing the blueberry SOD crude enzyme with the primary extraction solution, then adding the mixture into the red wine, sterilizing and bottling. The blueberry SOD wine obtained by the preparation method disclosed by the invention has the advantages of abundant nutrient components, favorable mouth feeling and high SOD survival rate.

The present invention relates to anti-saline-alkali aspergillus ochratensis strain W1 and a micro-organism agent thereof, and applications of the anti-saline-alkali aspergillus ochratensis strain W1.The strain is aspergillus ochratensis preserved in China General Microbiological Culture Collection Center and has a preservation name of aspergillus ochratensis W1 and a preservation number of CGMCCNO.17971. The anti-saline-alkali aspergillus ochratensis strain W1 can stably survive in the soil with high sodium salt concentration and high pH, effectively degrades straws and is suitable for rebuilding saline-alkali land.

The invention discloses a device and a method for measuring dryness of gas-liquid two-phase fluid. The device comprises a cyclone, a measuring tube, a tracing bubble unit, an image acquisition unit, a differential pressure transmitter and an industrial personal computer, wherein the cyclone is mounted in the measuring tube and used for enabling the gas-liquid two-phase fluid to generate separating force between gas and liquid, the liquid phase flows along the inner wall of the measuring tube to form a liquid film, and the gas phase flows in the center of the measuring tube to form a gas core; the tracing bubble unit is used for planting a plurality of tracing bubbles into the liquid film; the differential pressure transmitter is used for measuring the pressure difference between the two ends of the cyclone; the image acquisition unit is used for acquiring images of a liquid film and a gas core in the measuring tube and an image of a motion trail of tracing bubbles, and the industrial personal computer is used for analyzing the images to obtain the flow velocity and the flow area of the liquid film and the flow of the liquid film, calculating the gas phase flow according to the flow of the liquid film and a pressure difference value, and finally determining a dryness value. The measurement process has the characteristics of visualization and good real-time performance.

The invention provides a microbial composition comprising acetobacter aceti, beauveria bassiana, azospirllum brasilense, nocardia cellulans, bacillus mucilaginosus, metarhizium anisopliae, trichoderma aureoviride and the like. The microbial composition can be stably combined in a microbial agent, is used for improving soil / land and especially can be used for effectively improving soil polluted by heavy metals.

The invention discloses enterobacter adami and application thereof in preventing and treating bacterial soft rot of plants. The collection number of the enterobacter adamsii L95 is GDMCCNO.62058. The enterobacter adamsii L95 can efficiently quench a specific VFM quorum sensing signal generated by Dickeya soft rot fungi, obviously reduces the occurrence of crop bacterial soft rot caused by pathogenic bacteria of the Dickeya, and has an excellent prevention and treatment effect in a pot experiment; and the strain can stably survive, is safe and does not have virogenic power. The invention provides a new biocontrol bacterium for safely and effectively preventing and treating bacterial soft rot of crops, and has a good application prospect.

The invention discloses treatment equipment for coating waste gas. The treatment equipment comprises an oxidizing chamber, and a first heat accumulation chamber, a second heat accumulation chamber and a third heat accumulation chamber which are communicated with the oxidizing chamber. The oxidizing chamber is provided with a heating device; each of the first heat accumulation chamber, the second heat accumulation chamber and the third heat accumulation chamber is provided with a gas inlet, a gas outlet and a hot gas inlet for suctioning hot gas; each of the first heat accumulation chamber, the second heat accumulation chamber and the third heat accumulation chamber is internally provided with a bottom heat accumulation layer, a middle heat accumulation layer and an upper heat accumulation layer; each gas outlet is connected with an air blower; the air blower is provided with an air outlet; and the air outlet is communicated with the hot air inlet through a pipeline. The treatment equipment for coating waste gas is simple in structure and low in maintenance cost; the heat accumulation chamber is reasonable in structure design and by the arrangement of multiple heat accumulation layers, heat is accumulated and stored stably and not liable to run away; and a filtering box is arranged in front of the heat accumulation chamber and use for filtering large-particle substances which can not be decomposed easily, piling and jamming of particle substances in the heat accumulation chamber are not liable to occur and then influences on the accumulation of heat are avoided.

The invention relates to an extraction method of an active factor for improving a survival rate of transplanted autologous fat such as autologous fat living cells, which comprises the following steps:1) putting extracted adipose tissue mixed liquid into a test tube, centrifuging, sucking supernatant plasma on the uppermost layer, adding a hydroxyethyl starch solution, and sufficiently and uniformly mixing, 2) adding into a nucleic acid type fermentation liquid, adding normal saline, stirring, standing, extracting fermentation liquid supernatant by using a sterile injector, adding a cryopreservation liquid into the fermentation liquid supernatant, and freezing by using semiconductor refrigeration equipment, (3) carrying out centrifugal operation by using a freezing centrifugal machine, after the fermentation liquor is liquefied again, adding the liquid after standing into a centrifugal tube, and carrying out secondary centrifugal treatment on the centrifugal tube in the centrifugal machine, 4) after centrifugal filtration, putting into a refrigerated centrifuge for centrifugal operation, and adding a new plasma sample to enable precipitated platelets to suspend again to obtain theactive factor. By means of the method, original adipose cells can be promoted to continue to grow and finally become adipose tissue stably, and the survival rate is high.

The invention discloses bacillus pumilus having functions of improving acid soil and decomposing potassium, and preparation and application of microbial inoculum of the bacillus pumilus. According tothe thallus shape, the colony characteristics and the physiological and biochemical properties of an HG-8 strain, through combination of the analysis result of 16S rDNA and gyrB sequences, the HG-8 strain is identified as the bacillus pumilus, the bacillus pumilus is preserved in China General Microbiological Culture Collection Center on 26 September 2019, and the preservation number is CGMCC NO.18620. The HG-8 strain can be stably colonized in the acid soil, the microbial inoculum prepared from the strain can be applied to improvement of growth of crops of wheat, corn, chilies and the like inthe acid soil, the pH value and the effective potassium content of the acid soil can be notably increased, and the yield of the crops can be increased. The strain has salt tolerance, besides, the fermentation properties of the strain are excellent, the microbial inoculum is simple in preparation technology, short in fermentation period and low in cost, and industrialized production and transportation are facilitated.

The invention belongs to the technical field of organoid preparation, and particularly relates to a preparation method of an organoid structure based on micro-fluidic technology high-throughput culture, which comprises a cell culture substrate, a micro-fluidic chip, a micro-fluidic control system, a high-throughput trace drug library modeling module and a micro-fluidic high-throughput drug screening module, the method specifically comprises the following steps: step 1, obtaining an organoid tissue sample, and cutting the organoid tissue sample into tissue blocks suitable for mechanical grinding; 2, the tissue blocks are ground through a mechanical grinding method, first-stage filtering is conducted through a first-stage filter screen with the pore diameter being 80-200 micrometers, and first-stage filtrate is formed; and step 3, carrying out second-stage filtration on the first-stage filtrate through a second-stage filter screen with the aperture of 30-60 [mu] m. The proliferation capacity of the organoid treated by the method is obviously higher than that of the organoid treated by an enzymolysis method, and the mechanical method can remarkably enrich tumor cells and remove normal cells, so that the tumor organoid with higher purity is obtained.

The invention discloses a method for improving fat survival rate and regeneration of adipose tissue, which includes extracting pre-adipocytes, transplanting fat cells, extracting nutrient components and backfilling and transplanting. The fat cells obtained by the method of the present invention can continuously differentiate into fat cells after transplantation, which solves the problems of traditional fat transplantation absorption activity and fat cell transplantation absorption or necrosis; the transplanted fat can survive in a long-term, lasting and stable manner. The material extracted by the present invention is transformed into fat cells after transplantation, which can promote the continuous growth of the original fat cells, and eventually become stable fat tissue, with a survival rate as high as 90%. Activated adipocytes transform into adipocytes, form adipose tissue at the site to be filled, promote the continued growth of original adipocytes, help form blood vessels, accelerate vascularization and resurvival of grafted fat, and release factors that promote angiogenesis, which can The rapid survival of transplanted fat into normal and viable adipose tissue can significantly improve the patient's physical beauty.

The invention discloses probiotics-containing jelly powder. The jelly powder is prepared from the following raw materials: powdered sugar, colloid, whey protein, fruit and vegetable powder, an acidulant and probiotics. The invention also discloses a method for preparing the jelly powder, which comprises the following steps of: mixing the powdered sugar, the acidulant and the colloid to prepare a mixture A; (2) crushing the mixture A to obtain a mixture B; (3) mixing the mixture B, whey protein and fruit and vegetable powder, drying and sieving to prepare a mixture C; and (4) uniformly mixing the mixture C with probiotics to obtain the jelly powder. The jelly powder has the advantages that the problem that thermosensitive substances cannot be added in the prior art is solved, the jelly powder capable of forming jelly only by adding warm water is provided for people, the prepared jelly powder is long in shelf life, probiotics can stably survive, and the prepared jelly thermosensitive substances are greatly reserved and smooth in taste.