98 results about "Streptococcus halichoeri" patented technology

The invention belongs to the field of genetic engineering, discloses a streptococcus thermophilus derived target sequence recognizable by a CRISPR (clustered regularly interspaced short palindromic repeat)-Cas9 (CRISPR associated 9) system and further discloses a sgRNA (single guide ribonucleic acid) and a coding DNA (deoxyribonucleic acid) molecule thereof. The target sequence is shown as n-20th of any one of SEQ ID NO:1-63, wherein n=1-5. A sequence of the sgRNA is 5'-recognition sequence- Cas9 protein recruitment sequence-3', and a DNA sequence corresponding to the recognition sequence is identical to the target sequence. The invention also discloses the CRISPR-Cas9 system, and the CRISPR-Cas9 system comprises Cas9 proteins and sgRNA and/or comprises carriers carrying a Cas9 protein coding sequence and a sgRNA coding sequence. The invention further discloses application of the CRISPR-Cas9 system to CXCR4 gene editing and preparation of medicines for treating HIV (human immunodeficiency virus) infection. CXCR4 gene editing can be realized to protect cells from HIV infection.

The invention discloses a Streptococcus faecalis powdering LAMP rapid detection kit, and a rapid detection method based on the kit. The kit comprises a freeze-dried isothermal amplification agent, a re-dissolving solution, a positive contrast dry powder, a negative contrast substance, a colorimetric solution, a lysate and a sealing solution, and the freeze-dried isothermal amplification agent contains dNTPs, Bst DNA polymerase, an amplimer and a freeze-drying protection agent. The primer in the kit corresponds to eight segments of a Streptococcus faecalis specific gene sequence, the colorimetric solution is added after a reaction, and a negative and positive colorimetric difference is obvious. The kit has the advantages of strong specificity, high sensitivity, easiness in operation and simplicity in result judgment, and is suitable for the quality control and the safe and rapid detection of foods, beverages, water and other raw and auxiliary materials. The kit is convenient for normal temperature transportation, is simple and fast to use without special devices, reduces the cost, widens the application range of products, is suitable for onsite rapid detection and base remote region related detection, and has wide application prospect.

To address the limitations deriving from the unspecific genomic cleavages of the Streptococcus pyogenes Cas9 (SpCas9) and to identify variants with higher cleavage fidelity, the present invention describes a yeast-based assay which allows to simultaneously evaluate the on- and off-target activity towards two engineered genomic targets. The screening of SpCas9 variants obtained by random mutagenesis of the Red-II domain allowed the identification of hits with increased on/off ratios. The best performing nuclease, evoCas9, was isolated through the combination of the identified mutations within a single variant. Side by side analyses with previously reported rationally designed variants demonstrated a significant improvement in fidelity of evoCas9 of the present invention.

The present invention relates to polyresistin dripping pill and its preparation process. Type-A streptococcus hemolyticus strain is deeply cultured and extracted to obtain one kind of refined polysaccharide substance polyresistin with immunoactivity and antitumor activity and named alpha-mannose polypeptide chemically; polyresistin is added into molten matrix via stirring to form pill; and the pill is cooled in cooler and dried to prepare the polyresistin dripping pill. Preparing into dripping pill can raise the stability and biological utilization of the medicine for quick and lasting effect.

The present invention provides capsular polysaccharides from Streptococcus pneumoniae serotypes identified using NMR. The present invention further provides polysaccharide-protein conjugates in which capsular polysaccharides from one or more of these serotypes are conjugated to a carrier protein such as CRM197. Polysaccharide-protein conjugates from one or more of these serotypes may be included in multivalent pneumococcal conjugate vaccines having polysaccharides from multiple additional Steptococcus pneumoniae serotypes.

The invention relates to a novel bacillus subtilis multi-valent vector-based vaccine and application thereof. The novel bacillus subtilis multi-valent vector-based vaccine comprises a bacillus subtilis recombination strain obtained by using a genetic engineering method, wherein the bacillus subtilis recombination strain is classified and named as bacillus subtilis HT5302 and is preserved in the China Center For Type Culture Collection (CCTCC) with a preservation number of CCTCC No:M2012380. The novel bacillus subtilis multi-valent vector-based vaccine is prepared by using the bacillus subtilis recombination strain as a core. A spore with a surface having no a streptococcus agalactiae 3-glyceraldehyde-phosphate dehydrogenase (GAPDH) protein can be generated through inducing the bacillus subtilis recombination strain, and secretes a fusion protein which formed by cell-penetrating peptide and vibrio anguillarum 3-GAPDH when germinating to form a nutrient cell. The novel bacillus subtilis multi-valent vector-based vaccine has a multi-valent protection function on bacterial diseases such as streptococcicosis, vibriosis, edwardsienosis and aeromonas hydrophila.

The invention relates to a set of base editing artificial system for paddy. The system comprises an I adjusting element and an II adjusting element. The I adjusting element can encode a nucleotide sequence of an amino acid sequence I, wherein the amino acid sequence I comprises an amino acid sequence shown in SEQ ID No.1. The II adjusting element comprises an (II-1) nucleotidesequence and an (II-2) nucleotide sequence from the end 5' to the end 3' in sequence. The (II-1) nucleotide sequence comprises a target nucleotide sequence, the (II-2) nucleotide sequencecomprises an sgRNA nucleotide sequence derived from streptococcus pyogenes, and the (II-1) nucleotide sequence and the (II-2) nucleotide sequence are in transcription fusion.

The invention provides a LAMP (Loop-Mediated Isothermal Amplification) primer group, a micro-fluidic chip and a kit for detecting reproductive tract pathogenic microorganisms, and belongs to the technical field of reproductive tract infection detection. The LAMP primer group comprises a streptococcus agalactiae detection primer group, an enterococcus faecalis primer group, a gardnerella vaginosisprimer group, a Candida albicans primer group and a Chlamydia trachomatis primer group. The above LAMP primer group is fixed on the micro-fluidic chip. The LAMP primer group, the micro-fluidic chip and the kit can quickly, sensitively and accurately detect streptococcus agalactiae, enterococcus faecalis, gardnerella vaginosis, Candida albicans and Chlamydia trachomatis by high repeatability.

The invention discloses a group A streptococcus antigen detection test strip and kit and preparation methods thereof, and relates to the technical field of group A streptococcus detection. The group Astreptococcus antigen test strip is prepared by sequentially attaching a nitrocellulose membrane coated with an anti-group A streptococcus detection antibody, an immune binding pad coated with an anti-group A streptococcus immunolabeled antibody, absorbent paper and a sample pad to a polyvinyl chloride bottom plate; a detection card can detect whether a group A streptococcus antigen is present ina sample to be detected or not by detecting a marker; and the test strip and the detection card comprising the test strip can specifically and quickly detect and diagnose early infection of group A streptococcus, thereby reducing the cost, realizing rapid detection and meeting the requirements of clinical use.

The invention relates to a primer and probe group and kit for detecting hemolytic streptococcus through an RAA fluorescence method, and belongs to the technical field of molecular biological detection. The primer and probe group for detecting the hemolytic streptococcus through the RAA fluorescent method comprises an upstream primer, a downstream primer and a probe; the primer and probe group is characterized in that the nucleotide sequence of the upstream primer is shown as SEQ ID NO.1; the nucleotide sequence of the downstream primer is shown as SEQ ID NO.2; and the probe is a substance which includes the 40th-site base modified fluorescence reporter gene, the 42nd-site base modified tetrahydrofuran residue and the 43rd-site base modified quenching group, and the nucleotide sequence of the probe is shown as SEQ ID NO.3. The kit provided by the invention is rapid and sensitive in detection and simple and convenient to operate; the kit is of great significance to spread of the hemolytic streptococcus in the future; and the kit has a relatively high application prospect.

The invention relates to an excellent microorganism strain for space mutation, in particular to a good microorganism strain for space mutation, and the preparation method and the application thereof. The discoveries that the bump of a cancer patient infected by hemolytic streptococcus can subsidise naturally and the hemolytic streptococcus and the preparation thereof have a certain of biological activities are made is as early as in the 19th century. In the invention, the special environment of the outer space is utilized to deliver the strains to the outer space through carrying the 22nd recoverable experimental science satellite, the 22nd recoverable experimental science satellite safely returns after traveling for a certain period of time, the suppresormutation strains are eliminated, the forward mutation strains with excellent features are selected out for re-screening, and ultimately the excellent microorganism strains with vigorous life, quick growth speed, high fermentation yield, short fermentation period, high polypeptide content, stable hereditary property, strong productive adaptability and space cumulative effect. The microorganism strain can be applied to the strains for industrialization production, and has the advantages of high output, wide application range, low cost and high effective component content.

The invention belongs to the technical field of oral preventive medicine, and particularly provides an anti-caries DNA (Deoxyribose Nucleic Acid) vaccine. The anti-caries DNA vaccine is prepared by extracting the protein full-length gene of a wall-connected associated protein A (wapA) from a streptococcus mutans UA159 genome and cloning into a eukaryotic expression vector pVAX1. In order to enhance the immune capacity of the anti-caries DNA vaccine, the anti-caries DNA vaccine is prepared into a chitosan nanometer compound by taking chitosan as a vector. The invention also provides a preparation method and application of the anti-caries DNA vaccine. The anti-caries DNA vaccine provided by the invention has the advantages of high safety, natural antigen expression, lasting immune response and effective caries prevention.

The present invention provides a number of process improvements related to the conjugation of capsular polysaccharides from Streptococcus pneumoniae to a carrier protein. These process are serotype specific and include acid hydrolysis, addition of sodium chloride to the reductive amination reaction, and addition of sucrose to dissolve polysaccharides. Polysaccharide-protein conjugates prepared using the processes of the invention can be included in multivalent pneumococcal conjugate vaccines.

The invention discloses extraction equipment with a self-cleaning mechanism for streptococcus vaccine preparation, relates to the technical field of devices for vaccine preparation, and aims to solve the problem that most of extraction equipment in the market at present performs rigid clamping through mechanical arms and cannot perform clamping adjustment according to the shape and position of a packaging device. A cleaning pool is arranged on one side of the base, a third driving arm motor is arranged in a third driving arm, a first rotating disc is arranged below the third driving arm motor in a transmission mode, a first telescopic rod is fixedly arranged below the first rotating disc, and a fixing plate is fixedly arranged below the first telescopic rod. A grabbing mechanism or an ampoule clamping mechanism is fixedly installed below the fixing plate through a buckle, a chuck is arranged at the upper end of the grabbing mechanism, a grabbing air bag is arranged at the lower end of the grabbing mechanism, a clamping fixing base is arranged at the upper end of the ampoule clamping mechanism, and a plurality of ampoule limiting bases are arranged below the clamping fixing base in a rectangular mode.