36 results about "Viable cell" patented technology

The present disclosure includes compounds, compositions, and methods for nucleic acid amplification reactions. In particular, the present disclosure provides compounds, compositions, and methods for viability PCR (vPCR) applications, in which the compounds that selectively bind to nucleic acids from non-viable cells.

The application belongs to the technical field of microorganism detection, and particularly relates to a bifidobacterium viable count detection culture medium, a detection method and application. The application provides a culture medium for detecting bifidobacterium, wherein the culture medium comprises MRS culture medium and an antibiotic solution; the antibiotic solution comprises streptomycin, kanamycin and sodium propionate. The culture medium can ensure normal growth of bifidobacterium, has strong inhibition on lactobacillus, can effectively realize accurate detection of bifidobacterium, and avoids interference of lactobacillus.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

Provided are methods of selecting a transformed cell, including methods comprising delivering an inhibitor of a protein to a plurality of cells, exposing the plurality of cells to a compound, wherein the protein (if present and active) converts the compound to a toxic compound, and selecting viable cells from the plurality of cells not killed by exposure to the compound.

Described are methods and systems for enhanced dissociation of cells from a solid biological tissue sample. In some embodiments, a self-contained cartridge apparatus includes a first chamber for receiving the tissue sample, enables ultrasonic energy from a transducer assembly of a processing unit to dissociate cells from the sample in the first chamber, and collect viable cells of interest from an aqueous suspension in a second chamber fluidly connected to the first chamber via a channel. In some embodiments, to enhance dissociation of viable cells, a filter device includes a tubular body configured to be telescopically inserted into a container containing the tissue sample in an aqueous fluid. The filter device also includes a cell-filter mesh that covers a bottom opening of the tubular body and that is configured to compress the tissue sample to expel cells from the sample when the filter device is fully inserted into the container.

The invention provides a complex microbial inoculant for promoting acid sugarcane soil improvement as well as a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The complex microbial inoculant comprises bacillus amyloliquefaciens N15300, bacillus megatherium 2020055 and bacillus licheniformis 2020049, wherein the ratio of the effective viable count of the bacillus amyloliquefaciens N15300 to the effective viable count of the bacillus megatherium 2020055 to the effective viable count of the bacillus licheniformis 2020049 is (1.8-2.2): (1.8-2.2): (0.8-1.2), and the total effective viable count is greater than or equal to 1 * 10 The complex microbial inoculant can effectively act on acidic sugarcane soil in places such as Guangxi, has multiple functions of dissolving phosphorus, dissolving potassium and secreting cellulase and protease, and can activate fixed nutrients in the soil, improve the utilization rate of a fertilizer, neutralize the acidity of the soil and improve the pH value of the soil. Pot experiments show that the bacterial agent can significantly promote sugarcane root development and plant growth, improve the fertilizer utilization rate, and provide an effective way for realizing high and stable yield of sugarcane and green improvement of acid soil.

The application discloses a safe, efficient and low-cost cleaning solution for suspended cells and application thereof, which comprises multiple kinds of glucose, amino acid supplements, vitamin supplements, anti-shear protective agents and sodium bicarbonate, is designed for the harvesting and washing process of suspended cells, can effectively remove residual culture medium, cell fragments, metabolic waste and process-related impurities while effectively maintaining cell viability, viable cell density and normal morphology, so as to realize the harvesting target of high-purity, high-activity and low-risk material of suspended cells, and is especially suitable for the production process of high-demand cell products such as cell culture meat. The cleaning solution system can be adapted to the continuous harvesting process of a large-scale bioreactor, can be integrated with automatic harvesting equipment, realizes stable, efficient and repeatable cell collection and washing operation, and meets the full-scene requirements from laboratory research and development to industrial production.

Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells. Some methods comprise dividing the sample into two or more sub-samples or sample portions, mixing each sub-sample or sample portion with one or more reagents and / or one or more reactants forming distinct sub-sample or sample portion mixtures, compartmentalizing each of the sub-sample or sample portion mixtures into a plurality of small volume compartments, monitoring characteristics of the small volume compartments over time and collecting compartment data, and transmitting the collected data to at least one neural network.

The invention relates to a pesticide-fertilizer granule and a preparation method thereof, the granule is composed of a compound functional flora domesticated by saline-alkali gradient stress, an organic carrier prepared by fermenting agricultural and forestry wastes and livestock and poultry manure, and a chemical synergistic component, and the effective viable count is greater than or equal to 0.5 billion CFU / g. The preparation method comprises the following steps: (1) screening salt-dissolving, growth-promoting and stress-resistant composite flora from a saline-alkali soil sample; (2) performing high-temperature aerobic fermentation on forestry and agricultural residues and livestock and poultry manure to prepare a carrier; (3) inoculating the functional flora to the carrier by adopting a segmented inoculation process; and (4) extruding and granulating at a low temperature lower than 0 DEG C. The bottleneck that a traditional bacterial fertilizer is single in function, high in carrier cost, large in granulation viable bacterium loss and the like is broken through, a chemical modifier and bacterial fertilizer synergistic application mode is innovated for severe saline-alkali soil, the soil salt content is reduced by 25%-30%, the crop yield is increased by 15%-20%, the strain survival rate is larger than or equal to 75%, and a standard and low-cost solution is provided for green improvement of the saline-alkali soil.

The invention discloses a fermentation method and application of bacillus laterosporus, and belongs to the technical field of microbial fermentation engineering and biological control. In order to solve the technical problems of unstable fermentation level, low spore formation rate and the like of bacillus laterosporus, the invention provides a high-density fermentation method of bacillus laterosporus, which comprises the following steps: adding a nutrition-signal composite inducer in stages in the middle and later periods of fermentation, and coupling pH / DO two-stage control, so that the spore formation rate is greater than or equal to 95%; besides, fermentation liquor of bacillus laterosporus and the bentonite-trehalose-sodium glutamate composite carrier are mixed and then subjected to low-temperature spray drying, the viable bacterium yield of the prepared microbial agent is larger than or equal to 95%, the viable bacterium rate is larger than or equal to 90% after the microbial agent is stored for 12 months at the temperature of 25 DEG C, and the microbial agent shows a remarkable effect in tomato root-knot nematode prevention and treatment of livestock and poultry diarrhea and has good application prospects. The compound can completely or partially replace chemical pesticides and antibiotics, and has a wide market prospect.

Provided herein is a composition comprising a hydrogel covalently bound to a functional moiety, wherein the hydrogel comprises a cross linkable biopolymer and a first cell population; the cross linkable biopolymer is at least partially crosslinked by a crosslinking agent within the hydrogel; and the functional moiety has a binding affinity to a cell. Furthermore, a method of growing cells using the composition of the invention, and a method of manufacturing the composition using a kit disclosed herein are also provided.

Embodiments of the present disclosure provide compositions and methods for injectable recombinant protein-based hydrogels for therapeutic delivery. Embodiments of the composition and methods comprise a recombinant protein, a cell consortia, and a biocompatible medium. The recombinant protein comprises one or more self-association domains and a flexible linker comprising a pseudorepeat of amino acids, forming stable hydrogels in contact with the cell consortia. Such composition and methods have broad application in delivery of viable cells to a biological tissue for tissue repair and regeneration.

In certain embodiments, this disclosure provides novel methods of increasing the viable cell density of an N−1 large-scale bioreactor cell culture, comprising culturing a host cell expressing a recombinant poly peptide of interest in a non-perfusion-based culture system, and wherein the viable cell density is increased to at least 5×106 cells / mL. In certain embodiments, the disclosure provides novel methods for large-scale production of a recombinant polypeptide of interest, comprising: (1) culturing a host cell expressing a recombinant polypeptide of interest in an N−1 stage in a non-perfusion-based culture system, wherein the viable cell density is increased to at least 5×106 cells / mL; and (2) culturing N fed-batch production cells in an enriched media with high-seed density at least 1.5×106 cells / mL, wherein the N fed-batch production cells are inoculated from the N-1 stage in a non-perfusion-based culture system.

Provided are a decellularized amniotic membrane matrix hydrogel and its preparation method, which includes the following steps: pre-treatment of an amniotic membrane tissue, washing, decellularization, grinding, freeze-drying, digestion, and acid-base neutralization processes. The decellularized amniotic membrane matrix hydrogel provided in this disclosure retains components such as collagen, fibronectin, and glycoproteins from the amniotic membrane while removing viable cells, thereby reducing its immunogenicity at the source. The decellularized amniotic membrane matrix hydrogel may mimic the microenvironment in which cells exist in vivo, providing the necessary support and signals for cell adhesion and growth. It can be used to construct tissue engineering scaffolds, supporting directed cell growth and tissue regeneration. Furthermore, the decellularized amniotic membrane matrix hydrogel may also serve as a drug carrier for in vivo drug release and therapy, demonstrating significant potential for application in medical research and clinical settings.

This invention discloses a method for sensitively detecting water toxicity using CHO-k1 cell fluorescence and its application, belonging to the field of water safety detection technology. The method uses CHO-k1 cells as the test carrier. After cell preparation, solid-phase extraction enrichment of water samples, and exposure to toxic substances, the method simultaneously detects cell inhibition rate and intracellular reactive oxygen species (ROS) fluorescence intensity. The total fluorescence intensity is corrected by the number of surviving cells, and the relative ROS fluorescence intensity and oxidative stress impact rate (R) of a single cell are calculated to establish a four-level toxicity grading standard, enabling quantitative evaluation of water sample toxicity. The detection sensitivity of this method is far higher than traditional cytotoxicity detection methods. It can detect sub-lethal damage from low concentrations of pollutants, and the results are accurate and reliable. The operation is standardized and applicable to rapid toxicity screening and water environment health risk assessment of various water samples, including surface water, groundwater, municipal water supply, industrial wastewater, and reclaimed water.

The present disclosure provides, among other things, continuous culture methods for producing a cell product, e.g., a recombinant protein, e.g., a glycoprotein, e.g., an antibody agent or a fusion protein. In some instances, methods herein allow large-scale production of a recombinant protein using continuous culture methods. The present disclosure identifies and addresses a problem with current continuous cell culture techniques in that at large-scale culture of certain cells have insufficient viable cell concentrations and impaired cell viability. The present disclosure provides, in part, methods and systems for large-scale continuous culture of shear-sensitive cells.

The invention relates to a culture medium for culturing human mycoplasma pneumoniae and a culture method, and belongs to the technical field of microorganisms, the culture medium for culturing the human mycoplasma pneumoniae comprises a mycoplasma culture medium and a growth promoting additive, the growth promoting additive comprises horse serum or fetal calf serum, cholesterol, penicillin sodium and an HEPES buffer solution, according to the culture medium for culturing the human mycoplasma pneumoniae, various components are simple and easy to obtain, the preparation method of the culture medium is greatly simplified, and the efficiency is improved; according to the culture method of the human mycoplasma pneumoniae, the culture mode is changed from a static state to a dynamic state, so that the culture efficiency of the human mycoplasma pneumoniae is improved, and meanwhile, the thallus yield of the human mycoplasma pneumoniae is also improved; the culture method for preparing the human mycoplasma pneumoniae is short in period, low in cost, high in culture viable organism density and high in yield, and is suitable for large-scale culture of the human mycoplasma pneumoniae.

Provided are: a cell aggregate containing adhesive cells and an extracellular matrix, and also containing survival cells at a depth of 30 [mu] m or more from the surface of the aggregate; disclosed is a method for producing a cell aggregate, which comprises supporting adhesive cells on a molded body that is formed from fibers containing an ethylene-vinyl alcohol copolymer and has a non-woven fiber structure, and culturing the cells while stirring.

The present disclosure relates to methods of treating triple negative breast cancer or non-muscle invasive bladder cancer in a subject comprising administering to the subject a composition comprising non-viable cells of Streptococcus pyogenes and an immune checkpoint inhibitor.

The invention provides a feeder cell density and motility rate detection kit and a detection method thereof, and belongs to the technical field of cell detection. The invention specifically discloses a method for detecting cell density and / or motility rate, which comprises the following steps: (1) exposing feeder cells to a CFSE dye environment for dyeing; (2) removing the CFSE dye in the environment; (3) culturing the feeder cells under proper conditions; (4) adding absolute counting microspheres into the cultured feeder cells and exposing the feeder cells in a dead cell dye environment for dyeing; and (5) calculating the density or the motility rate of the living cells according to a dyeing result and absolute counting microspheres. The method has good specificity, accuracy, precision and durability, and has good popularization value and market application prospect.

The invention provides probiotic whole-wheat bread with high viable organism retention rate and a preparation method thereof. The method comprises the following steps: firstly, carrying out osmotic pressure shock pretreatment on probiotics, mixing with a carbohydrate-protein compound solution, coating by using a fluidized bed to form a thermal protection layer, drying, and then spraying a hydrophobic lipid material to obtain multiple embedded particles; blending the whole wheat flour, the yeast and the water to form a dough, and adding the particles 3-5 minutes before the stirring is finished; and fermenting at 35-38 DEG C and 75-85% RH for 60-90 minutes, and baking at 160-190 DEG C for 15-25 minutes to obtain the finished product. According to the method, the probiotics can be protected, the high viable bacteria retention rate is kept in the bread baking and normal-temperature storage process, the functionality of the probiotic bread is improved, and the shelf life of the probiotic bread is prolonged.

This application relates to a biological tissue grading and screening device, comprising a mixed-flow screening module and a push pump assembly. The mixed-flow screening module further includes a first mixed-flow unit, at least one second mixed-flow unit and a third mixed-flow unit connected in series, and at least one grading filter. The push pump assembly includes a sample push pump and at least two collection push pumps. The sample push pump is connected to the inlet end of the first mixed-flow unit, and the first collection push pump of the at least two collection push pumps is connected to the outlet end of the third mixed-flow unit. The remaining collection push pumps are each connected to the outlet end of a grading filter. This biological tissue grading and screening device employs a purely physical separation technology of push-mixed flow and grading filtration. Different separation programs can be set for different biological tissues and cell types to obtain target samples of different grades. It not only achieves high cell recovery rate and cell purity but also improves the viable cell rate.

An improved packaging assembly for storing, distributing, treating, mixing, and dispensing tissue and / or cellular material and / or implantable material. The packaging assembly may include pouches, tubes, and a bag made of a sealable, flexible polymeric material that is open at one end and a needle-free swabable connector attached to the pouch at the other end and acting as a port to allow for the introduction or discharge of biological solutions, rinsing solution, and / or preservation solutions into the packaging assembly. The designed thickness of the wall of the packaging assembly facilitates efficient heat / cold transfer, which is useful for successful controlled rate freezing, quick thawing, and resuscitation of viable cells or tissue. The packaging assembly is also useful for combining additional biological fluids with the cellular material or tissue, and for efficient mixing of the biological fluids with the tissue and / or cellular material in the assembly.

The present disclosure relates to methods of treating triple negative breast cancer or non-muscle invasive bladder cancer in a subject comprising administering to the subject a composition comprising non-viable cells of Streptococcus pyogenes and an immune checkpoint inhibitor.

The invention relates to the technical field of food microorganisms, in particular to a high-activity probiotic double-layer tablet with a decayed tooth resisting function and a preparation method of the high-activity probiotic double-layer tablet. According to the probiotic double-layer tablet, fructo-oligosaccharide is further compounded on the basis of strain screening and compounding, the strain activity is better maintained, and then the decayed tooth resisting effect is enhanced. On the basis of probiotics and prebiotics, natural plant extracts are scientifically matched, streptococcus mutans are better inhibited, and the decayed tooth resisting effect is further enhanced. Meanwhile, the stability of the probiotic double-layer tablet is inspected, the viable count is more than 1 billion CFU / g at 20 DEG C for 12 months, and the viable survival rate is more than 35%. The obtained probiotic double-layer tablet is high in pressure resistance, good in stability, high in probiotic survival rate, low in product cost and beneficial to large-scale industrial production.

The present invention relates to a Bacillus velezensis SCB88 strain having excellent growth ability and maturation-promoting function in a starfish liquid fertilizer environment, and a composition containing the same. The above-mentioned SCB88 strain is a strain isolated from a specific environment and, when compared to other Bacillus velezensis strains (SCB64, SCB92) belonging to the same species, exhibits excellent proliferation ability, improved maturity, and increased fertilizer components (N, P, K) within starfish liquid fertilizer. In particular, this strain significantly increases the number of viable cells after inoculation into liquid fertilizer, improves the maturity to the 'completed maturity' stage, and significantly increases the content of major components such as nitrogen, phosphorus, and potassium, thereby demonstrating a very high potential for use as a functional probiotic. Furthermore, by experimentally proving that functional characteristics can vary depending on the source of isolation of the strain even within the same species, this invention emphasizes the importance of strain selection and source in the development of microorganism-based liquid fertilizer or fertilizer compositions.

The application provides a preparation method and application of a composite microbial agent. The composite microbial agent comprises Metarhizium anisopliae Metarhizium anisopliae ), Bacillus agri Achromobacter sp. ) and Bacillus proteolyticus Bacillus proteolyticus ), and the ratio of the viable cell numbers of the Metarhizium anisopliae Metarhizium anisopliae ), Bacillus agri Achromobacter sp. ) and Bacillus proteolyticus Bacillus proteolyticus ) in the composite microbial agent is 0.5-1.5:0.5-1.5:0.5-1.5. The compost prepared by using the microbial agent as a microbial strain can significantly improve the composting speed, reduce the biological toxicity of the compost product, increase the nutrient retention of the compost product, improve the nutrient content and safety of the compost product, and effectively improve the soil organic matter, total nitrogen nutrient content and potato quality by adding the microbial fertilizer prepared by adding the composite microbial agent.

This invention discloses a high-purity, multifunctional probiotic powder and its preparation method. Based on the different characteristics of lactic acid bacteria, Bacillus subtilis, and actinomycetes, this invention employs different methods for their respective proliferation. This not only avoids potential mutual interference and contamination during their individual proliferation processes but also achieves high viable cell counts while maintaining high purity, and the high viable cell count is maintained even after mixing. This invention mixes the proliferated bacterial powders in a reasonable ratio, allowing lactic acid bacteria to be the dominant strain while fully utilizing the functional characteristics of each of the three bacteria: lactic acid bacteria are anaerobic, while Bacillus subtilis and actinomycetes are aerobic. The latter can absorb oxygen released by the former, ensuring that in the anaerobic environment of the human intestine, Bacillus subtilis and actinomycetes remain active rather than dormant, generating resistant endospores through synergistic action with lactic acid bacteria.