74 results about "Streptococcus halichoeri" patented technology

The invention discloses a high-affinity monoclonal antibody for recognizing group B streptococcus SIP (Session Initiation Protocol) protein or an antigen binding fragment thereof. The amino acid sequence of a heavy chain variable region of the antibody is as shown in SEQ ID NO.1, and the amino acid sequence of a light chain variable region of the antibody is as shown in SEQ ID NO.5. The lowest detection limit of the group B streptococcus SIP protein double-antibody sandwich enzyme-linked immunosorbent assay established by taking the monoclonal antibody or the antigen binding fragment thereof as a capture antibody on the SIP protein is 0.32 ng / mL. The lowest detection limit of a group B streptococcus SIP protein double-antibody sandwich fluorescence immunochromatography assay method established by taking the monoclonal antibody or the antigen binding fragment thereof as a capture antibody on SIP protein is 0.08 ng / mL, and the lowest detection limit on GBS bacterial liquid reaches 1 * 10 < 3 > CFU / mL, which is obviously higher than the lowest detection limit of the existing detection reagent and literature report; the method can be used for qualitative and quantitative detection of the group B streptococcus SIP protein.

The invention discloses an NKT cell in-vitro amplification method based on a combined stimulant, and belongs to the technical field of biology. The method comprises the following steps: firstly separating mononuclear cells in autologous peripheral blood, and then performing induced activation and amplification on the NKT cells in a serum-free culture system by adding a group A streptococcus preparation, a zoledronic acid preparation and a cytokine IL-2 as a combined stimulant. The method is easy to operate, efficient amplification of the NKT cells can be achieved only through three key reagents, the obtained NKT cells have the advantages of being large in number, high in purity, high in cytotoxicity and the like, and the requirements of clinical treatment can be met. Compared with a traditional method, the culture cost is remarkably reduced, the operation process is simplified, large-scale production, popularization and application are easy, and an efficient and economical technical scheme is provided for NKT cellular immunotherapy.

The invention relates to the technical field of biological analysis and detection, and particularly discloses a streptococcus mutans detection kit based on an anchored CRISPR-Cas12a enhanced paper-based micro-fluidic chip and a detection method. The detection kit comprises an RAA solution or RAA freeze-dried powder, magnesium acetate or a magnesium acetate solution, TMB or a TMB solution, an RAA forward primer, an RAA reverse primer, a cell lysis buffer, a buffer system and a paper-based micro-fluidic chip. The detection method comprises the following steps: establishing a standard curve and detecting a sample. The detection kit is used for detecting the content of streptococcus mutans and has the characteristics of high efficiency, rapidness, specificity, sensitivity, portability and the like, and the method is simple and convenient to operate, low in detection cost and capable of being applied to on-site detection of streptococcus mutans.

The application discloses a kind of fluorescence colorimetric double-mode detection streptococcus agalactiae without aerosol pollution method, comprising the following steps: (1) strain culture and DNA extraction;(2) the combination of UDG and LAMP;(3) UDG-LAMP-CRISPR fluorescence mode detection system;(4) colorimetric mode detection system: read image RGB value in G value, G value significantly increases, and it is positive result.This patent innovatively combines UDG, LAMP, CRISPR and G4, introduces connection probe to realize "fluorescence-colorimetric" single system double-mode detection.The two detection modes of the detection platform can be freely split or combined, and can meet the needs of different detection environments.By combining UDG system and LAMP technology, aerosol pollution possibly carried in amplification process is avoided.Connection probe is introduced, CRISPR fluorescence detection is combined with G4 colorimetric detection, a detection system with "fluorescence-colorimetric" two detection modes is established, which is used for quantitative identification and cross verification of streptococcus agalactiae.

The invention discloses a group B streptococcus detection system and method, relates to the technical field of biology, and aims to solve the problem that the existing group B streptococcus detection method cannot give consideration to detection efficiency, sensitivity and specificity at the same time. The detection system comprises a PCR amplification product aiming at a cfb gene specific fragment, crRNA, LbaCas12a and a single-chain DNA fluorescent reporter group, the PCR amplification of the specific fragment of the cfb gene comprises a forward / reverse primer GBS-F / R; wherein the sequence of the crRNA is as shown in one of SEQ ID NO: 1 to SEQ ID NO: 4. When a PCR amplification product of a reaction substrate cfb gene is in a low-concentration condition (10pg), high-sensitivity detection can be realized, and the method has the advantage of low-abundance sample detection.

The technology proposed herein concerns a vaccine formulation comprising a) an antigen comprising an amino acid sequence having at least 90% sequence identity with the amino acid sequence of the N-terminal region of a first group B Streptococcus surface protein, and b) a buffer having a pH of 6 to 8 and comprising: a. aluminum hydroxide gel particles, and b. a buffer system comprising: i. 1-3 mM phosphate and 100-200 mM NaCl, or ii. 5-15 mM histidine and 200-400 mM sorbitol. The technology proposed herein further concerns a method of producing a vaccine formulation as well as a vaccine formulation for use in a method of reducing, preventing and / or treating a GBS infection.

The invention provides a 19F streptococcus pneumoniae invasiveness risk prediction method, and relates to the technical field of molecular biology. Wherein the evaluation marker combination comprises a translation genome, a cell synthesis genome and a ligand transport metabolism genome; the translation genome comprises rluB and rsmE; the cell synthetic genome comprises mscL and murI; and the ligand transport metabolism genome comprises czcD, site B, mtsC and pstS. The evaluation marker combination can accurately evaluate the invasive risk of serotype 19F streptococcus pneumoniae, and realizes molecular typing and risk prediction. The kit has high sensitivity and specificity, is suitable for high-throughput detection, and is helpful for clinical diagnosis and pathogenic mechanism research.

The invention discloses a primer probe composition and a kit for group A streptococcus species identification and M1UK and M1global subtype typing and application of the primer probe composition and the kit. According to the primer probe composition, quadruple target amplification primers and probes are designed, so that specific amplification can be achieved in a single tube, and cross interference is avoided; the primer probe group forms a species-type-double SNP subtype three-stage detection system and is used for preparing detection kits for GAS species identification, em1 type confirmation, M1UK / M1global subtype typing and the like, interference of other em1 derived sublines is eliminated according to the double SNP co-occurrence requirement, and the M1UK typing accuracy is remarkably improved. The kit has the advantages of high specificity, high throughput, simplicity and convenience in operation and low cost, and is suitable for clinical diagnosis, high-virulence clone screening and public health emergency monitoring.

The present application relates to a Pichia pastoris indirect surface display system based on SpyC / T, construction and application thereof, and relates to the technical field of bioengineering. The Pichia pastoris indirect surface display system uses SED1 protein from Saccharomyces cerevisiae as an anchor protein, and fixes SpyC from Streptococcus pyogenes on the anchor protein on the surface of Pichia pastoris cells. The present application uses SED1 protein from Saccharomyces cerevisiae as an anchor protein, displays SpyC on the surface of Pichia pastoris, fixes various target proteins containing SpyTag on the cell surface through strong interaction of SpyC / T, so as to indirectly display the target proteins containing SpyTag on the surface of Pichia pastoris, exert the function of the target proteins containing SpyTag, and improve the stability of the target proteins.

The invention discloses a covalent organic framework for efficiently preventing and treating caries as well as a preparation method and application of the covalent organic framework. The covalent organic framework nano material prepared by the invention can be efficiently combined with the enamel surface, reduce the roughness of the enamel surface and efficiently inhibit the adhesion of streptococcus mutans, and can generate high-concentration active oxygen and strongly sterilize in combination with a photodynamic therapy, thereby efficiently preventing and treating the occurrence of decayed teeth.

A method for processing a saliva sample for downstream DNA analysis. The method includes mixing the saliva sample with a chelating resin solution to form a mixture, incubating the mixture at a temperature of 120° C. or lower and duration of at least 1 minute, wherein the temperature and duration are inversely related, shaking the mixture before and after incubation for a period ranging from 0 seconds to 2 hours, separating the mixture into a supernatant and a sedimented chelating resin using either centrifugation or by allowing natural gravitational settling, and recovering the supernatant for subsequent analysis.

This invention provides an L-lysine-producing strain, its construction method, and its application. The strain utilizes homologous recombination gene editing technology to first overexpress... dapA , aspB Introducing mutations ppc (A482V) Knockout amn Multiple copies are synchronized by manually manipulating Pa12-dapB(T1)-Psod-aspB(T1)-Pa16-Ec_lysA. dapB , aspB Ec. from E. coli W3110 lysA Multiple copies are synchronized via the artificial manipulator Pa12-ddh(RBS)-lysE(T1)-Pa16-gapN(RBS)-amtB. ddh , lysE Streptococcus equi subsp. porphyria gapN , amtB The strain is plasmid-free, defect-free, requires no induction, and has advantages such as good genetic stability and high fermentation yield, enabling stable production of L-lysine.

The invention discloses a group A streptococcus mRNA (messenger Ribonucleic Acid) vaccine and application thereof, and relates to the technical field of biological medicines, in particular to the group A streptococcus mRNA vaccine and application thereof. The mRNA for coding the group A streptococcus antigen contains an open reading frame; the nucleotide sequence of the coding open reading frame is M12 <-N > + M1 <-N > + M4 <-N >, and is as shown in SEQ ID NO. 1; the antigen sequence of the group A streptococcus mRNA vaccine has no homology with human tissue protein through blastp comparison; the mRNA vaccine has strong immunogenicity, can effectively inhibit three GAS serotypes, and covers main GAS epidemic strains in China at present; the mRNA vaccine has the advantages of high efficiency, broad spectrum, high safety and simplicity and convenience in use, and is easy to popularize and use.

The invention relates to the technical field of biological detection, in particular to a group B streptococcus rapid detection and serotyping kit based on a real-time fluorescent quantitative PCR (Polymerase Chain Reaction) technology. The invention provides a primer probe group with sequences as shown in SEQ ID NO: 1-18 and a kit, the group B streptococcus can be rapidly and accurately detected, 10 common serotypes (Ia, Ib, II, III, IV, V, VI, VII, VIII and IX) can be covered, and meanwhile, the common serotypes (Ia, Ib, II, III and V) in China are subjected to serotyping; the detection sensitivity of the optimized primer probe sequence can reach 50 copies / mL; the stability of the reagent at room temperature reaches 24 months, and the reagent is suitable for rapid screening of perinatal GBS infection and epidemiological typing research.

To provide a novel technique for removal of Fusobacterium and / or Streptococcus bacteria.SOLUTION: The foregoing problem is solved by a composition for removal of Fusobacterium and / or Streptococcus bacteria, containing platinum nanoparticles and / or electrolyzed water as an active ingredient.SELECTED DRAWING: None

Streptococcus pyogenes Cas9 (SpCas9) variants with relaxed PAM requirements capable of high-resolution editing for various applications, and methods of use thereof.

An analysis method includes: acquiring data corresponding to a mass spectrum obtained by subjecting a sample containing a microorganism to mass spectrometry; and acquiring information on Group A Streptococcus of emm type 1, based on the presence or absence, or magnitude of a peak in a first range of m / z of 10930 or more to 10945 or less in the mass spectrum.

The invention discloses construction and application of a streptococcus suis serotype 2 cps2D gene knockout mutant strain. The cps2D gene knockout mutant strain delta cps2D of the streptococcus suis serotype 2, and a coding gene between the 31st site and the 1000th site of the cps2D gene in the 05ZYH33 strain is replaced by a spectinomycin resistance gene cassette. Capsules of the mutant strain are obviously reduced, the capability of resisting phagocytosis of macrophages is reduced, and animal toxicity test results show that the toxicity of the mutant strain is obviously reduced. The mutant strain provides an important clue for screening of protective antigens of multivalent subunit vaccines, and can be applied to development of S.suis attenuated vaccines and multivalent subunit vaccines.

The invention relates to the technical field of microbiological detection, in particular to a Brucella bovis and Mycobacterium bovis dual-fluorescent microsphere test strip and a preparation method and application thereof, and the Brucella bovis and Mycobacterium bovis dual-fluorescent microsphere test strip comprises a bottom plate, a sample pad, a nitrocellulose membrane coated with recombinant protein and absorbent paper, the nitrocellulose membrane coated with the recombinant protein is provided with a brucella abortus lipopolysaccharide detection line T1, a mycobacterium bovis rMPB70 protein detection line T2 and a recombinant streptococcus G (rSPG) protein control line C, and the brucella abortus lipopolysaccharide detection line T1, the mycobacterium bovis rMPB70 protein detection line T2 and the rSPG protein control line C are arranged in parallel. The detection time is short, the stability is good, the operation is simple, the sensitivity is high, complex instruments and professional personnel are not needed, and the result judgment is visual and reliable.

The present invention discloses a kind of group B streptococcus instant detection device based on isothermal amplification and CRSPR / Cas12a technology, it is characterised in that it includes microfluidic chip and supporting instant detection device, wherein: microfluidic chip includes two detection liquid channels, two detection liquid channels eventually intersect in detection chamber, and two detection liquid channels are respectively supplied to isothermal amplification system and CRSPR / Cas12a system detection liquid droplet; Instant detection device includes upper cover, housing, chip slot, amber filter plate, LED light source, light path channel, observation window, PTC heating plate, battery box and switch. The present invention can complete nucleic acid amplification and fluorescence detection within 40 minutes, and the closed microfluidic chip reaction system can effectively reduce aerosol generation; The present invention is more convenient for obstetrics and individuals to carry out instant rapid detection of group B streptococcus compared to large molecular detection device.

The present invention relates to molecular biology and aquaculture breeding, specifically to a method for obtaining a PRMT2 gene mutant by knocking out the gene based on CRISPR / Cas9 technology, and establishing an antibacterial strain of tilapia with a PRMT2 knockout. By knocking out this gene, a tilapia strain resistant to Streptococcus agalactiae can be obtained. Infection experiments revealed that tilapia with a PRMT2 knockout gene exhibited enhanced antibacterial activity compared to wild-type tilapia. This invention provides an important model for disease-resistant tilapia breeding and offers gene targets and new research directions for creating more disease-resistant fish strains and varieties.

The application relates to the technical field of biological detection, in particular to a Streptococcus thermophilus oligonucleotide probe and a preparation method and application thereof; the oligonucleotide probe has a nucleotide sequence as shown in SEQ ID NO. 1. The V1 region of the 16S rRNA gene has conservation and specificity, the V1 region of the 16s rRNA sequence of the Streptococcus thermophilus is used as a target gene, a plurality of oligonucleotide probes with a length of 20 bp are designed, the non-specificity of the oligonucleotide probe can be effectively reduced, then the oligonucleotide probes are searched and analyzed in a nucleotide sequence library, oligonucleotide probes meeting the requirements of fluorescence in situ hybridization can be screened out, finally, the oligonucleotide probes are actually detected by using a plurality of strains, and the oligonucleotide probes with higher specificity can be screened out and combined with the Streptococcus thermophilus of a sample to be detected, so that the accuracy of the oligonucleotide probe in fluorescence in situ hybridization and flow analysis technology can be improved.

The invention provides a group B streptococcus antigen detection kit, which belongs to the technical field of antigen detection, and comprises the following components: a reagent card, an ID card, an extraction liquid and an extraction tube, the reagent card comprises a reagent strip, a light-transmitting film and a card shell; the reagent strip comprises a sample pad, a combination pad, a nitrocellulose membrane, an absorption pad and a PVC (Polyvinyl Chloride) bottom plate; the combination pad contains a group B streptococcus antibody a marked by latex microspheres and chicken IgY marked by fluorescent microspheres; the nitrocellulose membrane contains a group B streptococcus antibody b and a goat anti-chicken IgY polyclonal antibody. According to the technical scheme provided by the invention, the antibody with high sensitivity and strong specificity is prepared, and meanwhile, the false positive interpretation is reduced.

This invention provides a non-classical crRNA, reagent, kit, and application for one-step RPA-Cas12a nucleic acid detection. Based on classic crRNA, the non-classical crRNA shortens the 5' end by two bases and / or adjusts the stem-loop base structure to one of UCUUUU, UCUUU, UGUUU, UGUU, or UAAGU. This non-classical crRNA structure reduces the affinity of the Cas12a-crRNA complex for the target DNA, weakens the cis-cleavage activity of Cas12a, and increases the trans-cleavage activity of Cas12a, without requiring additional base modifications. This invention achieves a detection limit of 1 fM in the detection of Group B Streptococcus and Human Papillomavirus 16, with a sensitivity approximately 10-fold higher than before optimization.

The invention provides a simple and efficient screening method of group A streptococcus qPCR primers, and belongs to the technical field of qPCR primer screening, and the method comprises the following steps: taking group A streptococcus as a research object, on the basis of satisfying basic specificity, designing key factors which significantly influence the performance of the qPCR primers; opinions of employees in the field of qPCR detection in the industry are collected, and importance coefficients of all factors influencing the performance of the group A streptococcus qPCR primer are obtained based on a DARE method; on the basis of Primer Premier 5.0 software and on the basis of ensuring specificity, qPCR primers are designed, and each group of primer pair comprises an upstream primer and a downstream primer; a statistical quartile method is used for quartering factor levels influencing the primer performance in a reasonable interval, the factor levels are Q1-Q4 respectively, assignment is carried out respectively, and summing is carried out in a group; and screening the first several groups of primers with the highest assignment, and detecting the actual specificity and amplification efficiency of the primers through a probe method to screen the optimal primer. According to the technical scheme provided by the invention, the qPCR primer of the group A streptococcus can be simply, conveniently and efficiently screened.

The invention discloses a sheep colostrum composition capable of improving sleep and application of the sheep colostrum composition, and belongs to the technical field of microbial fermentation and functional food. The core of the invention lies in that a composite leavening agent is compounded by using the casei paracasei GP66 and the streptococcus salivarius subsp. Thermophilus HX-ST36, and then the sheep colostrum of concentrated whey protein, galactooligosaccharide and acerola cherry powder is subjected to synergistic fermentation. According to the fermentation process, IgG in sheep colostrum can be efficiently reserved, and the content of small molecule peptide and gamma-aminobutyric acid in a fermentation system is remarkably increased. Meanwhile, the invention also generates a novel pentapeptide, the pentapeptide has a sleep aiding effect, and the amino acid sequence of the pentapeptide is Arg-Phe-Leu-Trp-Pro (RFLWP). Animal experiments prove that the traditional Chinese medicine composition can play a role in improving sleep by regulating a neurotransmitter system. The invention provides a new way of a product for improving sleep, which is natural, safe and clear in mechanism.

The invention relates to the technical field of molecular biology detection, in particular to a cattle and sheep universal type echinococcosis ELISA diagnostic kit and application thereof.The cattle and sheep universal type echinococcosis ELISA diagnostic kit comprises an SPG-HRP conjugate, the SPG-HRP conjugate is obtained by coupling SPG recombinant protein and horse radish peroxidase, the SPG recombinant protein comprises r2CSPG and r3CSPG, the amino acid sequence of the r2CSPG recombinant protein is shown as SEQ ID NO.1, the amino acid sequence of the r3CSPG recombinant protein is shown as SEQ ID NO.2, and the amino acid sequence of the r3CSPG recombinant protein is shown as SEQ ID NO.2. The amino acid sequence of the r3CSPG recombinant protein is as shown in SEQ ID NO. 2. The problem of how to apply the streptococcus protein G to early screening and early diagnosis of echinococcosis is solved.