57 results about "Biological evaluation" patented technology

The invention discloses a four-color fluorescence labeling reversible terminal and a use thereof in DNA (Deoxyribonucleic Acid) sequencing. The structural formula of the reversible terminal is as shown in a formula (I) in the specification, wherein R1 is triphosphate; R2 is H or OH; the basic group is U, C, A, G or derivatives thereof; the connecting unit is a bifunctional compound which is breakable under a mild condition; the fluorescence group is one selected from a combination of BODIPY, fluorescein, rhodamine, coumarin, xanthene, cyanin, pyrene, phthalocyanine, alexa, squarene dye and an energy transferring dye, and derivatives thereof. The reversible terminal provided by the invention can be used for DNA single-molecule sequencing; simultaneously, raw materials required by the synthesis of the reversible terminal provided by the invention are simple and easy to get and the synthesis process of the reversible terminal is completely involved with conventional chemical reactions, so that the four-color fluorescence labeling reversible terminal can be popularized and utilized to a large scale; biological evaluation results indicate that the reversible terminal is capable of completely meeting the biochemical reaction requirements of high-flux sequencing and has a good practical prospect.

Microfluidic embryo scaled channels (14) for handling and positioning embryos provide the opportunity to evaluate and treat embryos in improved manners. Fluid flow is used to move and position embryos within microfluidic channels and channel geometrics may be used to place embryos at specific locations. Surface properties and compliance (deformation) properties of embryos are evaluated as a predictor of viability. The microfluidic channels provide the opportunity for fine controls of pressure to conduct various evalutions at forces slightly below which damage to embryos is known to occur. Measurement of the distance and / or which embryos roll in a same pressure gradient microfluidic channel provides information, with healthy embryos traveling slower or a shorter distance as they demonstrate more stiction to channel walls. Positioned at a constriction (14a, 14b, 24, 26), health embryos also appear to deform less than unhealthy embryos that are more readily pulled into a constriction. In addition, healthy embryos appear to resume their shape better. Fluid from microfluidic channels is easily collected downstream without altering the embryo environment, providing a better opportunity for chemical analysis of fluid chemical analysis than convention manual handling and sampling techniques. Zona pellucida removal of mammalian embryos is achieved as embryos are moved through flow to a precise location where lysing agent can be washed over the embryo to achieve zona removal. Cumulus removal is realized with a series of constrictions to cut cumulus followed by fluid flows to remove cut cumulus from the embryo.

A low-odor and high-toughness ultraviolet curing adhesive comprises the following raw materials in percentage by weight: 25-50% of acrylic modified polyurethane resin, 45-70% of an acrylic monomer diluent, 15-45% of a toughening monomer, 2-3% of a silane coupling agent, 2-3% Of a photoinitiator, 0-0.2% of a polymerization inhibitor and 0.1-0.5% of other auxiliaries; compared with the prior art, the low-odor and high-toughness ultraviolet curing adhesive has the beneficial effects of being small in odor, high in toughness, resistant to ethylene oxide sterilization and damp-heat aging, and qualified in biological evaluation performance, and can be used for bonding among common polymer materials such as PVC, PC, ABS and k-resin used in medical instruments.

The invention relates to a method for rapidly detecting on-site catering safety, and the method comprises the following steps: constructing a zebra fish toxicity evaluation model, and evaluating the eating safety of catering food by detecting a zebra fish movement function, a cardiovascular function or an intestinal peristalsis function. According to the rapid detection method established by the invention, based on the thinking model of overall biological evaluation, the safety of catering food is integrally evaluated by qualitatively or quantitatively observing the transportation behavior, heartbeat and blood flow and diarrhea conditions of zebra fish, and the detection thought is not limited by the toxic influence of a traditional chemical detection method on a single component. The detection method provided by the invention is good in universality, can systematically and comprehensively evaluate the biological safety of diet in catering industries including restaurants, fast food restaurants, snack shops, canteens and the like, and has the advantages of quickness, convenience, reliability, good predictability and the like which cannot be compared by a traditional detection method.

Disclosed are a serious of 2′,5′-dimethoxychalcone derivatives for treating cancer, wherein 2,5-dimethoxyacetophenone and methyl 4-formylbenzoate are condensed to form 4-carboxyl-2′,5′-dimethoxychalcone (compound 1), which is further reacted with alkyl halides or amines to synthesize the chalcone derivatives of compounds 2-17. In addition, 2,5-dimethoxyacetophenone is reacted with 5-formyl-2-thiophenecarboxylic acid to form compound 18 (3-(3-thiophene)carboxyl-1-(2,5-dimethoxyphenyl)prop-2-en-1-one). The synthesized 2′,5′-dimethoxychalcone derivatives can be acted as microtubule-targeted tubulin-polymerizing agents.

The invention discloses application of SLC2A1 expression to preparing a kit for detecting the fertility of oocytes, and a method for evaluating the physiological function of the oocytes, and belongs to the technical field of the cytology and biology. The application is uppermost characterized in that SLC2A1 is glucose transport protein the most expressed in the glucose transport protein family inthe oocytes, the SLC2A1 expression amount is in positive correlation with the fertility in the oocytes, the unfertilized oocytes present the missing and remarkable dropping of SLC2A1 genes, the kit for detecting the fertility of the oocytes detects and analyzes the expression of all the currently-known members in the same SLC2A family in the oocytes through the real-time fluorescent quantitative reverse transcription polypeptide chain reaction (qRT-PCR) method, it is found that the SLC2A1 is related to the quality and fertility of the oocytes, and the effective biological evaluation means is provided for controlling the quality of the assisted reproductive technology.

The invention discloses an indenopyrazole small-molecule tubulin inhibitor, which is characterized by having a structure represented by general formula I:wherein R represents NH2 or NHOH; the invention also discloses a preparation method of the indenopyrazole compound, or pharmaceutical salts thereof. The compound of the present invention is an indenopyrazole small-molecule tubulin inhibitor having a novel structure, and has very strong proliferation inhibition activity to human hepatocellular carcinoma (HepG2) cells, human prostate carcinoma (PC3) cells, human cervical carcinoma (HeLa) cells, human breast adenocarcinoma (MCF-7) cells, and human leukemia (K562) cells; the compound is similar to colchicine in mechanism of action, and thus capable of inhibiting tubulin polymerization; the compound is significant for enhancing the specificity and effectiveness of drugs, reducing toxic and side effects, preventing drug tolerance, and so on.

A biological evaluation method for rational and simply evaluating biological harmfulness or usefulness of a great number of natural and artificial chemicals, foods, etc. This method comprises adding a known amount of 2′-deoxyguanosine (dG) to a solution containing a test substance (drug, pesticide, functional food, etc.), optionally applying UV light and / or adding active oxygen generator, then quantifying 8-hydroxy-2′-deoxyguanosine (8OHdG) in the solution, and evaluating the toxicity or usefulness of the test substance according to the 8OhdG content (a higher 8OhdG content indicates a higher harmfulness of the test substance while a lower 8OhdG content indicates a lower harmfulness or an usefulness thereof) The invention also provide an apparatus for advantageously performing said biological evaluation method and an antioxidant preservative solution to be used in this apparatus.