48 results about "Fluorescence tomography" patented technology

In a fluorescence tomography apparatus for obtaining fluorescence tomographic images of a region of an object to be observed: an application unit concurrently applies an ultrasonic wave and excitation light to the region so that ultrasound-modulated fluorescence is emitted from the region under the influence of the ultrasonic wave; and a first image acquisition unit acquires a fluorescence tomographic image of the region on the basis of the ultrasound-modulated fluorescence.

Methods and apparatus for medical imaging using diffusive optical tomography and fluorescent diffusive optical tomography are disclosed. In one embodiment, a method for medical imaging comprises, scanning a tissue volume with near-infrared light to obtain structural parameters, wherein the tissue volume includes a biological entity, scanning the tissue volume with near-infrared light to obtain optical and fluorescence measurements of the scanned volume, segmenting the scanned volume into a first region and a second region, and reconstructing an optical image and a fluorescence image of at least a portion of the scanned volume from the structural parameters and the optical and fluorescence measurements. In another embodiment an apparatus for medical imaging is disclosed.

The invention discloses a living small animal imaging system in a miniature CT and fluorescent tomography dual mode and an imaging method thereof. The living small animal imaging system in the miniature CT and the fluorescent tomography dual mode comprises a main control computer, an X-ray source and an X-ray detection device, an excitation source and an excitation light / fluorescent light detection device, and a rotary scanning device, wherein the X-ray source and the X-ray detection device, the excitation source and the excitation light / fluorescent light detection device, and the rotary scanning device are controlled by the main control computer. The invention can simultaneously acquire structure information by carrying out the miniature CT imaging on a small animal and fluorescent-labeled molecular information by carrying out the fluorescent tomography on small animals to enable the molecular information acquired by the fluorescent tomography to be accurately positioned in the smallanimal, thereby being beneficial to improving the diagnosis accuracy.

The invention discloses a photoacoustic and fluorescence dual-mode integrated tomography imaging system and a photoacoustic and fluorescence dual-mode integrated tomography imaging method. The system comprises a modulation laser light source (1), a vibrating mirror (2), a sample rotary table (3), a filtering wheel (4), a gating intensified charge-coupled device (ICCD) camera (5), a photoacoustic detection acquisition module (6), a system control module (7), a computer (8), a data bus (9) and a control bus (10), wherein the vibrating mirror, the sample rotary table, the filtering wheel and the gating ICCD camera are positioned on the same straight line with the direction of lasers; the ultrasound transduction array normal direction of the photoacoustic detection acquisition module is perpendicular to the straight line to form a crossed vertical detection mode. According to the imaging method, light absorption distribution formed by photoacoustic tomography imaging is used as prior information for the reestablishment of fluorescence tomography imaging, and the positions of fluorophores, fluorescent yield and fluorescence lifetime parameters are reestablished by an iterative method. By the photoacoustic and fluorescence dual-mode integrated tomography imaging system and the photoacoustic and fluorescence dual-mode integrated tomography imaging method, the imaging complexity can be reduced, the cost of hardware can be saved, and the flexibility of the system and the reestablishing accuracy of the fluorescence tomography imaging can be improved.

Methods and apparatus for medical imaging using diffusive optical tomography and fluorescent diffusive optical tomography are disclosed. In one embodiment, a method for medical imaging comprises, scanning a tissue volume with near-infrared light to obtain structural parameters, wherein the tissue volume includes a biological entity, scanning the tissue volume with near-infrared light to obtain optical and fluorescence measurements of the scanned volume, segmenting the scanned volume into a first region and a second region, and reconstructing an optical image and a fluorescence image of at least a portion of the scanned volume from the structural parameters and the optical and fluorescence measurements. In another embodiment an apparatus for medical imaging is disclosed.

The present invention relates to a rotary type diffusion fluorescence tomography system. Said system includes the following several portions: semiconductor laser, computer, rotary working table, stepping motor and CCD camera. Said invention also provides its working principle and concrete operation method.

In a fluorescence tomography apparatus for obtaining fluorescence tomographic images of a region of an object to be observed: an application unit concurrently applies an ultrasonic wave and excitation light to the region so that ultrasound-modulated fluorescence is emitted from the region under the influence of the ultrasonic wave; and a first image acquisition unit acquires a fluorescence tomographic image of the region on the basis of the ultrasound-modulated fluorescence.

An exemplary method, system, and computer-accessible medium can be provided for generating three-dimensional image information associated with a fluorescence-exhibiting arrangement within a sample. For example, it is possible to generate a first electro-magnetic radiation, to be received in the sample, at one or more wavelengths that are associated with one or more wavelengths of emission of the fluorescence-exhibiting arrangement. In addition, it is possible to generate a second electro-magnetic radiation from the sample which is caused by the fluorescence-exhibiting arrangement in response to the first electro-magnetic radiation, and generate data associated with the second electro-magnetic radiation. Then, it is possible to receive the data, and generate three-dimensional information based on the data.

An apparatus for providing an integrated tri-modality system includes a fluorescence tomography subsystem (FT), a diffuse optical tomography subsystem (DOT), and an x-ray tomography subsystem (XCT), where each subsystem is combined in the integrated tri-modality system to perform quantitative fluorescence tomography with the fluorescence tomography subsystem (FT) using multimodality imaging with the x-ray tomography subsystem (XCT) providing XCT anatomical information as structural a priori data to the integrated tri-modality system, while the diffuse optical tomography subsystem (DOT) provides optical background heterogeneity information from DOT measurements to the integrated tri-modality system as functional a priori data. A method includes using FT, DOT, and XCT in an integrated fashion wherein DOT data is acquired to recover the optical property of the whole medium to accurately describe photon propagation in tissue, where structural limitations are derived from XCT, and accurate fluorescence concentration and lifetime parameters are recovered to form an accurate image.

The invention discloses an up-conversion fluorescence tomography test paper detection result reading device based on a mobile terminal, and belongs to the technical field of biological detection devices. A portable laser source generation device emits laser to excite an imaging light path to be perpendicular to the front surface of an up-conversion fluorescence tomography test paper strip to be detected; the laser is focused on the up-conversion fluorescence tomography test paper to be detected through a focusing lens I to excite a quality control band and a detection band on the up-conversion fluorescence tomography test paper to be detected to emit fluorescence simultaneously; the emitted fluorescence is reflected to an upper imaging light path through a dichroscope; after the fluorescence is amplified and imaged by a focusing lens II, exciting light of 980 nm is filtered out through a filter, and the mobile terminal can shoot the light to realize result reading. The up-conversion fluorescence tomography test paper detection result reading device disclosed by the invention is simple in structure, small in size, convenient to operate and easy to carry, and can be used by a family, the community or a Census institution for quickly reading the up-conversion fluorescence tomography test paper.

A fluorescence optical tomography system and method uses a photon migration model calculator for which absorption and reduced scattering coefficient values are determined for each source / detector pair. The coefficient values may be determined by measurement, in which a time resolved detector detects the excitation wavelength and generates temporal point spread functions from which the coefficient values are found. Alternatively, the coefficient values may be determined by calculating them from a dataset containing a spatial distribution of absorption and reduced scattering coefficients in a volume of interest. The fluorescence detection may be continuous wave, time resolved, or a combination of the two. An estimator uses a detected fluorescence signal and an estimated fluorescence signal to estimate the image values.

The invention provides a three-dimensional reconstruction method of excited fluorescence tomography based on deep learning. The method comprises the following steps: S1, generating a training sample;S2, setting a deep learning model, and constructing that deep learning model comprising a picture information coding stage, a picture information fusion stage and a three-dimensional reconstruction stage; and S3, training the deep learning model, inputting the data of the organism into the deep learning model after training, and obtaining the three-dimensional reconstruction image of the organism.Based on statistical learning, the method trains the forward and reverse processes of photon propagation, and improves the precision and speed of three-dimensional reconstruction of biologically excited fluorescence computed tomography.

Systems and methods for near-infrared fluorescence (NIRF) imaging and frequency-domain photon migration (FDPM) measurements. An optical tomography system includes a bed, a wheel, a light source, an image detector, and radio frequency (RF) circuitry. The bed is configured to support an object to be imaged. The wheel is configured to rotate about the bed. The light source is coupled to the wheel. The image detector is coupled to the wheel and disposed to capture images of the object. The RF circuitry is coupled to the light source and the image detector. The RF circuitry is configured to simultaneously generate a modulation signal to modulate the light source, and generate a demodulation signal to modulate a gain of the image detector.

The invention relates to the field of small animal imaging, solves the problems that in the prior art, the image resolution is low and the image acquisition time is long, provides a photon counting-type dynamic diffusion fluorescence tomography system which has high sensitivity and image resolution and achieves the purpose of dynamic diffusion fluorescence tomography with short measurement time. In order to achieve the purpose, the invention adopts the technical scheme as follows: the photon counting-type dynamic diffusion fluorescence tomography device comprises a light source system used for fluorescence excitation, an imaging chamber system used for fixing and moving a detected object, a light switch used for switching a detection passage, a light-filtering system used for filtering off excited light signals, a detection system used for detecting light signals, and a computer used for integrated control of an attenuator, the light switch, a motor-driven light filtering wheel, a photomultipler tube and a photon counting module as well as image reconstruction. The invention is mainly applied to small animal imaging.

Aspects of the disclosure relate to fluorescence imaging, and more particularly to the use of temperature modulation in a tissue site with a modulator such as high intensity focused ultrasound to modulate the emission signal emitted by temperature-sensitive optical fluorescence reporters.

The invention provides an excited fluorescence tomography method based on a multilayer perceptual network. The method comprises the following steps: S1, generating a training sample; S2, setting a meshless standardized model and mapping the training samples into the meshless standardized model; S3, constructing a multi-layer perceptual network of excited fluorescence tomography according to a meshless standardized model, wherein that multi-layer perceptual network comprises an input layer, a hidden lay and an output layer; S4, training the meshless standardized model according to the output result of the output layer; and S5, inputting the data of the organism into the trained meshless standardized model to obtain a reconstructed image of the organism.

The invention provides a near-infrared two-region fluorescence tomography system. The system comprises a structure information acquisition module which emits an X-ray beam, and acquires a spatial structure image of a sample, a light source module which emits white light and laser, and respectively obtains a white light image and a fluorescence image after the sample is illuminated, an optical information collecting module which acquires the white light image and the fluorescent image and a central control module which controls the structure information acquisition module, the light source module and the optical information collecting module; the spatial structural image, the white light image and the fluorescent image are read, and a near-infrared two-region fluorescence tomography image of the sample is obtained according to the spatial structure image, the white light image and the fluorescence image.

A fluorescence optical tomography system and method uses a photon migration model calculator for which absorption and reduced scattering coefficient values are determined for each source / detector pair. The coefficient values may be determined by measurement, in which a time resolved detector detects the excitation wavelength and generates temporal point spread functions from which the coefficient values are found. Alternatively, the coefficient values may be determined by calculating them from a dataset containing a spatial distribution of absorption and reduced scattering coefficients in a volume of interest. The fluorescence detection may be continuous wave, time resolved, or a combination of the two. An estimator uses a detected fluorescence signal and an estimated fluorescence signal to estimate the image values.

The invention discloses a CT (computed tomography) / FT (fluorescence tomography) / PET (position emission tomography) tri-modal synchronous imaging data acquiring system. The CT / FT / PET tri-modal synchronous imaging data acquiring system comprises a support device, a power device, a signal transmission device, a data acquiring device and a control system. During use, an examination bed is disposed in an imaging hole of the data acquiring device; a motor is controlled to operate according to a required mode by a control signal sent by a computer, and simultaneously, an X-ray tube, a laser, a CCD (charge coupled device) camera and a filter wheel are indirectly controlled by a control box; a disc rack surrounds a central line of the imaging hole to rotate around a small animal according to the set mode and acquires tri-modal data; acquired data are directly stored in the computer via a slip ring; the computer respectively processes the tri-modal data and rebuilds tri-modal images, and the tri-modal images are integrated into an image displaying an examined portion and functional information of the small animal via a specific processing algorithm. The tri-modal synchronous imaging data acquiring system integrates CT, optical and PET imaging modes into the same frame structure, so that three devices are controlled to acquire data by the same control system, and integration of the tri-model images is realized.

System and method for optical tomographic imaging optimizing analysis of time-domain data as a result of combination of lifetime multiplexing of low-cross-talk asymptotic photons with highly spatially resolved early photons. The tomographic data reconstruction employs a decay amplitude-based asymptotic approach and a matrix equation with a weight matrix that includes two different portions respectively representing time domain sensitivity functions and continuous-tomography weight matrices. System may employ a lifetime fluorescent tomography imaging system.

The invention belongs to the technical field of optical molecular imaging, particularly relates to an elastic network excited fluorescence tomography reconstruction system, method and device based onself-adaptive parameter search, and aims to solve the problems of over-sparse, over-smooth, discontinuous space and the like of a region easily caused by solving tumor distribution by a single regularization constraint. The system comprises a data acquisition module which is configured to acquire biological CT three-dimensional tissue structure data and body surface excitation fluorescence image data thereof; a data segmentation and discretization module which is configured to perform organ segmentation and finite element discretization on the tissue structure data; a data fusion module whichis configured to obtain biological body surface excitation fluorescence light intensity distribution information; a model establishing module which is configured to establish a linear mathematical model; a target function generation module which is configured to generate a target function; and a search iteration and output module which is configured to calculate the effective solution of the target function, obtain the convergence distribution condition of the probes and output the convergence distribution condition. According to the method, the problem caused by solving tumor distribution through single regularization constraint is solved.

The invention discloses multimode 3D fluorescence tomography animal molecular imaging scanning equipment, and belongs to the technical field of optical molecular imaging. The multimode 3D fluorescencetomography animal molecular imaging scanning equipment comprises a light sheltering camera bellows, a sample platform mounted in the light sheltering camera bellows and a computer terminal for controlling the equipment and imaging, wherein the sample platform comprises a transparent platform which is supported by a supporting column; the outer wall of the transparent platform is rotatably connected with a rotating platform through a bearing; the rotating platform is driven by a driving mechanism to rotate; a sample box is placed on the surface of the transparent platform; a CT scanning moduleis placed on the surface of the rotating platform; a CCD camera and an LED array are arranged on the inner wall of the top end of the light sheltering camera bellows; a lens collimator is arranged right below the sample platform, and is connected with a laser through an optical fiber wire; and an FMT scanning module consists of the CCD camera, the lens collimator and the laser. According to the multimode 3D fluorescence tomography animal molecular imaging scanning equipment disclosed by the invention, the CT scanning module is combined with the FMT scanning module, so that dualmode imaging isperformed on live small animals, so that the imaging is enriched.

The invention belongs to the field of digital images, particularly relates to an optical tomography method and system based on a K-nearest neighbor local connection network, and aims to solve the problem that the reconstruction precision and imaging speed of existing FMT imaging cannot be considered at the same time. The method comprises the following steps: constructing a first sample by utilizing Monte Carlo simulation; utilizing the first sample to realize expansion of a training sample set in a sample combination mode; for the constructed K-nearest neighbor local connection network, training by using the training sample set, and optimizing a local connection sub-network by using residual learning; and performing internal light source reconstruction based on an appearance fluorescence image of the target object by using the trained K-nearest neighbor local connection network. According to the neural network method based on data driving, the reverse propagation process of photons ina living body is directly learned, and accurate and rapid excitation of fluorescence tomography is achieved.

The invention belongs to the field of medical optical coherent tomography technologies, and relates to a method for extracting information of a fluorescence tomography system for small animals. The method includes calibrating the tomography system and positioning the outer edge of a tomography cavity in a focal plane of a lens; respectively drawing luminous intensity distribution curves of emergent light of excitation light and fluorescence; selecting a range of a central angle theta of the tomography cavity and an information extraction region S mapped to a detected image; individually utilizing pixel data corresponding to a range of small luminous intensity values in the information extraction region S as a sub-region according to the luminous intensity distribution curves of the emergent light, and respectively utilizing pixel data corresponding to two-side ranges of high luminous intensity values as two sub-regions; solving an average value of measured data in each sub-region, utilizing each average value as an average detection value and reconstructing the image by the aid of all the extracted average detection values. The method has the advantages that computation can be simplified, and influence of two-side stray light can be eliminated.

The invention discloses a fluorescence control method of a light-controlled fluorescent protein marker biological tissue embedding sample. Activation light is used for activating the light-controlled fluorescent protein on the surface layer of the sample, and exciting light is used for exciting the light-controlled fluorescent protein on the surface layer to emit fluorescence. The method comprises the following steps: selecting appropriate wavelength ranges of the activation light and the exciting light, and controlling an appropriate included angle between the activation direction and the upper surface of the sample at the same time, thereby realizing the precise control of the surface layer fluorescence of the light-controlled fluorescent protein marker biological tissue embedding sample, wherein the activation is convenient, and the thickness of the activated surface layer is thin. Only the fluorescence on the surface layer of the biological tissue embedding sample is activated when the fluorescence control method disclosed by the invention is used for activating; the exciting light excites the activated surface layer to perform fluorescence imaging when the method is applied to the fluorescent tomography of the biological tissue embedding sample, the thickness of the activated surface layer of the sample is the axial resolution of the fluorescence imaging, and the axial resolution is high.

The invention belongs to the field of biomedical molecular imaging, particularly relates to an excitation fluorescence tomography method, system and equipment based on a GCN residual connection network, and aims to solve the problems of model precision reduction, reconstruction precision reduction and slow reconstruction speed in the traditional FMT reconstruction based on a photon propagation model. The method comprises the following steps: meshing segmented CT image data of an organism, and carrying out graph structure modeling; simulating a photon propagation process of an in-vivo light source in the organism to obtain fluorescence distribution on the surface and in the organism, and expanding the fluorescence distribution as a light source sample; constructing a first node set; inputting the expanded light source sample and each node in the first node set into a deep learning network model, and training the model; and utilizing the trained deep learning network model to carry out excitation fluorescence tomography reconstruction on the organism. According to the invention, excitation fluorescence tomography with high reconstruction quality and high reconstruction speed is realized.

The invention discloses a rotary diffusion fluorescence tomography imaging system, which comprises a semiconductor laser, a computer, a rotary worktable, a stepping motor and a CCD camera. The semiconductor laser and the semiconductor refrigeration box are respectively fixed on the two horizontal arms of the rotary table, and the CCD camera is located in the semiconductor refrigeration box; the sample fixing clip is fixed on the bracket, and is located between the CCD camera and the semiconductor laser; the stepping motor drives the CCD The camera and semiconductor laser rotate around the center point of the vertical rod of the rotary table; the computer sends control signals to the rotary table and the CCD camera, receives the image data sent by the CCD camera, and analyzes and processes the image data. The system of the invention can freely control the size of the collected data and improve the imaging effect; it can realize the cooperative work of the rotation of the stepping motor and the shooting control of the cooling charge-coupled camera. In this way, the cooled charge-coupled camera can collect data stably and effectively during the rotation of the system.

The invention belongs to the field of medical optical coherent tomography technologies, and relates to a method for extracting information of a fluorescence tomography system for small animals. The method includes calibrating the tomography system and positioning the outer edge of a tomography cavity in a focal plane of a lens; respectively drawing luminous intensity distribution curves of emergent light of excitation light and fluorescence; selecting a range of a central angle theta of the tomography cavity and an information extraction region S mapped to a detected image; individually utilizing pixel data corresponding to a range of small luminous intensity values in the information extraction region S as a sub-region according to the luminous intensity distribution curves of the emergent light, and respectively utilizing pixel data corresponding to two-side ranges of high luminous intensity values as two sub-regions; solving an average value of measured data in each sub-region, utilizing each average value as an average detection value and reconstructing the image by the aid of all the extracted average detection values. The method has the advantages that computation can be simplified, and influence of two-side stray light can be eliminated.

The invention belongs to the technical field of medical imaging, and discloses a single-view-angle reflective near-infrared two-region fluorescence dynamic tomography system and method, and the system comprises a fluorescence imaging module, a CT imaging module, a central control module, a data processing module and an auxiliary module. The wide-field excitation light source is used for irradiating an imaging target, the collecting camera and the excitation light source are located on the same side, the imaging speed is greatly increased through reflection type imaging, and real-time fluorescence tomography can be obtained. According to the invention, near-infrared two-region imaging is adopted, so that the image resolution of three-dimensional reconstruction can be improved; a reflective fluorescence tomography technology is utilized, three-dimensional fluorescence tomography reconstruction is carried out only by using single-view-angle reflection fluorescence data, it is hopeful to achieve wide-field excitation to obtain high imaging speed, and real-time fluorescence tomography is obtained; the structure image of the sample is obtained by combining the single-view CT tomography technology of depth reconstruction, and the sample does not need to be rotated any more, so that the system complexity is reduced, and the imaging speed is improved at the same time.

The invention discloses a small animal multispectral fluorescence tomography system excited by narrow-beam X-ray and relates to the technical field of biomedicine images. The back portion of an X-ray constraint machine is provided with a fixing device. An animal bed is arranged in the fixing device. Reflecting devices are arranged on the two sides of the animal bed. An EMCCD camera is vertically arranged right above the animal bed. A smoothing wheel is arranged between the EMCCD camera and the animal bed. The smoothing wheel is provided with a plurality of circular holes evenly. Narrow-beam smoothing pieces are arranged in the circular holes. A stepping motor is arranged on one side of the fixing device. The stepping motor is connected with the animal bed. The back end of the fixing device is provided with an X-ray detector. Fluorescence materials in an object to be imaged are excited through the narrow-beam X-ray, accordingly, fluorescence signals of the surface of the object to be imaged and X-ray signals which penetrate through the object to be imaged are obtained, two kinds of obtained imaging information are subjected to reestablishing and fusion, and accordingly multi-model imaging is achieved.