17 results about "Resazurin" patented technology

The invention provides a resazurin chromogenic stop buffer and application thereof, and belongs to the technical field of biological detection. The resazurin chromogenic stop buffer is a solution containing SDS and a buffer system, and the pH value of the solution is larger than or equal to 8. The invention aims to solve the problem of color fading during the existing resazurin color development, can effectively terminate the color development reaction, and ensures the stability and reliability of the color development result. According to the resazurin color development stop buffer, an environment-friendly formula and a process are adopted, and the burden on the environment is reduced. The technical breakthrough is expected to promote wide application of resazurin chromogenic reaction in the field of biotechnology, a more reliable and environment-friendly tool is provided for researches such as microbiological detection, cell activity detection and enzyme activity determination, and development of related scientific research and industries is further promoted.

The invention discloses a method for accelerating the color development speed of filamentous fungus resazurin and application of the method, and relates to the technical field of drug sensitivity detection. The invention provides a method for accelerating the color development speed of filamentous fungus resazurin, which comprises the following steps: S1, diluting a filamentous fungus bacterial liquid by using an MUELLER-HINTON culture medium, and adding the diluted filamentous fungus bacterial liquid into a to-be-detected drug hole for incubation; s2, performing suction filtration after incubation; s3, adding a color developing agent containing a catalyst for color development; s4, result interpretation; the catalyst in the step S3 is menadione. According to the MUELLER-HINTON culture medium, in the Aspergillus drug sensitivity detection process based on resazurin color development, after a catalyst is added, color development is accelerated, meanwhile, all medicine adding holes cannot be red, and the MIC value can be accurately obtained.

The invention discloses an application of a small molecule compound in preparation of a medicine for treating lymphoma, and the small molecule compound is resazurin sodium or 2-(3, 4-dihydroxybenzoyloxy) ethyl 3, 4-dihydroxybenzoyloxy. The application of the small molecule compound in preparation of the medicine for treating the lymphoma is characterized in that the small molecule compound is resazurin sodium or 2-(3, 4-dihydroxybenzoyloxy) ethyl 3, 4-dihydroxybenzoyloxy. Deoxyribose nucleic acid (DNA) methylation abnormity is one of important mechanisms of tumor generation and development, existing researches show that CDCA7 is highly expressed in lymphoma, CDCA7 knock-down can damage the invasion ability of lymphoma cells in vitro, and another research shows that CDCA7 protein is a key factor for maintaining methylation. According to the application disclosed by the invention, CDCA7 of a mouse B cell lymphoma cell line CH12F3 is knocked out, and the methylation degree of a Microsallite DNA region is found to be reduced, while the methylation degree of the Microsallite DNA region is also reduced when CH12F3 cells of which CDCA7 is not knocked out are cultured by using the small molecule compound disclosed by the invention. Therefore, the small molecule compound has a wide prospect in application to preparation of drugs for treating lymphoma.

The invention belongs to the technical field of drug sensitivity detection, and particularly relates to a method for increasing the drug sensitivity detection speed of saccharomycetes and application of the method. The method comprises the steps of bacterial liquid preparation, sample adding, incubation, thallus enrichment, color developing agent adding and result interpretation, ethylenediamine tetraacetic acid or salt thereof needs to be added before thallus enrichment, and drug action and color developing are carried out separately. The detection method provided by the invention can effectively accelerate the developing speed of resazurin, remarkably shortens the drug sensitivity detection time, and is beneficial to early accurate diagnosis and treatment of clinical fungal diseases.

Compositions comprising 2-hydroxycarboxylic acid for dysregulating the metabolism of planktonic bacteria and their use. This disclosure provides compositions for dysregulating the metabolism of planktonic bacteria, the compositions comprising preferred enantiomers of 2-hydroxycarboxylic acid. This disclosure further provides compositions for dysregulating the metabolism of planktonic bacteria, the compositions comprising preferred enantiomers of 2-hydroxycarboxylic acid and antimicrobial compounds. Dysregulation of the metabolism of planktonic bacteria can be measured, among other examples, by a decrease in the MIC of concomitant antimicrobial agents, an enhancement of in vivo antimicrobial effects, or aerobic respiration of metabolically active cells (reduction of non-fluorescent resazurin to highly fluorescent resorphine), which can lead to sensitization of planktonic bacteria to antimicrobial compounds and / or disruption of resistance of planktonic bacteria to antimicrobial compounds.

The application discloses a buffer for improving stability of resazurin. In view of the problem of resazurin instability under illumination in RPMI-1640 culture medium, the application provides a buffer containing a compound with structure I, the compound with structure I is reasonably combined with other components in the buffer, the illumination stability of the culture system is obviously improved, and the color of the buffer can be maintained unchanged for more than 5 hours under illumination.

The invention relates to the technical field of intestinal microecology, and particularly discloses an in-vitro fermentation system for rapid targeted screening, regulation and control of intestinal TMA generation, an establishment method and application, and the in-vitro fermentation system comprises the following components in parts by weight: 2-3 parts of yeast extract, 0.8-1.2 parts of L-cysteine, 1.5-2.5 parts of heme, 0.8-1.0 part of sodium chloride, 0.008-0.012 part of calcium chloride hexahydrate and 0.4-0.5 part of dipotassium phosphate. The feed is prepared from the following raw materials in parts by weight: 0.08 to 0.1 part of magnesium sulfate heptahydrate, 0.15 to 0.25 part of vitamin mixture, 7 to 9 parts of starch, 0.01 to 0.8 part of choline and 0.8 to 1.2 parts of resazurin. The method has the characteristics that the large-scale and high-flux active substance screening requirement can be met, and the TMA generation and related substrate metabolism quantitative control can be realized.

The invention provides a high-throughput screening method of a bacillus subtilis strain for inhibiting aspergillus flavus. The method comprises the following steps: activating bacillus subtilis and then carrying out single colony separation; numbering the obtained single colonies, respectively inoculating the single colonies into 96 deep-well plates for fermentation, centrifuging fermentation liquor, taking supernate, and passing the supernate through a membrane to obtain fermentation supernate; respectively adding each fermentation supernatant into a 96-well plate, and adding a developing working solution for incubation; the developing working solution is a mixed solution of an aspergillus flavus spore suspension and a resazurin solution; screening strains corresponding to holes with fluorescence intensity lower than that of the blank control group and color close to blue as primary screening strains; and carrying out secondary screening on the fermentation supernatant of the primary screening strain by using the developing working solution, and screening the bacillus subtilis strain with high aspergillus flavus inhibition activity according to the fluorescence intensity. Therefore, the bacillus subtilis single strain with high inhibitory activity on aspergillus flavus can be efficiently screened from a large number of samples.

The invention discloses a kit and a method for rapidly detecting the activity of reductase in cells. The kit comprises a solution A and a solution B, wherein the solution A is a PBS (Phosphate Buffer Solution) solution with the pH of 6.5 and the concentration of 20 mM And the solution B is 0.05 wt% of resazurin solution. The method comprises the following steps: pretreating a biological cell sample, reacting the pretreated biological cell sample with resazurin in an accurate reaction system, detecting by a microplate reader, and calculating the reductase activity of the sample. Based on the effect of resazurin as a redox indicator, the reductase determination kit is successfully prepared, the kit can be used for detecting different biological cell samples and presenting intuitive and accurate results, accurate reductase activity values are obtained by comparing the results with a standard curve of resazurin reduced by ascorbic acid, and the redox state of cells can be quickly mastered. The method is rapid and convenient, does not need complex and expensive instruments and equipment, can complete reductase detection in a short time, and is suitable for application scenes such as rapid detection of a large number of samples and the like.

The invention discloses a ruminococcus buchneri fermentation growth medium and a preparation method thereof. The growth medium is prepared from the following raw materials: a carbon source, a nitrogen source, inorganic salt, a reducing agent, a redox indicator, cooked beef granules and bovine rumen fluid, wherein the carbon source is resistant starch, and the concentration of the carbon source is 3.0-7.0 g / L; the concentration of the nitrogen source is 16.0-67.0 g / L; the reducing agent is L-cysteine hydrochloride, and the concentration of the reducing agent is 0.1-0.7 g / L; the redox indicator is resazurin, and the concentration of the redox indicator is 0.001-0.007 g / L; the volume ratio of the bovine rumen fluid is 5%-25%; the mass volume ratio content of the cooked beef granules is 10.0-40.0 g / L. By optimizing the formula of the culture medium, the growth efficiency and metabolism of the ruminococcus buchneri are remarkably improved, and the growth activity is prolonged, which is specifically reflected in multiple aspects such as thallus growth density, physiological functions and economy.

The application discloses a buffer for improving stability of resazurin. In view of the problem of resazurin instability under illumination in RPMI-1640 culture medium, the application provides a buffer containing a compound with structure I, the compound with structure I is reasonably combined with other components in the buffer, the illumination stability of the culture system is obviously improved, and the color of the buffer can be maintained unchanged for more than 5 hours under illumination.

The invention belongs to the technical field of high-throughput detection, and discloses a double-screening method and application of antibacterial and anti-biofilm active ingredients, the method comprises the following steps: dissolving a sample in a first solvent, carrying out liquid chromatography separation to obtain a separated fraction and obtain chromatographic data, taking part of the separated fraction, and carrying out mass spectrometric detection to obtain mass spectrometric data; collecting the other part of separated components to a microwell plate for vacuum drying, adding a bacterial liquid containing staphylococcus aureus, and culturing to obtain an activity test system; adding a first resazurin solution into the system, incubating, detecting the fluorescence intensity, removing planktonic bacteria, adding a second resazurin solution, incubating, and detecting the fluorescence intensity; correlating chromatography, mass spectrum and twice fluorescence intensity detection data to complete double screening of antibacterial and anti-biofilm active components. According to the antibacterial and anti-biofilm active component double-screening method provided by the invention, efficient integration of component separation, structural qualification and double-activity detection is realized, and the screening efficiency and accuracy of target active components in natural products are improved.

The present disclosure belongs to the field of microbial technology, and discloses a biocatalytic method for enhancing arsenic methylation and volatilization capabilities of Clostridium and a use thereof. Specifically, the present disclosure discloses use of resazurin in enhancing arsenic methylation capability of bacteria. The present disclosure proposes for the first time that resazurin may promote the functions of bacteria (e.g. Clostridium sporogenes) in production and volatilization, as well as cell adsorption of methyl arsenic. Experimental results demonstrate that exogenous addition of resazurin may significantly improve the methylation and volatilization effects of inorganic trivalent arsenic by Clostridium sporogenes, providing an effective means for the remediation of arsenic-contaminated soil and water bodies.

This invention discloses a lignin-degrading microorganism, its isolation method, and its application, relating to environmental microbiology technology and the field of biodegradation. The isolation method includes: enriching the sample to be isolated using alkaline lignin as the sole carbon and energy source; then using morphological recognition-based single-cell sorting technology and resazurite staining to screen for active core strains that grow well in a single lignin culture medium and can dissolve lignin precipitates; and finally, sequencing identification to obtain a pure microbial strain that degrades lignin. This isolation method can effectively isolate lignin-degrading microorganisms, is simple and efficient, and is suitable for large-scale applications. Furthermore, this invention identifies [specific microorganisms] based on this isolation method. Phytobacter ursingii It is an active core strain for degrading lignin, with outstanding lignin degradation efficiency, providing an important strain resource for developing microbial-based biological treatment technology for lignin waste.

The application is suitable for the field of protein detection, and provides a microprotein detection method, comprising the following steps: step one, preparing a protein solution, and adsorbing the protein solution through a polystyrene hole plate, and then washing; step two, synthesizing titanium dioxide nanoparticles by a solvothermal method, and adding the titanium dioxide into the protein solution, so that the titanium dioxide is combined with the hole plate protein, and washing after the combination; step three, adding a chromogenic solution into the hole plate, and irradiating the chromogenic solution through ultraviolet light, so that the resazurin in the chromogenic solution is converted into resorcinol; and step four, reading and recording the fluorescence value of the hole plate through an enzyme label instrument. The research proposes a new microprotein detection method, realizes signal amplification through a catalytic reaction, has the characteristics of convenient operation, high speed and low cost, and takes urine protein detection as an example to verify the feasibility, sensitivity and stability of the method, has high practical application value, and provides a new solution for clinical urine protein detection.

The invention relates to the technical field of microbial preparations for veterinary use, in particular to an anaerobic preparation process of a feline coprophilous fungus capsule, and the whole process of coprophilous fungus collection, pretreatment, freeze-drying and filling is performed in an anaerobic workstation; in the coprophilous fungus pretreatment stage, a pre-reduction PBS solution containing taurine, cysteine and resazurin is adopted; a composite freeze-drying protective agent containing taurine, reduced glutathione and other components is adopted during freeze-drying, through collaborative optimization of the process, the number of viable bacteria and the flora activity in the cataliella felina capsule are remarkably improved, and the cataliella felina capsule remarkably shows a functional effect superior to that of a traditional process in an intestinal flora regulation experiment.

The present application relates to the field of biotechnology, especially to a buffer for improving stability of resazurin, and provides a buffer containing a compound with structure I, and each component is reasonably matched, the buffer is added to a cell culture medium containing resazurin indicator, and the light stability of the culture system is obviously improved, and the color does not change for more than 5 hours under light.