Method for rapidly detecting on-site catering safety

Abstract

The invention relates to a method for rapidly detecting on-site catering safety, and the method comprises the following steps: constructing a zebra fish toxicity evaluation model, and evaluating the eating safety of catering food by detecting a zebra fish movement function, a cardiovascular function or an intestinal peristalsis function. According to the rapid detection method established by the invention, based on the thinking model of overall biological evaluation, the safety of catering food is integrally evaluated by qualitatively or quantitatively observing the transportation behavior, heartbeat and blood flow and diarrhea conditions of zebra fish, and the detection thought is not limited by the toxic influence of a traditional chemical detection method on a single component. The detection method provided by the invention is good in universality, can systematically and comprehensively evaluate the biological safety of diet in catering industries including restaurants, fast food restaurants, snack shops, canteens and the like, and has the advantages of quickness, convenience, reliability, good predictability and the like which cannot be compared by a traditional detection method.

Description

technical field [0001] The invention belongs to the technical field of on-site rapid detection (POCT), in particular to a method for rapidly detecting the safety of on-site catering, which is an evaluation technology for on-site rapid detection of the catering industry. Background technique [0002] Today, when everyone is pursuing health and longevity, the importance of food safety issues in the catering industry is self-evident. The emphasis on catering and food safety has become an important criterion for measuring the quality of life of ordinary people, the level of social management and the soundness of the country's legal system, which affects people's living standards, consumption concepts, health and social harmony and stability. As one of the important components of the public health system, catering enterprises undertake the social responsibility of ensuring the dining safety of the majority of catering consumers and the obligation to prevent catering consumers fro...

AI Technical Summary

Problems solved by technology

The current food safety inspection items are basically physical and chemical inspections and biochemical inspections. The detection indicators are mechanized and poorly related to organisms, and have defects that cannot be ignored: 1) Due to the interaction between various chemicals, the residues may also be low Chemicals that are lower than the standard value are harmful to the human body, so whether the residual amount of harmful substances below the standard value is absolutely safe for the human body, there is uncertainty

2) The existing detection system only detects known harmful substances included in the testing standards, and cannot detect harmful substances that are not listed in the testing standards or are unknown. We cannot judge and prevent possible harm caused by unknown toxic substances in food

[0009] It can be seen that the above-mentioned traditional detection methods have common technical deficiencies:

[0010] 1) Unable to detect the synergistic toxicity of different chemical substances to the human body

Due to the unknown physical and chemical properties of different chemicals, there is uncertainty about whether they can produce synergistic effects or synergistic reduction effects when interacting; it is more difficult to judge the synergistic toxicity of the samples to be tested than the toxicity of a single toxic substance

[0011] 2) Unknown additives cannot be detected, only known chemical substances can be detected

If there are analogues, derivatives of known chemical substances and their metabolites in the human body as unknown additives, it is difficult to detect

[0012] 3) There are many types of toxic substances, considering cost factors such as time, manpower, and material resources, it is impossible to cover everything and achieve full inspection

[0015] After searching the literature, it is found that the current safety evaluation of zebrafish models is all drugs and cosmetics, and there is no application in the field of catering and food.

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