Lanthanide high-sensitivity fluorescence chromatography device and detection method

Abstract

The invention discloses a lanthanide high-sensitivity fluorescence chromatography device and a detection method. The device comprises an excitation light source, a mobile device, a beam splitter, a light source light path, a fluorescence light path, a photoelectric converter, a circuit device and a communication device, wherein the mobile device comprises a stepper motor and a transmission mechanism driven by the stepper motor; the transmission mechanism is provided with a chromatography detection card; the excitation light source is arranged corresponding to the chromatography detection card; the beam splitter is arranged between the excitation light source and the chromatography detection card; the light source light path is arranged between the beam splitter and the chromatography detection card; the fluorescence light path is arranged between the beam splitter and the photoelectric converter; the excitation light source, the photoelectric converter and the mobile device are controlled by the circuit device; the excitation light source is capable of emitting excitation light of which the wavelength is greater than 400nm; the circuit device drives the communication device; and the communication device is connected with a terminal. According to the lanthanide high-sensitivity fluorescence chromatography device and the detection method, low background fluorescence is ensured, and a high fluorescence signal is ensured so as to improve the signal to noise ratio.

Description

Technical field [0001] The invention belongs to the technical field of chromatography detection, and particularly relates to a lanthanide series high-sensitivity fluorescence chromatography device and a detection method. Background technique [0002] In order to meet the application of POCT point-of-care testing, the gold standard chromatography rapid detection method is widely used. With the demand for quantitative detection, various microspheres that replace colloidal gold have appeared in the market, mainly In the fluorescent beads and time-resolved beads. [0003] Ordinary fluorescent microspheres have a strong fluorescent signal, which greatly improves the detection signal intensity of the chromatography strip, and solves the problem that the color judgment of colloidal gold cannot be subdivided. The time-resolved fluorescence has high specificity. The method of delaying the collection of fluorescence signals at the time of irradiating the fluorescent microspheres by avoiding...

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Problems solved by technology

[0004] How to solve the contradiction between the large background interference of ordinary fluorescence and the weak time-resolved fluorescence signal has become the direction of efforts of technicians in this field

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