15 results about "Planktonic bacteria" patented technology

The invention discloses a gallium metal organic framework material which is prepared by the following steps: (1) dissolving 2-aminoterephthalic acid and itaconic acid in an organic solvent to obtain a solution A; (2) dissolving gallium nitrate hydrate in an organic solvent to obtain a solution B containing gallium ions; and (3) mixing the solution A with the solution B, heating at 50-70 DEG C for 3-5 hours, centrifuging, collecting the precipitate, and purifying to obtain the product. Itaconic acid and 2-aminoterephthalic acid are combined as double ligands, gallium ions are used as metal nodes, the gallium metal organic framework material is jointly constructed, the technical problems that itaconic acid in a free form is short in biological half-life period and low in cellular uptake efficiency are solved, the itaconic acid and the gallium ions are coordinated to achieve anti-inflammatory and antibacterial effects, and the gallium metal organic framework material has good application prospects. The compound can remove planktonic bacteria, has strong anti-biofilm activity, and can be used for preparing drugs for treating suppurative arthritis.

The utility model relates to the technical field of planktonic bacteria, and discloses a split planktonic bacteria sampler which comprises a base, a mounting plate is mounted on the upper portion of the base, a support is movably sleeved with the side face of the mounting plate, and a limiting plate for limiting the position of the support is mounted on the upper portion of the mounting plate. The top end of the support is movably provided with a detection device main body, the interior of the detection device main body is fixedly connected with a transverse plate, and the exterior of the upper end of the transverse plate is provided with eight groups of sliding rods at equal intervals. According to the split type planktonic bacteria sampler, the position of the detection device main body is driven to be limited through the bracket, and the bracket can be mounted at the upper part of the mounting plate and is fixed through the limiting plate, or the bracket can be freely moved to different areas after being detached from the outside of the mounting plate, so that in the planktonic bacteria collecting process, the planktonic bacteria can be conveniently collected; the main body of the detection device is designed in a split manner and can be moved to a narrow area to collect the floating bacteria, so that the floating bacteria collection efficiency and convenience are improved.

The utility model belongs to the technical field of biological evaluation of medical instruments, and particularly relates to a wound biological membrane culture system and a dynamic in-vitro wound model for antibacterial activity evaluation, which comprise a culture medium dripping system, a reactor and a waste liquid collecting bottle which are sequentially connected, according to the utility model, the glass slide and the in-vitro pigskin tissue or the glass slide and the in-vitro pigskin tissue, the test dressing and the balancing weight are sequentially arranged in the test bin of the reactor from bottom to top. The in-vitro pigskin tissue is used for wound surface construction and microbial inoculation, continuous nutrition supply is provided, the in-vitro pigskin tissue in-vitro biological membrane preparation device can be used for preparing a wound surface biological membrane, planktonic bacteria infection or biological membrane infection of the wound surface can be simulated, the in-vitro pigskin tissue in-vitro biological membrane preparation device is closer to the actual clinical condition, and the antibacterial activity of the antibacterial wound surface dressing can be predicted more accurately.

The present application relates to a kind of river planktonic bacteria community composition prediction method based on artificial neural network auto-encoder, belong to environmental science field.The method is by collecting river planktonic bacteria OTU relative abundance and related water environment, meteorological data etc., form original data set, and carry out pre-processing.Using auto-encoder model, the potential space based on OTU composition and the potential space based on environmental elements are constructed by training set, the difference between the two potential spaces is minimized during training, and OTU composition is obtained from potential space by the same decoder.Using SHAP value to explain the model, understand how each environmental factor affects the model prediction of a single sample, and understand the overall impact of environmental factors on model prediction by averaging the SHAP values of multiple samples.The present application explores the change relationship between various environmental factors and river planktonic bacteria relative abundance from the perspective of data-driven deep learning model.

Compositions comprising 2-hydroxycarboxylic acid for dysregulating the metabolism of planktonic bacteria and their use. This disclosure provides compositions for dysregulating the metabolism of planktonic bacteria, the compositions comprising preferred enantiomers of 2-hydroxycarboxylic acid. This disclosure further provides compositions for dysregulating the metabolism of planktonic bacteria, the compositions comprising preferred enantiomers of 2-hydroxycarboxylic acid and antimicrobial compounds. Dysregulation of the metabolism of planktonic bacteria can be measured, among other examples, by a decrease in the MIC of concomitant antimicrobial agents, an enhancement of in vivo antimicrobial effects, or aerobic respiration of metabolically active cells (reduction of non-fluorescent resazurin to highly fluorescent resorphine), which can lead to sensitization of planktonic bacteria to antimicrobial compounds and / or disruption of resistance of planktonic bacteria to antimicrobial compounds.

The utility model discloses an automatic tester for planktonic bacteria, relates to the technical field of the automatic tester for planktonic bacteria, and provides the following scheme: the automatic tester for planktonic bacteria comprises a base, a damping bearing is mounted at the top of the base, a mounting disc is mounted on the outer side of the damping bearing, and an adjusting block is mounted on the outer side of the mounting disc; a rotary knob is fixed to the middle of the adjusting block, a clamping mechanism is annularly arranged on the periphery of the mounting disc, a mounting base is mounted on the front side of the mounting disc, a testing box is fixed to the front side of the mounting base, magnetic attraction pieces are symmetrically fixed to the two sides of the base, and connecting bolts penetrate through the middles of the magnetic attraction pieces. The magnetic suction sheet is fixed on the outer side of the base through a connecting bolt; by arranging the clamping mechanism, the mounting disc and the mounting base are stably connected to be clamped and fixed, no tool is needed for disassembly, equipment is small and flexible, disassembly and assembly are convenient and fast, it is guaranteed that the device can be disassembled, assembled and moved according to needs when floating bacteria are detected, meanwhile, disassembly and overhaul can be conveniently conducted, and maintenance operation is facilitated.

The utility model provides an air planktonic bacteria sampler which comprises a main machine and a plurality of sampling seats, the main machine and the plurality of sampling seats are respectively provided with an annular table with an extraction opening, an inner cavity of the main machine is provided with a fan communicated with the extraction opening, and the annular tables are detachably provided with trays for placing culture dishes. Each sampling seat is provided with a communicating pipe communicated with the corresponding extraction opening, the annular table is detachably sleeved with an end cover with a plurality of micropores, the annular table located on the main machine is detachably sleeved with a collecting cover with a collecting pipe, and the collecting cover is provided with a communicating assembly used for communicating the communicating pipes; after a fan drives air to vibrate through micropores in an end cover, particles fall into a culture dish to be sampled, the end cover on a main machine is replaced by a collecting cover, and the collecting cover is communicated with a plurality of communicating pipes through a communicating assembly, so that sampling work at multiple positions can be carried out at the same time, the sampling efficiency is improved, sampling can be carried out in multiple narrow spaces, and the sampling efficiency is improved. Multiple sampling working modes are provided, and multiple sampling requirements are met.

The invention belongs to the technical field of nano-drugs, and particularly relates to multifunctional honokiol nano-particles and a preparation method and application thereof.The honokiol nano-particles are prepared by using rhamnolipid modified chitosan as a carrier and loading honokiol; according to the nano-particles, an amido bond formed between rhamnolipid and chitosan can be subjected to targeted decomposition under the acidic condition of a bacterial infection part, and the released rhamnolipid can destroy a bacterial biofilm; further, permeation of the natural antibacterial agent honokiol and chitosan at the infected part is enhanced, and synergistic sterilization is realized; meanwhile, the planktonic bacteria re-adhesion resistance characteristic of rhamnolipid can inhibit the re-formation of a biofilm, the full-stage sterilization of'destroying the biofilm, killing bacteria in the film and inhibiting the re-adhesion of residual bacteria 'of the nanoparticles is finally realized, and the multifunctional honokiol nano platform is suitable for preventing and treating infectious diseases such as wound infection and bacterial peritonitis.

The embodiment of the utility model relates to the technical field of refrigeration, in particular to a refrigerator. According to the ion generator disclosed by the embodiment of the invention, the plasma is generated by arranging the opposite electrode group, so that the effect of sterilizing the refrigerating chamber can be achieved, and the plasma can also decompose peculiar smell molecules to achieve the purification effect. The third electrode is arranged outside the area of the opposite electrode set and used for generating the air negative ions, the air negative ions adsorb the planktonic bacteria in the refrigerating chamber, and the effect of removing the planktonic bacteria is achieved. According to the ion generator, the plasma can be generated, the air negative ions can also be generated, the plasma and the air negative ions are complementary, and the sterilization and odor removal effects on the refrigerating chamber are improved.

The utility model relates to the field of planktonic bacteria samplers, and discloses a flow calibrating device of a planktonic bacteria sampler, which comprises an electronic flowmeter and a connecting mechanism, one side of the front end of the electronic flowmeter is fixedly provided with a placing plate, the connecting mechanism comprises a mounting block and a fixing pipe, the middle position of the upper end of the mounting block is rotatably provided with a rotating column, and the rotating column is fixedly connected with the electronic flowmeter. A telescopic pipe is fixedly arranged at the front end of the fixed pipe, a first connecting pipe is fixedly arranged at the front end of the telescopic pipe, a connecting seat is fixedly arranged at the lower end of the first connecting pipe, a bearing is rotationally arranged at the position, close to the telescopic pipe, of the outer side of the fixed pipe, and a movable pipe is fixedly arranged at the inner end of the bearing. According to the utility model, when a plurality of planktonic bacteria samplers need to be measured in sequence, the movable pipe can be rotated to connect the flange with the exhaust hole of another planktonic bacteria sampler, the valve of the fixed pipe is closed, and the valve of the movable pipe is opened, so that the time of repeated measurement is shortened, and continuous flow measurement by a user is facilitated.

The utility model discloses a planktonic bacteria sampler flow regulating device which comprises a base, a regulating tank and a buffer tank are respectively arranged at the top of the base close to two ends, one end of the regulating tank and one end of the buffer tank are respectively provided with a mounting joint, the other end of the regulating tank is connected with the other end of the buffer tank through a connecting pipe, and a rotor flow meter is arranged at the top of the connecting pipe. A control mechanism facilitating flow adjustment is arranged on the inner side of the adjusting tank, and one-way valves are installed at the positions, close to the two ends, of the buffer tank. Gas flow in the adjusting tank can be adjusted through the arranged control mechanism, and the gas flow can be monitored in real time through the arranged rotor flow meter; and the buffer tank and the one-way valve in the buffer tank play roles in stabilizing the gas flow and preventing the gas from flowing back, so that through the structure, the precise and stable control on the gas flow of the planktonic bacteria sampler is realized, and the accuracy and reliability of sampling data are ensured.

The utility model discloses a clean room environment monitoring system, this system includes the LIMS system of installation on industrial server, environmental monitoring instrument, data acquisition module, data concentrator and controlled equipment, environmental monitoring instrument includes the dust particle sampling counter and planktonic bacteria sampling counter through pipeline and the connection of air extraction system, the LIMS system has integrated experiment reservation and data analysis function module, is used to receive environmental data and carries out analysis and judgment, and then generates instruction control controlled equipment, industrial server carries out data interaction with data concentrator, and through wired or wireless network and remote control component two -way communication connection. The utility model discloses through real -time monitoring and intelligent analysis and judgment, has realized clean room environment's all -round, intelligent, pre -judgment formula monitoring and guarantee, has avoided experiment pollution risk effectively, and has reached the effect of energy -conserving and loss -reducing.

The application discloses a gallium metal organic framework material, which is prepared through the following steps: (1) dissolving 2-amino terephthalic acid and itaconic acid in an organic solvent to obtain solution A; (2) dissolving gallium nitrate hydrate in an organic solvent to obtain solution B containing gallium ions; and (3) mixing solution A and solution B, heating under the condition of 50-70 DEG C for 3-5 hours, centrifuging and collecting the precipitate, and purifying to obtain the gallium metal organic framework material. The gallium metal organic framework material is constructed by combining itaconic acid and 2-amino terephthalic acid as a double ligand and taking gallium ions as a metal node, so that the technical problems of short biological half-life and low cell uptake efficiency of free itaconic acid are solved, the itaconic acid and the gallium ions are coordinated to play an anti-inflammatory and antibacterial role, to remove planktonic bacteria, and to have strong antibiofilm activity, and the gallium metal organic framework material can be used for preparing a drug for treating pyogenic arthritis.

The invention belongs to the technical field of high-throughput detection, and discloses a double-screening method and application of antibacterial and anti-biofilm active ingredients, the method comprises the following steps: dissolving a sample in a first solvent, carrying out liquid chromatography separation to obtain a separated fraction and obtain chromatographic data, taking part of the separated fraction, and carrying out mass spectrometric detection to obtain mass spectrometric data; collecting the other part of separated components to a microwell plate for vacuum drying, adding a bacterial liquid containing staphylococcus aureus, and culturing to obtain an activity test system; adding a first resazurin solution into the system, incubating, detecting the fluorescence intensity, removing planktonic bacteria, adding a second resazurin solution, incubating, and detecting the fluorescence intensity; correlating chromatography, mass spectrum and twice fluorescence intensity detection data to complete double screening of antibacterial and anti-biofilm active components. According to the antibacterial and anti-biofilm active component double-screening method provided by the invention, efficient integration of component separation, structural qualification and double-activity detection is realized, and the screening efficiency and accuracy of target active components in natural products are improved.

The utility model relates to a kind of bacteria sampling device, including sampler main body, sampler main body has negative pressure passage, the air inlet end of negative pressure passage is fixed first magnetic force column, still including collection cover, handheld column, hose, support pipe and placement tray, this sampler is divided into static fixed-point sampling and dynamic mobile sampling two states.Static fixed-point sampling state, applicable to single point long time continuous monitoring.Static fixed-point sampling state does not use hose and handheld column, dynamic mobile sampling state, airflow enters the drainage passage of handheld column along support pipe inner wall.After airflow is guided out through drainage passage, it is transported to the collection cover's collection end by hose.Hose flexibility ensures that air path is sealed when handheld column moves.Air enters the negative pressure passage of sampler main body from the collection cover flared end, planktonic bacteria are intercepted on the surface of petri dish.Support pipe and the collaborative design of drainage passage make airflow keep stable flow rate during moving.