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Fluorescence detection method for detecting cocaine by use of oligonucleotide and graphene oxide

An oligonucleotide and fluorescence detection technology, which is applied in the field of fluorescence detection, can solve the problems of low sensitivity and expensive detection instruments, and achieve high specificity and sensitivity, and the effect of simple detection methods

Active Publication Date: 2013-03-27
HUBEI CHINA TOBACCO IND +1
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Problems solved by technology

[0006] The purpose of the present invention is to provide a fluorescent detection method that utilizes oligonucleotides and graphene oxide to detect cocaine, which has higher specificity and sensitivity

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  • Fluorescence detection method for detecting cocaine by use of oligonucleotide and graphene oxide
  • Fluorescence detection method for detecting cocaine by use of oligonucleotide and graphene oxide

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Embodiment

[0028] 1) Add 1 μL of fluorescein FAM-labeled oligonucleotide probe with a concentration of 1.8 μmol / L to 1 mL of Tris-HCl buffer solution, then add 0.6 mL of water for fluorescence detection, and record the oligonucleotide The fluorescence emission band of the probe, and the results of detection of fluorescence intensity are as follows: figure 2 as shown in a;

[0029] 2) Then add 3 μL of graphene oxide solution, and the oligonucleotide probes are quickly adsorbed to the surface of graphene oxide at room temperature, forming oligonucleotide / graphene oxide complexes, and quenching the fluorescence signal of fluorescein FAM. off, the time of fluorescence quenching is 2 minutes, and the results of detecting fluorescence intensity under the same fluorescence spectrum measurement conditions are as follows: figure 2 as shown in b;

[0030] 3) Add 1 μL of complementary probes with a concentration of 1.8 μmol / L to the oligonucleotide / graphene oxide complex, and the complementary ...

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Abstract

The invention discloses a fluorescence detection method for detecting cocaine by use of oligonucleotide and graphene oxide. The method comprises the following steps of: adding a dye-marked oligonucleotide probe into a buffer solution; adding graphene oxide as a solid-phase carrier so as to adsorb the oligonucleotide probe onto the surface of the graphene oxide and form an oligonucleotide / graphene oxide compound, and quenching a fluorescence signal of the dye; adding a complementary probe for reaction, wherein a double-strand secondary structure is formed through the base combination effect between the complementary probe and the oligonucleotide probe so as to recover fluorescence; and adding cocaine for reaction, wherein three stem-like secondary structures are formed through the specific effect between the cocaine and the complementary probe so that the oligonucleotide probe is re-combined to the surface of the graphene oxide and the fluorescence is quenched. According to the invention, the aim of cocaine detection is achieved by detecting the change of fluorescence intensity in the solution; and the detection method is simple and quick, and has relatively high specificity and sensitivity.

Description

technical field [0001] The invention relates to a detection method of cocaine, in particular to a fluorescence detection method using oligonucleotides and graphene oxide to detect cocaine. technical background [0002] Cocaine is one of the most important alkaloids isolated from coca leaves. It is a central nervous system stimulant and a powerful local anesthetic. Cocaine is one of the common drugs. Smoking cocaine will produce strong psychological dependence and cause great harm to the body, causing social security problems. Therefore, it is of great significance to find a fast, simple and highly sensitive method to detect cocaine, which is a hot topic in current research. [0003] For example, the publication No. CN 101948907 A "A Method for Improving Cocaine Detection Sensitivity" uses gold nanoparticles, which can observe color changes, and can use optical and electrochemical signals to determine the presence of cocaine. The advantage of this patent is that it can be d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
Inventor 苗立坤喻世涛黄龙朱巍
Owner HUBEI CHINA TOBACCO IND
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