122 results about "Chlorella sp." patented technology

The invention discloses a bacterial strain for degrading aspartame, a bacterial-algae symbiotic system and application of the bacterial strain and the bacterial-algae symbiotic system, the bacterial strain is corn sphingomonas zeae NCU J1, and the preservation number of the bacterial strain is CCTCC (China Center for Type Culture Collection) NO: M 20251462. And an algal-bacterial symbiotic system is constructed by adopting an oriented symbiotic combination of the corn sphingomonas NCU J1 and the chlorella sp. The corn sphingomonas NCU J1 can metabolize an artificial sweetening agent aspartame and degradation products thereof, metabolism complementation can be achieved through an algal-bacterial symbiotic interface, and bacteria preferentially promote aspartame to be subjected to photohydrolysis to generate degradation products such as diketopiperazine derivatives. The microalgae synchronously start isomerism enzymatic reaction to convert degradation products such as diketopiperazine derivatives into low-toxicity products, the risk of secondary pollution is reduced by 40% or above compared with that of a traditional physical and chemical method, 150 mg / L aspartame can be efficiently degraded within 2 days, generation of derivatives of aspartame can be greatly reduced, secondary pollution is reduced, and the method is suitable for industrial production. And the method has a good application prospect in the aspect of rapidly treating aspartame-containing wastewater.

The invention discloses a phycomycete symbiotic system and application of the phycomycete symbiotic system in treatment of aquaculture tail water, and belongs to the technical field of tail water treatment. The phycomycete symbiotic system comprises chlorella, scenedesmus and activated sludge, and the initial inoculation dry weight ratio of the chlorella to the scenedesmus to the activated sludge is 1: 1: 6. And exogenously adding octanoyl-L-homoserine lactone or tetradecanoyl-L-homoserine lactone into the phycomycetes symbiotic system, so as to construct the phycomycetes symbiotic system with enhanced functions. On the basis of a mixed algae-bacteria symbiotic system with an optimized ratio, directional enhancement of functions is realized by virtue of quorum sensing signal molecules, the stability and resistance gene interception capability of the quorum sensing signal molecules under the stress of antibiotics are verified, and finally efficient purification of the aquaculture tail water is realized. According to the invention, the blank of the prior art in the aspects of advanced regulation and control of a phycomycete synergistic mechanism and synergistic treatment of emerging pollutants is filled up.

The invention belongs to the technical field of microorganisms, and discloses chlorella sorokiniana M6, application of the chlorella sorokiniana M6 and a method for synchronously synthesizing xanthophyll and protein. The preservation number of the Chlorella sorokiniana M6 provided by the invention is GDMCC NO.66205, the Chlorella sorokiniana M6 has an excellent capability of co-producing lutein and protein, and is almost free of chlorophyll synthesis, so that the Chlorella sorokiniana M6 has very important economic value and practical significance for realizing commercial application of microalgae protein and lutein production.

The invention provides a method for regulating and controlling growth and metabolism of caproic acid bacteria by using marine active peptides in a baijiu fermentation process. According to the method, the marine active peptides are added in an anaerobic fermentation stage of baijiu preparation to regulate and control the growth and metabolism of the caproic acid bacteria; wherein the marine active peptide is chlorella pyrenoidosa peptide, schizochytrium limacinum peptide, fish albumin peptide or euphausia superba peptide. According to the method, active peptides with specific sources are added into an anaerobic culture system, so that the growth of caproic acid bacteria can be promoted, energy metabolism can be enhanced, and the activity of key enzymes such as caproic acid dehydrogenase and caproic acid synthase can be activated, and therefore, the generation amount of flavor acids such as caproic acid and butyric acid can be increased, the fermentation quality can be improved, and the flavor level and esterification precursor substance content of Baijiu can be improved.

This invention belongs to the field of functional food processing technology, specifically relating to a method for improving the flavor of a chlorophyll-deficient Chlorella proteoglycan mutant. The method includes the following steps: S1, obtaining a Chlorella proteoglycan mutant strain; S2, centrifuging the Chlorella proteoglycan mutant strain and collecting the algal sludge, then pulverizing the collected algal sludge and dispersing it in water to obtain an algal powder suspension, then adding a compound enzyme to the algal powder suspension, and inactivating the enzyme after the reaction; S3, cooling the system to room temperature, adding glucose and stirring to dissolve, sterilizing, inoculating with a compound bacterial strain and fermenting, and sterilizing again after fermentation; S4, cooling the fermented system to room temperature, then adding β-cyclodextrin and stirring, and drying the system after stirring to obtain the algal protein product. This invention employs a synergistic process of compound enzymatic hydrolysis, compound bacterial fermentation, and β-cyclodextrin encapsulation to remove the off-flavor of the mutant algae from the source, significantly improving the flavor and enhancing the product's storage stability.

The invention provides a biological bait culture method for pond culture water. The biological bait culture method comprises the following steps: S1, building a three-dimensional culture system; s2, preparing an ecological base and a biocenosis; according to the method, by building a three-dimensional cultivation system of the bottom layer modular net cage, the water surface floating type three-dimensional cultivation frame and the intelligent regulation and control system and combining scientific configuration of ecological bases and biocenosis, the problem that bait supply is unstable in traditional cultivation is effectively solved; the light organism cultivation unit, the plant planting unit and the biological membrane carrier unit of the water surface floating type three-dimensional cultivation frame work in a layered mode, bait organisms such as chlorella, copepods and rotifers in different water layers are cultivated in a directional mode, a complete closed-loop food chain is constructed in addition to food filtering and nutrition supplementing of snails and freshwater mussels, and the bait yield per unit water body is greatly increased.

The invention discloses a decolorized chlorella protein, a preparation method thereof and application of the decolorized chlorella protein in preparation of foaming liquid, and relates to the technical field of biology. The preparation method comprises the following steps: extracting and decolorizing chlorella protein of chlorella pyrenoidosa, and drying to obtain the decolorized chlorella protein. According to the invention, an efficient chlorella protein extraction technology suitable for industrial production is established; the chlorella protein is treated by adopting an ethanol decolorizing method, the influence of two decolorizing methods and different decolorizing times on the decolorizing effect, the protein structure and the physicochemical property is systematically evaluated, the decolorized chlorella protein foaming liquid is prepared and represented, and the application effect of the decolorized chlorella protein foaming liquid in the foamed coffee is verified. Results show that the decolorized chlorella protein provided by the invention has excellent foaming characteristic, has the advantages of high protein purity and high sensory acceptance, and provides important technical support for efficient extraction of chlorella protein and widening of application range of chlorella.

The invention discloses chlorella capable of resisting acetate and urea and a breeding method and application thereof, and relates to the technical field of microorganisms, the chlorella is a Sorokiniana mutant strain, the classification name of the chlorella is Chlorella sorokiniana-TongWei-01, the chlorella is preserved in the China General Microbiological Culture Collection Center at 2025-12-1, and the preservation number of the chlorella is CGMCC NO. The address of the preservation unit is Institute of Microbiology, Chinese Academy of Sciences, No.3, No.1 Yard, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.46630. According to the method, large-scale culture can be performed by taking acetic acid / sodium acetate as a unique carbon source and urea as a unique nitrogen source, the method has excellent acetic acid utilization efficiency and urea tolerance, toxicity interference is resisted while two substrates are efficiently converted, high-density culture supported by a cheap carbon-nitrogen source is realized, and a core algae species support is provided for large-scale production of microalgae.

The present invention relates to the compositions comprising algae, specifically Spirulina platensis and Chlorella, and their use for the treatment of crops, such as fruits. vegetables and arable crops against heat stress, especially sunburn.

The invention provides a skin repairing composition for relieving red, itching and dry symptoms of sensitive skin and application, and belongs to the technical field of cosmetics. The composition comprises an effective amount of an extract of at least two algae selected from the group consisting of Phaeodactylum tricornutum, Spirulina, Chlorella and Micrococcus, and optionally comprises other skin repair ingredients cooperating therewith. The skin repair composition for relieving red, itching and dry symptoms of sensitive skin simultaneously and synergistically intervenes four major causes of sensitive skin formation, namely barrier damage, hyperneurosis, immune inflammation and micro-ecological imbalance, breaks vicious circle and establishes a repair closed loop by utilizing the multiple effects of specific algae active ingredients, and has the effects of relieving the symptoms of red, itching and dry symptoms of sensitive skin and relieving the symptoms of red, itching and dry symptoms of sensitive skin. Therefore, the problems of red skin, itching skin, dry skin and the like are radically relieved.

This invention relates to a method for immobilizing Chlorella, in order to... Chlorella Using *Chlorella sp. FACHB-9* as the culture medium, *Chlorella* solution was inoculated into the culture medium and cultured under conditions where light intensity varied with concentration to obtain a high-concentration *Chlorella* solution. Chitosan-modified rope-shaped artificial aquatic plants, used as an immobilization carrier, were added to the high-concentration *Chlorella* solution and fixed in a constant-temperature shaker at 25–35℃ and 120–180 rpm for 20–30 hours. After removal and drainage, immobilized *Chlorella* was obtained. An immobilized *Chlorella* was prepared by the above method. An application of the immobilized *Chlorella* was also described: the immobilized *Chlorella* was used to remove nitrogen and phosphorus from aquaculture wastewater. The beneficial effects included: effective removal of nitrogen and phosphorus pollution from aquaculture wastewater, prevention of eutrophication, reduction of wastewater treatment costs, inhibition of *Microcystis aeruginosa* growth, and creation of additional economic value through the resource utilization of *Chlorella*.

The application discloses a kind of preparation methods of bacteria-algae symbiosis filler, specifically: to the reactor filled with filler, add active sludge dispersion liquid, under aeration condition, 7-10 days are operated, and simulate tail water is passed through during operation process;Then inoculate chlorella according to the volume ratio of active sludge dispersion liquid and chlorella dispersion liquid 1:5-50, 22-30 DEG C and under illumination, 14-20 days are operated, wherein, light dark ratio is: illumination 6-12h, dark 12-18h;Simulate sewage plant tail water is passed through during operation process;Get the filler that hangs bacteria-algae symbiosis biofilm.The bacteria-algae symbiosis film formed in the application, microalgae can promote the enrichment of denitrifying bacteria and organic matter metabolic functional bacteria, and bacteria can improve the photosynthesis performance and carbon fixation performance of microalgae through material exchange and information transmission, so that the bacteria-algae symbiotic system can efficiently purify N / P / organic matter in water body, while reducing greenhouse gas emissions.The application also discloses a filler combination structure based on the above bacteria-algae symbiotic filler.

The invention discloses an application of a transcription factor ChZF7 of a basophilic microalgae Chlorella sp. BLD in regulation and control of salt tolerance of microalgae. According to the invention, the coding gene of the transcription factor ChZF7 is transferred into chlamydomonas reinhardtii by using a transgenic technology after intron insertion and codon optimization, so that transgenic chlamydomonas reinhardtii is obtained. The salt tolerance of the chlamydomonas reinhardtii with the over-expressed ChZF7 gene is remarkably improved, the accumulation of neutral fat and active oxygen under the stress of 225mM salt is less than that of the chlamydomonas reinhardtii in a control group, the content of penetrating substance proline is increased, in addition, the change of cell components in the chlamydomonas reinhardtii under the stress of salt can be weakened by the over-expressed ChZF7 gene, and the salt tolerance of the chlamydomonas reinhardtii is improved. The transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 can regulate and control the salt tolerance of the chlamydomonas reinhardtii, a foundation is laid for genetic improvement and directed molecular breeding of the microalgae, the transcription factor ChZF7 plays an important role in improvement of the salt tolerance of the microalgae, reutilization of salinized water resources is facilitated, and the transcription factor ChZF7 and the gene coded by the transcription factor ChZF7 have wide application prospects.

The invention discloses an anti-oxidation and anti-aging composition and application thereof.The anti-oxidation and anti-aging composition comprises a chlorella extract and a rice chaff extract, practice shows that after the chlorella extract and the rice chaff extract are combined, the anti-oxidation and anti-aging effect can be achieved, and the obvious amplification can be achieved in an anti-oxidation test-DPPH free radical scavenging experiment; and after combination, no negative influence is caused on the activity of the fibroblast, and the expression level of the COL I of the fibroblast is obviously improved. In addition, an obvious effect can be achieved under the condition of low concentration, large-amount addition is not needed, and the effect is stable.

This invention discloses a method for isolating and preparing Chlorella exovesicles. Specifically, Chlorella, conventionally cultured to the early logarithmic growth phase, is induced to develop in a nitrogen-limited medium. The culture medium is then concentrated using a tangential flow filtration system. Finally, the concentrate is subjected to density gradient centrifugation with iodixanol. After centrifugation, components within the density range of 1.10–1.12 g / mL are collected to obtain the Chlorella exovesicle product. This invention achieves concentrated and precise collection of Chlorella exovesicles by regulating membrane lipids during the early induction culture of Chlorella, combined with TFF concentration and density gradient centrifugation. This method effectively eliminates impurities, achieving a recovery rate of over 60% and a purity of 2.56 × 10⁻⁶. 10 The particle / μg method provides an efficient, controllable, and scalable solution for the industrial-scale separation and preparation of microalgal exovesicles.

The utility model relates to a park landscape system based on ecological interaction type of chlorella micro-aquatic ecosystem, it is to solve the technical problem of the poor interactivity function of existing intelligent park, the park landscape system of the utility model includes pool, dot matrix light source, energy storage battery, motion power generation facility, solar panel, photosynthesis monitor, controller, the small chlorella is put in the pool, the dot matrix light source is laid in the pool bottom, and the solar panel, motion power generation facility is connected with energy storage battery respectively, and the energy storage battery is connected with the dot matrix light source, and the photosynthesis monitor is set in the pool top, and the small chlorella growth state in the pool is monitored and is sent to the controller, and the controller is also connected with the dot matrix light source, controls the light mode and the light intensity of each light point in the dot matrix light source. Participants carry out the claim to the small chlorella in the interactive pool through the interactive applet on the controller of mobile phone scanning controller, and send control command, participate in the landscape formation in the pool, can be used in the field of city park.

The invention belongs to the technical field of soil remediation, and relates to a microalgae-microorganism compound preparation for improving saline-alkali soil and application. The microalgae-microorganism compound preparation is prepared from spirulina, chlorella, nostoc, scenedesmus obliquus, bacillus mucilaginosus, bacillus mucilaginosus and pseudomonas. The microalgae-microorganism compound preparation for improving the saline-alkali soil can improve the available phosphorus content of the soil, increase the available potassium content of the soil and effectively improve the soil; soil bulk density is reduced, and soil hardening is improved; therefore, the fertilizer retention property of the soil is enhanced, the growth and development of crop roots are facilitated, the crop yield is remarkably improved, and the application prospect is wide.

The invention belongs to the technical field of antibacterial hydrogel preparation, and particularly relates to antioxidant antibacterial hydrogel as well as a preparation method and application thereof. The method comprises the following steps: dispersing chitosan in water, then adding glacial acetic acid for reaction, adding 2, 3-epoxypropyltrimethylammonium chloride for continuous reaction after the reaction is finished, centrifuging after the reaction is finished to obtain supernate, and then adding a mixed solution of acetone and ethanol for precipitation, suction filtration and freeze drying to obtain chitosan quaternary ammonium salt; the preparation method comprises the following steps: dissolving chitosan quaternary ammonium salt in water, adding Pluronic F-127, stirring until the Pluronic F-127 is completely dissolved, adding the freeze-dried chlorella powder, and uniformly stirring to obtain the antioxidant and antibacterial hydrogel. The characteristics of the wound microenvironment are improved through the freeze-dried chlorella, and the freeze-dried chlorella is cooperated with the antibacterial hydrogel in function, so that an efficient treatment effect is achieved; meanwhile, the preparation process is simple, the raw material source is wide, and the clinical application prospect is good.

The present application relates to a kind of chlorella-nanose complex hydrogel and its preparation method and application, belong to nanose antibacterial technical field.The chlorella-nanose complex hydrogel includes hydrogel matrix, and the chlorella and photo-thermal nanose loaded on the hydrogel matrix.The present application also provides the preparation method of the complex hydrogel, and the application in the preparation of antibacterial drug or dressing and the killing gram-negative bacteria and gram-positive bacteria.The present application combines chlorella and nanose for the first time, and constructs a self-oxygen supply antibacterial platform.The chlorella in the chlorella-nanose complex hydrogel can carry out photosynthesis under illumination, continuously produce oxygen, provide sufficient substrate for nanose oxidase-like reaction, overcome the low activity defect of traditional nanose in anoxic environment.

The present application relates to the technical field of aquatic environment, and in particular to a lactic acid bacteria, a separation and screening method and application, wherein the lactic acid bacteria is Lacticaseibacillus casei FC-5 with a preservation number of CCTCC NO: M 2026260, and the present application further provides a method for application of the lactic acid bacteria and chlorella in bio-floc technology, and solves the problem of serious threat of accumulation of nitrite nitrogen to aquatic animals in aquatic environment, and also solves the problem of serious threat of accumulation of ammonium nitrogen to aquatic animals.

The application discloses a preparation method of mycelial pellet composite material for enhanced denitrification of micro-polluted water, and comprises the following steps: screening and obtaining a bacterial strain and a fungal strain, and preparing a bacterial suspension; culturing the fungal strain to prepare mycelial pellets; culturing the bacterial suspension in a mycelial pellet culture medium to prepare DMP; modifying biochar by KMnO4 to prepare modified biochar, and preparing a modified biochar suspension; adding the modified biochar suspension and a chlorella suspension into the DMP, and oscillating and culturing to prepare the mycelial pellet composite material. The mycelial pellet composite material for enhanced denitrification of micro-polluted water prepared by the application has the characteristics of structural stability, high electron transfer efficiency and strong adaptability.

The invention relates to the technical field of biological fermentation, and discloses a production method and application of a chlorella yeast fermentation filtrate rich in exosome vesicles, and the production method comprises the following steps: chlorella culture, yeast culture, mixed fermentation and fermentation broth filtration. Wherein a culture medium containing a nitrogen source, a phosphorus source, a magnesium salt, a calcium salt, an iron salt, vitamins and the like is adopted for chlorella culture, inoculation is performed according to the volume ratio of 30%, and culture is performed at proper temperature and illumination; saccharomyces cerevisiae culture comprises activation, liquid culture medium preparation and liquid fermentation which are carried out under a sterilization condition, and shake culture is carried out at a proper temperature and a proper rotating speed after inoculation; the mixed fermentation comprises the following steps: mixing chlorella liquid with yeast liquid, supplementing a small amount of cane sugar, shaking, standing and culturing; and performing coarse filtration on the fermentation finishing solution by using filter paper and performing multi-stage filter membrane filtration to obtain a sterile filtrate rich in the chlorella saccharomycetes exosome. The production method disclosed by the invention is simple in culture condition, low in equipment requirement, low in production energy consumption and short in production period.

This invention discloses a Chlorella heptapeptide TGPLGSR with multi-target lipid-lowering activity, its preparation method, and its applications. The amino acid sequence of this heptapeptide is Thr-Gly-Pro-Leu-Gly-Ser-Arg. The preparation method includes: performing ultraviolet and electroporation combined mutagenesis on wild-type Chlorella proteoglycans to obtain a high-protein-content mutant algal strain; obtaining algal protein through fermentation and synergistic extraction and purification with alcohols; directionally digesting the protein with alkaline protease, followed by enriching the active peptide components through ultrafiltration and gel chromatography; and finally, screening the heptapeptide using liquid chromatography-mass spectrometry (LC-MS), computer-aided prediction, and molecular docking techniques. This heptapeptide exhibits potent inhibitory activity against pancreatic lipase and can dose-dependently reduce the accumulation of triglycerides and cholesterol in 3T3-L1 cells. Its mechanism involves multiple target pathways, including inhibition of MMP-9, activation of AMPK phosphorylation, and downregulation of PNLIP, and it can be used to develop lipid-lowering functional foods or health products.

The invention provides a method for removing protozoa in a chlorella species conservation bottle, which adopts an algae culture tool and seawater to disinfect, and is characterized in that: sodium hypochlorite, bleaching powder or bleaching powder concentrate and other disinfectants containing available chlorine are added to ensure that the concentration of available chlorine in algae reaches 5mg / L. Then, standing the water body for 1 hour so as to settle protozoa to the water bottom; separating the algae liquid on the upper layer into a new container, adding sodium thiosulfate with the addition amount being 1 / 10 of the available chlorine amount in the algae liquid, and neutralizing the residual available chlorine by using the sodium thiosulfate according to the concentration of 0.5 mg / L; after 48 hours of primary treatment, adding sodium hypochlorite, bleaching powder or bleaching powder concentrate and other disinfectants containing available chlorine again for disinfection, continuing to separate the algae liquid, and adding sodium thiosulfate with corresponding concentration again for neutralization; and the protozoa in the breed conservation bottle can be effectively removed after the second treatment.

The invention discloses a preparation method and application of an algae cell extract doped chitosan active composite membrane. Specifically, chitosan is adopted as a matrix, and two kinds of algal oil are introduced to enhance the functional performance of the membrane: one kind is that chlorella oil is directly doped into a chitosan solution, and the membrane is formed through a solution casting method, so that the mechanical performance and the antioxidant activity of the membrane can be effectively improved; and the other method comprises the following steps: combining schizochytrium limacinum oil with soybean protein isolate, preparing a stable Pickering emulsion by adopting an ultrasonic-assisted method, then mixing the stable Pickering emulsion with a chitosan matrix, and remarkably improving the homogeneity, the mechanical property and the functional stability of the membrane after the membrane is formed. The film material has good film-forming property, biodegradability and wide food fresh-keeping application potential.

This invention provides a chemical chain gasification method for Chlorella, belonging to the field of biomass energy conversion technology. The invention involves acid treatment of Chlorella to remove endogenous alkali (earth) metals and some ash, avoiding interference with the gasification reaction while retaining the organic active components of Chlorella. Calcination of ilmenite improves its mechanical strength, facilitating recycling, and converts it into an active state, enhancing its reactivity and increasing its selectivity for H2 and CO, particularly H2. This achieves efficient gasification of Chlorella, yielding high-purity syngas. Furthermore, the use of natural ilmenite reduces costs.

The invention discloses a photovoltaic pond river crab culture method, which comprises the following steps: a bacteria-algae compound conditioning agent is put into a target photovoltaic pond according to a preset period and a preset putting dosage, the bacteria-algae compound conditioning agent comprises an effective microbial flora microbial agent and a chlorella complex which are mixed according to a preset proportion, the pH value of the effective microbial flora microbial agent is 3.0-4.0, the pH value of the chlorella complex is 3.0-4.0, and the pH value of the chlorella complex is 3.0-4.0. The content of lactic acid bacteria in the effective microbial agent is 1.0 * 10 to 1.0 * 10 CFU / mL, the content of saccharomycetes in the effective microbial agent is 1.0 * 10 to 1.0 * 10 CFU / mL, the content of photosynthetic bacteria in the effective microbial agent is 1.0 * 10 to 2.0 * 10 < 3 > CFU / mL, the content of actinomycetes in the effective microbial agent is 1.0 * 10 < 3 > to 3.0 * 10 < 3 > CFU / mL, and the concentration of chlorella in the chlorella complex is 1.0 * 10 cells / mL; and monitoring and dynamically adjusting the water quality in the target photovoltaic pond, and adjusting the putting frequency and the putting dosage of the bacteria-algae compound regulator within a preset range according to a monitoring result so as to maintain or restore the ecological balance of the water body and the river crabs. According to the technical scheme, the problem of micro-ecological unbalance of photovoltaic shading is solved, oxygen is supplied by algae, diseases are inhibited by bacteria, and carbon sequestration by bacteria and algae is realized, so that the breeding survival rate is increased, and the growth of river crabs is promoted.

The invention provides a composite microalgae preparation constructed for biological crust as well as a preparation method and application of the composite microalgae preparation, and relates to the technical field of biological soil crust repair. The composite microalgae preparation comprises chlorella with the number of FACHB-2900, chlamydomonas with the number of FACHB-1935, a sodium alginate aqueous solution and a calcium lignosulphonate aqueous solution, wherein the chlorella is purchased from a freshwater algae seed bank of Chinese Academy of Sciences. After the composite microalgae preparation is applied, the components of the sodium alginate and the calcium lignosulphonate immediately form a layer of viscous and hygroscopic liquid mulching film on the surface layer of soil, and the mulching film can be used for bonding soil particles, reducing raindrop impact and keeping moisture. In the protective layer, the fixed microalgae are activated by environmental moisture and illumination to restore activity, start to grow and perform photosynthesis, and quickly convert into a living and self-sustaining biological soil crust, so as to quickly protect and repair exposed soil.

The invention discloses a broiler feed additive containing chlorella and application of the broiler feed additive. The additive contains chlorella; the feed also contains one or more of lactobacillus johnsonii, enterococcus faecalis, saccharomyces cerevisiae and bacillus amyloliquefaciens. By adding the additive disclosed by the invention into daily ration, the intestinal tissue form can be improved by regulating antioxidant and immune functions, so that the growth performance of the broiler chicken can be remarkably improved. In the additive, lactobacillus johnsonii BS15 and enterococcus faecalis YP-2 are combined for use, and saccharomyces cerevisiae WHY-7 and bacillus amyloliquefaciens TL are combined for use, so that the average weight of the broiler chicken is increased, the spleen index after LPS stimulation is reduced, and synergistic interaction is achieved.

The present application relates to a kind of illumination culture methods for improving chlorella pyrenoidosa biomass, the illumination culture method includes the following steps: chlorella pyrenoidosa stock solution is inoculated in sterile liquid medium, and chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light.The present application develops a kind of culture method for chlorella pyrenoidosa, i.e.chlorella pyrenoidosa is illuminated using the composite spectrum of white light and red light, the culture method is simple to operate, easy to implement, the method can significantly improve the growth rate and biomass accumulation of chlorella pyrenoidosa, realize the high-quality large-scale production of chlorella pyrenoidosa;It can also reduce the production energy consumption per unit biomass, solves the technical problem that yield increase and consumption reduction are difficult to consider in microalgae culture.