138 results about "Anabaena" patented technology

The present invention discloses a high protein growth promoting disease-resistant feed for freshwater shrimps. The feed consists of the following raw materials in parts by weight: 25-45 parts of fish meal, 5-15 parts of wheat bran, 2-8 parts of squid paste, 3-9 parts of squid powder, 1-5 parts of soybean meal, 2-6 parts of rice bran, 1-4 parts of distiller's grains, 3-6 parts of tenebrio molitor powder, 1-5 parts of tenebrio molitor skins, 2-6 parts of anabaena powder, 1-4 parts of corn gluten meal, 3-6 parts of crab shell powder, 1-5 parts of shrimp shell powder, 1.5-4.5 parts of soybean phospholipids, 3-5 parts of palm oil, 2-6 parts of fish oil, 1-4 parts of yeast powder, 3-5 parts of calcium dihydrogenphosphate, 1-4 parts of calcium lactate, 2-5 parts of Chinese herbal medicine additives, 1-3 parts of vitamin premixes, 1-4 parts of amino acid premixes, 3-9 parts of mineral premixes, and 1-5 parts of an adhesion agent. The feed for the freshwater shrimps is rich in proteins, can promote the growth of the freshwater shrimps, improve the disease resistance of the freshwater shrimps, and thus can effectively improve the yield and quality of the freshwater shrimps.

The invention relates to a specific compound microbial deodorizer. The specific compound microbial deodorizer contains a microbial agent, a plant extract, an alga extract and water, wherein the microbial agent contains 20%-40% of lactobacillus plantarum and 20%-40% of beer yeast; the alga extract is 0.5%-2% of a nostoc wall-broken extract or 0.5%-2% of an anabaena wall-broken extract or 0.5%-2% of a spirulina wall-broken extract. The specific compound microbial deodorizer is low in cost, high in efficiency and wide in application range and is very practical in specific production.

The invention relates to a resourceful treatment method for waste bodies of dead livestock and poultry animals. The resourceful treatment method comprises the following steps: refrigerating, storing and cutting dead animals, and crushing the refrigerated dead animals to obtain animal fragments; cooking the animal fragments; mixing the oil-removed animal fragments with a compound microbial agent to carry out micro-biological degradation; and carrying out medium-temperature aerobic fermentation on a degraded product to obtain biogas, biogas slurry and biogas residues, wherein the compound microbial agent consists of anabaena, bacillus subtilis, lactobacillus acidophilus, bacillus megatherium, bacillus cereus, bacillus pumilus, slender rhodospirillum, stenotrophomonas maltophilia and bacillus mojavensis. The resourceful treatment method solves the problem of treating dead bodies of the livestock and poultry animals of a large-scale breeding plant, so that resources are reused.

The invention provides an immobilized algae-lysing microorganism as well as a preparation method and an application thereof. The immobilized algae-lysing microorganism is prepared from raw materials in percentage by mass as follows: 40%-60% of algae-lysing microorganisms, 30%-50% of a carrier and 10%-20% of an immobilization material, wherein the algae-lysing microorganisms are one or more of bacillus, streptomyces, pseudomonas, sphingomonas, mycobacterium and shewanella with algae-lysing activity and are liquid, powdery or granular substances, with the viable count not lower than 1.0*10<10> cfu / g. The immobilized algae-lysing microorganism has a large specific surface area, good adsorption performance, high algae-lysing efficiency and environment adaptability, no secondary risk and the like, particularly has remarkable algae-lysing effects on microcystis, anabaena, aphanizomenon and other typical harmful algae and has better application prospects in the field of bioremediation of eutrophic water.

The invention discloses a method for improving acidified soil and enhancing soil fertility by the aid of nitrogen-fixation cyanobacteria. The method includes steps of 1), selecting anabaena to be usedas nitrogen-fixation cyanobacteria application algae species, and carrying out step-by-step extended culture; 2), deeply plowing crop rotation fields by the depths of 15-20 cm at early-season rice transplanting earlier stages in double-cropping rice modes at first, transplanting rice, then applying nitrogen, phosphorus and potassium fertilizers, inoculating the nitrogen-fixation cyanobacteria according to a quantity of 3-5 kg of fresh alga for each mu, harvesting early-season rice, then shallowly plowing the crop rotation fields by the shallow plowing depths of 5-10 cm, planting late rice, and applying the nitrogen-fixation cyanobacteria and nitrogen, phosphorus and potassium fertilizers according to the same process; alternately, deeply plowing the crop rotation fields by the depths of 15-20 cm at first at rice transplanting earlier stages in single-cropping rice modes, transplanting the rice, then applying the nitrogen, phosphorus and potassium fertilizers, and inoculating the nitrogen-fixation cyanobacteria according to a quantity of 5-7 kg of fresh alga for each mu. The method has the advantages that the application amount of nitrogen fertilizers can be reduced by 50%, the nitrogen-fixation cyanobacteria can be fed into red soil along with deep plowing or shallow plowing, the organic matter content of the red soil can be increased after the nitrogen-fixation cyanobacteriais decomposed, and accordingly the soil fertility level of the red soil can be comprehensively enhanced.

The invention belongs to the food industry biotechnology field, and relates to a method for high-efficiency production of recombinant lipoxygenase by using Bacillus subtilis. The method comprises the following steps: (1) constructing Anabaena lipoxygenase recombinant Bacillus subtilis genetic engineering bacteria; (2) inoculating the Anabaena lipoxygenase recombinant Bacillus subtilis genetic engineering bacteria in a LB medium which possesses liquid volume of 58-63mL / 250mL and is filled with 15-18g / L lactose, cultivating at the conditions of 27-30 DEG C and 180rpm for 85-90h, centrifuging and collecting a supernatant. Under the best conditions, activity of the recombinant lipoxygenase can reach 167.32U / mL, and the method of the invention provides certain guidance for large-scale production of the recombinant lipoxygenase.

The invention relates to an induction method of anabaena flosaquae chlamydospore in the field of biotechnologies. The induction method comprises the following steps of: culturing the anabaena flosaquae for 3-4 days under the conditions of adding soil extract, cane sugar or glucose to a liquid culture medium containing dipotassium phosphate, ferric citrate, molybdic acid and calcium carbonate and regulating pH to be 6-7 to realize induction of the chlamydospore; and laying the anabaena flosaquae on a porcelain disc or a big culture vessel of 150*15mm, placing the porcelain disc or the big culture vessel into an oven and baking or placing the porcelain disc or the big culture vessel into a disinfection chamber of 30 DEG C and drying for 3-5 days to obtain dry algae powder. In the invention,the chlamydospore content is greatly improved because of effective and reasonable conditions, the dry algae powder can be obtained more efficiently through a simple drying mode, the method for preparing the dry algae powder with bloom cyanobacteria as a raw material is simple in process, the application of fertilizer is safe and efficient, the method is easy for popularization and implementation,and after the fertilizer is utilized, new pollution on environment and food safety is not brought about, therefore, the induction method of the anabaena flosaquae chlamydospore has great theoretical value and application value.

The invention discloses a mutant of phenylalanine deaminase with an anabaena source and belongs to protein engineering. The phenylalanine deaminase E75L obtained in the invention has trypsin degradation resistance and low pH resistance which are greatly improved compared with those of wild-type enzymes. The phenylalanine deaminase E75L provided by the invention achieves the effect of promoting application of the phenylalanine deaminase in oral medication of phenylketonuria (PKU) to a certain degree.

The invention belongs to the technical field of cosmetic compositions, in particular to a long-acting moisturizing composition, a preparation method and an application thereof. The moisturizing composition comprises a fermented fucoidan extract, astragalus extract and Anabaena pseudoanabaena extract as active ingredients. 11) adding water into fucoidan powder, astragalus powder and Anabaena pseudoanabaena powder for extracting to obtain extract; (2) adding a fermentation component containing Saccharomyces cerevisiae into the extract in the step (1) to prepare a fermentation broth; (3) autoclaving the fermentation broth, inoculating Bacillus licheniformis and Aspergillus oryzae after cooling to produce the active ingredient. The moisturizing cream prepared by the composition of the invention can achieve a long-term moisturizing effect of 12 hours, can quickly spread and absorb, and the skin content can be maintained at a high value for 12 hours. Moreover, the relative irritation is small, can quickly spread and absorb, skin feel refreshing.

The invention relates to a synthetic oligopeptide containing three aminophenols; cysteine is in the middle of a sequence, one end of the sequence is glutamic acid or glycin, and the other end of the sequence is aminophenol proline or Gamma-propalanine having antiretroviral function. The function of the synthetic oligopeptide is similar to that of glutathione, i.e., removing 1, 1-DPPH free radicals under in-vitro condition, improving the oxidation resistance of anabaena and tobacco cells, and the activity of oxidizing enzymes under salt stress, and improving the heat resistance of wheat seedlings.

The invention discloses bacillus licheniformis and an application thereof. The bacillus licheniformis is named as bacillus licheniformis BWFA55, the preservation unit is Guangdong Province Microbial Culture Collection Center (GDMCC), the address is 5th floor, building 59, courtyard 100, Xianlie Middle Road, Guangzhou, the preservation date is December 8th, 2020, and the preservation number is GDMCC No: 61354. The bacillus licheniformis is applied to killing one or more harmful algae including anabaena flosaquae, microcystis aeruginosa and aphanizomenon flosaquae. The bacillus licheniformis isused for preventing water bloom and red tide. The bacillus licheniformis is applied to water environment treatment in aquaculture.

The invention discloses an efficient, compound and organic fertilizer for garden plants and flowers and a preparation method thereof. The efficient, compound and organic fertilizer is prepared from, by weight, 11-22 parts of industrial vinasse materials, 6-11 parts of wormcast, 8-15 parts of magnetized water straw, 13-20 parts of unsaturated polyester resin powder, 4-8 parts of sodium humate and anabaena, 2.5-6 parts of aspergillus oryzae powder, 15-21 parts of compound amino acid liquid and 7-12 parts of organic fertilizer. The green fertilizer is simple in formula, the raw materials are natural and rich, are environmentally friendly and are easy to obtain, resource recycling is achieved by utilizing the magnetized water straw, the production cost is reduced, the green fertilizer stable in performance and higher in quality is obtained, the fertilizer efficiency is good, the fertility of soil is improved by a soil activating agent, the soil is improved, the soil tilth is improved, and a material guarantee is provided for fertile soil projects. The properties of soil can be effectively improved, and the fertility of soil is improved. In addition, the raw materials for the method are cheap and easy to obtain, the process is simple, and the method is easy to popularize.

The invention discloses a grass carp bait, consisting of salvia miltiorrhiza, chinese azalea, tobacco leaf, ginger, nux vomica, cinnamomum camphorapresi, Shuizhongxiang, anabaena, grass carp powder hunger promoter, corn swelling powder, paddy, and fresh earthworn. The first six materials are spread out in the sun to dry, mixed in proportion, then ground to powder and put into a container to seal. The earthworn is cut into 2cm sections for use. The paddy is put into the pot, water is added to boil with the amount of no water drop if the cooked paddy is pinched by the hand, then the cooked paddy is scattered immediately in hot, and the mix powder of salvia miltiorrhiza, chinese azalea, tobacco leaf, ginger, nux vomica, cinnamomum camphorapresi is poured on the cooked paddy, mixed evenly and quickly to achieve the adherence of dry powder on the cooked paddy, covered, and sealed for 3 to 4 hour, then the Shuizhongxiang, anabaena, grass carp powder hunger promoter, corn swelling powder, and fresh earthworn are all poured into the pot and mixed evenly, and put into a big plastic bag for sealing.

The invention belongs to the technical field of water pollution control and particularly relates to a method for purifying water by killing algae by using micro-current and the application of the method. The method is characterized in that two electrodes are put in water with algae and connected with a direct-current motor so that the upper electrode and the lower electrode have a certain current density, and the alga cells are killed after a certain period of time and sunken to the bottom of the water so that the water is purified. The micro-current has the significant function of killing a variety of algae including microcystis, aphanizomenon, anabaena, quadrigula chodatii and scenedesmus and is suitable for lakes, ponds and other water environment.

The invention relates to a 100-liter close type photo-bioreactor and a processing technique for the mass cultivation and promotion of the growth and the gene expression of trans-TNF-Alfa anabaena by using the photo-bioreactor. The reactor comprises three built-in light transmitting glass tube, an adjustable illumination facility, a sterile air blowing facility, a high temperature sterilizing system, and CO2 distribution facility. A culture medium is prepared, and regulated and controlled artificially in the reactor so as to promote the growth and the gene expression of the trans-TNF-Alfa anabaena. The production method is suitable for large scale production of transgene blue algae, and has the advantages of high stability, strong controllability and maneuverability, small outcome difference between batches, etc.

The invention provides a laying hen feed containing a Chinese herbal medicine additive, and relates to the technical field of feeds. The feed consists of a basal feed and a Chinese herbal medicine additive, wherein the basal feed comprises corn, soybean meal, bran, alfalfa meal, pumpkin powder, stone powder, calcium hydrophosphate, edible salt and a premix; wherein the Chinese herbal medicine additive comprises ligusticum wallichii, Chinese wolfberry, motherwort, liquorice, angelica sinensis, anabaena sinensis, euphorbia lathyris and rice hull powder. The feed disclosed by the invention meetsthe requirements of laying hens on nutrients such as protein, starch, calcium, phosphorus, trace mineral elements and vitamins; the palatability and yolk color of the feed are improved, intestinal peristalsis and intestinal health of the laying hens are promoted, the body immunity of the laying hens is improved, various poultry diseases are prevented and treated, the egg laying rate is increased,the fishy smell of eggs is favorably removed, and the egg quality is improved.

The invention discloses pyrolysis phycophage MACPNOA1 and a separation method and application thereof, and relates to biological treatment of eutrophic water. MACPNOA1 is preserved in China General Microbiological Culture Collection Center in October 24th, 2016, and the preservation number is CGMCC No.12996. MACPNOA1 has a wide alge-lysing spectrum, and widely lyses common water-blooming cyanobacteria; when MACPNOA1 is added into cyanobacterial water-blooming liquid belonging to seven categories of Microcystis, Aphanizomenon, Chroococcus, Planktothrix, Nostoc, Oscillatoria and Anabaena, MACPNOA1 has strong pyrolysis effects on all the measured seven categories. When MACPNOA1 is applied to algal bloom treatment, the obvious effect of protecting animals in water is achieved. The pyrolysis phycophage MACPNOA1 has the advantages of being safe, efficient and quick.

The invention discloses organic nitrogenous fertilizer and a preparation method thereof. The organic nitrogenous fertilizer is prepared from, by weight, 22-24 parts of alfalfa nodules, 18-20 parts of sweet clover, 20-22 parts of red clover nodules, 8-10 parts of scirpus tabernaemontani, 10-12 parts of ant eggs, 15-17 parts of frog eggs, 16-18 parts of earthworm cocoons, 25-27 parts of urine, 10-12 parts of salvinia natans, 17-19 parts of chlorella, 16-18 parts of anabaena, 1-3 parts of EM stock solutions, 2-4 parts of sucrose, 20-22 parts of grass carbon and 18-20 parts of tea seed cakes. The organic nitrogenous fertilizer has a large amount of nitrogen, and is strong in pertinence and capable of promoting plant growth and improving agricultural product yield and quality.

The invention relates to a medicine for treating chronic sinusitis which is prepared by mixing leech, earthworm and plantain seed that are processed, flos magnoliae liliflorae, caulis akebiae, spica schizonepetae, honeysuckle, forsythia, radix scutellariae, golden cypress, anabaena, salvia miltiorrhiza, raw astragalus root, rehmanniae praeparatum, the root bark of the peony tree, ligusticum sinense oliver, bitter orange and liquorice; adding water into the mixture and decocting; merging decocted liquid; filtering and concentrating decocted liquid into clear paste, drying and grinding the clearpaste, and putting into capsules. The medicine has the effects of clearing away heat and toxic material, replenishing qi and invigorating the spleen, dispelling wind and eliminating dampness, and dissipating phlegm and promoting the restoration consciousness, so as to be used for treating chronic sinusitis with the total effective rate of 95.0%. The medicine also has the advantages of treating the symptoms and the root causes, high cure rate and no toxic side effect.

The invention relates to a microbial deodorant containing Bifidobacterium Anabaena. The deodorant is prepared from a microbial agent, a plant extract, an alga extract, a bioactive enzyme and water; the microbial agent comprises 15%-35% of Lactobacillus plantarum, 15%-35% of saccharomyces cerevisiae, 2%-6% of Bifidobacterium, 2%-6% of nitrifying bacteria and 3%-5% of bacillus; the plant extract comprises 4%-15% of a heartleaf houttuynia herb extract and 3%-8% of a mint extract; the alga extract comprises 0.5%-2.5% of a brown alga broken-wall extract; and the percentage is the mass percentage. The deodorant is low in cost, high in efficiency and wide in application range and is very practical in specific production, and the garbage disposal efficiency is greatly improved.

The invention discloses compound organic fertilizer and a preparation method thereof. The compound organic fertilizer is prepared from, by weight, 15-20 parts of sodium bicarbonate, 10-15 parts of potassium alum, 8-10 parts of calcium carbonate, 4-6 parts of vanillin, 12-14 parts of halloysite, 6-8 parts of limestone, 18-20 parts of steamed rice dumpling leaves, 22-25 parts of creeping oxalis, 25-30 parts of coconut shells, 35-40 parts of soybean hulls, 18-20 parts of furfural residues, 12-15 parts of soy sauce residues, 10-12 parts of grape peel, 7-9 parts of sodium humate and 12-15 parts of anabaena. The compound organic fertilizer is durable in fertilizer efficiency, long in fermentation time, free of peculiar smell and low in manufacturing cost due to the adopted raw materials.

The invention discloses a microalgae nutrition repair liquid for relieving grassland desertification, which comprises a microalgae mixed liquid, the microalgae mixed liquid comprises seven active microalgae, and the added microalgae comprises the following components in parts by weight: 5-40 parts of hylothrix tadeeri, 5-40 parts of hylothrix wonderi, 10-50 parts of microcoleus vaginalis, 5-40 parts of pseudocladoidea, 10-50 parts of monofidus microfidus and 30-80 parts of chlorella pyrenoidosa, and the microalgae mixed liquid comprises the following components in parts by weight: 5-40 parts of hylothrix tadeeri, 5-40 parts of hylothrix wonderi, 10-50 parts of microcoleus vaginalis and 30-80 parts of chlorella pyrenoidosa. In addition, 10-50 parts of nitrogen-fixing anabaena or 20 parts of cotton algae are selected to be added. Aiming at the grassland desertification region, the nutrient restoration liquid can restore soil, supplement natural nitrogen, activate soil trace elements, improve plant stress resistance and prevent plant diseases and insect pests, the seven microalgae have good compatibility, the number of microalgae active cells in each 1mL of the nutrient restoration liquid can reach more than 106, and the nutrient restoration liquid has good application prospects. The storage life of the nutrient repair liquid reaches 18 months, the culture process is obtained through a large number of experiments, and the culture efficiency is high.

The present invention provides a method for detecting the presence of microcystin-producing toxic cyanobacteria in a sample comprising the step of contacting nucleic acid obtained from an environmental sample with primers specifically hybridizing with the nucleic acid sequence of mcy B gene present in Microcystis aeruginosa, Anabaena sp. and Planktothrix agardhii. The present invention further provides primers, primer pairs and probes designed for the method of the invention.

The invention discloses a photosynthetic type microbial preparation for aquaculture. The photosynthetic type microbial preparation for aquaculture is prepared from the following components in parts by weight: 8-10 parts of chroococcoid, 6-8 parts of oscillatoria, 6-8 parts of nostoc, 5-7 parts of anabaena, 4-5 parts of spiral seaweeds, 1-2 parts of rhodospirillum, 1-2 parts of rhodop seudanonas palustris and 1-2 parts of red micro bacteria. In this way, the photosynthetic type microbial preparation for aquaculture has the advantages of being complete in disinfection, cutting off viral infection, purifying water quality, achieving harmless standards, being natural and free of toxicity, increasing an aquaculture survival rate, and the like, and has a wide market prospect on popularization of the photosynthetic type microbial preparation for aquaculture.

The invention relates to anabaena. The anabaena is preserved in CCTCC (China Center for Type Culture Collection) with preservation number being CCTCC NO:M 2017644 on 31st, October, 2017. The inventionfurther relates to an application of the anabaena in removal of Cd in the environment and a method for removing Cd in the environment. The anabaena can efficiently adsorb Cd<2+> in a water body, so that the anabaena can be used for treating the water body with high Cd<2+> content to remove Cd<2+> in the water body and reduce Cd pollution; the anabaena particularly can be used for controlling Cd pollution of rice irrigation water, and accordingly, Cd content of rice is reduced.

The invention discloses application of nitrogen-fixing anabaena to promote growth of mulberry trees and increase yield of mulberry leaves. The application comprises: collecting nitrogen-fixing anabaena, adjusting water content of the nitrogen-fixing anabaena to less than 15%, and pulverizing; and performing digestion on the pulverized nitrogen-fixing anabaena in ethanol to obtain an extract liquidand residues respectively; concentrating and drying the extract liquid, to obtain dry powders, dissolving the dry powders in water to obtain a spray liquid, and spraying the spray liquid on mulberrybranches and leaves; applying the residues in mulberry root soil. The nitrogen-fixing anabaena is creatively and simply treated to extract the extract liquid and the residues, and the extract liquid is concentrated and made into dry powders, the dry powders are made into the spray liquid to spray on the mulberry branches and leaves, and the residues are applied to the mulberry root soil. Yield ofmulberry leaves in each harvesting period is significantly increased during a growth period of mulberry trees, thereby significantly promoting the growth of mulberry trees.

The invention provides a method for extracting streptomyces alga dissolution active substances, and belongs to the field of eutrophic water body bioremediation. An isometric absolute ethyl alcohol isused for repeatedly extracting streptomyces fermentation liquor filtrate, after centrifugalization is completed, a rotary evaporator is used for concentrating an organic phase to be dry, crude substances are obtained, then methyl alcohol is used for dissolving the crude substances, and silica gel column chromatography separation is carried out; the components with efficient alga dissolution activity are subjected to high-performance liquid chromatographic separation, and the alga dissolution active substances are obtained. Adopted streptomyces comes from an eutrophic water body, the alga dissolution active substances secreted by the streptomyces are secondary metabolites, high safety is achieved, and no secondary pollution is caused to the water body. The alga dissolution active substancescan be used for treatment of the eutrophic water body caused by typical harmful algae such as microcystis, anabaena, aphanizomenon and oscillatoria. The extraction method is simple in process, high in operability, low in production cost, good in alga dissolution effect and suitable for large-scale production.

The invention relates to a composite microbe deodorant having enterococcus faecalis, pine needle, mint and anabaena. The composite microbe deodorant is composed of a microbial agent, a plant extract product, an algae extract product and water. The microbe inocula comprises the following components: 20-40% of plant lactobacillus, 20-40% of brewers yeast, 1-5% of improved denitrifying bacteria, 1-5% of improved nitrobacteria, and 5-10% of enterococcus faecalis; the plant extract product comprises 5-10% of a pine needle product, 5-10% of a radix codonopsis extract product and 2-5% of mint essential oil; and the algae extract product is 0.5-2% of an anabaena wall-breaking extract product, wherein the percentage is mass percentage. The composite microbe deodorant of the invention has the advantages of low cost, high efficiency, and wide adaptation, and is very useful in concrete production.

The invention discloses a medicine for treating acute and chronic rhinitis and sinusitis, in particular a Chinese patent medicine prepared from Chinese herbal medicines. The medicine of the present invention is mainly composed of magnolia, cocklebur, angelica, fangfeng, licorice, notopterygium, asarum, chuanxiong, chrysanthemum, chrysanthemum, scutellaria baicalensis, forsythia, isatidis, fishy Grass root, madder, bletilla striata, or Tongcao, Passepartout or acupuncture point Yanyingxiang, or add astragalus, codonopsis, the present invention has good curative effect on treating acute and chronic rhinitis and sinusitis, low cost and no side effects.