2 results about "Cell size" patented technology

This application relates to the field of biotechnology, specifically disclosing a *Pichia pastoris* yeast, its construction method, and its applications. This application involves knocking out the *gas1* gene, which is related to β-glucan synthesis, in *Pichia pastoris*. By deleting the *gas1* gene, which encodes β-glucan in the cell wall of *Pichia pastoris*, this application reduces cell size, decreases the transfer distance from the cell surface to the nucleus, improves oxygen transfer and product secretion during high-density culture, and enhances fermentation efficiency. Furthermore, the *Pichia pastoris* yeast constructed using this application enhances the synthesis of the intracellular product bisabolol and also enhances its secretion capacity. This application, by reducing cell size and decreasing the carbon flow to cell wall polysaccharide synthesis while increasing the carbon source flow to target product synthesis, has significant implications for industrial production.

The present application relates to a kind of nitrogen-fixing blue-green algae heteromorphosis and fast induction method and application, fast induction is first under the light condition, nitrogen-fixing blue-green algae is placed in nitrogen-deficient culture solution and is induced 12h, then the nitrogen-fixing blue-green algae taken out is placed in nitrogen-deficient phosphorus supply culture solution and continues to be induced 12h;Quantitative is carried out simultaneously to the number of heteromorphosis differentiated, cell size and relative fluorescence intensity using high content cell imaging analysis system;Compared with the induction in nitrogen-deficient culture solution without phosphorus supply, the number of heteromorphosis of three nitrogen-fixing blue-green algae increases by an average of 43.3%, wherein the number of heteromorphosis of anabaena increases by 116%;Nitrogen-deficient phosphorus supply culture mode can accelerate the process of inducing heteromorphosis differentiation, and it is found that under the condition of two-step total induction of 24 hours and 3 times of phosphorus supply, the number of heteromorphosis differentiated by three algae presents the best effect.