38 results about "Biological evaluation" patented technology

The invention discloses an intelligent detection and analysis system for biological evaluation data of a medical instrument, and relates to the technical field of data processing, and the system comprises an image acquisition module which carries out the rapid imaging of the initial physical planking state of cells in a microwell plate; the image processing and feature quantification module is used for identifying the cell object and extracting a plurality of physical operation quality (POQ) feature parameters representing a population space distribution mode of the cell object; the quality evaluation and early warning module is used for comparing the POQ fingerprint with a preset quality baseline in real time and generating an early warning signal when the deviation exceeds a threshold value; and the data management and trend analysis module is used for continuously recording and analyzing POQ fingerprint historical data associated with the automatic pipetting equipment. According to the method, the physical operation quality is monitored at the experiment starting point, so that the accuracy, the reliability and the experiment efficiency of biological evaluation data of medical instruments are improved, the resource waste and the failure risk caused by initial inoculation errors are effectively reduced, and a way is provided for intelligent management of automatic equipment.

The invention is suitable for the field of medical image analysis, and provides a tumor pathological image segmentation method based on a U-Net neural network, and the method comprises the steps: extracting the gray, texture and shape multi-dimensional features of tumor cells through a U-Net encoder, constructing a feature cluster, building an invasion direction probability model based on the cluster, analyzing the morphological features of a necrotic region, and speculating the growth speed. And dynamically adjusting the segmentation sensitivity threshold of the decoder, and finally outputting a three-channel segmentation map containing a tumor core region, a false envelope invasion region and a capillary invasion region. According to the scheme, through multi-dimensional feature fusion and biological behavior modeling, precise characterization of tumor heterogeneity is achieved, the adaptability of a segmentation model to different invasion active areas is improved through a dynamic sensitivity regulation mechanism, a segmentation result with anatomical positioning and biological evaluation values is provided for clinic, and the segmentation accuracy is improved. And diagnosis and treatment decision of tumors are effectively assisted. The method is easy and convenient to operate and high in automation degree and has remarkable clinical application value.

The invention discloses a water ecological health assessment method based on a drinking water source water ecological health assessment system, and overcomes the defects that a water ecological environment investigation and assessment system in the prior art is not suitable, the algal toxin content is high, early-stage fish data is lacked, and drinking water source water storage branches are all secondary branch areas. The evaluation method comprises the steps that S1, a based evaluation system comprises a water quality evaluation layer, a habitat evaluation layer and a biological evaluation layer with the weights of X1, X2 and X3, the weights of the three evaluation layers are determined according to the water ecological condition of a to-be-monitored area, and X1 + X2 + X3 = 1; s2, constructing a water ecological health assessment system of the to-be-monitored area; s3, monitoring index sub-items of water ecology of the to-be-monitored area; and S4, evaluating the water ecological health of the to-be-monitored area. According to the evaluation system and method, the water quality evaluation layer, the habitat evaluation layer and the organism evaluation layer are arranged, the weight is set according to the water ecological condition of the to-be-monitored area, and the evaluation method is more scientific.

The invention discloses a low-drunkenness and high-comfort Luzhou-flavor liquor body design method, which comprises the following steps: performing biological evaluation on Luzhou-flavor liquor on the market through a model animal drunkenness model, evaluating the low-drunkenness Luzhou-flavor liquor, determining the skeleton flavor content, the acid-ester ratio, the alcohol-ester ratio and the total flavor proportion, and determining the content of the skeleton flavor, the acid-ester ratio, the alcohol-ester ratio and the total flavor proportion. Carrying out low-drunkenness and high-comfort Luzhou-flavor liquor body design to form a primary product; the primary product is subjected to sensory evaluation, flavor physical and chemical analysis and consumer drinking comfort test, the skeleton flavor structure of the primary product is optimized and adjusted, the final requirements of low drunkenness and high comfort are met, the sensory flavor and physical and chemical quality of the product are guaranteed, the requirement of consumers for healthy and comfortable drinking is met, and the product has good market prospects. The change of white spirit body design from single pursuit of aroma taste to emphasis on drinking comfort of consumers is initiated, and the method also has positive guiding significance on healthy development of consumer markets.

A series of non-peptide mu opioid receptor (MOR) selective modulators is provided. The compounds have the general formula (I) where R is (II) and where one of a, b, c, d, e or f is the point of attachment of indole ring R to the epoxymorphinan skeleton; at least one of the atoms at positions a, b, c, d, e and f is N; the remaining atoms at positions at a, b, c, d, e and f are CH; and * is a chiral carbon. The compounds are generally MOR antagonists and are used to treat disorders related to opioid receptor functions such as opioid addiction and opioid overdose, for the treatment of neurological diseases and for the treatment of pain, without causing constipation.

The present invention describes the synthesis and biological evaluation of new substituted pyridine derivatives as foxj1 activity enhancers. In embodiments, novel pyridine derivatives were synthesized and the ability thereof to enhance foxj1 activity was evaluated. The novel pyridine derivatives synthesized in the present invention not only exhibited excellent activity but also exhibited excellent metabolic safety and pharmacokinetic profile in a Tg (foxj1: egfp) transgenic zebrafish animal model. In addition, the novel pyridine derivatives synthesized in the present invention enhanced cilia-promoting capability in a motile ciliated cell differentiation experiment in which mouse tracheal epithelial cells (mTECs) were isolated from the airways of mice and cultured by ALI. As a result, a series of foxj1 activity enhancers with substituted pyridine skeleton has the potential to be developed as preventive and therapeutic agents for respiratory diseases.

The application discloses a rat fixing device based on biological evaluation of medical instruments. The device comprises an operation panel, a fixing cover, a sliding sheet and a sliding groove. A rectangular opening is designed in the center of the base of the operation panel, which is specially used for abdominal cavity injection operation of rats. The rat fixing cover is divided into four sections and is arranged on the operation panel. The two sides of the fixing cover are provided with the sliding sheets. The shape of the two sliding sheets after being spliced together is the same as that of the fixing cover. The lower end of the sliding sheet is in contact with the operation panel, and the sliding groove is arranged at the contact part. The rat fixing device can effectively adjust the size, so that rats with different body types can be effectively fixed, and the smooth operation of the test is ensured. The device is convenient to operate and stable in rat fixing.

This invention belongs to the field of biomedical materials technology, specifically disclosing a core-shell microneedle, a core-shell microneedle patch, its preparation method, and its application. Through structural design and carrier material optimization, this invention provides a core-shell microneedle with a programmed sequential release of tannic acid in the outer shell and verteporfen in the core layer, successfully matching the dynamic physiological rhythm of wound healing and ensuring effective wound repair. In vitro and in vivo biological evaluations have confirmed that the core-shell microneedle patch prepared using the core-shell microneedles of this invention possesses excellent antibacterial, antioxidant, and immune microenvironment-regulating abilities. It can effectively induce macrophage polarization towards the M2 type, specifically inhibit YAP protein expression, and significantly accelerate the closure process of infected wounds. From both upstream immune regulation and downstream fibrosis inhibition levels, it dually blocks the pathological scar formation pathway, ultimately achieving high-quality, scar-free regenerative repair of infected wounds.

The application discloses a furan amide compound and application thereof, and belongs to the technical field of medicines.The structural general formula of the furan amide compound is shown as formula (I).The application provides a novel furan amide AR antagonist, the compound and the derivative thereof have obvious antagonistic activity on an androgen receptor, and exhibit good biological activity in in-vivo and in-vitro biological evaluation, the androgen receptor antagonistic activity is better than that of enzalutamide, the compound is effective in an AR F877L / T878A double-point mutant cell model resistant to enzalutamide, the activity is better than that of darolutamide, and the compound has good safety.Therefore, the compound can be applied to the treatment of diseases related to the androgen receptor as an androgen receptor antagonist, including but not limited to the treatment of prostate cancer, breast cancer, ovarian cancer and the like.

The invention belongs to the field of medical supplies, and discloses a medical fever cooling patch and a preparation method thereof. The medical fever cooling patch is prepared from the following raw materials in percentage by mass: 0.1%-2% of carrageenan, 0.02%-2% of potassium chloride, 0.02%-2% of konjac glucomannan, 0.02%-2% of maltodextrin, 0.01%-2% of potassium citrate, 0.01%-2% of sodium chloride, 0.02%-2% of edible glucose, 0.01%-2% of xanthan gum, 0.1%-10% of a carboxymethyl chitosan-modified activated zeolite composite material, 0.00001%-0.01% of a coloring agent and the balance of water. The water content of the fever cooling patch can be larger than 90%, and the cooling degree, the continuous cooling time, the good viscosity and other performance are effectively guaranteed. The electronegativity of CMCS in the carboxymethyl chitosan-modified activated zeolite composite material and the electronegativity of the modified activated zeolite have a synergistic effect, so that no preservative is added, the cytotoxicity of the zeolite is greatly reduced, the biological evaluation of the medical fever cooling patch meets the requirements, and the survival rate of L929 cells is greater than 90%.

The utility model provides a biological evaluation implantation device for a medical instrument. The biological evaluation implantation device comprises an injection tube and an injection core, one end of the injection tube is open, the other end of the injection tube is sharp, so that the injection tube can pierce into tissues conveniently, and a push-out opening communicated with the injection tube is formed in the side wall of the other end of the injection tube; the injection core is inserted into the injection pipe in a sliding mode through an opening in one end of the injection pipe. The sharp end of the injection tube penetrates into other soft tissues such as target muscular tissues, subcutaneous tissues and the like of an experiment; according to the invention, the injection core is controlled to slide, so that the implant is pushed to slide in the injection tube, and the implant can be pushed out through the push-out opening in the side wall of the injection tube, so that the implant is simply and quickly fed into an expected implantation part in a manner of minimum damage and uniform implantation manner, and the biocompatibility of the implant can be evaluated.

The invention belongs to the field of sample analysis, and provides a mass spectrum system and method integrating online pretreatment and dynamic biological evaluation. The system comprises a sample on-line pretreatment part which is used for obtaining a sample mother solution to be analyzed and separating and preparing concerned components in the sample mother solution; the dynamic biological evaluation part is used for performing multi-channel cell culture and real-time microscopic observation on concerned components in the sample; the dynamic biological evaluation part outputs at least two paths, and one path is connected with the multi-source chromatography-mass spectrometry analysis part so as to finish dynamic biological evaluation for analyzing related components at a cellular level; the other path is synchronously connected to a functional target plate site, and matrix-assisted laser desorption mass spectrometry and surface desorption mass spectrometry are carried out; the system control and data processing part is used for carrying out qualitative and / or quantitative analysis on the components of the metabolite of the dynamic biological evaluation part, and detecting sample injection mass spectrograms, sample difference metabolites and protein molecules in all treatment links of the sample.

The application discloses a kind of PROTAC molecules with crizotinib as target head, preparation method and application, belong to the field of biological medicine;Preparation method includes: chloro carboxylic acid, thionyl chloride and anhydrous DMF are mixed, after heating reflux reaction, remove thionyl chloride, obtain acyl chloride;Pomalidomide, acyl chloride, organic solvent are mixed and reacted, then filtered to obtain intermediate M;Intermediate M, crizotinib, Na2CO3 are mixed and reacted, then filtered to obtain the PROTAC molecule with crizotinib as target head.The in vitro biological evaluation shows that the new PROTAC molecule can efficiently and selectively induce ubiquitination and degradation of target protein, and exhibits significant antiproliferative activity in drug-resistant tumor cell models and animal models;The application proves that the PROTAC molecule has great potential in overcoming drug resistance of traditional inhibitors, and provides a promising lead compound for developing new therapies for treating refractory diseases such as cancer.

The invention belongs to the technical field of medical implant preparation, and particularly relates to a preparation and biological evaluation method of a porous titanium alloy medical implant. The preparation method of the porous titanium alloy medical implant comprises the following steps: S1, preparing a porous titanium artificial tooth root implant; s2, preparing a hydroxyapatite coating of the porous titanium artificial tooth root implant by adopting an electrochemical method; s3, carrying out fatigue test on the porous titanium artificial tooth root implant with the hydroxyapatite coating; s4, preparing a composite nano coating of the porous titanium artificial tooth root implant by adopting an electrochemical method; and S5, carrying out physicochemical and biological analysis on the obtained porous titanium artificial tooth root implant with the composite nano coating. The composite nano coating is applied to surface modification of the porous titanium alloy, the advantages of respective technologies are exerted, osteoblast proliferation and differentiated protein molecules can be remarkably induced, bone induction and bone conduction are enhanced, and bone defect repair and angiogenesis are promoted.

The invention discloses an aromatase activity determination method for a composition containing rehmannia, which comprises the following steps: (1) constructing an aromatase activity reaction system and a control system to respectively obtain an aromatase activity reaction solution and a control solution; (2) preparing a blank solution; (3) taking a proper amount of the aromatase active reaction solution, the contrast solution and the blank solution, and carrying out high performance liquid detection to obtain a detection result; and (4) obtaining aromatase activity information of the composition containing the rehmannia according to a detection result, the composition containing the rehmannia comprises prepared rehmannia root, wine-processed fructus corni, moutan bark, Chinese yam, poria cocos and rhizoma alismatis. The determination method disclosed by the invention is simple, convenient, rapid, accurate and reliable, can be used for biological evaluation of the quality of the composition containing the rehmannia glutinosa, and is expected to be applied to quality control work of the composition containing the rehmannia glutinosa.

A method for determining an aberrancy in at least one of a body and a brain. The method includes positioning a sensor on a brain thermal tunnel of a human and measuring a thermal output of the brain thermal tunnel. The sensor sends a signal representing the thermal output to a controller which performs a frequency analysis on the thermal signal to identify power peaks in the frequency range. A slope of a line drawn through at least two of the power peaks is determined, and exceeding a predetermined non-zero slope indicates a medical condition of the human.

The invention discloses an adjustable mainstream smoke monitoring mouse smoke addiction device and a working method thereof in the technical field of biological evaluation of smoking addiction, and aims to solve the problems that a device and an evaluation system related to smoke monitoring animal addiction are lacked in the prior art, and the degree and mechanism of smoke induced addiction are inconvenient to explore. The device comprises a flue gas exposure box, a position preference box and a camera, the flue gas exposure box is communicated with the position preference box through a blocking mechanism, the flue gas exposure box is communicated with a smoking machine through a gas inlet pipeline, an air pipeline is arranged on the gas inlet pipeline, the flue gas exposure box is communicated with a sucking pump, and a flue gas quality detector is arranged in the flue gas exposure box; according to the method, different smoke mass concentrations are adopted to monitor the mouse addiction, the established smoke addiction withdrawal model can explore the degree and mechanism of smoke addiction from multiple aspects such as the addiction degree and the withdrawal symptom, and the method plays an important role in exploring the effect and mechanism of smoking addiction.

The invention belongs to the technical field of medicinal chemistry, and discloses a 7-oxabicyclo [2.2. 1] heptane-2, 3-dicarboxylic acid derivative and an application of the 7-oxabicyclo [2.2. 1] heptane-2, 3-dicarboxylic acid derivative in preparation of a protein phosphatase 5 degradation agent. The compound can directly target PP5 and induce the PP5 to degrade, has the advantages of novel structure and excellent physical and chemical properties, and shows high selectivity to PP5. In various biological evaluations, the molecules all show remarkable anti-proliferative activity and degradation effect.

The present invention provides a method, system and device for evaluating the effectiveness of biodiversity restoration at an engineering scale, wherein the method comprises: obtaining basic data for biological restoration evaluation, obtaining evaluation indicators based on the basic data for biological evaluation; standardizing the evaluation indicators to obtain standard evaluation indicators; obtaining a biological restoration evaluation index based on the standard evaluation indicators; and evaluating the effectiveness of diversity restoration based on the biological restoration evaluation index. The present invention uses arthropods with rapid biological recovery as indicator species, optimizes the method for evaluating the effectiveness of biodiversity restoration at an engineering scale, develops a biological restoration evaluation index model, and realizes quantitative evaluation of the effect of biodiversity restoration.

The invention discloses a pH-responsive drug delivery agent based on a copper-based metal organic framework as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The delivery agent is prepared by taking MOF-199 as a carrier and loading an anti-cancer drug 5-fluorouracil (5-Flu) through an in-situ stirring method. The preparation method is simple, and the drug loading efficiency is high. The delivery system realizes intelligent drug release by using the acidic characteristic of a tumor microenvironment: under the simulated tumor acidic condition of pH 5.0, the cumulative drug release rate within 50 hours reaches 74.64%, which is significantly improved compared with a neutral environment (pH 7.4); the release mechanism of the compound is derived from acid-triggered Cu-O bond hydrolysis and phosphate radical competitive coordination. Biological evaluation shows that the drug carrier has higher than 80% of high biocompatibility for normal cells (Raw264.7), and has a remarkable inhibition effect on proliferation of meibomian adenocarcinoma cells (SCG) at the same time. The invention provides a new strategy for developing an anti-cancer drug delivery system with strong targeting property and high safety.

The invention provides a preparation method of a diacylglycerol kinase inhibitor, which comprises the following steps: S1, reacting 4-fluorobenzyl bromide with magnesium under inert gas by taking iodine as an initiator to obtain a Grignard reagent B; s2, the Grignard reagent B and N-tert-butyloxycarbonyl-4-piperidone are subjected to an addition reaction, and an intermediate C is obtained through hydrolysis and deprotection; s3, carrying out Heck coupling reaction on the intermediate C, 4-fluorophenyl trifluoromethanesulfonate and a catalyst in the presence of alkali to obtain an intermediate D; s4, carrying out substitution reaction on the intermediate D and chloroethanol in the presence of alkali to obtain an intermediate E; s5, carrying out Mitsunobu reaction on the intermediate E to obtain an intermediate F; s6, carrying out addition reaction on the intermediate F and N, N '-thiocarbonyldiimidazole to obtain an intermediate G; and S7, carrying out cyclization reaction on the intermediate G, 2-methyl aminobenzoate and alkali to obtain the diacylglycerol kinase inhibitor R59949. The synthesis route disclosed for the first time has the characteristics of mild reaction conditions, reasonable design, cheap and easily available raw materials, high yield and the like, and provides theoretical support for biological evaluation and clinical application of R59949.

The invention relates to the technical field of biological evaluation of medical instruments, in particular to a microfiltration membrane dynamic extraction-based cytotoxicity detection method which comprises the following steps: preparing a particle-containing gel sample to be detected into an extraction system; constructing a non-contact dynamic co-culture model containing a microfiltration membrane and cells; adding the leach liquor into the upper chamber, and carrying out dynamic leaching; cytotoxicity detection is carried out in the lower chamber. Particles are accurately intercepted through the micro-filtration membrane, detection errors caused by particle interference are avoided, the detection accuracy is improved, the authenticity of a detection result is ensured, 'false positive 'caused by particle interference in a traditional method is avoided, effective components of extract liquid are not affected by filtration, and a'micro-filtration membrane double-cavity dynamic co-culture system' is constructed; the slow release process of the material in tissue gaps after clinical injection is simulated, and dynamic monitoring of aging toxicity is achieved.

The invention discloses a preparation method and application of a pH response type drug delivery agent based on a zirconium-based metal organic framework. An MOF-808 carrier is synthesized through a hydrothermal method, the MOF-808 carrier is activated and then combined with an anti-cancer drug 5-fluorouracil (5-Flu) through an in-situ stirring method, the drug loading amount reaches 33.28% under optimized conditions, and the encapsulation efficiency is 66.55%. The delivery system utilizes the high specific surface area and Zr-O bond stability of MOF-808 to realize efficient drug loading and pH controlled release: under the acidic condition (pH 5.0) of a simulated tumor microenvironment, the accumulated drug release rate within 50 hours reaches 55.56% and is improved by 16.1% compared with a neutral environment (pH 7.4), and the release mechanism is derived from Zr-O bond hydrolysis and phosphate radical competitive coordination triggered by acidity. Biological evaluation shows that the carrier has higher than 80% of high biocompatibility for normal cells (Raw264.7), and meanwhile, proliferation of liver cancer cells (HepG2) is remarkably inhibited. The preparation method is simple in process and high in drug loading efficiency, has the advantages of tumor microenvironment targeted drug release and biological safety, and provides a new strategy for precise delivery of anti-cancer drugs.

This invention belongs to the field of pharmaceutical technology, specifically relating to a tetravalent platinum complex based on cinnamic acid, its preparation method, and its application. This invention is the first to introduce a cinnamic acid group into the structure of a tetravalent platinum complex, designing and synthesizing a series of novel platinum-based anticancer prodrugs, namely cinnamic acid-platinum (IV) complexes. Biological evaluation shows that these complexes exhibit stronger anticancer activity than classic divalent platinum drugs and good selectivity for cancer cells, demonstrating the potential of highly effective, low-toxicity, and drug-resistant novel anticancer drugs.

This invention is in the field of chemistry and contemplates pyridinium aziridine and aryl aziridine compounds, methods for synthesis, target identification, and biological evaluation of a novel class of chemical compounds for covalent targeting of aspartate and glutamate residues on disease-relevant proteins to expand the scope of the currently accessible druggable proteome and pharmaceutical compositions, such as protein inhibitors, and methods of use thereof.

The invention discloses an aroma molecule for reducing stimulation feeling of a respiratory tract and a biological evaluation method thereof, the aroma molecule is benzyl acetone or a structural analogue thereof, and the structural analogue comprises the following compounds: benzylidene acetone, phenethyl acetate, methyl salicylate, ethyl phenylacetate, benzyl butyrate and phenethyl butyrate. The biological evaluation method comprises the following steps: constructing at least one cell model of an overexpression TRP channel; preparing a to-be-detected aroma molecule solution and applying the to-be-detected aroma molecule solution to the cell model to evaluate the influence of the to-be-detected aroma molecule solution on the TRP channel; the influence of aroma molecules on the respiratory tract stimulation feeling is comprehensively evaluated by detecting the fluorescence intensity change of calcium ions in a cell model and observing the cough reaction and the concentration change of inflammatory factors IL-6 and IL-8 in an animal model. The method provided by the invention can be used for excavating and discovering key components of the tobacco flavor and flavor for improving the respiratory tract comfort, and meanwhile, the method can be used for evaluating the effect of the tobacco flavor and flavor for reducing the respiratory tract stimulation feeling.

The present disclosure provides technologies for predicting a phenotype of a microbial cell using machine learning models trained using high-content imaging data (HCI). Also provided are methods of engineering a microbial cell to possess a phenotype of interest. Example phenotypes include the production of a target compound or biomolecule of interest. The provided technologies are useful for the efficient biomanufacturing of target compounds.

The present invention relates to an in vitro cell test evaluation method suitable for evaluating the ultraviolet protection effect of sunscreen cosmetics, comprising (1) sunscreen cosmetic sample processing; (2) cell culture and cell inoculation; (3) UVA irradiation determination; (4) quantitative detection of ROS by an enzyme marker. This method uses the cell oxidative damage caused by long-wave ultraviolet radiation as a theoretical basis, and uses cells cultured in vitro as materials to establish an in vitro biological evaluation system that is different from the traditional sunscreen cosmetic evaluation method. An in vitro biological detection device innovatively applied in the experiment is scientifically and reasonably designed, has many advantages such as fast and simple operation, low cost, time saving, avoidance of operation pollution, relatively good data repeatability, etc., is safe to use, accurate in detection, and effectively conducts a scientific, reasonable and correct evaluation of the efficacy of sunscreen cosmetics, providing technical guarantee for the human safety and efficacy evaluation of sunscreen cosmetics.

The utility model belongs to the field of sample analysis, and provides a multi-source mass spectrum sample testing system which comprises an online pretreatment unit, a dynamic biological evaluation unit and a multi-source chromatography-mass spectrum analysis unit, the online pretreatment unit comprises at least two parallel extraction branches, and all the extraction branches are connected with the high-speed counter-current chromatograph through a first switching valve; the high-speed counter-current chromatograph is respectively connected with the dynamic biological evaluation unit and the multi-source chromatography-mass spectrometry analysis unit through a second switching valve; the dynamic biological evaluation unit comprises a multi-channel injection pump module, a micro-fluidic chip / organ chip cell culture module and a micro solid-phase extraction module; the input end of the multi-channel injection pump module is respectively connected with the high-speed counter-current chromatograph and the cell culture fluid vessel, and the output end of the multi-channel injection pump module is connected with the micro-fluidic chip / organ chip cell culture module; the input end of the micro solid-phase extraction module is connected with the micro-fluidic chip / organ chip cell culture module, and the output end of the micro solid-phase extraction module is connected with the multi-source chromatography-mass spectrometry unit.