30results about How to "Good killing effect" patented technology

This invention discloses a portable low-temperature plasma device based on a Laval nozzle structure and its application, belonging to the field of plasma technology application technology. Specifically, it includes (1) the construction of the device; and (2) the use of the device for fungal eradication treatment and transdermal drug delivery enhancement treatment. The beneficial effects achieved by this invention are as follows: This invention is the first to apply the Laval nozzle structure to a portable low-temperature plasma device. The results show that the device can effectively improve the plasma jet performance and active particle content, significantly improve the treatment effect of fungal infections of the skin and nails and the transdermal drug penetration efficiency, while solving the problems of large size, reliance on external power supply, and complex operation of traditional plasma devices. Therefore, using this invention for the treatment of fungal infections of the skin and nails and for enhanced transdermal drug delivery has advantages such as ease of operation, high safety, non-invasiveness, and high efficiency, and thus has potential application prospects in the field of skin disease treatment and transdermal drug delivery.

This application belongs to the field of microbial technology, specifically relating to the application of a strain of Metarhizium anisopliae JXASMS-01 and its extract in pest control. This Metarhizium anisopliae (… Metarhizium The extract of *Metarhizium anisopliae* sp. JXASMS-01 was deposited on June 8, 2022, at the China Center for Type Culture Collection (CCTCC), No. 299, Bayi Road, Wuchang District, Wuhan, Hubei Province, China, with accession number CCTCC NO: M 2022833. This extract exhibits good killing effects against cotton bollworms and beet armyworms, without the drawbacks of chemical pesticides, enabling sustainable control of lepidopteran pests.

An antibody-drug conjugate, comprising a bispecific antibody targeting HER2 or an antigen-binding fragment thereof. Also provided are a preparation method for and an application of the antibody-drug conjugate, and a pharmaceutical composition comprising the antibody-drug conjugate. The antibody-drug conjugate can effectively kill tumors.

The invention provides a tumor diagnosis and treatment agent with a targeting function and a preparation method thereof, and belongs to the field of biomedicine. According to the method, triphenylamine is taken as a starting unit, a pi conjugated system is expanded through a benzothiadiazole group, pyridinium salt is introduced as a strong electron withdrawing unit, alkyl chains with different lengths are connected into molecules, and finally, diagnosis and treatment integrated molecules with fluorescence imaging and tumor targeting functions are synthesized. The fluorescent targeted diagnosis and treatment agent not only has good tumor cell plasma membrane targeting accuracy, but also has excellent aggregation-induced emission performance which is obviously higher than that of a traditional commercial photosensitizer, so that accurate targeted localization of tumor cells is realized. Meanwhile, the fluorescent targeting diagnosis and treatment agent TBPPM has excellent singlet oxygen generation capability, and further shows relatively strong tumor cell killing capability. Therefore, the prepared fluorescent targeted diagnosis and treatment agent provides a new material for accurate positioning and treatment of tumors under fluorescence guidance, and also provides a new thought for development of targeted diagnosis and treatment agents for breast cancer.

The application relates to the technical field of fibers, and discloses a long-acting antibacterial fiber and a preparation process thereof. 1,3,5-tri(methyl acetate trimethyl ammonium bromide) benzene and ethylenediamine are subjected to amidation condensation reaction to obtain a quaternary ammonium salt antibacterial agent, then electrostatic spinning is carried out with polyurethane resin to obtain the long-acting antibacterial fiber. The antibacterial agent contains a large number of positive charges, can destroy the negatively charged cell membranes of bacteria, inhibit the growth of bacteria, has a good killing effect, and significantly improves the long-acting antibacterial performance of the fiber. The quaternary ammonium salt antibacterial agent contains a large number of amide bonds, forms hydrogen bond force with urethane groups of the polyurethane, has good compatibility, has little influence on the mechanical properties of the fiber, the polyurethane fiber maintains good breaking strength and breaking elongation, and can have good mechanical properties and antibacterial performance.

The application discloses an mRNA drug for enhancing T cell efficacy and application, and belongs to the technical field of biological medicine. The mRNA drug encodes a protein, which is a BH3 domain of pro-apoptotic protein Puma or Bim fused with an aptamer scaffold; and the administration mode is an intratumoral administration dosage form. The application provides an mRNA anti-tumor drug, which is locally administered intratumorally in the form of the mRNA drug, induces local cell apoptosis of a lesion, and can synergistically enhance T cell-mediated killing effect. Finally, the effect of synergistically inhibiting growth of a solid tumor in vivo is achieved. The novel mRNA drug provided by the application exhibits a strong and effective tumor inhibition effect in a solid tumor model.

This invention relates to the field of wastewater treatment and discloses a device and method for the combined removal of heavy metals and antibiotics from wastewater. The device includes: a coagulation reaction zone, a pre-sedimentation tank, a high-efficiency sedimentation tank, an ozone distribution zone, an ozone oxidation tank, a primary anoxic tank, a primary aerobic tank, a secondary anoxic tank, a secondary aerobic tank, and a disinfection tank. The coagulation reaction zone is symmetrically and fixedly connected to one side of the pre-sedimentation tank, and the high-efficiency sedimentation tank is fixedly connected to the other side of the pre-sedimentation tank. This invention achieves rapid and efficient sedimentation of coagulated flocs through the combination of vertical and horizontal flow high-efficiency sedimentation tanks, removing heavy metals from wastewater in the form of precipitates. Furthermore, the advanced ozone oxidation process removes antibiotics, improves the biodegradability of the wastewater, and simultaneously disinfects the effluent, saving investment. In addition, the efficient solid-liquid separation of flat sheet membrane modules replaces the secondary sedimentation tank, effectively ensuring that the effluent is clear and meets standards.

This invention belongs to the field of biomedical technology and discloses the use of fluoropyridoxal in the preparation of drugs for anticancer purposes, including: use in drugs for the prevention and / or alleviation and / or treatment of cancer. The cancer is ovarian cancer. The drug for the prevention and / or alleviation and / or treatment of cancer comprises fluoropyridoxal and a pharmaceutically acceptable carrier. The dosage form of the drug for the prevention and / or alleviation and / or treatment of cancer is tablets, granules, capsules, suspensions, syrups, emulsions, or injections. Fluoropyridoxal induces senescence-like death, cell cycle arrest, and apoptosis in cancer cells. Fluoropyridoxal acts by inducing apoptosis in cancer cells. The fluoropyridoxal of this invention can effectively inhibit the proliferation and growth of human ovarian cancer cell lines SKOV3, OVCAR3, 3AO, and ES-2. The fluoropyridoxal of this invention can be used as an effective drug for the treatment of ovarian cancer.

The utility model relates to a kind of tea fixation device, the process of rotation of push pin (20) can contact with dial (17), to make dial (17) move to the direction of far from roller (11), drive baffle (14) move to make spring (15) be compressed, when push pin (20) and dial (17) are disengaged, baffle (14) reciprocatingly slide along the inner wall of roller (11) under the action of spring (15) until stop, during the volume variation of the inner cavity of roller (11), so that hot air flow in roller (11) generates transverse vibration and acts on tea, it is realized in the rotation of roller (11) and makes tea leaf turn over, tea leaf can collide each other under the action of hot air flow, avoid tea leaf shrink into tea ball due to heat accumulation, and fully contact with hot air flow, so that tea leaf is dried, to improve the uniformity and fixation effect of tea fixation, avoid affecting subsequent processing and the quality of finished tea leaf.

The present application relates to the technical field of biological medicine, in particular to a kind of nano-molecular probe with targeting and its preparation method and application.The plasmid of immune checkpoint corresponding receptor of stable expression tumor surface negative regulation molecule is synthesized in the present application, which is prepared into targeted nano-molecular probe with cell membrane nanocapsule of enhancing T cell activity ligand and being marked with fluorescence, and melanin nanosphere of simultaneously loading therapeutic drug.The targeted nano-molecular probe provided in the present application can be long-circulated in vivo, reduce the dosage and administration frequency of chemotherapeutic drug.It also has the advantages of improving the drug resistance of tumor site to therapeutic drug, targeting tumor DNA damage and significantly enhancing the infiltration and activation of immune cells in tumor site.

The utility model relates to the technical field of tea processing, and discloses a drum-type tea fixation device for tea making, which comprises a support frame, a heat preservation layer, a heating drum, a material turning mechanism, a driving shaft, a hollow groove, an air blowing pipe, a support arm, an air box, a mounting groove and a motor, according to the tea leaf frying machine, after tea leaves enter the heating cylinder, the heating cylinder can heat and stir-fry the tea leaves through the material turning mechanism when rotating, when the motor is started, the motor can drive the air blowing pipe outside the driving shaft to rotate through the driving belt, and the tea leaves are heated and fried through the air blowing pipe. After the air delivery pipe delivers hot air into the air box, the hot air can be delivered to the air blowing pipe through the driving shaft, so that the stir-fried tea leaves in the heating cylinder are circularly blown, the dispersion of the tea leaves is accelerated, the tea leaves can be uniformly heated, and the tea leaf fixation effect is improved.

The utility model is suitable for the technical field of tea leaf processing, and provides a tea leaf fixation device which comprises a base, the support frame is fixed at the top of the base; the cylinder body is fixed on the support frame and is used for supporting tea leaves to be subjected to enzyme deactivation processing; an opening for providing a tea leaf inlet and outlet channel is formed in the cylinder body; the first protective cover and the second protective cover are hinged to the barrel, and the first protective cover is matched with the second protective cover to be used for adjusting the size of the opening; the hopper braising turning plate is rotationally mounted in the barrel body and is used for turning tea leaves; and the servo motor is fixed on the support frame and is used for driving the bucket stuffiness turning plate to turn. According to the tea leaf fixation device provided by the scheme, feeding and discharging are convenient, heat loss can be reduced to the maximum extent in the fixation process so as to reduce energy waste, meanwhile, braising and throwing can be combined, and the fragrance of tea leaves is reserved.

The application provides a high-efficiency expansion culture method of umbilical cord blood gamma delta T cells and application thereof, and belongs to the technical field of biological medicine. Through a specific two-stage strategy of "initial induction culture + expansion culture", the application is simple in operation, stable in system, and high in success rate, effectively overcomes the defects of limited autologous blood source, unstable expansion and low cell activity in the prior art, and lays a foundation for large-scale preparation and allogeneic application of gamma delta T cells. The umbilical cord blood gamma delta T cells obtained by the method of the application are excellent in quantity, purity, activity and anti-tumor function, and have stronger tumor cell killing ability, and are especially suitable for adoptive immunotherapy. The application breaks through the technical bottleneck of the traditional culture system, provides a high-efficiency, stable and clinically convertible gamma delta T cell expansion scheme, and has important application prospect and market value.

The invention discloses an application of a bacterial strain in production of an external product for improving the micro-ecology of the vagina of a human body, and the bacterial strain is Lactobacillus crispatus SPR003 and has 16S rDNA (ribosomal deoxyribonucleic acid) as shown in SEQ ID NO.1. The invention further discloses a preparation method of the bacterial strain for improving the micro-ecology of the vagina of the human body. The preservation number is GDMCC NO: 66664; the strain produces H2O2 and only produces D-lactic acid, and no drug-resistant gene is found; the external product is suitable for improving changes, caused by vaginal pathogenic bacteria, of micro-ecology of the vagina of the human body. Compared with other lactobacillus crispatus of the same type, the strain has the advantages that the H2O2 production capacity is high, the H2O2 production speed is high, the total amount of H2O2 produced within 12 hours is large, and the concentration of hydrogen peroxide can reach about 220 mu M / L after the strain is cultured for 12 hours in the conventional inoculation amount of 1%. H2O2 is a strong oxidant, and can directly kill or inhibit a plurality of pathogenic bacteria and conditioned pathogenic bacteria such as Gardner bacteria, campylobacter actinomycetes, prevotella and other BV-related anaerobic bacteria.

The utility model discloses a kind of green tea killing green automatic stir-frying devices, belong to tea processing equipment technical field, a kind of green tea killing green automatic stir-frying device, including shell, with the shell rotation connection's drum and install in the lower portion of the drum for supporting the rack of the shell, the bottom end of the rack is fixedly connected with the edge portion of the drum terminal end near support, the bottom of the support is welded with the support fixed connection of through bolt bracket, driving motor is installed on the bracket, the power output end of the driving motor is installed with gear, the terminal outer wall of the drum is installed with the gear ring engaged with the gear, the port inner wall of the drum is fixedly connected with the connecting frame symmetrically, shaft rod is rotatably connected between the connecting frame and the inner end wall of the drum through bearing, it not only can stir-fry more tea leaves at one time, and stir-fry evenly, make tea leaves can be evenly heated, improve the effect of tea killing green.

The invention discloses an application of a bacterial strain in production of an external product for improving the micro-ecology of the vagina of a human body, and the bacterial strain is Lactobacillus crispatus SPR002 and has 16S rDNA (ribosomal deoxyribonucleic acid) as shown in SEQ ID NO.1. The invention also discloses an application of the bacterial strain in production of the external product for improving the micro-ecology of the vagina of the human body. The strain produces H2O2 and only produces D-lactic acid, and no drug-resistant gene is found; the external product is suitable for improving changes, caused by vaginal pathogenic bacteria, of micro-ecology of the vagina of the human body. When the strain concentration is 1 * 10 < 7 > CFU / mL, the strain SPR002 provided by the invention can generate a strong bacteriostatic effect on staphylococcus aureus, and the bacteriostatic rate reaches 98.15%; the bacteriostasis rate of the bacterial strain SPR002 to candida albicans can be obviously improved along with the increase of the concentration of the bacterial strain SPR002, and when the concentration of the bacterial strain is 3 * 10 < 8 > CFU / mL, the bacteriostasis rate of the bacterial strain SPR002 to candida albicans reaches 97.1%. Compared with other Lactobacillus crispatus of the same type, the strain SPR002 provided by the invention can generate a relatively strong inhibition effect on Candida albicans along with the increase of the concentration, so that the strain SPR002 provided by the invention can be developed into a medicine product, and the dosage of the medicine is appropriately increased according to the condition of a patient.

The application discloses a doxorubicin-quadruple platinum drug-norcantharidin three-drug combination nano drug and a preparation method and application thereof, and belongs to the technical field of medical materials. 2+ The application constructs a novel nano drug through coordination of doxorubicin and a quadruple platinum drug modified by norcantharidin, and then polymerizes polydopamine with a mild photothermal effect to prepare a nano drug for the three-drug combination of doxorubicin, the quadruple platinum drug and norcantharidin. The nano drug has the ability of controlled release of drugs under acidity and reduction, can realize photothermal conversion to generate a mild photothermal effect under laser irradiation, has a better tumor cell killing effect, has the ability of consumption of glutathione and generation of hydroxyl radicals, can enter cancer cells through an endocytosis mode and release DOX, and provides a new idea for multi-drug combination, chemotherapy-chemical kinetics treatment-photothermal treatment multi-mode combined treatment.

The invention relates to the technical field of daily chemicals, in particular to an antibacterial linen detergent which comprises a surfactant, a builder, a unique composite antibacterial system and a composite biological enzyme preparation. The composite antibacterial system is formed by compounding a double-chain quaternary ammonium salt, a guanidine polymer and a plant-source antibacterial synergist according to a specific proportion, the composite antibacterial system and a biological enzyme preparation containing protease, lipase, amylase and cellulase generate a synergistic effect, and through a mechanism of first enzymolysis cleaning and then multi-target synergistic sterilization, the composite antibacterial system can be used for preparing a multi-target antibacterial agent under the low-temperature condition of 40-60 DEG C; the efficient decontamination on the linen and the broad-spectrum and lasting killing on bacteria and fungi can be realized, and the drug resistance risk of microorganisms is obviously reduced. The product does not contain a chlorine bleaching agent, is environment-friendly and safe, has small damage to fabrics, and is particularly suitable for hygienic washing of linen in the industries of hotels, hospitals and the like.

The invention belongs to the technical field of traditional Chinese medicine nursing, and discloses a traditional Chinese medicine health-care comb and a preparation method thereof.The traditional Chinese medicine health-care comb is prepared through the steps that traditional Chinese medicine powder, nanmu sticky powder, a nanometer whisker reinforcing agent and composite antibacterial factors are subjected to dry mixing and then mixed with a traditional Chinese medicine extracting solution according to the mass ratio of 1: 1, and compression molding is conducted; the mass ratio of the traditional Chinese medicine powder to the nanmu sticky powder to the nano whisker reinforcing agent to the composite antibacterial factor is (65-70): (20-25): (5-8): (2-3); through a double-effect fusion mode of the medicine powder and the extracting solution, combination of physical massage and medicine effect permeation is achieved, compatibility of multiple medicinal materials is achieved, the traditional Chinese medicine theory and scalp care requirements are considered, and multiple health-care effects of nourishing blood and blacking hair, growing hair and preventing hair loss, promoting blood circulation to remove meridian obstruction and balancing the scalp environment are achieved; meanwhile, a nano whisker reinforcing agent and a composite antibacterial factor are also added, so that the problems that the traditional Chinese medicine health-care comb is easy to break and mildew and short in service life in life and use are solved.

The invention discloses a long-acting antibacterial textile fabric and a preparation method thereof, and belongs to the technical field of functional after-finishing of textile materials and textiles. The long-acting antibacterial textile fabric comprises a base cloth layer and an antibacterial functional layer attached to the surface of the base cloth layer and in fiber gaps, the antibacterial functional layer is prepared from the following components in parts by weight: 1 to 15 parts of chitosan antibacterial organic composite nano silver oxide composite particles, 1 to 8 parts of polyhexamethylene biguanide hydrochloride, 0.5 to 5 parts of a silane coupling agent, 10 to 40 parts of a waterborne polyurethane adhesive, 0.5 to 4 parts of a nano titanium dioxide photocatalyst and 40 to 100 parts of deionized water. According to the invention, the specific organic antibacterial component is introduced to have a synergistic effect with chitosan, so that the problem that inorganic nanoparticles are easy to agglomerate is effectively solved.

The utility model discloses fixation equipment for green tea production, which belongs to the technical field of fixation equipment and comprises a bottom plate, a fixation cylinder and two vertical plates, and the fixation cylinder is rotatably mounted between the two vertical plates; a hydraulic cylinder is rotatably mounted on the top surface of the bottom plate, mounting plates are symmetrically and fixedly mounted at the bottom of the fixation barrel, and the telescopic end of the hydraulic cylinder is rotatably mounted between the two mounting plates; a cross is fixedly mounted on the inner wall of an opening of the fixation cylinder, a motor is fixedly mounted on the side wall of the cross, a rotating rod is fixedly mounted at the tail end of an output shaft of the motor, mounting sleeves symmetrically and fixedly sleeve the side wall of the rotating rod, a plurality of limiting cylinders are correspondingly and fixedly mounted on the side walls of the two mounting sleeves, and telescopic rods are slidably mounted in the limiting cylinders; and a scraping plate is fixedly mounted at the tail end of the telescopic rod. Compared with the prior art, the device can prevent green tea from being damaged during stir-frying and fixation, and also can discharge tea leaves after fixation.

This invention discloses a medical material for the prevention and treatment of oral ulcers, its preparation method, and its application. The medical material of this invention comprises a two-dimensional sheet-like nanomaterial composed of an organic matrix and metallic elements. The two-dimensional sheet-like nanomaterial of this invention possesses advantages such as high crystallinity, favorable material transport, high healing rate, reduced recurrence risk, excellent biocompatibility, mild preparation process, scalability, ease of use, and low cost, and has broad application prospects in the field of oral ulcer prevention and treatment.

The invention relates to a long-acting antibacterial and anti-algae waterproof coating for a drinking water pool, the long-acting antibacterial and anti-algae waterproof coating for the drinking water pool comprises a liquid material and a powder material, the liquid material comprises an organic silicon emulsion, modified organic silicon, color paste, an anti-algae agent, an antibacterial agent, an auxiliary agent and water, and the powder material comprises an organic silicon emulsion, modified organic silicon, color paste, an anti-algae agent, an anti-bacterial agent, an auxiliary agent and water. The powder comprises white cement, quartz sand, PP fibers, cellulose and a water reducing agent. On the basis of compounding modified organic silicon obtained by reacting a reaction product of vinyl hydrogen-containing silicone oil and allyl glycidyl ether modified chitosan with N, N-methylene bisacrylamide, styrene and butyl acrylate with an organic silicon emulsion, an antibacterial agent, an anti-algae agent and an auxiliary agent are matched, so that antibacterial and anti-algae functions and system stability are realized; the powder is cooperated with the quartz sand through the white cement, a ventilation channel is constructed by means of the quartz sand, the waterproof performance is guaranteed through the cement, a three-dimensional toughening network is formed through the PP fibers and the cellulose, and the constructability and the coating compactness are optimized in cooperation with the water reducing agent.

The application relates to an aryl boron compound, a preparation method and application thereof, and a pharmaceutical composition, which is used for tumor imaging, has high fluorescence quantum efficiency through intramolecular charge transfer, and can be connected to different antibodies through Q3 under a certain concentration, and can accurately target corresponding tumors according to different antibodies. The boron drug preparation can be used for boron neutron capture therapy to kill tumor cells. The compound has a better killing effect, and the compound is targeted to tumor cells through antigen-antibody interaction. After the boron-10 drug is gathered at a tumor site, neutron radiation is generated, a nuclear reaction is triggered to release alpha particles and 7 Li particles kill cells, and the ranges of the two particles are about 10 mu m. High-energy-density heavy ions break the double helix structure of DNA in tumor cells, so that the cells cannot be repaired and die completely, and the distance of 10 mu m avoids damage to normal cells and tissues.

The utility model discloses a vertical cabinet type sophora flower bud de-enzyming device which comprises a steam boiler and is characterized by further comprising a vertical cabinet type de-enzyming bin with a bin door, the vertical cabinet type de-enzyming bin is communicated with the steam boiler through a pipeline, and a steam pipeline which is provided with steam holes and is communicated with the pipeline is arranged on the inner bottom wall of the vertical cabinet type de-enzyming bin. A cart type loading frame is arranged in the vertical cabinet type fixation bin, the cart type loading frame is provided with a door facilitating loading / unloading, a partition frame is arranged in the middle of the cart type loading frame, and during fixation, sophora flower bud ears are loaded into the vertical cabinet type fixation bin through the cart type loading frame for fixation. The device is helpful for loading / unloading sophora flower bud ears at a position which is far away from the fixation bin by a certain distance. In addition, due to the special structural arrangement of the cart type loading frame, feeding and unloading are facilitated, and sophora flower bud ears can be uniformly heated, so that the fixation effect is improved.

The invention provides miRNA miR-148b-3p for targeted regulation of the action of SLC7A11 in ferroptosis as well as an action mechanism and application of the miRNA miR-148b-3p, and finds and proves that miR-148b-3p can promote the ferroptosis process of breast cancer cells and inhibit proliferation and migration of the breast cancer cells through targeted regulation of the SLC7A11 for the first time. Related action mechanisms are disclosed by researches such as construction of a ceRNA network, and potential targets and strategies are provided for breast cancer treatment. Meanwhile, it is found that the compound also has a regulation effect in other cancers, but the effect is weaker than that of the breast cancer, the specific regulation effect on the breast cancer is highlighted, and a solid theoretical foundation is laid for accurate treatment of the breast cancer, so that breast cancer treatment and research are more effectively carried out. Meanwhile, regulation and control of the miR-148b-3p on ferroptosis are researched through various analyses, and it is shown that the miR-148b-3p can regulate ferroptosis related genes, and then cell survival and death are regulated and controlled. A new perspective is provided for breast cancer treatment, a basis is also provided for research on tumor cell biological behaviors, and the application prospect is important.

The application relates to the field of biochemical technology, in particular to a protein-BODIPY photosensitizer co-assembled nano reagent and a preparation method and application thereof. The BODIPY photosensitizer structure is as follows: the BODIPY photosensitizer is co-assembled with BSA to obtain a nano reagent, the nano reagent has very excellent optical performance, irradiation is carried out under very low power density, has very high singlet oxygen generation efficiency, in-vitro the nano reagent all shows good cancer cell killing effect, and has very high biological safety, and has extremely considerable application prospects in the biomedical field.

The invention relates to the technical field of epoxy paint, in particular to mildew-proof epoxy paint for high-temperature and high-humidity environments and a preparation method thereof.The mildew-proof epoxy paint is prepared from epoxy resin, a curing agent, a mildew-proof agent formed by compounding a nano-silver antibacterial agent and MIT, pigment filler and auxiliaries; the preparation method comprises the steps of premixing, pigment and filler dispersion, mildew preventive mixing, defoaming, rheological modification, blending and the like, and through a specifically compounded mildew preventive system, the problems that in the prior art, a single mildew preventive is prone to losing efficacy in a high-temperature and high-humidity environment, and the mildew-proof durability is poor are solved; meanwhile, the comprehensive performance such as excellent adhesive force, hardness and impact resistance of a paint film is guaranteed, the preparation process is simple, special equipment is not needed, the cost is low, and the paint is very suitable for industrial production and is particularly suitable for protection in high-temperature and high-humidity environments such as basements, brewing workshops and coastal buildings.

The invention provides an engineered cell for enhancing tumor killing ability and application thereof. The engineered cell is integrated with polynucleotide for coding and expressing secretory protein at a fixed point; the secretory protein comprises (1) a first secretory protein and a second secretory protein; the first secretory protein is an inhibitor of an immune checkpoint molecule PD1; the second secretory protein is different from the first secretory protein; or (2) a double antibody, polyclonal antibody or fusion protein comprising a domain having a specific binding immune checkpoint molecule PD1; the engineered cell is prepared in a non-virus manner. According to the scheme adopted by the invention, tumor cells are efficiently killed at a tumor part, the safety is higher, and the tumor killing capability is stronger.

The application provides an antibody or fragment thereof of an activation receptor (OX40) expressed on the surface of activated CD4+ T and CD8+ T cells, and use of the antibody or fragment thereof for preventing or treating diseases. The antibody of the application has high affinity for OX40, obvious effect of activating the OX40 signal pathway, relatively broad immune enhancement effect, and the ability of enhancing T cell response immune memory. In an animal model in vivo experiment, good tumor inhibition and killing effects are achieved, and the antibody has good clinical application prospect.