4 results about "Cell size" patented technology

The invention relates to the technical field of biology, and particularly discloses pichia pastoris as well as a construction method and application thereof. According to the pichia pastoris disclosed by the invention, beta-glucan is knocked out to synthesize a related gene gas1. By deleting the beta-glucan coding gene gas1 gene of the pichia pastoris cell wall, the cell size is reduced, the transmission distance from the cell surface to the cell nucleus is reduced, oxygen transmission and product secretion during high-density culture are improved, and the fermentation production efficiency is improved. In addition, the pichia pastoris constructed by the invention enhances the synthesis of the intracellular product bisabolol, and also can enhance the secretion ability of the intracellular product bisabolol. By reducing the cell size, reducing the carbon flow flowing to the cell wall polysaccharide synthesis and increasing the carbon source flowing to the target product synthesis, the application has great significance in industrial production.

This application relates to the field of biotechnology, specifically disclosing a *Pichia pastoris* yeast, its construction method, and its applications. This application involves knocking out the *gas1* gene, which is related to β-glucan synthesis, in *Pichia pastoris*. By deleting the *gas1* gene, which encodes β-glucan in the cell wall of *Pichia pastoris*, this application reduces cell size, decreases the transfer distance from the cell surface to the nucleus, improves oxygen transfer and product secretion during high-density culture, and enhances fermentation efficiency. Furthermore, the *Pichia pastoris* yeast constructed using this application enhances the synthesis of the intracellular product bisabolol and also enhances its secretion capacity. This application, by reducing cell size and decreasing the carbon flow to cell wall polysaccharide synthesis while increasing the carbon source flow to target product synthesis, has significant implications for industrial production.

The present disclosure provides an integrated culture-freeze drying method for Bifidobacterium longum subsp. infantis B8762, which belongs to the field of biotechnology. The method includes: activating a strain in a carbon-source-free modified MRS culture medium and inoculating the activated strain into a fresh culture medium; adding one of D-lactose, D-sucrose or D-maltose; monitoring a growth curve, and building kinetic models in combination with metabolite analysis; collecting bacterial sludge after culture to a stationary phase, preparing a bacterial suspension, and testing a bacterial cell size, the number of growth generations, colony activity and a freeze-drying survival rate; and finally, performing vacuum freeze drying, and monitoring enzyme activity during storage. The method provided by the present disclosure can significantly improve the freeze-drying survival rate, delay attenuation of enzyme activity in a storage phase, and optimize growth and cell activity.

The present application relates to a kind of nitrogen-fixing blue-green algae heteromorphosis and fast induction method and application, fast induction is first under the light condition, nitrogen-fixing blue-green algae is placed in nitrogen-deficient culture solution and is induced 12h, then the nitrogen-fixing blue-green algae taken out is placed in nitrogen-deficient phosphorus supply culture solution and continues to be induced 12h;Quantitative is carried out simultaneously to the number of heteromorphosis differentiated, cell size and relative fluorescence intensity using high content cell imaging analysis system;Compared with the induction in nitrogen-deficient culture solution without phosphorus supply, the number of heteromorphosis of three nitrogen-fixing blue-green algae increases by an average of 43.3%, wherein the number of heteromorphosis of anabaena increases by 116%;Nitrogen-deficient phosphorus supply culture mode can accelerate the process of inducing heteromorphosis differentiation, and it is found that under the condition of two-step total induction of 24 hours and 3 times of phosphorus supply, the number of heteromorphosis differentiated by three algae presents the best effect.