173 results about "Chlorella sp." patented technology

The invention discloses a bacterial strain for degrading aspartame, a bacterial-algae symbiotic system and application of the bacterial strain and the bacterial-algae symbiotic system, the bacterial strain is corn sphingomonas zeae NCU J1, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M 20251462. And an algal-bacterial symbiotic system is constructed by adopting an oriented symbiotic combination of the corn sphingomonas NCU J1 and the chlorella sp. The corn sphingomonas NCU J1 can metabolize an artificial sweetening agent aspartame and degradation products thereof, metabolism complementation can be achieved through an algal-bacterial symbiotic interface, and bacteria preferentially promote aspartame to be subjected to photohydrolysis to generate degradation products such as diketopiperazine derivatives. The microalgae synchronously start isomerism enzymatic reaction to convert degradation products such as diketopiperazine derivatives into low-toxicity products, the risk of secondary pollution is reduced by 40% or above compared with that of a traditional physical and chemical method, 150 mg / L aspartame can be efficiently degraded within 2 days, generation of derivatives of aspartame can be greatly reduced, secondary pollution is reduced, and the method is suitable for industrial production. And the method has a good application prospect in the aspect of rapidly treating aspartame-containing wastewater.

The invention relates to the technical field of aquaculture, in particular to an aquaculture water environment repairing agent and a preparation method thereof, and the repairing agent is composed of a modified biochar composite material, chlorella microspheres, a cross-linking agent and a microbial compound inoculant. The modified biochar composite material is prepared through the steps of mixing attapulgite and biomass raw materials, performing acid activation, performing pyrolysis and the like, and tannic acid is used for further modification so as to enhance the adsorption capacity; the chlorella microspheres are prepared from sodium alginate and a CaCl2 solution, and have good suspension property and stability; the microbial complex microbial inoculant contains various beneficial bacteria, and can decompose organic matters and reduce the content of ammonia nitrogen and nitrite; the components of the repairing agent are combined through the cross-linking agent to form particles, so that the repairing agent is convenient to put and recycle. The repairing agent disclosed by the invention has efficient pollutant removal capacity, and can be used for remarkably improving the water quality of aquatic water, improving the transparency of the water, increasing dissolved oxygen and promoting the health and stability of an aquatic ecosystem.

The invention discloses a phycomycete symbiotic system and application of the phycomycete symbiotic system in treatment of aquaculture tail water, and belongs to the technical field of tail water treatment. The phycomycete symbiotic system comprises chlorella, scenedesmus and activated sludge, and the initial inoculation dry weight ratio of the chlorella to the scenedesmus to the activated sludge is 1: 1: 6. And exogenously adding octanoyl-L-homoserine lactone or tetradecanoyl-L-homoserine lactone into the phycomycetes symbiotic system, so as to construct the phycomycetes symbiotic system with enhanced functions. On the basis of a mixed algae-bacteria symbiotic system with an optimized ratio, directional enhancement of functions is realized by virtue of quorum sensing signal molecules, the stability and resistance gene interception capability of the quorum sensing signal molecules under the stress of antibiotics are verified, and finally efficient purification of the aquaculture tail water is realized. According to the invention, the blank of the prior art in the aspects of advanced regulation and control of a phycomycete synergistic mechanism and synergistic treatment of emerging pollutants is filled up.

The invention discloses chlorella for efficiently removing eutrophic elements in sewage and application of the chlorella, and relates to the technical field of eutrophic sewage treatment. According to the invention, the chlorella sp. 111-4 is obtained through screening, and can efficiently remove eutrophic elements such as nitrogen, phosphorus and TOC in sewage; the chlorella sp. 111-4 is preserved in the China Center for Type Culture Collection, and the preservation number of the chlorella sp. 111-4 is CCTCC NO: M 2025475. The chlorella sp. 111-4 is inoculated into the sewage to be treated, the sewage treatment effect is more remarkable than that of other reported chlorella, and the degradation rate of nitrogen and phosphorus in the wastewater within 7 days reaches up to 97% or above.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

The invention discloses a method for treating pathogenic bacteria in reclaimed water by using biochar-microalgae biofilm particles. The method comprises the following steps: culturing chlorella, preparing the biochar-microalgae biofilm particles and adsorbing. The charcoal-microalgae biofilm particles can effectively fix microalgae and prevent the microalgae from running away from a reactor; after the treatment is completed, the microalgae and the wastewater are convenient to separate, and the separation cost is low; the activity of microalgae is high, the secretion of extracellular secretions is strong, and the overall treatment efficiency is high.

Provided are a modified-zeolite-immobilized algae ball, a preparation method therefor, and a use thereof. The modified-zeolite-immobilized algae ball uses sodium bicarbonate and chitosan to modify zeolite, and subsequently immobilizes the modified zeolite and chlorella in an embedding gel ball so as to achieve immobilization of the algae ball.

The invention belongs to the technical field of microorganisms, and discloses chlorella sorokiniana M6, application of the chlorella sorokiniana M6 and a method for synchronously synthesizing xanthophyll and protein. The preservation number of the Chlorella sorokiniana M6 provided by the invention is GDMCC NO.66205, the Chlorella sorokiniana M6 has an excellent capability of co-producing lutein and protein, and is almost free of chlorophyll synthesis, so that the Chlorella sorokiniana M6 has very important economic value and practical significance for realizing commercial application of microalgae protein and lutein production.

The invention provides a method for regulating and controlling growth and metabolism of caproic acid bacteria by using marine active peptides in a baijiu fermentation process. According to the method, the marine active peptides are added in an anaerobic fermentation stage of baijiu preparation to regulate and control the growth and metabolism of the caproic acid bacteria; wherein the marine active peptide is chlorella pyrenoidosa peptide, schizochytrium limacinum peptide, fish albumin peptide or euphausia superba peptide. According to the method, active peptides with specific sources are added into an anaerobic culture system, so that the growth of caproic acid bacteria can be promoted, energy metabolism can be enhanced, and the activity of key enzymes such as caproic acid dehydrogenase and caproic acid synthase can be activated, and therefore, the generation amount of flavor acids such as caproic acid and butyric acid can be increased, the fermentation quality can be improved, and the flavor level and esterification precursor substance content of Baijiu can be improved.

This invention belongs to the field of functional food processing technology, specifically relating to a method for improving the flavor of a chlorophyll-deficient Chlorella proteoglycan mutant. The method includes the following steps: S1, obtaining a Chlorella proteoglycan mutant strain; S2, centrifuging the Chlorella proteoglycan mutant strain and collecting the algal sludge, then pulverizing the collected algal sludge and dispersing it in water to obtain an algal powder suspension, then adding a compound enzyme to the algal powder suspension, and inactivating the enzyme after the reaction; S3, cooling the system to room temperature, adding glucose and stirring to dissolve, sterilizing, inoculating with a compound bacterial strain and fermenting, and sterilizing again after fermentation; S4, cooling the fermented system to room temperature, then adding β-cyclodextrin and stirring, and drying the system after stirring to obtain the algal protein product. This invention employs a synergistic process of compound enzymatic hydrolysis, compound bacterial fermentation, and β-cyclodextrin encapsulation to remove the off-flavor of the mutant algae from the source, significantly improving the flavor and enhancing the product's storage stability.

The invention provides a biological bait culture method for pond culture water. The biological bait culture method comprises the following steps: S1, building a three-dimensional culture system; s2, preparing an ecological base and a biocenosis; according to the method, by building a three-dimensional cultivation system of the bottom layer modular net cage, the water surface floating type three-dimensional cultivation frame and the intelligent regulation and control system and combining scientific configuration of ecological bases and biocenosis, the problem that bait supply is unstable in traditional cultivation is effectively solved; the light organism cultivation unit, the plant planting unit and the biological membrane carrier unit of the water surface floating type three-dimensional cultivation frame work in a layered mode, bait organisms such as chlorella, copepods and rotifers in different water layers are cultivated in a directional mode, a complete closed-loop food chain is constructed in addition to food filtering and nutrition supplementing of snails and freshwater mussels, and the bait yield per unit water body is greatly increased.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

The invention discloses chlorella capable of resisting acetate and urea and a breeding method and application thereof, and relates to the technical field of microorganisms, the chlorella is a Sorokiniana mutant strain, the classification name of the chlorella is Chlorella sorokiniana-TongWei-01, the chlorella is preserved in the China General Microbiological Culture Collection Center at 2025-12-1, and the preservation number of the chlorella is CGMCC NO. The address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46630. According to the method, large-scale culture can be performed by taking acetic acid / sodium acetate as a unique carbon source and urea as a unique nitrogen source, the method has excellent acetic acid utilization efficiency and urea tolerance, toxicity interference is resisted while two substrates are efficiently converted, high-density culture supported by a cheap carbon-nitrogen source is realized, and a core algae species support is provided for large-scale production of microalgae.

The present invention relates to the compositions comprising algae, specifically Spirulina platensis and Chlorella, and their use for the treatment of crops, such as fruits. vegetables and arable crops against heat stress, especially sunburn.

The invention provides a skin repairing composition for relieving red, itching and dry symptoms of sensitive skin and application, and belongs to the technical field of cosmetics. The composition comprises an effective amount of an extract of at least two algae selected from the group consisting of Phaeodactylum tricornutum, Spirulina, Chlorella and Micrococcus, and optionally comprises other skin repair ingredients cooperating therewith. The skin repair composition for relieving red, itching and dry symptoms of sensitive skin simultaneously and synergistically intervenes four major causes of sensitive skin formation, namely barrier damage, hyperneurosis, immune inflammation and micro-ecological imbalance, breaks vicious circle and establishes a repair closed loop by utilizing the multiple effects of specific algae active ingredients, and has the effects of relieving the symptoms of red, itching and dry symptoms of sensitive skin and relieving the symptoms of red, itching and dry symptoms of sensitive skin. Therefore, the problems of red skin, itching skin, dry skin and the like are radically relieved.

This invention relates to a method for immobilizing Chlorella, in order to... Chlorella Using *Chlorella sp. FACHB-9* as the culture medium, *Chlorella* solution was inoculated into the culture medium and cultured under conditions where light intensity varied with concentration to obtain a high-concentration *Chlorella* solution. Chitosan-modified rope-shaped artificial aquatic plants, used as an immobilization carrier, were added to the high-concentration *Chlorella* solution and fixed in a constant-temperature shaker at 25–35℃ and 120–180 rpm for 20–30 hours. After removal and drainage, immobilized *Chlorella* was obtained. An immobilized *Chlorella* was prepared by the above method. An application of the immobilized *Chlorella* was also described: the immobilized *Chlorella* was used to remove nitrogen and phosphorus from aquaculture wastewater. The beneficial effects included: effective removal of nitrogen and phosphorus pollution from aquaculture wastewater, prevention of eutrophication, reduction of wastewater treatment costs, inhibition of *Microcystis aeruginosa* growth, and creation of additional economic value through the resource utilization of *Chlorella*.

The invention provides a method for producing an extracellular polymeric substance by using an algal-bacterial symbiotic system, and belongs to the technical field of industrial biology. The method for producing the extracellular polymeric substance by utilizing the algal-bacterial symbiotic system comprises the following steps: inoculating chlorella pyrenoidosa and lactobacillus paracasei into a BG11 liquid culture medium, culturing under the conditions that the temperature is 27-29 DEG C, the rotating speed is 160-200rpm, the illumination intensity is 3800-4200LX and the illumination ratio is 24h: 0h, then respectively collecting supernate 1 and thalli, resuspending the thalli, collecting supernate 2, and carrying out freeze-drying to obtain the extracellular polymeric substance. Enabling the supernatant 1 and the supernatant 2 to pass through a filter membrane respectively, and collecting filtrate to obtain the traditional Chinese medicine composition. When the concentration of glucose in the G11 liquid culture medium is 3.8-4.2 g / L, the pH value is 4.7-5.2, and the ratio of bacteria to algae is (0.8-1.2): 1, the growth condition of the bacteria-algae symbiotic system is optimal, and when the concentration of sodium selenite is 2-4 mg / L, the yield of the extracellular polymeric substance is highest, and efficient preparation of the extracellular polymeric substance is realized.

The application discloses a kind of preparation methods of bacteria-algae symbiosis filler, specifically: to the reactor filled with filler, add active sludge dispersion liquid, under aeration condition, 7-10 days are operated, and simulate tail water is passed through during operation process;Then inoculate chlorella according to the volume ratio of active sludge dispersion liquid and chlorella dispersion liquid 1:5-50, 22-30 DEG C and under illumination, 14-20 days are operated, wherein, light dark ratio is: illumination 6-12h, dark 12-18h;Simulate sewage plant tail water is passed through during operation process;Get the filler that hangs bacteria-algae symbiosis biofilm.The bacteria-algae symbiosis film formed in the application, microalgae can promote the enrichment of denitrifying bacteria and organic matter metabolic functional bacteria, and bacteria can improve the photosynthesis performance and carbon fixation performance of microalgae through material exchange and information transmission, so that the bacteria-algae symbiotic system can efficiently purify N / P / organic matter in water body, while reducing greenhouse gas emissions.The application also discloses a filler combination structure based on the above bacteria-algae symbiotic filler.

The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have wide application prospects.

The invention discloses an anti-oxidation and anti-aging composition and application thereof.The anti-oxidation and anti-aging composition comprises a chlorella extract and a rice chaff extract, practice shows that after the chlorella extract and the rice chaff extract are combined, the anti-oxidation and anti-aging effect can be achieved, and the obvious amplification can be achieved in an anti-oxidation test-DPPH free radical scavenging experiment; and after combination, no negative influence is caused on the activity of the fibroblast, and the expression level of the COL I of the fibroblast is obviously improved. In addition, an obvious effect can be achieved under the condition of low concentration, large-amount addition is not needed, and the effect is stable.

This invention discloses a method for isolating and preparing Chlorella exovesicles. Specifically, Chlorella, conventionally cultured to the early logarithmic growth phase, is induced to develop in a nitrogen-limited medium. The culture medium is then concentrated using a tangential flow filtration system. Finally, the concentrate is subjected to density gradient centrifugation with iodixanol. After centrifugation, components within the density range of 1.10–1.12 g / mL are collected to obtain the Chlorella exovesicle product. This invention achieves concentrated and precise collection of Chlorella exovesicles by regulating membrane lipids during the early induction culture of Chlorella, combined with TFF concentration and density gradient centrifugation. This method effectively eliminates impurities, achieving a recovery rate of over 60% and a purity of 2.56 × 10⁻⁶. 10 The particle / μg method provides an efficient, controllable, and scalable solution for the industrial-scale separation and preparation of microalgal exovesicles.

The utility model relates to a park landscape system based on ecological interaction type of chlorella micro-aquatic ecosystem, it is to solve the technical problem of the poor interactivity function of existing intelligent park, the park landscape system of the utility model includes pool, dot matrix light source, energy storage battery, motion power generation facility, solar panel, photosynthesis monitor, controller, the small chlorella is put in the pool, the dot matrix light source is laid in the pool bottom, and the solar panel, motion power generation facility is connected with energy storage battery respectively, and the energy storage battery is connected with the dot matrix light source, and the photosynthesis monitor is set in the pool top, and the small chlorella growth state in the pool is monitored and is sent to the controller, and the controller is also connected with the dot matrix light source, controls the light mode and the light intensity of each light point in the dot matrix light source. Participants carry out the claim to the small chlorella in the interactive pool through the interactive applet on the controller of mobile phone scanning controller, and send control command, participate in the landscape formation in the pool, can be used in the field of city park.

The invention belongs to the technical field of soil remediation, and relates to a microalgae-microorganism compound preparation for improving saline-alkali soil and application. The microalgae-microorganism compound preparation is prepared from spirulina, chlorella, nostoc, scenedesmus obliquus, bacillus mucilaginosus, bacillus mucilaginosus and pseudomonas. The microalgae-microorganism compound preparation for improving the saline-alkali soil can improve the available phosphorus content of the soil, increase the available potassium content of the soil and effectively improve the soil; soil bulk density is reduced, and soil hardening is improved; therefore, the fertilizer retention property of the soil is enhanced, the growth and development of crop roots are facilitated, the crop yield is remarkably improved, and the application prospect is wide.

The invention belongs to the technical field of electrocatalysis, and discloses a chlorella electrode with redox activity and a preparation method and application thereof. Under a constant potential window, methylene blue, aniline and chlorella are synchronously subjected to electrochemical oxidation to generate free radicals, an electron transport chain between chlorella and methylene blue is constructed through a click reaction, meanwhile, an interpenetrating network structure copolymer is generated in the copolymerization process, and chlorella is wrapped and anchored on the surface of an electrode. According to the method, chlorella can be effectively dispersed, more active sites are exposed, the electron transfer efficiency is improved, and the stability of the electrode is enhanced under the interaction of the three. The obtained chlorella electrode with redox activity has an application prospect in the field of electrocatalytic reduction of carbon dioxide into high-added-value products.

The invention belongs to the technical field of antibacterial hydrogel preparation, and particularly relates to antioxidant antibacterial hydrogel as well as a preparation method and application thereof. The method comprises the following steps: dispersing chitosan in water, then adding glacial acetic acid for reaction, adding 2, 3-epoxypropyltrimethylammonium chloride for continuous reaction after the reaction is finished, centrifuging after the reaction is finished to obtain supernate, and then adding a mixed solution of acetone and ethanol for precipitation, suction filtration and freeze drying to obtain chitosan quaternary ammonium salt; the preparation method comprises the following steps: dissolving chitosan quaternary ammonium salt in water, adding Pluronic F-127, stirring until the Pluronic F-127 is completely dissolved, adding the freeze-dried chlorella powder, and uniformly stirring to obtain the antioxidant and antibacterial hydrogel. The characteristics of the wound microenvironment are improved through the freeze-dried chlorella, and the freeze-dried chlorella is cooperated with the antibacterial hydrogel in function, so that an efficient treatment effect is achieved; meanwhile, the preparation process is simple, the raw material source is wide, and the clinical application prospect is good.

The present application relates to a kind of chlorella-nanose complex hydrogel and its preparation method and application, belong to nanose antibacterial technical field.The chlorella-nanose complex hydrogel includes hydrogel matrix, and the chlorella and photo-thermal nanose loaded on the hydrogel matrix.The present application also provides the preparation method of the complex hydrogel, and the application in the preparation of antibacterial drug or dressing and the killing gram-negative bacteria and gram-positive bacteria.The present application combines chlorella and nanose for the first time, and constructs a self-oxygen supply antibacterial platform.The chlorella in the chlorella-nanose complex hydrogel can carry out photosynthesis under illumination, continuously produce oxygen, provide sufficient substrate for nanose oxidase-like reaction, overcome the low activity defect of traditional nanose in anoxic environment.

The present application relates to the technical field of aquatic environment, and in particular to a lactic acid bacteria, a separation and screening method and application, wherein the lactic acid bacteria is Lacticaseibacillus casei FC-5 with a preservation number of CCTCC NO: M 2026260, and the present application further provides a method for application of the lactic acid bacteria and chlorella in bio-floc technology, and solves the problem of serious threat of accumulation of nitrite nitrogen to aquatic animals in aquatic environment, and also solves the problem of serious threat of accumulation of ammonium nitrogen to aquatic animals.

The utility model relates to the technical field of bioengineering, and discloses an in-situ generating device convenient for collecting chlorella, which comprises a culture room, a feed pipe fixedly mounted on the upper surface of the culture room, a culture solution tank fixedly mounted at the end of the feed pipe, a water tank fixedly mounted on the culture solution tank, and a water outlet fixedly mounted on the culture solution tank. A culture pond is fixedly installed on the inner wall of the culture room, a controller is fixedly installed on the inner wall of the culture room, and a water supplementing pond is fixedly connected to the outer side of the culture pond. According to the in-situ generation device facilitating chlorella collection, by arranging the collection mechanism, during collection, a sealing cover on a culture pond can be opened, a telescopic air cylinder is started to drive a collection net plate to move, a cleaning plate is better attached to the inner wall of the culture pond under the elastic force action of a volute spiral spring, a guide cylinder is further arranged on the outer side of the cleaning plate, and the collection net plate can be conveniently cleaned; and meanwhile, the cleaning plate is detachably mounted, so that the chlorella can be better collected after being collected.

The invention discloses a method and a device for treating kitchen waste fermentation wastewater containing stress-resistant chlorella, and belongs to the technical field of microbiological treatment of wastewater. According to the invention, chlorella ZJU-MWZ003 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC NO.46394 or chlorella ZJU-MWZ019 which is preserved in the China General Microbiological Culture Collection Center (CGMCC) and has the preservation number of CGMCC NO.46395 is adopted. The sodium acetate-resistant chlorella strain is activated and cultured in a BG11 culture medium to a logarithmic phase, and then the strain is inoculated into pretreated kitchen waste fermentation wastewater for treatment. The treatment operation process is simple, the technical threshold is low, and industrial application is better facilitated.

The application discloses a preparation method of mycelial pellet composite material for enhanced denitrification of micro-polluted water, and comprises the following steps: screening and obtaining a bacterial strain and a fungal strain, and preparing a bacterial suspension; culturing the fungal strain to prepare mycelial pellets; culturing the bacterial suspension in a mycelial pellet culture medium to prepare DMP; modifying biochar by KMnO4 to prepare modified biochar, and preparing a modified biochar suspension; adding the modified biochar suspension and a chlorella suspension into the DMP, and oscillating and culturing to prepare the mycelial pellet composite material. The mycelial pellet composite material for enhanced denitrification of micro-polluted water prepared by the application has the characteristics of structural stability, high electron transfer efficiency and strong adaptability.