12 results about "Cell size" patented technology

The invention discloses a method and a system for estimating plant diversity based on a remote sensing scale effect, which belong to the field of plant diversity monitoring, and are characterized in that a high-resolution remote sensing image of a to-be-monitored area is acquired, and the pixel size is approximately equal to the size of a single plant; extracting a plurality of investigation quadrats in the monitoring area, and obtaining plant diversity true values of the quadrats; preprocessing the remote sensing image to obtain surface reflectance data, and upscaling the high-resolution image by adopting a pixel averaging method to obtain a low-resolution remote sensing image; calculating a nonlinear vegetation index based on the remote sensing images, and calculating a scale deviation of the vegetation index between the remote sensing images with two resolutions; and constructing a mathematical relationship model between the scale deviation and the plant diversity truth value, and applying the mathematical relationship model to the whole remote sensing image so as to estimate the plant diversity of the whole area. According to the method, the plant diversity in a large range can be quickly and accurately estimated, heavy ground survey work is reduced, and manpower, time and economic cost are saved.

The invention relates to the technical field of biology, and particularly discloses pichia pastoris as well as a construction method and application thereof. According to the pichia pastoris disclosed by the invention, beta-glucan is knocked out to synthesize a related gene gas1. By deleting the beta-glucan coding gene gas1 gene of the pichia pastoris cell wall, the cell size is reduced, the transmission distance from the cell surface to the cell nucleus is reduced, oxygen transmission and product secretion during high-density culture are improved, and the fermentation production efficiency is improved. In addition, the pichia pastoris constructed by the invention enhances the synthesis of the intracellular product bisabolol, and also can enhance the secretion ability of the intracellular product bisabolol. By reducing the cell size, reducing the carbon flow flowing to the cell wall polysaccharide synthesis and increasing the carbon source flowing to the target product synthesis, the application has great significance in industrial production.

The invention discloses a method for distinguishing Quercus variabilis and Quercus acutissima, comprising the following steps: firstly, preparing Quercus variabilis sample materials and Quercus acutissima materials, respectively performing vacuuming and fixing treatments on the prepared Quercus variabilis materials and Quercus acutissima materials, obtaining Quercus variabilis solidified samples and Quercus acutissima solidified samples, respectively performing softening and dehydration treatments on the Quercus variabilis solidified samples and Quercus acutissima solidified samples, respectively, obtaining Quercus variabilis dehydrated samples and Quercus acutissima dehydrated samples, obtaining Quercus variabilis transparent samples and Quercus acutissima transparent samples, obtaining Quercus variabilis paraffin samples and Quercus acutissima paraffin samples, obtaining pure Quercus variabilis samples and Quercus acutissima samples, obtaining Quercus variabilis observation samples and Quercus acutissima observation samples, and distinguishing Quercus variabilis and Quercus acutissima by observing the cell size of the samples. By observing the cell morphology of the samples by paraffin section in combination with a microscope, the Quercus variabilis and Quercus acutissima can be intuitively and accurately distinguished, especially for trees of younger age, with high accuracy, and the influence of individual differences can be excluded.

This application relates to the field of biotechnology, specifically disclosing a *Pichia pastoris* yeast, its construction method, and its applications. This application involves knocking out the *gas1* gene, which is related to β-glucan synthesis, in *Pichia pastoris*. By deleting the *gas1* gene, which encodes β-glucan in the cell wall of *Pichia pastoris*, this application reduces cell size, decreases the transfer distance from the cell surface to the nucleus, improves oxygen transfer and product secretion during high-density culture, and enhances fermentation efficiency. Furthermore, the *Pichia pastoris* yeast constructed using this application enhances the synthesis of the intracellular product bisabolol and also enhances its secretion capacity. This application, by reducing cell size and decreasing the carbon flow to cell wall polysaccharide synthesis while increasing the carbon source flow to target product synthesis, has significant implications for industrial production.

The invention belongs to the technical field of bioengineering, and particularly relates to a method for increasing the size of bacteria, recombinant escherichia coli and application of the recombinant escherichia coli. According to the present invention, the microbial cell morphology and the division mode are modified by regulating the microbial cell morphology related protein, such that the characteristics of the microbial cells are optimized so as to improve the application value of the microbial cells in the fields of biological manufacturing and the like. Specifically, escherichia coli with enhanced minE gene or weakened expression of any one or more of mreB, minC, minD, dacA and ftsZ genes is taken as a chassis strain, and the cell size is increased by heterologous expression of DivIVA. According to the invention, large-size cells which can be split, cultured and inherited are obtained, a larger production space is provided for producing some intracellular / extracellular products, the method can be applied to the production of various biomacromolecules, and a new solution is provided for enhancing the production efficiency of target products. Meanwhile, the increased cell morphology is beneficial to the processes of cell interception, adsorption, precipitation, product separation and the like, and the overall production efficiency is improved.

The invention relates to a nitrogen-fixing cyanobacteria heteromorphic cell and a rapid induction method and application thereof, and the rapid induction comprises the following steps: putting nitrogen-fixing cyanobacteria in a nitrogen-deficient culture solution under an illumination condition, inducing for 12 hours, then putting the taken nitrogen-fixing cyanobacteria in a nitrogen-deficient phosphorus-supplemented culture solution, and continuously inducing for 12 hours; meanwhile, a high-content cell imaging analysis system is used for quantifying the quantity, the cell size and the relative fluorescence intensity of the heteromorphic cells formed by differentiation; compared with induction in a culture solution which is deficient in nitrogen and not supplemented with phosphorus, the number of the heteromorphic cells of the three nitrogen-fixing blue-green algae is averagely increased by 43.3%, and the number of the heteromorphic cells of the anabaena is increased by 116%; the culture mode of nitrogen deficiency and phosphorus supply can accelerate the process of inducing heteromorphic cell differentiation, and finds that the number of the heteromorphic cells differentiated from the three strains has the best effect under the conditions of 24 hours of induction in two steps and three times of phosphorus supply.

The invention relates to a method for regulating and controlling the cell division cycle to strengthen the electron transfer capability of shewanella. In order to explore the relationship between the cellular morphology and the electron transfer capability of electroactive microorganisms, a synthetic biology strategy is used for carrying out cellular morphology transformation on mode electrogenic bacteria shewanella, and four engineering strains capable of shortening the cell division cycle of shewanella are constructed. Morphological characterization shows that the cell size of the overexpression division protein engineering strain is reduced, and electrochemical characterization shows that the output voltage, current and power density of the engineering strain are gradually increased along with the reduction of the cell volume, which indicates that the overexpression division related protein can shorten the division cycle and accelerate the cell division process; the cells are reduced; and the electron transfer rate of shewanella is improved. The invention provides a new method for transforming electroactive microorganisms by using morphological engineering, enhancing the electron transfer capability of shewanella and improving the application capability of shewanella.

The present disclosure provides an integrated culture-freeze drying method for Bifidobacterium longum subsp. infantis B8762, which belongs to the field of biotechnology. The method includes: activating a strain in a carbon-source-free modified MRS culture medium and inoculating the activated strain into a fresh culture medium; adding one of D-lactose, D-sucrose or D-maltose; monitoring a growth curve, and building kinetic models in combination with metabolite analysis; collecting bacterial sludge after culture to a stationary phase, preparing a bacterial suspension, and testing a bacterial cell size, the number of growth generations, colony activity and a freeze-drying survival rate; and finally, performing vacuum freeze drying, and monitoring enzyme activity during storage. The method provided by the present disclosure can significantly improve the freeze-drying survival rate, delay attenuation of enzyme activity in a storage phase, and optimize growth and cell activity.

The present application relates to a kind of nitrogen-fixing blue-green algae heteromorphosis and fast induction method and application, fast induction is first under the light condition, nitrogen-fixing blue-green algae is placed in nitrogen-deficient culture solution and is induced 12h, then the nitrogen-fixing blue-green algae taken out is placed in nitrogen-deficient phosphorus supply culture solution and continues to be induced 12h;Quantitative is carried out simultaneously to the number of heteromorphosis differentiated, cell size and relative fluorescence intensity using high content cell imaging analysis system;Compared with the induction in nitrogen-deficient culture solution without phosphorus supply, the number of heteromorphosis of three nitrogen-fixing blue-green algae increases by an average of 43.3%, wherein the number of heteromorphosis of anabaena increases by 116%;Nitrogen-deficient phosphorus supply culture mode can accelerate the process of inducing heteromorphosis differentiation, and it is found that under the condition of two-step total induction of 24 hours and 3 times of phosphorus supply, the number of heteromorphosis differentiated by three algae presents the best effect.

The present disclosure relates to a microchamber device (1) for antibiotic sensitivity testing of bacterial cells (12) of a range of bacterial cell sizes, the microchamber device (1) being arranged on a substrate (40). The invention relates to a microchamber device (1) comprising a microchamber (11), a fluid inlet (14) and a fluid outlet (16) in fluid communication with the microchamber (11), and an integrated sensor (18) arranged in the microchamber (11), the sensor (18) being configured to monitor properties of bacterial cell activity in the microchamber (11) in real time and characterized in that the microchamber (11) comprises at least one trapping device (20), the trapping device (20) being configured to trap bacterial cells in the microchamber (11). And at least one sensor configured to capture at least one bacterial cell (12) in the microchamber (11). The present disclosure also relates to a test system (2) comprising at least one microchamber device (1) and a method for performing an antibiotic sensitivity test on bacterial cells (12).

A polyhydroxyalkanoate-producing microorganism is cultured to obtain microbial bodies accumulating polyhydroxyalkanoate particles and having an average cell size of 2 μm or more. The microbial bodies are subjected to a heat treatment at a temperature higher than a temperature in the culturing to increase an average particle size of the polyhydroxyalkanoate particles in the microbial bodies. The resulting average particle size is equal to or greater than 1.8 μm and equal to or smaller than the average cell size. The microbial bodies subjected to the heat treatment can be disrupted to obtain a cell disruption solution. The PHA particles can be separated from an aqueous phase of the cell disruption solution.

A method of cultivating algal cells of an algae belonging to a class selected from Chlorophyceae, Euglenophyceae, Bacillariophyceae and Haptophyceae includes: irradiating the algal cells with an artificial light having a ratio of (i) photon flux density in a wavelength range of 520-630 nm to (ii) photosynthetic photon flux density, that is 65% or more; and measuring a cell size of the algal cells. Irradiation and non-irradiation of the algal cells with the artificial light are switched, or the photon flux density in the wavelength range of 520-630 nm is changed, according to the measured cell size.