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Fluorescence resonance energy transfer polystyrene fluorescent microsphere and preparation method thereof

A technology of fluorescence resonance energy and polystyrene microspheres, which is applied in chemical instruments and methods, measuring devices, luminescent materials, etc., can solve the problems of inconvenient detection and distinction of excitation light and emission light, and achieve uniform particle size and good anti-interference Ability, the effect of strong fluorescent signal

Inactive Publication Date: 2019-02-15
HANGZHOU LAIHE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the small Stockton shift of traditional fluorescent microspheres, the peak difference between fluorescence excitation and emission is mostly within 50nm, which brings inconvenience to the detection and distinction of excitation light and emission light

Method used

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  • Fluorescence resonance energy transfer polystyrene fluorescent microsphere and preparation method thereof
  • Fluorescence resonance energy transfer polystyrene fluorescent microsphere and preparation method thereof
  • Fluorescence resonance energy transfer polystyrene fluorescent microsphere and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0031] Example 1: Preparation of Fluorescence Resonance Energy Transfer Polystyrene Fluorescent Microspheres by Swelling Method

[0032] (1) Weigh 0.1 g of monodisperse carboxypolystyrene microspheres into 5 mL of 0.5% SDS aqueous solution, and sonicate for 10 min to disperse the microspheres evenly.

[0033] (2) Centrifuge at 5000R / min for 5min, remove the supernatant, reconstitute with 5 mL of 0.5% SDS aqueous solution and sonicate for 10min; to obtain a dispersion system of microspheres;

[0034] (3) Weigh 5 mg rhodamine B n-octyl ester and Nile Blue A each, dissolve in 1 mLCH 2 Cl 2 Obtain fluorescent mixed solution;

[0035] (4) Add the fluorescent mixed solution to the dispersion system of microspheres, sonicate for 10 minutes, shake vigorously at room temperature for 1 hour, let stand at 2-8 degrees for 12 hours, and then spin evaporate at 30°C for 2 hours to remove CH 2 Cl 2 ;

[0036] (5) Centrifuge at 5000R / min for 5min, remove the supernatant, redissolve in d...

Embodiment 2

[0037] Example 2: CRP (C-reactive protein) rapid quantitative detection reagent

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Abstract

The invention discloses a fluorescence resonance energy transfer polystyrene fluorescent microsphere. Two fluorescent materials such as rhodamine B n-octyl ester and nile blue A are embedded in polystyrene microspheres, and by virtue of fluorescence resonance energy transfer, the purpose of prolonging the Stockton displacement is achieved. Exciting light and emitted light can be easily distinguished, and interference of the exciting light on detection and interference on a fluorescence signal in a sample can be reduced.

Description

technical field [0001] The invention relates to the technical field of cross-linked polymer and fluorescent substance embedding, in particular to a preparation method and application of fluorescent resonance energy transfer polystyrene fluorescent microspheres. Background technique [0002] Fluorescent microspheres are solid particles with diameters ranging from nanometers to micrometers (0.01-10μm) loaded with fluorescent substances, which can be excited to fluoresce when stimulated by external energy. Polystyrene fluorescent microspheres have been widely used in cell labeling, drug high-throughput screening, immunoassay, and standard measurement due to their good monodispersity, stable optical properties, and rich surface functional groups. One of the important applications is immunoassay and rapid diagnosis based on fluorescent microspheres. [0003] Polystyrene fluorescent microspheres have been widely used in cell labeling, drug high-throughput screening, immunoassay, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06G01N33/533G01N33/543G01N33/58G01N33/68
CPCC09K11/06C09K2211/1033C09K2211/1088G01N33/533G01N33/54313G01N33/582G01N33/68
Inventor 葛秀龙欧阳云叶肖俊
Owner HANGZHOU LAIHE BIOTECH CO LTD
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