The invention discloses a
mycobacterium tuberculosis medicament sensitive
phenotype detection method, which comprises the following steps of: 1) adding 2.5 to 6mul of 0.2 percent of
resazurin dissolved by using
methanol with
mass percentage concentration into the inner side of a tube cover of a sterile
centrifuge tube, and sterilizing the
centrifuge tube after the
methanol is volatilized; 2) inoculating 5 to 50mg of
mycobacterium tuberculosis to be detected into a 7H9-S culture medium, regulating the concentration of the
bacteria solution by adopting
turbidimetry, regulating the concentration of the
bacteria solution to the concentration of a Mcburney turbidimetric 1 tube by using
sterile water or 0.9-percent
physiological saline, and diluting the
bacteria solution by 20 times by using the 7H9-S culture medium; 3) inoculating 50 to 150mu of diluted bacteria solution into the
centrifuge tube of the step 1); 4) adding 100mul of anti-tubercular medicament solution diluted by 7H9-S and to be detected into the centrifuge tube of the step 3), wherein the concentration of the medicament to be detected is 0.01mug / ml to 2.5mg / ml; 5) after the centrifuge tube of the step 4) is covered closely, culturing the solution for 6 to 7 days at the temperature of 37 DEG C; and 6) inverting and oscillating the centrifuge tube of the step 5), and culturing the solution for 12 to 48 hours at the temperature of 37 DEG C after the solution becomes blue. Proved by experiments, in the
mycobacterium tuberculosis medicament sensitive
phenotype detection method and application of the
mycobacterium tuberculosis medicament sensitive
phenotype detection method in anti-tubercular medicament screening, the method has low
workload and short detection time; compared with a traditional L-J medium medicament sensitive detection method, the accuracy reaches 90 to 99 percent; and the method has good
biological safety, does not cause laboratory propagation of tuberculosis, is suitable for large-scale.