7results about How to "Highly specific detection" patented technology

The invention provides a blood coagulation factor activity detection system and method and a storage medium, and the system comprises a quantum dot fluorescent probe array module which comprises a plurality of quantum dot fluorescent probes and is used for identifying and marking different blood coagulation factors in a blood sample; the laser module is used for emitting laser beams with various preset wavelengths to the blood sample, and the various preset wavelengths are matched with excitation wavelengths of different quantum dots; the signal capturing module is used for continuously capturing a fluorescence signal corresponding to each quantum dot fluorescence probe and transmitting the fluorescence signal to the activity detection module in real time; and the activity detection module is used for recording fluorescence change signals corresponding to different blood coagulation factors, labeling blood coagulation factor types for the fluorescence change signals, and inputting an activity prediction model to obtain activity levels of different blood coagulation factors. The multi-quantum-dot fluorescent probe can efficiently detect the activity of various blood coagulation factors, and the comprehensiveness and accuracy of blood coagulation factor activity detection are improved.

The invention discloses a kit for detecting RNA (Ribonucleic Acid) of a hepatitis E virus based on RAA (Reverse Amplification Amplification) / PCR (Polymerase Chain Reaction)-CRISPR-Cas13a. The kit comprises a primer pair used for amplifying or detecting an HEV conserved gene segment (SEQ ID No.1) and crRN A, and the sequence of crRNA is composed of an anchoring sequence and a guide sequence (39-66th sites of SEQ ID No.13). Based on a CRISPR-Cas13a nucleic acid detection technology, through design, construction and screening, a set of RAA amplification primer pair or PCR primer pair for hepatitis E virus RNA detection, crRNA and a detection method thereof are finally provided, the crRNA can realize high-sensitivity and high-specificity detection of hepatitis E virus RNA by activating Cas13a, and the sensitivity reaches 1 copy / mu L. The method is simple, convenient and rapid, and has important application value.

The invention belongs to the technical field of immunodetection, and particularly relates to an unlabeled multiple immunodetection system based on core-shell photon bar code integrated rolling circle amplification and application. The non-labeled multiple immunodetection system comprises: 1) a probe-modified porous hydrogel-PhC shell microsphere, the probe is used for capturing a target nucleic acid molecule to be detected, the porous hydrogel-PhC shell microsphere is formed by coating a silica microsphere with a porous hydrogel, and the diameter of the porous hydrogel-PhC shell microsphere is 200-200 [mu] m; 2) an antibody modified by a DNA promoter; 3) a blocking / primer compound; and 4) a circular DNA. The detection system provided by the invention realizes high-sensitivity multiple quantitative detection, and the detection limit is within the range of 0.030 pg / mL. The detection system provided by the invention is verified through clinical sample analysis, has acceptable accuracy and high specificity, and proves that the provided detection system and method have good potential applicability in clinical screening and diagnosis.

The invention provides a molecular imprinting fluorescence sensor for insulin autoantibody detection and a preparation method, and relates to the technical field of biological medicines.The molecular imprinting fluorescence sensor is characterized in that an aptamer capable of specifically recognizing IAA antibodies related to type 1 diabetes is used as a template, a template coordination fixing method is utilized, dopamine is used as a functional monomer, and the molecular imprinting fluorescence sensor is prepared through an auto-polymerization method. Fluorescent responsive imprinted nanoparticles based on fluorescent quantum dots are prepared, and PEG is grafted on the surface of the prepared fluorescent imprinted material by using a simple post-modification pegylation method. The molecular imprinting fluorescence sensor is used for quantitative detection of IAA < + > in a complex blood environment, especially under the individual condition of single autoantibody positive, not only can reduce the false positive rate and improve the disease predictability, but also provides a more accurate basis for risk prediction screening, typing, curative effect evaluation and guided treatment of early diabetes mellitus, and has good application prospects. And important support is provided for personalized diagnosis and treatment of diseases.

The invention discloses a double-frequency single-walled carbon nanotube terahertz metasurface biosensor with high sensing performance, which is composed of two parts, the biosensor comprises an upper carbon nanotube resonator layer and a lower substrate, the resonator layer is composed of double-ring unit structures arranged in a periodic array, all the double-ring unit structures are the same, and the upper substrate and the lower substrate are arranged in parallel. Each unit structure is composed of two circular rings with the middle portions overlapped with each other, and the middles of the two circular rings are communicated. The terahertz metasurface sensor with high sensitivity is realized by combining the excellent photoelectric characteristics of the carbon nanotubes.

This invention discloses an anti-Dabie Banda virus Gn protein nanobody, a bispecific antibody, and their applications, belonging to the field of biodetection technology. This invention constructs an alpaca natural nanobody library, using recombinantly expressed Gn protein as the antigen. Through multiple rounds of gradient screening using phage display technology, three nanobodies—G15, G20, and G40—that specifically bind to the Dabie Banda virus Gn protein are obtained. All three antibodies exhibit excellent antigen-binding specificity and virus-neutralizing activity; among them, G40 shows the most prominent neutralizing effect. The bispecific antibody G40-C1q, constructed based on G40, combines Gn protein and human C1q protein, achieving a synergistic effect of antiviral activity and inhibition of inflammatory damage. It can be used for the development of drugs for the prevention and treatment of Dabie Banda virus infection and for high-specificity detection reagents, providing candidate antibody drugs for the treatment of fever with thrombocytopenia syndrome, and providing a reliable tool for virus-related research, disease diagnosis, and clinical translation.

The present invention discloses a cascade signal amplification detection method based on platinum nanozymes, comprising the following steps: (1) constructing a magnetic nanoprobe; (2) constructing a platinum nanoprobe; (3) constructing a sandwich immune sandwich structure; (4) inducing a cascade signal amplification reaction; (5) visual quantitative detection of analytes. On the one hand, the present invention utilizes the specific recognition principle of antibodies to antigens to construct a sandwich immune sandwich structure, and on the other hand, utilizes platinum nanozymes to induce a cascade signal amplification reaction, thereby realizing the conversion of analyte recognition signals to visual ink advance distances, and at the same time realizing ultrasensitive quantitative detection of analytes, and applying this to the detection of ovarian cancer biomarkers. The detection method is low in cost, simple in operation, short in reaction time, high in sensitivity and strong in specificity, providing a new analytical means for the instant diagnosis of cancer biomarkers, and can be extended to the detection of more biomarkers.