The invention relates to the technical field of
food processing, and particularly discloses a preparation method of high-purity rubusoside, which comprises the following steps: (1) taking a sweet
tea leaf raw material, crushing, and extracting with water to obtain a sweet tea
powder extracting solution; (2) performing
filtration,
macroporous resin purification,
ion exchange resin decoloration, concentration,
drying and crushing on the sweet tea
powder extracting solution to obtain a rubusoside crude product; (3) dissolving the rubusoside crude product in a 65-75%
ethanol water solution, cooling to 8 DEG C from 60 DEG C, carrying out gradient
crystallization, carrying out heat preservation for 1-2 hours, filtering, and
drying to obtain a primary
crystallization product; and (4) dissolving the primary
crystallization product in a 90-95%
ethanol water solution, preserving heat at 60 DEG C for 1.5-2.5 h, cooling to 25 DEG C, preserving heat for 3.5-4.5 h, finally cooling to 8 DEG C, preserving heat for 11-13 h, filtering, and
drying to obtain the product. Through two-step gradient crystallization,
solvent concentration control,
temperature gradient design and heat preservation time optimization, the product purity, the crystallization quality and the storage stability are remarkably improved.