33results about How to "Accelerated death" patented technology

The invention relates to an antibacterial PP material and a preparation method thereof, and belongs to the technical field of high polymer materials. The antibacterial PP material is prepared from the following components in parts by weight: 60 to 80 parts of PP resin, 2 to 4 parts of a surfactant, 0.5 to 1.5 parts of a modified antibacterial agent, 0.5 to 1.5 parts of a flame retardant and 0.2 to 0.8 part of an antioxidant, titanium ions in the modified antibacterial agent destroy bacterial cell walls, quaternized intermediate products react with proteins of cell membranes to accelerate bacterial death, and in addition, phenolic hydroxyl groups absorb ultraviolet rays with different wavelengths to inhibit the light aging process; a compact and continuous carbon layer is formed during combustion of the flame retardant and cooperates with phosphorus-oxygen double bonds and melamine, the flame retardant efficiency is improved, aluminum hydroxide coats the surface, hydroxyl is subjected to heat absorption decomposition during combustion, the surface temperature of combustible materials is stabilized, meanwhile, an inert aluminum oxide layer is formed, and the flammability is reduced. The antibacterial PP material prepared by the invention not only has good antibacterial and anti-aging effects, but also has excellent flame retardant property.

The invention provides thiamethoxam water dispersible granules and a production process thereof, and belongs to the technical field of pesticide preparation. The production process comprises the following steps: modifying methyl methacrylate; preparing an anti-ultraviolet carrier; loading eugenol; and preparing the water dispersible granules. The water dispersible granule is prepared by mixing the uvioresistant carrier loaded with eugenol with thiamethoxam, the dispersing agent, the wetting agent, the disintegrating agent and the binder, when eugenol and thiamethoxam are combined for use, the pest killing effect can be synergistically enhanced, and in addition, eugenol can bypass the resistance mechanism of pests to thiamethoxam, so that the pest killing effect is improved. Even if pests have certain tolerance to neurotoxicity of thiamethoxam, eugenol can still play a role by destroying cell membrane structures and respiratory systems of the pests, so that the resistance of the pests is broken, and the overall insecticidal effect of the prepared water dispersible granule is enhanced.

The present application relates to the technical field of nematicides for pine wood nematode disease, and specifically discloses a highly efficient compound nematicide for pine wood nematode disease and a preparation method thereof. The components of the compound nematicide include nematicides and adjuvants, the adjuvants include synergists, surfactants, solvents and rejuvenation agents, the nematicide is a mixture of emamectin benzoate and rudol, the synergist is piperonyl butoxide, the solvent includes DMF and chloride solution, and the rejuvenation agent includes amino oligosaccharide aqueous solution; in the compound nematicide, the mass fraction of rudol is 10-20%, the mass fraction of emamectin benzoate is 0.5-3.5%, and the mass fraction of the rejuvenation agent is 2-8%. The compound nematicide of the present application can also have a significant killing effect on nematodes that have developed emamectin benzoate resistance, and the selection of rejuvenation agents reduces the nematode's gnawing on pine trees, promotes the repair of internal damage to pine trees, and fully realizes the treatment of pine trees infected with pine wood nematode disease.

The invention discloses a microneedle patch loaded with a sorafenib and copper-doped Prussian blue nano material and application of the microneedle patch in in-situ triple-negative breast cancer treatment. The sorafenib and the copper-doped Prussian blue nano material are loaded in a needle body of the microneedle patch. The microneedle patch can accurately deliver nanoparticles and sorafenib to tumor tissues, the chemical kinetics effect is enhanced through an efficient and mild photothermal therapy, the ferroptosis of triple negative breast cancer cells is enhanced through ferroptosis induced by the nanoparticles and sorafenib in cooperation with drugs, then cell death is induced, and the curative effect is improved. And a brand-new and promising treatment method is provided for in-situ triple-negative breast cancer treatment.

The invention discloses a polyethylene glycol complexing agent as well as a preparation method and application thereof and belongs to the technical field of medicines. The polyethylene glycol complexing agent comprises the following components in percentage by mass: 1-2% of mesenchymal stem cell source exosome modified by miRNA-138-5p, 1-2% of mesenchymal stem cell source exosome modified by miRNA-21, 1-2% of mesenchymal stem cell source exosome modified by lncGm36569, 1-2% of mesenchymal stem cell source exosome modified by mRNA of SNARE family protein, 1-2% of mesenchymal stem cell source exosome modified by mRNA of SNARE family protein and the balance of water. 1-2% of mesenchymal stem cell source exosome modified by mRNA (messenger Ribonucleic Acid) of syntachilin, and the balance of polyethylene glycol. The polyethylene glycol complexing agent disclosed by the invention can reconstruct spinal cord continuity and improve a spinal cord injury microenvironment, so that a neurological function is recovered.

The invention belongs to the technical field of biological pharmacy, and particularly relates to application of D724-0491 in preparation of a product for preventing and treating breast cancer. The D724-0491 inhibits the combination of HBXIP and KEAP1, reduces the nuclear entry level of NRF2, further induces oxidative stress of tumor cells and promotes death of cancer cells, is a novel targeted therapeutic drug for breast cancer, and improves the prognosis survival rate of breast cancer patients.

The invention discloses an Srp54k target gene for preventing and treating tetranychus crataegus and tetranychus urticae and application of the Srp54k target gene, dsRNA synthesized by the Srp54k target gene and application of the dsRNA. The nucleotide sequences of the Srp54k target gene are shown as SEQ ID NO.1 and SEQ ID NO.2, and the nucleotide sequences of the dsRNA are shown as SEQ ID NO.3 and SEQ ID NO.4; after SRP54K is interfered, eggs of tetranychus crataegi and tetranychus urticae are caused, the survival rate is obviously influenced, and the prevention and treatment aim is achieved; the method is convenient to operate, good in effectiveness and sensitivity and high in insecticidal efficiency, has the advantages of being environmentally friendly and the like, and has a good application prospect.

The invention discloses a bionic nano drug-loaded vesicle as well as a preparation method and application thereof, and belongs to the technical field of nano preparations. The preparation method comprises the following steps: enabling a membrane-anchored functional photodynamic nano material (PACP) to interact with a breast cancer-derived cell membrane to realize embedding of a photosensitizer into a tumor cell membrane, collecting the PACP-modified cell membrane, mechanically extruding the PACP-modified cell membrane, and combining the PACP-modified cell membrane with a drug-loaded nanoparticle PLGA-DHA to obtain the bionic drug-loaded nano vesicle. Finally, the bionic photodynamic nano carrier-PACP modified tumor cell membrane vesicle with the breast cancer homing characteristic is prepared, the bionic nano drug-loaded vesicle PLGA-DHA (at) PACP-M is a photosensitizer modified cell membrane vesicle with tumor permeation / membrane fusion, and an endogenous ferroptosis signal can be activated at the same time.

The invention belongs to the technical field of biological agents, and particularly relates to application of a TDP-43 inhibitor in preparation of a medicine for treating lung adenocarcinoma. Research results show that the TDP-43 can promote lung adenocarcinoma cell proliferation and inhibit cell death. By inhibiting the expression of the TDP-43, the proliferation activity of the cells can be remarkably reduced, and the cell death rate can be improved. In addition, the TDP-43 has the function of inhibiting ferroptosis of lung adenocarcinoma cells. After the TDP-43 is silenced, the expression of the iron ion chaperonin PCBP1 is obviously reduced. The TDP-43 can be used for positively regulating and controlling the expression level of the PCBP1.

The invention discloses a TFEB acetylation site K256 and application of the TFEB acetylation site K256 in treatment of triple negative breast cancer, and belongs to the technical field of biological medicine. The acetylation site of the TFEB protein at the K256 position is identified for the first time, it is indicated that the site can serve as a treatment target of the triple negative breast cancer, and mutation of the K256 site can cause decrease of the autophagy level of cells, so that death of tumor cells is promoted. The discovery of the K256 acetylation site on the TFEB protein opens up a new path for the treatment of the triple negative breast cancer, by regulating and controlling the site, the transcriptional activity of the TFEB is enhanced, the lysosome function is promoted, and then the growth of tumor cells is inhibited, and the discovery has important significance in the aspects of early detection, illness monitoring, new drug research and development and the like of the triple negative breast cancer.

The present invention provides a nano drug for inducing ferroptosis, which is a nanoparticle formed by coordination self-assembly of an iron salt and a polyphenol drug, and a preparation method thereof comprises: dripping an organic solution containing an iron salt and a polyphenol drug into a liquid culture medium or a phosphate buffer solution to obtain a mixed solution; stirring the mixed solution to allow the iron salt and the polyphenol drug to self-assemble to obtain a nano drug. The nano drug provided by the present invention has a small size, good water solubility, and can be enriched in the tumor area, and can be used to prepare a drug for inducing ferroptosis of tumor cells. The present invention also provides a preparation method and application of the above-mentioned nano drug for inducing ferroptosis.

The utility model provides an aromatherapy application type automatic red and blue electro-microneedle instrument, which relates to the technical field of micro-needle instruments and comprises an electro-microneedle instrument body, a telescopic mechanism is arranged at the top of the inner side of the electro-microneedle instrument body, and a chassis is arranged at the bottom of the telescopic mechanism. According to the electric microneedle instrument, under the cooperation of the telescopic mechanism, regeneration and repair of stimulated skin can be facilitated through the focal dot matrix injury effect, then radio-frequency current is transmitted to the microneedles through the control of the electric microneedle instrument body, the skin absorption energy can be improved, pores can be shrunk, and through the arrangement of the red and blue light lamp beads, the skin can be effectively absorbed. Red and blue light irradiation can accelerate bacterial death through released singlet oxygen so as to achieve the effects of sterilization and inflammation diminishing, then a semi-closed liquid storage container is arranged, essential oil is stored in the semi-closed liquid storage container, small molecules in the essential oil permeate into skin, blood and particle molecules in an air fumigation mode so as to stimulate the cerebral cortex, and therefore the effects of sterilization and inflammation diminishing are achieved. The effect of relieving pain is achieved.

The invention relates to a bactericidal gynecological lotion containing composite lysozyme, and belongs to the technical field of gynecological nursing products, the gynecological lotion comprises the following raw materials: a biological membrane removal concentrated solution, a composite lysozyme concentrated solution, recombinant human collagen, a humectant, a gelatinizing agent, essence, a solubilizer, a preservative, a pH regulator and purified water. According to the invention, lysozyme and lysostaphin are compounded to form a composite lysozyme concentrated solution as an antibacterial component of the system, and meanwhile, a nonionic surfactant and a zwitterionic surfactant are compounded, so that the finished product effectively has an excellent bactericidal effect. In addition, the added biological membrane removing concentrated solution provides the capacity of disintegrating a biological membrane, an effective foundation is laid for the composite lysozyme concentrated solution, recombinant human collagen is added for nursing, and a humectant is added for improving the wettability and assisting in deep maintenance. The prepared finished product can synergistically achieve the effects of film cleaning, sterilization and maintenance.

The present invention relates to the technical field of etherifying agents, and discloses a solid cationic etherifying agent and a preparation method thereof. The present invention is carried out by adding organosilicon-modified gemini quaternary ammonium salt, pyridine-modified epoxy resin-modified quaternary ammonium salt, and 3-chloro-2-hydroxypropyltrimethylammonium chloride into a reactor for reaction, and vacuum drying is performed after the reaction is completed to obtain a solid cationic etherifying agent. In organosilicon-modified gemini quaternary ammonium salt, imidazole group inhibits the synthesis of ergosterol in fungal cell membrane, causing fungal death, and playing an antibacterial role; gemini quaternary ammonium salt has a better bactericidal effect than single-chain quaternary ammonium salt; in pyridine-modified epoxy resin-modified quaternary ammonium salt, pyridine group can interact with bacterial cell membrane, affect bacterial metabolism and cell structure, and play an antibacterial role; sulfur element can bind to protein and DNA in bacteria, inhibit enzyme activity and protein synthesis, and play a bactericidal role; sulfur element can also damage bacterial cell wall and cell membrane, accelerating cell death.

The invention discloses an mCuS (at) lm antibacterial nano cage as well as a preparation method and application of the mCuS (at) lm antibacterial nano cage. The mCuS (at) lm antibacterial nano cage takes hollow mesoporous nano copper sulfide nanoparticles as a core, a bacterial copper death amplifying agent and a cell copper death inhibitor are entrapped in the hollow mesoporous nano copper sulfide nanoparticles through electrostatic adsorption self-assembly, and the surfaces of the hollow mesoporous nano copper sulfide nanoparticles are further coated with bionic cell membranes, so that the mCuS (at) lm antibacterial nano cage is obtained. The nanometer cage is the mCuS (at) lm antibacterial nanometer cage. The mCuS (at) lm antibacterial nano cage surface biomimetic membrane highly expresses proteins related to microbial recognition and uptake, can effectively neutralize inflammatory factors in the environment, and has a potential infection targeting effect. The bacterial copper death amplifying agent is used for enhancing bacterial copper death, and the cell copper death inhibitor is used for retarding host cell copper death, so that the bacterial killing effect and the antibacterial effect are improved under the condition that the biological safety is improved.

The invention belongs to the technical field of fiber modification, and provides a chitosan modified nylon fiber as well as a preparation method and application thereof. The preparation method comprises the following steps: mixing chitosan with an acid solvent to obtain a chitosan solution; the preparation method comprises the following steps: soaking nylon fibers in a chitosan solution, and sequentially carrying out drying treatment and curing treatment after soaking is finished, so as to obtain the chitosan modified nylon fibers. Wherein the chitosan is a natural polysaccharide, is non-toxic and harmless and meets the environmental protection requirement, and meanwhile, harmful chemical substances are not used in the preparation process, so that the safety of the product is ensured; the chitosan modified nylon fiber has a remarkable antibacterial effect, and the chitosan coating on the surface of the nylon fiber can effectively inhibit growth and reproduction of various bacteria and fungi. The chitosan modified nylon fiber prepared by the invention can be widely applied to the fields of medical supply packaging or food packaging and the like, and has great market potential.

The invention discloses an application of an epigenetic drug combined with adoptive monocyte / macrophage immunotherapy in the preparation of a biopharmaceutical for tumor diseases, belonging to the technical field of biopharmaceuticals. The present invention provides an application of an epigenetic drug combined with adoptive monocyte / macrophage immunotherapy in the preparation of a biopharmaceutical for tumor diseases, wherein the epigenetic drug removes the methylation of tumor cells, increases the susceptibility of tumors to targeted therapy and regulates tumor heterogeneity, and the chimeric antigen receptor extracellular domain of the CAR monocyte / macrophage targets and recognizes and blocks the signal pathway of targeted tumor antigens, and performs cellular immune killing. The epigenetic modification of the present invention and CAR monocyte / macrophage anti-solid tumor treatment have a synergistic therapeutic effect, and the epigenetic modification treatment makes tumor antigens more sensitive to CAR monocyte / macrophage therapy, promotes the expression of tumor suppressor genes, promotes the conversion of tumor cell apoptosis to pyroptosis, and solves the problem of immune escape tolerance caused by tumor heterogeneity.

The present invention belongs to the field of preparation of longan ginseng peptide probiotic powder, and particularly relates to a traditional Chinese medicine fermented longan ginseng peptide probiotic powder for treating tumors. Ginseng and American ginseng are crushed and powdered, vacuum concentrated to make ginseng peptide concentrate, and mixed with dried longan, artemisia annua, ashwagandha, knotweed, tripterygium wilfordii, cordyceps sinensis, and composite probiotic agents in a fermenter for full fermentation. The effective ingredient content is high and is conducive to absorption, while clearing away heat and promoting body fluid without toxic side effects; finally, the traditional Chinese medicine fermented longan ginseng peptide probiotic powder prepared by vacuum freeze drying has minimal biological activity damage, completely retains the active ingredients for treating tumors, and is convenient for long-term storage. After liver cancer mice take it, the tumor weight and volume are effectively suppressed, the liver function is less damaged, and the immune function of mice is protected, and the macrophage phagocytosis rate of mice reaches 79.6%.

The application belongs to the field of biological pesticides, and provides application of HearNPV-miR-56 in cotton bollworm control, wherein the nucleotide sequence of the HearNPV-miR-56 is CGGUAACAGAAGCGGUCACG. The HearNPV-miR-56 can improve virus replication amount of cotton bollworm nuclear polyhedrosis virus infected larvae, thereby promoting death, and can be used for developing a cotton bollworm nuclear polyhedrosis virus synergist, and has potential application prospect in biological control of cotton bollworm.

The structural formula of the small molecule compound for inhibiting PINK1 is # imgabs0 #, R1 is CH3 or # imgabs1 #, and R2 is # imgabs2 #. The small molecule compound provided by the invention has an obvious inhibiting effect on PINK1, and by inhibiting the activity of PINK1 kinase, the phosphorylation level of Ub-Ser65 is obviously reduced, and mitochondrial damage accumulation is promoted, so that tumor cell proliferation is inhibited. The small molecule compound has an excellent anti-tumor effect and has a remarkable effect of inhibiting cell proliferation on liver cancer cells.

This application discloses the use of USP15 inhibitors in the preparation of medicaments for the treatment and / or prevention of renal cell carcinoma, as well as pharmaceutical compositions and kits thereof.

This invention provides a method for preparing a scale-inhibiting and bactericidal water treatment material, belonging to the technical field of water treatment materials. The method includes the following steps: adding PSI and water and stirring to disperse; adding L-histidine and NaOH; heating and reacting to obtain a histidine-modified polyaspartic acid solution; adjusting the pH to neutral; adding ferric ammonium citrate and ascorbic acid; stirring and reacting to obtain an iron-containing complex mixed solution; dissolving β-cyclodextrin in water; adding succinic anhydride and reacting; then adding an ε-polylysine aqueous solution; using EDC and NHS as condensing agents; stirring and reacting; dialysis and filtration to obtain a polylysine-cyclodextrin complex solution; adding the polylysine-cyclodextrin complex solution dropwise to the iron-containing complex mixed solution; ultrasonically stirring; adding sodium citrate and molten PEG-6000; heating and mixing; extruding; and drying to obtain the finished product. The scale-inhibiting and bactericidal water treatment material obtained by this invention can achieve efficient scale inhibition while also possessing bactericidal function, and its components are safe and environmentally friendly.

The invention provides a composite algae removal material. The composite algae removal material is composed of montmorillonite-loaded chitosan nanowires and polyethylene glycol grafted Ag-loaded GO / CNC microspheres, the preparation method comprises the following steps: respectively mixing montmorillonite-loaded chitosan nanowires and polyethylene glycol grafted Ag-loaded GO / CNC microspheres with water, preparing a montmorillonite-loaded chitosan nanowire solution and a polyethylene glycol grafted Ag-loaded GO / CNC microsphere solution, and sequentially spraying the two solutions on the surface of algae to remove the algae. The algae removal material is composed of montmorillonite-loaded chitosan nanowires and polyethylene glycol grafted Ag-loaded GO / CNC microspheres, the two algae removal materials are sequentially added into an algae-containing water body, and the algae removal effect is improved through the synergistic effect.

The application discloses a soybean-corn compound planting intelligent weeding robot, and relates to the technical field of agricultural robots. The traction increasing mechanism can be used for synchronously adjusting the intervals of the multiple weeding mechanisms by the interval regulation assembly, so that the intervals of the multiple weeding mechanisms can be flexibly adjusted under different planting plant intervals of corn and soybean, the rapid weeding operation under the compound planting mode is improved, and the weeding efficiency is improved. The cultivation mechanism can collect, cut and stir the weeds and part of the soil which are dug by the weeding mechanism. The cutting process can damage the rhizome part of the weeds, so that the weeds lose the regenerative ability, the weeds after being dug are prevented from regenerating under suitable conditions, and the effect of completely weeding is achieved. The stirring process can mix the cut weeds and the soil, the weeds can be decomposed by the microorganisms in the soil after being mixed in the soil, so that the abandoned weeds can form nutrient substances which are beneficial to the growth of soybean and corn.

The invention belongs to the technical field of agricultural biology, and particularly relates to a nucleic acid pesticide for preventing and controlling tetranychus urticae and application of the nucleic acid pesticide. Research finds that after the expression quantity of the Pb1 gene of the tetranychus urticae is reduced by an RNA interference technology, the growth and development abnormity and the death rate of the tetranychus urticae are obviously improved, the medial lethal concentration of the tetranychus urticae is 4.86 ng / mu L, and the prevention and control effects on eggs, larvae and adults are 92.76%, 84.24% and 96.14% respectively when the double-stranded RNA of Pb1 is sprayed on the surfaces of plants, so that the application of the Pb1 gene of the tetranychus urticae is promoted, and the application of the Pb1 gene of the tetranychus urticae is promoted. Therefore, the target gene has great potential in the prevention and control of the tetranychus urticae, and meanwhile, a new way is provided for the prevention and control of the tetranychus urticae by utilizing the double-stranded RNA.

The invention discloses a TuHel31B target gene for preventing and treating tetranychus urticae and application of the TuHel31B target gene, dsRNA synthesized by the TuHel31B target gene and application of the dsRNA. The nucleotide sequence of the TuHel31B target gene is as shown in SEQ ID NO. 1, and the nucleotide sequence of the dsRNA is as shown in SEQ ID NO. 2; after the TuHel31B is interfered, the egg laying amount of the tetranychus urticae is obviously reduced, and the survival rate is obviously influenced due to abnormal growth and development, so that the prevention and treatment purpose is achieved; the method is convenient to operate, good in effectiveness and sensitivity and high in insecticidal efficiency, has the advantages of being environmentally friendly and the like, and has a good application prospect.

The invention discloses a micelle mixing system for enhancing the treatment effect of irreversible electroporation on pancreatic cancer and a preparation method of the micelle mixing system, the injectable micelle mixing system is formed by mixing CGP37157-loaded PEG-PCL micelle CGP37157-coated PEG-PCL and a CaCl2 solution, and a CGP37157 sodium-calcium exchange inhibitor which is insoluble in water can be delivered to tumor cells; during electroporation treatment, calcium ions in mitochondria are inhibited from being discharged along with increase of permeability of tumor cells, calcium overload of the mitochondria is further caused, death of the tumor cells is accelerated, the efficiency of IRE treatment can be effectively improved, and good clinical application prospects are achieved.

The invention relates to a tumor microenvironment response type nano assembly as well as a preparation method and application thereof, and belongs to the technical fields of pharmaceutical preparation combined treatment, new auxiliary materials and new dosage forms. The invention discloses a tumor microenvironment response type nano assembly which is formed by co-assembling DPA-MOF nanoparticles, manganese dioxide and a porphyrin photosensitizer through intermolecular force and is modified by adopting a PEG (Polyethylene Glycol) modifier. Wherein the mass ratio of the DPA-MOF nanoparticles to the manganese dioxide to the porphyrin photosensitizer is 1: (0.1-10): (0.1-15), and the mass ratio of the sum of the DPA-MOF nanoparticles, the manganese dioxide and the porphyrin photosensitizer to the PEG modifier is 1: (0.1-9). The nano assembly device has sensitive < 1 > O2 self-reporting capability, improves tumor hypoxic microenvironment capability and exhausts GSH in tumor cells, further has a good anti-tumor effect and relatively high safety, and provides a new strategy for synergistic combination of a singlet oxygen detection probe and other drugs.

The invention belongs to the technical field of biological medicines, and particularly relates to application of SIRT5inhibitor 1 in treatment of Philadelphia chromosome positive acute lymphocytic leukemia. The invention proves that the SIRT5inhibitor 1 can be used for specifically inhibiting the expression of the SIRT5 of the Ph + ALL cell; it is found for the first time that the SIRT5inhibitor 1 can weaken the Ph + ALL cell proliferation capacity, the cell cycle is retarded in the G2 / M period, and the apoptosis level is remarkably increased; it is found that the SIRT5inhibitor 1 enables the mitochondrial membrane potential level of the Ph + ALL cells to be obviously reduced, respiratory chain electron leakage is caused, reactive oxygen species (ROS) in the cells is increased, and then cell death is promoted; when the NAC is used for resisting the ROS effect, the effect of the SIRT5inhibitor 1 for causing apoptosis of the Ph + ALL cells is reversed, and it is further proved that the SIRT5inhibitor 1 promotes cell death by increasing the ROS in the Ph + ALL cells. The invention aims to provide a new strategy and method for treating Ph + ALL.

The invention discloses application of CD317 siRNA combined with an AMPK inhibitor or AMPKsiRNA in tumor treatment, and belongs to the technical field of biological medicines. The invention provides application of CD317 siRNA (small interfering Ribonucleic Acid) or combination of a CD317 inhibitor and an AMPK (adenosine monophosphate kinase) inhibitor or AMPKsiRNA in preparation of a medicine for treating tumor diseases. The CD317 siRNA or the CD317 inhibitor is used for inhibiting the expression of CD317 through gene silencing or gene editing, or is used for degrading CD317 protein in a targeted manner through PROTAC and LYTAC technologies. According to the invention, the expression of cancer cells CD317 is reduced through the CD317 siRNA or the CD317 inhibitor, so that AMPK signals are inhibited, and the sensitivity of tumor cells to the AMPK inhibitor is enhanced. The composition of the CD317 inhibitor and the AMPK inhibitor can remarkably promote death of tumor cells, and a novel anti-tumor method is provided.