95 results about "Viable cell" patented technology

The present disclosure includes compounds, compositions, and methods for nucleic acid amplification reactions. In particular, the present disclosure provides compounds, compositions, and methods for viability PCR (vPCR) applications, in which the compounds that selectively bind to nucleic acids from non-viable cells.

The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.

The invention relates to a target cell in-vitro sorting method for cell therapy, and belongs to the technical field of cell therapy. According to the method, the cleaning solution containing EGTA, EDTA, alpha-casein and prostaglandin is used, so that the sorting method with high living cell recovery efficiency is obtained; and cell therapy can be better realized.

The present invention relates to a method for in vitro sorting of target cells for cell therapy, belonging to the technical field of cell therapy. The present invention utilizes a washing solution containing EGTA, EDTA, α-casein, and prostaglandins to achieve a sorting method with high viable cell recovery efficiency, thereby enabling better cell therapy.

Methods for distinguishing particles in a fluid sample are disclosed. In one embodiment, the method includes acquiring a background SIMI image and a background brightfield image of a membrane filter while the membrane filter is free of a fluid sample, introducing a fluid sample onto the membrane filter, acquiring a SIMI image and a brightfield image of filtered particles resting on the membrane filter, distinguishing between the filtered particulates and the membrane filter based on the background SIMI image, generating a particle mask based on the SIMI image, and detecting beads via the particle mask. Methods for distinguishing particulates include distinguishing between viable and non-viable cell populations, distinguishing between cellular and non-cellular particulates, distinguishing between biological and non-biological particulates, distinguishing between first and second protein types, determining stability of monoclonal antibody drugs, identifying beads in a cell therapy, and detecting bacteria.

The application discloses a high Bacillus altitudinis SW2, belongs to the field of degrading bacteria screening, and the high Bacillus altitudinis SW2 has been preserved in the China General Microbiological Culture Collection Center on May 8, 2024, has a preservation number of CGMCC No.30525, and has a preservation address of No.3, Xili, Beichen West Road, Chaoyang District, Beijing City; is classified and named as the high Bacillus altitudinis. The strain is obtained through screening, purification and identification technologies from grape branch compost, and through research on strain characteristics, fermentation conditions and cellulase activity of the strain, it is shown that the enzyme activity of the strain is 547.82 U / g under the condition of 30 DEG C and PH=7, and when 5% casein is added as a protective agent, the effective viable cell number of the high Bacillus altitudinis SW2 is the highest and can reach 1.73*10 14 cfu / g.

The invention discloses a bacillus amyloliquefaciens strain and application thereof. The bacillus amyloliquefaciens SF-00595 disclosed by the invention is obtained by carrying out heat resistance domestication on bacillus amyloliquefaciens SDF-005, the preservation number of the bacillus amyloliquefaciens SF-00595 is CCTCC (China Center For Type Culture Collection) NO: M 2025830, and the bacillus amyloliquefaciens SF-00595 can be used as a biopesticide for preventing and treating papaya anthracnose, fig anthracnose and the like. The bacillus amyloliquefaciens SF-00595 strain is subjected to heat resistance domestication, so that the death rate of the strain during spray drying of fermentation liquor is reduced, and the content of viable bacteria in bacterial powder is increased.

Materials and methods for controlling nutrient feed in cell culture processes are provided. A sample is received from a bioreactor comprising a cell culture. A viable cell density and a residual nutrient measurement are determined from the received sample. A daily nutrient feed target is calculated based on the viable cell density and the residual nutrient measurement. The nutrient is fed to the bioreactor according to the calculated daily nutrient feed target.

The application belongs to the technical field of microorganism detection, and particularly relates to a bifidobacterium viable count detection culture medium, a detection method and application. The application provides a culture medium for detecting bifidobacterium, wherein the culture medium comprises MRS culture medium and an antibiotic solution; the antibiotic solution comprises streptomycin, kanamycin and sodium propionate. The culture medium can ensure normal growth of bifidobacterium, has strong inhibition on lactobacillus, can effectively realize accurate detection of bifidobacterium, and avoids interference of lactobacillus.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

Provided are methods of selecting a transformed cell, including methods comprising delivering an inhibitor of a protein to a plurality of cells, exposing the plurality of cells to a compound, wherein the protein (if present and active) converts the compound to a toxic compound, and selecting viable cells from the plurality of cells not killed by exposure to the compound.

Described are methods and systems for enhanced dissociation of cells from a solid biological tissue sample. In some embodiments, a self-contained cartridge apparatus includes a first chamber for receiving the tissue sample, enables ultrasonic energy from a transducer assembly of a processing unit to dissociate cells from the sample in the first chamber, and collect viable cells of interest from an aqueous suspension in a second chamber fluidly connected to the first chamber via a channel. In some embodiments, to enhance dissociation of viable cells, a filter device includes a tubular body configured to be telescopically inserted into a container containing the tissue sample in an aqueous fluid. The filter device also includes a cell-filter mesh that covers a bottom opening of the tubular body and that is configured to compress the tissue sample to expel cells from the sample when the filter device is fully inserted into the container.

The present invention relates to Lactobacillus paracasei HH20 MXS_B2_71_3 strain DSM 34860 and / or Lactobacillus rhamnosus HH03 OB-Lb-0655 strain DSM 34859, in the form of viable cells and / or non-viable cells and / or cell fragments and / or as fermentation broth, or combinations thereof, for use as a medicament, preferably for use as a medicament in one or more human and / or animal subject; as well as oral care compositions, methods and kits comprising said species.

The invention relates to a compound microbial agent and application thereof in repairing or controlling chlorinated hydrocarbon contaminated sites, the compound microbial agent comprises compound microorganisms, and the compound microorganisms comprise Gordonia, pseudomonas, pseudoxanthomonas and geomonas in a viable count ratio of 100: (25-600): (10-900): (10-500). The method effectively solves the problem that the chlorohydrocarbon contaminated site is difficult to repair, overcomes the problems of low mass transfer efficiency, limited microbial activity and the like of the traditional repair technology, improves the repair and risk management and control efficiency of the chlorohydrocarbon contaminated site, and reduces the construction cost. The method is especially suitable for green and efficient remediation of soil and underground water in silt soil sites, pollution source reduction and risk management and control, has the advantages of low cost, convenience in operation and the like, and has important application prospects.

To provide RNAi agents for inhibiting expression of the α-1 antitrypsin (AAT) gene, compositions including the AAT RNAi agents, and methods of using the same.SOLUTION: Also there are disclosed pharmaceutical compositions including one or more AAT RNAi agents together with one or more excipients capable of delivering the RNAi agents to living cells in vivo. The delivery of the AAT RNAi agents to liver cells in vivo inhibits AAT gene expression and treats diseases associated with AAT deficiency such as chronic hepatitis, cirrhosis, hepatocellular carcinoma, transaminitis, cholestasis, fibrosis, and fulminant hepatic failure.SELECTED DRAWING: Figure 9

The method for preparing cells overexpressing exogenous genes in the present invention specifically relates to the application of cGAS-STING signaling pathway inhibitors in culturing electroporated cells, the method for culturing electroporated cells, the method for preparing cells overexpressing exogenous genes by electroporation, and a cell culture medium supplemented with cGAS-STING signaling pathway inhibitors. By culturing the electroporated cells, especially immune effector cells, with a culture medium containing cGAS-STING signaling pathway inhibitors, the present invention can significantly reduce the mortality rate of the electroporated cells and increase the number and proportion of viable cells after cell electroporation.

The invention provides a complex microbial inoculant for promoting acid sugarcane soil improvement as well as a preparation method and application thereof, and belongs to the technical field of agricultural microorganisms. The complex microbial inoculant comprises bacillus amyloliquefaciens N15300, bacillus megatherium 2020055 and bacillus licheniformis 2020049, wherein the ratio of the effective viable count of the bacillus amyloliquefaciens N15300 to the effective viable count of the bacillus megatherium 2020055 to the effective viable count of the bacillus licheniformis 2020049 is (1.8-2.2): (1.8-2.2): (0.8-1.2), and the total effective viable count is greater than or equal to 1 * 10 The complex microbial inoculant can effectively act on acidic sugarcane soil in places such as Guangxi, has multiple functions of dissolving phosphorus, dissolving potassium and secreting cellulase and protease, and can activate fixed nutrients in the soil, improve the utilization rate of a fertilizer, neutralize the acidity of the soil and improve the pH value of the soil. Pot experiments show that the bacterial agent can significantly promote sugarcane root development and plant growth, improve the fertilizer utilization rate, and provide an effective way for realizing high and stable yield of sugarcane and green improvement of acid soil.

The application discloses a safe, efficient and low-cost cleaning solution for suspended cells and application thereof, which comprises multiple kinds of glucose, amino acid supplements, vitamin supplements, anti-shear protective agents and sodium bicarbonate, is designed for the harvesting and washing process of suspended cells, can effectively remove residual culture medium, cell fragments, metabolic waste and process-related impurities while effectively maintaining cell viability, viable cell density and normal morphology, so as to realize the harvesting target of high-purity, high-activity and low-risk material of suspended cells, and is especially suitable for the production process of high-demand cell products such as cell culture meat. The cleaning solution system can be adapted to the continuous harvesting process of a large-scale bioreactor, can be integrated with automatic harvesting equipment, realizes stable, efficient and repeatable cell collection and washing operation, and meets the full-scene requirements from laboratory research and development to industrial production.

Certain embodiments of the invention are directed to evaluating and identifying cells by recording and interpreting a time-dependent signal produced by unique cell respiration and permeability attributes of isolated viable cells. Some methods comprise dividing the sample into two or more sub-samples or sample portions, mixing each sub-sample or sample portion with one or more reagents and / or one or more reactants forming distinct sub-sample or sample portion mixtures, compartmentalizing each of the sub-sample or sample portion mixtures into a plurality of small volume compartments, monitoring characteristics of the small volume compartments over time and collecting compartment data, and transmitting the collected data to at least one neural network.

Multi-stage sample-recovery systems, including automated 2-stage and 3-stage sample-recovery systems, are provided. Such systems enable the rapid screening and recovery of samples, including viable cell-based samples, from high-throughput screening systems, including systems utilizing large-scale arrays of microcapillaries. In specific screening systems, each microcapillary comprises a solution containing a variant protein, an immobilized target molecule, and a reporter element. Immobilized target molecules may include any molecule of interest, including proteins, nucleic acids, carbohydrates, and other biomolecules. The association of a variant protein with a molecular target is assessed by measuring a signal from the reporter element. The contents of microcapillaries identified in the assays as containing variant proteins of interest can be identified and recovered using the multi-stage systems disclosed herein.

The invention relates to a preservative as well as a preparation method and application thereof. The preservative comprises a bacillus velezensis microbial inoculum and an elicitor cooperating with the microbial inoculum, and the addition amount of the elicitor is 0.01-0.1% of the total mass of the preservative. The preservative further comprises at least one of a protective agent, a preservative and an antioxidant; the bacillus velezensis microbial inoculum comprises the following components in parts by mass: 20-80 parts of a bacillus velezensis microbial inoculum, 0.001-0.1 part of an elicitor, 1-25 parts of a protective agent, 0.05-1 part of a preservative and 0.05-1 part of an antioxidant. The effective viable count in the bacillus velezensis microbial inoculum is greater than or equal to 10 < 9 > CFU / mL; the bacillus velezensis microbial inoculum comprises at least one of bacillus velezensis strains LTS-AF1, LTS-AF2, LTS-AF3 and LTS-AF4, and the bacillus velezensis microbial inoculum comprises at least one of bacillus velezensis strains LTS-AF1, LTS-AF2, the strain action effect is improved, the action time is prolonged, and the strain activity is stabilized.

The invention relates to a pesticide-fertilizer granule and a preparation method thereof, the granule is composed of a compound functional flora domesticated by saline-alkali gradient stress, an organic carrier prepared by fermenting agricultural and forestry wastes and livestock and poultry manure, and a chemical synergistic component, and the effective viable count is greater than or equal to 0.5 billion CFU / g. The preparation method comprises the following steps: (1) screening salt-dissolving, growth-promoting and stress-resistant composite flora from a saline-alkali soil sample; (2) performing high-temperature aerobic fermentation on forestry and agricultural residues and livestock and poultry manure to prepare a carrier; (3) inoculating the functional flora to the carrier by adopting a segmented inoculation process; and (4) extruding and granulating at a low temperature lower than 0 DEG C. The bottleneck that a traditional bacterial fertilizer is single in function, high in carrier cost, large in granulation viable bacterium loss and the like is broken through, a chemical modifier and bacterial fertilizer synergistic application mode is innovated for severe saline-alkali soil, the soil salt content is reduced by 25%-30%, the crop yield is increased by 15%-20%, the strain survival rate is larger than or equal to 75%, and a standard and low-cost solution is provided for green improvement of the saline-alkali soil.

The invention discloses a fermentation method and application of bacillus laterosporus, and belongs to the technical field of microbial fermentation engineering and biological control. In order to solve the technical problems of unstable fermentation level, low spore formation rate and the like of bacillus laterosporus, the invention provides a high-density fermentation method of bacillus laterosporus, which comprises the following steps: adding a nutrition-signal composite inducer in stages in the middle and later periods of fermentation, and coupling pH / DO two-stage control, so that the spore formation rate is greater than or equal to 95%; besides, fermentation liquor of bacillus laterosporus and the bentonite-trehalose-sodium glutamate composite carrier are mixed and then subjected to low-temperature spray drying, the viable bacterium yield of the prepared microbial agent is larger than or equal to 95%, the viable bacterium rate is larger than or equal to 90% after the microbial agent is stored for 12 months at the temperature of 25 DEG C, and the microbial agent shows a remarkable effect in tomato root-knot nematode prevention and treatment of livestock and poultry diarrhea and has good application prospects. The compound can completely or partially replace chemical pesticides and antibiotics, and has a wide market prospect.

Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g / L or at a greater concentration than 1.8 g / L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

The present invention belongs to the technical field of microorganisms, and specifically provides a proliferation medium for improving the heat resistance of Enterococcus faecium, comprising: 1-3% glucose, 1-3% peptone, 0.5-1.5% yeast extract powder, and 0.2-1.5% γ-aminobutyric acid, with the balance being water. The present invention also provides a method for improving the heat resistance of Enterococcus faecium using the above medium and a method for spray-drying to prepare solid Enterococcus faecium powder. The proliferation medium for improving the heat resistance of Enterococcus faecium provided by the present invention can effectively improve the heat resistance of Enterococcus faecium cells themselves, thereby increasing the viable cell retention rate of Enterococcus faecium during high-temperature drying. Under the same production process conditions, the viable cell yield of the product is increased, and the production cost of Enterococcus faecium is greatly reduced.

Provided herein is a composition comprising a hydrogel covalently bound to a functional moiety, wherein the hydrogel comprises a cross linkable biopolymer and a first cell population; the cross linkable biopolymer is at least partially crosslinked by a crosslinking agent within the hydrogel; and the functional moiety has a binding affinity to a cell. Furthermore, a method of growing cells using the composition of the invention, and a method of manufacturing the composition using a kit disclosed herein are also provided.

Application of Lysinibacillus fusiformis in removing hexavalent chromium from eutrophic wastewater, belonging to the field of microbial technology. The strain is preserved in the General Microbiological Center of the China Committee for Culture Collection of Microorganisms, with the preservation address being No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation number being CGMCC No. 10053, and the preservation name being Lysinibacillus fusiformis WTXJ1-4, and the preservation date is November 25, 2014. Through single-factor experiments and orthogonal experiments for optimization, the suitable biological adsorption conditions of this strain for hexavalent chromium in eutrophic wastewater were determined as follows: pH 2.0, initial potassium dichromate concentration 50 mg / L, adsorption time 24 h, wet cell mass dosage 1.0 g / L, adsorption temperature 34 °C, and rotation speed 160 r / min. When reaching equilibrium under these optimized adsorption conditions, the removal rates of hexavalent chromium and total chromium in eutrophic wastewater by viable cells of WTXJ1-4 reached 91.6% - 94.8% and 42.2% - 43.8% respectively. Lysinibacillus fusiformis Viable cells of WTXJ1-4 have strong adsorption-reduction ability, tolerance and resistance to hexavalent chromium in eutrophic wastewater, and their effective ranges are potassium dichromate concentrations of 0 - 100 mg / L, 100 - 300 mg / L, and 300 - 500 mg / L respectively.

Embodiments of the present disclosure provide compositions and methods for injectable recombinant protein-based hydrogels for therapeutic delivery. Embodiments of the composition and methods comprise a recombinant protein, a cell consortia, and a biocompatible medium. The recombinant protein comprises one or more self-association domains and a flexible linker comprising a pseudorepeat of amino acids, forming stable hydrogels in contact with the cell consortia. Such composition and methods have broad application in delivery of viable cells to a biological tissue for tissue repair and regeneration.

This provides a continuous culture process for culturing populations of shear-sensitive cells. [Solution] A continuous culture process for culturing a population of cells consisting of shear-sensitive cells, comprising controlling the gas outflow rate of a bioreactor system so that the gas outflow rate does not exceed 20 m / s, wherein the bioreactor system includes at least 25 L of culture medium, and the population of cells is 20 × 10 6 From cells / mL to 15 × 10 7 The process is characterized by achieving a steady-state viable cell concentration within the range of cells / mL, and the gas efflux rate being controlled at least until the bioreactor system reaches steady-state conditions.

In certain embodiments, this disclosure provides novel methods of increasing the viable cell density of an N−1 large-scale bioreactor cell culture, comprising culturing a host cell expressing a recombinant poly peptide of interest in a non-perfusion-based culture system, and wherein the viable cell density is increased to at least 5×106 cells / mL. In certain embodiments, the disclosure provides novel methods for large-scale production of a recombinant polypeptide of interest, comprising: (1) culturing a host cell expressing a recombinant polypeptide of interest in an N−1 stage in a non-perfusion-based culture system, wherein the viable cell density is increased to at least 5×106 cells / mL; and (2) culturing N fed-batch production cells in an enriched media with high-seed density at least 1.5×106 cells / mL, wherein the N fed-batch production cells are inoculated from the N-1 stage in a non-perfusion-based culture system.