The invention relates to an experimental device for generating over-saturated general dissolved gas by high-speed jetting air doping, comprising a water column, a flowmeter, an air compressor, a general dissolved gas tester, a first valve, a second valve, a third valve, a water pump, a water flow nozzle and a gas nozzle, wherein the air compressor is connected with the flowmeter connected with the gas nozzle through the second valve, and the gas nozzle is arranged in the water column; one end of the water pump is connected with the bottom of the water column, the other end of the water pump is connected with the water flow nozzle through the first valve, and the water flow nozzle is arranged in the water column; the third valve is installed at the bottom of the water column; and the general dissolved gas tester is inserted to the water column when testing. The experimental device can realize the control on the measurement of main physical conditions affecting the generation of the over-saturated general dissolved gas, such as the jetting flow speed of a water body, air-doped amount, pressure, water depth and the like, thereby achieving the purpose of quantitatively researching theair-doped jetting flow of the over-saturated general dissolved gas; and the experimental device has the important promoting function on protecting the aquatic ecosystem of rivers and establishing environmental-friendly hydraulic engineering.