The invention relates to an extraction method of an active factor for improving a
survival rate of transplanted
autologous fat such as
autologous fat living cells, which comprises the following steps:1) putting extracted
adipose tissue mixed liquid into a
test tube, centrifuging, sucking supernatant
plasma on the uppermost layer, adding a
hydroxyethyl starch solution, and sufficiently and uniformly mixing, 2) adding into a
nucleic acid type
fermentation liquid, adding normal
saline, stirring, standing, extracting
fermentation liquid supernatant by using a sterile
injector, adding a
cryopreservation liquid into the
fermentation liquid supernatant, and freezing by using
semiconductor refrigeration equipment, (3) carrying out centrifugal operation by using a freezing centrifugal
machine, after the fermentation liquor is liquefied again, adding the liquid after standing into a centrifugal tube, and carrying out secondary centrifugal treatment on the centrifugal tube in the centrifugal
machine, 4) after
centrifugal filtration, putting into a refrigerated
centrifuge for centrifugal operation, and adding a new
plasma sample to enable precipitated platelets to suspend again to obtain theactive factor. By means of the method, original adipose cells can be promoted to continue to grow and finally become
adipose tissue stably, and the
survival rate is high.