The invention discloses an urban inland river water quantity and water quality optimization regulation and control method. The method comprises the following steps: 1, carrying out current situation investigation on rainfall and evaporation quantity of an area, a pollution source of the area, hydraulic engineering of the area, river basic information, a river water environment, a river ecosystem and the like; 2, analyzing current situations such as regional pollution sources, river channel water quality and quantity, regional water environment capacity, river channel ecological water demand, regional fresh water availability, water regulation quantity and influence range, feasibility of water reuse in an intra-regional sewage treatment plant, regional water ecology and the like; and 3, providing a water quantity and water quality optimization regulation and control method. The method is based on deep analysis of the hydrological water environment in a city; an urban inland river waterquantity and quality optimization regulation and control scheme can be put forward in a targeted mode, and therefore the problems that the water body river network water quality is generally poor, thenitrogen and phosphorus content is high, the transparency is poor, the water supplementing source is single, a water ecosystem is imperfect, and the water body self-purification capacity is low in the urban inland river are solved.