214results about How to "Strong fluorescent signal" patented technology

The invention relates to a preparation method and an application of a magnetic fluorescent nanoparticle with a shell-core structure. Firstly, a silica magnetic microsphere with a shell-core structure is prepared by using one or more than one of nanoparticles of Fe3O4, gamma-Fe2O3, MeFe2O4 (Me=Co, Mn, Ni), metal Ni, Co, Fe, and alloy Fe-Co, Ni-Fe as the inner core, and coating a silica shell, and then a fluorescent material (a chelate of Eu3+, Sm3+, Dy3+, Tb3+ and the like) is absorbed on the silica shell. Then, a layer of silica is coated on the surface to improve the stability of the fluorescent magnetic microsphere, and to prevent agglomeration and fluorescent material leakage. A lot of rare earth fluorescent materials are wrapped in the shell layer, so the fluorescence intensity signal of a prepared sample is greatly increased. The nanoparticle has dual functions of enrichment and marking, and has wider application prospects in the biomedical field.

The invention provides a fluorescence immunochromatography test strip prepared by taking an aggregation-induced emission fluorescent microsphere as a beacon carrier. Filter paper, a sample cushion, anAIEFM (Aggregation-Induced Emission Fluorescent Microsphere) compound glass fiber cushion, a nitrocellulose membrane sprayed with a detection line and a quality control line, and water adsorption paper are overlapped and pasted to a bottom plate in sequence to prepare the fluorescence immunochromatography test strip, the fluorescence immunochromatography test strip is used for quantitatively detecting the concentration of an object to be tested in a sample, and the prepared test strip has the characteristics of being high in detection sensitivity.

The invention discloses a method for rapid optical clearing of biological tissues. The method includes: employs a strong polar aprotic solvent to conduct degreasing, wherein the strong polar aprotic solvent can be methyl methacrylate, hexamethylphosphoric triamide, N-methyl pyrrolidone, 1, 3-dimethyl-2-imidazolidinone, 1, 4-dioxane or 2, 5-dimethylfuran, then selecting a specific refractive index matching reagent with a refractive index of 1.52-1.59 to conduct clearing treatment, thus realizing rapid clearing of biological tissues. The degreasing and clearing treatment totally need only 18h to realize high clearing of mouse brain tissues, the method not only can realize brain tissue clearing, but also can achieve clearing of various organs, can well maintain the original morphology of biological tissues, and has high clearing degree and repeatability, and good fluorescence retaining effect, the soma and nerve fiber have strong fluorescence signal, and the application range is wide.

The invention relates to Ru(bpy)3-doped Ag@SiO2 fluorescent nano particles and a preparation method thereof. The fluorescent nano particles take Ru(bpy)3-doped silver as an inner core, silica with a net structure is coated on the surface of Ru(bpy)3, and an active amino group is positioned on the surface of the silica, wherein the mass ratio of the Ru(bpy)3 to silver is 1:1-1:10; and the mass ratio of the inner core to the silica is 1:5-1:12, and per milligram of nano particles contains 70-80nmol of amino. In the invention, a simple reverse microemulsion method is adopted to prepare the novelRu(bpy)3-doped core-shell type Ag@SiO2 nano particles. The nano particles have strong fluorescent signal, good light stability, good water-solubility and good biocompatibility; and as the active amino groups are positioned on the surface of the nano particles, the nano particles can directly react with biomolecules without surface modification.

The invention relates to a fluorescence nano-material, and particularly relates to a preparation method of a fluorescence probe and applications of the fluorescence probe. The fluorescence probe has characteristics of strong fluorescence signals, high stability and good water solubility. The fluorescence probe is prepared by connecting reduced carbon quantum dots capable of emitting blue fluorescence to single-stranded DNA. The reduced carbon quantum dots are prepared from carbon quantum dots, and are prepare by subjecting the carbon quantum dots to surface modification by adopting sodium borohydride as a reductant and adopting a chemical reduction method so as to obtain the reduced carbon quantum dots capable of emitting the blue fluorescence. Detection of the target DNA is achieved by utilization of fluorescence quenching-recovery processes of the fluorescence probe.

The invention relates to a near infrared fluorescence probe adopting nile blue as a parent, a preparation method thereof and applications thereof. The fluorescence probe has a structure shown as the general formula I as the attached drawing 1. The fluorescence probe can effectively improve deficiencies of tumor labeling fluorescence probes at present, can sensitively and accurately response to target cells COX-2 expression amount of which is abnormal, and is suitable for effective specific near infrared fluorescence probes labeling living tumor cells. The fluorescence probe provided by the invention is simple in synthesis and products are easy to obtain. The fluorescence probe provided by the invention has a very low fluorescence background in non-tumor cells and high fluorescence signals in tumor cells, and has strong specific labeling function for tumor cells. In addition, compounds of the type have good cell membrane permeability, low biotoxicity, low phototoxicity and low photobleaching capability and can locate a special organelle of a certain kind tumor cell.

The invention discloses a method for quickly detecting colibacillus, comprising the following steps: firstly, using anti-goat colibacillus antibody to mark functional fluorescent nanometre particle, then, adding to a sample to be detected, introducing the sample to be detected to a micro flow control chip, using a signal supply system to supply the corresponding frequency and voltage in the chip,observing and recording a fluorescent signal nearby an electrode at certain flow speed, at last, judging whether the sample to be detected has the colibacillus and judging the concentration. The usedmicro flow control chip comprises a base sheet and a cover sheet which are adhered together; a capillary passage is assembled between the base sheet and the cover sheet; the two ends of the passage respectively are respectively connected with a sample inlet and a sample outlet on the cover sheet; and the passage is internally provided with paired platinum electrodes. The method has the advantagesof good specificity, high flexibility, low cost, quick speed, strong selectivity and suitability.

The invention relates to application of a fluorescent in-situ hybridization polyclonal separating probe for renal carcinoma and a kit thereof. Clonal fragments used by the polyclonal separating probe provided by the invention are respectively a combination of RP11-416B14, CTD-2522M13 and CTD-2516D6, and a combination of CTD-2312C1, CTD-2248C21 and RP11-959H17. According to the invention, the defect that karyotype analysis can not be performed conventionally after tumor cells are subjected to cell culture until a mitotic phase in the prior art is overcome. The typical gene modification in Xp11.2 translocation / TFE3 gene fusion related renal carcinoma is detected by using the FISH technology, thereby diagnosing the tumor. The fluorescent in-situ hybridization polyclonal separating probe application is high in accuracy, high in specificity, high in success rate, strong in fluorescent signals and convenient to operate. The invention can be applied in paraffin sections, widens the scope of detection specimens, and greatly optimizes the diagnosis method of Xp11.2 translocation / TFE3 gene fusion related renal carcinoma.

The invention provides an easily-modified near-infrared region II organic small molecule dye and a synthetic method and application of the easily-modified near-infrared region II organic small molecule dye. The near-infrared region II fluorescent dye belongs to polymethenyl pyran or polymethenyl thiapyran salt organic small molecules, and is prepared by a condensation reaction of polymethenyl aniline salt with pyran or thiopyran salt. The synthetic raw materials are easy to obtain, the cost is low, the preparation process is simple, the yield is high, and large-amount synthesis can be achieved. The purpose of adjusting the fluorescence emission spectrum is achieved by changing an intramolecular polymethenyl chain and hetero atom species. The small molecule dye has the maximum emission wavelength range of 1000-1200 nm, and is high in fluorescence quantum yield, good in light stability and quite applicable to living imaging. The small molecule dye can be linked to groups with specific functions by click chemistry for a variety of bioimaging application. Near-infrared region II fluorescent living imaging achieves low background noise, strong fluorescence signal and high signal-to-noise ratio.

The invention relates to an ASPL-TFE3 fused renal carcinoma gene probe as well as an application of a kit thereof. Cloned fragments selected by the gene probe are respectively a combination of RP11-634L10, RP11-51H16 and RP11-475F12 and a combination of CTD-2311N12, RP11-416B14, CTD-2522M13, CTD-2516D6, CTD-2312C1, CTD-2248C21 and RP11-959H17. By using the ASPL-TFE3 fused renal carcinoma gene probe, the defects of complexity, time consumption and clinic application unsuitability of RT-PCR and cell karyotyping methods applied in the past are overcome. According to the ASPL-TFE3 fused renal carcinoma gene probe, a unique ASPL-TFE3 fused gene in ASPL-TFE3 fused renal carcinoma is detected by using an FISH technology, so that the renal carcinoma is diagnosed; the gene probe is high in accuracy rate, specificity and success rate, strong in fluorescence signal, simple and rapid in operation and rapid in diagnosis when applied and can be applied to paraffin section; and due to the application of the ASPL-TFE3 fused renal carcinoma gene probe, the specimen detecting range is widened, a novel method for accurately, reliably, simply and conveniently diagnosing the ASPL-TFE3 fused renal carcinoma is established, and the precedent that the ASPL-TFE3 fused renal carcinoma is detected by virtue of FISH is created.

The invention belongs to the technical field of bacteria detection, and more specifically discloses a group B streptococcus fluorescent quantitative PCR detection kit. The group B streptococcus fluorescent quantitative PCR detection kit comprises a group of group B streptococcus fluorescent quantitative PCR detection primers and probes; the primers and probes comprise GBS amplification primer pairGBS-F and GBS-R, GBS detection probe GBS-P, internal reference primer pair beta-actin-F and beta-actin-R, and internal reference probe beta-actin-P; the nucleotide sequences of GBS-F, GBS-R, GBS-P, beta-actin-F, beta-actin-R, and beta-actin-P are represented by SEQ ID NO:1-6 respectively. The group B streptococcus fluorescent quantitative PCR detection kit can be used for detecting GBS in samplewith complex composition, and detecting single copy templates, is wider in linearity range, is capable of avoiding false positive results caused by amplification products pollution, and is high in sensitivity and specificity.

The invention discloses a fluorescence detection method for detecting cocaine by use of oligonucleotide and graphene oxide. The method comprises the following steps of: adding a dye-marked oligonucleotide probe into a buffer solution; adding graphene oxide as a solid-phase carrier so as to adsorb the oligonucleotide probe onto the surface of the graphene oxide and form an oligonucleotide / graphene oxide compound, and quenching a fluorescence signal of the dye; adding a complementary probe for reaction, wherein a double-strand secondary structure is formed through the base combination effect between the complementary probe and the oligonucleotide probe so as to recover fluorescence; and adding cocaine for reaction, wherein three stem-like secondary structures are formed through the specific effect between the cocaine and the complementary probe so that the oligonucleotide probe is re-combined to the surface of the graphene oxide and the fluorescence is quenched. According to the invention, the aim of cocaine detection is achieved by detecting the change of fluorescence intensity in the solution; and the detection method is simple and quick, and has relatively high specificity and sensitivity.

The invention provides a time-resolved immunofluorometric assay detection kit for enrofloxacin residues and a detection method thereof. The detection kit is coated with the elisa plate of an enrofloxacin antigen, and a lanthanide is marked with a goat anti rabbit or goat anti mouse antibody, an enrofloxacin antibody and the like. The invention also discloses a method applying the above detection kit to detect enrofloxacin residues. In the detection kit for enrofloxacin residues provided by the invention, the indirect competitive time immunofluorometric assay technology is adopted, so that the detection kit has the advantages of high sensitivity and good stability, is very suitable for screening a great quantity of samples and has an important practical meaning; and operation steps is greatly simplified, reaction time is greatly shortened, errors caused by complex operation are reduced, and cost is lowered.

The invention discloses a rhodamine B derivative containing an 8-aminoquinoline group, a preparation method, an application and a method for carrying out fluorescence analysis on Cr<3+> and Cu<2+>. The rhodamine B derivative containing the 8-aminoquinoline group is capable of selectively and quantitatively detecting chromium ions (Cr<3+>) in acetonitrile, and is capable of selectively and quantitatively detecting copper ions (Cu<2+>) in ethanol and generating an obvious fluorescence increment effect. The rhodamine B derivative containing the 8-aminoquinoline group is simple in synthesis method, and is capable of quantitatively detecting different heavy metal ions Cr<3+> and Cu<2+> in different solvents at high selectivity and high sensitivity, is a dual-functional fluorescence probe, and has a good application prospect in the fields of environment analysis and detection.

The invention relates to a triple-amplification-technology-based homogeneous-phase label-free method for detecting the activity of telomerase. The method is characterized in that a telomerase extension product is combined with accessory DNA to release triggering DNA, and the triggering DNA can trigger a strand displacement reaction to form a hairpin H1:H2 compound; G-quadruplexes can be formed at the two tail ends of the compound respectively, fluorescence signals of NMM are weak and are remarkably enhanced after the G-quadruplexes are embedded and inserted, and the activity of the telomerase can be sensitively detected by detecting changes of the fluorescence signals of the NMM. According to the method, the strand displacement reaction is sufficiently adopted, and signal amplification can be achieved without assisting of enzymes; meanwhile, formation of the G-quadruplexes is ingeniously adopted, the method in which the activity of the telomerase can be sensitively detected without labeling is constructed, a new concept is provided for diagnosis and treatment of tumor diseases and researching of disease mechanisms, and the method is of great significance in the telomerase-based cancer treatment and diagnosis aspect.

The invention relates to a graphene quantum dot sensor and its application in detection of trinitrophenol, and belongs to the field of analysis chemistry. A making method of the sensor comprises the following steps: mixing citric acid with ammonia water, and heating in a closed microwave digestion device to prepare nitrogen element doped graphene quantum dots; mixing the graphene quantum dots with a carboxymethyl chitosan solution, immersing a quartz thin plate in the obtained graphene quantum dot and carboxymethyl chitosan solution, and carrying out electrostatic force action to form a thin plate with a strong fluorescence signal. Creatinine molecules are adsorbed to the surface of the thin plate to identify trinitrophenol. When the thin plate is in contact with trinitrophenol, the fluorescence signal substantially weakens. The graphene quantum dot fluorescence thin plate chemical sensor made through the method has good photochemical stability, has obvious selection and identification capability on the explosive trinitrophenol, can realize detection of a tiny amount of trinitrophenol (the detection limit is 0.1[mu]g / L), and has wide application prospects in safety detection and environmental protection supervision.

The invention provides large stokes displacement fluorescent protein CyOFP and application of the fluorescent protein to microimaging and highly-sensitive living creature light-emitting imaging. The fluorescent protein is obtained through mNeptune site directed mutagenesis, the 1-230 positions of the amino acid sequence of the fluorescent protein sequentially correspond to 4-234 positions shown by the SEQ ID No: 2, or the fluorescent protein can be further obtained by conducting gene synthesizing on the fluorescent protein DNA segment and expression. The invention further provides a novel BRET system and fusion protein Antares obtained through system optimization. The CyOFP can be stimulated by blue light, and a quite high quantum yield is achived; the CyOFP and the enhanced green fluorescent protein (EGFP) are jointly stimulated by monochromatic light at the same time in a two-photon mode, and the resolution ratio of two-photon imaging is raised; a novel BRET system formed together with fluorescent protease NanoLuc greatly improves the depth and sensitivity of living creature imaging.

The invention provides a method for preparing a ZnS quantum dot modified by mercaptoacetic acid, belonging to the technical field of ZnS nano material preparation. The ZnS quantum dot modified by mercaptoacetic acid is synthesized in one step by using a hydro-thermal reaction method in an aqueous solution based on mercaptoacetic acid as a modifier. The method has the advantages that the synthesized ZnS quantum dot modified by mercaptoacetic acid has uniform in size, strong fluorescent signals and good dispersibility in water, and can form stable hydrosol; a carboxyl group connected to the surface of the ZnS quantum dot can be connected with a large biological molecule, which is suitable for detection of biological substances; and in addition, compared with other methods for preparing the ZnS quantum dot modified by mercaptoacetic acid, in the method provided by the invention, reaction and modification links are completed in one step, so that preparation process is simple and cost is low.

The present invention belongs to the field of fluorescent proteins in biotechnology, and particularly relates to a preparation method of a high fluorescence intensity recombinant phycobiliprotein concatermer. According to the preparation method, a streptavidin gene is linked to an allophycocyanin alpha subunit gene through a linker sequence; on the basis, one or a plurality of allophycocyanin alpha subunit genes are connected in series through linker sequences to form a fusion gene; and the fusion gene, a phycobiliprotein lyase gent and a phycoerythrobilin biosynthetic enzyme gene co-express in Escherichia coli to obtain the recombinant allophycocyanin concatermer with characteristics of biotin binding ability and high fluorescence intensity. According to the present invention, tn the immunofluorescence assay, the recombinant phycobiliprotein concatermer can achieve the strong fluorescent signal compared to the recombinant phycobiliprotein monomer; and the prepared recombinant phycobiliprotein concatermer can be adopted as the fluorescent marker for immunofluorescence detection in the field of biology and biomedicine.

The invention discloses a fluorescence resonance energy transfer polystyrene fluorescent microsphere. Two fluorescent materials such as rhodamine B n-octyl ester and nile blue A are embedded in polystyrene microspheres, and by virtue of fluorescence resonance energy transfer, the purpose of prolonging the Stockton displacement is achieved. Exciting light and emitted light can be easily distinguished, and interference of the exciting light on detection and interference on a fluorescence signal in a sample can be reduced.

The invention discloses a lanthanide high-sensitivity fluorescence chromatography device and a detection method. The device comprises an excitation light source, a mobile device, a beam splitter, a light source light path, a fluorescence light path, a photoelectric converter, a circuit device and a communication device, wherein the mobile device comprises a stepper motor and a transmission mechanism driven by the stepper motor; the transmission mechanism is provided with a chromatography detection card; the excitation light source is arranged corresponding to the chromatography detection card; the beam splitter is arranged between the excitation light source and the chromatography detection card; the light source light path is arranged between the beam splitter and the chromatography detection card; the fluorescence light path is arranged between the beam splitter and the photoelectric converter; the excitation light source, the photoelectric converter and the mobile device are controlled by the circuit device; the excitation light source is capable of emitting excitation light of which the wavelength is greater than 400nm; the circuit device drives the communication device; and the communication device is connected with a terminal. According to the lanthanide high-sensitivity fluorescence chromatography device and the detection method, low background fluorescence is ensured, and a high fluorescence signal is ensured so as to improve the signal to noise ratio.

The invention relates to the field of nano materials and novel quantum dots, and particularly provides a silicon core quantum dot shell composite nano material, a preparation method, application and aproduct. The composite nano material provided by the invention has a core-shell structure, an inner core layer is SiO2 nano particles, an outer shell layer is carboxylated quantum dots, and an electropositive polymer layer is also arranged between the inner core layer and the outer shell layer. The preparation method comprises the following steps: firstly, modifying the surfaces of SiO2 nanoparticles with an electropositive polymer, and then adsorbing a layer of carboxylated quantum dots to form the composite nano material with the silicon core quantum dot shell. The composite nano material is good in dispersity, adjustable in particle size, high in fluorescence intensity and easy to prepare in batches, and can be used for capturing and high-sensitivity detection of target substances in complex samples.

A two-photon fluorescent dye taking isoquinolinone as the parent is provided with a structure presented in formula I, wherein R1 is selected from -H, -CN, -OCH3, -COOH, -OH, -NO2, -NH2, -NHCH3, -N(CH3)2, -OCOCH3 and -SH; R2 is selected from -N-, -C-, -S- and -O-; and R3 is selected from -NHCH2OH, -NH(CH2)2OH, -NH(CH2)3OH, -NH(CH2)4OH,-N(CH3)CH2OH, - N(CH3)(CH2)2OH, -N(CH3)(CH2)3OH and -N(CH3)(CH2)4OH. The fluorescent dye is efficient in two-photon absorptive capacity, low in photo-induced toxicity and photobleaching, and strong in specificity for ribonucleic acid (RNA) in cells; and can perform specificity marking on the RHA in living cells and fixed cells simultaneously. The formula I is shown in the description.

The invention discloses a genetic engineering biological indicator used for biological monitoring of one or more sterilization effects. According to the genetic engineering biological indicator, foreign genes, including promoters, reporter genes and selectable maker genes, are guided into indicator microorganisms by means of genetic engineering. The selectable maker genes are positioned on the upstream of the inducible promoters, and the reporter genes are positioned on the downstream of the inducible promoters. In the absence of inducers, the selectable maker genes are expressed, the reporter genes are not expressed, and accordingly, successfully-transfected strains are screened. After exposure by sterilization factors, the indicator microorganisms contact with the inducers, and the reporter genes are expressed. Autofluorescence of the genetic engineering biological indicator can be achieved without specific enzyme substrates, and the genetic engineering biological indicator has the advantages of high signal-to-noise ratio, strong fluorescence signal, easiness in detection, high sensitivity and the like.

The invention discloses a cardiopulmonary quintuplet detection kit , a rare earth nano fluorescence detection card thereof and a detection method thereof. The content of the cTnI / CKMB / Myo / D-Dimer / NT-proBNP in human blood can be accurately and quantitatively detected; a rare earth nano fluorescent microsphere detection technology is used; fluorescence interference of a sample can be avoided; the fluorescent microsphere is a rare earth fluoride near-infrared two-region light-emitting nano material and has a low background; the microsphere is connected with the antibody by virtue of a covalent bond, so that a labeled product is stable, has the characteristics of wide detection range, high sensitivity, high accuracy, rapidness, simplicity and convenience in detection and the like, and can helpdoctors to accurately diagnose the cardio-pulmonary function in the early stage.

The invention relates to a near-infrared second region fluorescent nanoprobe based on black phosphorus as well as a preparation and application of the near-infrared second region fluorescent nanoprobe. The preparation method of the near-infrared second region fluorescent nanoprobe comprises the following steps: uniformly mixing red phosphorus or black phosphorus with a ball-milling body and then carrying out ball milling for 1 to 200 hours; then adding a hydrophobic ligand into a mixture and continuously carrying out ball milling for 1 to 200 hours to obtain black phosphorus nanoparticles, thesurfaces of which are modified with the hydrophobic ligand; dissolving the black phosphorus nanoparticles modified with the hydrophobic ligand on the surface and the amphipathic molecules into a volatile organic solvent according to the mass ratio of 1 to (100 to 200); then volatilizing to remove the organic solvent, uniformly mixing the obtained substance with water and vigorously stirring to obtain water-soluble near-infrared second region fluorescent nanoprobe based on the black phosphorus. The near-infrared second region fluorescent nanoprobe obtained by the preparation method disclosed by the invention has the advantages of stronger and wider fluorescent signal, capability of achieving multi-wavelength excitation and multi-wavelength emission and broad application prospect in biological imaging.

The invention relates to a 25-hydroxyvitamin D TRFIA (Time-Resolved Fluoroimmunoassay) kit and a detection method, which utilize the sensitivity of TRFIA technology, utilize rare earth vanadate nano fluorescent labeling materials and combine a dry-type immunofluorescence analyzer to realize high sensitivity, accurate quantification, simpleness and convenience. The kit of the invention can accurately and quantitatively detect the content of the 25-hydroxyvitamin D in serum, plasma and whole blood samples of persons, the whole blood samples can be filtered through the treatment of blood filtering membrane sample pads, the fluorescence interference of the samples can be avoided by utilization of a time resolution detection technology, the fluorescent microspheres are rare earth vanadate nanomaterials, thus the advantages of low background, strong fluorescence signals, high signal-to-noise ratio and the like are achieved, rare earth vanadate nano particles are connected with antibody through covalent bonds by labelling, a labeled product is stable, thus the kit has the characteristics of wide detection range (3-100ng / mL), high sensitivity (detection limit is 3ng / mL), high accuracy, quick and convenient detection and the like.