The invention relates to the technical field of
environmental monitoring and ecological
environmental protection, and discloses a
reclaimed water river
ecological health evaluation method based on
environmental DNA and a co-occurrence network, and the method comprises the steps: setting sampling points, and collecting a
water sample of a monitoring region; extracting environment
DNA (Deoxyribonucleic Acid); carrying out PCR (
Polymerase Chain Reaction) amplification and high-
throughput sequencing, and carrying out species
annotation on the three biological groups; calculating a
water quality comprehensive
pollution index, and determining a reference point; constructing a co-occurrence network of various group organisms in each
point location and calculating
network topology parameters; establishing candidate index libraries of four dimensions of
community structures, diversity, functional groups and
network topology parameters for the three biological groups; calculating the integrity index of each class group after optimization and the multi-species integrity index after optimization; and delimiting the
river health level according to the
score of the integrity
evaluation system. Therefore, the topological parameters of the ecological co-occurrence networks of various groups are included in integrity index calculation, objective weights are determined through
machine learning model training, and the
structure and function states of a
river ecosystem can be scientifically and comprehensively reflected.