138 results about "Anabaena" patented technology

This invention relates to new use of marine cyanobacteria lyngbya, Oscillatoria, Sprulina, Anabaena and Synechocystis deposited with ATCC having accession numbers ATCC PTA-4602 and 4603 for the removal of calcium ions from sea-brine and sub-soil brine having a density range 10 to 25.5° Be', by culturing the cyanobacteria, inoculating the cyanobacteria culture to raw brine of 10 to 25.5° Be', filtering the resultant mixture to obtain a brine having less calcium, and to separate the cyanobacteria which can be reused if desired.

The invention discloses a method for improving acidified soil and enhancing soil fertility by the aid of nitrogen-fixation cyanobacteria. The method includes steps of 1), selecting anabaena to be usedas nitrogen-fixation cyanobacteria application algae species, and carrying out step-by-step extended culture; 2), deeply plowing crop rotation fields by the depths of 15-20 cm at early-season rice transplanting earlier stages in double-cropping rice modes at first, transplanting rice, then applying nitrogen, phosphorus and potassium fertilizers, inoculating the nitrogen-fixation cyanobacteria according to a quantity of 3-5 kg of fresh alga for each mu, harvesting early-season rice, then shallowly plowing the crop rotation fields by the shallow plowing depths of 5-10 cm, planting late rice, and applying the nitrogen-fixation cyanobacteria and nitrogen, phosphorus and potassium fertilizers according to the same process; alternately, deeply plowing the crop rotation fields by the depths of 15-20 cm at first at rice transplanting earlier stages in single-cropping rice modes, transplanting the rice, then applying the nitrogen, phosphorus and potassium fertilizers, and inoculating the nitrogen-fixation cyanobacteria according to a quantity of 5-7 kg of fresh alga for each mu. The method has the advantages that the application amount of nitrogen fertilizers can be reduced by 50%, the nitrogen-fixation cyanobacteria can be fed into red soil along with deep plowing or shallow plowing, the organic matter content of the red soil can be increased after the nitrogen-fixation cyanobacteriais decomposed, and accordingly the soil fertility level of the red soil can be comprehensively enhanced.

The invention belongs to the food industry biotechnology field, and relates to a method for high-efficiency production of recombinant lipoxygenase by using Bacillus subtilis. The method comprises the following steps: (1) constructing Anabaena lipoxygenase recombinant Bacillus subtilis genetic engineering bacteria; (2) inoculating the Anabaena lipoxygenase recombinant Bacillus subtilis genetic engineering bacteria in a LB medium which possesses liquid volume of 58-63mL / 250mL and is filled with 15-18g / L lactose, cultivating at the conditions of 27-30 DEG C and 180rpm for 85-90h, centrifuging and collecting a supernatant. Under the best conditions, activity of the recombinant lipoxygenase can reach 167.32U / mL, and the method of the invention provides certain guidance for large-scale production of the recombinant lipoxygenase.

The invention relates to a 100-liter close type photo-bioreactor and a processing technique for the mass cultivation and promotion of the growth and the gene expression of trans-TNF-Alfa anabaena by using the photo-bioreactor. The reactor comprises three built-in light transmitting glass tube, an adjustable illumination facility, a sterile air blowing facility, a high temperature sterilizing system, and CO2 distribution facility. A culture medium is prepared, and regulated and controlled artificially in the reactor so as to promote the growth and the gene expression of the trans-TNF-Alfa anabaena. The production method is suitable for large scale production of transgene blue algae, and has the advantages of high stability, strong controllability and maneuverability, small outcome difference between batches, etc.

The invention provides a laying hen feed containing a Chinese herbal medicine additive, and relates to the technical field of feeds. The feed consists of a basal feed and a Chinese herbal medicine additive, wherein the basal feed comprises corn, soybean meal, bran, alfalfa meal, pumpkin powder, stone powder, calcium hydrophosphate, edible salt and a premix; wherein the Chinese herbal medicine additive comprises ligusticum wallichii, Chinese wolfberry, motherwort, liquorice, angelica sinensis, anabaena sinensis, euphorbia lathyris and rice hull powder. The feed disclosed by the invention meetsthe requirements of laying hens on nutrients such as protein, starch, calcium, phosphorus, trace mineral elements and vitamins; the palatability and yolk color of the feed are improved, intestinal peristalsis and intestinal health of the laying hens are promoted, the body immunity of the laying hens is improved, various poultry diseases are prevented and treated, the egg laying rate is increased,the fishy smell of eggs is favorably removed, and the egg quality is improved.

The invention discloses organic nitrogenous fertilizer and a preparation method thereof. The organic nitrogenous fertilizer is prepared from, by weight, 22-24 parts of alfalfa nodules, 18-20 parts of sweet clover, 20-22 parts of red clover nodules, 8-10 parts of scirpus tabernaemontani, 10-12 parts of ant eggs, 15-17 parts of frog eggs, 16-18 parts of earthworm cocoons, 25-27 parts of urine, 10-12 parts of salvinia natans, 17-19 parts of chlorella, 16-18 parts of anabaena, 1-3 parts of EM stock solutions, 2-4 parts of sucrose, 20-22 parts of grass carbon and 18-20 parts of tea seed cakes. The organic nitrogenous fertilizer has a large amount of nitrogen, and is strong in pertinence and capable of promoting plant growth and improving agricultural product yield and quality.

The invention discloses a microalgae nutrition repair liquid for relieving grassland desertification, which comprises a microalgae mixed liquid, the microalgae mixed liquid comprises seven active microalgae, and the added microalgae comprises the following components in parts by weight: 5-40 parts of hylothrix tadeeri, 5-40 parts of hylothrix wonderi, 10-50 parts of microcoleus vaginalis, 5-40 parts of pseudocladoidea, 10-50 parts of monofidus microfidus and 30-80 parts of chlorella pyrenoidosa, and the microalgae mixed liquid comprises the following components in parts by weight: 5-40 parts of hylothrix tadeeri, 5-40 parts of hylothrix wonderi, 10-50 parts of microcoleus vaginalis and 30-80 parts of chlorella pyrenoidosa. In addition, 10-50 parts of nitrogen-fixing anabaena or 20 parts of cotton algae are selected to be added. Aiming at the grassland desertification region, the nutrient restoration liquid can restore soil, supplement natural nitrogen, activate soil trace elements, improve plant stress resistance and prevent plant diseases and insect pests, the seven microalgae have good compatibility, the number of microalgae active cells in each 1mL of the nutrient restoration liquid can reach more than 106, and the nutrient restoration liquid has good application prospects. The storage life of the nutrient repair liquid reaches 18 months, the culture process is obtained through a large number of experiments, and the culture efficiency is high.

The invention relates to anabaena. The anabaena is preserved in CCTCC (China Center for Type Culture Collection) with preservation number being CCTCC NO:M 2017644 on 31st, October, 2017. The inventionfurther relates to an application of the anabaena in removal of Cd in the environment and a method for removing Cd in the environment. The anabaena can efficiently adsorb Cd<2+> in a water body, so that the anabaena can be used for treating the water body with high Cd<2+> content to remove Cd<2+> in the water body and reduce Cd pollution; the anabaena particularly can be used for controlling Cd pollution of rice irrigation water, and accordingly, Cd content of rice is reduced.