224 results about "Chlorella sp." patented technology

The invention discloses a chlorella microalgae and an application of the chlorella microalgae in sewage treatment, and the collection number of the chlorella is CCTCC (China Center for Type Culture Collection) 20231952. Chlorella is used for treating sewage, and for oil-containing food processing plant wastewater, compared with activated sludge, chlorella has no growth inhibition on high-oil wastewater. For printing and dyeing wastewater which cannot be treated by activated sludge, chlorella can be used for directly treating the printing and dyeing wastewater. The treatment of animal husbandry wastewater shows that the chlorella further degrades refractory organic matters (COD (Chemical Oxygen Demand) is reduced to 200mg / L or below) in the sewage and can replace an advanced treatment end, namely, the dosage of chemical agents is reduced, the secondary pollution is avoided, and the cost is saved. Meanwhile, the treatment conditions of anaerobic effluent and raw wastewater show that compared with an activated sludge method and an anaerobic digestion method, the chlorella can more quickly and efficiently remove organic matters in sewage.

The invention discloses a preparation method of microalgae nano-selenium and application of biotransformed nano-selenium in prevention and treatment of plant late blight. The novel preparation method of the microalgae nano-selenium comprises the following steps: firstly, extracting an intracellular extract of chlorella pyrenoidosa, and then adding sodium selenite into the intracellular extract to carry out nano-selenium conversion; compared with an existing reported method that after sodium selenite is added into a microalgae living body culture solution to be converted into nano-selenium, algae is taken to break cells, and the nano-selenium is collected, the method has the advantages that the microalgae living body does not need to be used when the nano-selenium is biologically converted, and the subsequent complicated nano-selenium separation and purification steps can be avoided; the nano-selenium obtained by converting the intracellular extract can be directly applied. Experiments prove that the effect of the nano-selenium obtained through biotransformation on inhibiting phytophthora infestans of potato late blight pathogenic bacteria is obviously better than that of chemically synthesized nano-selenium, and the nano-selenium obtained through biotransformation has an obvious effect on preventing and treating potato late blight and has an obvious effect on promoting plant growth.

The invention discloses a bacterial strain for degrading aspartame, a bacterial-algae symbiotic system and application of the bacterial strain and the bacterial-algae symbiotic system, the bacterial strain is corn sphingomonas zeae NCU J1, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M 20251462. And an algal-bacterial symbiotic system is constructed by adopting an oriented symbiotic combination of the corn sphingomonas NCU J1 and the chlorella sp. The corn sphingomonas NCU J1 can metabolize an artificial sweetening agent aspartame and degradation products thereof, metabolism complementation can be achieved through an algal-bacterial symbiotic interface, and bacteria preferentially promote aspartame to be subjected to photohydrolysis to generate degradation products such as diketopiperazine derivatives. The microalgae synchronously start isomerism enzymatic reaction to convert degradation products such as diketopiperazine derivatives into low-toxicity products, the risk of secondary pollution is reduced by 40% or above compared with that of a traditional physical and chemical method, 150 mg / L aspartame can be efficiently degraded within 2 days, generation of derivatives of aspartame can be greatly reduced, secondary pollution is reduced, and the method is suitable for industrial production. And the method has a good application prospect in the aspect of rapidly treating aspartame-containing wastewater.

The invention discloses an EMS-ARTP compound mutation chlorella pyrenoidosa mutant strain and an application thereof. The preparation method comprises the following steps: mutagenizing a chlorella pyrenoidosa mutant strain CX41 by adopting an ethyl methanesulfonate (EMS) and normal-pressure and room-temperature plasma (ARTP) composite technology, screening and verifying to obtain a golden yellow chlorella pyrenoidosa mutant strain, naming the golden yellow chlorella pyrenoidosa mutant strain as chlorella pyrenoidosa Y11, and screening and verifying the golden yellow chlorella pyrenoidosa Y11 to obtain the chlorella pyrenoidosa Y11. The strain is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on the fifth floor of the building 59, No. 100 courtyard, Xianlie Middle Road, Guangzhou on October 23, 2024, and the preservation number is GDMCC No: 65324. The chlorella pyrenoidosa mutant strain Y11 has the characteristics of high yield of protein and rich carotenoids, and can be used for fermentation production of microalgae protein and carotenoids.

The invention provides a chlorella pyrenoidosa calcium chelating peptide and a chlorella pyrenoidosa peptide calcium chelate prepared by using the chlorella pyrenoidosa calcium chelating peptide. The prepared chlorella pyrenoidosa peptide calcium chelate can enhance the maximum load and structural strength of the tibia of a mouse with osteoporosis. The chlorella pyrenoidosa calcium chelating peptide provided by the invention is combined with calcium ions to form a peptide-calcium chelate, and animal experiments prove that the peptide-calcium chelate can significantly improve the maximum load and structural strength of the tibia of a mouse with osteoporosis, and has a good effect of improving the bone biomechanical properties of the mouse with osteoporosis.

The invention discloses a chlorella pyrenoidosa white mutant strain FZU65-W1 and application of the chlorella pyrenoidosa white mutant The classification name of the chlorella pyrenoidosa white mutant strain FZU65-W1 is chlorella pyrenoidosa, the chlorella pyrenoidosa white mutant strain is preserved in the China General Microbiological Culture Collection Center on September 11, 2024, the preservation number is CGMCC N0.46151, and the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing. Compared with an original strain FZU65, the chlorella pyrenoidosa white mutant strain FZU65-W1 has the advantages that the growth speed of algae cells under the heterotrophic condition is higher, and meanwhile, the cells are white due to the fact that chlorophyll and carotenoid are not synthesized in the cells. The chlorella pyrenoidosa white mutant strain FZU65-W1 can be used for producing high-quality protein through heterotrophic fermentation, so that not only can a later-stage protein extraction and preparation process be simplified, but also the sensory acceptance of algae powder can be improved, and a high-quality germplasm resource is provided for industrial production and application of microalgae protein.

The invention particularly relates to a method for cultivating biological bait in an outdoor pond. According to the method, after an outdoor pond is disinfected and cleaned, a biological fertilizer source and various beneficial bacteria combination are used in a combined mode, cryptococcus ovale, composite diatom and chlorella are inoculated, a stable pond bacterium-algae system is constructed, and various cultivated microalgae are used for strengthening zooplanktons of rotifers, cladocerans and copepods in the bait pond. The breeding period of the biological bait is shortened, the survival rate of the biological bait is increased, the yield of the biological bait is increased, meanwhile, the nutritional value of the biological bait is enhanced, and opening breeding of the fry is facilitated. The breeding method is low in cost and suitable for various weathers, and sufficient bait guarantee is provided for large-scale breeding of fish fries.

The invention relates to the technical field of microalgae heterotrophic culture and biomass proliferation, in particular to a chlorella pyrenoidosa heterotrophic high-density proliferation method. The specific technical scheme is as follows: the heterotrophic high-density proliferation method of chlorella pyrenoidosa comprises the following steps: pre-culture and heterotrophic propagation of chlorella pyrenoidosa: inoculating a chlorella pyrenoidosa solution into a BG11 culture medium, and culturing for 3-4 days in a constant-temperature shaking table with the temperature of 25 + / -1 DEG C, the rotating speed of 200rpm and the light-dark ratio of 0h: 24h to obtain a primary culture chlorella solution; inoculating the primary culture algae liquid into a pre-sterilized and optimized heterotrophic culture medium, and carrying out expanding culture under the same culture condition for 3-4 days to obtain a secondary expanding culture algae liquid; (2) a heterotrophic high-density culture stage: regularly supplementing a nutrient culture medium containing high-concentration glucose, nitrate and phosphate by adopting an index fed-batch feeding strategy; other conditions are kept consistent during culture; and (3) an in-situ protein accumulation induction stage: when the chlorella pyrenoidosa reaches an exponential growth end stage, adding paclobutrazol into the system, and keeping other conditions consistent while keeping exponential fed-batch culture. According to the method disclosed by the invention, the biomass of the chlorella pyrenoidosa is increased by about 128.6% compared with that of the traditional batch culture, the protein content can reach 49.14%, meanwhile, the configuration of essential amino acids is improved, and the requirements of high-end biomass resource application are met. The method has the advantages of simplicity and convenience in operation and high production efficiency, and is suitable for the fields of high-quality protein extraction and related biomass production.

The invention relates to the technical field of aquaculture, in particular to an aquaculture water environment repairing agent and a preparation method thereof, and the repairing agent is composed of a modified biochar composite material, chlorella microspheres, a cross-linking agent and a microbial compound inoculant. The modified biochar composite material is prepared through the steps of mixing attapulgite and biomass raw materials, performing acid activation, performing pyrolysis and the like, and tannic acid is used for further modification so as to enhance the adsorption capacity; the chlorella microspheres are prepared from sodium alginate and a CaCl2 solution, and have good suspension property and stability; the microbial complex microbial inoculant contains various beneficial bacteria, and can decompose organic matters and reduce the content of ammonia nitrogen and nitrite; the components of the repairing agent are combined through the cross-linking agent to form particles, so that the repairing agent is convenient to put and recycle. The repairing agent disclosed by the invention has efficient pollutant removal capacity, and can be used for remarkably improving the water quality of aquatic water, improving the transparency of the water, increasing dissolved oxygen and promoting the health and stability of an aquatic ecosystem.

The invention belongs to the technical field of water treatment, and particularly relates to activated carbon with high adsorption capacity. The chlorella protein nucleus is selected as a carbon source, and through secondary activation, the pore structure of the activated carbon is more developed, and the pore size distribution is more concentrated, so that the activated carbon is endowed with better performance.

The invention discloses a phycomycete symbiotic system and application of the phycomycete symbiotic system in treatment of aquaculture tail water, and belongs to the technical field of tail water treatment. The phycomycete symbiotic system comprises chlorella, scenedesmus and activated sludge, and the initial inoculation dry weight ratio of the chlorella to the scenedesmus to the activated sludge is 1: 1: 6. And exogenously adding octanoyl-L-homoserine lactone or tetradecanoyl-L-homoserine lactone into the phycomycetes symbiotic system, so as to construct the phycomycetes symbiotic system with enhanced functions. On the basis of a mixed algae-bacteria symbiotic system with an optimized ratio, directional enhancement of functions is realized by virtue of quorum sensing signal molecules, the stability and resistance gene interception capability of the quorum sensing signal molecules under the stress of antibiotics are verified, and finally efficient purification of the aquaculture tail water is realized. According to the invention, the blank of the prior art in the aspects of advanced regulation and control of a phycomycete synergistic mechanism and synergistic treatment of emerging pollutants is filled up.

According to the chlorella pyrenoidosa high-F-value peptide and the preparation method thereof, parameters in the enzymolysis and adsorption processes are strictly controlled, and the enzymolysis of the incision enzyme and the excision enzyme not only can efficiently hydrolyze chlorella protein and generate more peptide fragments with biological activity, but also can be used for preparing the chlorella pyrenoidosa high-F-value peptide. And the characteristics of high industrial production cost and poor stability caused by wide enzyme cutting sites are solved. The prepared chlorella pyrenoidosa high-F-value peptide reaches 36.90 + / -8.18, the F value of the chlorella pyrenoidosa high-F-value peptide is increased by 12.72 times compared with the chlorella pyrenoidosa raw material (2.90 + / -0.07), and in addition, the molecular weight of the high-F-value oligopeptide is 1t; peptides with the molecular weight of 1000 Da account for 95-99.40%, the characteristics of oligopeptides are met, the molecular weight is small, and digestion and absorption are easy. The prepared high-F-value oligopeptide can accelerate metabolism of ethyl alcohol in the liver and protect the liver from being damaged by the ethyl alcohol, and has the effects of dispelling the effects of alcohol and protecting the liver.

The invention discloses chlorella for efficiently removing eutrophic elements in sewage and application of the chlorella, and relates to the technical field of eutrophic sewage treatment. According to the invention, the chlorella sp. 111-4 is obtained through screening, and can efficiently remove eutrophic elements such as nitrogen, phosphorus and TOC in sewage; the chlorella sp. 111-4 is preserved in the China Center for Type Culture Collection, and the preservation number of the chlorella sp. 111-4 is CCTCC NO: M 2025475. The chlorella sp. 111-4 is inoculated into the sewage to be treated, the sewage treatment effect is more remarkable than that of other reported chlorella, and the degradation rate of nitrogen and phosphorus in the wastewater within 7 days reaches up to 97% or above.

The present invention provides an essence containing chlorella extract and a preparation method thereof. The chlorella extract process comprises the following steps: raw material pulverization; enzymatic hydrolysis with cellulase and trypsin; primary fermentation using a mixture of Bacillus natto and Bacillus pumilus; addition of bamboo leaf extract, corn steep liquor, and sucrose, followed by inoculation with Rhodotorula glutinosus for secondary fermentation to obtain the chlorella extract. The mixed fermentation of multiple strains exhibits a synergistic effect, resulting in greater enrichment of natural products during the fermentation process compared to single-strain fermentation. Furthermore, the present invention provides an essence containing chlorella extract, glycerin, sodium hyaluronate, honey, and water, which exhibits significant moisturizing, antioxidant, and soothing effects.

The invention belongs to the technical field of biological culture, and particularly relates to a chlorella extracellular fluid and a preparation method and application thereof.The preparation method of the chlorella extracellular fluid comprises the following steps that a BG11 culture medium is inoculated with a chlorella solution cultured to the logarithmic phase, oxcarbazepine is added, and the chlorella extracellular fluid is obtained; the preparation method comprises the following steps: placing chlorella in an intermittent illumination environment with the temperature of 20-30 DEG C and the rotating speed of 100-200 rpm for growth, centrifuging the chlorella to obtain supernate after the chlorella grows to the 10th-16th day, filtering, sub-packaging, and performing cryopreservation to obtain the chlorella extracellular fluid. By adding oxcarbazepine, the chlorella extracellular fluid prepared in a specific culture medium and a specific growth environment has high oxidation activity, excellent stability and excellent degradation capacity, and a new thought is provided for solving the pollution treatment problem of current environmental pollutants such as pharmaceutical products, polycyclic aromatic hydrocarbons and dyes.

The invention discloses a multi-size aquatic organism bait culture method, which is characterized in that livestock and poultry waste extract liquid prepared by the culture method is used for co-culture of Rhodospiraceae bacteria and Chlorella algae, and the obtained algae is used for co-culture of zooplankton of Brachionidae and Harpacidae to finally obtain the multi-size aquatic organism bait. Compared with the harpacticoida cultured only by algae, the harpacticoida obtained by the culture method has the advantages that the EPA content is increased by 90.53%, the DHA content is increased by 13.47%, and the nutritional value is higher; a reasonable production system provides bait with multiple sizes, and the yield of chlorella algae, brachionus and harpacticoida is effectively increased; moreover, a new way for resource utilization of livestock wastes is provided, and efficient, environment-friendly and sustainable bait production is realized.

The invention provides an immobilized algae ball, a preparation method thereof and an immobilized algae ball and bacterium symbiotic system, and belongs to the technical field of sewage treatment, and the preparation method comprises the following steps: taking a chlorella culture solution, centrifuging to obtain concentrated chlorella, mixing the concentrated chlorella with deionized water to obtain an algae solution, preparing a sodium alginate solution at the same time, and adding the sodium alginate solution into the algae solution; uniformly mixing the algae solution with the sodium alginate solution to obtain a mixed solution; preparing a CaCl2 solution, slowly dropwise adding the mixed solution into the CaCl2 solution, carrying out immobilized cross-linking at room temperature for 23-25 hours, and washing with deionized water to obtain the algae balls. The algae balls are prepared by adopting a sodium alginate-calcium chloride embedding method, the concentration and crosslinking time of sodium alginate and calcium chloride are optimized, the immobilization of chlorella is realized, nitrogen and phosphorus in sewage can be effectively removed by the algae balls and a suspended sludge system, and the algae balls can be recycled after sewage treatment.

The invention discloses a method for treating pathogenic bacteria in reclaimed water by using biochar-microalgae biofilm particles. The method comprises the following steps: culturing chlorella, preparing the biochar-microalgae biofilm particles and adsorbing. The charcoal-microalgae biofilm particles can effectively fix microalgae and prevent the microalgae from running away from a reactor; after the treatment is completed, the microalgae and the wastewater are convenient to separate, and the separation cost is low; the activity of microalgae is high, the secretion of extracellular secretions is strong, and the overall treatment efficiency is high.

Provided are a modified-zeolite-immobilized algae ball, a preparation method therefor, and a use thereof. The modified-zeolite-immobilized algae ball uses sodium bicarbonate and chitosan to modify zeolite, and subsequently immobilizes the modified zeolite and chlorella in an embedding gel ball so as to achieve immobilization of the algae ball.

The invention belongs to the technical field of microorganisms, and discloses chlorella sorokiniana M6, application of the chlorella sorokiniana M6 and a method for synchronously synthesizing xanthophyll and protein. The preservation number of the Chlorella sorokiniana M6 provided by the invention is GDMCC NO.66205, the Chlorella sorokiniana M6 has an excellent capability of co-producing lutein and protein, and is almost free of chlorophyll synthesis, so that the Chlorella sorokiniana M6 has very important economic value and practical significance for realizing commercial application of microalgae protein and lutein production.

The invention relates to a chlorella culture method which comprises the following steps: carrying out first culture on a first chlorella species to obtain a first culture solution; performing second culture on a second chlorella strain in the presence of the first culture solution to obtain a second culture solution; mixing a flue gas absorption solution with the second culture solution, and carrying out third culture to obtain a third culture solution; wherein the flue gas absorption liquid contains hydrogen peroxide and nitric acid. According to the invention, inorganic nitrogen in the flue gas absorption liquid can be fixed into the chlorella biomass, the chlorophyll content of chlorella is obviously improved, and the utilization value of chlorella is improved.

The invention provides a method for regulating and controlling growth and metabolism of caproic acid bacteria by using marine active peptides in a baijiu fermentation process. According to the method, the marine active peptides are added in an anaerobic fermentation stage of baijiu preparation to regulate and control the growth and metabolism of the caproic acid bacteria; wherein the marine active peptide is chlorella pyrenoidosa peptide, schizochytrium limacinum peptide, fish albumin peptide or euphausia superba peptide. According to the method, active peptides with specific sources are added into an anaerobic culture system, so that the growth of caproic acid bacteria can be promoted, energy metabolism can be enhanced, and the activity of key enzymes such as caproic acid dehydrogenase and caproic acid synthase can be activated, and therefore, the generation amount of flavor acids such as caproic acid and butyric acid can be increased, the fermentation quality can be improved, and the flavor level and esterification precursor substance content of Baijiu can be improved.

This invention belongs to the field of functional food processing technology, specifically relating to a method for improving the flavor of a chlorophyll-deficient Chlorella proteoglycan mutant. The method includes the following steps: S1, obtaining a Chlorella proteoglycan mutant strain; S2, centrifuging the Chlorella proteoglycan mutant strain and collecting the algal sludge, then pulverizing the collected algal sludge and dispersing it in water to obtain an algal powder suspension, then adding a compound enzyme to the algal powder suspension, and inactivating the enzyme after the reaction; S3, cooling the system to room temperature, adding glucose and stirring to dissolve, sterilizing, inoculating with a compound bacterial strain and fermenting, and sterilizing again after fermentation; S4, cooling the fermented system to room temperature, then adding β-cyclodextrin and stirring, and drying the system after stirring to obtain the algal protein product. This invention employs a synergistic process of compound enzymatic hydrolysis, compound bacterial fermentation, and β-cyclodextrin encapsulation to remove the off-flavor of the mutant algae from the source, significantly improving the flavor and enhancing the product's storage stability.

The invention provides a biological bait culture method for pond culture water. The biological bait culture method comprises the following steps: S1, building a three-dimensional culture system; s2, preparing an ecological base and a biocenosis; according to the method, by building a three-dimensional cultivation system of the bottom layer modular net cage, the water surface floating type three-dimensional cultivation frame and the intelligent regulation and control system and combining scientific configuration of ecological bases and biocenosis, the problem that bait supply is unstable in traditional cultivation is effectively solved; the light organism cultivation unit, the plant planting unit and the biological membrane carrier unit of the water surface floating type three-dimensional cultivation frame work in a layered mode, bait organisms such as chlorella, copepods and rotifers in different water layers are cultivated in a directional mode, a complete closed-loop food chain is constructed in addition to food filtering and nutrition supplementing of snails and freshwater mussels, and the bait yield per unit water body is greatly increased.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

The invention discloses chlorella capable of resisting acetate and urea and a breeding method and application thereof, and relates to the technical field of microorganisms, the chlorella is a Sorokiniana mutant strain, the classification name of the chlorella is Chlorella sorokiniana-TongWei-01, the chlorella is preserved in the China General Microbiological Culture Collection Center at 2025-12-1, and the preservation number of the chlorella is CGMCC NO. The address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46630. According to the method, large-scale culture can be performed by taking acetic acid / sodium acetate as a unique carbon source and urea as a unique nitrogen source, the method has excellent acetic acid utilization efficiency and urea tolerance, toxicity interference is resisted while two substrates are efficiently converted, high-density culture supported by a cheap carbon-nitrogen source is realized, and a core algae species support is provided for large-scale production of microalgae.

The present invention relates to the compositions comprising algae, specifically Spirulina platensis and Chlorella, and their use for the treatment of crops, such as fruits. vegetables and arable crops against heat stress, especially sunburn.

The invention provides a skin repairing composition for relieving red, itching and dry symptoms of sensitive skin and application, and belongs to the technical field of cosmetics. The composition comprises an effective amount of an extract of at least two algae selected from the group consisting of Phaeodactylum tricornutum, Spirulina, Chlorella and Micrococcus, and optionally comprises other skin repair ingredients cooperating therewith. The skin repair composition for relieving red, itching and dry symptoms of sensitive skin simultaneously and synergistically intervenes four major causes of sensitive skin formation, namely barrier damage, hyperneurosis, immune inflammation and micro-ecological imbalance, breaks vicious circle and establishes a repair closed loop by utilizing the multiple effects of specific algae active ingredients, and has the effects of relieving the symptoms of red, itching and dry symptoms of sensitive skin and relieving the symptoms of red, itching and dry symptoms of sensitive skin. Therefore, the problems of red skin, itching skin, dry skin and the like are radically relieved.

This invention relates to a method for immobilizing Chlorella, in order to... Chlorella Using *Chlorella sp. FACHB-9* as the culture medium, *Chlorella* solution was inoculated into the culture medium and cultured under conditions where light intensity varied with concentration to obtain a high-concentration *Chlorella* solution. Chitosan-modified rope-shaped artificial aquatic plants, used as an immobilization carrier, were added to the high-concentration *Chlorella* solution and fixed in a constant-temperature shaker at 25–35℃ and 120–180 rpm for 20–30 hours. After removal and drainage, immobilized *Chlorella* was obtained. An immobilized *Chlorella* was prepared by the above method. An application of the immobilized *Chlorella* was also described: the immobilized *Chlorella* was used to remove nitrogen and phosphorus from aquaculture wastewater. The beneficial effects included: effective removal of nitrogen and phosphorus pollution from aquaculture wastewater, prevention of eutrophication, reduction of wastewater treatment costs, inhibition of *Microcystis aeruginosa* growth, and creation of additional economic value through the resource utilization of *Chlorella*.

The present invention provides a practical method for preparing a chlorella culture medium, wherein anhydrous sodium acetate, soil leaching solution, jackfruit leaf extract, oxalis leaf extract, macronutrient water-soluble fertilizer and tracenutrient water-soluble fertilizer are mixed to prepare the chlorella culture medium. The chlorella culture medium of the present invention is rich in various nutrients and growth factors, can ensure the rapid growth requirements of chlorella, has a strong inhibitory effect on bacteria and parasites, can effectively reduce the contamination of miscellaneous bacteria and parasites during the chlorella culture process, and has obvious effects.