22 results about "Chlorella sp." patented technology

This invention belongs to the field of functional food processing technology, specifically relating to a method for improving the flavor of a chlorophyll-deficient Chlorella proteoglycan mutant. The method includes the following steps: S1, obtaining a Chlorella proteoglycan mutant strain; S2, centrifuging the Chlorella proteoglycan mutant strain and collecting the algal sludge, then pulverizing the collected algal sludge and dispersing it in water to obtain an algal powder suspension, then adding a compound enzyme to the algal powder suspension, and inactivating the enzyme after the reaction; S3, cooling the system to room temperature, adding glucose and stirring to dissolve, sterilizing, inoculating with a compound bacterial strain and fermenting, and sterilizing again after fermentation; S4, cooling the fermented system to room temperature, then adding β-cyclodextrin and stirring, and drying the system after stirring to obtain the algal protein product. This invention employs a synergistic process of compound enzymatic hydrolysis, compound bacterial fermentation, and β-cyclodextrin encapsulation to remove the off-flavor of the mutant algae from the source, significantly improving the flavor and enhancing the product's storage stability.

This invention relates to a method for immobilizing Chlorella, in order to... Chlorella Using *Chlorella sp. FACHB-9* as the culture medium, *Chlorella* solution was inoculated into the culture medium and cultured under conditions where light intensity varied with concentration to obtain a high-concentration *Chlorella* solution. Chitosan-modified rope-shaped artificial aquatic plants, used as an immobilization carrier, were added to the high-concentration *Chlorella* solution and fixed in a constant-temperature shaker at 25–35℃ and 120–180 rpm for 20–30 hours. After removal and drainage, immobilized *Chlorella* was obtained. An immobilized *Chlorella* was prepared by the above method. An application of the immobilized *Chlorella* was also described: the immobilized *Chlorella* was used to remove nitrogen and phosphorus from aquaculture wastewater. The beneficial effects included: effective removal of nitrogen and phosphorus pollution from aquaculture wastewater, prevention of eutrophication, reduction of wastewater treatment costs, inhibition of *Microcystis aeruginosa* growth, and creation of additional economic value through the resource utilization of *Chlorella*.

This invention discloses a method for isolating and preparing Chlorella exovesicles. Specifically, Chlorella, conventionally cultured to the early logarithmic growth phase, is induced to develop in a nitrogen-limited medium. The culture medium is then concentrated using a tangential flow filtration system. Finally, the concentrate is subjected to density gradient centrifugation with iodixanol. After centrifugation, components within the density range of 1.10–1.12 g / mL are collected to obtain the Chlorella exovesicle product. This invention achieves concentrated and precise collection of Chlorella exovesicles by regulating membrane lipids during the early induction culture of Chlorella, combined with TFF concentration and density gradient centrifugation. This method effectively eliminates impurities, achieving a recovery rate of over 60% and a purity of 2.56 × 10⁻⁶. 10 The particle / μg method provides an efficient, controllable, and scalable solution for the industrial-scale separation and preparation of microalgal exovesicles.

The present application relates to a kind of chlorella-nanose complex hydrogel and its preparation method and application, belong to nanose antibacterial technical field.The chlorella-nanose complex hydrogel includes hydrogel matrix, and the chlorella and photo-thermal nanose loaded on the hydrogel matrix.The present application also provides the preparation method of the complex hydrogel, and the application in the preparation of antibacterial drug or dressing and the killing gram-negative bacteria and gram-positive bacteria.The present application combines chlorella and nanose for the first time, and constructs a self-oxygen supply antibacterial platform.The chlorella in the chlorella-nanose complex hydrogel can carry out photosynthesis under illumination, continuously produce oxygen, provide sufficient substrate for nanose oxidase-like reaction, overcome the low activity defect of traditional nanose in anoxic environment.

The application discloses a preparation method of mycelial pellet composite material for enhanced denitrification of micro-polluted water, and comprises the following steps: screening and obtaining a bacterial strain and a fungal strain, and preparing a bacterial suspension; culturing the fungal strain to prepare mycelial pellets; culturing the bacterial suspension in a mycelial pellet culture medium to prepare DMP; modifying biochar by KMnO4 to prepare modified biochar, and preparing a modified biochar suspension; adding the modified biochar suspension and a chlorella suspension into the DMP, and oscillating and culturing to prepare the mycelial pellet composite material. The mycelial pellet composite material for enhanced denitrification of micro-polluted water prepared by the application has the characteristics of structural stability, high electron transfer efficiency and strong adaptability.

This invention provides a chemical chain gasification method for Chlorella, belonging to the field of biomass energy conversion technology. The invention involves acid treatment of Chlorella to remove endogenous alkali (earth) metals and some ash, avoiding interference with the gasification reaction while retaining the organic active components of Chlorella. Calcination of ilmenite improves its mechanical strength, facilitating recycling, and converts it into an active state, enhancing its reactivity and increasing its selectivity for H2 and CO, particularly H2. This achieves efficient gasification of Chlorella, yielding high-purity syngas. Furthermore, the use of natural ilmenite reduces costs.

The present application relates to a kind of illumination culture methods for improving chlorella pyrenoidosa biomass, the illumination culture method includes the following steps: chlorella pyrenoidosa stock solution is inoculated in sterile liquid medium, and chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light.The present application develops a kind of culture method for chlorella pyrenoidosa, i.e.chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light, the culture method is simple to operate, easy to implement, the method can significantly improve the growth rate and biomass accumulation of chlorella pyrenoidosa, realize the high-quality large-scale production of chlorella pyrenoidosa;It can also reduce the production energy consumption per unit biomass, solves the technical problem that yield increase and consumption reduction are difficult to consider in microalgae culture.

The application provides a sensor with antioxidant and antifouling properties and a preparation method and application thereof, and belongs to the technical field of marine chemistry. The sensor takes a glassy carbon electrode (GC) as a substrate, adopts polyaniline as a solid contact layer, and takes a calcium ion selective membrane (ISM) as a detection core. The innovation of the sensor is that a fucoidan-based functional polypeptide (PFp) is modified on the ISM to construct an antifouling layer. The PFp layer forms a triple synergistic defense mechanism of "anti-adhesion-sterilization-antioxidation". Experiments prove that the sensor can effectively inhibit the adhesion and colonization of bacteria such as escherichia coli and staphylococcus aureus and chlorella, and significantly slow down the oxidative decay of the sensing membrane. In addition, when the sensor is used for calcium ion detection in actual seawater, it exhibits excellent long-term stability and high precision, and provides an innovative strategy for continuous in-situ monitoring in complex media.

This invention relates to the field of aquatic feed additives and aquaculture technology, specifically a method and application for improving the selenium content and meat quality of *Siniperca maculatus* using selenium-enriched algae. The steps include: screening *Chlorella* from naturally selenium-rich water bodies and the intestines of *Siniperca maculatus*; culturing *Chlorella* in BG11 medium containing 4 mg / L Na2SeO3 at 25°C under 1540 μmol / L photon illumination for one week; then scaling up the culture in a photobioreactor for four weeks; centrifuging, washing, and vacuum freeze-drying to obtain selenium-enriched algae powder; adding the powder at 0.1~0.9 mg / kg to the basic feed to make pelleted feed, and feeding it to *Siniperca maculatus* with an initial body weight of 10±2 g. This method can increase the selenium content in fish muscle by 20%~111%, reduce the content of heavy metals such as As, Cd, and Hg, improve growth performance and meat quality, and is safe, easily scalable, and contributes to the green development of aquaculture.

The present disclosure relates to a biodegradable geotextile structure for soil biostimulation comprising a woven fabric structure of hydrophilic filament yarns of a cellulose fiber selected from the list consisting of: alpha cellulose, beta cellulose, gamma cellulose or mixtures thereof; at least a plant-based biopesticide selected from the list consisting of: Nicotine, Rotenone, Pyrethrins I, Pyrethrins II, Eugenol, Citronella oil, and mixtures thereof; at least a microalgae selected from the list consisting of: Chlorella spp., Scenedesmus spp, Dunaliella spp, Spirulina spp., and mixtures thereof. Furthermore, the present disclosure relates to a method to obtain said biodegradable geotextile structure.

This invention belongs to the field of Chlorella application technology, specifically relating to a method for extracting nanocellulose from Chlorella, comprising the following steps: S1, defatting dried Chlorella powder using the methanol-chloroform method; S2, adding anhydrous acetone to the defatted Chlorella powder, then adding 2,2-dimethoxypropane and p-toluenesulfonic acid to react, and centrifuging to collect the precipitate after the reaction, obtaining protected Chlorella cellulose; S3, deproteinizing with an alkali method; S4, adding an acetic acid aqueous solution to the deproteinized protected cellulose, centrifuging to collect the precipitate after the reaction, washing the precipitate to neutral and then dialyzing to obtain deprotected Chlorella cellulose; S5, bleaching and purification; S6, preparing Chlorella nanocellulose. This invention, by adding 2,2-dimethoxypropane to defatted Chlorella powder, forms a stable protecting group, preventing cellulose degradation under strong alkali, thereby reducing the breakage and loss of cellulose molecular chains.

The application discloses a preparation method and application of chlorella exosome and mRNA composite nanoparticles, and has the characteristics that chlorella is inoculated in BG11 culture medium and cultured for 6-8 days, then inoculated in BG11 culture medium added with glucose and cultured, on the second day of the culture, exogenous stress is given or no treatment is given, finally, chlorella algae liquid is collected after culturing for 5-6 days, and chlorella exosome is obtained through differential centrifugation; the exosome is mixed with mRNA at a mass ratio of 1:1-1:3, an electroporation buffer is added, and the mixed liquid in a colorimetric dish is subjected to electroporation through an electroporation instrument, and the collected precipitated particles are the mRNA composite nanoparticles of the chlorella exosome, and the chlorella exosome has the effects of enhancing CAT and SOD in vitro and / or antagonizing MDA, in vitro antioxidant property and in vivo oxidative stress reliever of zebra fish, and can be used as a carrier to load and protect mRNA, so that targeted delivery and intracellular expression are realized.

Provided are a method for reducing the beany flavor of a textured vegetable protein containing soy protein, the method of which comprises using Chlorella, and a method for preparing a textured vegetable protein with reduced beany flavor, the method of which comprises using Chlorella.

The present application relates to the field of aquaculture breeding technology, and discloses an indoor cement pool artificial breeding method for east wind snail, which comprises the following steps: storing east wind snail egg sacs after disinfecting water body by ultraviolet rays, removing infected egg sacs, and performing specific water flow replacement 24 hours before hatching; feeding the larvae with a nutrient agent containing clostridium aceticum protein and agaricus blazei polysaccharide every day after hatching, feeding the larvae with monosomic algae such as chrysomonad, tetraselmis, chlorella, etc. in the early stage, and feeding the larvae with shelled algae in the middle stage until the larvae are attached and metamorphosed; keeping the breeding pool continuously aerated and controlling parameters such as dissolved oxygen, salinity, temperature and pH value of the water body during the breeding period, and finally collecting the snail seedlings to end the breeding. The indoor cement pool artificial breeding method for east wind snail provided by the present application is suitable for one or more of fasciolaria fasciolaris and mud east wind snail, and can effectively improve the success rate and seedling quality of indoor cement pool breeding of east wind snail.

The application discloses a 3D bioprinting bacteria-algae symbiotic engineering living material and a preparation method and application thereof. The method takes chlorella as a biological oxygen supply source, takes alkali-producing bacteria as phenol-degrading bacteria, takes sodium alginate and carboxymethyl cellulose as carriers, and respectively prepares bacterial bio-ink and algal bio-ink added with silicon dioxide, so as to build bacteria-algae symbiotic engineering living materials with stripe and layer configurations through a 3D bioprinting mechanism. The bacteria-algae symbiotic engineering living material realizes three-dimensional accurate arrangement and functional partition of bacteria and algae, and exhibits excellent degradation performance and photosynthetic performance under different phenol concentrations, and has application prospects in the biological degradation treatment of organic pollutants.

The application belongs to the technical field of skin care products, and particularly relates to a composition containing anti-wrinkle composite peptides, a preparation method and application thereof. The composition is composed of the following raw materials in mass percentage: acetyl hexapeptide-8 0.09-0.11%, chlorella extract 1.2-1.8%, arginine / lysine polypeptide 0.003-0.005%, dipeptide diaminobutyryl benzyl amide diacetate 0.09-0.11%, ginsenoside 0.20-0.25%, octisalate 0.70-0.75%, 1,2-hexanediol 0.5-1%, PEG-40 hydrogenated castor oil 1.02-1.14%, PPG-26-butanediol polyether-26 1.53-1.71%, butanediol 24-26%, and the balance being water. The performance of the composition is detected, and the results show that the composition has excellent anti-wrinkle effect.

This invention discloses a green extraction and preparation method for Chlorella protein, belonging to the field of protein processing. The method includes: pre-treatment of Chlorella protein-producing cells by ball milling to disrupt cell walls; extraction using a microwave-assisted deep eutectic solvent; separation by water extraction; and drying to obtain Chlorella protein. This method can directly produce high-purity Chlorella protein, and the resulting product possesses excellent physicochemical properties, functional characteristics, and biological activity. This invention provides solid technical support for the green and efficient extraction and application of Chlorella protein.

The application discloses an anti-flagellate predation chlorella and a screening method thereof. The method comprises the following steps: S1, inoculating chlorella and flagellates into the same culture medium at a preset number ratio; S2, when the number of chlorella decreases to a preset threshold and then starts to increase, taking the culture solution containing chlorella and coating on a selective culture medium; S3, picking the chlorella single algae from the selective culture medium, purifying through a plate streaking method or a limited dilution method, and obtaining a single clone chlorella; S4, expanding the single clone chlorella, and verifying the resistance of the expanded single clone chlorella to flagellate predation; S5, if the expanded single clone chlorella has the resistance to flagellate predation, the anti-flagellate predation chlorella is obtained; if the expanded single clone chlorella does not have the resistance to flagellate predation, repeating steps S1-S4 until the expanded single clone chlorella has the resistance to flagellate predation, and the anti-flagellate predation chlorella is obtained.

This invention discloses a yeast strain isolated from Chlorella symbiotic bacteria and its application in treating organic waste liquid from kitchen waste. Meyerozyma guilliermondii The strain SHS0923, with accession number CCTCCNO:M20252128, is deposited at the China Center for Type Culture Collection, Wuhan University, on September 26, 2025. This invention addresses the problems of existing strains exhibiting low adaptability to food waste, strong enzymatic dependence, difficulty in simultaneous purification, and low added value of the products. This strain achieves a carbon source utilization efficiency >80% in the unenzymatically hydrolyzed supernatant containing 70% hot pot food waste, reaches a biomass of 4 g / L after 4 days of shaking culture, and achieves COD, ammonia nitrogen, and total phosphorus removal rates of 71%, 81%, and 92%, respectively. It exhibits the highest intracellular LPC 18:1 abundance and a sedimentation efficiency >80% after 10 minutes.

This invention belongs to the field of microalgae protein extraction technology, and relates to a method for efficient extraction and preparation of Chlorella protein based on the eutectic solvent DES, comprising the following steps: (1) mixing Chlorella powder, DES hydrogen bond acceptor and water, performing ultrasonic-assisted extraction, centrifuging and filtering to obtain the extract; (2) adding a precipitant to the extract for precipitation; (3) purifying and freeze-drying the precipitate to obtain Chlorella protein. This invention is based on the DES system combined with ultrasonic energy to efficiently destroy the cell wall of Chlorella, promote protein dissolution, and improve the extraction rate of Chlorella protein. By selecting the DES hydrogen bond acceptor, the ratio of DES to Chlorella powder, the material-liquid ratio, and the ultrasonic intensity and time are optimized to further improve the extraction rate of Chlorella protein. Moreover, the process is mild, the method is simple to operate, the solvent is recyclable, and the environment is environmentally friendly, making it suitable for large-scale production. The extracted protein retains its natural conformation and activity, making it suitable for the development of high-value-added products.