7 results about "Anabaena" patented technology

The invention discloses a sequence optimization method for improving the stability of circular RNA, application and a product, and belongs to the technical field of biotechnology and molecular biology. In order to solve the problem that the existing circular RNA is easy to be specifically degraded by RNAseK and lysosome after entering a cell, synonymous mutation optimization is carried out on an open reading frame of a target gene of the circular RNA by identifying nucleic acid motifs (a first type of nucleic acid motifs and a second type of nucleic acid motifs) identified by two degradation mechanisms. The optimized circular RNA is prepared by means of in-vitro cyclization of an anabaena I-type intron self-splicing mediator, after cells are transfected, the intracellular RNA expression level of the circular RNA is remarkably improved compared with that of a wild type, and the target protein expression efficiency is also remarkably improved. The intracellular stability and expression efficiency of the circular RNA are remarkably enhanced, and the method can be widely applied to the scenes of gene expression regulation, protein synthesis, gene therapy, vaccine development and the like.

The invention provides a soil improvement and fertilization method for salinized moisture soil, and belongs to the technical field of soil fertilizers and fertilization methods. The method comprises the following steps: applying multi-source carbon including first agricultural wastes, decomposed manure and modified carbon materials into salinized moisture soil before sowing, and applying microalgae compounds (45-55% of chlorella, 20-30% of anabaena and 20-30% of micromonofidus) during crop growth to complete improvement of the salinized moisture soil. The invention provides a biophysical comprehensive improvement method giving consideration to soil fertility improvement (organic carbon accumulation improvement) and barrier elimination (salt / sodium modification, structural hardening, permeability and other limiting factors improvement), efficient colonization and continuous activity of microalgae are promoted, and the carbon sequestration and fertility improvement and salt reduction and barrier elimination effects are synergistically exerted; the soil quality and the agricultural productivity are stably improved for a long time.

The invention discloses an artificial cyanobacteria crust inoculant as well as a preparation method and application thereof. The artificial cyanobacteria crust inoculant comprises a strain, a mixed matrix and water, the strain is composed of a blue-green algae group and a bacterial group; the mixed matrix comprises sandy soil and loam; the biomass of the blue-green algae group is 20-40 g / m < 2 >, and the biomass of the bacterial group is 150-250 g / m < 2 >. According to the method disclosed by the invention, the blue-green algae and the mixed strains are combined for use, so that the problems that a biological synergistic micro-ecological structure cannot be formed due to single biological composition of a traditional artificial blue-green algae crust inoculant, and ecological restoration of a sand area lags behind and is long in period are solved; the invention aims to construct a desert cyanobacteria crust inoculant formula adaptive to a compound biological group so as to accelerate ecological restoration of a desert area.

The invention discloses a probe combination, a chip, a kit and a method for detecting a floating hylothrix blue-green algae strain for producing microcystin. The floating hylothrix blue-green algae strain capable of producing the microcystic toxins comprises at least one of nine strains, and a probe combination comprises at least one of a probe group SEQ ID No.1 to 10, a probe group SEQ ID No.1 to 10, a probe group SEQ ID No.11 to 20, a probe group SEQ ID No.21 to 30, a probe group SEQ ID No.31 to 40, a probe group SEQ ID No.41 to 50, a probe group SEQ ID No.51 to 60, a probe group SEQ ID No.61 to 70, a probe group SEQ ID No.71 to 80 and a probe group SEQ ID No.81 to 90. The probe combination-based method for detecting the microcystin-producing floating hylothrix blue-green algae strain has the characteristics of fast time efficiency, high accuracy and high sensitivity through chip probe specific hybridization.

This invention discloses a genetically engineered Bacillus subtilis strain for producing Mycosporine-glycine, its construction method, and its applications. Starting with Bacillus subtilis WB600, this strain significantly enhances Mycosporine-glycine synthesis by knocking out the competing pathway gene ywjH and expressing the AvmysABC gene cluster from Anabaena polymorpha at multiple sites. Further knocking out the sporulation genes spo0A and spoIIIE and introducing the xylose metabolism gene clusters xylA-xylB, combined with a mixed carbon source fermentation strategy, significantly increased the yield. The engineered strain achieved a Mycosporine-glycine yield of 3.64 g / L in shake-flask fermentation and 15.8 g / L in a 50 L fed-batch fermenter.

The present application relates to a kind of nitrogen-fixing blue-green algae heteromorphosis and fast induction method and application, fast induction is first under the light condition, nitrogen-fixing blue-green algae is placed in nitrogen-deficient culture solution and is induced 12h, then the nitrogen-fixing blue-green algae taken out is placed in nitrogen-deficient phosphorus supply culture solution and continues to be induced 12h;Quantitative is carried out simultaneously to the number of heteromorphosis differentiated, cell size and relative fluorescence intensity using high content cell imaging analysis system;Compared with the induction in nitrogen-deficient culture solution without phosphorus supply, the number of heteromorphosis of three nitrogen-fixing blue-green algae increases by an average of 43.3%, wherein the number of heteromorphosis of anabaena increases by 116%;Nitrogen-deficient phosphorus supply culture mode can accelerate the process of inducing heteromorphosis differentiation, and it is found that under the condition of two-step total induction of 24 hours and 3 times of phosphorus supply, the number of heteromorphosis differentiated by three algae presents the best effect.

The present application relates to a method for improving the carbon and nitrogen content and the aggregate content of newly reclaimed farmland soil by using microalgae, which comprises: applying mixed algal liquid of chlorella and nitrogen-fixing anabaena to the soil. In order to solve the problem of low carbon and nitrogen content and low productivity of newly reclaimed farmland soil, the present application utilizes the ability of chlorella and nitrogen-fixing anabaena to synergistically and efficiently fix carbon dioxide in the air into organic carbon stored in the soil, and at the same time, through the biological nitrogen fixation effect, the nitrogen in the air is converted into nitrogen available to plants, thereby effectively improving the carbon and nitrogen retention capacity of the soil. In addition, the physiological activities of the two microalgae can also synergistically promote the formation of soil aggregates, improve the soil structure, and enhance the water and fertilizer retention capacity of the soil.