65 results about "Tissue Embedding" patented technology

The invention disclosed herein improves upon existing tissue microarray technology by using frozen tissues embedded in tissue embedding compound as donor samples and arraying the specimens into a recipient block comprising tissue embedding compound. Tissue is not fixed prior to embedding, and sections from the array are evaluated without fixation or post-fixed according to the appropriate methodology used to analyze a specific gene at the DNA, RNA, and/or protein levels. Unlike paraffin tissue arrays which can be problematic for immunohistochemistry and for RNA in situ hybridization analyses, the disclosed methods allow optimal evaluation by each technique and uniform fixation across the array panel. The disclosed arrays work well for DNA, RNA, and protein analyses, and have significant qualitative and quantitative advantages over existing methods.

The present invention relates to a tissue embedding apparatus (1) for automatic embedding of at least one tissue sample (8). The tissue embedding apparatus (1) encompasses an input unit (2), an image acquisition unit (3), an embedding unit (4), at least one output unit (5, 6), and a control unit (7). A cassette (9) containing at least one tissue sample (8) is transferable to the input unit (2) of the tissue embedding apparatus (1). The tissue sample (8) is embeddable in an embedding medium by means of the embedding unit (4). The embedded tissue sample is outputtable with the at least one output unit (5). At least one image of the tissue sample (8) is acquirable with the image acquisition unit (3). In order for automated tissue embedding to be achievable even with the use of different cassettes, a tissue embedding apparatus (1) according to the present invention is characterized in that the image acquired of the tissue sample (8) is evaluatable, and the further processing of the tissue sample (8) in the tissue embedding apparatus (1) is definable as a function of the image evaluation.

The invention provides a dewaxing device of a tissue embedding machine; the dewaxing device is capable of continuously discharging wax, and comprises a main dewaxing cylinder and an auxiliary dewaxing cylinder, wherein the auxiliary dewaxing cylinder is provided with a first heating device; the bottom of the main dewaxing cylinder is provided with a wax outlet pipe; a wax conveying pipe is arranged between the main dewaxing cylinder and the auxiliary dewaxing cylinder; the auxiliary dewaxing cylinder is provided with a wax feeding hole and an air inlet; the auxiliary dewaxing cylinder is hermetically arranged; a second heating device is arranged at the pipe orifice of the wax outlet pipe. The dewaxing device of the tissue embedding machine has the beneficial effects that the auxiliary dewaxing cylinder can be used for providing standby wax for the main dewaxing cylinder in time, so that the liquid paraffin wax is continuously supplied without waiting; the heating device arranged at the pipe orifice of the wax outlet pipe can be used for separately heating paraffin wax at the pipe orifice, so that the paraffin wax is prevented from being cooled and hardened, the wax is smooth in flow, and wax blockage is avoided; the wax conveying pipe is controlled to convey the liquid paraffin wax by enabling the air inlet to be filled with high-pressure air, and a control valve is not arranged in the wax conveying pipe, so that wax leakage is avoided.

The invention provides a biological tissue embedding unit, an embedding template, an embedding tool, a dehydrating and embedding dual function machine and a using method. The biological tissue embedding unit comprises a multifunctional tissue embedding mould and a multifunctional tissue embedding box, wherein the longitudinal section of the multifunctional tissue embedding mould is in a concave structure; the opening of the concave structure is gradually reduced from top to bottom; a mould embedding positioning needle or a mould embedding positioning network or a mould embedding positioning secondary concave opening is formed at the bottom of the concave opening of the multifunctional tissue embedding mould, and a wax overflowing strip-shaped hole is formed in the mould wall of the longitudinal front end of the multifunctional tissue embedding mould; the multifunctional tissue embedding box is embedded into the multifunctional tissue embedding mould in a matched manner. The longitudinal section of the multifunctional tissue embedding box is in a trapezoidal structure, and an elastic positioning piece for compressing a biological tissue is further arranged in the multifunctional tissue embedding box. According to the invention, the errors are avoided, the medical accidents are prevented, a lot of time and labor intensity are saved, multiple embedding processes are saved and an embedding machine and a wax block refrigerator can be saved.

A container (2) for tissue preparation for the tissue embedding, having a base body (4), that is formed out cylindrical shaped side wall (5), a floor (6) and several bridges (50). The bridges (50) subdivide the container (2) in several sub-containers (52). The side wall (5) has a plurality of slit shaped openings (12), that extend essentially over the total height of the side wall (5).

The invention discloses a production method of a cell block paraffin section for fully presenting the position of a malignant cell. The method comprises the following steps: firstly, acquiring a primary cell sample and performing centrifugation sediment on the primary cell sample, so as to obtain a secondary cell sample; after the secondary cell sample is fixed, placing the secondary cell sample into a tissue dehydration box in a manner of vertical dividing from the middle for dehydration, transparentizing and waxing in sequence, so as to obtain a cell fixed sample; and vertically placing thecell fixed sampled into a tissue embedding box for embedding by taking a plane of section as a bottom surface, so as to form the section finally. Each section produced by the method can present the same cell hierarchical structure, so that the malignant cell appears at the same position of each section, a phenomenon that the malignant cell is located unreliably and even disappears in continuous sections is avoided, a comparative analysis when various antibodies are labeled in immunocytochemistry is facilitated, and cytopathology doctors can be effectively assisted to judge the source and the type of a malignant tumor, and therefore, the accuracy rate of cytopathological diagnosis is improved, a clinical application effect is good, and the practical value is high.

The invention is a matrix for preparing a tissue sample made of saline, albumin, gelatin, formaldehyde, a detergent, sucrose, lysine, and glutaraldehyde. The matrix is formed by first preparing a solution of saline, albumin, and gelatin, and then mixing the solution with formaldehyde, a detergent, and sucrose. Immediately prior to fixation of the tissue, a separately prepared lysine-glutaraldehyde mixture is added. The resulting matrix will harden rapidly and may be used to fix tissue samples for histological processing. In particular, a layer of the matrix may be poured into a mold and allowed to harden to form a base. One or more tissue samples may then be pinned to the base of matrix in the mold and then covered with the matrix. Once the matrix hardens, the pins and mold may be removed, and the tissue sample or samples embedded in the matrix are ready for sectioning.

The invention discloses a multifunctional pathological tissue embedding box, which is provided with a box body (1) for placing a tissue specimen and a box cover, wherein the box body (1) is a concave groove formed by a bottom surface and four side surfaces, the bottom surface is provided with louvers (2) and is further provided with at least two sinking wells (3), the inner diameter of each sinking well (3) is 0.5-2 cm, the depth is 0.2-1 mm, the well edge of each sinking well (3) is provided with an annular groove (4), the well bottom of the sinking well (3) is provided with a plurality of through holes (5), the distance between the through holes (5) is 0.2-1 mm, separated tissue placement chambers (6) are correspondingly arranged on the sinking wells (3), and the bottom end of the tissue placement chamber (6) is just inserted into the annular groove (4). According to the multifunctional pathological tissue embedding box, with the structure, multi-purpose-in-one-box can be achieved, dehydration embedding on conventional tissues, small tissues and gastrointestinal endoscope tissues can be achieved, the embedding efficiency of paraffin can be fundamentally improved, and the case analysis speed can be increased.

This invention is frozen tissue sample section embed apparatus used for life science experiment and medical pathological tissue examination. A multi-gauge multi-use tissue sample embed apparatus is comprised of a multi-gauge embedding frame, an embedding substrate, a spherical connector. By designing tissue sample embedding apparatus with different size and gauge, problems with existing technology of difficulty on embedding tissue and embryo in different shape and size can be conveniently solved via using single case, which effectively avoid confusion among different tissue and samples. The apparatus according to this invention can conveniently and rapidly carry out high quality freezing process can be carried out on embedded tissue and embryo as a special tissue embed-freeze apparatus, especially suitable for life science, pathology and anatomy, etc., which simultaneously bring new improvement for existing tissue embedding module.

The invention provides a method suitable for in-situ hybridization of a cherax quadricariratus gonadal tissue mRNA paraffin section, and belongs to the technical field of in-situ hybridization. The method comprises the steps of gonadal tissue embedding, paraffin sectioning, in-situ hybridization, and photographing and recording. The sequence of a probe used in in-situ hybridization is as shown inSEQ ID NO.3. The probe has clear specificity on tissue positioning of a dsx gene, so that the display effect on the mRNA level of the dsx gene is realized. The method for mRNA in-situ hybridization ofthe paraffin section is established in cherax quadricariratus to research expression modes of related genes in the gonad, and the expression location of the sex-related genes of the cherax quadricariratus in the gonad can be clearly described. The method avoids tissue shrinkage and cell deformation while the dewaxing effect of the paraffin section is good, so that the tissue is still kept in theoriginal position, the tissue morphology is kept complete, the enzymolysis effect can be improved, cell coloring is good, nucleoplasm is clear, and the in-situ hybridization accuracy is finally improved.

The invention discloses a cell three-dimensional culture matrigel wax embedding method. The method includes steps: (1) designing and manufacturing a slide which morphologically fits an internal storage space of an embedding box; (2) cutting off a matrigel three-dimensional culture tissue from a Transwell upper chamber, and fixing in paraformaldehyde in mass percent of 4-6% for 6-24h; (3) after successful fixing, taking the matrigel three-dimensional culture tissue out of paraformaldehyde, and enabling a collagen face of the matrigel three-dimensional culture tissue to be adsorbed on the slide;(4) putting the slide which morphologically fits the internal storage space of the embedding box and adsorbs the matrigel three-dimensional culture tissue into the embedding box in which gauze is laid; (5) dewatering, transparentizing and waxing; (6) adopting a tissue slicing knife for completely scrape off the tissue adsorbed on the slide from the slide, and embedding the tissue. In an embeddingprocess, tissue curling and shedding and cell falling are avoided. Therefore, the method is suitable for popularization and utilization.

The invention discloses a pathological liquid-based thin-layer cell tissue embedding machine and a using method thereof. The pathological liquid-based thin-layer cell tissue embedding machine comprises a centrifugal mechanism, the centrifugal mechanism comprises a rotating disc, the rotating disc comprises a disc body and a plurality of hanging plates evenly arranged on the peripheral side of thedisc body, protruding blocks matched with positioning grooves of a pod box are arranged on two sides of each hanging plate, and a plurality of through holes used for hanging cylindrical pods are formed in the edge of the disc body. The using method of the embedding machine comprises the following steps of: 1, determining a dosage threshold value of a cell tissue sample; 2, pretreating a first celltissue sample; 3, pretreating a second cell tissue sample; 4, selecting a required cell tissue sample detection type; 5, preparing a liquid-based thin-layer cell smear; and 6, preparing the cell embedding block. The problems of large instrument space occupation, high cost and the like due to the fact that various detection modes need various instruments and equipment are solved, the inspection time is saved, and popularization and application are facilitated.

The invention relates to a toluidine blue rapid staining method of brain tissue frozen section. The method comprises the following steps: appropriately trimming the treated experimental animal brain tissue, placing on a sample holder in a required direction, embedding the tissue as a cylinder by utilizing tinfoil, dropping in OTC embedding glue, and quickly freezing in a refrigerator at -80 DEG C;taking out the frozen tissue section, and then placing in AF stationary liquid to immobilize immediately; performing washing: placing the immobilized frozen section in distilled water to wash for 5-7s; performing toluidine blue staining: placing the washed frozen section in a toluidine blue solution, which is preheated to 50-60, to stain; performing washing: placing the stained section in the distilled water, washing for 8-14s until the flooding is washed; performing dehydrating: placing the section in 80% of alcohol to dehydrate for 1min; performing color separation: placing the dehydrated section in the 95% of alcohol to perform color separation for 20-30s; performing dehydrating: quickly placing the color-separated section in the anhydrous alcohol to dehydrate for 10-20s; and performing transparency and mounting: placing the section in the xylene-alcohol mixed solution for 3-8s, and then placing in the pure xylene for 5-10s, and then performing the mounting.

The invention discloses a biological tissue embedding machine. The biological tissue embedding machine comprises a base, a wax melting box and a manufacturing table; a strip-shaped groove is formed inthe upper surface of the base in the length direction; a lead screw is rotationally connected into the strip-shaped groove; a lead screw nut seat is in threaded connection with the lead screw; the manufacturing table is arranged on the upper surface of the base and fixedly welded to the lead screw nut seat; a servo motor driving the lead screw to rotate is fixed to one end, in the length direction, of the base through bolts. According to the biological tissue embedding machine of the present invention, a filter cover is arranged on a wax guide pipe for conveying wax liquid; a filter screen cover is arranged in the filter cover; the wax liquid enters the filter screen cover to be filtered; the wax liquid passing through the filter screen cover can be discharged through the wax guide pipe;impurities in the wax liquid can be filtered and collected through the filter screen cover and then discharged through a slag discharge pipe; and therefore, foreign matters are effectively prevented from polluting specimens; and the the slag discharge pipe is designed, so that the cleaning of the filter screen cover can be facilitated.

A heating and cooling device (3) for an apparatus (1) for tissue preparation for the tissue embedding is disclosed. The apparatus (1) has a free space (13) for the accommodation of a transport plate (15). The apparatus (1) is surrounded by a housing (5) which defines an upper side (8) and a heating and cooling device (3) that is provided on the upper side (8) of the housing (5), and the heating and cooling device (3) has a reception (23) with at least one chamber (32) for the accommodation of at least one processing container (17). The heating and cooling device (3) is pivotable with respect to the upper side (8) of the housing (5).

The invention provides an automatic treatment device and method for tissue sample dehydration and embedding. The device comprises a three-dimensional moving device, a rotary driving device, a disc container, a multi-groove liquid changing workbench and a tissue embedding mold box assembly. The tissue embedding mold box assembly comprises an embedding mold box upper cover, an embedding mold box anda tissue positioning support located between the embedding mold box upper cover and the embedding mold box. The tissue positioning support is used for seamlessly attaching biological tissue to the bottom of the embedding mold box. The disc container is used for accommodating at least one tissue embedding mold box assembly; the multi-groove liquid changing workbench comprises at least one container containing molten embedded wax liquid. The three-dimensional moving device is used for driving the disc container to move up and down in each container; and the rotary driving device is used for driving the disc container to rotate in the container. The whole process is automatically carried out, and compared with the prior art, samples do not need to be manually transferred among several different machines, so that the working efficiency is greatly improved.

The invention relates to tissue embedding solution and a tissue slice preparation method. The tissue embedding solution comprises resin monomers, a plasticizer, a catalyzer, an accelerator and a defoamer; according to the volume percentage of the tissue embedding solution, the resin monomers include 30-35% of a hydrophilic resin monomer, 55-60% of a first hydrophobic resin monomer and 5-10% of a second hydrophobic resin monomer; the plasticizer is 5-8% in the volume percentage of the tissue embedding solution; the concentration of the catalyzer is 0.4g/100mL; the concentration of the accelerator is 40muL/100mL to 50muL/100mL; and the concentration of the defoamer is 200muL/100mL to 400muL/100mL. After being embedded by the tissue embedding solution, a tissue to be analyzed is sliced; and atissue slice difficult to deform can be prepared.

The invention relates to a mold for preparing a wax block used for embedding pathologic tissue. The mold comprises a frame and a mold core, wax block grooves are formed in the upper surface of the mold core, guiding holes penetrating the mold core are arranged in the bottom centers of the wax block grooves respectively, each guiding hole is internally provided with a matched and vertically penetrated push rod, horizontal top plates are fixed to the upper ends of the push rods respectively, the lower portions of the push rods are sleeved by springs respectively, and castings extending vertically and upwardly are fixed to the centers of the upper surfaces of the top plates respectively. The prepared wax block is complete in the morphological structure and provided with a central hole, relatively small or thin tissue can be placed in the hole during use, and then liquid wax is filled into the hole to embed the tissue, the hole can limit the tissue movement and prevent the tissue from inclination, thus, the orientation of the tissue is fixed, and a user can obtain an ideal position during slicing, and mechanical damage on the tissue when the orientation of the tissue is adjusted by repeatedly clamping the tissue by tweezers can be effectively avoided.