72results about How to "Achieve signal amplification" patented technology

The invention discloses a biomacromolecule detection platform of a magnetic particle microarray, which is established by combining magnetic nanoparticles and biochips with microfluidics technology. The magnetic nanoparticles are taken as a biomacromolecule carrier in the magnetic particle microarray. A DNA probe or an antibody is fixed on the surface of the magnetic particles to allow the magnetic particles to cross or react with a sample to be detected, thus various samples to be detected are fixed on the surface of the magnetic particles, and then the magnetic particles are spotted in a microfluid reaction cell with a magnetic substrate to form the magnetic particle microarray. A fluorescent probe or a fluorescent labeled antibody is added to the microfluid reaction cell and reacts in the reaction cell, and an obtained mixture is washed, thus obtaining information of various samples by a fluorescent biochip scanner.

The invention relates to a dual-label rapid response nucleic acid adapter probe, which consists of a nucleic acid adaptor, the 5' end and 3' end of which are labeled by a fluorescent chromogenic grouprespectively, and a complementary probe, the 5' end and 3' end of which are labeled by a fluorescent quenching group respectively. The nucleic acid adaptor is a nucleic acid adaptor which can especially identify aflatoxin B1, namely, AFB1 nucleic acid adaptor. The nucleotide sequence of the AFB1 nucleic acid adaptor is shown as SEQ ID NO: 1 in the sequence list. The complementary probe is complementary to two ends of the AFB1 nucleic acid adaptor. The nucleotide sequence of the complementary probe is shown as SEQ ID NO: 2 in the sequence list. The method can be used for rapidly detecting aflatoxin B1. The identification process, after the aflatoxin B1 is added, can be completed in one minute, and one aflatoxin B1 molecule can trigger the recovery of two fluorescent group signals with highsensitivity. The whole reaction is carried out in a homogeneous and constant temperature solution environment, and the operation is simple.

The invention relates to a continuous spectrum light source comprising a stabilized voltage power supply, a light source, an optical fiber light source set, an optical fiber coupling lens set, and an output optical fiber. The optical fiber light source set is composed of N light spectrum channels with different wavelength coverage. Each light spectrum channel comprises orderly arranged component of a collimating lens, a band pass filter, a coupling lens, and an optical fiber. Light energies from the light source pass the collimating lens, such that parallel lights are formed; when the parallel lights pass the band pass filter, light energies with wavelengths within a range of (lambda1<(j)>, lambda2<(j)>) enter the optical fiber through the coupling lens. Optical fibers of the N light spectrum channels form an optical fiber bundle. The light energies are coupled into the output optical fiber through the optical fiber coupling lens set. Through the adjustment of light energy distribution in each light spectrum channel of the optical fiber light source set, the balance of the light energies output by the output optical fiber within an entire spectral range can be realized, such that a continuous spectrum light source is composed. Or, according to a spectrum energy distribution requirement, optical coupling efficiency of each spectrum channel of the optical fiber light source set can be adjusted, such that light energies can be arbitrarily distributed according to wavelengths.

The invention discloses a preparation method of a photoelectrochemical aptamer sensor for detecting a prostate-specific antigen. Lots of cadmium sulfide quantum dots and a signal transduction probe having one end marked with graphene quantum dots are loaded on a paper-based platinum/zinc oxide nanosheet and a co-sensitization effect is activated, so that a photocurrent signal is increased; when anaptamer of a specific recognition prostate-specific antigen with gold nanoparticles labeled and the signal transduction probe are hybridized to form three strings of helical molecular switches, the co-sensitization effect is suppressed and a signal quenching effect of the gold nanoparticle is generated, and the photocurrent signal decreases; and when the prostate-specific antigen identifies the aptamer, the conformation of the three strings of helical molecular switches changes, the co-sensitization effect is reactivated, the quenching effect is eliminated, and the photocurrent signal is enhanced. And the sensitive detection of the prostate specific antigen is realized based on the converted signal.

The invention discloses an integrated ELISA (Enzyme-Linked Immuno Sorbent Assay) chip based on distance detection targets and a detection method thereof. The integrated ELISA chip is provided with a plurality of water-phase cavities and oil-phase cavities which are alternatively arranged into a line, and front cavity bodies are communicated with rear cavity bodies; water-phase inlets are formed in the water-phase cavities; oil-phase inlets are formed in the oil-phase cavities; the water-phase cavity in the leftmost side is connected with a dye cavity which is additionally provided with a third inlet; the dye cavity is communicated with a dye gas column channel; and scales are arranged beside the dye gas column channel. The integrated ELISA chip disclosed by the invention integrates fussy steps of immunological experiment, cleaning and distance signal output onto a microfluidic pneumatic chip, and can be used for high-sensitivity quantitative instantaneous detection of multiple targets such as protein and cells and the like.

The invention discloses a photo-thermal release signal enhanced type thyroglobulin electrochemiluminescence immunosensor. According to a construction method of the sensor, a silicon-based titanium dioxide magnetic bead is used as a substrate to fix a thyroglobulin antibody, mesoporous silica marked thyroglobulin adsorbing bipyridyl ruthenium is used as a signal probe, and a competitive immunosensor is constructed through specific recognition between an antigen and the antibody. An electrode for modifying the antibody is placed into a mixed solution containing different concentrations of free antigens and a certain amount of signal probes, so that the free target antigen and the signal probe compete to bind the antibody, and a certain luminous intensity can be generated by combining the electrode of the signal probe. Under the irradiation of an 808 nm infrared laser, a sensing interface is rapidly heated, the dipyridyl ruthenium adsorbed by the mesoporous silica is released, and a luminous signal is remarkably enhanced along with the temperature rise, so that signal amplification is realized. As the concentration of the free target is increased, the number of the signal probes combined with the antibody is reduced, the luminous intensity is also reduced, and an electrochemiluminescence analysis method for the thyroglobulin can be established on the basis of the phenomenon.

The invention provides a surface plasma resonance sensor for detecting miRNA-21. The surface plasma resonance sensor comprises a working chip, wherein the working chip is formed by fixing a capture probe aiming at miRNA-21 on a bare gold chip. The miRNA-21 is detected by using the sensor disclosed by the invention; a calibration curve is subjected to four-parameter fitting; a regression equation refers to Y=(A1-A2)/(1+(X/X0)P)+A2, wherein A1 is equal to -69.95+/-7.34, A2 is equal to 2890.50+/-18.50, X0 is equal to 5.27+/-0.16, and P is equal to 0.47+/-0.01; the detection range is between 0.01nM and 1000nM; the correlation coefficient is 0.9978; and the lowest detection limit is 0.009nM. Compared with the prior art, the non-enzyme and label-free sensor can realize real-time monitoring, is high in sensitivity, high in specificity, low in cost, easy and convenient to operate and short in detection period, is suitable for measuring actual samples and the like and is expected to become a sensor with actual application value.

The invention discloses a high-throughput single-cell genome 5-hydroxymethylpyrimidine single-molecule visual analysis method, belonging to the technical field of analytical chemistry. According to the invention, a microfluidic chip technology is utilized to obtain agarose gel microspheres wrapping a single-cell genome; and after single cells in the agarose gel microspheres are subjected to cracking treatment, each agarose gel microsphere wraps a single cell genome. Primers are marked on 5-hmC and 5-hmU of the single-cell genome through a specific chemical reaction; rolling circle amplification is then performed; a fluorescent probe is hybridized; and single-molecule visual analysis of modified bases 5-hmC and 5-hmU is achieved. According to the invention, a large amount of liquid drops can be formed in a short time through a microfluidic liquid drop method; the high-throughput agarose gel microspheres wrapping the single-cell genome are efficiently obtained; thus, analysis of a high-throughput single-cell target object is realized; the difference caused by cell heterogeneity is avoided; accurate detection is realized; and the reaction has the advantages of specificity, high yield,simple reaction conditions, high reaction speed and the like.

The invention belongs to the technical field of biosensing detection, and relates to a preparation method and an application of a dual-ratio adapter sensor based on electrochemistry and photoelectrochemistry. Specifically, the invention proposes a novel dual-ratio adapter sensor based on electrochemistry and photoelectrochemistry methods for specifically detecting streptomycin (STR). The preparation method comprises the following steps: step 1, preparation of gold nanoparticles (Au NPs) and design of various nucleotide chain bases in a hybrid chain reaction; step 2, construction of the dual-ratio adapter sensor based on electrochemistry and photoelectrochemistry. The dual-ratio adapter sensor based on electrochemistry and photoelectrochemistry provides an effective way for reducing external environmental influences, and accurate and wide range detection of streptomycin.

The invention discloses a voltage controlled oscillator, which comprises a bipolar voltage controlled oscillator unit and an output buffer unit, wherein the bipolar voltage controlled oscillator unit has a difference structure and comprises an LC resonant circuit module and a negative resistance circuit module; the LC resonant circuit module is used for generating first oscillation signals of the needed oscillation frequency; the negative resistance circuit module comprises a pair of first bipolar transistors; the output buffer unit comprises two second bipolar transistors; bases of the two second bipolar transistors are respectively connected with a first output port of the LC resonant circuit module; and emitters of the two second bipolar transistors serve as two second output ports of the voltage controlled oscillator and output differential pairs of second oscillation signals. Power of the oscillation signals can be improved, phase noise of the circuit can be reduced in the condition of not changing the oscillation frequency of the oscillation signals, and online test can be carried out on radio frequency parameters of the oscillation signals on a radio frequency probe table.

The invention discloses an array fiber laser device which comprises a multi-core fiber. The multi-core fiber is a capillary tube, and multiple gain fibers and coreless fibers laid in the radial direction of the capillary tube are fixed to the inside of the multi-core fiber; two end surface and side surfaces of the capillary tube are plated with high-reflective films for pump light; film layers with high reflectivity for the pump light or photoetching gratings are applied to end surfaces of the gain fibers; the refractive index of the coreless fibers is not larger than that of an adjacent medium. The invention further discloses another array fiber laser device. The coreless fibers are used for introducing the pump light into the multi-core optical fiber, and side pumping is performed on the gain fibers in the multi-core fiber for multiple times, so that multi-wavelength signals are amplified. The invention further discloses two manufacturing methods of the multi-core fiber. The laser device or an amplifier can realize multi-wavelength laser output and signal amplification, is an all-solid-state device, and is easy to operate, simple in structure and manufacturing process and low in cost.

The invention relates to an infrared signal transmission circuit of a remote controller, which comprises an NPN type triode, wherein the collector of the NPY type triode is connected to the cathode of an infrared transmission diode; the base of the NPN type triode is connected to a carrier signal wire, while an emitter is grounded; and the anode of the infrared transmission diode is connected to a signal wire for data to be transmitted. As signal modulation and signal amplification are simultaneously realized by the NPN type triode and components are reduced, the infrared signal transmission circuit and a debugging process are simplified, and the cost is saved. In addition, the infrared signal transmission circuit generates carrier signals and signals with the data to be transmitted by the same integrated chip, so the carrier signals and the signals with the data to be transmitted are not constructed by separate elements, and the accuracy is improved.

The invention discloses a method for changing the number and state of nano magnetic particles based on a biological orthogonal reaction for detecting pesticide residues. According to the method, the number and the aggregation state of the nano magnetic particles are changed simultaneously on the basis of the cascade biological orthogonal reaction of diphenyl cyclooctyne and azide, controllable adjustment of the number and the state of the magnetic nano particles is realized, and magnetic signal cascade amplification is performed by organic combination of diphenyl cyclooctyne and azide, so thatthe accuracy and the sensitivity of pesticide residue detection are improved.

The invention provides an electrochemical sensor for detecting MicroRNA-21 and a detection system comprising the electrochemical sensor. The electrochemical detection electrode system comprises a working electrode, a reference electrode and a counter electrode, wherein the working electrode is obtained by fixing a capture probe aiming at MicroRNA-21 on the surface of a gold electrode. The MicroRNA-21 is detected by using the sensor or the detection system disclosed by the invention, the concentration of the MicroRNA-21 is in a linear relation in a range from 0.001nmol/ml to 25nmol/ml, the linear equation refers to Y=1.19999E<-5>+3.63774E<-6>X, the correlation coefficient is 0.9973, and the lowest detection limit is 0.6pmol/ml. Compared with the prior art, the sensor or the detection system is high in sensitivity, high in specificity, low in cost, easy and convenient to operate and short in detection period, is suitable for measuring the MicroRNA-21 in actual samples and is expected to become a sensor or a detection system with actual application values.

The invention discloses a new method for detecting single base difference (SBD) based on magnetic separation and a solid-phase single base circular extension (SBCE) technology. The method is characterized in that: magnetic particles are used as carriers for fixing extension primers of locus to be detected; under the action of Taq enzyme, specific fluorescent labeled ddNTP is extended to 3' end of the primers and genotype signals of a template are amplified by thermal cycling reaction which comprises denaturation, annealing and extension. The method is characterized by simplicity, preciseness, low cost, high flux and high sensitivity and automated operation can be realized by the method, therefore, the method is more practical than the traditional methods.

The invention discloses a method for detecting microRNA based on a triple-helix DNA structure, and relates to the technical field of fluorescent microRNA detection. A new triple-helix DNA structure is formed through the hybridization reaction between the three probes on the surface of the microfluidic paper chip, and the triple-helix DNA structure is cut into two parts under the action of endonuclease and exonuclease, One part can circulate and participate in the reaction to achieve signal amplification, and the other part reacts with the target microRNA to generate DNA-RNA double strands, and transfers the formed DNA-RNA double strands to the surface of another paper chip modified with MnO2 nanosheets and adds DSN to detect microRNA. Compared with traditional methods, this method has higher sensitivity, simple operation, and less time-consuming, which greatly promotes the development of a simple and rapid detection system, and helps us to quantitatively analyze microRNA and understand the physiological and pathological mechanisms of organisms. And finally provide new ideas and theoretical basis for the diagnosis and treatment of diseases.